MD4367C1 - Bis[N'-(2-hydroxy-kO-3-carboxybenzilidene)pyridine-3-carbohydrazidate(-1)-k2N',O]iron(III) perchlorate - water (4/5) and process for cultivation of microalga Porphyridium cruentum with its use - Google Patents
Bis[N'-(2-hydroxy-kO-3-carboxybenzilidene)pyridine-3-carbohydrazidate(-1)-k2N',O]iron(III) perchlorate - water (4/5) and process for cultivation of microalga Porphyridium cruentum with its useInfo
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- MD4367C1 MD4367C1 MDA20140067A MD20140067A MD4367C1 MD 4367 C1 MD4367 C1 MD 4367C1 MD A20140067 A MDA20140067 A MD A20140067A MD 20140067 A MD20140067 A MD 20140067A MD 4367 C1 MD4367 C1 MD 4367C1
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Abstract
Description
Invenţia se referă la chimie şi biotehnologie, în special la sinteza unui nou compus coordinativ al fierului(III) cu proprietăţi antioxidante, care poate fi utilizat în industria alimentară şi în medicină, şi la un procedeu de cultivare a microalgei Porphyridium cruentum cu utilizarea acestuia. The invention relates to chemistry and biotechnology, in particular to the synthesis of a new coordination compound of iron(III) with antioxidant properties, which can be used in the food industry and in medicine, and to a method of cultivating the microalgae Porphyridium cruentum with its use.
Este cunoscut procedeul de cultivare a microalgei Porphyridium cruentum pe mediul mineral nutritiv ce conţine, g/L: NaNO3 - 5,0; NaCl - 7,0; KCl - 7,5; MgSO4·7H2O - 1,8; Ca(NO3)2·4H2O - 0,15; KBr - 0,05; KI - 0,05; K2HPO4 - 0,2; ZnSO4·5H2O - 0,00002; CuSO4·5H2O - 0,00005; MnSO4·5H2O - 0,0003; H3BO3 - 0,0006; MoO3 - 0,00002; NaVO3 - 0,00005 şi apă distilată până la 1L; pH-ul 6,8…7,2, temperatura de 23…25°C, iluminarea de 2000…3000 lx/cm2, cu agitare lentă periodică. În prima zi de cultivare, mediul este suplimentat cu compusul [Fe3O(Gly)6(H2O)3](NO3)7·3,5H2O în concentraţie de 0,01 g/L. Conţinutul fenolilor în biomasa de Porphyridium constituie 10,6…11,8 echivalent mg acid galic /g biomasă [1]. Neajunsul acestui compus şi al procedeului utilizat constă în conţinutul redus de fenoli în biomasa de Porphyridium. The process of cultivating the microalga Porphyridium cruentum on the nutrient mineral medium containing, g/L: NaNO3 - 5.0 is known; NaCl - 7.0; KCl - 7.5; MgSO4·7H2O - 1.8; Ca(NO3)2·4H2O - 0.15; KBr - 0.05; KI - 0.05; K2HPO4 - 0.2; ZnSO4·5H2O - 0.00002; CuSO4·5H2O - 0.00005; MnSO4·5H2O - 0.0003; H3BO3 - 0.0006; MoO3 - 0.00002; NaVO3 - 0.00005 and distilled water up to 1L; pH 6.8...7.2, temperature 23...25°C, illumination 2000...3000 lx/cm2, with periodic slow stirring. On the first day of cultivation, the medium is supplemented with the compound [Fe3O(Gly)6(H2O)3](NO3)7·3.5H2O in a concentration of 0.01 g/L. The content of phenols in Porphyridium biomass is 10.6...11.8 mg gallic acid equivalent /g biomass [1]. The shortcoming of this compound and the process used is the low content of phenols in Porphyridium biomass.
Problema pe care o rezolvă prezenta invenţie constă în obţinerea unui compus nou şi elaborarea unui procedeu eficient şi reproductibil de sporire a conţinutului de fenoli în biomasa de Porphyridium cruentum CNMN-AR-01. The problem that the present invention solves consists in obtaining a new compound and developing an efficient and reproducible process for increasing the phenol content in the biomass of Porphyridium cruentum CNMN-AR-01.
Esenţa invenţiei constă în faptul că se propune un compus nou - bis[N'-(2-hidroxi-kO-3-carboxibenziliden)piridin-3-carbohidrazidat(-1)-k2N',O]fier(III) perclorat - apă (4/5), care sporeşte conţinutul de fenoli în biomasa de Porphyridium cruentum. The essence of the invention consists in the fact that a new compound is proposed - bis[N'-(2-hydroxy-kO-3-carboxybenzylidene)pyridine-3-carbohydrazidate(-1)-k2N',O]iron(III) perchlorate - water (4/5), which increases the content of phenols in the biomass of Porphyridium cruentum.
