CN107950530B - Application of the cinnamic acid in the preparation that preparation improves plant salt stress resistance - Google Patents
Application of the cinnamic acid in the preparation that preparation improves plant salt stress resistance Download PDFInfo
- Publication number
- CN107950530B CN107950530B CN201711264864.4A CN201711264864A CN107950530B CN 107950530 B CN107950530 B CN 107950530B CN 201711264864 A CN201711264864 A CN 201711264864A CN 107950530 B CN107950530 B CN 107950530B
- Authority
- CN
- China
- Prior art keywords
- cinnamic acid
- salt stress
- preparation
- plant
- stress resistance
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention provides application of the cinnamic acid in the preparation that preparation improves plant salt stress resistance, when the present invention is experimentally confirmed concentration≤20 μM for adding cinnamic acid in the medium, cinnamic acid will not influence the root growth of arabidopsis, but work as concentration>At 20 μM, with the increase of concentration, cinnamic acid also enhances the inhibiting effect of root long therewith.10 μM of optimum selecting of the present invention are subsequent experimental concentration as a result,.After adding 10 μM of cinnamic acids simultaneously in 150mM NaCl culture medium, salt stress inhibits to weaken to root growth, so that economic stress is eased.In simulation soil in salt stress experiment, after adding 10 μM of cinnamic acids in the plant nutrition liquid in the NaCl containing 100mM, the salt stress resistance of arabidopsis is significantly increased, the survival rate of plant is improved.The present invention is experimentally confirmed cinnamic acid and has potential application in terms of improving plant salt stress resistance.
Description
Technical field
The invention belongs to technical field of plant protection, and in particular to cinnamic acid improves the system of plant salt stress resistance in preparation
Application in agent.
Background technique
The soil salinization is to influence a key factor of agricultural production and ecological environment, due to industrial expansion and population
Be continuously increased, agriculture cultivated area is fewer and fewer, thus improve crop per unit area yield and resistance become the future of agriculture can hold
The important topic that supervention exhibition and environmental improvement are faced.Plant can show different characters, seriously when by environment stress
When will lead to death.Salt stress is an important environmental factor for influencing plant growth and development, therefore the Salt-resistance mechanism of plant needs
To further in-depth study.Seed is young after the sprouting and sprouting under salt stress as the most important propagation material of plant
The early growth situation of seedling reflects the saline-alkaline tolerance of plant to a certain extent, helps to inquire into it in different growing environments
Under the conditions of carry out culture application.
Cinnamic acid（cinnamic acid, CA）Also known as β-cinnamic acid, cinnamic acid, be from cassia or
The organic acid that styrax is isolated.It is degraded in plant by phenylalanine deamination and generates cinnamic acid.CA points are cis and trans, suitable
Formula be it is natural, it is trans- for synthesis.It is mainly used for flavors and fragrances, food additives, medical industry, beauty, pesticide, organic synthesis etc.
Aspect.
Summary of the invention
The case where for the above-mentioned prior art, the object of the present invention is to provide cinnamic acids to improve the plant salt side of body in preparation
Compel the application in the preparation of resistance, cinnamic acid purchase cost used in the present invention is low, aobvious to the remission effect of arabidopsis salt stress
It writes, and effect on environment is little.The present invention confirmed that external source adds low concentration cinnamic acid by experiment for the first time（Cinnamic
acid,CA）The salt stress resistance of arabidopsis can be improved.
For achieving the above object, the present invention is achieved by the following scheme：
The present invention provides application of the cinnamic acid in the preparation that preparation improves plant salt stress resistance.
Further：Addition concentration≤20 μM/L of cinnamic acid in the preparation.
Further：When the addition concentration of cinnamic acid is 10 μM/L in the preparation, Plants under Salt Stress root is significantly improved
System's growth.
Further：Concrete application method is：10 μM/L meat is added in the coercing cultivation base containing 150 mM NaCl
Cinnamic acid can improve arabidopsis Seedling root under salt stress and grow, alleviate the salt stress that seedling is subjected to.
Further：Concrete application method is：10 μM/L meat is added in the plant nutrition liquid containing 100 mM NaCl
Cinnamic acid significantly improves the salt stress resistance of arabidopsis, improves the survival rate of plant.
Further：The plant is arabidopsis.
Compared with prior art, it advantages of the present invention and has the technical effect that：The present invention is by experiment discovery of transplanting seedlings, 150
After adding 10 μM of CA simultaneously in mM NaCl coercing cultivation base, salt stress can be weakened to the inhibiting effect of root, can be improved quasi-
Southern mustard WT lines salt stress resistance.Cinnamic acid used in the present invention is cheap, environment-friendly, green ring
It protects, is widely used in life production.Influence based on salt stress to plant growth, it is thus found that applying the cinnamic acid of low concentration
The salt stress resistance of arabidopsis can be improved.In view of effect of the substance in salt stress, it is believed that the substance is to raising plant salt
Stress resistance has potential application.
