MD1012Z - Process for treatment of grape-vine - Google Patents
Process for treatment of grape-vine Download PDFInfo
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- MD1012Z MD1012Z MDS20150124A MDS20150124A MD1012Z MD 1012 Z MD1012 Z MD 1012Z MD S20150124 A MDS20150124 A MD S20150124A MD S20150124 A MDS20150124 A MD S20150124A MD 1012 Z MD1012 Z MD 1012Z
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- grapevine
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- 238000000034 method Methods 0.000 title claims abstract description 17
- 240000006365 Vitis vinifera Species 0.000 title claims description 21
- 235000014787 Vitis vinifera Nutrition 0.000 title claims description 12
- 241000196324 Embryophyta Species 0.000 claims abstract description 38
- 239000000243 solution Substances 0.000 claims abstract description 15
- 239000007864 aqueous solution Substances 0.000 claims abstract description 11
- 241000167860 Linaria vulgaris Species 0.000 claims abstract description 5
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 claims abstract description 5
- 238000009835 boiling Methods 0.000 claims abstract description 4
- 238000013375 chromatographic separation Methods 0.000 claims abstract description 3
- 229930182487 phenolic glycoside Natural products 0.000 claims description 5
- 150000007950 phenolic glycosides Chemical class 0.000 claims description 5
- 238000000605 extraction Methods 0.000 claims description 2
- 229930182470 glycoside Natural products 0.000 abstract description 9
- 150000002338 glycosides Chemical class 0.000 abstract description 8
- 238000009369 viticulture Methods 0.000 abstract description 2
- 235000013824 polyphenols Nutrition 0.000 abstract 1
- 235000021028 berry Nutrition 0.000 description 11
- 230000035800 maturation Effects 0.000 description 9
- 235000009754 Vitis X bourquina Nutrition 0.000 description 7
- 235000012333 Vitis X labruscana Nutrition 0.000 description 7
- 239000013543 active substance Substances 0.000 description 7
- 241000219094 Vitaceae Species 0.000 description 6
- 235000021021 grapes Nutrition 0.000 description 6
- 238000003306 harvesting Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 235000000346 sugar Nutrition 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 4
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 229930002534 steroid glycoside Natural products 0.000 description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- WVDDGKGOMKODPV-UHFFFAOYSA-N hydroxymethyl benzene Natural products OCC1=CC=CC=C1 WVDDGKGOMKODPV-UHFFFAOYSA-N 0.000 description 2
- 239000000741 silica gel Substances 0.000 description 2
- 229910002027 silica gel Inorganic materials 0.000 description 2
- 239000011877 solvent mixture Substances 0.000 description 2
- 238000005292 vacuum distillation Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 238000001644 13C nuclear magnetic resonance spectroscopy Methods 0.000 description 1
- 238000005160 1H NMR spectroscopy Methods 0.000 description 1
- JNNRILAYMZYEQB-FZFRBNDOSA-N 5-hydroxy-6-methoxy-2-(4-methoxyphenyl)-7-[(2s,3r,4s,5s,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxychromen-4-one Chemical compound C1=CC(OC)=CC=C1C(OC1=C2)=CC(=O)C1=C(O)C(OC)=C2O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 JNNRILAYMZYEQB-FZFRBNDOSA-N 0.000 description 1
- JNNRILAYMZYEQB-UHFFFAOYSA-N 6,4'-dimethoxy-5-hydroxyflavone 7-glucoside Natural products C1=CC(OC)=CC=C1C(OC1=C2)=CC(=O)C1=C(O)C(OC)=C2OC1C(O)C(O)C(O)C(CO)O1 JNNRILAYMZYEQB-UHFFFAOYSA-N 0.000 description 1
- 244000178870 Lavandula angustifolia Species 0.000 description 1
- DRWIVSAUXCDFQK-UHFFFAOYSA-N Linarioside Natural products C12C(C)(O)C(Cl)C(O)C2(O)C=COC1OC1OC(CO)C(O)C(O)C1O DRWIVSAUXCDFQK-UHFFFAOYSA-N 0.000 description 1
- 239000004952 Polyamide Substances 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 235000019445 benzyl alcohol Nutrition 0.000 description 1
- -1 benzyl alcohol glycosides Chemical class 0.000 description 1
- SIHHLZPXQLFPMC-UHFFFAOYSA-N chloroform;methanol;hydrate Chemical compound O.OC.ClC(Cl)Cl SIHHLZPXQLFPMC-UHFFFAOYSA-N 0.000 description 1
- 230000001143 conditioned effect Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- GBMDVOWEEQVZKZ-UHFFFAOYSA-N methanol;hydrate Chemical compound O.OC GBMDVOWEEQVZKZ-UHFFFAOYSA-N 0.000 description 1
- 230000000116 mitigating effect Effects 0.000 description 1
