CN102257957B - Early-maturing potato detoxification basic seedling culture medium and preparation method thereof - Google Patents
Early-maturing potato detoxification basic seedling culture medium and preparation method thereof Download PDFInfo
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Abstract
The invention relates to an early-maturing potato detoxification basic seedling culture medium and a preparation method thereof. The preparation method is characterized by comprising the following steps of: (1) preparing the basic mother liquor of the culture medium, preparing macroelement mother liquor, preparing microelement mother liquor, preparing ferric salt mother liquor, preparing No.1 organic matter mother liquor, and respectively measuring 100ml of macroelement mother liquor, 100mL of ferric salt mother liquor, 10ml of microelement mother liquor and 10ml of No.1 organic matter motherliquor for per liter of culture medium; (2) adding 5g of agar powder to per liter of culture medium, adding 200ml of distilled water to per g of agar powder; (3) adding C source and mixed mother liquor in a beaker into boiled agar powder; (4) regulating a pH value to 5.8-6.0 with sodium hydroxide or hydrochloric acid; and (5) bottling and then autoclaving. In the culture medium provided by the invention, nutrient components are reduced, and seedling in a test tube grows vigorously and period is shortened, thus energy consumption and cost during seedling are reduced.
Description
Technical field
The present invention relates to a kind of precocious potato detoxicating basis seedling medium and preparation method thereof.
Background technology
It is to produce potato detoxicating basis seedling through group culturation rapid propagating technology that China's virus-free potato primary stock industrialization is at present produced, and again it is transplanted the refining seedling and produces micro potato.And the MS medium is the medium of the numerous extensive employing of present potato detoxicating basis seedling industrialization the expansion, and its agar consumption is too high, have in addition to 1.7%, basic seedling is grown under the matrix environment really up to the mark; Crisp flexibility of stem is relatively poor, causes basic seedling growing way poor, and a basic seedling and propagating generation needs about 25 days; Before transplanting the refining seedling, from bottle, take out basic seedling, pour in the warm water; Agar around the root is washed off, and the root easy damaged influenced transplanting survival rate when agar was washed root more firmly.Do not select medium for use, strengthened industrial production cost, make basic seedling growth potential relatively poor by the kind maturing stage.Between culture period, increased cost of drugs and used electrical loss, and basic seedling growth potential is relatively poor, the poor quality, unfavorable to suitability for industrialized production.
Summary of the invention
One of the object of the invention provides a kind of precocious potato detoxicating basis seedling medium, adopts the detoxification basis seedling of this medium with short production cycle, growth is vigorous, production cost is low.
Two of the object of the invention provides the preparation method of a kind of above-mentioned precocious potato detoxicating basis seedling medium.
For realizing above-mentioned purpose, the technical scheme that the present invention adopts is.
A kind of precocious potato detoxicating basis seedling medium, its special feature is, calculates with the 1L medium and contains: the KNO of 1890～1910mg
3, 169～171mg KH
2PO
4, 369～371mg MgSO
47H
2The CaCl of O, 439～441mg
22H
2The MnSO of O, 22.27～22.23mg
44H
2The KI of O, 0.827～0.833mg, the H of 6.17～6.23mg
3BO
3, 8.57～8.63mg ZnSO
47H
2The Na of O, 0.247～0.253mg
2MoO
4H
2The CuSO of O, 0.0247～0.0253mg
45H
2The CoCl of O, 0.0247～0.0253mg
26H
2The Na of O, 37.0～37.3mg
2The FeSO of EDTA, 26～28mg
47H
2The VB of O, 1.99～2.01mg glycine, 0.09～0.11mg
1, 0.49～0.51mg VB
6, and the VB of 0.49～0.51mg
5Or VPP; Surplus is a water.
The inositol that wherein also contains 24.9～25.1mg.
The NH that wherein also contains 815～835mg
4NO, the C source sucrose of 14900～15100mg.