Detailed description of the invention
Figure 1A is influence of the various concentration CA to arabidopsis root growth（1/2MS (-), 4 DEG C lamination three days, 22 DEG C of perpendicular trainings
Growth three days, is transferred to processing board, and 15 plants of each culture dish erects training growth 10 days）.
Figure 1B is the data statistics to root long.
Fig. 2A is different disposal condition：1/2MS (-),10 μM CA,150 mM NaCl,150 mM NaCl+10 μM
CA （1/2MS (-), 4 DEG C lamination three days, 22 DEG C are training growth three days perpendicular, are transferred to processing board, and 15 plants of each culture dish erects training life
It is 10 days long）.
Fig. 2 B is the data statistics to root long.
Fig. 3 A is different disposal condition：Control group（Contain only nutrient solution）,10 μM CA,100 mM NaCl,100 mM
NaCl+10 μM of CA simulates actual Soil salinity stress environment, grows one month.
Fig. 3 B is the data statistics to survival rate.
Specific embodiment
Technical solution of the present invention is further explained in following embodiment, according to above description and embodiment, originally
Field technical staff can determine essential characteristic of the invention, and without departing from the spirit and scope of the invention, can
To make various changes and modifications to the present invention, so that it is applicable in various uses and condition；Mentioned reagent is added to by the present invention
In model plant arabidopsis solid medium 1/2MS (-).Of the present invention is state of the art.
The present invention has purchased commercialization cinnamic acid first（Cinnamic acid）, it is equipped to close according to experimental concentration needs
The mother liquor of suitable concentration（100 mM/L）, it is added in 1/2MS (-) culture medium, is configured to the processing culture of different condition
Base.
Experimental material choose model plant arabidopsis wild type, it is all of above experiment be all made of it is of the same period it is in the same size,
The test seed of full seed, guarantees the consistency of experimental material.
Embodiment 1：The selection of concentration for the treatment of
One：The preparation of solid medium containing various concentration cinnamic acid
The present invention utilizes commercialization cinnamic acid（Cinnamic acid）It is configured to the mother liquor of suitable concentration（100 mM/L）,
It according to experimental concentration needs, is added in 1/2MS (-) culture medium, the processing culture medium of different condition is configured to, after progress
Continuous experiment of transplanting seedlings.
According to experimental considerations, contrived experiment journey is as follows：
1, the preparation of model plant arabidopsis solid medium
（1）Selection is used for the MS culture medium of Plant Tissue Breeding（Without agar and sucrose）, matched according to needed for experiment
It is made as 1/2MS (-) culture medium.Specific formula is as follows：Drug MS 2.37g/L is weighed, sucrose 15g/L is dissolved in ultrapure water
And 1000 ml are settled to, with 1M KOH solution and dilute hydrochloric acid tune pH to 5.80, as 1/2MS (-).
（2）It dispenses：Every 200 ml fluid nutrient medium is sub-packed in 250 ml triangular flasks, addition 1.7g agarose, and 121 DEG C
High temperature, high pressure sterilization 20 minutes.
（3）It after sterilizing, when culture medium is cooled to 50-60 DEG C, is added in super-clean bench into culture medium quantitative
CA mother liquor (100 mM/L), is configured to the CA solids treatment culture medium of concentration gradient, and respectively 0,10,20,30,40,50,60,
70,80,90,100 μM/L CA, is subsequently poured into glass dish, and every 200 ml dispenses 10 glass dishes.
（4）After culture medium solidification, it is spare to be sealed in sterile bag for label concentration.
2, seedling prepares
Plant Arabidopsis thaliana wild type in mode（Col-0）To handle material, in the same size, full seed of the same period is chosen
Test seed, seed uniline is layered on 1/2MS (-) solid medium by surface disinfection by chlorine 5h, 4 DEG C of dark laminations
Three days, 22 DEG C training growth three days perpendicular.
3, it transplants seedlings processing：
The consistent seedling of root long is selected to solids treatment culture medium, grows it in the same horizontal line, each culture dish
15 plants, 22 DEG C of long-day perpendicular training growth 10 days, as shown in Figure 1A.
Root long data and statistical result drawing are measured, as shown in Figure 1B.
Pass through interpretation of result of transplanting seedlings（Shown in Figure 1A and Figure 1B）, it is raw that addition concentration≤20 μM/L of CA does not influence plant
It is long, but>Inhibit plant strain growth when 20 μM/L.