- 230000017066 negative regulation of growth Effects 0.000 description 1
- 229920002647 polyamide Polymers 0.000 description 1
- 230000027272 reproductive process Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000010898 silica gel chromatography Methods 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 238000004809 thin layer chromatography Methods 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Invenţia se referă la viticultură, în special la un procedeu de tratare a viţei-de-vie.Procedeul, conform invenţiei, include tratarea extraradiculară a plantelor de viţă-de-vie cu soluţie apoasă de 0,0001…0,01% de glicozide fenolice obţinute din Linaria vulgaris L. Mill prin extragere la fierbere cu soluţie hidrometanolică şi separare cromatografică a fracţiunii cu Rf 0,54…0,69, totodată tratarea se efectuează cu 8…12 zile înainte de înflorirea în masă cu un consum total de 0,25…0,30 L/plantă sau 500…600 L/ha.The invention relates to viticulture, in particular to a process for treating vines. The process according to the invention includes extraradicular treatment of vine plants with aqueous solution of 0.0001 ... 0.01% glycosides phenolics obtained from Linaria vulgaris L. Mill by boiling with hydromethanolic solution and chromatographic separation of the fraction with Rf 0.54 ... 0.69, at the same time the treatment is carried out 8 ... 12 days before mass flowering with a total consumption of 0 , 25 ... 0,30 L / plant or 500 ... 600 L / ha.
Description
Invenţia se referă la viticultură, în special la un procedeu de tratare a viţei-de-vie şi poate fi aplicată la cultivarea viţei-de-vie în scopul sporirii recoltei şi calităţii ei. The invention relates to viticulture, in particular to a method of treating vines and can be applied to vine cultivation in order to increase the harvest and its quality.
Cea mai apropriată soluţie de procedeul revendicat reprezintă procedeul de tratare extraradiculară a plantelor de viţă-de-vie cu 8...12 zile până la înflorirea în masă cu soluţie apoasă de glicozidă steroidică 3-O-[β-D-glucopiranozil(1→2)-β-D-glucopiranozil]-26-O(β-D-glucopiranozil)-(25R)-furost-5-en-3ß,22α,26-triol (melangozidă O) în concentraţie de 0,001...0,010% cu un consum total de 500…600 L/ha [1]. The most appropriate solution to the claimed process is the process of extra-radicular treatment of grapevine plants 8...12 days before mass flowering with an aqueous solution of the steroid glycoside 3-O-[β-D-glucopyranosyl(1→2)-β-D-glucopyranosyl]-26-O(β-D-glucopyranosyl)-(25R)-furost-5-en-3ß,22α,26-triol (melanoside O) in a concentration of 0.001...0.010% with a total consumption of 500...600 L/ha [1].
Dezavantajul acestui procedeu constă în eficacitatea diminuată, în special în condiţii nefavorabile pe parcursul perioadei de vegetaţie. The disadvantage of this process is its reduced effectiveness, especially in unfavorable conditions during the growing season.
Problema pe care o rezolvă invenţia constă în optimizarea creşterii, sporirea recoltei şi calităţii ei, precum şi în atenuarea acţiunii factorilor nefavorabili de mediu (secetă, arşiţă) asupra proceselor generative şi de creştere a plantelor de viţă-de-vie. The problem solved by the invention consists in optimizing growth, increasing the harvest and its quality, as well as mitigating the action of unfavorable environmental factors (drought, heat) on the generative and growth processes of grapevine plants.
Procedeul, conform invenţiei, include tratarea extraradiculară a plantelor de viţă-de-vie cu soluţie apoasă de 0,0001…0,01% de glicozide fenolice obţinute din Linaria vulgaris L. Mill prin extragere la fierbere cu soluţie hidrometanolică şi separare cromatografică a fracţiunii cu Rf 0,54…0,69, totodată tratarea se efectuează cu 8…12 zile înainte de înflorirea în masă cu un consum total de 0,25…0,30 L/plantă sau 500…600 L/ha. The process, according to the invention, includes extra-radicular treatment of grapevine plants with an aqueous solution of 0.0001…0.01% of phenolic glycosides obtained from Linaria vulgaris L. Mill by boiling extraction with hydromethanolic solution and chromatographic separation of the fraction with Rf 0.54…0.69, at the same time the treatment is carried out 8…12 days before mass flowering with a total consumption of 0.25…0.30 L/plant or 500…600 L/ha.