The preparation method of a kind of precocious potato detoxicating basis seedling medium, its special feature is, comprises the steps:
(1) preparation of the basic mother liquor of medium
A, preparation macroelement mother liquor
Get the KNO of 18900～19100mg
3, 1690～1710mg KH
2PO
4, 3690～3710mg MgSO
47H
2The CaCl of O and 4390～4410mg
22H
2O, more than all kinds of materials fully dissolve with distilled water respectively, put in order by it and pour constant volume in the 1L volumetric flask successively into, it is subsequent use to reinstall in the reagent bottle that posts the concentration label 4 ℃ of preservations;
B, prepare micro-mother liquor
Get the MnSO of 2227～2223mg
44H
2The KI of O, 82.7～83.3mg, the H of 617～623mg
3BO
3, 857～863mg ZnSO
47H
2The Na of O, 24.7～25.3mg
2MoO
4H
2The CuSO of O, 2.47～2.53mg
45H
2The CoCl of O and 2.47～2.7mg
26H
2O, more than all kinds of materials fully dissolve with distilled water respectively, constant volume is in the 1L volumetric flask, it is subsequent use to reinstall in the reagent bottle that posts the concentration label 4 ℃ of preservations;
C, preparation mother liquid of iron salt
Get the Na of 370～373mg
2The FeSO of EDTA and 260～280mg
47H
2O, more than all kinds of materials fully dissolve with distilled water respectively, constant volume is in the 1L volumetric flask, it is subsequent use to reinstall in the reagent bottle that posts the concentration label 4 ℃ of preservations;
D, No. 1 organic matter mother liquor of preparation
Get the VB of 199～201mg glycine, 9～11mg
1, 49～51mg VB
6, and the VB of 49～51mg
5Or VPP, more than all kinds of materials fully dissolve with distilled water respectively, constant volume is in the 1L volumetric flask, it is subsequent use to reinstall in the reagent bottle that posts the concentration label 4 ℃ of preservations;
Measure 100mL macroelement mother liquor, mother liquid of iron salt respectively by every liter of medium, and 10mL trace element mother liquor and No. 1 organic matter mother liquor, it is subsequent use to put into beaker;
(2) add 5 grams by every liter of medium and take by weighing agar powder, add 200ml distilled water, add and boil, stop heating then to as clear as crystal by every gram agar powder;
(3) the mixing mother liquor in the beaker, C source are added in the well-done agar powder, add water again and make culture medium C source quality concentration reach 1.49～1.51%, the agar powder mass concentration reaches 0.5%, heats 80-121 ℃ and stirs;
(4) with sodium hydroxide or hydrochloric acid the pH value is transferred to 5.8～6.0, promptly accomplishes the preparation of solid culture medium;
(5) bottling back autoclaving gets final product.
The NH that wherein in the macroelement mother liquor, also adds 8150～8350mg
4NO
3
Wherein in the preparation of the basic mother liquor of medium, also will prepare No. 2 the organic matter mother liquor, specifically get the inositol of 249～251mg, fully dissolve with distilled water, constant volume is in the 1L volumetric flask, and it is subsequent use to reinstall in the reagent bottle that posts the concentration label 4 ℃ of preservations; And measure 100mL macroelement mother liquor, mother liquid of iron salt and No. 2 organic matter mother liquors respectively by every liter of medium, and 10mL trace element mother liquor and No. 1 organic matter mother liquor, it is subsequent use to put into beaker.
Wherein regulating the pH value is sodium hydroxide or the hydrochloric acid with 1mol/L, splashes in the step prepared culture medium, stirs after dripping, and repeats this process and is transferred to 5.8～6.0 until the pH value, promptly accomplishes the preparation of solid culture medium.
Wherein bottling back autoclaving is with the solid culture medium for preparing, and every bottled 30～40mL seals tight bottleneck, puts into the autoclaving basket, and 121 ℃ of sterilizations are taken out after 15～19.5 minutes and got final product.
Compare with background technology, nutrient component reduces in the medium of the present invention, but test-tube plantlet growth is vigorous and the cycle shortens, thereby has effectively reduced energy consumption and the cost in growing seedlings.