De asemenea, se propune un procedeu de cultivare a microalgei Porphyridium cruentum, care constă în aceea că se cultivă microalga pe un mediu nutritiv ce conţine, g/L: NaNO3 - 5,0; NaCl - 7,0; KCl - 7,5; MgSO4·7H2O - 1,8; Ca(NO3)2·4H2O - 0,15; KBr - 0,05; KI - 0,05; K2HPO4 - 0,2; ZnSO4·5H2O - 0,00002; CuSO4·5H2O - 0,00005; MnSO4·5H2O - 0,0003; H3BO3 - 0,0006; MoO3 - 0,00002; NaVO3 - 0,00005 şi apă distilată până la 1L, având pH-ul 6,8…7,2, la temperatura de 23…25°C şi iluminarea de 2000…3000 lx/cm2, totodată mediul conţine suplimentar compusul bis[N'-(2-hidroxi-kO-3-carboxibenziliden)piridin-3-carbohidrazidat(-1)-k2N',O]fier(III) perclorat - apă (4/5) în concentraţie de 0,009…0,01 g/L. Also, a procedure for cultivating the microalgae Porphyridium cruentum is proposed, which consists in cultivating the microalgae on a nutrient medium containing, g/L: NaNO3 - 5.0; NaCl - 7.0; KCl - 7.5; MgSO4·7H2O - 1.8; Ca(NO3)2·4H2O - 0.15; KBr - 0.05; KI - 0.05; K2HPO4 - 0.2; ZnSO4·5H2O - 0.00002; CuSO4·5H2O - 0.00005; MnSO4·5H2O - 0.0003; H3BO3 - 0.0006; MoO3 - 0.00002; NaVO3 - 0.00005 and distilled water up to 1L, having a pH of 6.8...7.2, at a temperature of 23...25°C and illumination of 2000...3000 lx/cm2, at the same time the medium additionally contains the compound bis[N '-(2-hydroxy-kO-3-carboxybenzylidene)pyridine-3-carbohydrazidate(-1)-k2N',O]iron(III) perchlorate - water (4/5) in a concentration of 0.009...0.01 g/ IT.
Rezultatul tehnic al invenţiei constă în majorarea conţinutului de fenoli în biomasa microalgei Porphyridium cruentum, care este de 14,2…14,3 echivalent mg acid galic /g biomasă, conform metodei Folin-Ciocalteu, ceea ce reprezintă un avantaj de 20…21% în comparaţie cu cea mai apropiată soluţie. Administrarea compusului din prima zi de cultivare stimulează acumularea componentelor fenolice în biomasa microalgei, care sporesc valoarea biomasei de Porphyridium în calitate de producător de antioxidanţi. The technical result of the invention consists in increasing the content of phenols in the biomass of the microalga Porphyridium cruentum, which is 14.2...14.3 mg gallic acid equivalent /g biomass, according to the Folin-Ciocalteu method, which represents an advantage of 20...21% compared to the closest solution. Administration of the compound from the first day of cultivation stimulates the accumulation of phenolic components in the microalgae biomass, which increase the value of the Porphyridium biomass as an antioxidant producer.
Rezultatul invenţiei este condiţionat de aplicarea, pentru prima dată, a compusului bis[N′-(2-hidroxi-κO-3-carboxibenziliden)piridin-3-carbohidrazidat(-1)-κ2N′,O]fier(III) perclorat - apă (4/5) în calitate de stimulator în formarea componentelor fenolice în biomasa microalgei Porphyridium cruentum. The result of the invention is conditioned by the application, for the first time, of the compound bis[N′-(2-hydroxy-κO-3-carboxybenzylidene)pyridine-3-carbohydrazidate(-1)-κ2N′,O]iron(III) perchlorate - water (4/5) as a stimulator in the formation of phenolic components in the biomass of the microalgae Porphyridium cruentum.
Compusul revendicat se obţine la interacţiunea soluţiei metanolice de Fe(ClO4)3·xH2O cu soluţia de nicotinoilhidrazona-2-hidroxi-3-carboxibenzaldehidă (H2L) în amestec de solvenţi metanol-dimetilformamidă (10:1 după volum) în raport molar de 1:2. Reacţia decurge timp de 10 min. The claimed compound is obtained by the interaction of the methanolic solution of Fe(ClO4)3xH2O with the solution of nicotinoylhydrazone-2-hydroxy-3-carboxybenzaldehyde (H2L) in a mixture of methanol-dimethylformamide solvents (10:1 by volume) in a molar ratio of 1 :2. The reaction proceeds for 10 min.
Procedeul de obţinere a compusului revendicat este simplu în executare, substanţele iniţiale sunt accesibile, randamentul constituie 60%. Compusul este stabil la contact cu aerul, solubil în dimetilsulfoxidă, puţin solubil în dimetilformamidă, practic insolubil în alcooli şi apă. The procedure for obtaining the claimed compound is simple in execution, the initial substances are accessible, the yield is 60%. The compound is stable in contact with air, soluble in dimethylsulfoxide, slightly soluble in dimethylformamide, practically insoluble in alcohols and water.
