MD844Z - Process for growing tobacco plants - Google Patents
Process for growing tobacco plants Download PDFInfo
- Publication number
- MD844Z MD844Z MDS20140059A MDS20140059A MD844Z MD 844 Z MD844 Z MD 844Z MD S20140059 A MDS20140059 A MD S20140059A MD S20140059 A MDS20140059 A MD S20140059A MD 844 Z MD844 Z MD 844Z
- Authority
- MD
- Moldova
- Prior art keywords
- seedlings
- variant
- tobacco
- seeds
- tobacco plants
- Prior art date
Links
- 235000002637 Nicotiana tabacum Nutrition 0.000 title claims abstract description 24
- 238000000034 method Methods 0.000 title claims abstract description 12
- 244000061176 Nicotiana tabacum Species 0.000 title claims abstract description 11
- 239000000243 solution Substances 0.000 claims abstract description 11
- 229930182470 glycoside Natural products 0.000 claims abstract description 7
- 239000007864 aqueous solution Substances 0.000 claims abstract description 6
- 150000002338 glycosides Chemical class 0.000 claims abstract description 6
- 241000167860 Linaria vulgaris Species 0.000 claims abstract description 5
- 238000009835 boiling Methods 0.000 claims abstract description 4
- 238000009331 sowing Methods 0.000 claims abstract description 4
- 238000000605 extraction Methods 0.000 claims abstract description 3
- 238000002791 soaking Methods 0.000 claims abstract description 3
- 241000208125 Nicotiana Species 0.000 abstract description 13
- GBMDVOWEEQVZKZ-UHFFFAOYSA-N methanol;hydrate Chemical compound O.OC GBMDVOWEEQVZKZ-UHFFFAOYSA-N 0.000 abstract description 2
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 9
- 229930185047 nicotianoside Natural products 0.000 description 6
- DRWIVSAUXCDFQK-UHFFFAOYSA-N Linarioside Natural products C12C(C)(O)C(Cl)C(O)C2(O)C=COC1OC1OC(CO)C(O)C(O)C1O DRWIVSAUXCDFQK-UHFFFAOYSA-N 0.000 description 5
- 230000035784 germination Effects 0.000 description 5
- 230000012010 growth Effects 0.000 description 5
- 230000018109 developmental process Effects 0.000 description 4
- 238000003306 harvesting Methods 0.000 description 4
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 208000015181 infectious disease Diseases 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 239000013543 active substance Substances 0.000 description 2
- 230000003698 anagen phase Effects 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- WVDDGKGOMKODPV-UHFFFAOYSA-N hydroxymethyl benzene Natural products OCC1=CC=CC=C1 WVDDGKGOMKODPV-UHFFFAOYSA-N 0.000 description 2
- 239000000741 silica gel Substances 0.000 description 2
- 229910002027 silica gel Inorganic materials 0.000 description 2
- 239000011877 solvent mixture Substances 0.000 description 2
- 238000005292 vacuum distillation Methods 0.000 description 2
- 238000001644 13C nuclear magnetic resonance spectroscopy Methods 0.000 description 1
- 238000005160 1H NMR spectroscopy Methods 0.000 description 1
- FQMSOJMRNXFKPU-UHFFFAOYSA-N 26-O-beta-D-glucopyransoyl-(25R)-5beta-furostane-3beta,22xi,26-triol 3-O-[alpha-L-rhamnopyranosyl (1->)]-beta-D-glucopyranside Natural products O1C(CO)C(O)C(O)C(O)C1OCC(C)CCC(C(C1C2(C)CCC3C4(C)CC5)C)(O)OC1CC2C3CCC4CC5OC1OC(CO)C(O)C(O)C1OC1OC(C)C(O)C(O)C1O FQMSOJMRNXFKPU-UHFFFAOYSA-N 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 244000178870 Lavandula angustifolia Species 0.000 description 1
- 239000004952 Polyamide Substances 0.000 description 1
- 241000561282 Thielaviopsis basicola Species 0.000 description 1
- 235000019445 benzyl alcohol Nutrition 0.000 description 1
- -1 benzyl alcohol glycosides Chemical class 0.000 description 1
- SIHHLZPXQLFPMC-UHFFFAOYSA-N chloroform;methanol;hydrate Chemical compound O.OC.ClC(Cl)Cl SIHHLZPXQLFPMC-UHFFFAOYSA-N 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000000855 fungicidal effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 229930182487 phenolic glycoside Natural products 0.000 description 1
- 150000007950 phenolic glycosides Chemical class 0.000 description 1
- 229920002647 polyamide Polymers 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 230000007226 seed germination Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000010898 silica gel chromatography Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 229930002534 steroid glycoside Natural products 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 238000004809 thin layer chromatography Methods 0.000 description 1
- 230000003253 viricidal effect Effects 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Manufacture Of Tobacco Products (AREA)
Abstract
Description
Invenţia se referă la agricultură, în particular la un procedeu de cultivare a plantelor de tutun. The invention relates to agriculture, in particular to a method of cultivating tobacco plants.