Glicozidele fenolice, linarozidele sumare (LS) din L.vulgaris au fost obţinute în modul următor: masa vegetală de plantă proaspătă (0,5 kg) a fost mărunţită şi extrasă prin fierbere cu soluţie hidrometanolică de 60%, în trei repetări. Extractele au fost unite, concentrate prin distilare în vid, iar reziduul apos a fost decantat cu benzen pentru înlăturarea balastului. Restul apos a fost concentrat în vid şi aplicat pe coloana cu poliamidă. Coloana a fost eluată cu apă, apoi cu amestecul de solvenţi apă-metanol (4:1 vol/vol). Au fost colectate porţiuni a câte 10 ml, care au fost analizate în strat subţire de silicagel în amestecul de solvenţi cloroform-metanol-apă (76:14:3 vol/vol/vol) şi unite conform constantelor cromatografice. În acest mod s-au obţinut mai multe fracţiuni îmbogăţite cu substanţe biologic active. Fracţiunea cu un conţinut predominant de glicozide cu valorile constantelor cromatografice Rf cuprinse între 0,54…0,69 a fost concentrată prin distilare în vid până la uscat. În reziduul uscat al fracţiunii respective au fost detectate cu ajutorul cromatografiei în strat subţire de silicagel 3 glicozide majore, structura cărora a fost stabilită după separare prin cromatografiere pe coloane cu silicagel cu ajutorul procedeelor fizico-chimice, spectroscopiei 1H şi 13C RMN. Astfel, în extractul sumar au fost identificate o glicozidă nouă, denumită de autori linariozida V, şi două glicozide ale alcoolului benzilic (Natalia Mashcenco, Pavel Kintea, Angela Gurev ş.a. Glycosides from Linaria vulgaris Mill. Chemistry Journal of Moldova, v.3, nr 2, 2008; p.98-100). Phenolic glycosides, total linarosides (LS) from L. vulgaris were obtained in the following way: the fresh plant mass (0.5 kg) was ground and extracted by boiling with 60% hydromethanol solution, in three repetitions. The extracts were combined, concentrated by vacuum distillation, and the aqueous residue was decanted with benzene to remove the ballast. The aqueous residue was concentrated in vacuum and applied to the polyamide column. The column was eluted with water, then with the water-methanol solvent mixture (4:1 vol/vol). Portions of 10 ml each were collected, which were analyzed in a thin layer of silica gel in the chloroform-methanol-water solvent mixture (76:14:3 vol/vol/vol) and combined according to the chromatographic constants. In this way, several fractions enriched with biologically active substances were obtained. The fraction with a predominant content of glycosides with the values of the chromatographic constants Rf ranging from 0.54…0.69 was concentrated by vacuum distillation to dryness. In the dry residue of the respective fraction, 3 major glycosides were detected using silica gel thin layer chromatography, the structure of which was established after separation by silica gel column chromatography using physicochemical procedures, 1H and 13C NMR spectroscopy. Thus, in the summary extract, a new glycoside, called by the authors linarioside V, and two benzyl alcohol glycosides were identified (Natalia Mashcenco, Pavel Kintea, Angela Gurev et al. Glycosides from Linaria vulgaris Mill. Chemistry Journal of Moldova, v.3, no. 2, 2008; p.98-100).
Rezultatul tehnic al invenţiei constă în optimizarea creşterii lăstarilor şi maturării ţesuturilor lor, sporirea recoltei şi a calităţii ei în baza formării şi dezvoltării normale a unui număr mai mare de ciorchini, acumulării mai sporite a asimilatelor în boabe, ameliorarea coraportului dintre conţinutul de zahăr şi acizi în ele. The technical result of the invention consists in optimizing the growth of shoots and the maturation of their tissues, increasing the harvest and its quality based on the formation and normal development of a greater number of clusters, increased accumulation of assimilates in the berries, and improving the ratio between the sugar and acid content in them.
Exemplu de realizare a invenţiei Example of embodiment of the invention
Experienţele au fost efectuate în condiţii de câmp, în viile colegiului viti-vinicol Stăuceni, în anii 2013-2014. În calitate de obiect de studiu au servit plantele de viţă-de-vie, soiul Aligote. Cu 8...12 zile până la înflorirea în masă plantele au fost tratate cu soluţii apoase de substanţe biologic active după următoarea schemă: un lot de plante au fost tratate extraradicular (stropite) cu soluţii apoase ale sumei linarozidelor (LS), ce reprezintă glicozide fenolice de origine vegetală, în concentraţii de 0,0001, 0,001, 0,005 şi 0,01% (invenţia propusă), fiind organizate variante corespunzătoare; alt lot de plante au fost tratate cu soluţie apoasă de glicozidă steroidică melangozidă O în concentraţie de 0,001% (cea mai apropiată soluţie); în calitate de martor au servit plantele stropite cu apă. Experienţa a fost montată pe teren cu amplasarea randomizată a variantelor în blocuri, în 3 repetări, câte 15 plante în fiecare repetare a variantei respective. Consumul de soluţie a constituit 0,25…0,3 L/plantă, sau 500…600 L/ha. The experiments were carried out in field conditions, in the vineyards of the Stăuceni Viticultural College, in 2013-2014. The Aligote grapevine plants served as the object of study. 8...12 days before mass flowering, the plants were treated with aqueous solutions of biologically active substances according to the following scheme: one batch of plants was treated extraradicularly (sprayed) with aqueous solutions of the sum of linarosides (LS), which represent phenolic glycosides of plant origin, in concentrations of 0.0001, 0.001, 0.005 and 0.01% (the proposed invention), corresponding variants being organized; another batch of plants was treated with an aqueous solution of the steroid glycoside melangoside O in a concentration of 0.001% (the closest solution); the plants sprayed with water served as the control. The experiment was set up in the field with randomized block placement of the variants, in 3 repetitions, 15 plants in each repetition of the respective variant. The solution consumption was 0.25…0.3 L/plant, or 500…600 L/ha.