Exemplu de obţinere a compusului bis[N′-(2-hidroxi-κO-3-carboxibenziliden)piridin-3-carbohidrazidat(-1)-κ2N′,O]fier(III) perclorat - apă (4/5) Example of obtaining the compound bis[N′-(2-hydroxy-κO-3-carboxybenzylidene)pyridine-3-carbohydrazidate(-1)-κ2N′,O]iron(III) perchlorate - water (4/5)
La soluţia de 0,23 g (0,5 mmol) de Fe(ClO4)3·xH2O în 5 ml de metanol se adaugă 0,28 g (1,0 mmol) de nicotinoilhidrazona-2-hidroxi-3-carboxibenzaldehidă (H2L). Amestecul obţinut se încălzeşte pe o baie marină cu agitare permanentă timp de 10 min. La răcire din soluţia de culoare cafenie-închisă se obţine un precipitat fin de culoare neagră, care a fost separat, spălat cu metanol, eter dietilic şi uscat în aer. Randamentul 0,23 g (60%). 0.28 g (1.0 mmol) of nicotinoylhydrazone-2-hydroxy-3-carboxybenzaldehyde (H2L ). The obtained mixture is heated on a sea bath with constant stirring for 10 min. On cooling, a fine black precipitate is obtained from the dark brown solution, which was separated, washed with methanol, diethyl ether and dried in air. Yield 0.23 g (60%).
S-a determinat, %: Fe 7,35, C 45,42 H 3,26; N 11,02. Pentru C28H22,5FeN6O13,5Cl s-a calculat, %: Fe 7,48, C 45,06; H 3,04; N 11,26. It was determined, %: Fe 7.35, C 45.42 H 3.26; N 11.02. For C28H22.5FeN6O13.5Cl it was calculated, %: Fe 7.48, C 45.06; H 3.04; No. 11,26.
Cercetarea sub microscop a compusului coordinativ sintetizat demonstrează că acesta posedă omogenitate fazică. Pentru a elucida compoziţia şi structura lui au fost aplicate metoda analizei elementelor, spectroscopia în IR şi analiza cu raze X. Microscopic examination of the synthesized coordinating compound demonstrates that it possesses phase homogeneity. In order to elucidate its composition and structure, the elemental analysis method, IR spectroscopy and X-ray analysis were applied.
În spectrul IR al ligandului necoordinat se manifestă benzi de absorbţie la 3384, 3210 şi 2416…1929cm-1, care corespund oscilaţiilor de valenţă ale grupelor ν(OH), ν(NH) şi PyH+, respectiv. În regiunea 1800…1660 cm-1, unde de regulă se manifestă benzile de absorbţie ν(C=O) a grupei carboxilice şi celei amidice libere lipsesc benzile respective. Acestea sunt deplasate la 1657 şi 1644 cm-1, fapt ce denotă asocierea acestor grupe. În spectrul compusului fierului dispare banda de absorbţie ν(NH) şi apare ν(C=O) cea a grupei carboxilice la 1710 cm-1, iar banda ν(C=O) de origine amidică dispare. Se menţine absorbţia la 2651 şi 2163 cm-1 caracteristică pentru oscilaţiile PyH+. Datele spectroscopiei IR dovedesc faptul că la coordinarea ligandului în fragmentul amidic are loc procesul de enolizare cu transferul protonului de la grupa NH la azotul heterociclic, formând PyH+ (S. B. Kalia, K. Lumba, G. Kaushal, M. Sharma. Indian J. Chem., 2007, 46A, p. 1233-1239; A. Sy, M. Dieng, I. E. Thiam, M. Gayea, and P. Retailleau. Acta Cryst., 2013, E69, m108; Беллами Л. Инфракрасные спектры сложных молекул. Изд-во Иностранной литературы. Москва, 1963, 590 p.). Banda foarte intensă de la 1080 cm-1 dovedeşte prezenţa ionului ClO4 - în sfera externă de coordinare. The IR spectrum of the non-coordinated ligand shows absorption bands at 3384, 3210 and 2416...1929cm-1, which correspond to the valence oscillations of the ν(OH), ν(NH) and PyH+ groups, respectively. In the region 1800...1660 cm-1, where the ν(C=O) absorption bands of the carboxylic group and the free amide group usually appear, the respective bands are missing. They are shifted to 1657 and 1644 cm-1, which indicates the association of these groups. In the spectrum of the iron compound, the ν(NH) absorption band disappears and ν(C=O) that of the carboxylic group appears at 1710 cm-1, and the ν(C=O) band of amide origin disappears. The absorption at 2651 and 2163 cm-1 characteristic for PyH+ oscillations is maintained. IR spectroscopy data prove that upon coordination of the ligand in the amide fragment, the enolization process occurs with the transfer of the proton from the NH group to the heterocyclic nitrogen, forming PyH+ (S. B. Kalia, K. Lumba, G. Kaushal, M. Sharma. Indian J. Chem ., 2007, 46A, p. 1233-1239; A. Sy, M. Dieng, I. E. Thiam, M. Gayea, and P. Retailleau. Acta Cryst., 2013, E69, m108; Bellamy L. Infrared spectra of complex molecules. Изд-во Иностранной литературы. Moscow, 1963, 590 p.). The very intense band at 1080 cm-1 proves the presence of the ClO4- ion in the external coordination sphere.
Astfel, în baza rezultatelor analizei elementelor şi cercetărilor spectroscopice a fost stabilită compoziţia şi structura compusului revendicat. Ionul de fier(III) manifestă numărul de coordinare 6 cu două molecule de ligand tridentat ONO şi are o configuraţie spaţială octaedrică cu ionul perclorat în sfera externă. Thus, based on the results of elemental analysis and spectroscopic research, the composition and structure of the claimed compound was established. The iron(III) ion exhibits coordination number 6 with two ONO tridentate ligand molecules and has an octahedral spatial configuration with the perchlorate ion in the outer sphere.
