CN108611277A - A kind of selenium-rich gill fungus bacterium strain, selenium-rich gill fungus bacterium and its breeding method - Google Patents
A kind of selenium-rich gill fungus bacterium strain, selenium-rich gill fungus bacterium and its breeding method Download PDFInfo
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Abstract
The present invention provides a kind of breeding method of selenium-rich gill fungus bacterium, includes the following steps：Step 1: acclimation and screening, first class inoculum is obtained, going out the strong gill fungus bacterium strain of selenium rich ability by acclimation and screening be used as first class inoculum, and record cultivates the concentration of sodium selenite of first class inoculum as optimal concentration of sodium selenite, and preservation first class inoculum；Step 2: preparing culture material containing selenium：Culture material is made, sodium selenite solution is added in culture material according to optimal concentration of sodium selenite in step 1 and obtains culture material containing selenium；Step 3: being inoculated into first class inoculum in culture material containing selenium, suitable temperature, humidity and acid-base value are kept, first class inoculum is seeded in culture material containing selenium, grows selenium-rich gill fungus bacterium.The present invention changes conventional selenium-rich gill fungus bacterium type of rearing, can improve the organic selenium content in gill fungus bacterium energetically, improves production efficiency.
Description
Technical field
The present invention relates to modern agriculture technical field of cultivation more particularly to a kind of selenium-rich gill fungus bacterium strain, selenium-rich gill fungus bacterium and its
Breeding method.
Background technology
Selenium (Se) is indispensable important member in vital movement although content in human body only 0.1-0.2ppm
Element.Selenium, which has, to be improved immunity of organisms, promotes rehabilitation, anticancer and anti-aging and other effects.Chinese Soclety of Nutrition is recommended " every
Regulation in the dietary guidelines of nutrient supply and China in day diet ", the day required intake adult and juvenile micro- for 50 of selenium
Gram, children are 20 (1-3 Suis) to 40 (4-6 Sui) micrograms, this is the lower limit of necessary amounts.According to investigation show, at present the whole nation at
Selenium mean intake only 26.6 micrograms/day of people the 1/2 and preferably horizontal 1/10 of necessary amounts lower limit is almost only reached, therefore
Vast majority of people is required for increasing the intake of selenium as early as possible, and should lifelong selenium-supply.
China once used inorganic selenium salt sodium selenite prevention Keshan disease and Kaschin-Beck disease, obtained remarkable result.But use nothing
Machine selenium salt selenium-supply, absorbs and use aspects are all undesirable, and intake does not work less, slightly excessive just to have toxicity.Therefore
In recent years all tend to use Organic Selenium.In addition to the pesticide herd product in high selenium area, main fungus and algae using rich in selenium element.Institute
Selenium-rich gill fungus bacterium is called, refers to just food bacterium or the medicine bacterium that selenium content is much higher than wholefood.Selenium-rich gill fungus bacterium can both make tonic food
Product, and medicament can be made, remaining mushroom bran or splendid Se-enriched feedstuff and fertilizer, production cost is not high, and measures supply
Operability it is preferable, have good benefit.
Current selenium-rich gill fungus bacterium inoculation method generally uses two ways：1, the training of gill fungus bacterium strain directly is added in inorganic selenium
It supports in base, allows in gill fungus bacterium mycelia growth course the selenium element absorbed in culture medium, to grow up to the selenium-rich gill fungus bacterium containing selenium element；
2, Selenium-rich nutrient solution mode is sprayed to bacteria stick：As CN1823570A discloses a kind of cultural method of selenium-enriched edible mushroom, selenium-rich food
With bacterium raw material by the per distribution ratio by weight such as corncob, straw powder, rice bran or wheat bran, gypsum, magnesium sulfate, zinc sulfate, selenium salt, water
It is made；Pack is sealed, and sterilizing accesses edible bacterium, then ferments, fermentation time 20-40 days, mycelia covers with entire charge level, i.e.,
Bacteria stick is made, then sprays Selenium-rich nutrient solution.
The selenium-rich gill fungus bacterium organic selenium content that both the above mode obtains is about 0.1ppm or so, and organic selenium content is too low cannot
Meet edible and medicinal demand, efficiency is low, and the selenium overwhelming majority that above two mode obtains is inorganic selenium, easy tos produce poison
Side effect is harmful to health, therefore urgently develops a kind of new selenium-rich gill fungus bacterium inoculation method, can energetically improve in gill fungus bacterium
Organic selenium content, improve production efficiency, avoid toxicity, ensure health.Start with from selenium-rich gill fungus bacterium is cultivated, Jin Ersheng
The food and drug for producing selenium-supply function, are a feasible evolutionary paths.With preferable social benefit, ecological benefits and economy
Benefit.
Invention content
In view of the drawbacks of the prior art, the present invention is intended to provide a kind of new selenium-rich gill fungus bacterium inoculation method, can carry energetically
Se content and organic selenium content in high gill fungus bacterium reduce inorganic selenium, improve and cultivate efficiency, avoid toxicity, ensure that human body is strong
Health.
Technical scheme of the present invention：
A kind of breeding method of selenium-rich gill fungus bacterium, includes the following steps：
Step 1: acclimation and screening, obtains first class inoculum：Prepare candidate gill fungus bacterium strain, makes first class inoculum culture medium, it is quantitative
It dispenses first class inoculum culture medium and adds the sodium selenite solution of various concentration wherein respectively, obtain being trained containing selenium for various concentration
Base is supported, candidate gill fungus bacterium strain is seeded to and is cultivated containing in seleno culture medium for various concentration, suitable temperature, humidity and soda acid are kept
Degree obtains the strong candidate gill fungus bacterium strain of selenium rich ability by acclimation and screening and is used as first class inoculum, the Asia of record culture first class inoculum
Selenic acid na concn is as optimal concentration of sodium selenite, and preservation first class inoculum；
Step 2: preparing culture material containing selenium：Culture material is made, according to the optimal concentration of sodium selenite in step 1 by sub- selenium
Acid sodium solution, which is added in culture material, obtains culture material containing selenium；
Step 3: being inoculated into first class inoculum in culture material containing selenium, suitable temperature, humidity and acid-base value are kept, is obtained
Selenium-rich gill fungus bacterium.
Preferably, the step 1 includes the following steps：
(1) 2 or more the different gill fungus bacterium strains that selection resistance is strong, growth is fast, yield is high, as candidate gill fungus bacterium strain；
(2) first class inoculum culture medium is made；
(3) quantitative separating first class inoculum culture medium and difference add the sodium selenite solution of various concentration wherein, obtain
Various concentration contains seleno culture medium；
(4) by the candidate gill fungus bacterium strain in step (1) be inoculated into respectively step (3) various concentration containing in seleno culture medium,
20-28 DEG C of temperature, humidity 60-70%, PH6-7 is kept to cultivate 7-15 days；
(5) in observation of steps (4) candidate gill fungus bacterium strain in various concentration containing the growing state in seleno culture medium, select bacterium
The vigorous healthy and strong, speed of growth of silk growth it is fast be used as first class inoculum, the concentration of sodium selenite of record culture first class inoculum is as most
Excellent concentration of sodium selenite, and preservation first class inoculum.
Preferably, the sodium selenite solution of the addition various concentration in the step (3) refers in first class inoculum culture medium
The middle selenous acid for adding 30ppm, 60ppm, 90ppm, 120ppm, 150ppm, 180ppm, 210ppm, 240ppm, 270ppm respectively
Sodium solution.
Preferably, increase step 4 after the step 3：Before being reused to the first class inoculum of preservation in step 1, detection
The selenium rich ability of the first class inoculum of preservation, select mycelia grow the vigorous healthy and strong, speed of growth it is fast be used as first class inoculum again, weigh
Multiple step 2 and step 3.
