MX2021003847A - Analisis simultaneo de proteinas, nucleosomas y acidos nucleicos libres de celula, basado en secuenciacion, de una muestra biologica individual. - Google Patents

Analisis simultaneo de proteinas, nucleosomas y acidos nucleicos libres de celula, basado en secuenciacion, de una muestra biologica individual.

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Publication number
MX2021003847A
MX2021003847A MX2021003847A MX2021003847A MX2021003847A MX 2021003847 A MX2021003847 A MX 2021003847A MX 2021003847 A MX2021003847 A MX 2021003847A MX 2021003847 A MX2021003847 A MX 2021003847A MX 2021003847 A MX2021003847 A MX 2021003847A
Authority
MX
Mexico
Prior art keywords
cell
sequencing
sample
biological sample
free dna
Prior art date
Application number
MX2021003847A
Other languages
English (en)
Spanish (es)
Inventor
Patrick A Arensdorf
Samuel Levy
Damek Spacek
Christopher E Ellison
Original Assignee
Bluestar Genomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bluestar Genomics Inc filed Critical Bluestar Genomics Inc
Publication of MX2021003847A publication Critical patent/MX2021003847A/es

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1065Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804Nucleic acid analysis using immunogens

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
MX2021003847A 2018-10-04 2019-10-03 Analisis simultaneo de proteinas, nucleosomas y acidos nucleicos libres de celula, basado en secuenciacion, de una muestra biologica individual. MX2021003847A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862741473P 2018-10-04 2018-10-04
PCT/US2019/054582 WO2020072829A2 (en) 2018-10-04 2019-10-03 Simultaneous, sequencing-based analysis of proteins, nucleosomes, and cell-free nucleic acids from a single biological sample

Publications (1)

Publication Number Publication Date
MX2021003847A true MX2021003847A (es) 2021-05-27

Family

ID=68296828

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021003847A MX2021003847A (es) 2018-10-04 2019-10-03 Analisis simultaneo de proteinas, nucleosomas y acidos nucleicos libres de celula, basado en secuenciacion, de una muestra biologica individual.

Country Status (9)

Country Link
US (1) US12540319B2 (https=)
EP (1) EP3861132A2 (https=)
JP (1) JP2022504078A (https=)
CN (1) CN113166796A (https=)
AU (1) AU2019354789A1 (https=)
CA (1) CA3114606A1 (https=)
MX (1) MX2021003847A (https=)
SG (1) SG11202102954QA (https=)
WO (1) WO2020072829A2 (https=)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113517026B (zh) * 2021-06-16 2022-08-19 苏州拉索生物芯片科技有限公司 应用于生物制品的标签序列的生成方法、系统、智能终端及计算机可读存储介质
EP4370711A4 (en) * 2021-07-12 2025-07-02 Univ Pennsylvania MODIFIED ADAPTERS FOR ENZYMATIC DEAMINATION OF DNA AND METHODS OF USE THEREOF FOR EPIGENETIC SEQUENCING OF FREE AND IMMOBILIZED DNA
US20240301466A1 (en) * 2021-07-15 2024-09-12 University Of Washington Efficient duplex sequencing using high fidelity next generation sequencing reads
US20230086611A1 (en) * 2021-09-23 2023-03-23 Genomic Testing Cooperative, LCA Compositions and Methods for Targeted NGS Sequencing of cfRNA and cfTNA
CN118139987A (zh) * 2021-09-23 2024-06-04 基因组检测合作社公司 用于cfrna和cftna靶向ngs测序的组合物和方法
WO2023097295A1 (en) * 2021-11-24 2023-06-01 Alida Biosciences, Inc. Rna and dna analysis using engineered surfaces
CN114934110A (zh) * 2022-06-15 2022-08-23 江西烈冰生物科技有限公司 用于获取基因表达的原始位置的生物芯片、试剂盒及方法
AU2023384167A1 (en) * 2022-11-23 2025-04-24 Alida Biosciences, Inc. Chromatin profiling compositions and methods
WO2024112816A1 (en) 2022-11-23 2024-05-30 University Of Iowa Research Foundation Therapeutic compounds and methods
CN116403645B (zh) * 2023-03-03 2024-01-09 阿里巴巴(中国)有限公司 转录因子结合位点的预测方法及装置
WO2026024692A1 (en) * 2024-07-24 2026-01-29 Guardant Health, Inc. Direct methylation single-base resolution sequencing of enriched unmethylated dna