Este cunoscut procedeul de tratare a seminţelor de tutun cu soluţia apoasă de glicozida steroidică －nicotianozidă E, obţinută din seminţe de tutun, însă eficienţa acestui procedeu este relativ nesemnificativă [1]. The process of treating tobacco seeds with an aqueous solution of the steroid glycoside －nicotianoside E, obtained from tobacco seeds, is known, but the efficiency of this process is relatively insignificant [1].
Dezavantajul acestui procedeu constă în faptul că nicotianozida E se obţine doar din seminţe de tutun, din care motiv randamentul de obţinere a acestora este destul de mic. În afară de aceasta, plantele de tutun trebuie cultivate, astfel accesibilitatea materiei prime fiind destul de limitată. The disadvantage of this process is that nicotianoside E is only obtained from tobacco seeds, which is why the yield of obtaining them is quite low. In addition, tobacco plants must be cultivated, so the accessibility of the raw material is quite limited.
Problema pe care o rezolvă invenţia propusă constă în sporirea productivităţii, calităţii şi rezistenţei plantelor de tutun la fungi şi lărgirea asortimentului substanţelor biologic active de origine naturală cu acţiune fungicidă şi virotică. The problem solved by the proposed invention consists in increasing the productivity, quality and resistance of tobacco plants to fungi and expanding the assortment of biologically active substances of natural origin with fungicidal and virucidal action.
Procedeul de cultivare a plantelor de tutun, conform invenţiei, include înmuierea seminţelor înainte de semănat timp de 24 ore şi tratarea extraradiculară a răsadurilor în faza de urechiuşe cu soluţie apoasă de 0,01…0,1% de glicozide obţinute din Linaria vulgaris L. Mill (linariozide) prin extragere la fierbere cu soluţie hidrometanolică. The method of cultivating tobacco plants, according to the invention, includes soaking the seeds before sowing for 24 hours and extra-radicular treatment of the seedlings in the ear stage with an aqueous solution of 0.01...0.1% of glycosides obtained from Linaria vulgaris L. Mill (linariosides) by boiling extraction with hydromethanolic solution.
Rezultatul tehnic al invenţiei constă în sporirea energiei germinative a seminţelor şi a numărului de răsaduri pe 1 m2, plantulele fiind mai înalte, reducerea duratei de creştere a răsadurilor, reducerea numărului de răsaduri infectate cu putregaiul negru de rădăcină, ceea ce asigură sporirea recoltei şi a calităţii materiei prime de tutun. The technical result of the invention consists in increasing the germination energy of seeds and the number of seedlings per 1 m2, the seedlings being taller, reducing the growth period of seedlings, reducing the number of seedlings infected with black root rot, which ensures an increase in the harvest and quality of tobacco raw material.
