GB2201572A - Process for in vitro propagation of potato - Google Patents
Process for in vitro propagation of potato Download PDFInfo
- Publication number
- GB2201572A GB2201572A GB08803408A GB8803408A GB2201572A GB 2201572 A GB2201572 A GB 2201572A GB 08803408 A GB08803408 A GB 08803408A GB 8803408 A GB8803408 A GB 8803408A GB 2201572 A GB2201572 A GB 2201572A
- Authority
- GB
- United Kingdom
- Prior art keywords
- media
- shoots
- pieces
- cultures
- rooted
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Developmental Biology & Embryology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Hydroponics (AREA)
- Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)
Description
Process for the increase the efficiency of in vitro vegetative propagation of potato.
The invention relates to a process for increase the efficiency of in vitro phase of vegetative propagation of potato.
Several methods have been developed for the in vitro vegetative propagation of potato. We have worked out a complex method which can be applied for the economical mass-production of the potato 'a propagation material in great quantities and it is suitable for the production of different propagation materials (rooted cuttings, minitubers and small tubers, No. of Hungarian patent appl.: 1160/54.).
During the application of the above mentioned invention we have found that shoots are growing and multiplying better in liquid media than on solid media.
Propagation of shoots in liquid media is preferable for getting rooted plants. In addition to this we have planned experiments for the further propagation of the rooted plants propagated by liquid culture. As a result of these experiments we. have worked out such a method which is much more efficient for the in vitro propagation compared with the earlier ones.
The essential point of this method is that the rooted plants produced in liquid media, for further cultivation in glasshouses or for production of in vitro minitubers, are propagated in such a, way that those are divided for shoots and rooted shoot-pieces at first and both parts - the shoots and rooted shoot-pieces - are placed on fresh nutritive media. The further propagation of shoots grown in liquid media is increasing the efficiency of propagation, but the essential new step of this method is the use of the rooted shootpieces for further propagation and this step can increase five times the rate of multiplication and next to this, one can spare a lot of time.
According to a variant of this invention both - the shoots and the rooted shoot-pieces - are placed in liquid media so cultures grown from shoots are identical to the original rooted cultures and those can be used for minituber production or can be~,placed into glasshouses or can be propagated on the above written way, while cultures grown from the rooted shoot-pieces can be used preferably for foundation of in vitro minituber cultures.
According to another variant of this invention only the shoots are placed in liquid media and grown further to get rooted cultures, while the rooted shoot-pieces are placed on solid media and within a short time the rooted pieces are producing several new shoots which are good for further propagation.
The rooted shot-pieces cultivated on solid agar media are suitable not only for in vitro propagation, but also for green-house planting since they are equivalent to the cultures grown on liquid media.
According to a further variant of the process both the rooted shoot-pieces and the shoots were placed onto solid media. From the rooted shoot-pieces great amount of new shoots were developed while the shoots placed onto solid media has grown uninterrupted root-system. Both of the cultures may be used as described above, i.e. may be further propagated under in vitro conditions, in vitro minitubers maybfnduced on them, and may be planted into green-house.
This last mentioned process is the preferred variant of our invention since in this process less propagation material is necessary than in the process using liquid media, and shoots may be developed from all buds of the shoot-pieces, which may be cut short.
The rooted culture grown on agar media may be planted into the soil together with the media.
During the examination of the utilization possibilities of the solid agar media it has been established that the solid media may be used also in thinner layer than usual, about in a layer of 1 cm.
The root-system of the cultures grown in such media is more favourable in respect of both the in vitro propagation and planting into the soil.
The time-spareing is the result of two factors: 1. The arrangementef shoots in liquid media needs less time compared with the case of the solid media.
2. The rooted shoot-pieces can be placed on to the fresh liquid or solid media with one movement because of the adjacent roots.
The following examples illustrate the invention.
The composition of the nutritive media used by us is the
following:
Potato nutritive media
Solid, maintaining and propagating media:
NH4N03 1600 mg Mesoinosit 100 mg
KN03 1900 mg Thiamine HCl 0,5 mg
CaCl2.2H20 440 mg Pyridoxine HCl 1,0 mg
MgS04.7H20 370 mg Nicotinic acid 5,0 mg
Na2-EDTA+FeS04.7H20 25 mg Panthotene acid 2,5 mg H3604 6,2 mg Naphtylacetic acid 0,OOlmg MnS04.4H20 22,3 mg Sacharose 30 g
ZnS04.4H20 8,6 mg Agar 6,5 g
KI 0,83 mg pH 5,7
Na2Mo04.2H20 0,25 mg
CuS04.5H20 0,025 mg CoCl2.6H20 0,025 mg
Liquid rooting media: as above, without agar.
Example 1.