Embodiment
Embodiment 1:
The precocious potato test-tube plantlet medium of detoxification components by weight percent raw material is produced as follows:
A, macroelement mother liquor (mother liquor 1) are formed: 18900～19100mg potassium nitrate (KNO
3), 8150～8350mg ammonium nitrate (NH
4NO
3), 1690～1710mg potassium dihydrogen phosphate (KH
2PO
4), 369～3710mg magnesium sulfate (MgSO
47H
2O), 4390～4410mg calcium chloride (CaCl
22H
2O), more than all kinds of materials fully dissolve with distilled water respectively, put in order by it and pour constant volume in the 1L volumetric flask successively into, it is subsequent use to reinstall in the reagent bottle that posts the concentration label 4 ℃ of preservations;
B, micro-mother liquor (mother liquor 2) are formed: the MnSO of 2227～2223mg
44H
2The KI of O, 82.7～83.3mg, the H of 617～623mg
3BO
3, 857～863mg ZnSO
47H
2The Na of O, 24.7～25.3mg
2MoO
4H
2The CuSO of O, 2.47～2.53mg
45H
2The CoCl of O and 2.47～2.7mg
26H
2O, more than all kinds of materials fully dissolve with distilled water respectively, constant volume is in the 1L volumetric flask, it is subsequent use to reinstall in the reagent bottle that posts the concentration label 4 ℃ of preservations;
C, mother liquid of iron salt are formed (mother liquor 3): 370～373mg disodium ethylene diamine tetraacetate (Na
2EDTA), 260～280mg ferrous sulfate (FeSO
47H
2O), more than all kinds of materials fully dissolve with distilled water respectively, constant volume reinstalls 4 ℃ of preservations, subsequent use in the reagent bottle that posts the concentration label in the 1L volumetric flask;
D, No. 1 organic matter mother liquor are formed (mother liquor 4): the VB of 199～201mg glycine, 9～11mg
1, 49～51mg VB
6, and the VB of 49～51mg
5Or VPP, more than all kinds of materials fully dissolve with distilled water respectively, constant volume reinstalls 4 ℃ of preservations, subsequent use in the reagent bottle that posts the concentration label in the 1L volumetric flask;
E, No. 2 organic matter mother liquors are formed (mother liquor 5): 249～251mg inositol, and above material fully dissolves with distilled water, and constant volume reinstalls 4 ℃ of preservations, subsequent use in the reagent bottle that posts the concentration label in the 1L volumetric flask;
Measure 100mL mother liquor 1, mother liquor 3, mother liquor 5 according to every liter of medium respectively, measure 10mL mother liquor 2, mother liquor 4, it is subsequent use to put into beaker:
(2) every liter of medium adds 5 gram agar powders altogether, takes by weighing agar powder with the balance of sensibility reciprocal 1%, adds 200ml distilled water by every g agar powder, boils to as clear as crystal, stops heating again;
(3) the mixing mother liquor in the beaker, C source are added in the well-done agar powder, add water again and make culture medium C source quality concentration reach 1.49～1.51%, the agar powder mass concentration reaches 0.5%, heats 80-121 ℃ and stirs;
(4) with sodium hydroxide or the hydrochloric acid of 1mol/L, splash in the step prepared culture medium, stir after dripping, repeat this process and be transferred to 5.8～6.0 until the pH value, promptly accomplish the preparation of solid culture medium;
(5) with the medium for preparing, every bottled 30～40mL seals tight bottleneck, puts into the autoclaving basket, sterilizes 15～19.5 minutes, and can accomplish the preparation of medium for 121 ℃.
On the medium of this mother liquor preparation; When cultivating 12d, mensuration potato detoxicating basis each economical character index of seedling is: individual plant seedling fresh weight 165.0mg, dry weight 10.0mg, plant height 7.86cm, the thick 1.12mm of stem, the number of blade 6.2, several 6.6 of root bar, the long 3.16cm of root.
Embodiment 2:
The difference of present embodiment and embodiment 1 is: potassium nitrate (KNO3) content is that 4750mg, ammonium nitrate (NH4NO3) are 0mg in every liter of mother liquor 1, and mother liquor 4 mysoinositol content are 0mg.