This example has determined the addition concentration range of the reagent C A：≤ 20 μM/L.
Two：Optimal CA applies the determination of concentration
1, prepared by culture medium
（1）Selection is used for the MS culture medium of Plant Tissue Breeding（Without agar and sucrose）, matched according to needed for experiment
It is made as 1/2MS culture medium：1/2MS (-),150 mM NaCl.Specific formula is as follows：Weigh drug MS 2.37g/L, sucrose
15g/L is dissolved in ultrapure water and is settled to 1000 ml, with 1M KOH solution and dilute hydrochloric acid tune pH to 5.80, as 1/2MS
(-), basis is additional herein adds sodium chloride 58.44g/L, can be according to 150 mM NaCl culture mediums of required configuration.
（2）It dispenses：Every 200 ml is sub-packed in 250 ml triangular flasks, and 1.7g agarose, 121 DEG C of high temperature, high pressures are added
Sterilizing 20 minutes.
（3）It after sterilizing, when culture medium is cooled to 50-60 DEG C, is added in super-clean bench into culture medium quantitative
CA mother liquor is configured to different disposal culture medium, respectively 1/2MS (-), 10 μM of CA, 150 mM NaCl, 150 mM NaCl+
10 μM of CA, are subsequently poured into glass dish, and every 200 ml dispenses 10 glass dishes.
（4）After culture medium solidification, it is spare to be sealed in sterile bag for label concentration.
2, seedling prepares
Plant Arabidopsis thaliana wild type in mode（Col-0）To handle material, in the same size, full seed of the same period is chosen
Test seed, seed uniline is layered on 1/2MS (-) solid medium by surface disinfection by chlorine 5h, 4 DEG C of dark laminations
Three days, 22 DEG C training growth three days perpendicular.
3, it transplants seedlings processing：
The consistent seedling of root long is selected to various solids treatment culture mediums, grows it in the same horizontal line, Mei Gepei
15 plants of ware is supported, 22 DEG C of long-day erect training growth 10 days, as shown in Figure 2 A.
Root long data and statistical result drawing are measured, as shown in Figure 2 B.
Pass through interpretation of result of transplanting seedlings（As shown in Figure 2 A and 2 B）, when CA addition concentration is 10 μM/L, improve under salt stress
The effect of arabidopsis root growth is most obvious.
This example has determined the optimal addition concentration of the reagent C A：10 μM/L.
Embodiment 2
Embodiment and experiment effect now in conjunction with this experiment, further illustrate advantageous effects of the invention.
1, prepared by culture substrate
（1）Selection is used for the vermiculite culture substrate of plant growth, nutrient solution needed for the preparation plant growth according to needed for experiment
And 100 mM NaCl.
（2）It dispenses：Every 5L nutrient solution is sub-packed in glassware bottle.
（3）Processing grouping：Control group（Contain only nutrient solution）,10 μM CA,100 mM NaCl,100 mM NaCl + 10
Three groups of repetitions are arranged in μM CA, every kind of processing.
（4）Experiment process：Control group is used every time（Contain only nutrient solution）,10 μM CA,100 mM NaCl,100 mM
+ 10 μM of CA of NaCl submerge culture substrate, simulate actual salt stress environment.
2, material prepares
Plant Arabidopsis thaliana wild type in mode（Col-0）To handle material, in the same size, full seed of the same period is chosen
Test seed, seed is layered on vermiculite culture substrate with 3*3.
3, survival rate counts
Choosing experimental period is one month, observes the survival rate of arabidopsis under different disposal, as shown in Figure 3A.
Viable count and statistical result drawing are counted, as shown in Figure 3B.As shown in Figure 3A and Figure 3B, containing 100 mM
After adding 10 μM/L cinnamic acid in the plant nutrition liquid of NaCl, the salt stress resistance of arabidopsis is significantly improved, plant is improved
The survival rate of strain.
The above embodiments are merely illustrative of the technical solutions of the present invention, rather than is limited；Although referring to aforementioned reality
Applying example, invention is explained in detail, for those of ordinary skill in the art, still can be to aforementioned implementation
The technical solution that example is recorded is modified or equivalent replacement of some of the technical features；And these modifications or substitutions,
The spirit and scope for claimed technical solution of the invention that it does not separate the essence of the corresponding technical solution.
Claims (3)
1. application of the cinnamic acid in the preparation that preparation improves plant salt stress resistance, it is characterised in that：Cortex cinnamomi in the preparation
When the addition concentration of acid is 10 μM/L, Plants under Salt Stress root growth is significantly improved, the plant is arabidopsis.
