MX2016001187A - Metodo para la produccion recombinante de un polipeptido en celulas procariotas. - Google Patents

Metodo para la produccion recombinante de un polipeptido en celulas procariotas.

Info

Publication number
MX2016001187A
MX2016001187A MX2016001187A MX2016001187A MX2016001187A MX 2016001187 A MX2016001187 A MX 2016001187A MX 2016001187 A MX2016001187 A MX 2016001187A MX 2016001187 A MX2016001187 A MX 2016001187A MX 2016001187 A MX2016001187 A MX 2016001187A
Authority
MX
Mexico
Prior art keywords
polypeptide
recombinant production
prokaryotic cells
coli
cultivating
Prior art date
Application number
MX2016001187A
Other languages
English (en)
Inventor
Christian Schantz
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of MX2016001187A publication Critical patent/MX2016001187A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y106/00—Oxidoreductases acting on NADH or NADPH (1.6)
    • C12Y106/99—Oxidoreductases acting on NADH or NADPH (1.6) with other acceptors (1.6.99)
    • C12Y106/99003—NADH dehydrogenase (1.6.99.3)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Se reporta en la presente un método para la producción recombinante de un polipéptido en E. coli que comprende las etapas de i) cultivar E. coli deficiente en NADH deshidrogenasa II que expresa el polipéotido y ii) recuperar el polipéptido a partir de la célula o el medio de cultivo.
MX2016001187A 2013-07-31 2014-07-29 Metodo para la produccion recombinante de un polipeptido en celulas procariotas. MX2016001187A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP13178739 2013-07-31
PCT/EP2014/066261 WO2015014829A1 (en) 2013-07-31 2014-07-29 Method for the recombinant production of a polypeptide in prokaryotic cells

Publications (1)

Publication Number Publication Date
MX2016001187A true MX2016001187A (es) 2016-04-29

Family

ID=48877159

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2016001187A MX2016001187A (es) 2013-07-31 2014-07-29 Metodo para la produccion recombinante de un polipeptido en celulas procariotas.

Country Status (12)

Country Link
US (1) US20160319319A1 (es)
EP (1) EP3027777A1 (es)
JP (1) JP2016524925A (es)
KR (1) KR20160034320A (es)
CN (1) CN105358705A (es)
BR (1) BR112015032141A2 (es)
CA (1) CA2915944A1 (es)
HK (1) HK1221742A1 (es)
MX (1) MX2016001187A (es)
RU (1) RU2016106950A (es)
SG (1) SG11201600725WA (es)
WO (1) WO2015014829A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024103025A1 (en) * 2022-11-11 2024-05-16 Rnaissance Ag Llc Fermentation based method for double stranded rna production

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5891669A (en) * 1997-03-17 1999-04-06 Novo Nordisk A/S, Novoalle, Methods for producing polypeptides in respiratory-deficient cells
JP4380029B2 (ja) * 2000-07-05 2009-12-09 味の素株式会社 微生物を利用した物質の製造法
JP4551725B2 (ja) * 2004-09-13 2010-09-29 Ｏｋｉセミコンダクタ株式会社 半導体装置の製造方法
US8877895B2 (en) * 2010-08-30 2014-11-04 Hoffmann-La Roche Inc. Prokaryotic expression construct

Also Published As

Publication number Publication date
US20160319319A1 (en) 2016-11-03
WO2015014829A1 (en) 2015-02-05
HK1221742A1 (zh) 2017-06-09
EP3027777A1 (en) 2016-06-08
CN105358705A (zh) 2016-02-24
JP2016524925A (ja) 2016-08-22
SG11201600725WA (en) 2016-02-26
CA2915944A1 (en) 2015-02-05
RU2016106950A (ru) 2017-09-04
BR112015032141A2 (pt) 2017-08-29
KR20160034320A (ko) 2016-03-29

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