Pentru relevarea reacţiei plantelor la tratamentul cu substanţele biologic active în studiu au fost determinaţi următorii indici: creşterea lăstarilor şi gradul de maturare a ţesuturilor lor, recolta de pe un butuc, numărul de struguri per plantă, masa unui strugure, masa a 100 de boabe, volumul a 100 de boabe, conţinutul de zaharuri şi aciditatea boabelor. Rezultatele obţinute sunt prezentate în tabelele 1-3. To reveal the reaction of plants to the treatment with biologically active substances under study, the following indices were determined: shoot growth and the degree of maturation of their tissues, the harvest from a stump, the number of grapes per plant, the mass of a grape, the mass of 100 berries, the volume of 100 berries, the sugar content and acidity of the berries. The results obtained are presented in tables 1-3.
Datele prezentate în tabelele 1 şi 2 scot în evidenţă o influenţă diferită a substanţelor biologic active incluse în studiu asupra vigorii de creştere a lăstarilor plantelor de viţă-de-vie în raport cu martorul. În condiţiile climatice ale perioadei de vegetaţie activă din anii 2013 şi 2014, mai puţin favorabile pentru creşterea şi dezvoltarea plantelor de viţă-de-vie, aplicarea glicozidei steroidice, şi anume a preparatului melangozidă O (cea mai apropiată soluţie), exercită o acţiune slab pronunţată de inhibare asupra proceselor de creştere a lăstarilor, faţă de plantele martor, în anul 2013 şi, practic, nu influenţează vigoarea de creştere a lăstarilor în anul 2014. La tratarea plantelor cu soluţii apoase de linarozide (LS) (invenţia propusă) acţiunea asupra proceselor de creştere a lăstarilor şi de maturare a ţesuturilor lor se manifestă în funcţie de concentraţia aplicată, precum şi de condiţiile climatice ale anului. Aprecierea influenţei SBA în studiu asupra vigorii de creştere a lăstarilor s-a efectuat în baza determinării sporului lungimii lăstarilor în % faţă de lungimea la momentul tratării plantelor; estimarea gradului de maturare a ţesuturilor lăstarilor - prin exprimarea părţii maturate în procente din lungimea totală a lăstarilor la sfârşitul vegetaţiei. The data presented in Tables 1 and 2 highlight a different influence of the biologically active substances included in the study on the growth vigor of grapevine plant shoots compared to the control. In the climatic conditions of the active vegetation period in 2013 and 2014, less favorable for the growth and development of grapevine plants, the application of the steroid glycoside, namely the melanoside O preparation (the closest solution), exerts a weakly pronounced inhibitory action on the shoot growth processes, compared to the control plants, in 2013 and, practically, does not influence the shoot growth vigor in 2014. When treating plants with aqueous solutions of linaroside (LS) (the proposed invention) the action on the shoot growth processes and maturation of their tissues manifests itself depending on the applied concentration, as well as the climatic conditions of the year. The assessment of the influence of SBA in the study on the shoot growth vigor was carried out based on determining the increase in shoot length in % compared to the length at the time of plant treatment; estimating the degree of maturation of shoot tissues - by expressing the matured part as a percentage of the total shoot length at the end of vegetation.
