MX2019014137A - Nucleotidos etiquetados y usos de los mismos. - Google Patents

Nucleotidos etiquetados y usos de los mismos.

Info

Publication number
MX2019014137A
MX2019014137A MX2019014137A MX2019014137A MX2019014137A MX 2019014137 A MX2019014137 A MX 2019014137A MX 2019014137 A MX2019014137 A MX 2019014137A MX 2019014137 A MX2019014137 A MX 2019014137A MX 2019014137 A MX2019014137 A MX 2019014137A
Authority
MX
Mexico
Prior art keywords
nucleotide
labeled nucleotides
redox
electrically conductive
conductive channel
Prior art date
Application number
MX2019014137A
Other languages
English (en)
Inventor
Jeffrey Mandell
Steven Barnard
John Moon
Bacigalupo Maria Candelaria Rogert
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of MX2019014137A publication Critical patent/MX2019014137A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
    • G01N27/3277—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction being a redox reaction, e.g. detection by cyclic voltammetry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0663—Whole sensors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials

Abstract

Un nucleótido etiquetado incluye un nucleótido, una molécula de enlace unida a un grupo fosfato del nucleótido, y una etiqueta de carga activa a reducción-oxidación unido a la molécula de enlace. La etiqueta de carga activa a reducción-oxidación se va a oxidar o reducir por un canal eléctricamente conductor cuando se mantiene en proximidad de una zona de detección del canal eléctricamente conductor.
MX2019014137A 2018-02-16 2019-02-13 Nucleotidos etiquetados y usos de los mismos. MX2019014137A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862710465P 2018-02-16 2018-02-16
PCT/US2019/017830 WO2019160937A1 (en) 2018-02-16 2019-02-13 Labeled nucleotides and uses thereof

Publications (1)

Publication Number Publication Date
MX2019014137A true MX2019014137A (es) 2021-01-29

Family

ID=67618753

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2019014137A MX2019014137A (es) 2018-02-16 2019-02-13 Nucleotidos etiquetados y usos de los mismos.

Country Status (15)

Country Link
US (1) US11542550B2 (es)
EP (1) EP3752514A4 (es)
JP (2) JP6987898B2 (es)
KR (1) KR102415039B1 (es)
CN (1) CN111051324A (es)
AU (1) AU2019222685B2 (es)
BR (1) BR112019025493A2 (es)
CA (1) CA3065958C (es)
IL (1) IL270920A (es)
MX (1) MX2019014137A (es)
PH (1) PH12019502907A1 (es)
SA (1) SA519410842B1 (es)
SG (1) SG11201911570WA (es)
WO (1) WO2019160937A1 (es)
ZA (1) ZA201907988B (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102429576B1 (ko) * 2020-04-29 2022-08-03 경희대학교 산학협력단 전도성 태그를 이용한 dna 염기서열을 식별하는 방법 및 장치

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19533093C1 (de) * 1995-09-07 1996-12-05 Deutsches Krebsforsch Metallocen-Phosphoramidit-Konjugate, Verfahren zu ihrer Herstellung sowie deren Verwendung
US6869764B2 (en) * 2000-06-07 2005-03-22 L--Cor, Inc. Nucleic acid sequencing using charge-switch nucleotides
EP2226330B1 (en) * 2000-06-07 2013-09-18 Pacific Biosciences of California, Inc. Charge-switch nucleotides
US20030104386A1 (en) * 2001-08-31 2003-06-05 The Regents Of The University Of California Methods for the specific detection of redox-active tags and the use thereof for capillary gel electrophoresis and DNA sequencing
WO2009006445A2 (en) 2007-06-29 2009-01-08 Applied Biosystems Systems and methods for electronic detection with nanofets
US8372585B2 (en) * 2007-12-31 2013-02-12 Intel Corporation Electronic sensing for nucleic acid sequencing
US8563240B2 (en) * 2008-12-31 2013-10-22 Intel Corporation Nucleic acid sequencing and electronic detection
WO2013056241A2 (en) * 2011-10-14 2013-04-18 Pacific Biosciences Of California, Inc. Real-time redox sequencing
DK3460075T3 (da) * 2014-07-15 2021-02-01 Illumina Inc Biokemisk aktiveret elektronisk anordning
BR112017023189A2 (pt) * 2015-05-12 2018-07-31 Illumina Inc aparelho de efeito de campo e métodos para sequenciar ácidos nucleicos
WO2017024017A1 (en) * 2015-08-06 2017-02-09 Pacific Biosciences Of California, Inc. Systems and methods for selectively addressing sparsely arranged electronic measurement devices
EP4219506A1 (en) 2015-11-20 2023-08-02 Pacific Biosciences Of California, Inc. Protected dye-labeled reagents
RU2637310C1 (ru) 2016-06-08 2017-12-04 Федеральное Государственное Бюджетное Учреждение Науки Институт Молекулярной Биологии Им. В.А. Энгельгардта Российской Академии Наук (Имб Ран) Флуоресцентно-меченые дезоксиуридинтрифосфаты
CN109790578B (zh) * 2016-10-05 2024-04-09 豪夫迈·罗氏有限公司 使用纳米晶体管的核酸测序

Also Published As

Publication number Publication date
AU2019222685B2 (en) 2021-08-19
EP3752514A1 (en) 2020-12-23
EP3752514A4 (en) 2021-11-03
JP6987898B2 (ja) 2022-01-05
RU2019139633A3 (es) 2021-06-07
PH12019502907A1 (en) 2020-10-19
AU2019222685A1 (en) 2019-12-19
US11542550B2 (en) 2023-01-03
KR102415039B1 (ko) 2022-06-30
IL270920A (en) 2020-01-30
JP2022046478A (ja) 2022-03-23
SG11201911570WA (en) 2020-01-30
ZA201907988B (en) 2022-03-30
SA519410842B1 (ar) 2022-11-14
CN111051324A (zh) 2020-04-21
JP2020536487A (ja) 2020-12-17
WO2019160937A1 (en) 2019-08-22
BR112019025493A2 (pt) 2020-09-01
RU2019139633A (ru) 2021-06-07
US20200157618A1 (en) 2020-05-21
KR20200023281A (ko) 2020-03-04
CA3065958A1 (en) 2019-08-22
CA3065958C (en) 2023-01-03

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