MD20140089A2 - Procedeu de analiză expres a activității dehidrogenazei în biomasa fermentată - Google Patents
Procedeu de analiză expres a activității dehidrogenazei în biomasa fermentatăInfo
- Publication number
- MD20140089A2 MD20140089A2 MDA20140089A MD20140089A MD20140089A2 MD 20140089 A2 MD20140089 A2 MD 20140089A2 MD A20140089 A MDA20140089 A MD A20140089A MD 20140089 A MD20140089 A MD 20140089A MD 20140089 A2 MD20140089 A2 MD 20140089A2
- Authority
- MD
- Moldova
- Prior art keywords
- ttc
- solution
- analyzed
- triphenyl
- mixture
- Prior art date
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Invenţia se referă la biotehnologie, şi anume la un procedeu expres de determinare a activităţii fermentului dehidrogenaza.Procedeul, conform invenţiei, prevede introducerea în proba analizată a soluţiei de glucoză şi 2,3,5-trifenil-tetrazoliu clorurat (TTC), incubarea amestecului în condiţii de vid, extragerea ulterioară cu alcool, determinarea colorimetrică a densităţii optice a soluţiei colorate de trifenil-formazan (TFF) formate în rezultatul reacţiei de dehidrogenare a TTC cu calcularea ulterioară a activităţii, totodată, în componenţa amestecului supus analizei se introduce scualenă, în cantitate de (5,0…5,5)10-4 % în raport cu volumul amestecului analizat, şi soluţie tampon de fosfat până la pH-ul 7,2, iar în calitate de substrat se utilizează soluţie de 0,2 M de glucoză şi 2% TTC, procesul de incubare având loc în condiţii mezofile la temperatura de 33±1°C timp de 25…30 min şi agitarea ulterioară prin scuturare timp de 5 min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDA20140089A MD4465C1 (ro) | 2014-08-22 | 2014-08-22 | Procedeu de determinare a activităţii dehidrogenazei în biomasă la fermentare |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MDA20140089A MD4465C1 (ro) | 2014-08-22 | 2014-08-22 | Procedeu de determinare a activităţii dehidrogenazei în biomasă la fermentare |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| MD20140089A2 true MD20140089A2 (ro) | 2016-02-29 |
| MD4465B1 MD4465B1 (ro) | 2017-01-31 |
| MD4465C1 MD4465C1 (ro) | 2017-08-31 |
Family
ID=55447188
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MDA20140089A MD4465C1 (ro) | 2014-08-22 | 2014-08-22 | Procedeu de determinare a activităţii dehidrogenazei în biomasă la fermentare |
Country Status (1)
| Country | Link |
|---|---|
| MD (1) | MD4465C1 (ro) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109580510A (zh) * | 2018-12-04 | 2019-04-05 | 天津大学 | 一种活性污泥ttc脱氢酶活性测定方法 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2053897A5 (en) * | 1969-07-09 | 1971-04-16 | American Hospital Supply Corp | Dehydrogenase activity determination with - dyeing composition |
| SU1043568A1 (ru) * | 1981-06-11 | 1983-09-23 | Московский Ордена Ленина,Ордена Октябрьской Революции И Ордена Трудового Красного Знамени Государственный Университет Им.М.В.Ломоносова | Способ определени активности дегидрогеназ крови |
| JPS60141299A (ja) * | 1983-12-28 | 1985-07-26 | Wako Pure Chem Ind Ltd | 脱水素酵素の活性度測定法 |
| RU2387996C1 (ru) * | 2008-09-22 | 2010-04-27 | Общество с ограниченной ответственностью "Научно-исследовательский институт природных газов и газовых технологий - Газпром ВНИИГАЗ" (ООО "ВНИИГАЗ") | Способ контроля очистки почв, загрязненных углеводородами, и нейтрализации углеводородных шламов посредством анализа активности дегидрогеназы |
| RU2476598C2 (ru) * | 2011-04-27 | 2013-02-27 | Федеральное государственное образовательное учреждение высшего профессионального образования "Северный (Арктический) федеральный университет" (С(А)ФУ) | Способ количественного определения дегидрогеназной активности микроорганизмов |
| CN103525896B (zh) * | 2013-09-27 | 2015-04-08 | 南京工业大学 | 一种基于ttc染色法的高活力酵母细胞定量筛选方法 |
-
2014
- 2014-08-22 MD MDA20140089A patent/MD4465C1/ro not_active IP Right Cessation
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109580510A (zh) * | 2018-12-04 | 2019-04-05 | 天津大学 | 一种活性污泥ttc脱氢酶活性测定方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| MD4465C1 (ro) | 2017-08-31 |
| MD4465B1 (ro) | 2017-01-31 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG4A | Patent for invention issued | ||
| KA4A | Patent for invention lapsed due to non-payment of fees (with right of restoration) | ||
| MM4A | Patent for invention definitely lapsed due to non-payment of fees |