LV10304B - Use of cross-linked crystals as a novel form of enzyme immobilization - Google Patents

Use of cross-linked crystals as a novel form of enzyme immobilization Download PDF

Info

Publication number
LV10304B
LV10304B LVP-93-807A LV930807A LV10304B LV 10304 B LV10304 B LV 10304B LV 930807 A LV930807 A LV 930807A LV 10304 B LV10304 B LV 10304B
Authority
LV
Latvia
Prior art keywords
enzyme
crystal
cross
immobilized
crystals
Prior art date
Application number
LVP-93-807A
Other languages
English (en)
Latvian (lv)
Other versions
LV10304A (lv
Inventor
Manuel A Navia
Clair Nancy L St
Original Assignee
Vertex Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vertex Pharma filed Critical Vertex Pharma
Publication of LV10304A publication Critical patent/LV10304A/xx
Publication of LV10304B publication Critical patent/LV10304B/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/06—Dipeptides
    • C07K5/06104—Dipeptides with the first amino acid being acidic
    • C07K5/06113—Asp- or Asn-amino acid
    • C07K5/06121—Asp- or Asn-amino acid the second amino acid being aromatic or cycloaliphatic
    • C07K5/0613—Aspartame
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2462—Lysozyme (3.2.1.17)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6448—Elastases, e.g. pancreatic elastase (3.4.21.36); leukocyte elastase (3.4.31.37)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C12N9/82—Asparaginase (3.5.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/002—Electrode membranes
    • C12Q1/003—Functionalisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Liquid Crystal Substances (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)
  • Piezo-Electric Or Mechanical Vibrators, Or Delay Or Filter Circuits (AREA)
LVP-93-807A 1990-08-03 1993-06-30 Use of cross-linked crystals as a novel form of enzyme immobilization LV10304B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US56228090A 1990-08-03 1990-08-03
PCT/US1991/005415 WO1992002617A1 (fr) 1990-08-03 1991-07-30 Emploi de cristaux reticules comme nouvelle forme d'immobilisation d'enzymes

Publications (2)

Publication Number Publication Date
LV10304A LV10304A (lv) 1994-10-20
LV10304B true LV10304B (en) 1995-06-20

Family

ID=24245600

Family Applications (1)

Application Number Title Priority Date Filing Date
LVP-93-807A LV10304B (en) 1990-08-03 1993-06-30 Use of cross-linked crystals as a novel form of enzyme immobilization

Country Status (26)

Country Link
EP (2) EP0861888B1 (fr)
JP (2) JPH05508549A (fr)
AT (2) ATE323158T1 (fr)
AU (1) AU662104B2 (fr)
BR (1) BR9106732A (fr)
CA (1) CA2087730C (fr)
CZ (1) CZ12393A3 (fr)
DE (2) DE69133521T2 (fr)
DK (2) DK0861888T3 (fr)
ES (2) ES2199933T3 (fr)
FI (1) FI120099B (fr)
HK (2) HK1012411A1 (fr)
HU (2) HUT65904A (fr)
IE (1) IE912767A1 (fr)
IL (1) IL99046A (fr)
LT (1) LT3366B (fr)
LV (1) LV10304B (fr)
MD (1) MD1592B2 (fr)
NO (1) NO930360L (fr)
PT (1) PT98564B (fr)
RU (1) RU2124052C1 (fr)
SG (1) SG70966A1 (fr)
SK (1) SK283185B6 (fr)
TJ (1) TJ210R3 (fr)
UA (1) UA27035C2 (fr)
WO (1) WO1992002617A1 (fr)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5932212A (en) * 1996-05-24 1999-08-03 Altus Biologics, Inc. Crosslinked protein crystal formulations and their use as catalysts in organic solvents
AU4738197A (en) * 1996-09-24 1998-04-17 Altus Biologics Inc. Crosslinked protein crystals as universal separation media
US6140475A (en) * 1997-04-11 2000-10-31 Altus Biologics Inc. Controlled dissolution crosslinked protein crystals
US6500933B1 (en) 1997-09-05 2002-12-31 Altus Biologics Inc. Methods of preparing carbohydrate crosslinked glycoprotein crystals
ES2306477T3 (es) * 1997-09-05 2008-11-01 Altus Pharmaceuticals Inc. Cristales de glicoproteina reticulados por carbohidratos.
US6979561B1 (en) 1998-10-09 2005-12-27 Gilead Sciences, Inc. Non-homogeneous systems for the resolution of enantiomeric mixtures
GB9823334D0 (en) 1998-10-23 1998-12-23 Darwin Discovery Ltd Enzyme coupling
WO2002070646A2 (fr) * 2001-02-16 2002-09-12 Ibex Technologies Cristaux d'enzymes à stabilisation matricielle et modalités d'utilisation
US7241272B2 (en) 2001-11-13 2007-07-10 Baxter International Inc. Method and composition for removing uremic toxins in dialysis processes
DK1699921T3 (da) * 2004-01-01 2010-02-01 Council Scient Ind Res Fremgangsmåde til fremstilling af tværbundne proteinkrystaller
AU2005227090B2 (en) 2004-03-22 2010-12-09 Abbott Laboratories Gmbh Oral pharmaceutical compositions of lipase-containing products, in particular of pancreatin, containing surfactants
PT2198880T (pt) 2004-10-14 2017-01-04 Lilly Co Eli Composições contendo lipase, protease e amilase para o tratamento de insuficiência pancreática
UA93384C2 (ru) 2005-07-29 2011-02-10 Солвей Фармасьютикалс Гмбх Способ получения стерилизированного порошкообразного панкреатина
US11266607B2 (en) 2005-08-15 2022-03-08 AbbVie Pharmaceuticals GmbH Process for the manufacture and use of pancreatin micropellet cores
US9198871B2 (en) 2005-08-15 2015-12-01 Abbott Products Gmbh Delayed release pancreatin compositions
US10072256B2 (en) 2006-05-22 2018-09-11 Abbott Products Gmbh Process for separating and determining the viral load in a pancreatin sample
WO2010134642A1 (fr) 2009-05-22 2010-11-25 サンヨー食品株式会社 Complexe protéique à oxydation asymétrique et son procédé de préparation
CN102753570B (zh) * 2009-09-15 2016-01-20 阿尔泰亚科技公司 A蛋白晶体和交联晶体及其使用方法
JP5740754B2 (ja) * 2010-08-26 2015-07-01 アイシン精機株式会社 酵素結晶固定化電極及び酵素結晶固定化電極の製造方法、並びに酵素結晶固定化電極を備えるバイオ電池及びバイオセンサー
US11077238B2 (en) 2013-06-07 2021-08-03 Allena Pharmaceuticals, Inc. Compositions, methods, and devices for dialysis
US20180028454A1 (en) 2015-02-04 2018-02-01 Abbvie Inc. Pharmaceutical compositions and methods of use thereof to treat pancreatic enzyme insufficiency
US20210299234A1 (en) * 2017-06-15 2021-09-30 The University Of Chicago Methods and compositions for treating cancer
WO2023194615A1 (fr) * 2022-04-07 2023-10-12 Need4Health B.V. Procédé pour déterminer le dosage d'insuline

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3664926A (en) 1970-02-26 1972-05-23 Merck & Co Inc Crystallization of l-asparaginase
DE2801238C2 (de) 1977-01-27 1986-06-05 (Zaidanhojin) Sagami Chemical Research Center, Tokio/Tokyo Verfahren zur Herstellung von Salzen aus L,L-Dipeptiden und Phenylalaninestern
US4373023A (en) * 1980-10-14 1983-02-08 Massachusetts Institute Of Technology Process for neutralizing heparin
EP0092829B1 (fr) * 1982-04-23 1990-11-07 Wako Pure Chemical Industries, Ltd. Procédé pour la semi-synthèse d'insuline humaine et protéase alcaline pour sa mise en oeuvre
US4411996A (en) * 1982-06-30 1983-10-25 Nabisco Brands, Inc. Process for isomerizing glucose
WO1985003247A1 (fr) * 1984-01-20 1985-08-01 Eye Products Limited Partnership Procede de nettoyage de lentilles de contact
FR2566798B1 (fr) * 1984-06-27 1986-12-12 Centre Nat Rech Scient Membrane porteuse de la luciferase pour le dosage de l'atp et son procede de production
US4650758A (en) * 1984-09-21 1987-03-17 Cetus Corporation Stabilization of enzymes useful in the production of glucosone and other enzymatic processes
JPS6317691A (ja) * 1986-07-09 1988-01-25 Agency Of Ind Science & Technol アスパルテ−ム製造用固定化酵素
FI85285C (fi) * 1988-05-13 1992-03-25 Stabra Ag Tvaerbundet, vattenoloesligt glukosisomeras och foerfarande foer framstaellning daerav.

Also Published As

Publication number Publication date
NO930360L (no) 1993-04-02
DE69133258D1 (de) 2003-06-18
ES2263187T3 (es) 2006-12-01
LT3366B (en) 1995-07-25
JPH05508549A (ja) 1993-12-02
IE20040113A1 (en) 2004-05-05
SK5993A3 (en) 1993-07-07
SG70966A1 (en) 2000-03-21
FI120099B (fi) 2009-06-30
EP0861888A1 (fr) 1998-09-02
ATE323158T1 (de) 2006-04-15
IL99046A0 (en) 1992-07-15
HU9300275D0 (en) 1993-04-28
ES2199933T3 (es) 2004-03-01
ATE240391T1 (de) 2003-05-15
HK1023362A1 (en) 2000-09-08
CA2087730A1 (fr) 1992-02-04
IL99046A (en) 1998-09-24
IE912767A1 (en) 1992-02-12
FI930454A0 (fi) 1993-02-02
PT98564B (pt) 1999-01-29
CA2087730C (fr) 1999-07-20
DK0861888T3 (da) 2006-08-14
DE69133521T2 (de) 2006-12-07
DE69133521D1 (de) 2006-05-24
EP0550450B1 (fr) 2003-05-14
HK1012411A1 (en) 1999-07-30
WO1992002617A1 (fr) 1992-02-20
MD1592B2 (ro) 2001-01-31
DK0550450T3 (da) 2003-09-08
FI930454A (fi) 1993-02-02
JP2004000230A (ja) 2004-01-08
CZ12393A3 (en) 1993-10-13
DE69133258T2 (de) 2004-03-18
TJ210R3 (en) 1998-11-14
NO930360D0 (no) 1993-02-02
LTIP1077A (en) 1995-04-25
AU8315491A (en) 1992-03-02
AU662104B2 (en) 1995-08-24
LV10304A (lv) 1994-10-20
PT98564A (pt) 1992-06-30
RU2124052C1 (ru) 1998-12-27
HUT65904A (en) 1994-07-28
UA27035C2 (uk) 2000-02-28
EP0550450A1 (fr) 1993-07-14
EP0861888B1 (fr) 2006-04-12
SK283185B6 (sk) 2003-03-04
HU211521A9 (en) 1995-11-28
BR9106732A (pt) 1993-08-17

Similar Documents

Publication Publication Date Title
US5618710A (en) Crosslinked enzyme crystals
EP0861888B1 (fr) Emploi de cristaux réticulés comme nouvelle forme d'immobilisation d'enzymes
IE83874B1 (en) Use of crosslinked crystals as a novel form of enzyme immobilization
Broun [20] Chemically aggregated enzymes
EP2776562B1 (fr) Enzymes biodégradables immobilisées et leurs procédés de fabrication
Mascini et al. Clinical uses of enzyme electrode probes
Crumbliss et al. Preparation and activity of carbonic anhydrase immobilized on porous silica beads and graphite rods
US5801022A (en) Method of producing a product with crosslinked crystals of thermolysin
Kauffmann et al. Enzyme electrode biosensors: theory and applications
Guilbault Immobilized enzymes as analytical reagents
Everse et al. Immobilized enzymes in biochemical analysis
IE85134B1 (en) Use of Crosslinked Crystals as a Novel Form of Enzyme Immobilization
NZ264042A (en) Crosslinked crystalline immobilized protein, biosensor containing the crosslinked protein crystal
Rai et al. Production of D-amino acids using immobilized D-hydantoinase from lentil, Lens esculenta, seeds
Hashimoto et al. Enzyme electrodes using ultrafiltration membranes as enzyme carriers
LIU ISOLATION, PURIFICATION, AND CHARACTERIZATION OF ESCHERICHIA COLI L-ASPARAGINASE