On the medium of this mother liquor preparation; When cultivating 12d, mensuration potato detoxicating basis each economical character index of seedling is: individual plant seedling fresh weight 76.0mg, dry weight 2.8mg, plant height 4.56cm, the thick 1.04mm of stem, the number of blade 5.3, several 4.5 of root bar, the long 5.92cm of root.
Embodiment 3:
The difference of present embodiment and embodiment 1 is: potassium nitrate (KNO in every liter of mother liquor 1
3) content is 4750mg, ammonium nitrate (NH
4NO
3) be 4125mg, cane sugar content is 2%.
On the medium of this mother liquor preparation; When cultivating 12d, mensuration potato detoxicating basis each economical character index of seedling is: individual plant seedling fresh weight 141.0mg, dry weight 6.8mg, plant height 5.15cm, the thick 1.02mm of stem, the number of blade 5.88, several 6.2 of root bar, the long 5.13cm of root.
Embodiment 4:
The difference of present embodiment and embodiment 1 is: potassium nitrate (KNO in every liter of mother liquor 1
3) content is 4750mg, mother liquor 4 mysoinositol content are 5000mg, cane sugar content is 2.5%.
On the medium of this mother liquor preparation; When cultivating 12d, mensuration potato detoxicating basis each economical character index of seedling is: individual plant seedling fresh weight 130.0mg, dry weight 8.7mg, plant height 4.59cm, the thick 0.9mm of stem, the number of blade 4.06, several 5.0 of root bar, the long 4.59cm of root.
Embodiment 5:
The difference of present embodiment and embodiment 1 is: potassium nitrate (KNO in every liter of mother liquor 1
3) content is 9500mg, ammonium nitrate (NH
4NO
3) be 0mg, cane sugar content is 2.5%.
On the medium of this mother liquor preparation; When cultivating 12d, mensuration potato detoxicating basis each economical character index of seedling is: individual plant seedling fresh weight 94.0mg, dry weight 7.5mg, plant height 3.11cm, the thick 0.83mm of stem, the number of blade 3.85, several 4.8 of root bar, the long 3.35cm of root.
Embodiment 6:
The difference of present embodiment and embodiment 1 is: potassium nitrate (KNO in every liter of mother liquor 1
3) content is 9500mg, ammonium nitrate (NH
4NO
3) be 4125mg, mother liquor 4 mysoinositol content are 5000mg.
On the medium of this mother liquor preparation, when cultivating 12d, mensuration potato detoxicating basis each economical character index of seedling is: individual plant seedling fresh weight 88.0mg, dry weight 4.9mg, plant height 4.7cm, the thick 1.0mm of stem, the number of blade 5.28, several 4.9 of root bar, the long 3.6cm of root.
Embodiment 7:
The difference of present embodiment and embodiment 1 is: potassium nitrate (KNO in every liter of mother liquor 1
3) content is 9500mg, mother liquor 4 mysoinositol content are 0mg, cane sugar content is 2.0%.
On the medium of this mother liquor preparation; When cultivating 12d, mensuration potato detoxicating basis each economical character index of seedling is: individual plant seedling fresh weight 140.0mg, dry weight 7.0mg, plant height 6.97cm, the thick 1.24mm of stem, the number of blade 5.93, several 6.9 of root bar, the long 3.47cm of root.
Embodiment 8:
The difference of present embodiment and embodiment 1 is: ammonium nitrate (NH in every liter of mother liquor 1
4NO
3) be 0mg, mother liquor 4 mysoinositol content are 5000mg, cane sugar content is 2.0%.
On the medium of this mother liquor preparation; When cultivating 12d, mensuration potato detoxicating basis each economical character index of seedling is: individual plant seedling fresh weight 110.0mg, dry weight 4.47mg, plant height 5.19cm, the thick 0.85mm of stem, the number of blade 6.15, several 4.4 of root bar, the long 5.21cm of root.
Embodiment 9:
The difference of present embodiment and embodiment 1 is: ammonium nitrate (NH in every liter of mother liquor 1
4NO
3) be 4125mg, mother liquor 4 mysoinositol content are 0mg, cane sugar content is 2.5%.