Linariozidele sumare (LS) din L. vulgaris au fost obţinute în modul următor: masa vegetală de plantă (0,5 kg, toată planta) proaspătă a fost mărunţită şi extrasă prin fierbere cu soluţie de metanol în apă cu partea de masă de 60% în trei repetări. Extractele au fost unite, concentrate prin distilare în vid, iar reziduul apos a fost decantat cu benzen pentru înlăturarea balastului. Restul apos a fost concentrat în vid şi aplicat pe coloana cu poliamidă. Coloana a fost eluată cu apă, apoi cu amestecul de solvenţi apă-metanol (4:1 vol/vol). Au fost colectate porţiuni a câte 10 ml, care au fost analizate în strat subţire de silicagel în amestecul de solvenţi cloroform-metanol-apă (76:14:3 vol/vol/vol) şi unite conform constantelor cromatografice. În acest mod s-au obţinut mai multe fracţiuni îmbogaţite cu substanţe biologic active. Fracţiunea cu un conţinut predominant de glicozide cu valorile constantelor cromatografice Rf cuprinse între 0,54…0,69 a fost concentrată prin distilare în vid până la uscat. În reziduul uscat al fracţiunii respective au fost detectate cu ajutorul cromatografiei în strat subţire de silicagel 3 glicozide majore, structura cărora a fost stabilită după separare prin cromatografiere pe coloane cu silicagel cu ajutorul procedeelor fizico-chimice, spectroscopiei 1H şi 13C RMN. Astfel, în extractul sumar au fost identificate o glicozidă fenolică nouă, denumită de autori linariozida V, şi două glicozide ale alcoolului benzilic (Natalia Mashcenko, Pavel Kintia, Angela Gurev, ş. a. Glycosides from Linaria Vulgaris Mill. Chemisrty Journal of Moldova, V.3, Nr 2, 2008, p.98-100). The total linariosides (LS) from L. vulgaris were obtained as follows: the fresh plant mass (0.5 kg, whole plant) was ground and extracted by boiling with a 60% methanol solution in water in three repetitions. The extracts were combined, concentrated by vacuum distillation, and the aqueous residue was decanted with benzene to remove the ballast. The aqueous residue was concentrated in vacuo and applied to the polyamide column. The column was eluted with water, then with the water-methanol solvent mixture (4:1 vol/vol). Portions of 10 ml were collected, which were analyzed in a thin layer of silica gel in the chloroform-methanol-water solvent mixture (76:14:3 vol/vol/vol) and combined according to the chromatographic constants. In this way, several fractions enriched with biologically active substances were obtained. The fraction with a predominant content of glycosides with the values of the chromatographic constants Rf ranging from 0.54…0.69 was concentrated by vacuum distillation to dryness. In the dry residue of the respective fraction, 3 major glycosides were detected using silica gel thin layer chromatography, the structure of which was established after separation by silica gel column chromatography using physicochemical procedures, 1H and 13C NMR spectroscopy. Thus, in the summary extract, a new phenolic glycoside, called by the authors linarioside V, and two benzyl alcohol glycosides were identified (Natalia Mashcenko, Pavel Kintia, Angela Gurev, etc. Glycosides from Linaria Vulgaris Mill. Chemisrty Journal of Moldova, V.3, No. 2, 2008, p.98-100).
Exemplu de realizare a invenţiei Example of embodiment of the invention
Cercetările au fost efectuate în condiţii de câmp şi seră la plantele de tutun de soiul Moldavschii 456. The research was conducted under field and greenhouse conditions on tobacco plants of the Moldavschii 456 variety.
Înainte de semănat, o parte de seminţe au fost turnate în săculeţe de bumbac şi înmuiate timp de 24 ore în soluţii apoase de linariozide în concentraţiile de 0,01; 0,05; 0,1% (invenţia propusă); altă parte au fost înmuiate în soluţie apoasă de nicotianozidă cu concentraţia de 0,1% (cea mai apropiată soluţie). În calitate de martor au servit seminţele înmuiate în apă. Seminţele înmuiate au fost puse în termostat pentru încolţire la temperatura de 28°C timp de 3 zile. Before sowing, part of the seeds were poured into cotton bags and soaked for 24 hours in aqueous solutions of linariosides in concentrations of 0.01; 0.05; 0.1% (the proposed invention); another part was soaked in an aqueous solution of nicotianoside with a concentration of 0.1% (the closest solution). Seeds soaked in water served as a control. The soaked seeds were placed in a thermostat for germination at a temperature of 28°C for 3 days.
După încolţire, seminţele au fost semănate în seră pe parcele cu suprafaţa de 1m2 în patru repetări pentru fiecare variantă. În faza de urechiuşe, înainte de plantare, răsadurile de tutun au fost stropite cu aceleaşi substanţe şi în aceleaşi concentraţii ca şi seminţele. Pentru fiecare parcelă de 1m2 s-au utilizat 0,5 l de soluţie. Datele obţinute arată că concentraţia optimală este de 0,1%. After germination, the seeds were sown in the greenhouse on plots with an area of 1m2 in four repetitions for each variant. In the ear stage, before planting, the tobacco seedlings were sprayed with the same substances and in the same concentrations as the seeds. For each plot of 1m2, 0.5 l of solution was used. The data obtained show that the optimal concentration is 0.1%.
