CN203668407U - Induced cultivation system for microalgae - Google Patents
Induced cultivation system for microalgae Download PDFInfo
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- CN203668407U CN203668407U CN201320687934.8U CN201320687934U CN203668407U CN 203668407 U CN203668407 U CN 203668407U CN 201320687934 U CN201320687934 U CN 201320687934U CN 203668407 U CN203668407 U CN 203668407U
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Abstract
The utility model discloses an induced cultivation system for microalgae. In a tubular reactor, an outlet of a first tubular structure is communicated with the upper part of a buffer tank through a drain pipe, an inlet of a last tubular structure is communicated with the bottom of the buffer tank through a feed pipe, a water pump is mounted on the feed pipe, meanwhile, the feed pipe is communicated with an algae liquid sample measuring tank, sensors are connected with a control decision system respectively, and a control terminal of the control decision system is connected with a control of the water pump. The induced cultivation system for microalgae can finish objective product induction in conventional circular flow type microalgae cultivation facilities, cultivation equipment is not required to be replaced or added for objective product induction, investment and occupied land are saved, operation steps and energy consumption are reduced, and automatic, continuous, fast and efficient production of economic microalgae can be achieved.
Description
Technical field
The utility model relates to both culturing microalgae technical field, is specifically related to a kind of micro-algae induction cultivating system.
Background technology
Phycophyta not only can catch airborne carbonic acid gas, contributes to control greenhouse gas emission, and according to statistics, the foster algae field of taking up an area 1 square kilometre can year 50000 tons of carbonic acid gas of processing.The exploitation of micro-algae not only can reduce discharging CO
2, and can utilize the characteristic of micro-algae accumulation carbohydrate, pigment and grease, carry out the exploitation of micro-algae biological fodder, food, medicine, bio-ethanol and biofuel, thereby improve and enrich the demand of the mankind to food, medicine, the energy.The exploitation of the micro-algae of high value and the micro-algae of bioenergy is the current focus of research, such as the chlorella of salt algae, Haematocoocus Pluvialls, Porphyridium cruentum and the micro-algae of the energy of the micro-algae of high value, grid algae, diatom etc. are all the emphasis of research at present.
The cultivation of micro-algae or cultivation be exactly micro-algae by photosynthesis CO
2change organism into water, thereby carry out the process of Growth and reproduction.In the normal culturing process of micro-algae, first micro-algae accumulates carbohydrate and protein, take schizogamy as main; In the time of the cultivation micro-algae of high value and the micro-algae of the energy, while needing special condition to make micro-algae accumulation object product, need to change culture condition, make it to be unfavorable for micro algae growth or existence, now micro-algae starts to accumulate starch, pigment and grease, and these conditions comprise shortage (N or P) or low temperature, the high light etc. of special nutrition material; So the cultivation of the micro-algae of the energy need to be carried out the induction of grease in breeding process, certain or some growth conditionss that also change in microalga cultivation process carry out restricted cultivation, thus the accumulation of induction starch, pigment or grease.Therefore can find out this slightly the cultivation of algae not only need the Fast-propagation of micro-algae, also need micro-algae to accumulate fast starch, pigment or grease, thereby produce efficiently required biomaterial.
In current micro-algae culturing process, the micro-algae induction mode the most generally adopting has three kinds.The first, artificially reduce N content in the required nutritive medium of micro algae growth, cause micro-algae can not synthetic protein, thereby micro-algae can not be divided and breed, its photosynthetic energy and carbon source change into starch or oil and fat accumulation; Though the method can make micro-algae accumulation starch or grease, but integration time is oversize, causes production cost excessive.The second, can adopt high-intensity sunlight or artificial light to carry out photoinduction, under high light effect, the luminous energy that micro-phycochrome absorbs is much larger than photosynthetic reaction centre institute energy requirement, thereby causes micro-algae body to receive strong oxidation, and then causes damage; Not impaired for protection body, micro-algae starts to change metabolic way, and outstanding feature is, micro-algae starts to reduce chlorophyllous content, synthetic other coloured pigments, and start to accumulate starch, Synthetic Oil is stored unnecessary heat then; Though the method can make micro-algae accumulation starch, pigment or grease, too fast but high light also may make algae liquid temp raise, and causes microalgae cell death, high light is induced in the time of nutritional sufficiency in addition, and effect is also bad.The 3rd, first and second kinds of methods are combined to use, this method is the most general in the micro-algae of photosynthetic organism reactor culturing, the method is exactly to carry out two-step approach cultivation by the reactor of two kinds of light paths, the first step is to provide sufficient nutrient and light to micro-algae in wider light path reactor, make micro-algae Fast-propagation, when N content is lower in algae liquid, carry out second step cultivation; Second step is exactly that the algae liquid in reactor in the first step is transferred in another kind of narrow reactor and cultivated, and now, the N content in algae liquid is not only low, and narrow light path reactor also provides more sufficient luminous energy to cell, makes its Rapid Accumulation starch or grease; Adopt in this way, object product accumulation efficiency is higher, but fixed investment cost (reactor of narrow light path) is higher, simultaneously, increased floor space, induction object product efficiency of conversion also depends on the applicable ratio to narrow light path by wide light path, as, if induction light path is wide, induction efficiency can be not obvious; Induction light path is narrow, can cause light to suppress to the algae of reactor surface, affects output.