Conform datelor experimentale, obţinute în anul 2013 (tab.1), la plantele tratate cu soluţii apoase ale sumei linarozidelor (LS) (invenţia propusă) în concentraţii de 0,0001, 0,001, 0,005, 0,01% sporul lungimii lăstarilor pe parcursul perioadei de vegetaţie faţă de lungimea lor la momentul tratării a constituit 34,9; 40,7; 46,2; 53,7% respectiv, la plantele tratate cu melangozidă O în concentraţia de 0,001% (cea mai apropiată soluţie) - 50,0%, iar la plantele din varianta martor - 78,5%. În condiţiile acestui an s-a evidenţiat elocvent activitatea LS de inhibare a proceselor de creştere a lăstarilor, acest efect fiind mai pronunţat la aplicarea linarozidelor în concentraţii mai mici. Însă inhibarea proceselor de creştere a favorizat într-o oarecare măsură maturarea ţesuturilor lăstarilor, gradul căreia s-a dovedit a fi mai sporit la plantele tratate cu LS în concentraţii de 0,001% şi 0,0001% ( tab.1) - 27,1% şi 25,6% respectiv, pe când la plantele tratate cu melangozidă O acest indice a constituit 20,5%, iar la plantele din varianta martor 19,9%. According to experimental data obtained in 2013 (tab. 1), in plants treated with aqueous solutions of the sum of linarosides (LS) (the proposed invention) in concentrations of 0.0001, 0.001, 0.005, 0.01%, the increase in shoot length during the vegetation period compared to their length at the time of treatment was 34.9; 40.7; 46.2; 53.7%, respectively, in plants treated with melanoside O in the concentration of 0.001% (the closest solution) - 50.0%, and in plants in the control variant - 78.5%. Under the conditions of this year, the activity of LS to inhibit shoot growth processes was eloquently highlighted, this effect being more pronounced when applying linarosides in lower concentrations. However, the inhibition of growth processes favored to some extent the maturation of shoot tissues, the degree of which proved to be higher in plants treated with LS in concentrations of 0.001% and 0.0001% (tab. 1) - 27.1% and 25.6% respectively, while in plants treated with melanoside O this index was 20.5%, and in plants in the control variant 19.9%.
Tabelul 1 Table 1
Creşterea lăstarilor şi gradul de maturare a ţesuturilor lor la viţa-de-vie tratată cu substanţe biologic active (glicozide), s. Aligote, a. 2013 Shoot growth and degree of maturation of their tissues in grapevines treated with biologically active substances (glycosides), Aligote village, 2013
Varianta Lungimea lăstarului, cm Sporul lungimii faţă de lungimea iniţială B/A, % Partea maturată A, la tratare B, la sfârşitul vegetaţiei C, cm C/B, % 1.Martor 56,3 ± 5,9 100,5 ± 8,3 78,5 20,0 ± 4,5 19,9 2. LS 0,0001% 59,1 ± 1,3 79,7 ± 1,1 34,9 20,4 ± 3,7 25,6 3. LS 0,001% 68,1 ± 6,0 95,8 ± 3,2 40,7 26,0 ± 3,6 27,1 4. LS 0,005% 61,0 ± 2,1 89,2 ± 0,9 46,2 16,4 ± 2,3 18,4 5. LS 0,01% 50,5 ± 4,9 77,6 ± 7,5 53,7 18,0 ± 1,0 23,2 6. Melangozidă O 0,001% 52,0 ± 2,2 78,0 ± 2,6 50,0 16,0 ± 0,90 20,5Variant Shoot length, cm Length increase compared to initial length B/A, % Matured part A, at treatment B, at the end of vegetation C, cm C/B, % 1.Control 56.3 ± 5.9 100.5 ± 8.3 78.5 20.0 ± 4.5 19.9 2. LS 0.0001% 59.1 ± 1.3 79.7 ± 1.1 34.9 20.4 ± 3.7 25.6 3. LS 0.001% 68.1 ± 6.0 95.8 ± 3.2 40.7 26.0 ± 3.6 27.1 4. LS 0.005% 61.0 ± 2.1 89.2 ± 0.9 46.2 16.4 ± 2.3 18.4 5. LS 0.01% 50.5 ± 4.9 77.6 ± 7.5 53.7 18.0 ± 1.0 23.2 6. Melangoside O 0.001% 52.0 ± 2.2 78.0 ± 2.6 50.0 16.0 ± 0.90 20.5
În condiţiile anului 2014 tratarea plantelor cu LS practic n-a influenţat vigoarea de creştere a lăstarilor, cu excepţia concentraţiei 0,005%, aplicarea căreia a produs un efect slab pronunţat de stimulare a acestor procese (tab.2). Ca şi în anul precedent, un grad mai înalt de maturare a ţesururilor lăstarilor, 18,3% şi 16,5%, s-a înregistrat la plantele tratate cu LS în concentraţiile 0,001% şi 0,0001% respectiv; în varianta melangozidă O 0,001% (cea mai apropiată soluţie) acest indice a constituit 16,6% şi la plantele din varianta martor - 15,5%. In the conditions of 2014, the treatment of plants with LS practically did not influence the growth vigor of shoots, except for the concentration of 0.005%, the application of which produced a weakly pronounced effect of stimulating these processes (tab. 2). As in the previous year, a higher degree of maturation of shoot tissues, 18.3% and 16.5%, was recorded in plants treated with LS in concentrations of 0.001% and 0.0001%, respectively; in the 0.001% melanoside O variant (the closest solution) this index constituted 16.6% and in plants in the control variant - 15.5%.