Rezultatele obţinute denotă că în rezultatul tratării seminţelor şi a răsadurilor în faza de urechiuşe cu soluţie de linariozide în concentraţie de 0,1% pe fondul infecţios sporeşte energia germinativă a seminţelor de două ori în raport cu martorul, se reduce durata de creştere a răsadurilor cu 5 zile, plantulele sunt mai înalte cu aproape 3 cm faţă de varianta martor, iar masa medie a plantulei sporeşte cu 29,8%. Totodată numărul de răsaduri standard de pe 1m2 depăşeşte cu 370 bucăţi varianta martorului şi cu 195 bucăţi varianta nicotianozidă. Pe fondul natural intensitatea dezvoltării putregaiului negru al rădăcinii (Thielaviopsis basicola) s-a redus aproape de 5 ori comparativ cu martorul şi de 2 ori comparativ cu cea mai apropiată soluţie, iar gradul de infectare a fost cuprins între 0-1 bal, ce arată că plantele tratate cu soluţii de linariozide au fost rezistente la patogenii din sol (vezi tab. 1). The results obtained show that as a result of treating seeds and seedlings in the ear stage with a 0.1% linarioside solution on an infectious background, the germination energy of the seeds increases twice compared to the control, the growth period of the seedlings is reduced by 5 days, the seedlings are almost 3 cm taller than the control variant, and the average mass of the seedling increases by 29.8%. At the same time, the number of standard seedlings per 1m2 exceeds the control variant by 370 pieces and the nicotianozide variant by 195 pieces. On a natural background, the intensity of development of black root rot (Thielaviopsis basicola) was reduced almost 5 times compared to the control and 2 times compared to the closest solution, and the degree of infection was between 0-1 score, which shows that plants treated with linarioside solutions were resistant to soil pathogens (see Table 1).
Tabelul 1 Table 1
Influenţa linariozidelor sumare asupra creşterii şi dezvoltării răsadurilor standard de tutun The influence of summary linariosides on the growth and development of standard tobacco seedlings
Nr d/r Varianta, concentraţia, % Energia germinativă a seminţelor, % Durata de creştere a răsadurilor, zile Înălţimea plantulelor, cm Răsaduri-standard, buc/m2 Masa plantulei, media de la 100 cm2, mg Intensitatea dezvoltării Gradul de infectare putregaiului negru al rădăcinii, % 1 Martor (H2O) 47 53 11,9 1000 2715 54,9±1,6 3 2 Nicotianozidă 0,1% 82 50 13,6 1195 3445 23,7±0,4 1-2 3 LS 0,01% 89 50 13,2 1280 3390 13,9±0,9 1 4 LS 0,05% 90 49 13,9 1310 3470 12,3±0,3 1 5 LS 0,1% 96 48 14,5 1370 3525 11,7±0,7 0-1No. d/r Variant, concentration, % Seed germination energy, % Seedling growth duration, days Seedling height, cm Standard seedlings, pcs/m2 Seedling mass, average from 100 cm2, mg Development intensity Black root rot infection degree, % 1 Control (H2O) 47 53 11.9 1000 2715 54.9±1.6 3 2 Nicotianoside 0.1% 82 50 13.6 1195 3445 23.7±0.4 1-2 3 LS 0.01% 89 50 13.2 1280 3390 13.9±0.9 1 4 LS 0.05% 90 49 13.9 1310 3470 12.3±0.3 1 5 LS 0.1% 96 48 14.5 1370 3525 11.7±0.7 0-1