Utility model content
The purpose of this utility model is to provide a kind of cultivating system that can complete quick object product induction in normal flow dynamic formula circulation both culturing microalgae facility, make the micro-algae of the energy can in circulating of routine cultivated facility, complete the accumulation of object product, without special induction cultivation equipment, the facility of extra construction, effectively reduce this aquaculture cost and equipment occupation of land of algae slightly, significantly improve the economy of both culturing microalgae.
In order to achieve the above object, the technical scheme that the utility model adopts is: a kind of micro-algae induction cultivating system, the head and the tail of the tubular structure of arranging are side by side communicated with to formation tubular reactor successively, the outlet of first tubular structure is communicated with surge tank top by drain pipe, the entrance of an end tubular structure is communicated with the bottom of surge tank by liquid-inlet pipe, water pump is installed on liquid-inlet pipe, simultaneously, front and back position at liquid-inlet pipe is connected with respectively test sample pipe, test sample Guan Yuzao liquid test sample pond is communicated with, default valve is installed on test sample pipe, and be provided with the sensor that algae liquid test sample pond is carried out to signals collecting, each sensor is connected with the signal input part of control decision system respectively, the control end of control decision system is connected with the control end of water pump.
Described sensor comprises temperature sensor, pH sensor and optical density (OD) instrument.
In described clear tubular structure, be provided with intensity of illumination probe, intensity of illumination probe is connected with control decision system by signal wire.
The utility model has the advantages that:
1, the utility model proposes the system of single stage method cultivation special economic algae (salt algae, Haematocoocus Pluvialls or oily algae etc.), can in regular circulation flowing-type both culturing microalgae facility, complete the induction of object product, without changing or increase cultivation equipment for induction object product, reduce investment outlay and take up an area, reduce operation steps and energy consumption, can realize economic micro-algae and produce automatically, continuously, fast and efficiently.
2, can make micro-algae efficient accumulation object product (polysaccharide, pigment or grease etc.) in fast breeding process.Can the unit's of making object product volume cost significantly reduce.
3, complete by a set of self-feedback regulation and control system, can automatically adjust all parameters of breeding technique according to environment and micro algae growth situation.
accompanying drawing explanation:
Fig. 1 is the overall structure of the micro-algae induction of the utility model cultivating system.
Number in the figure: 1 is clear tubular structure, 2 is surge tank, and 3 is water pump, and 4 is control decision system, and 5 is algae liquid test sample pond, and 6 is default valve, and 7 is temperature sensor, and 8 is pH sensor, and 9 is optical density (OD) instrument, and 10 is intensity of illumination probe, and 11 is feedback line.
Embodiment
In Fig. 1, micro-algae induction cultivating system is mainly by clear tubular structure 1, surge tank 2 and water pump 3 three part-structures, in figure, clear tubular structure 1 can be both culturing microalgae an airtight place is provided, utilize water pump 3 to circulate in tubular structure 1 as powered algae liquid, algae liquid is finally back in surge tank 2, through this step, micro-algae can absorb sun power and carry out photosynthesis in clear tubular structure 1, carries out Growth and reproduction.