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5712126A (en) 1995-08-01 1998-01-27 Yale University Analysis of gene expression by display of 3-end restriction fragments of CDNA
US6287825B1 (en) 1998-09-18 2001-09-11 Molecular Staging Inc. Methods for reducing the complexity of DNA sequences
US20070020640A1 (en) 2005-07-21 2007-01-25 Mccloskey Megan L Molecular encoding of nucleic acid templates for PCR and other forms of sequence analysis
ATE551349T1 (de) 2005-11-30 2012-04-15 Epict Technologies Corp Methode unter verwendung von reversibel blockierten markierungsoligonukleotiden
EP2164985A4 (en) 2007-06-01 2014-05-14 454 Life Sciences Corp SYSTEM AND METHOD FOR IDENTIFYING INDIVIDUAL SAMPLES FROM A MULTIPLEX MIXTURE
US20090093378A1 (en) 2007-08-29 2009-04-09 Helen Bignell Method for sequencing a polynucleotide template
US8268564B2 (en) 2007-09-26 2012-09-18 President And Fellows Of Harvard College Methods and applications for stitched DNA barcodes
WO2010037001A2 (en) 2008-09-26 2010-04-01 Immune Disease Institute, Inc. Selective oxidation of 5-methylcytosine by tet-family proteins
US20100323348A1 (en) 2009-01-31 2010-12-23 The Regents Of The University Of Colorado, A Body Corporate Methods and Compositions for Using Error-Detecting and/or Error-Correcting Barcodes in Nucleic Acid Amplification Process
EP2816111B1 (en) 2009-08-14 2016-04-13 Epicentre Technologies Corporation Methods, compositions, and kits for generating rRNA-depleted samples or isolating rRNA from samples
WO2011127136A1 (en) 2010-04-06 2011-10-13 University Of Chicago Composition and methods related to modification of 5-hydroxymethylcytosine (5-hmc)
GB201107863D0 (en) * 2011-05-11 2011-06-22 Olink Ab Method and product
ES2609845T3 (es) * 2011-07-29 2017-04-24 Cambridge Epigenetix Limited Métodos para la detección de modificación de nucleótidos
WO2013113012A2 (en) 2012-01-27 2013-08-01 The Board Of Trustees Of The Leland Stanford Junior University Methods for profiling and quantitating cell-free rna
GB201303575D0 (en) 2013-02-28 2013-04-10 Singapore Volition Pte Ltd Method for detecting histone modifications in nucleosomes
CN105745334B (zh) 2013-09-30 2019-12-10 吴迪 通过使用邻近条形编码分析分子复合物的概况的方法
EP4389888A3 (en) 2013-10-17 2024-09-04 Takara Bio USA, Inc. Methods for adding adapters to nucleic acids and compositions for practicing the same
US10760120B2 (en) 2015-01-23 2020-09-01 Qiagen Sciences, Llc High multiplex PCR with molecular barcoding
US10844428B2 (en) * 2015-04-28 2020-11-24 Illumina, Inc. Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS)
US11092607B2 (en) * 2015-10-28 2021-08-17 The Board Institute, Inc. Multiplex analysis of single cell constituents
MX391039B (es) 2016-04-07 2025-03-21 Univ Leland Stanford Junior Diagnostico no invasivo por medio de la secuenciacion del adn libre de celulas 5-hidroxi-metilado.
US20170298422A1 (en) 2016-04-18 2017-10-19 The Board Of Trustees Of The Leland Stanford Junior University Simultaneous single-molecule epigenetic imaging of dna methylation and hydroxymethylation
US10093986B2 (en) 2016-07-06 2018-10-09 Youhealth Biotech, Limited Leukemia methylation markers and uses thereof
WO2018031897A1 (en) * 2016-08-12 2018-02-15 Cdi Laboratories, Inc. Compositions and methods for analyzing nucleic acids associated with an analyte
CN106367485B (zh) 2016-08-29 2019-04-26 厦门艾德生物医药科技股份有限公司 一种用于检测基因突变的多定位双标签接头组及其制备方法和应用
US20180080021A1 (en) 2016-09-17 2018-03-22 The Board Of Trustees Of The Leland Stanford Junior University Simultaneous sequencing of rna and dna from the same sample
WO2019160994A1 (en) * 2018-02-14 2019-08-22 Bluestar Genomics, Inc. Methods for the epigenetic analysis of dna, particularly cell-free dna

Also Published As

Publication number Publication date
CN113166796A (zh) 2021-07-23
WO2020072829A3 (en) 2020-08-13
US20210380971A1 (en) 2021-12-09
JP2022504078A (ja) 2022-01-13
WO2020072829A2 (en) 2020-04-09
AU2019354789A1 (en) 2021-05-27
SG11202102954QA (en) 2021-04-29
EP3861132A2 (en) 2021-08-11
CA3114606A1 (en) 2020-04-09
US12540319B2 (en) 2026-02-03

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