Aceste concluzii sunt confirmate şi de studiul cu raze X în baza unui monocristal, a cărui structură moleculară este prezentată în figură. Pentru analiza cu raze X a fost selectat un cristal de culoare cafenie-închisă, sub formă de prisme pătrate. Parametrii celulei elementare şi materialul experimental a fost obţinut la aparatul „Bruker SMART APEX” la iradierea monocromatică µ0kα 0,71073. Cristalul face parte din grupa spaţială P-1, cu următorii parametri ai celulei elementare: a = 10,868(2)Å, b = 12,143(2)Å, c = 13,632(3)Å, αcalc,= 1,616 mg/mm3, Z = 2 cu compoziţia C28H22,5ClFeN6O13,25. Setul de intensităţi 9067, dintre care 4306 independente. Factorul de divergenţă final Rint este egal cu 1,091. These conclusions are also confirmed by the X-ray study based on a single crystal, whose molecular structure is shown in the figure. A dark brown crystal in the form of square prisms was selected for X-ray analysis. The parameters of the elementary cell and the experimental material were obtained with the "Bruker SMART APEX" device at monochromatic irradiation µ0kα 0.71073. The crystal is part of space group P-1, with the following unit cell parameters: a = 10.868(2)Å, b = 12.143(2)Å, c = 13.632(3)Å, αcalc,= 1.616 mg/mm3, Z = 2 with the composition C28H22.5ClFeN6O13.25. The set of 9067 intensities, of which 4306 are independent. The final divergence factor Rint is equal to 1.091.
Exemple de aplicare a compusului bis[N′-(2-hidroxi-κO-3-carboxibenziliden)piridin-3-carbohidrazidat(-1)-κ2N′,O]fier(III) perclorat - apă (4/5) în calitate de stimulator al acumulării componentelor fenolice în biomasa microalgei Porphyridium cruentum. Examples of application of the compound bis[N′-(2-hydroxy-κO-3-carboxybenzylidene)pyridine-3-carbohydrazide(-1)-κ2N′,O]iron(III) perchlorate - water (4/5) in quality stimulator of the accumulation of phenolic components in the biomass of the microalgae Porphyridium cruentum.
Exemplul 1 Example 1
Se prepară mediul mineral nutritiv cu următorul conţinut al componentelor (g/L): NaNO3 - 5,0; NaCl - 7,0; KCl - 7,5; MgSO4·7H2O - 1,8; Ca(NO3)2·4H2O - 0,15; KBr - 0,05; KI - 0,05; K2HPO4 - 0,2; ZnSO4·5H2O - 0,00002; CuSO4·5H2O - 0,00005; MnSO4·5H2O - 0,0003; H3BO3 - 0,0006; MoO3 - 0,00002; NaVO3 - 0,00005 şi apă distilată până la 1L. În prima zi de cultivare, la suspensia de Porphyridium, în calitate de stimulator al biosintezei fenolilor se adaugă bis[N′-(2-hidroxi-κO-3-carboxibenziliden)piridin-3-carbohidrazidat(-1)-κ2N′,O]fier(III) perclorat - apă (4/5) în concentraţia de 0,009 g/L. Cultivarea se efectuează în baloane Erlenmayer a câte 1000 mL cu 500 mL suspensie în următoarele condiţii: pH-ul 6,8…7,2, temperatura de 23…25°C, iluminarea de 2000…3000 lx/cm2, la agitare lentă periodică. La ziua a 10-a, biomasa de Porphyridium se separă de lichidul cultural, se supune demineralizării şi se standardizează după biomasă. În biomasa obţinută se determină conţinutul fenolilor cu aplicarea metodei Folin-Ciocalteu. Prepare the nutritious mineral medium with the following content of components (g/L): NaNO3 - 5.0; NaCl - 7.0; KCl - 7.5; MgSO4·7H2O - 1.8; Ca(NO3)2·4H2O - 0.15; KBr - 0.05; KI - 0.05; K2HPO4 - 0.2; ZnSO4·5H2O - 0.00002; CuSO4·5H2O - 0.00005; MnSO4·5H2O - 0.0003; H3BO3 - 0.0006; MoO3 - 0.00002; NaVO3 - 0.00005 and distilled water up to 1L. On the first day of cultivation, bis[N′-(2-hydroxy-κO-3-carboxybenzylidene)pyridine-3-carbohydrazide(-1)-κ2N′,O ]iron(III) perchlorate - water (4/5) in the concentration of 0.009 g/L. Cultivation is carried out in Erlenmayer flasks of 1000 mL with 500 mL of suspension under the following conditions: pH 6.8...7.2, temperature 23...25°C, illumination 2000...3000 lx/cm2, with periodic slow stirring . On day 10, the Porphyridium biomass is separated from the culture liquid, subjected to demineralization and standardized by biomass. In the biomass obtained, the content of phenols is determined by applying the Folin-Ciocalteu method.