The shoots of the potato cultures grown on solid media were cut and approx. 10 pieces of it were placed in a 400 ml flask containing 20 ml liquid media and were cultured approx. for two weeks under 22 0C 16 hours 3000 lux illumination. Under these conditions 20-25 new shoots and adjacent roots are developing. The rooted culture is removed with a forceps from the flask under steril condition, the shoots are cut and placed into 2-3 flasks containing fresh nutritive media and the remaining rooted shoot- pieces are placed in a new liquid media. Cultures prepared in this way were grown for 2 weeks and were induced for minituber production and after 2-3 months - in average - 50 mini tubers can be produced by them.
Cultures developed from the cutted shoots can be used - on the above written way - for propagation, can be transplanted into glasshouses or minitubers can be induced by them.
Using the process detailed above one can produce 50% more cultures within the same time as one uses cultures grown on solid media.
Example 2.
Shoots of cultures grown on solid media (the composition of it is written above) were cut and approx. 10 pieces of it were placed into a 400 ml flask containing 20 ml liquid media and were cultured approx. for 2 weeks under 22 C 16 hours 3000 lux illumination. Under these conditions cultures were developing 20-20 new shoots and adjacent roots. The rooted cultures were removed with steril forceps, shoots were cut and placed into 2-3 flasks containing fresh liquid media. Cultures grown in liquid media were used for further propagation, minituber induction or were planted out into glasshouses. The rooted shoot-pieces placed on solid media were developing new shoots within 2 weeks, which are suitable for foundation of 3-4 new cultures.
Using the process detailed above one can produce 5 times more cultures within the same period as if one is only using cultures grown on solid media for propagation and a lot of time can be spared.
Claims (8)
1. A process for the increase the efficiency of in vitro vegetative propagation of potato, characterized in that, the rooted cultures grown in liquid media can be propagated fully by dividing them for shoots and rooted shoot -pieces.
2. The process as claimed in claim 1, characterized in that, both parts - the shoots and the rooted shoot-pieces are propagated further in liquid media.
3. The process as claimed in claim 1, characterized in that, the shoots are propagated further in liquid media, the rooted shoot-pieces are propagated further on solid media.
4. The process as claimed in claim 1, characterized in that both the shoots and the rooted shoot-pieces are propagated on solid agar media.
5. The process as claimed in any one of claims 1 to 4, characterized in that, the process can be repeated several times.
6. The process as claimed in any one of claims 1 to 3 and 5 characterized in that, cultures developed from shoots grown in liquid media can be used for minituber induction and/or further propagation and/or planting into glasshouses.
7. The process as claimed in claim 1 or 2, characteriz in that, cultures developed from rooted shoot-pieces grown in liquid media can be used for minituber induction.
8. The process as claimed in claim 1 or 3 or 4, characterized in that, cultures developed from rooted shoot -pieces grown on solid media can be used for propagation of new cultures.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| HU87595A HU204946B (en) | 1987-02-16 | 1987-02-16 | Method for increasing the effectiveness of "in vitro" vegetative propagation of potato |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| GB8803408D0 GB8803408D0 (en) | 1988-03-16 |
| GB2201572A true GB2201572A (en) | 1988-09-07 |
Family
ID=10950343
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB08803408A Withdrawn GB2201572A (en) | 1987-02-16 | 1988-02-15 | Process for in vitro propagation of potato |
Country Status (21)
| Country | Link |
|---|---|
| JP (1) | JPS63279723A (en) |
| CN (1) | CN1032448A (en) |
| AU (1) | AU1173188A (en) |
| BE (1) | BE1000851A4 (en) |
| DD (1) | DD267652A5 (en) |
| DE (1) | DE3804702A1 (en) |
| DK (1) | DK76488A (en) |
| FI (1) | FI880681A (en) |
| FR (1) | FR2610785A1 (en) |
| GB (1) | GB2201572A (en) |
| GR (1) | GR880100088A (en) |
| HU (1) | HU204946B (en) |
| IT (1) | IT8819410A0 (en) |
| LU (1) | LU87135A1 (en) |
| NL (1) | NL8800376A (en) |
| NO (1) | NO880663L (en) |
| PL (1) | PL270676A1 (en) |
| PT (1) | PT86764B (en) |
| SE (1) | SE8800528L (en) |
| TR (1) | TR23306A (en) |
| YU (1) | YU30288A (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5047343A (en) * | 1988-04-29 | 1991-09-10 | Wisconsin Alumni Research Foundation | Microtuber propagation of potatoes |
| KR920001196B1 (en) * | 1989-03-11 | 1992-02-06 | 한국과학기술원 | Propagation of potato by microtuber using petridish |
| CN102257957B (en) * | 2011-05-17 | 2012-12-19 | 北方民族大学 | Early-maturing potato detoxification basic seedling culture medium and preparation method thereof |
| CN106105639A (en) * | 2016-06-27 | 2016-11-16 | 安徽梅兰园林景观工程有限公司 | A kind of Rhizoma Solani tuber osi implantation methods |
| CN109380114A (en) * | 2018-11-06 | 2019-02-26 | 延边大学 | A kind of method of fast-propagation potato test tube seedling |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4569914A (en) * | 1982-06-28 | 1986-02-11 | Gyogynoveny Kutato Intezet | Process for the sterile micropropagation of plant material |
| EP0233492A1 (en) * | 1986-01-29 | 1987-08-26 | Cpc International Inc. | Process for microplant propagation through increased multiple shoot formation |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR886424A (en) * | 1941-04-25 | 1943-10-14 | Process for the improvement, multiplication or cultivation of potatoes |
-
1987
- 1987-02-16 HU HU87595A patent/HU204946B/en not_active IP Right Cessation
-
1988
- 1988-02-12 FI FI880681A patent/FI880681A/en not_active Application Discontinuation
- 1988-02-15 DE DE3804702A patent/DE3804702A1/en not_active Withdrawn
- 1988-02-15 DD DD88312926A patent/DD267652A5/en unknown
- 1988-02-15 NO NO88880663A patent/NO880663L/en unknown
- 1988-02-15 DK DK076488A patent/DK76488A/en not_active Application Discontinuation
- 1988-02-15 PT PT86764A patent/PT86764B/en not_active IP Right Cessation
- 1988-02-15 FR FR8801753A patent/FR2610785A1/en not_active Withdrawn
- 1988-02-15 IT IT8819410A patent/IT8819410A0/en unknown
- 1988-02-15 GB GB08803408A patent/GB2201572A/en not_active Withdrawn
- 1988-02-16 BE BE8800184A patent/BE1000851A4/en not_active IP Right Cessation
- 1988-02-16 AU AU11731/88A patent/AU1173188A/en not_active Abandoned
- 1988-02-16 YU YU00302/88A patent/YU30288A/en unknown
- 1988-02-16 LU LU87135A patent/LU87135A1/en unknown
- 1988-02-16 JP JP63034942A patent/JPS63279723A/en active Pending
- 1988-02-16 GR GR880100088A patent/GR880100088A/en unknown
- 1988-02-16 SE SE8800528A patent/SE8800528L/en not_active Application Discontinuation
- 1988-02-16 PL PL27067688A patent/PL270676A1/en unknown
- 1988-02-16 TR TR93/88A patent/TR23306A/en unknown
- 1988-02-16 NL NL8800376A patent/NL8800376A/en not_active Application Discontinuation
- 1988-02-16 CN CN88101471.0A patent/CN1032448A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4569914A (en) * | 1982-06-28 | 1986-02-11 | Gyogynoveny Kutato Intezet | Process for the sterile micropropagation of plant material |
| EP0233492A1 (en) * | 1986-01-29 | 1987-08-26 | Cpc International Inc. | Process for microplant propagation through increased multiple shoot formation |
Non-Patent Citations (1)
| Title |
|---|
| WO A1 88/02213 * |
Also Published As
| Publication number | Publication date |
|---|---|
| SE8800528L (en) | 1988-08-17 |
| NO880663L (en) | 1988-08-17 |
| SE8800528D0 (en) | 1988-02-16 |
| PL270676A1 (en) | 1988-12-08 |
| JPS63279723A (en) | 1988-11-16 |
| AU1173188A (en) | 1988-08-18 |
| DK76488A (en) | 1988-08-17 |
| DE3804702A1 (en) | 1988-08-25 |
| NL8800376A (en) | 1988-09-16 |
| DD267652A5 (en) | 1989-05-10 |
| FR2610785A1 (en) | 1988-08-19 |
| IT8819410A0 (en) | 1988-02-15 |
| DK76488D0 (en) | 1988-02-15 |
| CN1032448A (en) | 1989-04-19 |
| HUT46353A (en) | 1988-10-28 |
| HU204946B (en) | 1992-03-30 |
| GB8803408D0 (en) | 1988-03-16 |
| TR23306A (en) | 1989-09-19 |
| GR880100088A (en) | 1988-12-16 |
| FI880681A0 (en) | 1988-02-12 |
| NO880663D0 (en) | 1988-02-15 |
| PT86764B (en) | 1992-04-30 |
| YU30288A (en) | 1989-06-30 |
| FI880681A (en) | 1988-08-17 |
| LU87135A1 (en) | 1989-09-20 |
| PT86764A (en) | 1988-03-01 |
| BE1000851A4 (en) | 1989-04-18 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| WAP | Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1) |