Răsadurile au fost plantate în câmp pe parcele în 4 repetări pentru a crea ambele fonduri: natural şi infectat. Pentru crearea fondului infectat aspru fiecare parcelă a fost împărţită în două părţi: fond rigid şi fond natural. Pe parcursul vegetaţiei au fost notate fenofazele şi biometria plantelor; lungimea şi lăţimea frunzelor, înălţimea plantei şi numărul frunzelor recoltate. Rezultatele obţinute arată că la 45 de zile după plantare, tutunul în varianta LS cu concentraţia de 0,1% are o înălţime mai mare cu 7,5 cm faţă de varianta martor şi cu 1,3 cm mai mare faţă de varianta nicotianozidă. În faza creşterii intensive plantele, în aceeaşi variantă a LS, sunt cu 24,6 cm mai înalte faţă de varianta martor, iar faţă de varianta nicotianozidă - cu 7,8 cm. De asemenea, creşte numărul de frunze şi suprafaţa limbului foliar (tab. 2). The seedlings were planted in the field on plots in 4 replications to create both backgrounds: natural and infected. To create the severely infected background, each plot was divided into two parts: rigid background and natural background. During the vegetation, the phenophases and biometry of the plants were noted; leaf length and width, plant height and number of harvested leaves. The results obtained show that 45 days after planting, tobacco in the LS variant with a concentration of 0.1% has a height greater by 7.5 cm compared to the control variant and by 1.3 cm compared to the nicotianoside variant. In the intensive growth phase, the plants, in the same LS variant, are 24.6 cm higher than the control variant, and by 7.8 cm compared to the nicotianoside variant. Also, the number of leaves and the surface area of the leaf blade increase (Table 2).
Tabelul 2 Table 2
Influenţa linariozidelor sumare asupra creşterii şi dezvoltării plantelor de tutun The influence of summary linariosides on the growth and development of tobacco plants
Nr. d/r Varianta Înălţimea plantelor în câmp (media 25 plante) Frunze culese, buc. Suprafaţa limbului foliar, m2 După 45 zile de la plantare, cm Faza creşterii intensive, cm După culesul cinci, cm 1 Martor (H2O) 26,7 110,7 144,8 23,4 471,2 2 Nicotianozidă 0,1% 32,9 127,5 154,7 28,5 490,9 3 LS 0,01% 31,2 133,9 153,4 29,1 521,9 4 LS 0,05% 32,6 134,6 155,1 29,7 524,1 5 LS 0,1% 34,2 135,3 156,8 30,2 527,8No. d/r Variant Plant height in the field (average 25 plants) Leaves collected, pcs. Leaf blade area, m2 After 45 days from planting, cm Intensive growth phase, cm After the fifth harvest, cm 1 Control (H2O) 26.7 110.7 144.8 23.4 471.2 2 Nicotianoside 0.1% 32.9 127.5 154.7 28.5 490.9 3 LS 0.01% 31.2 133.9 153.4 29.1 521.9 4 LS 0.05% 32.6 134.6 155.1 29.7 524.1 5 LS 0.1% 34.2 135.3 156.8 30.2 527.8
Recoltarea frunzelor de tutun a fost realizată manual la maturitatea tehnică în 5 reprize. Datele obţinute la recoltarea roadei au fost supuse analizei statistice prin metoda dispersă (Dospehov, 1985). Tobacco leaves were harvested manually at technical maturity in 5 rounds. The data obtained during the harvest were subjected to statistical analysis using the dispersed method (Dospehov, 1985).