Tabelul 2 Table 2
Creşterea lăstarilor şi gradul de maturare a ţesuturilor lor la viţa-de-vie tratată cu substanţe biologic active (glicozide), s. Aligote. a. 2014 Shoot growth and the degree of maturation of their tissues in grapevines treated with biologically active substances (glycosides), village Aligote. 2014
Varianta Lungimea lăstarului, cm Sporul lungimii faţă de lungimea iniţială B/A, % Partea maturată A, la tratare B, la sfârşitul vegetaţiei C, cm C/B, % 1.Martor 52,9 ± 7,1 99,3 ± 5,87 87,7 15,4 ± 0,71 15,5 2. LS 0,0001% 50,2 ± 2,6 92,4 ± 4,6 84,1 15,2 ± 1,97 16,5 3. LS 0,001% 51,2 ± 6,0 88,2 ± 11,3 72,3 16,1 ± 1,62 18,3 4. LS 0,005% 52,8 ± 2,9 111,8 ± 4,1 111,7 15,1 ± 1,98 13,5 5. LS 0,01% 55,6 ± 3,3 103,2 ± 15,9 85,6 15,3 ± 2,83 14,8 6. Melangozidă O 0,001% 49,9 ± 1,98 95,8 ± 11,2 91,9 15,9 ± 2,04 16,6Variant Shoot length, cm Length increase compared to initial length B/A, % Matured part A, at treatment B, at the end of vegetation C, cm C/B, % 1.Control 52.9 ± 7.1 99.3 ± 5.87 87.7 15.4 ± 0.71 15.5 2. LS 0.0001% 50.2 ± 2.6 92.4 ± 4.6 84.1 15.2 ± 1.97 16.5 3. LS 0.001% 51.2 ± 6.0 88.2 ± 11.3 72.3 16.1 ± 1.62 18.3 4. LS 0.005% 52.8 ± 2.9 111.8 ± 4.1 111.7 15.1 ± 1.98 13.5 5. LS 0.01% 55.6 ± 3.3 103.2 ± 15.9 85.6 15.3 ± 2.83 14.8 6. Melangoside O 0.001% 49.9 ± 1.98 95.8 ± 11.2 91.9 15.9 ± 2.04 16.6
Datele experimentale obţinute denotă că aplicarea preparatului propus contribuie la optimizarea coraportului proceselor de creştere şi celor reproductive, asigurând manifestarea optimală a potenţialului productiv şi calitativ al plantelor. The experimental data obtained show that the application of the proposed preparation contributes to optimizing the correlation of growth and reproductive processes, ensuring the optimal manifestation of the productive and qualitative potential of plants.
Pe fondalul optimizării proceselor de creştere şi maturare a ţesuturilor lăstarilor, recolta (kg de pe un butuc) a constituit în varianta martor - 4,63; la plantele tratate conform celei mai apropiate soluţii - 5,0, iar la plantele tratate cu preparatul propus (LS) - de la 4,73 până la 6,2 kg (tab. 3), fiind mai ridicată, în special în variantele LS 0,001 şi 0,01%, cu 33,9 şi 20,9% în raport cu plantele martor şi cu 24,0 şi 12% în raport cu plantele tratate conform celei mai apropiate soluţii. Against the background of optimizing the processes of growth and maturation of shoot tissues, the harvest (kg from a stump) was in the control variant - 4.63; in plants treated according to the closest solution - 5.0, and in plants treated with the proposed preparation (LS) - from 4.73 to 6.2 kg (tab. 3), being higher, especially in the LS variants 0.001 and 0.01%, by 33.9 and 20.9% compared to the control plants and by 24.0 and 12% compared to plants treated according to the closest solution.