Preferably, the selenium rich ability of the detection preservation first class inoculum refers to that preservation first class inoculum is seeded to first class inoculum
The selenium rich ability that preservation first class inoculum is detected in culture medium, select mycelia grow the vigorous healthy and strong, speed of growth soon again as
First class inoculum.
Preferably, increase step 5 after the step 4：The selenium rich ability for detecting the first class inoculum of preservation, to selenium rich ability
The first class inoculum of difference is purificated and rejuvenated.
Preferably, the first class inoculum culture medium in the step 1 is PDA culture medium, is wrapped in every 1000mlPDA culture mediums
Include the raw material components of following weight ratio：Peeled potatoes 200g, glucose 20g, potassium dihydrogen phosphate 1.5g, dipotassium hydrogen phosphate 1.5g,
Magnesium sulfate 3g, vitamin B11 0mg, agar 20g, remaining is water.
Preferably, the culture material in the step 3 includes the raw material components of following weight ratio：Cotton seed hulls 75%, corncob
18%, wheat bran 10%, lime 1.5%, gypsum 1%, magnesium sulfate 0.2%, water 65%.
A kind of selenium-rich gill fungus bacterium strain, according to first class inoculum made from a kind of above-mentioned breeding method of selenium-rich gill fungus bacterium.
A kind of selenium-rich gill fungus bacterium, using a kind of above-mentioned selenium-rich gill fungus bacterium that the breeding method of selenium-rich gill fungus bacterium obtains.
Compared with prior art, the invention has the advantages that：The present invention is strong using first acclimation and screening selenium rich ability
First class inoculum, then expand cultivation obtain selenium-rich gill fungus bacterium breeding method, by selenium-rich gill fungus bacterium organic selenium content from 0.1ppm promoted
To 100-500ppm, yield is about 1000-5000 times of existing product.The selenium-rich gill fungus bacterium Organic Selenium obtained in this way contains
Amount accounts for 90% or more of total selenium content, and amino acid content also significantly improves therewith.The present invention can improve having in gill fungus bacterium energetically
Machine Se content reduces inorganic selenium, improves production efficiency, avoids toxicity, ensures health.
Description of the drawings
Fig. 1 is the process flow chart of the present invention；
Fig. 2 is the process flow chart of step 1 of the present invention.
Specific implementation mode
Shown in referring to Figures 1 and 2, technical solution to facilitate the understanding of the present invention, of the invention is typical but non-limiting
Embodiment it is as follows：
Embodiment one
First class inoculum culture medium is made in following manner：
(1) potato decortication removes bud eye, weighs 200g, is cut into 1-2 grams of hobboing cutter block, is put into pot, and 1000ml water is added,
10min is boiled after boiling again.With 4-6 layers of filtered through gauze waste, filtrate is refunded in pot, and agar 20g is added, is used when being heated with slow boiling
Glass bar stirs, until agar be added after dissolving glucose 20g, potassium dihydrogen phosphate 1.5g, dipotassium hydrogen phosphate 1.5g, magnesium sulfate 3g,
Vitamin B11 0mg.Add water to complement to 1000ml, pour into graduated cylinder while hot, obtains fluid nutrient medium.
(2) funnel being placed on the clip of iron stand, mouth connects emulsion tube under funnel, and a spring clip is pressed from both sides on pipe, as
Control switchs, and tip glass tube is connect under pipe.Fluid nutrient medium is noted in funnel while hot, tip glass tube is inserted into 8-180cm
Test tube in, open spring clip allows fluid nutrient medium to flow into test tube, liquid, each test tube can not be speckled at the 3cm in test tube mouth
Contained liquid culture medium 8ml, test tube mouth add tampon.Tampon keeps 1/3 outside pipe, 2/3 in pipe chock off, and 7 test tubes are stranded into one
, the one end for being plugged with tampon is wrapped with waterproof paper, is tightened with cotton rope, test tube is stood up in iron wire frame, in case sterilizing.
(3) 3000-3500ml water is added in portable pressure cooker, small iron wire frame is disposed within, on small iron wire frame
Newspaper is covered, is covered, tightens and is heated to 125 DEG C.Boiling water final vacuum 10-20min, turns off deflation valve, make pressure by
Gradually rise.When pressure reaches 0.12MPa, start timing, after maintaining constant pressure 30min, closes heat source, pressure is allowed slowly to drop to
Zero.
(4) open pot cover, when temperature be down to there is no droplet on 60 DEG C and test tube wall when, one end of test tube mouth is rested on into batten
On, the other end is put forms certain gradient on the table, by inclined-plane adjustment at the 3/5 of test tube length, waits for that culture medium condenses,
First class inoculum culture medium, which is prepared, at this time completes, and moves in insulating box and is cultivated 3-7 days at 25 DEG C, refrigerator 4 is put into after confirmation is pollution-free
It DEG C saves backup.
Embodiment two
Mushroom with abundant selenium is prepared in accordance with the following steps：
Step 1: acclimation and screening, obtains first class inoculum：
(1) 2 flat mushroom strains that selection resistance is strong, growth is fast, yield is high, as candidate flat mushroom strain.
(2) first class inoculum culture medium is made；First class inoculum culture medium is PDA culture medium, is wrapped in every 1000mlPDA culture mediums
Include the raw material components of following weight ratio：Peeled potatoes 200g, glucose 20g, potassium dihydrogen phosphate 1.5g, dipotassium hydrogen phosphate 1.5g,
Magnesium sulfate 3g, vitamin B11 0mg, agar 20g, remaining is water.
(3) prepare 18 test tubes, be divided into two groups i.e. first group and second group by 9 every group of test tubes, respectively first group, the
First class inoculum culture medium 8ml is dispensed in two groups of test tubes, and add respectively in first group of every test tube 30ppm, 60ppm,
The sodium selenite solution of 90ppm, 120ppm, 150ppm, 180ppm, 210ppm, 240ppm, 270ppm, in second group of every examination
The sub- selenium of 30ppm, 60ppm, 90ppm, 120ppm, 150ppm, 180ppm, 210ppm, 240ppm, 270ppm are added in pipe respectively
Acid sodium solution, obtain two groups of 9 kinds of various concentrations contains seleno culture medium.
(4) contain selenium by what 2 kinds in step (1) candidate flat mushroom strains were inoculated into (3) two groups of 9 kinds of various concentrations of step respectively
In culture medium：I.e. by the first candidate flat mushroom strain be seeded to respectively first group containing 30ppm, 60ppm, 90ppm, 120ppm,
The sodium selenite solution of 150ppm, 180ppm, 210ppm, 240ppm, 270ppm contain in seleno culture medium, then candidate by second
Flat mushroom strain be seeded to respectively second group containing 30ppm, 60ppm, 90ppm, 120ppm, 150ppm, 180ppm, 210ppm,
The sodium selenite solution of 240ppm, 270ppm contain in seleno culture medium, 20-28 DEG C of temperature of holding, humidity 60-70%, PH6-7,
Culture 7 days.
(5) in observation of steps (4) candidate flat mushroom strain in various concentration containing the growing state in seleno culture medium, select bacterium
The vigorous healthy and strong, speed of growth of silk growth it is fast be used as first class inoculum, the speed of growth described here refers to that 7-10 days mycelia are long soon
Full inclined-plane, for example, it is most excellent containing the first candidate flat mushroom strain growing state in seleno culture medium in first group of 30ppm, then it selects
The first candidate flat mushroom strain is as first class inoculum, and the concentration of sodium selenite of record culture first class inoculum is as optimal sodium selenite
Concentration, such as 30ppm, and preservation first class inoculum.
Step 2: preparing culture material containing selenium：Culture material is made, according to optimal concentration of sodium selenite in step 1, for example is walked
Suddenly the 30ppm of (5) record, 30ppm sodium selenite solutions are added in culture material and are obtained containing selenium (30ppm) culture material；Culture material
Include the raw material components of following weight ratio：Cotton seed hulls 75%, corncob 18%, wheat bran 10%, lime 1.5%, gypsum 1%, sulphur
Sour magnesium 0.2%, water 65%.
Step 3: being inoculated into first class inoculum (such as the first candidate flat mushroom strain) containing in selenium (such as 30ppm) culture material, protect
20-28 DEG C of temperature is held, humidity 60-70%, PH6-7 grow mushroom with abundant selenium.
Step 4：Before being reused to the first class inoculum of preservation in step 1, the selenium-rich energy of the first class inoculum of preservation is detected
Power, select mycelia grow the vigorous healthy and strong, speed of growth it is fast be used as first class inoculum again, repeatedly step 2 and step 3.
The selenium rich ability of the detection preservation first class inoculum refers to that preservation first class inoculum is seeded to first class inoculum culture medium
The selenium rich ability of middle detection preservation first class inoculum, select mycelia grow the vigorous healthy and strong, speed of growth soon again as level-one bacterium
Kind.
Step 5：The selenium rich ability for detecting the first class inoculum of preservation to the first class inoculum of selenium rich ability difference purify multiple
It is strong.
Purification and rejuvenation include the following steps：(1) first class inoculum culture medium is made；First class inoculum culture medium is PDA culture medium,
Include the raw material components of following weight ratio per 1000mlPDA culture mediums：Peeled potatoes 200g, glucose 20g, potassium dihydrogen phosphate
1.5g, dipotassium hydrogen phosphate 1.5g, magnesium sulfate 3g, vitamin B11 0mg, agar 20g, remaining is water.(2) selection selenium rich ability is poor
First class inoculum, be seeded in first class inoculum culture medium.(3) 20-28 DEG C of temperature, humidity 60-70%, PH6-7 is kept to cultivate 7-
15 days, select without miscellaneous bacteria, mycelia grow vigorous healthy and strong, strain that the speed of growth is fast to get.
The mushroom with abundant selenium prepared in the above manner, physical and chemical index can reach following table requirement：
| Project | Index | The method of inspection |
| Selenium/(mg/kg) | 100.0-500.0 | GB 5009.93 |
| Organic Selenium account for total selenium mass percent/(w/%) >= | 95.0 | Test method |
| Protein/(g/100g) >= | 16.0 | GB 5009.5 |
| Dietary fiber/(g/100g) >= | 18.0 | GB 5009.88 |
| Moisture/(g/100g) >= | 11.0 | GB 5009.3 |
| Ash content/(g/100g)≤ | 8.5 | GB 5009.4 |
| Lead (in terms of Pb)/mg/kg≤ | 0.8 | GB 5009.12 |
| Cadmium (in terms of Cd)/mg/kg≤ | 0.5 | GB 5009.15 |
| Total mercury (in terms of Hg)/mg/kg≤ | 0.1 | GB 5009.17 |
| Total arsenic (in terms of As)/mg/kg≤ | 0.5 | GB 5009.11 |
| Pesticide residue | GB 2763 |
Wherein, test method refers to that inorganic selenium is detached with Organic Selenium through being extracted under the conditions of 6mol/L hydrochloric acid water-baths in sample,
With the content of AFS DETERMINATION inorganic selenium.Organic selenium content is the difference of total selenium and inorganic selenium.
Embodiment three
Mushroom with abundant selenium is prepared in accordance with the following steps：
Step 1: acclimation and screening, obtains first class inoculum：
(1) 5 flat mushroom strains that selection resistance is strong, growth is fast, yield is high, as candidate flat mushroom strain.
(2) first class inoculum culture medium is made；First class inoculum culture medium is PDA culture medium, is wrapped in every 1000mlPDA culture mediums
Include the raw material components of following weight ratio：Peeled potatoes 200g, glucose 20g, potassium dihydrogen phosphate 1.5g, dipotassium hydrogen phosphate 1.5g,
Magnesium sulfate 3g, vitamin B11 0mg, agar 20g, remaining is water.
(3) prepare 45 test tubes, by 9 every group of test tubes be divided into five groups i.e. first group, second group, third group, the 4th group and
5th group, first class inoculum culture medium 8ml is dispensed in first group, second group, third group, the 4th group and the 5th group of test tube respectively,
And added respectively in first group of every test tube 30ppm, 60ppm, 90ppm, 120ppm, 150ppm, 180ppm, 210ppm,
The sodium selenite solution of 240ppm, 270ppm, added respectively in second group of every test tube 30ppm, 60ppm, 90ppm,
The sodium selenite solution of 120ppm, 150ppm, 180ppm, 210ppm, 240ppm, 270ppm divide in every test tube of third group
Not Tian Jia 30ppm, 60ppm, 90ppm, 120ppm, 150ppm, 180ppm, 210ppm, 240ppm, 270ppm sodium selenite it is molten
Liquid, added respectively in the 4th group of every test tube 30ppm, 60ppm, 90ppm, 120ppm, 150ppm, 180ppm, 210ppm,
The sodium selenite solution of 240ppm, 270ppm, added respectively in the 5th group of every test tube 30ppm, 60ppm, 90ppm,
The sodium selenite solution of 120ppm, 150ppm, 180ppm, 210ppm, 240ppm, 270ppm obtain five groups of 9 kinds of various concentrations
Containing seleno culture medium.
(4) contain selenium by what 5 kinds in step (1) candidate flat mushroom strains were inoculated into (3) five groups of 9 kinds of various concentrations of step respectively
In culture medium：I.e. by the first candidate flat mushroom strain be seeded to respectively first group containing 30ppm, 60ppm, 90ppm, 120ppm,
The sodium selenite solution of 150ppm, 180ppm, 210ppm, 240ppm, 270ppm contain in seleno culture medium, candidate flat by second
Mushroom strains be seeded to respectively second group containing 30ppm, 60ppm, 90ppm, 120ppm, 150ppm, 180ppm, 210ppm, 240ppm,
The sodium selenite solution of 270ppm contains in seleno culture medium, the third candidate's flat mushroom strain is seeded to third group respectively to be contained
The sodium selenite solution of 30ppm, 60ppm, 90ppm, 120ppm, 150ppm, 180ppm, 210ppm, 240ppm, 270ppm contain
In seleno culture medium, by the 4th kind of candidate flat mushroom strain be seeded to respectively the 4th group containing 30ppm, 60ppm, 90ppm, 120ppm,
Containing in seleno culture medium for the sodium selenite solution of 150ppm, 180ppm, 210ppm, 240ppm, 270ppm, the 5th kind of candidate is put down
Mushroom strains be seeded to respectively the 5th group containing 30ppm, 60ppm, 90ppm, 120ppm, 150ppm, 180ppm, 210ppm, 240ppm,
The sodium selenite solution of 270ppm contains in seleno culture medium, and 20-28 DEG C of temperature, humidity 60-70%, PH6-7 is kept to cultivate 9 days.
(5) in observation of steps (4) candidate flat mushroom strain in various concentration containing the growing state in seleno culture medium, select bacterium
The vigorous healthy and strong, speed of growth of silk growth it is fast be used as first class inoculum, the speed of growth described here refers to that 7-10 days mycelia are long soon
Full inclined-plane, for example, it is most excellent containing the third candidate flat mushroom strain growing state in seleno culture medium in third group 90ppm, then it selects
The third candidate flat mushroom strain is as first class inoculum, and the concentration of sodium selenite of record culture first class inoculum is as optimal sodium selenite
Concentration, such as 90ppm, and preservation first class inoculum.
Step 2: preparing culture material containing selenium：Culture material is made, according to optimal concentration of sodium selenite in step 1, for example is walked
Suddenly the 90ppm of (5) record, 90ppm sodium selenite solutions are added in culture material and are obtained containing selenium (90ppm) culture material；Culture material
Include the raw material components of following weight ratio：Cotton seed hulls 75%, corncob 18%, wheat bran 10%, lime 1.5%, gypsum 1%, sulphur
Sour magnesium 0.2%, water 65%.
Step 3: being inoculated into first class inoculum (such as the third candidate flat mushroom strain) containing in selenium (such as 90ppm) culture material, protect
20-28 DEG C of temperature is held, humidity 60-70%, PH6-7 grow mushroom with abundant selenium.
Step 4：Before being reused to the first class inoculum of preservation in step 1, the selenium-rich energy of the first class inoculum of preservation is detected
Power, select mycelia grow the vigorous healthy and strong, speed of growth it is fast be used as first class inoculum again, repeatedly step 2 and step 3.
The selenium rich ability of the detection preservation first class inoculum refers to that preservation first class inoculum is seeded to first class inoculum culture medium
The selenium rich ability of middle detection preservation first class inoculum, select mycelia grow the vigorous healthy and strong, speed of growth soon again as level-one bacterium
Kind.
Step 5：The selenium rich ability for detecting the first class inoculum of preservation to the first class inoculum of selenium rich ability difference purify multiple
It is strong.
Purification and rejuvenation include the following steps：(1) first class inoculum culture medium is made；First class inoculum culture medium is PDA culture medium,
Include the raw material components of following weight ratio per 1000mlPDA culture mediums：Peeled potatoes 200g, glucose 20g, potassium dihydrogen phosphate
1.5g, dipotassium hydrogen phosphate 1.5g, magnesium sulfate 3g, vitamin B11 0mg, agar 20g, remaining is water.(2) selection selenium rich ability is poor
First class inoculum, be seeded in first class inoculum culture medium.(3) 20-28 DEG C of temperature, humidity 60-70%, PH6-7 is kept to cultivate 7-
15 days, select without miscellaneous bacteria, mycelia grow vigorous healthy and strong, strain that the speed of growth is fast to get.
The mushroom with abundant selenium prepared in the above manner, physical and chemical index can reach following table requirement：
Wherein, test method refers to that inorganic selenium is detached with Organic Selenium through being extracted under the conditions of 6mol/L hydrochloric acid water-baths in sample,
With the content of AFS DETERMINATION inorganic selenium.Organic selenium content is the difference of total selenium and inorganic selenium.
Example IV
Selenium-rich phoenix-tail mushroom is prepared in accordance with the following steps：
Step 1: acclimation and screening, obtains first class inoculum：
(1) 3 phoenix tail mushroom strains that selection resistance is strong, growth is fast, yield is high, as candidate phoenix tail mushroom strains.
(2) first class inoculum culture medium is made；First class inoculum culture medium is PDA culture medium, is wrapped in every 1000mlPDA culture mediums
Include the raw material components of following weight ratio：Peeled potatoes 200g, glucose 20g, potassium dihydrogen phosphate 1.5g, dipotassium hydrogen phosphate 1.5g,
Magnesium sulfate 3g, vitamin B11 0mg, agar 20g, remaining is water.
(3) prepare 27 test tubes, be divided into three groups i.e. first group, second group and third group by 9 every group of test tubes, respectively the
One group, second group and the interior packing first class inoculum culture medium 8ml of third group test tube, and added respectively in first group of every test tube
The sodium selenite solution of 30ppm, 60ppm, 90ppm, 120ppm, 150ppm, 180ppm, 210ppm, 240ppm, 270ppm,
Added respectively in second group of every test tube 30ppm, 60ppm, 90ppm, 120ppm, 150ppm, 180ppm, 210ppm, 240ppm,
The sodium selenite solution of 270ppm, added respectively in every test tube of third group 30ppm, 60ppm, 90ppm, 120ppm,
The sodium selenite solution of 150ppm, 180ppm, 210ppm, 240ppm, 270ppm, the selenium that contains for obtaining three groups of 9 kinds of various concentrations are trained
Support base.
(4) 3 kinds of candidate phoenix tail mushroom strains in step (1) are inoculated into containing for (3) three groups of 9 kinds of various concentrations of step respectively
In seleno culture medium：I.e. by the first candidate phoenix tail mushroom strains be seeded to respectively first group containing 30ppm, 60ppm, 90ppm,
The sodium selenite solution of 120ppm, 150ppm, 180ppm, 210ppm, 240ppm, 270ppm contain in seleno culture medium, by second
Kind of candidate phoenix tail mushroom strains be seeded to respectively second group containing 30ppm, 60ppm, 90ppm, 120ppm, 150ppm, 180ppm,
The sodium selenite solution of 210ppm, 240ppm, 270ppm contain in seleno culture medium, the third candidate's phoenix tail mushroom strains is connect respectively
It plants to sub- selenium of the third group containing 30ppm, 60ppm, 90ppm, 120ppm, 150ppm, 180ppm, 210ppm, 240ppm, 270ppm
Acid sodium solution contains in seleno culture medium, and 20-28 DEG C of temperature, humidity 60-70%, PH6-7 is kept to cultivate 10 days.
(5) in observation of steps (4) candidate phoenix tail mushroom strains in various concentration containing the growing state in seleno culture medium, selection
Mycelia grows vigorous conduct first class inoculum healthy and strong, the speed of growth is fast, and the speed of growth described here refers to 7-10 days mycelia soon
Inclined-plane is covered with, such as most excellent containing second of candidate phoenix-tail mushroom growth situation in seleno culture medium in second group of 120ppm,
Then select second of candidate phoenix tail mushroom strains as first class inoculum, the concentration of sodium selenite of record culture first class inoculum is as optimal
Concentration of sodium selenite, such as 120ppm, and preservation first class inoculum.
Step 2: preparing culture material containing selenium：Culture material is made, according to optimal concentration of sodium selenite in step 1, for example is walked
Suddenly the 120ppm of (5) record, 120ppm sodium selenite solutions are added in culture material and are obtained containing selenium (120ppm) culture material；Cultivation
Material includes the raw material components of following weight ratio：Cotton seed hulls 75%, corncob 18%, wheat bran 10%, lime 1.5%, gypsum 1%,
Magnesium sulfate 0.2%, water 65%.
Step 3: being inoculated into first class inoculum (such as second candidate phoenix tail mushroom strains) containing selenium (such as 120ppm) culture material
In, 20-28 DEG C of temperature is kept, humidity 60-70%, PH6-7 grow selenium-rich phoenix-tail mushroom.
Step 4：Before being reused to the first class inoculum of preservation in step 1, the selenium-rich energy of the first class inoculum of preservation is detected
Power, select mycelia grow the vigorous healthy and strong, speed of growth it is fast be used as first class inoculum again, repeatedly step 2 and step 3.
The selenium rich ability of the detection preservation first class inoculum refers to that preservation first class inoculum is seeded to first class inoculum culture medium
The selenium rich ability of middle detection preservation first class inoculum, select mycelia grow the vigorous healthy and strong, speed of growth soon again as level-one bacterium
Kind.
Step 5：The selenium rich ability for detecting the first class inoculum of preservation to the first class inoculum of selenium rich ability difference purify multiple
It is strong.
Purification and rejuvenation include the following steps：(1) first class inoculum culture medium is made；First class inoculum culture medium is PDA culture medium,
Include the raw material components of following weight ratio per 1000mlPDA culture mediums：Peeled potatoes 200g, glucose 20g, potassium dihydrogen phosphate
1.5g, dipotassium hydrogen phosphate 1.5g, magnesium sulfate 3g, vitamin B11 0mg, agar 20g, remaining is water.(2) selection selenium rich ability is poor
First class inoculum, be seeded in first class inoculum culture medium.(3) 20-28 DEG C of temperature, humidity 60-70%, PH6-7 is kept to cultivate 7-
15 days, select without miscellaneous bacteria, mycelia grow vigorous healthy and strong, strain that the speed of growth is fast to get.
The selenium-rich phoenix-tail mushroom prepared in the above manner, physical and chemical index can reach following table requirement：
Wherein, test method refers to that inorganic selenium is detached with Organic Selenium through being extracted under the conditions of 6mol/L hydrochloric acid water-baths in sample,
With the content of AFS DETERMINATION inorganic selenium.Organic selenium content is the difference of total selenium and inorganic selenium.
Embodiment five
Se-rich xianggu is prepared in accordance with the following steps：
Step 1: acclimation and screening, obtains first class inoculum：
(1) 5 mushroom strains that selection resistance is strong, growth is fast, yield is high, as candidate mushroom strain.
(2) first class inoculum culture medium is made；First class inoculum culture medium is PDA culture medium, is wrapped in every 1000mlPDA culture mediums
Include the raw material components of following weight ratio：Peeled potatoes 200g, glucose 20g, potassium dihydrogen phosphate 1.5g, dipotassium hydrogen phosphate 1.5g,
Magnesium sulfate 3g, vitamin B11 0mg, agar 20g, remaining is water.
(3) prepare 45 test tubes, by 9 every group of test tubes be divided into five groups i.e. first group, second group, third group, the 4th group and
5th group, first class inoculum culture medium 8ml is dispensed in first group, second group, third group, the 4th group and the 5th group of test tube respectively,
And added respectively in first group of every test tube 30ppm, 60ppm, 90ppm, 120ppm, 150ppm, 180ppm, 210ppm,
The sodium selenite solution of 240ppm, 270ppm, added respectively in second group of every test tube 30ppm, 60ppm, 90ppm,
The sodium selenite solution of 120ppm, 150ppm, 180ppm, 210ppm, 240ppm, 270ppm divide in every test tube of third group
Not Tian Jia 30ppm, 60ppm, 90ppm, 120ppm, 150ppm, 180ppm, 210ppm, 240ppm, 270ppm sodium selenite it is molten
Liquid, added respectively in the 4th group of every test tube 30ppm, 60ppm, 90ppm, 120ppm, 150ppm, 180ppm, 210ppm,
The sodium selenite solution of 240ppm, 270ppm, added respectively in the 5th group of every test tube 30ppm, 60ppm, 90ppm,
The sodium selenite solution of 120ppm, 150ppm, 180ppm, 210ppm, 240ppm, 270ppm obtain five groups of 9 kinds of various concentrations
Containing seleno culture medium.
(4) contain selenium by what 5 kinds in step (1) candidate mushroom strains were inoculated into (3) five groups of 9 kinds of various concentrations of step respectively
In culture medium：I.e. by the first candidate mushroom strain be seeded to respectively first group containing 30ppm, 60ppm, 90ppm, 120ppm,
The sodium selenite solution of 150ppm, 180ppm, 210ppm, 240ppm, 270ppm contain in seleno culture medium, candidate fragrant by second
Mushroom strains be seeded to respectively second group containing 30ppm, 60ppm, 90ppm, 120ppm, 150ppm, 180ppm, 210ppm, 240ppm,
The sodium selenite solution of 270ppm contains in seleno culture medium, the third candidate's mushroom strain is seeded to third group respectively to be contained
The sodium selenite solution of 30ppm, 60ppm, 90ppm, 120ppm, 150ppm, 180ppm, 210ppm, 240ppm, 270ppm contain
In seleno culture medium, by the 4th kind of candidate mushroom strain be seeded to respectively the 4th group containing 30ppm, 60ppm, 90ppm, 120ppm,
The sodium selenite solution of 150ppm, 180ppm, 210ppm, 240ppm, 270ppm contain in seleno culture medium, candidate fragrant by the 5th kind
Mushroom strains be seeded to respectively the 5th group containing 30ppm, 60ppm, 90ppm, 120ppm, 150ppm, 180ppm, 210ppm, 240ppm,
The sodium selenite solution of 270ppm contains in seleno culture medium, keeps 20-28 DEG C of temperature, humidity 60-70%, PH6-7, culture 12
It.
(5) in observation of steps (4) candidate mushroom strain in various concentration containing the growing state in seleno culture medium, select bacterium
The vigorous healthy and strong, speed of growth of silk growth it is fast be used as first class inoculum, the speed of growth described here refers to that 7-10 days mycelia are long soon
Full inclined-plane, for example, it is most excellent containing the first candidate mushroom strain growing state in seleno culture medium in first group of 150ppm, then it selects
The first candidate mushroom strain is selected as first class inoculum, the concentration of sodium selenite of record culture first class inoculum is as optimal selenous acid
Na concn, such as 150ppm, and preservation first class inoculum.
Step 2: preparing culture material containing selenium：Culture material is made, according to optimal concentration of sodium selenite in step 1, for example is walked
Suddenly the 150ppm of (5) record, 150ppm sodium selenite solutions are added in culture material and are obtained containing selenium (150ppm) culture material；Cultivation
Material includes the raw material components of following weight ratio：Cotton seed hulls 75%, corncob 18%, wheat bran 10%, lime 1.5%, gypsum 1%,
Magnesium sulfate 0.2%, water 65%.
Step 3: first class inoculum (such as the first candidate mushroom strain) is inoculated into containing in selenium (such as 150ppm) culture material,
20-28 DEG C of temperature is kept, humidity 60-70%, PH6-7 grow Se-rich xianggu.
Step 4：Before being reused to the first class inoculum of preservation in step 1, the selenium-rich energy of the first class inoculum of preservation is detected
Power, select mycelia grow the vigorous healthy and strong, speed of growth it is fast be used as first class inoculum again, repeatedly step 2 and step 3.
The selenium rich ability of the detection preservation first class inoculum refers to that preservation first class inoculum is seeded to first class inoculum culture medium
The selenium rich ability of middle detection preservation first class inoculum, select mycelia grow the vigorous healthy and strong, speed of growth soon again as level-one bacterium
Kind.
Step 5：The selenium rich ability for detecting the first class inoculum of preservation to the first class inoculum of selenium rich ability difference purify multiple
It is strong.
Purification and rejuvenation include the following steps：(1) first class inoculum culture medium is made；First class inoculum culture medium is PDA culture medium,
Include the raw material components of following weight ratio per 1000mlPDA culture mediums：Peeled potatoes 200g, glucose 20g, potassium dihydrogen phosphate
1.5g, dipotassium hydrogen phosphate 1.5g, magnesium sulfate 3g, vitamin B11 0mg, agar 20g, remaining is water.(2) selection selenium rich ability is poor
First class inoculum, be seeded in first class inoculum culture medium.(3) 20-28 DEG C of temperature, humidity 60-70%, PH6-7 is kept to cultivate 7-
15 days, select without miscellaneous bacteria, mycelia grow vigorous healthy and strong, strain that the speed of growth is fast to get.
The Se-rich xianggu prepared in the above manner, physical and chemical index can reach following table requirement：
| Project | Index | The method of inspection |
| Selenium/(mg/kg) | 100.0-500.0 | GB 5009.93 |
| Organic Selenium account for total selenium mass percent/(w/%) >= | 95.0 | Test method |
| Protein/(g/100g) >= | 16.0 | GB 5009.5 |
| Dietary fiber/(g/100g) >= | 18.0 | GB 5009.88 |
| Moisture/(g/100g) >= | 11.0 | GB 5009.3 |
| Ash content/(g/100g)≤ | 8.5 | GB 5009.4 |
| Lead (in terms of Pb)/mg/kg≤ | 0.8 | GB 5009.12 |
| Cadmium (in terms of Cd)/mg/kg≤ | 0.5 | GB 5009.15 |
| Total mercury (in terms of Hg)/mg/kg≤ | 0.1 | GB 5009.17 |
| Total arsenic (in terms of As)/mg/kg≤ | 0.5 | GB 5009.11 |
| Pesticide residue | GB 2763 |
Wherein, test method refers to that inorganic selenium is detached with Organic Selenium through being extracted under the conditions of 6mol/L hydrochloric acid water-baths in sample,
With the content of AFS DETERMINATION inorganic selenium.Organic selenium content is the difference of total selenium and inorganic selenium.
Embodiment six
Selenium-rich gold needle mushroom is prepared in accordance with the following steps：
Step 1: acclimation and screening, obtains first class inoculum：
(1) 4 Needle mushroom strains that selection resistance is strong, growth is fast, yield is high, as candidate Needle mushroom strain.
(2) first class inoculum culture medium is made；First class inoculum culture medium is PDA culture medium, is wrapped in every 1000mlPDA culture mediums
Include the raw material components of following weight ratio：Peeled potatoes 200g, glucose 20g, potassium dihydrogen phosphate 1.5g, dipotassium hydrogen phosphate 1.5g,
Magnesium sulfate 3g, vitamin B11 0mg, agar 20g, remaining is water.
(3) prepare 36 test tubes, be divided into four groups i.e. first group, second group, third group and the 4th group by 9 every group of test tubes,
First class inoculum culture medium 8ml is dispensed in first group, second group, third group and the 4th group of test tube respectively, and at first group every
The Asia of 30ppm, 60ppm, 90ppm, 120ppm, 150ppm, 180ppm, 210ppm, 240ppm, 270ppm are added in test tube respectively
Selenic acid sodium solution, added respectively in second group of every test tube 30ppm, 60ppm, 90ppm, 120ppm, 150ppm, 180ppm,
The sodium selenite solution of 210ppm, 240ppm, 270ppm, added respectively in every test tube of third group 30ppm, 60ppm,
The sodium selenite solution of 90ppm, 120ppm, 150ppm, 180ppm, 210ppm, 240ppm, 270ppm, in the 4th group of every examination
The sub- selenium of 30ppm, 60ppm, 90ppm, 120ppm, 150ppm, 180ppm, 210ppm, 240ppm, 270ppm are added in pipe respectively
Acid sodium solution, obtain four groups of 9 kinds of various concentrations contains seleno culture medium.
(4) 4 kinds of candidate Needle mushroom strains in step (1) are inoculated into containing for (3) four groups of 9 kinds of various concentrations of step respectively
In seleno culture medium：I.e. by the first candidate Needle mushroom strain be seeded to respectively first group containing 30ppm, 60ppm, 90ppm,
The sodium selenite solution of 120ppm, 150ppm, 180ppm, 210ppm, 240ppm, 270ppm contain in seleno culture medium, by second
Kind of candidate Needle mushroom strain be seeded to respectively second group containing 30ppm, 60ppm, 90ppm, 120ppm, 150ppm, 180ppm,
The sodium selenite solution of 210ppm, 240ppm, 270ppm contain in seleno culture medium, the third candidate's Needle mushroom strain is connect respectively
It plants to sub- selenium of the third group containing 30ppm, 60ppm, 90ppm, 120ppm, 150ppm, 180ppm, 210ppm, 240ppm, 270ppm
Acid sodium solution containing in seleno culture medium, by the 4th kind of candidate Needle mushroom strain be seeded to respectively the 4th group containing 30ppm, 60ppm,
The sodium selenite solution of 90ppm, 120ppm, 150ppm, 180ppm, 210ppm, 240ppm, 270ppm contain in seleno culture medium,
20-28 DEG C of temperature, humidity 60-70%, PH6-7 is kept to cultivate 15 days.
(5) in observation of steps (4) candidate Needle mushroom strain in various concentration containing the growing state in seleno culture medium, selection
Mycelia grows vigorous conduct first class inoculum healthy and strong, the speed of growth is fast, and the speed of growth described here refers to 7-10 days mycelia soon
Inclined-plane is covered with, such as most excellent containing the third candidate Needle mushroom strain growing state in seleno culture medium in third group 180ppm,
Then select the third candidate Needle mushroom strain as first class inoculum, the concentration of sodium selenite of record culture first class inoculum is as optimal
Concentration of sodium selenite, such as 180ppm, and preservation first class inoculum.
Step 2: preparing culture material containing selenium：Culture material is made, according to optimal concentration of sodium selenite in step 1, for example is walked
Suddenly the 180ppm of (5) record, 180ppm sodium selenite solutions are added in culture material and are obtained containing selenium (180ppm) culture material；Cultivation
Material includes the raw material components of following weight ratio：Cotton seed hulls 75%, corncob 18%, wheat bran 10%, lime 1.5%, gypsum 1%,
Magnesium sulfate 0.2%, water 65%.
Step 3: being inoculated into first class inoculum (such as the third candidate Needle mushroom strain) containing selenium (such as 180ppm) culture material
In, 20-28 DEG C of temperature is kept, humidity 60-70%, PH6-7 grow selenium-rich gold needle mushroom.
Step 4：Before being reused to the first class inoculum of preservation in step 1, the selenium-rich energy of the first class inoculum of preservation is detected
Power, select mycelia grow the vigorous healthy and strong, speed of growth it is fast be used as first class inoculum again, repeatedly step 2 and step 3.
The selenium rich ability of the detection preservation first class inoculum refers to that preservation first class inoculum is seeded to first class inoculum culture medium
The selenium rich ability of middle detection preservation first class inoculum, select mycelia grow the vigorous healthy and strong, speed of growth soon again as level-one bacterium
Kind.
Step 5：The selenium rich ability for detecting the first class inoculum of preservation to the first class inoculum of selenium rich ability difference purify multiple
It is strong.
Purification and rejuvenation include the following steps：(1) first class inoculum culture medium is made；First class inoculum culture medium is PDA culture medium,
Include the raw material components of following weight ratio per 1000mlPDA culture mediums：Peeled potatoes 200g, glucose 20g, potassium dihydrogen phosphate
1.5g, dipotassium hydrogen phosphate 1.5g, magnesium sulfate 3g, vitamin B11 0mg, agar 20g, remaining is water.(2) selection selenium rich ability is poor
First class inoculum, be seeded in first class inoculum culture medium.(3) 20-28 DEG C of temperature, humidity 60-70%, PH6-7 is kept to cultivate 7-
15 days, select without miscellaneous bacteria, mycelia grow vigorous healthy and strong, strain that the speed of growth is fast to get.
The selenium-rich gold needle mushroom prepared in the above manner, physical and chemical index can reach following table requirement：
| Project | Index | The method of inspection |
| Selenium/(mg/kg) | 100.0-500.0 | GB 5009.93 |
| Organic Selenium account for total selenium mass percent/(w/%) >= | 95.0 | Test method |
| Protein/(g/100g) >= | 16.0 | GB 5009.5 |
| Dietary fiber/(g/100g) >= | 18.0 | GB 5009.88 |
| Moisture/(g/100g) >= | 11.0 | GB 5009.3 |
| Ash content/(g/100g)≤ | 8.5 | GB 5009.4 |
| Lead (in terms of Pb)/mg/kg≤ | 0.8 | GB 5009.12 |
| Cadmium (in terms of Cd)/mg/kg≤ | 0.5 | GB 5009.15 |
| Total mercury (in terms of Hg)/mg/kg≤ | 0.1 | GB 5009.17 |
| Total arsenic (in terms of As)/mg/kg≤ | 0.5 | GB 5009.11 |
| Pesticide residue | GB 2763 |
Wherein, test method refers to that inorganic selenium is detached with Organic Selenium through being extracted under the conditions of 6mol/L hydrochloric acid water-baths in sample,
With the content of AFS DETERMINATION inorganic selenium.Organic selenium content is the difference of total selenium and inorganic selenium.
Embodiment seven
Se-rich lucid ganoderma is prepared in accordance with the following steps：
Step 1: acclimation and screening, obtains first class inoculum：
(1) 3 lucidum strains that selection resistance is strong, growth is fast, yield is high, as candidate lucidum strain.
(2) first class inoculum culture medium is made；First class inoculum culture medium is PDA culture medium, is wrapped in every 1000mlPDA culture mediums
Include the raw material components of following weight ratio：Peeled potatoes 200g, glucose 20g, potassium dihydrogen phosphate 1.5g, dipotassium hydrogen phosphate 1.5g,
Magnesium sulfate 3g, vitamin B11 0mg, agar 20g, remaining is water.
(3) prepare 27 test tubes, be divided into three groups i.e. first group, second group and third group by 9 every group of test tubes, respectively the
One group, second group and the interior packing first class inoculum culture medium 8ml of third group test tube, and added respectively in first group of every test tube
The sodium selenite solution of 30ppm, 60ppm, 90ppm, 120ppm, 150ppm, 180ppm, 210ppm, 240ppm, 270ppm,
Added respectively in second group of every test tube 30ppm, 60ppm, 90ppm, 120ppm, 150ppm, 180ppm, 210ppm, 240ppm,
The sodium selenite solution of 270ppm, added respectively in every test tube of third group 30ppm, 60ppm, 90ppm, 120ppm,
The sodium selenite solution of 150ppm, 180ppm, 210ppm, 240ppm, 270ppm, the selenium that contains for obtaining three groups of 9 kinds of various concentrations are trained
Support base.
(4) contain selenium by what 3 kinds in step (1) candidate lucidum strains were inoculated into (3) three groups of 9 kinds of various concentrations of step respectively
In culture medium：I.e. by the first candidate lucidum strain be seeded to respectively first group containing 30ppm, 60ppm, 90ppm, 120ppm,
The sodium selenite solution of 150ppm, 180ppm, 210ppm, 240ppm, 270ppm contain in seleno culture medium, by second of candidate spirit
Sesame strain be seeded to respectively second group containing 30ppm, 60ppm, 90ppm, 120ppm, 150ppm, 180ppm, 210ppm, 240ppm,
The sodium selenite solution of 270ppm contains in seleno culture medium, the third candidate's lucidum strain is seeded to third group respectively to be contained
The sodium selenite solution of 30ppm, 60ppm, 90ppm, 120ppm, 150ppm, 180ppm, 210ppm, 240ppm, 270ppm contain
In seleno culture medium, 20-28 DEG C of temperature, humidity 60-70%, PH6-7 is kept to cultivate 13 days.
(5) in observation of steps (4) candidate lucidum strain in various concentration containing the growing state in seleno culture medium, select bacterium
The vigorous healthy and strong, speed of growth of silk growth it is fast be used as first class inoculum, the speed of growth described here refers to that 7-10 days mycelia are long soon
Full inclined-plane, for example, it is most excellent containing second of candidate lucidum strain growing state in seleno culture medium in second group of 210ppm, then it selects
Second of candidate lucidum strain is selected as first class inoculum, the concentration of sodium selenite of record culture first class inoculum is as optimal selenous acid
Na concn, such as 210ppm, and preservation first class inoculum.
Step 2: preparing culture material containing selenium：Culture material is made, according to optimal concentration of sodium selenite in step 1, for example is walked
Suddenly the 210ppm of (5) record, 210ppm sodium selenite solutions are added in culture material and are obtained containing selenium (210ppm) culture material；Cultivation
Material includes the raw material components of following weight ratio：Cotton seed hulls 75%, corncob 18%, wheat bran 10%, lime 1.5%, gypsum 1%,
Magnesium sulfate 0.2%, water 65%.
Step 3: first class inoculum (such as second candidate lucidum strain) is inoculated into containing in selenium (such as 210ppm) culture material,
20-28 DEG C of temperature is kept, humidity 60-70%, PH6-7 grow Se-rich lucid ganoderma.
Step 4：Before being reused to the first class inoculum of preservation in step 1, the selenium-rich energy of the first class inoculum of preservation is detected
Power, select mycelia grow the vigorous healthy and strong, speed of growth it is fast be used as first class inoculum again, repeatedly step 2 and step 3.
The selenium rich ability of the detection preservation first class inoculum refers to that preservation first class inoculum is seeded to first class inoculum culture medium
The selenium rich ability of middle detection preservation first class inoculum, select mycelia grow the vigorous healthy and strong, speed of growth soon again as level-one bacterium
Kind.
Step 5：The selenium rich ability for detecting the first class inoculum of preservation to the first class inoculum of selenium rich ability difference purify multiple
It is strong.
Purification and rejuvenation include the following steps：(1) first class inoculum culture medium is made；First class inoculum culture medium is PDA culture medium,
Include the raw material components of following weight ratio per 1000mlPDA culture mediums：Peeled potatoes 200g, glucose 20g, potassium dihydrogen phosphate
1.5g, dipotassium hydrogen phosphate 1.5g, magnesium sulfate 3g, vitamin B11 0mg, agar 20g, remaining is water.(2) selection selenium rich ability is poor
First class inoculum, be seeded in first class inoculum culture medium.(3) 20-28 DEG C of temperature, humidity 60-70%, PH6-7 is kept to cultivate 7-
15 days, select without miscellaneous bacteria, mycelia grow vigorous healthy and strong, strain that the speed of growth is fast to get.
The Se-rich lucid ganoderma prepared in the above manner, physical and chemical index can reach following table requirement：
| Project | Index | The method of inspection |
| Selenium/(mg/kg) | 100.0-500.0 | GB 5009.93 |
| Organic Selenium account for total selenium mass percent/(w/%) >= | 95.0 | Test method |
| Protein/(g/100g) >= | 16.0 | GB 5009.5 |
| Dietary fiber/(g/100g) >= | 18.0 | GB 5009.88 |
| Moisture/(g/100g) >= | 11.0 | GB 5009.3 |
| Ash content/(g/100g)≤ | 8.5 | GB 5009.4 |
| Lead (in terms of Pb)/mg/kg≤ | 0.8 | GB 5009.12 |
| Cadmium (in terms of Cd)/mg/kg≤ | 0.5 | GB 5009.15 |
| Total mercury (in terms of Hg)/mg/kg≤ | 0.1 | GB 5009.17 |
| Total arsenic (in terms of As)/mg/kg≤ | 0.5 | GB 5009.11 |
| Pesticide residue | GB 2763 |
Wherein, test method refers to that inorganic selenium is detached with Organic Selenium through being extracted under the conditions of 6mol/L hydrochloric acid water-baths in sample,
With the content of AFS DETERMINATION inorganic selenium.Organic selenium content is the difference of total selenium and inorganic selenium.
Embodiment eight
A kind of selenium-rich gill fungus bacterium strain, according to first class inoculum made from a kind of above-mentioned breeding method of selenium-rich gill fungus bacterium.
Embodiment nine
A kind of selenium-rich gill fungus bacterium, using a kind of above-mentioned selenium-rich gill fungus bacterium that the breeding method of selenium-rich gill fungus bacterium obtains.
The present invention is using the strong first class inoculum of first acclimation and screening selenium rich ability, then expands the cultivation that cultivation obtains selenium-rich gill fungus bacterium
Selenium-rich gill fungus bacterium organic selenium content is promoted to 100-500ppm by method from 0.1ppm, and yield is about the 1000-5000 of existing product
Times.The selenium-rich gill fungus bacterium organic selenium content obtained in this way accounts for 90% or more of total selenium content, and amino acid content is also therewith
It significantly improves.The present invention can improve the organic selenium content in gill fungus bacterium energetically, reduce inorganic selenium, improve production efficiency, avoid poison
Side reaction ensures health.
The above is only a preferred embodiment of the present invention, protection scope of the present invention is not limited merely to above-mentioned implementation
Example, all technical solutions belonged under thinking of the present invention all belong to the scope of protection of the present invention.It should be pointed out that for the art
Those of ordinary skill for, several improvements and modifications without departing from the principles of the present invention, these improvements and modifications
It should be regarded as protection scope of the present invention.
Claims (10)
1. a kind of breeding method of selenium-rich gill fungus bacterium, includes the following steps：
Step 1: acclimation and screening, obtains first class inoculum：Prepare candidate gill fungus bacterium strain, makes first class inoculum culture medium, quantitative separating
First class inoculum culture medium and the sodium selenite solution for adding various concentration wherein respectively, obtain the culture containing selenium of various concentration
Candidate gill fungus bacterium strain is seeded to and cultivates, keeps suitable temperature, humidity and soda acid by base containing in seleno culture medium for various concentration
Degree obtains the strong candidate gill fungus bacterium strain of selenium rich ability by acclimation and screening and is used as first class inoculum, the Asia of record culture first class inoculum
Selenic acid na concn is as optimal concentration of sodium selenite, and preservation first class inoculum；
Step 2: preparing culture material containing selenium：Culture material is made, according to the optimal concentration of sodium selenite in step 1 by sodium selenite
Solution, which is added in culture material, obtains culture material containing selenium；
Step 3: being inoculated into first class inoculum in culture material containing selenium, suitable temperature, humidity and acid-base value are kept, obtains selenium-rich
Gill fungus bacterium.
2. a kind of breeding method of selenium-rich gill fungus bacterium according to claim 1, it is characterised in that：The step 1 includes following
Step：
(1) 2 or more the different gill fungus bacterium strains that selection resistance is strong, growth is fast, yield is high, as candidate gill fungus bacterium strain；
(2) first class inoculum culture medium is made；
(3) quantitative separating first class inoculum culture medium and difference add the sodium selenite solution of various concentration wherein, obtain difference
Concentration contains seleno culture medium；
(4) by the candidate gill fungus bacterium strain in step (1) be inoculated into respectively step (3) various concentration containing in seleno culture medium, keep
20-28 DEG C of temperature, humidity 60-70%, PH6-7 are cultivated 7-15 days；
(5) in various concentration containing the growing state in seleno culture medium, selection mycelia gives birth to candidate gill fungus bacterium strain in observation of steps (4)
The long vigorous healthy and strong, speed of growth it is fast be used as first class inoculum, the concentration of sodium selenite of record culture first class inoculum is as optimal Asia
Selenic acid na concn, and preservation first class inoculum.
3. a kind of breeding method of selenium-rich gill fungus bacterium according to claim 2, it is characterised in that：Adding in the step (3)
Add the sodium selenite solution of various concentration refer to added respectively in first class inoculum culture medium 30ppm, 60ppm, 90ppm,
The sodium selenite solution of 120ppm, 150ppm, 180ppm, 210ppm, 240ppm, 270ppm.
4. a kind of breeding method of selenium-rich gill fungus bacterium according to claim 1, it is characterised in that：Increase step after the step 3
Rapid four：Before being reused to the first class inoculum of preservation in step 1, the selenium rich ability of the first class inoculum of preservation is detected, selects mycelia
Grow the vigorous healthy and strong, speed of growth it is fast be used as first class inoculum again, repeat step 2 and step 3.
5. a kind of breeding method of selenium-rich gill fungus bacterium according to claim 4, it is characterised in that：The detection preservation level-one bacterium
The selenium rich ability of kind refers to that preservation first class inoculum is seeded to the selenium-rich energy that preservation first class inoculum is detected in first class inoculum culture medium
Power, select mycelia grow the vigorous healthy and strong, speed of growth it is fast again as first class inoculum.
6. a kind of breeding method of selenium-rich gill fungus bacterium according to claim 4, it is characterised in that：Increase step after the step 4
Rapid five：The selenium rich ability for detecting the first class inoculum of preservation, purificates and rejuvenates to the first class inoculum of selenium rich ability difference.
7. a kind of breeding method of selenium-rich gill fungus bacterium according to claim 1, it is characterised in that：Level-one in the step 1
Bacterium culture medium is PDA culture medium, includes the raw material components of following weight ratio per 1000mlPDA culture mediums：Peeled potatoes
200g, glucose 20g, potassium dihydrogen phosphate 1.5g, dipotassium hydrogen phosphate 1.5g, magnesium sulfate 3g, vitamin B11 0mg, agar 20g,
Yu Weishui.
8. a kind of breeding method of selenium-rich gill fungus bacterium according to claim 1, it is characterised in that：Cultivation in the step 3
Material includes the raw material components of following weight ratio：Cotton seed hulls 75%, corncob 18%, wheat bran 10%, lime 1.5%, gypsum 1%,
Magnesium sulfate 0.2%, water 65%.
9. a kind of selenium-rich gill fungus bacterium strain, it is characterised in that：A kind of selenium-rich gill fungus bacterium according to any one of claim 1-3
First class inoculum made from breeding method.
10. a kind of selenium-rich gill fungus bacterium, it is characterised in that：Using a kind of training of selenium-rich gill fungus bacterium described in any one of claim 1-8
Educate the selenium-rich gill fungus bacterium that method obtains.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109168977A (en) * | 2018-10-30 | 2019-01-11 | 湖南奇硒健康产业有限公司 | A kind of selenium-rich wheat food and its breeding method |
| CN109566989A (en) * | 2018-10-30 | 2019-04-05 | 湖南奇硒健康产业有限公司 | A kind of direct-edible selenium-rich wheat food and its breeding method |
| CN110337986A (en) * | 2019-07-10 | 2019-10-18 | 杭州丝绸之路文化艺术有限公司 | A kind of Spawn incubation method of Phellinus |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102511305A (en) * | 2011-12-07 | 2012-06-27 | 苏州硒谷科技有限公司 | Method for cultivating high-selenium-tolerability strain of edible fungi |
-
2017
- 2017-01-12 CN CN201710021755.3A patent/CN108611277A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102511305A (en) * | 2011-12-07 | 2012-06-27 | 苏州硒谷科技有限公司 | Method for cultivating high-selenium-tolerability strain of edible fungi |
Non-Patent Citations (2)
| Title |
|---|
| JERZY FALANDYSZ: "Selenium in Edible Mushrooms", 《JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH》 * |
| 王贺祥等: "《食用菌栽培手册》", 31 January 2015 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109168977A (en) * | 2018-10-30 | 2019-01-11 | 湖南奇硒健康产业有限公司 | A kind of selenium-rich wheat food and its breeding method |
| CN109566989A (en) * | 2018-10-30 | 2019-04-05 | 湖南奇硒健康产业有限公司 | A kind of direct-edible selenium-rich wheat food and its breeding method |
| CN110337986A (en) * | 2019-07-10 | 2019-10-18 | 杭州丝绸之路文化艺术有限公司 | A kind of Spawn incubation method of Phellinus |
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Application publication date: 20181002 |