On the medium of this mother liquor preparation; When cultivating 12d, mensuration potato detoxicating basis each economical character index of seedling is: individual plant seedling fresh weight 165.0mg, dry weight 8.3mg, plant height 7.19cm, the thick 1.08mm of stem, the number of blade 6.0, several 4.3 of root bar, the long 3.37cm of root.
Embodiment 10:
Present embodiment (MS medium) is with the difference of embodiment 1: ammonium nitrate (NH in every liter of mother liquor 1
4NO
3) be 16500mg, mother liquor 4 mysoinositol content are 10000mg, cane sugar content is 2.5%.
On the medium of this mother liquor preparation; When cultivating 12d, mensuration potato detoxicating basis each economical character index of seedling is: individual plant seedling fresh weight 172.0mg, dry weight 9.1mg, plant height 5.06cm, the thick 1.04mm of stem, the number of blade 4.2, several 4.3 of root bar, the long 4.47cm of root.Through evidence, culture medium cost of the present invention is lower than widely used MS (CK) 14.7% in the present production.
Claims (8)
1. a precocious potato detoxicating basis seedling medium is characterized in that, calculates with the 1L medium and contains: the KNO of 1890～1910mg
3, 169～171mg KH
2PO
4, 369～371mg MgSO
47H
2The CaCl of O, 439～441mg
22H
2The MnSO of O, 22.27～22.23mg
44H
2The KI of O, 0.827～0.833mg, the H of 6.17～6.23mg
3BO
3, 8.57～8.63mg ZnSO
47H
2The Na of O, 0.247～0.253mg
2MoO
4H
2The CuS0 of O, 0.0247～0.0253mg
45H
2The CoCl of O, 0.0247～0.0253mg
26H
2The Na of O, 37.0～37.3mg
2The FeSO of EDTA, 26～28mg
47H
2The VB of O, 1.99～2.01mg glycine, 0.09～0.11mg
1, 0.49～0.51mg VB
6, and the VB of 0.49～0.51mg
5Or VPP;
Every liter of medium contains 5 gram agar powders;
Every liter of medium also contains the inositol of 24.9～25.1mg, and the NH of 815～835mg
4NO
3, the C source sucrose of 14900～15100mg, surplus is a water.
2. a precocious potato detoxicating basis seedling medium is characterized in that, calculates with the 1L medium and contains: the KNO of 1890～1910mg
3, 169～171mg KH
2PO
4, 369～371mg MgSO
47H
2The CaCl of O, 439～441mg
22H
2The MnSO of O, 22.27～22.23mg
44H
2The KI of O, 0.827～0.833mg, the H of 6.17～6.23mg
3BO
3, 8.57～8.63mg ZnSO
47H
2The Na of O, 0.247～0.253mg
2MoO
4H
2The CuS0 of O, 0.0247～0.0253mg
45H
2The CoCl of O, 0.0247～0.0253mg
26H
2The Na of O, 37.0～37.3mg
2The FeSO of EDTA, 26～28mg
47H
2The VB of O, 1.99～2.01mg glycine, 0.09～0.11mg
1, 0.49～0.51mg VB
6, and the VB of 0.49～0.51mg
5Or VPP;
Every liter of medium contains 5 gram agar powders;
Every liter of medium also contains the NH of 815～835mg
4NO
3, the C source sucrose of 14900～15100mg, surplus is a water.