Datele prezentate în tabelul 3 demonstrează că sporul recoltei plantelor de viţă-de-vie tratate cu soluţii apoase ale sumei linarozidelor, în raport cu plantele martor şi cu cele tratate conform celei mai apropiate soluţii, este condiţionat atât de formarea şi dezvoltarea unui număr mai mare de struguri pe un butuc, cât şi de sporirea masei unui strugure, fiind păstrat aspectul calitativ - mărimea (volumul) boabelor. Cota parte de influenţă a sporirii numărului de struguri sau a masei unui strugure depinde de concentraţia preparatului, efectul maximal fiind obţinut la îmbinarea optimală a acestor doi factori, care s-a manifestat la aplicarea soluţiei apoase a sumei linarozidelor în concentraţia de 0,001%. Numărul de struguri pe un butuc la martor a constituit 52, la plantele tratate cu melangozidă O - 52, la plantele tratate cu LS în concentraţii de 0,0001%; 0,001%; 0,005%; 0,01% - 46, 56, 49, 46 respectiv. The data presented in Table 3 demonstrate that the increase in the yield of grapevine plants treated with aqueous solutions of the sum of linarosides, in relation to the control plants and those treated according to the closest solution, is conditioned both by the formation and development of a larger number of grapes on a stem, and by the increase in the mass of a grape, while the qualitative aspect - the size (volume) of the berries - is preserved. The share of influence of the increase in the number of grapes or the mass of a grape depends on the concentration of the preparation, the maximum effect being obtained at the optimal combination of these two factors, which was manifested when applying the aqueous solution of the sum of linarosides in the concentration of 0.001%. The number of grapes on a stem in the control was 52, in plants treated with melanoside O - 52, in plants treated with LS in concentrations of 0.0001%; 0.001%; 0.005%; 0.01% - 46, 56, 49, 46 respectively.
Tabelul 3 Table 3
Efectul tratării cu substanţe biologic active (glicozide) The effect of treatment with biologically active substances (glycosides)
asupra productivităţii şi calităţii roadei viţei-de-vie s. Aligote, a. 2013 on the productivity and quality of grapevine fruit in Aligote village, 2013
Varianta Recolta per butuc, (kg) Numărul de struguri per butuc Masa medie a unui strugure, g Masa medie a 100 boabe, g Volumul mediu a 100 boabe, cm3 Zahari-tatea boabelor, % Aciditatea boabelor, g/l 1. Martor 4,63 ± 0,54 52 ± 1,78 89,0 ± 11,71 184,1 ± 6,2 146,7 ± 7,6 19,2 ± 0,95 7,8 ± 0,34 2. LS 0,0001% 0,00010,0001% 4,80 ± 0,62 46 ± 3,77 104,35 ± 10,3 161,6 ± 8,56 130,7 ± 3,5 20,6 ± 0,27 7,2 ± 0,42 3. LS 0,001% 6,2 ± 0,58 56 ± 2,92 110,71 ± 4,66 183,2 ± 2,4 147,0 ± 3,3 20,2 ± 0,46 6,9 ± 0,24 4. LS 0,005% 4,73 ± 1,04 49 ± 9,3 96,53 ± 3,21 186,6 ± 16,1 156,0 ± 3,8 20,5 ± 0,47 7,1 ± 0,18 5. LS 0,01% 5,6 ± 0,69 46 ± 3,86 121,74 ± 7,6 158,9 ± 6,73 124,0 ± 3,5 20,0 ± 1,53 7,4 ± 0,66 6. Melangozidă O 0,001% 5,0 ± 0,89 52 ± 8,21 96,15 ± 4,1 172,4 ± 1,2 140,3 ± 6,3 19,6 ± 0,56 7,2 ± 0,12Variant Harvest per bush, (kg) Number of grapes per bush Average mass of a grape, g Average mass of 100 berries, g Average volume of 100 berries, cm3 Sugar content of berries, % Acidity of berries, g/l 1. Control 4.63 ± 0.54 52 ± 1.78 89.0 ± 11.71 184.1 ± 6.2 146.7 ± 7.6 19.2 ± 0.95 7.8 ± 0.34 2. LS 0.0001% 0.00010.0001% 4.80 ± 0.62 46 ± 3.77 104.35 ± 10.3 161.6 ± 8.56 130.7 ± 3.5 20.6 ± 0.27 7.2 ± 0.42 3. LS 0.001% 6.2 ± 0.58 56 ± 2.92 110.71 ± 4.66 183.2 ± 2.4 147.0 ± 3.3 20.2 ± 0.46 6.9 ± 0.24 4. LS 0.005% 4.73 ± 1.04 49 ± 9.3 96.53 ± 3.21 186.6 ± 16.1 156.0 ± 3.8 20.5 ± 0.47 7.1 ± 0.18 5. LS 0.01% 5.6 ± 0.69 46 ± 3.86 121.74 ± 7.6 158.9 ± 6.73 124.0 ± 3.5 20.0 ± 1.53 7.4 ± 0.66 6. Melangoside O 0.001% 5.0 ± 0.89 52 ± 8.21 96.15 ± 4.1 172.4 ± 1.2 140.3 ± 6.3 19.6 ± 0.56 7.2 ± 0.12
Luând în consideraţie valorile masei unui strugure se evidenţiază că sporul recoltei plantelor tratate cu LS în concetraţiile de 0,0001%; 0,005%; 0,01%, precum şi celor tratate conform celei mai apropiate soluţii, în raport cu martorul, a fost asigurat preponderent prin sporirea masei unui strugure pe fondalul formării unui numar mai scăzut sau egal de struguri, însă cu menţinerea la nivel înalt a calităţii. Conţinutul de zahăr în boabe la plantele tratate cu LS în concentraţii de 0,0001…0,01% sporeşte cu 0,8…1,4% în comparaţie cu plantele martor şi cu 0,4…1,0% faţă de plantele tratate conform celei mai apropiate soluţii, iar aciditatea se diminuează cu 7,7…16,7% şi 1,4…4,2% respectiv. Taking into account the values of the mass of a grape, it is evident that the increase in the yield of plants treated with LS in concentrations of 0.0001%; 0.005%; 0.01%, as well as those treated according to the closest solution, in relation to the control, was ensured mainly by increasing the mass of a grape against the background of the formation of a lower or equal number of grapes, but with maintaining a high level of quality. The sugar content in berries in plants treated with LS in concentrations of 0.0001…0.01% increases by 0.8…1.4% compared to control plants and by 0.4…1.0% compared to plants treated according to the closest solution, and the acidity decreases by 7.7…16.7% and 1.4…4.2% respectively.
