MD98Y - Process for presowing treatment of sweet pepper seeds - Google Patents

Abstract

The invention relates to agriculture, in particular to a method of treating bell pepper seeds. The method, according to the invention, includes soaking the seeds before sowing for 12 hours in an aqueous solution containing 0.05…0.0005 g/L of glycosides, obtained from plants of Linaria vulgaris Mill. by extraction with ethanol and butanol and concentration. The result of the invention consists in increasing the resistance of bell pepper seedlings to stress caused by low temperatures.

Description

The invention relates to agriculture, in particular to a method for treating bell pepper seeds.

[0002] The method of treating bell pepper seeds before sowing is known, which includes treating the seeds before sowing with the use of steroid glycosides - melongoside and somelongoside to enhance the adaptogenic properties of bell pepper to low temperature conditions [1].

The disadvantage of the known process is that the use of the steroid glycoside is not sufficiently effective under low temperature conditions.

[0004] The problem solved by the present invention consists in increasing the resistance of bell pepper seedlings (Capsicum annuum L.) to low temperatures.

[0005] The essence of the invention lies in the fact that the process according to the invention includes soaking the seeds before sowing for 12 hours in an aqueous solution containing 0.05…0.0005 g/L of glycosides, obtained from plants of Linaria vulgaris Mill. by extraction with ethanol and butanol and concentration.

[0006] The result of the invention consists in increasing the resistance of bell pepper seedlings to stress caused by low temperatures.

[0007] The sum of linarosides was obtained from air-dried Linaria vulgaris Mill. (fam. Scrophulariaceae) plants by the method of extraction with water-saturated butanol, percolation of the concentrated extract on a silica gel column eluting with the system of 35 parts of chloroform, 30 parts of methanol and 5 parts of distilled water. The fractions containing glycosides were combined and concentrated. The finished product was dried under vacuum conditions (Maschenko N., Kintia P., Guriev A., Marchenko A., Basarello C., Piacente S., Pizza C. Glycosides from Linaria vulgaris Mill. Chemistry Journal of Moldova, v. 3; No. 2, 2008, p. 98-100).

[0008] Embodiment of the invention

[0009] The research was carried out on two varieties of bell pepper: Karlik and Agapovschii. Part of the dried seeds was soaked in aqueous solutions of the extract obtained from plants of Linaria vulgaris Mill. in the concentration limits of 0.05…0.0005 g/L (invention). For comparison, another part of the seeds was soaked in the aqueous solution of the steroid glycoside (melanoside) 3-O-{[ß-D-galactopyranosyl(1→4)-ß-D-glucopyranosyl(1→2)]-[ß-D-rhamnopyrano-

[0010] zyl(1→3)]-ß-D-glucopyranosyl}-[(25R)-furost-5-ene-3ß,22α,26-triol]-26-O-ß-D-glu-

[0011] copyranoside, obtained from eggplant seeds Solanum melongena L. by extraction with water upon heating at a concentration of 0.05 g/L (closest solution). Seeds soaked in distilled water served as control.

[0012] The repeatability of the experiment was 6 times, 100 seeds in each experiment.

[0013] The seeds of all variants were soaked for 12 hours, then washed in running water for 5 min and placed on sheets of filter paper, packed in rolls and grown for 14 days in a thermostat at a temperature of 25°C (optimal conditions).

[0014] Subsequently, the rolls with the formed seedlings were placed in the light and maintained for 7 days under the conditions of a 10-hour photoperiod (additional illumination with daylight lamps) and a temperature regime of 25…27°C (day) and 20…22°C (night).

[0015] In the phase of formation of the first true leaves, part of the rollers with seedlings was still maintained in the same optimal conditions (control), another part was maintained in a regime of sudden change of day and night temperatures (25°C during the day and 10°C at night) for 10 days (temperature stress). After the action of stress on the control and experimental seedlings, biometric analyses were made: length of the root, stem, first true leaf, resistance to cold.

[0016] The cold resistance was determined according to the formula:

[0017] Refrig = (O/K)x100%, where:

[0018] O - the value of the parameter after the action of stress;

[0019] K - the value of the parameter under optimal conditions.

The experimental results are given in the table.

[0021] Table

[0022] Influence of pre-sowing treatment of bell pepper seeds with glycoside solutions on seedling development under temperature stress conditions

variety

Variants Concentration of the sum of linarosides

Length (mm)

Cold resistance

root

stem

First true leaf

MM

% from control

MM

% from control

MM

% from control

root

First true leaf

Karlik

water (control)

65.9

18.2

0.6

72

20

the closest solution (melongozide)

63.7

96.7

18.0

98.9

0.8

133.3

70

21

Invention (amount of linaroside, g/L):

0.5

72.8

110.4

23.3

128.2

2.4

407.4

87

65

0.05

72.7

83.2

15.7

111.1

1.1

141.7

76.5

40.4

0.0005

46.4

70.5

19.3

106.0

0.3

47.6

65

9

The td* test

10.1

0.4

0.4

11

10

Agapov-skis

water (control)

87.3

14.1

0.8

70.8

21.2

the closest solution (melongozide)

88.6

101.5

12.8

90.8

0.65

81.2

66.5

15.1

Invention (amount of linaroside, g/L):

0.5

72.2

83.2

15.7

111.1

1.1

141.7

76.5

40.4

0.05

113.8

130.3

20.0

141.8

3.1

390.6

105.3

79.4

0.0005

101.9

116.8

22.7

160.9

3.2

394.2

102.6

71.3

The td* test

20.6

2.8

1.1

18.1

24.2

[0024] *td - coefficient of degree of authenticity

[0025] The analysis of the obtained data shows that soaking bell pepper seeds before sowing in glycoside solutions obtained from Linaria vulgaris Mill. plants increases the cold resistance of the root system by 15…35% and of the first true leaf by 45…58% compared to the control, the most significant effect being possessed by the solution with a concentration of 0.05… 0.005 g/L.

1. Балашова Н.Н., Балашова И.Т., Козарь Е.Г., Беспалько А.В., Пинчук Е.В., Shvets С.А., Кинтя П.К. The use of steroid glycosides to increase the adaptive properties of sweet pepper plants. Materials of reports Mezhd. Symposium Sovremennoe sostanie i perspektivky razvitiya selektsi i semenovodstva ovochnykh kultur, t. 2, Moscow, 2005, pp. 396...398

Claims (1)

1. Process for treating bell pepper seeds before sowing, which includes soaking them for 12 hours in an aqueous solution containing 0.05…0.0005 g/L of glycosides, obtained from Linaria vulgaris Mill. plants by extraction with ethanol and butanol and concentration.