Biomasa de Porphyridium conţine 14,2±0,02 (p<0,5) mg acid galic echivalent/g biomasă, faţă de 11,8 mg acid galic echivalent/g biomasă în cazul celei mai apropiate soluţii (vezi tabelul). Sporul conţinutului de fenoli este de 20%. Porphyridium biomass contains 14.2±0.02 (p<0.5) mg gallic acid equivalent/g biomass, compared to 11.8 mg gallic acid equivalent/g biomass in the case of the closest solution (see table). The increase in phenol content is 20%.
Exemplul 2 Example 2
Se prepară mediul mineral nutritiv cu următorul conţinut al componentelor (g/L): NaNO3 - 5,0; NaCl - 7,0; KCl - 7,5; MgSO4·7H2O - 1,8; Ca(NO3)2·4H2O - 0,15; KBr - 0,05; KI - 0,05; K2HPO4 - 0,2; ZnSO4·5H2O - 0,00002; CuSO4·5H2O - 0,00005; MnSO4·5H2O - 0,0003; H3BO3 - 0,0006; MoO3 - 0,00002; NaVO3 - 0,00005 şi apă distilată până la 1L. În prima zi de cultivare, la suspensia de Porphyridium, în calitate de stimulator al biosintezei fenolilor, se adaugă compusul bis[N′-(2-hidroxi-κO-3-carboxibenziliden)piridin-3-carbohidrazidat(-1)-κ2N′,O]fier(III) perclorat - apă (4/5) în concentraţie de 0,01 g/L. Cultivarea se efectuează în baloane Erlenmayer a câte 1000 mL cu 500 mL suspensie în următoarele condiţii: pH-ul 6,8…7,2, temperatura de 23…25°C, iluminarea de 2000…3000 lx/cm2, la agitare lentă periodică. La ziua a 10-a, biomasa de Porphyridium se separă de lichidul cultural, se supune demineralizării şi se standardizează după biomasă. În biomasa obţinută se determină conţinutul fenolilor cu aplicarea metodei Folin-Ciocalteu. Prepare the nutritious mineral medium with the following content of components (g/L): NaNO3 - 5.0; NaCl - 7.0; KCl - 7.5; MgSO4·7H2O - 1.8; Ca(NO3)2·4H2O - 0.15; KBr - 0.05; KI - 0.05; K2HPO4 - 0.2; ZnSO4·5H2O - 0.00002; CuSO4·5H2O - 0.00005; MnSO4·5H2O - 0.0003; H3BO3 - 0.0006; MoO3 - 0.00002; NaVO3 - 0.00005 and distilled water up to 1L. On the first day of cultivation, the compound bis[N′-(2-hydroxy-κO-3-carboxybenzylidene)pyridine-3-carbohydrazide(-1)-κ2N′ is added to the Porphyridium suspension, as a stimulator of the biosynthesis of phenols ,O]iron(III) perchlorate - water (4/5) in a concentration of 0.01 g/L. Cultivation is carried out in Erlenmayer flasks of 1000 mL with 500 mL of suspension under the following conditions: pH 6.8...7.2, temperature 23...25°C, illumination 2000...3000 lx/cm2, with periodic slow stirring . On day 10, the Porphyridium biomass is separated from the culture liquid, subjected to demineralization and standardized by biomass. In the biomass obtained, the content of phenols is determined by applying the Folin-Ciocalteu method.
Biomasa de Porphyridium conţine 14,3±0,08 (p<0,5) mg acid galic echivalent/g biomasă, faţă de 11,8 mg acid galic echivalent/g biomasă în cazul celei mai apropiate soluţii (vezi tabelul). Sporul conţinutului de fenoli este de 21%. Porphyridium biomass contains 14.3±0.08 (p<0.5) mg gallic acid equivalent/g biomass, compared to 11.8 mg gallic acid equivalent/g biomass in the case of the closest solution (see table). The increase in phenol content is 21%.
Tabel Table
Conţinutul de fenoli în biomasa de Porphyridium cruentum la cultivare conform procedeului revendicat şi celei mai apropiate soluţii The content of phenols in the biomass of Porphyridium cruentum when cultivated according to the claimed process and the closest solution
Procedeul utilizat Concentraţia compusului, g/L Conţinutul fenolilor, mg acid galic echivalent/g biomasă Conform celei mai apropiate soluţii 0,01 10,6…11,8 Conform soluţiei revendicate 0,009 14,2±0,02 0,01 14,3±0,08 The procedure used Compound concentration, g/L Phenol content, mg gallic acid equivalent/g biomass According to the closest solution 0.01 10.6...11.8 According to the claimed solution 0.009 14.2±0.02 0.01 14.3 ±0.08
Datele din tabel demonstrează o creştere a conţinutului de fenoli în biomasa de Porphyridium cruentum cu 20…21% conform procedeului revendicat, faţă de procedeul cel mai apropiat. Biomasa microalgei Porphyridium cruentum reprezintă o sursă modernă de substanţe antioxidante, iată de ce sporul conţinutului de fenoli este un factor al creşterii valorii microalgei Porphyridium în calitate de producător de substanţe cu efect antioxidant. The data in the table demonstrate an increase in the content of phenols in the biomass of Porphyridium cruentum by 20...21% according to the claimed process, compared to the closest process. The biomass of the microalga Porphyridium cruentum represents a modern source of antioxidant substances, this is why the increase in the content of phenols is a factor in the increase in the value of the microalga Porphyridium as a producer of substances with an antioxidant effect.