Datele experimentale prezentate în tabele denotă impactul pozitiv, inclusiv în condiţii nefavorabile de mediu, a sumei linarozidelor (glicozide fenolice obţinute din Linaria vulgaris L. Mill) în concentraţii de 0,001...0,01% asupra creşterii şi productivităţii plantelor de viţă-de-vie cu menţinerea la nivel înalt a indicilor calităţii roadei - masa unui strugure, masa şi volumul boabelor, precum şi ameliorarea coraportului dintre conţinutul în zaharuri şi de acizi în ele. The experimental data presented in the tables denote the positive impact, including under unfavorable environmental conditions, of the sum of linarosides (phenolic glycosides obtained from Linaria vulgaris L. Mill) in concentrations of 0.001...0.01% on the growth and productivity of grapevine plants with maintaining high levels of fruit quality indices - the mass of a grape, the mass and volume of berries, as well as improving the ratio between the content of sugars and acids in them.
1. MD 3138 F1 2006.09.30 1. MD 3138 F1 2006.09.30
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| MD98Y (en) * | 2009-06-19 | 2009-11-30 | Institutul De Genetica Si Fiziologie A Plantelor Al Academiei De Stiinte A Moldovei | Process for presowing treatment of sweet pepper seeds |
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| MD844Y (en) * | 2014-05-07 | 2014-12-31 | Institutul De Genetică, Fiziologie Şi Protecţie A Plantelor Al Academiei De Ştiinţe A Moldovei | Process for growing tobacco plants |
| MD893Y (en) * | 2014-10-08 | 2015-04-30 | Institutul De Genetică, Fiziologie Şi Protecţie A Plantelor Al Academiei De Ştiinţe A Moldovei | Process for presowing treatment of onion seeds |
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|---|---|---|---|---|
| MD701F1 (en) * | 1996-09-20 | 1997-04-30 | Inst De Fiziol A Plantelor Al | Process for cultivation of the grape- vine |
| MD1089G2 (en) * | 1997-05-07 | 1999-05-31 | Институт Генетики И Физиологии Растений Академии Наук Молдовы | Vine cultivation process |
| MD2026F1 (en) * | 2001-12-05 | 2002-11-30 | Inst De Fiziol A Plantelor Al | Process for increasing the vine crop resistance to drought |
| MD2924F1 (en) * | 2005-04-06 | 2005-12-31 | Institutul De Fiziologie A Plantelor Al Academiei De Stiinte A Republicii Moldova | process for vine treatment |
| MD3138F1 (en) * | 2006-02-20 | 2006-09-29 | Institutul De Fiziologie A Plantelor Al Academieide Stiinte A Republicii Moldova | Process for vine cultivation |
| MD98Y (en) * | 2009-06-19 | 2009-11-30 | Institutul De Genetica Si Fiziologie A Plantelor Al Academiei De Stiinte A Moldovei | Process for presowing treatment of sweet pepper seeds |
| MD235Y (en) * | 2010-05-04 | 2010-07-30 | Institutul De Genetica Si Fiziologie A Plantelor Al Academiei De Stiinte A Moldovei | Process for the cultivation of vine |
| MD566Y (en) * | 2012-05-07 | 2012-12-31 | Inst De Genetica Si Fiziol A Plantelor Al Academiei De Stiinte A Moldovei | Process for cultivation of apricot plants |
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| MD893Y (en) * | 2014-10-08 | 2015-04-30 | Institutul De Genetică, Fiziologie Şi Protecţie A Plantelor Al Academiei De Ştiinţe A Moldovei | Process for presowing treatment of onion seeds |
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