The head and the tail of the tubular structure of arranging are side by side communicated with to formation tubular reactor successively, and in above-mentioned tubular reactor, the process that completes quick object product induction cultivation by micro-algae induction cultivating system specifically describes as follows:
System is set up an algae liquid test sample pond 5, and algae liquid test sample pond 5 is positioned in the bypass of a liquid line after water pump 3, and in the front and back end in algae liquid test sample pond 5, default valve 6 is held back.In algae liquid test sample pond 5, be provided with multiple electrode, need there are the electrodes such as temperature sensor 7, pH sensor 8, optical density (OD) 9 according to experiment, above-mentioned probe opposite side is connected with control decision system 4; And at bioreactor surface mounting intensity of illumination probe 10, be connected to equally control decision system 4.The algae liquid temp, the algae liquid ionic concn (N and P) that obtain by Real-Time Monitoring, algae liquid cell density and ambient lighting strength signal, enter control decision system 4, calculates when best algae flow velocity under precondition; This flow velocity signal to variable frequency pump 3, changes flow rate of liquid in tubular type bioreactor by feedback line 11, and adjustment microalgae cell growing environment is not subject to the impact of high light, high low temperature etc., keeps the best speed of growth.In the time that micro-algae enters grease induction breeding process, reduce with N, P ionic concn, control decision system 4 can be adjusted gradually and be reduced algae flow velocity by water pump 3, increase the residence time of microalgae cell in reactor, make frustule be subject to more strong illumination, induction microalgae cell accumulated polysaccharide, pigment or grease.So just guarantee that micro-algae can breed by Fast Growth in same system, also can reach by adjusting simple flow velocity the object of Rapid Accumulation object product.
For example, the present embodiment is tried out at somewhere, Henan open space, micro-algae kind of application is a kind of unicell green alga, the bore of tubular reactor is 5cm, the electrode adopting has temp probe, illumination probe, pH electrode, N ionic concn probe, optical density (OD) probe, the cultivation of employing sunlight, does not increase artificial lighting; The dress liquid cumulative volume of this system is 900L, and wherein the dress liquid of pipe amasss as 600L, and the dress liquid of surge tank amasss as 300L, and the total floor space of this system is 12m
2.
Be described with regard to this system single stage method cultivating microalgae below: first, need to substratum salt put into surge tank, then add tap water mixed culture medium, then algae kind good laboratory culture is put into surge tank, after together mixing, be transported to and in pipe, carry out Cyclic culture by water pump.The second, after normal cultivation, Controlling System is carried out the culture environment of regulator control system by the data of each electrode; If Temperature Setting is at 20～30 degree, decision system can be passed through temp probe feedback regulation flow velocity, makes temperature within the scope of this; System can be passed through pH electrode feedback regulation CO for another example
2intake and pass into the time; System can pop one's head in the residence time of adjustable micro-algal cell in pipe by illumination for another example, thereby avoids light to suppress, and increases photosynthetic efficiency.The 3rd, in the time that N ionic concn electrode shows that in algae liquid, N content is lower than a threshold values, system can slow down flow velocity automatically, increases the residence time of cell in pipe, realizes the synthetic of induction starch or grease; Certainly in the time that illumination probe display light photograph exceeds maximum setting range, system also can be for Cell protection, increases in time flow velocity and avoids it to be killed by excessive power.
Claims (3)
1. a micro-algae induction cultivating system, it is characterized in that: the head and the tail of the tubular structure of arranging are side by side communicated with to formation tubular reactor successively, the outlet of first tubular structure is communicated with surge tank top by drain pipe, the entrance of an end tubular structure is communicated with the bottom of surge tank by liquid-inlet pipe, water pump is installed on liquid-inlet pipe, simultaneously, front and back position at liquid-inlet pipe is connected with respectively test sample pipe, test sample Guan Yuzao liquid test sample pond is communicated with, default valve is installed on test sample pipe, and be provided with the sensor that algae liquid test sample pond is carried out to signals collecting, each sensor is connected with the signal input part of control decision system respectively, the control end of control decision system is connected with the control end of water pump.
2. micro-algae induction cultivating system according to claim 1, is characterized in that: described sensor comprises temperature sensor pH sensor and optical density (OD) instrument.
3. micro-algae induction cultivating system according to claim 1, is characterized in that: on described tubular structure, be provided with intensity of illumination probe, intensity of illumination probe is connected with control decision system by signal wire.