Proprietăţile compusului bis[N′-(2-hidroxi-κO-3-carboxibenziliden)piridin-3-carbohidrazidat(-1)-κ2N′,O]fier(III) perclorat - apă (4/5) prezintă interes pentru biotehnologie în calitate de stimulator al producerii fenolilor, compuşi cu proprietăţi antioxidante, în biomasa microalgei Porphyridium cruentum. The properties of the compound bis[N′-(2-hydroxy-κO-3-carboxybenzylidene)pyridine-3-carbohydrazide(-1)-κ2N′,O]iron(III) perchlorate - water (4/5) are of interest for biotechnology in as a stimulator of the production of phenols, compounds with antioxidant properties, in the biomass of the microalgae Porphyridium cruentum.
1. Sadovnic D. Tehnologii de obţnere a preparatelor antioxidante şi antiradicalice din biomasa algei roşii Porphyridium cruentum CNM-AR-01. Teză de doctor în biologie, Chişinău, 2014 1. Sadovnic D. Technologies for obtaining antioxidant and antiradical preparations from the biomass of the red alga Porphyridium cruentum CNM-AR-01. PhD thesis in biology, Chisinau, 2014
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| MDA20140067A MD4367C1 (en) | 2014-07-09 | 2014-07-09 | Bis[N'-(2-hydroxy-kO-3-carboxybenzilidene)pyridine-3-carbohydrazidate(-1)-k2N',O]iron(III) perchlorate - water (4/5) and process for cultivation of microalga Porphyridium cruentum with its use |
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Citations (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4417415A (en) * | 1982-04-26 | 1983-11-29 | Battelle Development Corporation | Process for culturing a microalga, and extracting a polysaccharide therefrom |
| US5338673A (en) * | 1992-01-28 | 1994-08-16 | Commissariat A L'energie Atomique | Process for the selective production of polyunsaturated fatty acids from a culture of microalgae of the porphyridium cruentum |
| MD275G2 (en) * | 1993-12-30 | 1996-01-31 | Государственный Университет Молд0 | The stem of marine red algae Porphyridium cruentum CNM-AR 01-sourse of bioactive substances. |
| MD692F1 (en) * | 1995-07-10 | 1997-03-31 | Институт Микробиологии И Биотехнологии Академии Наук Молдовы | Medium for red algae Porphyridium cruentum cultivation |
| MD691G2 (en) * | 1995-07-05 | 1998-02-28 | Государственный Университет Молд0 | Medium for red algae Porphyridium cruentum cultivation |
| MD736G2 (en) * | 1997-01-04 | 1998-02-28 | Институт Микробиологии И Биотехнологии Академии Наук Молдовы | Process for obtaining of Porphyridium cruentum red alga biomass |
| MD909G2 (en) * | 1996-12-26 | 1998-09-30 | Государственный Университет Молд0 | Process for obtaining the red alga Porphyridium cruentum biomass |
| MD2386B2 (en) * | 2001-05-15 | 2004-02-29 | Inst De Microbiologie Al Acade | Process for cultivation of cyanobacterium Spirulina platensis |
| MD2409B2 (en) * | 2001-08-10 | 2004-03-31 | Univ De Stat Din Moldova | Process for cultivation of cyanobacterium Spirulina platensis |
| MD2861F1 (en) * | 2004-09-20 | 2005-09-30 | Universitatea De Stat Din Moldova | (µ-Imidazol-N, N?) - bis {4-bromo-2- [2- (oxyethylimino) -methyl] -phenolate (2 -) - copper}, which exhibits antifungal activity |
| UA37571U (en) * | 2006-05-03 | 2008-12-10 | Институт Биологии Южных Морей Им. О.О. Ковалевского Нан Украины | Method for cultivation of spirulina spirulina platensis (nordst.) geitl |
| US20100178674A1 (en) * | 2006-09-06 | 2010-07-15 | Institutio Tenologico y de estudios Superiores de Monterrey | Recovery and purification of b-phycoerythrin produced by porphyridium cruentum using two-aqueous-phase systems and isoelectric precipitation |
| MD4026C2 (en) * | 2009-11-13 | 2010-10-31 | Государственный Университет Молд0 | Hydrate-2-{[2-(2-hydroxyethylamino)-ethylimino]-methyl}-benzene-1,4-dihydroxy-(2-)copper and process for the cultivation of cyanobacterium Spirulina platensis |
| MD4043C2 (en) * | 2010-01-18 | 2010-12-31 | Государственный Университет Молд0 | Acetato-N-[2-(2-hydroxyethylamino)-ethyl]-salicylideneimino(1-)copper and process for cultivation of cyanobacterium Spirulina platensis |
| MD4069C1 (en) * | 2010-02-08 | 2011-04-30 | Государственный Университет Молд0 | Bromo-{3-[(2-hydroxy-5-nitro-benzylidene)-amino]-propane-1,2-dihydroxy}(1-)copper and process for the cultivation of cyanobacterium Spirulina platensis |