2. application of the cinnamic acid according to claim 1 in the preparation that preparation improves plant salt stress resistance, feature
It is：Concrete application method is：10 μM/L cinnamic acid is added in the coercing cultivation base containing 150mM NaCl, can improve the salt side of body
Compel lower arabidopsis Seedling root growth, alleviates the salt stress that seedling is subjected to.
3. application of the cinnamic acid according to claim 1 in the preparation that preparation improves plant salt stress resistance, feature
It is：Concrete application method is：10 μM/L cinnamic acid is added in the plant nutrition liquid containing 100mM NaCl, is significantly improved quasi-
The salt stress resistance of southern mustard, improves the survival rate of plant.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711264864.4A CN107950530B (en) | 2017-12-05 | 2017-12-05 | Application of the cinnamic acid in the preparation that preparation improves plant salt stress resistance |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711264864.4A CN107950530B (en) | 2017-12-05 | 2017-12-05 | Application of the cinnamic acid in the preparation that preparation improves plant salt stress resistance |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107950530A CN107950530A (en) | 2018-04-24 |
| CN107950530B true CN107950530B (en) | 2018-11-23 |
Family
ID=61963191
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711264864.4A Expired - Fee Related CN107950530B (en) | 2017-12-05 | 2017-12-05 | Application of the cinnamic acid in the preparation that preparation improves plant salt stress resistance |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107950530B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115669665B (en) * | 2022-10-13 | 2023-12-12 | 中国科学院东北地理与农业生态研究所 | Application of ferulic acid in promoting root growth and seedling development of Arabidopsis thaliana |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104195150A (en) * | 2014-09-25 | 2014-12-10 | 山东大学 | Application of arabidopsis glycosyl transferase gene UGT79B2 in improving salt resistance and drought resistance of plants |
-
2017
- 2017-12-05 CN CN201711264864.4A patent/CN107950530B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN107950530A (en) | 2018-04-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Mahmoud et al. | Silicon nanoparticles mitigate oxidative stress of in vitro-derived banana (Musa acuminata ‘Grand Nain’) under simulated water deficit or salinity stress | |
| Ammirato | The regulation of somatic embryo development in plant cell cultures: suspension culture techniques and hormone requirements | |
| Bahmani et al. | Effects of salinity on in vitro shoot proliferation and rooting of apple rootstock MM. 106 | |
| CN107135952A (en) | A kind of method for promoting tissue culture seedlings of bananas to take root with strengthening root | |
| CN103540607B (en) | The new opplication of Arabidopis thaliana At4g09820 gene in plant anti-salt | |
| CN104938337B (en) | Dendrobium officinale rapid propagation and seedling culture medium series and tissue culture method | |
| CN107950530B (en) | Application of the cinnamic acid in the preparation that preparation improves plant salt stress resistance | |
| CN102577690A (en) | Method for promoting eel grass seeds to germinate and grow | |
| CN103416310B (en) | Culture medium used for roxburgh anoectochilus terminal bud organic tissue culture | |
| CN102119658A (en) | Method for culturing aseptic seedlings of plants | |
| CN108611277A (en) | A kind of selenium-rich gill fungus bacterium strain, selenium-rich gill fungus bacterium and its breeding method | |
| Nayekova et al. | Impact of diatomite priming of seeds of hordeum vulgaris in salinity. | |
| CN105993911A (en) | Hybridization breeding method for high-yield maize | |
| CN102187773A (en) | Cultivation method for improving salt tolerance of eggplant by applying external source NO to root | |
| CN105543251A (en) | Disease-resistance-related gene AtADH1 of arabidopsis thaliana as well as preparation method and disease-resistant application of disease-resistance-related gene AtADH1 | |
| Abdelgalil et al. | Effect of different plant bio-stimulants in improving cucumber growth under soilless culture | |
| CN100411508C (en) | Cultivation method for highly effective plant tissue differentiation and regeneration | |
| Rekha et al. | A protocol for rapid screening of rice lines for seedling stage salinity tolerance | |
| Kumar et al. | In Vitro Propagation Journey of Ornamental Gladiolus (Gladiolus Species): a systematic review analysis based on more than 50 years Research | |
| Rihan | Plant tissue culture and artificial seed production techniques for cauliflower and their use to study molecular analysis of abiotic stress tolerance | |
| Shah et al. | Cost effective micropropagation technology for potatoes | |
| RU2577106C2 (en) | Method for increasing resistance of sugar beet crops to phytotoxioc impact of herbicides | |
| CN111254081B (en) | Large-scale seed preservation and expansion culture method for pure benthic diatom | |
| CN101381732A (en) | Use of gene OsGS1;2 in improving resistance of rice to herbicides Basta | |
| Ali | Behavior of Wheat Cultivars in Hydroponic Culture under Saline Conditions |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20181123 Termination date: 20211205 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |