LU87135A1 - METHOD FOR INCREASING THE EFFECTIVENESS OF VEGETATIVE PROPAGATION OF POTATOES IN VITRO - Google Patents

Description

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Process for increasing vegetative propagation by jSff'yktivittit. Potatoes in vitro

Pie lärfindung relates to a process to increase the affectivity of vegetative propagation of potatoes in vitro,

The vegetative propagation of potatoes in vitro has been reported in zuAlreianeu publications. For example, a complex burning process was worked out, made with U00 VttrmeiU'ungsmatöi'iäl from kartoffoln in an operational scale and, depending on the existing requirements, can be shared out as rooted cuttings, as mini-balls or as small tubers (Hungarian patent application Mr ♦ 116u / o4) * nex the experiments in kuseiamenhang with this invention was observed. since £ the cultures in liquid Müiirmedium propagated much sunnier than on solid Agarnaiirboden, Üia dxeae advantageous effect of the r ♦ - 2 -

Kultur auMiiur anaere swecke d "to use the so-called rooted culture", attempts were made to further increase the culture of vurgenowmoii grown in liquid nutrient medium. In the course of these preliminary tests, a method was developed with which propagation in vitro was considerably more effective can be carried out as hitherto · Vû of the method consists in the fact that the culture of cultivating BtecJclingen or juiuikuollen ficrgoatalltc bowuraolte culture is further increased, namely, by cultivating the culture in troughs and the rooted part of the root with root and root parts separately on fresh nutrient medium. i) The further increase of the trieoe grown in the liquid hdhriuedium also improves the efficiency of the increase, the use of the rooted part of the ball for increasing the wuiter is an essential new element of the process, with which the increase with significant labor saving up to the i \ inf-facne be increased man *

According to one embodiment of the method according to the invention, both the shoots and the rooted hasis portion are transferred to liquid oil medium. The cultures developing from the shoots correspond entirely to the original tmwurzeltan culture, from which either mini-tuber cultures can be created in vitro, or they can be planted out in the Gewkansnaus, but they are again mixed with the described boise. Cultures grown here from the rooted Baaistell are most advantageously used for the production of kinicolin cultures in vitro.

According to another embodiment, only ui triobo are added to liquid cultivation medium and used for rooted cultures, while the rooted base part is planted with solid agax'iJtäux'UodeH wix-d, 'u aiuu with .Ulfe w - 3 - of the already voi'huhcLenen Within a short period of time, roots of the pruned shoots develop into a large, new, new plant, which are also suitable for wide proliferation. However, rooted zjeeisteil increased on firm soil, but is not suitable for propagation in vitro, but can - among other things he is equivalent to the culture grown in liquid medium - auaa are planted out in the Gewaonshaus «

According to a further »variant of the successful process, both the shoots and the rooted buais-Itsil are transferred to i'öuton agar soil. Within a short time, a large number of new shoots emerge from the rooted Baai part, while the aul de "solid breeding ground auigebraaii - ceu shoots develop roots that are sensitive to jealousy" envious cultures can be used in the already warmed up way, ie they are boldly advanced in vitro * never dilting of ω-tubers can be induced in vitro, and finally it is possible, the cultures in a greenhouse euozupi'lahzen »the variant described here is preferred, because - compared to * the cultures in liquid« jjanrmediums -uer requires less reluctantly «k-urial and the l'rieb-etdeie» νίη · 7.Ητ · Can be cut * well they develop an instinct from every eye.

The rooted culture grown on the igarn crumble can be planted together with the adhering culture medium into the «αο «when examining the fields of application. * ies aes * even» the soil has been stuck out, except for the solid culture medium which is also used in a thinner oiling medium «la uoi are n «nnj splint» of about 1 cu thickness are sufficient, root structure of the cultures grown on such.nutrition is both their propagation in vitro as well as cheaper for ia 3 plucking out in kroud * hie arceiuazel wemaiierudiutiut bi ch from two TatnnfihHtti - 4 - r 1, Bas Urunan «ter lu fliiafligco htihrm * <iiüm Pi ngftbraûh-tett 'Jînebe takes considerably less time to arrange l'rie ben aur xestem Höhi-dudu«.

2 * Ber oewar »ulte nasis part can be placed on the surface of the solid agaraahn iodine« with a single movement «, because the whole culture is brought together through the coherent original work ·

The process is explained in more detail below with the aid of exemplary embodiments. In the examples, the following method was used * KU ^ ilO .. lbOü uig Mezoinosit 100 & ë KHO-j 1900 mg vitamin 0.5 "

OaClg.ai ^ O 440 mg vitamin -M mg

MgSü ^ .ïiigO 370 "g nicotine acid 5.0 Wg

Ha ^ -EDi’A + PeSO ^? n ^ O 25 mg Panthoteaeaure 2.5 ne ü.jBO ^ c, 2 mg KaA 0.001 mg iaUBO ^ .4 ^ 0 22.3 Λtlg öacuuarvee 30 g

Znö04.4li20 ö, o ms Agar b, 5 g KI 0.0? mg pd Vf

WagiioO ^ .ÊHgO 0.25 mg

CuÖ04.5H2Ü 0.023 wg

Ouöl ^ .bHuü 0.025 mg c t

Bas liquid Hanrmediuw natte the same zuaatauieiiseiiZuiiÄ, only without agar.

For example, let's say that a potato culture grown on a solid soil has not been removed. Approximately 10 pieces were placed in 20 ml of liquid nutritional medium placed in a 400 ml volume kinkoon jar and the culture was held for about 2 weeks at 22 υυ and 3000 lux lighting turned on for 1 hour per day. During this time the culture formed 20-23 shoots and a coherent culture of Wux-AbI * Bi * was removed from the jar with tweezers under sterile conditions, the pacemakers were removed and - 5 - r in 2-year gliisôîïl i'risoüem Mahrwedium distributed, while the rooted hasisi-hurry was grasped with the tweezers and put into the liquid medium. «In this way, cultures cultivated in this way were not stimulated and the formation of minueno 1 oils After 2 * 0 months, an average of 50 parakeets could be harvested. The cultures waxed from 4exi-grown shoots could also be used for further use in the manner described, 1% · * “” * plant was planted out in part, and another part was used for the production of ulnium-fast. With the death of the method described here, more and more cultures can be added within the same period, in the case of the use of aui ’fastfita ffôhï’b <? The grown cultures.

baisuiel 2 ± is was worked in the manner described in hot game 1 with the ühtersenied that the rooted base part obtained in sterile cultures was put in separate Ag bxn knr bo à en in the liquid media. Cultures that have grown have been used to multiply, to help. June Y9ft. Felnik hollows or used to transplant into the Öewacnshau, from the rooted basisuoll, which had settled in solid nutrient medium, developed vigorous shoots within 2 weeks, which were suitable for planting further J-4 cultures. Jüit of this Metnoae could be »divided within the same about five times as many cultures as in / all of the use of cultures grown on solid nutrient media.

Claims (3)

1. A method to increase the efficiency of vegetative avoidance of potatoes in vitro, that can be done because the liquid, cultivated root-grown cultures are divided into lovable and rooted nasisteii, and these are separated separately from one another. 2. Veron clay according to claim 1, characterized in that both the resistance of the ix-ieBc as well as the continuation of the rooted hu sis partly takes place in liquid hunrweaium * Burning according to l 3pruon 1, and due to this, also the wheat curding of the i'riebe in liquid huiuiwdium, the Weitex propagation of a rooted base part takes place on firm nouveau. 4 * Procedure »ach Claim 1, marked daduren, wet suwonl the further multiplication is free wxo aucn aie further multiplication of the rooted haexa takes place partly on solid nutrient medium. h * proceed «» een oinew of h / ispruehe 1-4, known by the fact that repetitions are repeated several times * b. Method according to one of claims 1-i una 5, thereby gujtenua tienne t, that the cultures obtained from the crops grown in liquid multi-medium are used for the production of useful and / or »further spreading and / or a to plan out ins (iewuchanaua . 7, Yerfanren according to claim 1 uuw Ë, dururou characterized in that dxe from the cultures grown in liquid nutrient medium are used for the production of Liniknoilen * Λ * f * i -Τα, method, beil one of the Anaprücne 1 * 3 or 4, characterized by the fact that they can be used on solid soil v eratebrtexi (Brxeba and / or the rooted wet part to create new crops and / or for the extraction of juni bulbs in vitru and / or to the Auaplianaen ma ôoWKeàaaoufl.