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| CN201320687934.8U CN203668407U (en) | 2013-11-04 | 2013-11-04 | Induced cultivation system for microalgae |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105087384A (en) * | 2014-05-15 | 2015-11-25 | 中国石油化工股份有限公司 | Method for culturing photosynthetic microbes and method for producing grease |
| MD4356C1 (en) * | 2014-07-09 | 2016-02-29 | Институт Химии Академии Наук Молдовы | Bis[N'-(2-hydroxy-kO-benzylidene)pyridine-4-carbohydrazidate(-1)-k2N',O]iron(III) nitrate - water (2/3) and process for cultivation of microalga Porphyridium cruentum with its use |
| MD4367C1 (en) * | 2014-07-09 | 2016-03-31 | Институт Химии Академии Наук Молдовы | Bis[N'-(2-hydroxy-kO-3-carboxybenzilidene)pyridine-3-carbohydrazidate(-1)-k2N',O]iron(III) perchlorate - water (4/5) and process for cultivation of microalga Porphyridium cruentum with its use |
| MD4366C1 (en) * | 2014-07-09 | 2016-03-31 | Институт Химии Академии Наук Молдовы | Bis[1-phenyl-3-methyl-6-(pyridinium-4-il)-4,5-diaza-hexa-1,3,5-triene-1-hydroxy-6-olato-O1,N4,O6]iron(II)sulphate tetrahydrate and process for cultivation of microalga Porphyridium cruentum with its use |
| MD4365C1 (en) * | 2014-07-09 | 2016-03-31 | Институт Химии Академии Наук Молдовы | Bis[1-phenyl-3-methyl-6-(pyridinium-4-yl)-4,5-diaza-hexa-1,3,5-triene-1,6-diolato-O1,N4,O6]iron(III) nitrate and process for cultivation of microalga Porphyridium cruentum with its use |
| CN106754316A (en) * | 2017-03-03 | 2017-05-31 | 江西中藻生物科技股份有限公司 | A kind of spirulina cultivation apparatus |
| CN106982676A (en) * | 2017-03-14 | 2017-07-28 | 新奥泛能网络科技股份有限公司 | A kind of energy supplying system |
-
2013
- 2013-11-04 CN CN201320687934.8U patent/CN203668407U/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105087384A (en) * | 2014-05-15 | 2015-11-25 | 中国石油化工股份有限公司 | Method for culturing photosynthetic microbes and method for producing grease |
| CN105087384B (en) * | 2014-05-15 | 2018-02-23 | 中国石油化工股份有限公司 | Cultivate the method for photosynthetic microorganism and the method for production grease |
| MD4356C1 (en) * | 2014-07-09 | 2016-02-29 | Институт Химии Академии Наук Молдовы | Bis[N'-(2-hydroxy-kO-benzylidene)pyridine-4-carbohydrazidate(-1)-k2N',O]iron(III) nitrate - water (2/3) and process for cultivation of microalga Porphyridium cruentum with its use |
| MD4367C1 (en) * | 2014-07-09 | 2016-03-31 | Институт Химии Академии Наук Молдовы | Bis[N'-(2-hydroxy-kO-3-carboxybenzilidene)pyridine-3-carbohydrazidate(-1)-k2N',O]iron(III) perchlorate - water (4/5) and process for cultivation of microalga Porphyridium cruentum with its use |
| MD4366C1 (en) * | 2014-07-09 | 2016-03-31 | Институт Химии Академии Наук Молдовы | Bis[1-phenyl-3-methyl-6-(pyridinium-4-il)-4,5-diaza-hexa-1,3,5-triene-1-hydroxy-6-olato-O1,N4,O6]iron(II)sulphate tetrahydrate and process for cultivation of microalga Porphyridium cruentum with its use |
| MD4365C1 (en) * | 2014-07-09 | 2016-03-31 | Институт Химии Академии Наук Молдовы | Bis[1-phenyl-3-methyl-6-(pyridinium-4-yl)-4,5-diaza-hexa-1,3,5-triene-1,6-diolato-O1,N4,O6]iron(III) nitrate and process for cultivation of microalga Porphyridium cruentum with its use |
| CN106754316A (en) * | 2017-03-03 | 2017-05-31 | 江西中藻生物科技股份有限公司 | A kind of spirulina cultivation apparatus |
| CN106982676A (en) * | 2017-03-14 | 2017-07-28 | 新奥泛能网络科技股份有限公司 | A kind of energy supplying system |
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