3. the preparation method of a precocious potato detoxicating basis seedling medium is characterized in that, comprises the steps:
(1) preparation of the basic mother liquor of medium
A, preparation macroelement mother liquor
Get the KNO of 18900～19100mg
3, 1690～1710mg KH
2PO
4, 3690～3710mg MgSO
47H
2The CaCl of O and 4390～4410mg
22H
2O, more than all kinds of materials fully dissolve with distilled water respectively, put in order by it and pour constant volume in the 1L volumetric flask successively into, it is subsequent use to reinstall in the reagent bottle that posts the concentration label 4 ℃ of preservations; The NH that wherein in the macroelement mother liquor, also adds 8150～8350mg
4NO
3
B, prepare micro-mother liquor
Get the MnSO of 2227～2223mg
44H
2The KI of O, 82.7～83.3mg, the H of 617～623mg
3BO
3, 857～863mg ZnSO
47H
2The Na of O, 24.7～25.3mg
2MoO
4H
2The CuS0 of O, 2.47～2.53mg
45H
2The CoCl of O and 2.47～2.53mg
26H
2O, more than all kinds of materials fully dissolve with distilled water respectively, constant volume is in the 1L volumetric flask, it is subsequent use to reinstall in the reagent bottle that posts the concentration label 4 ℃ of preservations;
C, preparation mother liquid of iron salt
Get the Na of 370～373mg
2The FeSO of EDTA and 260～280mg
47H
2O, more than all kinds of materials fully dissolve with distilled water respectively, constant volume is in the 1L volumetric flask, it is subsequent use to reinstall in the reagent bottle that posts the concentration label 4 ℃ of preservations;
D, No. 1 organic matter mother liquor of preparation
Get the VB of 199～201mg glycine, 9～11mg
1, 49～51mg VB
6, and the VB of 49～51mg
5Or VPP, more than all kinds of materials fully dissolve with distilled water respectively, constant volume is in the 1L volumetric flask, it is subsequent use to reinstall in the reagent bottle that posts the concentration label 4 ℃ of preservations;
Measure 100mL macroelement mother liquor, mother liquid of iron salt respectively by every liter of medium, and 10mL trace element mother liquor and No. 1 organic matter mother liquor, it is subsequent use to put into beaker;
(2) add 5 grams by every liter of medium and take by weighing agar powder, add 200ml distilled water, add and boil, stop heating then to as clear as crystal by every gram agar powder;
(3) the mixing mother liquor in the beaker, C source are added in the well-done agar powder, add water again and make culture medium C source quality concentration reach 1.49～1.51%, the agar powder mass concentration reaches 0.5%, heats 80-121 ℃ and stirs; Wherein the C source is a sucrose;
(4) with sodium hydroxide or hydrochloric acid the pH value is transferred to 5.8～6.0, promptly accomplishes the preparation of solid culture medium;
(5) bottling back autoclaving gets final product.
4. the preparation method of precocious potato detoxicating as claimed in claim 3 basis seedling medium; It is characterized in that: wherein regulating the pH value is sodium hydroxide or the hydrochloric acid with 1mol/L; Splash in the step prepared culture medium; Stir after dripping, repeat this process and be transferred to 5.8～6.0, promptly accomplish the preparation of solid culture medium until the pH value.
5. the preparation method of precocious potato detoxicating as claimed in claim 3 basis seedling medium; It is characterized in that: wherein bottling back autoclaving is with the solid culture medium for preparing; Every bottled 30～40mL; Seal tight bottleneck, put into the autoclaving basket, 121 ℃ of sterilizations are taken out after 15～19.5 minutes and are got final product.
6. the preparation method of a precocious potato detoxicating basis seedling medium is characterized in that, comprises the steps:
(1) preparation of the basic mother liquor of medium
A, preparation macroelement mother liquor
Get the KNO of 18900～19100mg
3, 1690～1710mg KH
2PO
4, 3690～3710mg MgSO
47H
2The CaCl of O and 4390～4410mg
22H
2O, more than all kinds of materials fully dissolve with distilled water respectively, put in order by it and pour constant volume in the 1L volumetric flask successively into, it is subsequent use to reinstall in the reagent bottle that posts the concentration label 4 ℃ of preservations; The NH that wherein in the macroelement mother liquor, also adds 8150～8350mg
4NO
3
B, prepare micro-mother liquor
Get the MnSO of 2227～2223mg
44H
2The KI of O, 82.7～83.3mg, the H of 617～623mg
3BO
3, 857～863mg ZnSO
47H
2The Na of O, 24.7～25.3mg
2MoO
4H
2The CuS0 of O, 2.47～2.53mg
45H
2The CoCl of O and 2.47～2.53mg
26H
2O, more than all kinds of materials fully dissolve with distilled water respectively, constant volume is in the 1L volumetric flask, it is subsequent use to reinstall in the reagent bottle that posts the concentration label 4 ℃ of preservations;
C, preparation mother liquid of iron salt
Get the Na of 370～373mg
2The FeSO of EDTA and 260～280mg
47H
2O, more than all kinds of materials fully dissolve with distilled water respectively, constant volume is in the 1L volumetric flask, it is subsequent use to reinstall in the reagent bottle that posts the concentration label 4 ℃ of preservations;
D, No. 1 organic matter mother liquor of preparation
Get the VB of 199～201mg glycine, 9～11mg
1, 49～51mg VB
6, and the VB of 49～51mg
5Or VPP, more than all kinds of materials fully dissolve with distilled water respectively, constant volume is in the 1L volumetric flask, it is subsequent use to reinstall in the reagent bottle that posts the concentration label 4 ℃ of preservations;
Also will prepare the organic matter mother liquor No. 2, specifically get the inositol of 249～251mg, fully dissolve with distilled water, constant volume is in the 1L volumetric flask, and it is subsequent use to reinstall in the reagent bottle that posts the concentration label 4 ℃ of preservations;
And measure 100mL macroelement mother liquor, mother liquid of iron salt and No. 2 organic matter mother liquors respectively by every liter of medium, and 10mL trace element mother liquor and No. 1 organic matter mother liquor, it is subsequent use to put into beaker;
(2) add 5 grams by every liter of medium and take by weighing agar powder, add 200ml distilled water, add and boil, stop heating then to as clear as crystal by every gram agar powder;
(3) the mixing mother liquor in the beaker, C source are added in the well-done agar powder, add water again and make culture medium C source quality concentration reach 1.49～1.51%, the agar powder mass concentration reaches 0.5%, heats 80-121 ℃ and stirs; Wherein the C source is a sucrose;
(4) with sodium hydroxide or hydrochloric acid the pH value is transferred to 5.8～6.0, promptly accomplishes the preparation of solid culture medium;
(5) bottling back autoclaving gets final product.
7. the preparation method of precocious potato detoxicating as claimed in claim 6 basis seedling medium; It is characterized in that: wherein regulating the pH value is sodium hydroxide or the hydrochloric acid with l mol/L; Splash in the step prepared culture medium; Stir after dripping, repeat this process and be transferred to 5.8～6.0, promptly accomplish the preparation of solid culture medium until the pH value.
8. the preparation method of precocious potato detoxicating as claimed in claim 6 basis seedling medium; It is characterized in that: wherein bottling back autoclaving is with the solid culture medium for preparing; Every bottled 30～40mL; Seal tight bottleneck, put into the autoclaving basket, 121 ℃ of sterilizations are taken out after 15～19.5 minutes and are got final product.
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|---|---|---|---|
| CN 201110125824 CN102257957B (en) | 2011-05-17 | 2011-05-17 | Early-maturing potato detoxification basic seedling culture medium and preparation method thereof |
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| CN 201110125824 CN102257957B (en) | 2011-05-17 | 2011-05-17 | Early-maturing potato detoxification basic seedling culture medium and preparation method thereof |
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| CN102257957A CN102257957A (en) | 2011-11-30 |
| CN102257957B true CN102257957B (en) | 2012-12-19 |
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| CN102870682A (en) * | 2012-10-26 | 2013-01-16 | 山东省农业科学院蔬菜研究所 | Culture medium for in-vitro induced regeneration plants of double-haploid stems of potatoes |
| CN109924128A (en) * | 2017-12-15 | 2019-06-25 | 惠州市欣禾田现代农业有限公司 | A kind of toxicity-removing white potato tissue cultures Nutrient medium and its match Preparation Method |
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| HU204946B (en) * | 1987-02-16 | 1992-03-30 | Novotrade R T | Method for increasing the effectiveness of "in vitro" vegetative propagation of potato |
| CN100487111C (en) * | 2005-11-09 | 2009-05-13 | 石河子大学 | A cultivation medium for test-tube seedling of detoxified potato and method for preparing the same |
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