| MD4112B1 (en) * | 2010-05-17 | 2011-05-31 | Univ De Stat Din Moldova | Coordinative compounds of copper with 4-(dimethylphenyl)-thiosemicarbazones of 2-formylpyridine |
| MD4108C1 (en) * | 2010-09-27 | 2011-11-30 | Государственный Университет Молд0 | Process for cultivation of cyanobacterium Spirulina platensis |
| MD4127C1 (en) * | 2010-10-06 | 2012-04-30 | Государственный Университет Молд0 | Use of di(m-S)-bis{chloro-[1-(pyridine-2-yl)ethanone-4-methylthiosemicarbazonato(1-)]copper} as substance with antimicrobial activity against Staphylococcus aureus |
| MD4133C1 (en) * | 2010-07-19 | 2012-05-31 | Государственный Университет Молд0 | [(2-Carbamotioylhydrazone)propionato(2-)]-(4-aminobenzenesulfonamide)copper, manifesting antimicrobial activity against bacteria of the genus Bacillus cereus |
| MD4194B1 (en) * | 2012-01-23 | 2013-01-31 | Univ De Stat Din Moldova | Trinuclear coordinative compound tris{µ-¢3,5-dibromo-2-hydroxybenzylidene-4′-(pyridin-2-yl)-thiosemicarbazido(2-)]copper}hydrate, exhibiting antifungal activity against Candida albicans |
| MD4255B1 (en) * | 2012-11-05 | 2013-10-31 | Inst De Microbiologie Si Biotehnologie Al Academiei De Stiinte A Moldovei | Tetraisothiocyanatocobaltate of bis(nicotinoylhydrazone)-2,6-diformil-4-methylphenol(methanol)(aqua)cobalt(II) and process for cultivation of microalga Porphyridium cruentum with the use thereof |
| MD4253B1 (en) * | 2012-11-05 | 2013-10-31 | Inst De Microbiologie Si Biotehnologie Al Academiei De Stiinte A Moldovei | Sulphato-bis(nicotinoylhydrazone)-2,6-diacetylpyridin-cobalt(II )monomethanol trihydrate and process for cultivation of microalga Porphyridium cruentum with the use thereof |
| MD4254B1 (en) * | 2012-11-05 | 2013-10-31 | Inst De Microbiologie Si Biotehnologie Al Academiei De Stiinte A Moldovei | Hexafluorotitanate-bis[(dimethylglyoximato)-di-(thiocarbamide)cobalt(III)] dihydrate and process for cultivation of microalga Porphyridium cruentum with the use thereof |
| MD4278B1 (en) * | 2013-04-30 | 2014-03-31 | Institutul De Chimie Al Academiei De Ştiinţe A Moldovei | Compound bis{bis(dimethylglyoximato)chloro}-µ-3-formylpiridineisonicotinoylhydrazone-di-cobalt(III) and process for cultivation of microalga Porphyridium cruentum with its use |
| CN203668407U (en) * | 2013-11-04 | 2014-06-25 | 许昌县四杰生物科技有限公司 | Induced cultivation system for microalgae |
| CN104046567A (en) * | 2013-03-17 | 2014-09-17 | 中国石油化工股份有限公司 | Microalgae cultivation method and grease production method |
-
2014
- 2014-07-09 MD MDA20140067A patent/MD4367C1/en not_active IP Right Cessation
Patent Citations (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4417415A (en) * | 1982-04-26 | 1983-11-29 | Battelle Development Corporation | Process for culturing a microalga, and extracting a polysaccharide therefrom |
| US5338673A (en) * | 1992-01-28 | 1994-08-16 | Commissariat A L'energie Atomique | Process for the selective production of polyunsaturated fatty acids from a culture of microalgae of the porphyridium cruentum |
| MD275G2 (en) * | 1993-12-30 | 1996-01-31 | Государственный Университет Молд0 | The stem of marine red algae Porphyridium cruentum CNM-AR 01-sourse of bioactive substances. |
| MD691G2 (en) * | 1995-07-05 | 1998-02-28 | Государственный Университет Молд0 | Medium for red algae Porphyridium cruentum cultivation |
| MD692F1 (en) * | 1995-07-10 | 1997-03-31 | Институт Микробиологии И Биотехнологии Академии Наук Молдовы | Medium for red algae Porphyridium cruentum cultivation |
| MD909G2 (en) * | 1996-12-26 | 1998-09-30 | Государственный Университет Молд0 | Process for obtaining the red alga Porphyridium cruentum biomass |
| MD736G2 (en) * | 1997-01-04 | 1998-02-28 | Институт Микробиологии И Биотехнологии Академии Наук Молдовы | Process for obtaining of Porphyridium cruentum red alga biomass |
| MD2386B2 (en) * | 2001-05-15 | 2004-02-29 | Inst De Microbiologie Al Acade | Process for cultivation of cyanobacterium Spirulina platensis |
| MD2409B2 (en) * | 2001-08-10 | 2004-03-31 | Univ De Stat Din Moldova | Process for cultivation of cyanobacterium Spirulina platensis |