Tabelul 3 Table 3
Influenţa linariozidelor sumare asupra recoltei şi a calităţii materiei prime de tutun The influence of summary linariosides on the yield and quality of tobacco raw material
Nr. d/o Varianta Roada, t/ha Adaosul la roadă Randamentul calităţilor, % kg/ha faţă de % comparativ cu I sort II sort III sort Martor Fondul infectat natural, % 1 Martor (H2O) 1,56 - - 55,0 20,0 25,0 2 Nicotianozidă 0,1% 1,81 250 16,1 65,0 10,0 25,0 3 LS 0,01% 1,79 230 14,7 60,0 20,0 20,0 4 LS 0,05% 1,87 310 19,9 65,0 15,0 20,0 5 LS 0,1% 1,93 370 23,7 70,0 20,0 10,0 Fondul rigid infectat artificial, % 1 Martor (H2O) 1,02 - - 35,0 25,0 40,0 2 Nicotianozidă 0,1% 1,53 510 48,3 55,0 25,0 20,0 3 LS 0,01% 1,48 460 45,1 60,0 20,0 20,0 4 LS 0,05% 1,59 570 55,9 65,0 15,0 20,0 5 LS 0,1% 1,67 650 63,7 70,0 10,0 20,0No. d/o Variant Yield, t/ha Yield addition Quality yield, % kg/ha compared to % compared to I sort II sort III sort Control Naturally infected background, % 1 Control (H2O) 1.56 - - 55.0 20.0 25.0 2 Nicotianoside 0.1% 1.81 250 16.1 65.0 10.0 25.0 3 LS 0.01% 1.79 230 14.7 60.0 20.0 20.0 4 LS 0.05% 1.87 310 19.9 65.0 15.0 20.0 5 LS 0.1% 1.93 370 23.7 70.0 20.0 10.0 Artificially infected rigid background, % 1 Control (H2O) 1.02 - - 35.0 25.0 40.0 2 Nicothianoside 0.1% 1.53 510 48.3 55.0 25.0 20.0 3 LS 0.01% 1.48 460 45.1 60.0 20.0 20.0 4 LS 0.05% 1.59 570 55.9 65.0 15.0 20.0 5 LS 0.1% 1.67 650 63.7 70.0 10.0 20.0
Rezultatele obţinute denotă că tratarea seminţelor şi răsadurilor de tutun cu soluţie de linariozide în concentraţie de 0,1% sporeşte recolta la hectar cu 23,7% pe fondul infectat natural, iar calitatea frunzelor este mai bună comparativ cu varianta martor. Pe fondul rigid infectat artificial, în aceiaşi variantă, sporul la roadă este de 650 kg/ha, ce constituie 63,4%, comparativ cu varianta martor, iar randamentul calităţii superioare pentru sortul I rămâne neschimbat, spre deosebire de celelalte variante (tab. 3). The results obtained show that the treatment of tobacco seeds and seedlings with a 0.1% linarioside solution increases the yield per hectare by 23.7% on the naturally infected background, and the quality of the leaves is better compared to the control variant. On the artificially infected rigid background, in the same variant, the increase in yield is 650 kg/ha, which is 63.4%, compared to the control variant, and the yield of superior quality for variety I remains unchanged, unlike the other variants (tab. 3).
Rezultatele obţinute confirmă că tratarea seminţelor şi răsadurilor de tutun cu soluţie de linariozide în concentraţie de 0,1% sporeşte recolta la hectar de 1,5 ori sau cu 120 kg/ha faţă de nicotianozidă, cu 7,0% pe fondul infectat natural, iar calitatea frunzelor este mai bună comparativ cu varianta nicotianozidă. Pe fondul rigid infectat artificial, în aceeaşi variantă, sporul la roadă este de 140 kg/ha, ce constituie 27,4%, comparativ cu varianta nicotianozidă, iar randamentul calităţii superioare pentru sortul I constituie un surplus de 15%, spre deosebire de varianta nicotianozidă. The results obtained confirm that the treatment of tobacco seeds and seedlings with a 0.1% linarioside solution increases the yield per hectare by 1.5 times or by 120 kg/ha compared to nicotianozide, by 7.0% on the naturally infected background, and the quality of the leaves is better compared to the nicotianozide variant. On the rigid artificially infected background, in the same variant, the increase in yield is 140 kg/ha, which is 27.4%, compared to the nicotianozide variant, and the yield of superior quality for variety I is a surplus of 15%, unlike the nicotianozide variant.
Prin urmare, procedeul propus este tehnologic simplu, eficient şi permite utilizarea substanţelor naturale, ecologic inofensive, şi anume a linariozidelor, obţinute din planta Linaria vulgaris L. Mill (care este răspândită în flora spontană şi poate fi colectată în cantităţi mari), în agricultură în calitate de substanţe ce contribuie la creşterea energiei germinative a seminţelor, la stimularea, creşterea şi dezvoltarea răsadurilor şi a plantelor de tutun, la mărirea imunităţii tutunului faţă de putrezirea neagră a rădăcinii, concomitent sporesc recolta de tutun la hectar şi calitatea roadei. Therefore, the proposed process is technologically simple, efficient and allows the use of natural, ecologically harmless substances, namely linariosides, obtained from the Linaria vulgaris L. Mill plant (which is widespread in the spontaneous flora and can be collected in large quantities), in agriculture as substances that contribute to increasing the germination energy of seeds, to stimulating, growing and developing seedlings and tobacco plants, to increasing tobacco immunity to black root rot, while simultaneously increasing the tobacco harvest per hectare and the quality of the fruit.
1. MD 2653 F1 2005.01.31 1. MD 2653 F1 2005.01.31
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20140059A MD844Z (en) | 2014-05-07 | 2014-05-07 | Process for growing tobacco plants |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDS20140059A MD844Z (en) | 2014-05-07 | 2014-05-07 | Process for growing tobacco plants |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MD844Y MD844Y (en) | 2014-12-31 |
| MD844Z true MD844Z (en) | 2015-07-31 |
Family
ID=52211078
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MDS20140059A MD844Z (en) | 2014-05-07 | 2014-05-07 | Process for growing tobacco plants |
Country Status (1)
| Country | Link |
|---|---|
| MD (1) | MD844Z (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD1012Z (en) * | 2015-09-09 | 2016-10-31 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | Process for treatment of grape-vine |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD385C2 (en) * | 1994-12-28 | 1996-06-30 | Научно-исследовательский конструкторско-технологический институт табака и табачных изделий | Tobacco chemical immunizer to the tobacco tronzing virus |
| MD369C2 (en) * | 1994-12-28 | 1996-06-30 | Научно-исследовательский конструкторско-технологический институт табака и табачных изделий | Tobacco chemical immunizator |
| MD368C2 (en) * | 1994-12-28 | 1996-06-30 | Научно-исследовательский конструкторско-технологический институт табака и табачных изделий | Tobacco chemical immunizator |
| MD2133G2 (en) * | 2002-02-05 | 2003-10-31 | Научно-исследовательский конструкторско-технологический институт табака и табачных изделий | Process for tobacco growth stimulation |
| MD2653F1 (en) * | 2003-07-25 | 2005-01-31 | Institutul De Genetica Al Academiei De Stiinte A Republicii Moldova | Process for tobacco cultivation |
| MD2570G2 (en) * | 2003-11-11 | 2005-05-31 | Институт Генетики И Физиологии Растений Академии Наук Молдовы | Composition for presowing treatment of tobacco seeds |
| MD3866C2 (en) * | 2008-12-19 | 2009-10-31 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | 5,4-dimethylcampherol 3-O--D-(6′′-a′-L-rhamnopyranosyl)-glucopyranoside as compound increasing the seed productivity |
| MD98Y (en) * | 2009-06-19 | 2009-11-30 | Institutul De Genetica Si Fiziologie A Plantelor Al Academiei De Stiinte A Moldovei | Process for presowing treatment of sweet pepper seeds |
| MD566Y (en) * | 2012-05-07 | 2012-12-31 | Inst De Genetica Si Fiziol A Plantelor Al Academiei De Stiinte A Moldovei | Process for cultivation of apricot plants |
| MD636Y (en) * | 2012-12-21 | 2013-05-31 | Inst De Genetica Si Fiziol A Plantelor Al Academiei De Stiinte A Moldovei | Process for producing flavonoid glycosides from Linaria vulgaris Mill. |
-
2014
- 2014-05-07 MD MDS20140059A patent/MD844Z/en not_active IP Right Cessation
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MD385C2 (en) * | 1994-12-28 | 1996-06-30 | Научно-исследовательский конструкторско-технологический институт табака и табачных изделий | Tobacco chemical immunizer to the tobacco tronzing virus |
| MD369C2 (en) * | 1994-12-28 | 1996-06-30 | Научно-исследовательский конструкторско-технологический институт табака и табачных изделий | Tobacco chemical immunizator |
| MD368C2 (en) * | 1994-12-28 | 1996-06-30 | Научно-исследовательский конструкторско-технологический институт табака и табачных изделий | Tobacco chemical immunizator |
| MD2133G2 (en) * | 2002-02-05 | 2003-10-31 | Научно-исследовательский конструкторско-технологический институт табака и табачных изделий | Process for tobacco growth stimulation |
| MD2653F1 (en) * | 2003-07-25 | 2005-01-31 | Institutul De Genetica Al Academiei De Stiinte A Republicii Moldova | Process for tobacco cultivation |
| MD2570G2 (en) * | 2003-11-11 | 2005-05-31 | Институт Генетики И Физиологии Растений Академии Наук Молдовы | Composition for presowing treatment of tobacco seeds |
| MD3866C2 (en) * | 2008-12-19 | 2009-10-31 | Институт Генетики, Физиологии И Защиты Растений Академии Наук Молдовы | 5,4-dimethylcampherol 3-O--D-(6′′-a′-L-rhamnopyranosyl)-glucopyranoside as compound increasing the seed productivity |
| MD98Y (en) * | 2009-06-19 | 2009-11-30 | Institutul De Genetica Si Fiziologie A Plantelor Al Academiei De Stiinte A Moldovei | Process for presowing treatment of sweet pepper seeds |
| MD566Y (en) * | 2012-05-07 | 2012-12-31 | Inst De Genetica Si Fiziol A Plantelor Al Academiei De Stiinte A Moldovei | Process for cultivation of apricot plants |
| MD636Y (en) * | 2012-12-21 | 2013-05-31 | Inst De Genetica Si Fiziol A Plantelor Al Academiei De Stiinte A Moldovei | Process for producing flavonoid glycosides from Linaria vulgaris Mill. |
Also Published As
| Publication number | Publication date |
|---|---|
| MD844Y (en) | 2014-12-31 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Anjanawe et al. | Effect of plant growth regulators and growth media on seed germination and growth vigour of papaya | |
| CN104982232B (en) | A kind of method of tea tree Grafting kind | |
| CN104718930A (en) | High-yield and high-quality cultivation method for bitter gourds | |
| WO2015093607A1 (en) | Method for cultivating araliaceae medicinal plants | |
| CN110249894B (en) | Interplanting sweet corn in mango orchard and crop rotation cultivation method of sweet corn and sweet crisp peas | |
| CN113016513B (en) | Sesame and saffron intercropping and interplanting method | |
| CN106664877A (en) | Method for planting high-yield and high-quality peanuts | |
| Atta-Aly | Soaking summer squash seeds in low concentrations of cobalt solution before sowing increased plant growth, femaleness, and fruit yield via increasing plant ethylene level | |
| CN105794429A (en) | An eggplant planting method | |
| MD844Z (en) | Process for growing tobacco plants | |
| RU2661815C2 (en) | Method of growing chickpeas in dry conditions of the steppe crimea | |
| Makukha | Technological improvement of Echinacea purpurea cultivation | |
| KR101639552B1 (en) | Forest Ginseng Cultivated From Wild Ginseng Berry And Cultivation Method Thereof | |
| CN109089755A (en) | The implantation methods of broccoli shoot vegetable | |
| SUNUSI et al. | Impact of foliar application of moringa leaf extract on growth and yield of different cultivars of cucumber (Cucumis sativus) in Nigeria | |
| Nedunchezhiyan et al. | Polypropylene fabric ground cover effects on weed control and profit in elephant foot yam cultivation | |
| CN108260492B (en) | Film-covering hole sowing planting method for aerospace mutagenesis red clover seed field | |
| Neelavathi et al. | Propagation of acid lime, Citrus aurantifolia (Christm.) swingle and lemon, Citrus limon L. through stem cuttings-A review | |
| Glala et al. | Increasing organic production of summer squash by modulating plant sex ratio | |
| CN111955303A (en) | Flue-cured tobacco and sweet pea interplanting method | |
| Ashok et al. | Influence of NAA on root promotion in vegetative propagation of Duranta erecta L | |
| Chumthong et al. | Influence of ethephon spraying on the growth and yield of Stevia (Stevia rebaudiana Berton. | |
| SAHA | H-2020-021-M | |
| Lassa et al. | RESPONSE OF TOMATO (Lycopersicon lycopersicum L.) TO CONCENTRATION AND FREQUENCY OF APPLICATION OF MORINGA LEAF EXTRACT AT MAIDUGURI, SUDAN SAVANNA ECOLOGICAL ZONE, NIGERIA | |
| Rampe et al. | Morphological characteristics of sweet potato (Ipomoea batatas L.) after being given elicitor salicylic acid |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG9Y | Short term patent issued | ||
| KA4A | Patent for invention lapsed due to non-payment of fees (with right of restoration) |