| MD2861F1 (en) * | 2004-09-20 | 2005-09-30 | Universitatea De Stat Din Moldova | (µ-Imidazol-N, N?) - bis {4-bromo-2- [2- (oxyethylimino) -methyl] -phenolate (2 -) - copper}, which exhibits antifungal activity |
| UA37571U (en) * | 2006-05-03 | 2008-12-10 | Институт Биологии Южных Морей Им. О.О. Ковалевского Нан Украины | Method for cultivation of spirulina spirulina platensis (nordst.) geitl |
| US20100178674A1 (en) * | 2006-09-06 | 2010-07-15 | Institutio Tenologico y de estudios Superiores de Monterrey | Recovery and purification of b-phycoerythrin produced by porphyridium cruentum using two-aqueous-phase systems and isoelectric precipitation |
| MD4026C2 (en) * | 2009-11-13 | 2010-10-31 | Государственный Университет Молд0 | Hydrate-2-{[2-(2-hydroxyethylamino)-ethylimino]-methyl}-benzene-1,4-dihydroxy-(2-)copper and process for the cultivation of cyanobacterium Spirulina platensis |
| MD4043C2 (en) * | 2010-01-18 | 2010-12-31 | Государственный Университет Молд0 | Acetato-N-[2-(2-hydroxyethylamino)-ethyl]-salicylideneimino(1-)copper and process for cultivation of cyanobacterium Spirulina platensis |
| MD4069C1 (en) * | 2010-02-08 | 2011-04-30 | Государственный Университет Молд0 | Bromo-{3-[(2-hydroxy-5-nitro-benzylidene)-amino]-propane-1,2-dihydroxy}(1-)copper and process for the cultivation of cyanobacterium Spirulina platensis |
| MD4112B1 (en) * | 2010-05-17 | 2011-05-31 | Univ De Stat Din Moldova | Coordinative compounds of copper with 4-(dimethylphenyl)-thiosemicarbazones of 2-formylpyridine |
| MD4133C1 (en) * | 2010-07-19 | 2012-05-31 | Государственный Университет Молд0 | [(2-Carbamotioylhydrazone)propionato(2-)]-(4-aminobenzenesulfonamide)copper, manifesting antimicrobial activity against bacteria of the genus Bacillus cereus |
| MD4108C1 (en) * | 2010-09-27 | 2011-11-30 | Государственный Университет Молд0 | Process for cultivation of cyanobacterium Spirulina platensis |
| MD4127C1 (en) * | 2010-10-06 | 2012-04-30 | Государственный Университет Молд0 | Use of di(m-S)-bis{chloro-[1-(pyridine-2-yl)ethanone-4-methylthiosemicarbazonato(1-)]copper} as substance with antimicrobial activity against Staphylococcus aureus |
| MD4194B1 (en) * | 2012-01-23 | 2013-01-31 | Univ De Stat Din Moldova | Trinuclear coordinative compound tris{µ-¢3,5-dibromo-2-hydroxybenzylidene-4′-(pyridin-2-yl)-thiosemicarbazido(2-)]copper}hydrate, exhibiting antifungal activity against Candida albicans |
| MD4255B1 (en) * | 2012-11-05 | 2013-10-31 | Inst De Microbiologie Si Biotehnologie Al Academiei De Stiinte A Moldovei | Tetraisothiocyanatocobaltate of bis(nicotinoylhydrazone)-2,6-diformil-4-methylphenol(methanol)(aqua)cobalt(II) and process for cultivation of microalga Porphyridium cruentum with the use thereof |
| MD4253B1 (en) * | 2012-11-05 | 2013-10-31 | Inst De Microbiologie Si Biotehnologie Al Academiei De Stiinte A Moldovei | Sulphato-bis(nicotinoylhydrazone)-2,6-diacetylpyridin-cobalt(II )monomethanol trihydrate and process for cultivation of microalga Porphyridium cruentum with the use thereof |
| MD4254B1 (en) * | 2012-11-05 | 2013-10-31 | Inst De Microbiologie Si Biotehnologie Al Academiei De Stiinte A Moldovei | Hexafluorotitanate-bis[(dimethylglyoximato)-di-(thiocarbamide)cobalt(III)] dihydrate and process for cultivation of microalga Porphyridium cruentum with the use thereof |
| CN104046567A (en) * | 2013-03-17 | 2014-09-17 | 中国石油化工股份有限公司 | Microalgae cultivation method and grease production method |
| MD4278B1 (en) * | 2013-04-30 | 2014-03-31 | Institutul De Chimie Al Academiei De Ştiinţe A Moldovei | Compound bis{bis(dimethylglyoximato)chloro}-µ-3-formylpiridineisonicotinoylhydrazone-di-cobalt(III) and process for cultivation of microalga Porphyridium cruentum with its use |
| CN203668407U (en) * | 2013-11-04 | 2014-06-25 | 许昌县四杰生物科技有限公司 | Induced cultivation system for microalgae |
Non-Patent Citations (1)
| Title |
|---|
| Sadovnic D. Tehnologii de obţnere a preparatelor antioxidante şi antiradicalice din biomasa algei roşii Porphyridium cruentum CNM-AR-01. Teză de doctor în biologie, Chişinău, 2014 * |
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