CN107950530A - Application of the cinnamic acid in the preparation for improving plant salt stress resistance is prepared - Google Patents
Application of the cinnamic acid in the preparation for improving plant salt stress resistance is prepared Download PDFInfo
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- CN107950530A CN107950530A CN201711264864.4A CN201711264864A CN107950530A CN 107950530 A CN107950530 A CN 107950530A CN 201711264864 A CN201711264864 A CN 201711264864A CN 107950530 A CN107950530 A CN 107950530A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
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Abstract
The present invention provides application of the cinnamic acid in the preparation for improving plant salt stress resistance is prepared, when the present invention is experimentally confirmed concentration≤20 μM for adding cinnamic acid in the medium, cinnamic acid does not interfere with the root growth of arabidopsis, but works as concentration>At 20 μM, with the increase of concentration, cinnamic acid also strengthens the inhibitory action of root long therewith.Thus, 10 μM of optimum selecting of the present invention is follow-up experimental concentration.After 10 μM of cinnamic acids are added at the same time in 150 mM NaCl culture mediums, salt stress suppresses to weaken to root growth so that economic stress is eased.In salt stress experiment in simulating soil, after 10 μM of cinnamic acids are added in the plant nutrition liquid containing 100 mM NaCl, the salt stress resistance of arabidopsis is significantly increased, improves the survival rate of plant.The present invention is experimentally confirmed cinnamic acid has potential application value in terms of plant salt stress resistance is improved.
Description
Technical field
The invention belongs to technical field of plant protection, and in particular to cinnamic acid improves the system of plant salt stress resistance preparing
Application in agent.
Background technology
The soil salinization is to influence a key factor of agricultural production and ecological environment, due to industrial expansion and population
Be continuously increased, agricultural cultivated area is fewer and fewer, thus improve crop per unit area yield and resistance become the future of agriculture can hold
The important topic that supervention is opened up and environmental improvement is faced.Plant can show different characters, seriously when being subject to environment stress
When will cause death.Salt stress is an important environmental factor for influencing growth and development of plants, therefore the Salt-resistance mechanism of plant needs
Treat further in-depth study.Seed is young after its sprouting and sprouting under salt stress as the most important propagating materials of plant
The early growth situation of seedling reflects the saline-alkaline tolerance of plant to a certain extent, helps to inquire into it in different growing environments
Under the conditions of carry out culture application.
Cinnamic acid（cinnamic acid, CA）, also known as β-cinnamic acid, cinnamic acid, be from cassia or
The organic acid that styrax is isolated.Degraded in plant by phenylalanine deamination and produce cinnamic acid.CA points are cis and trans, suitable
Formula is natural, and trans is synthesis.It is mainly used for essence and flavoring agent, food additives, medical industry, beauty, pesticide, organic synthesis etc.
Aspect.
The content of the invention
For the situation of the above-mentioned prior art, object of the present invention is to provide cinnamic acid to prepare the raising plant salt side of body
Compel the application in the preparation of resistance, the cinnamic acid purchase cost used in the present invention is low, and the remission effect of arabidopsis salt stress is shown
Write, and effect on environment is little.The present invention confirms that external source adds low concentration cinnamic acid first by experiment（Cinnamic
acid,CA）The salt stress resistance of arabidopsis can be improved.
For achieving the above object, the present invention is achieved by the following scheme：
The present invention provides application of the cinnamic acid in the preparation for improving plant salt stress resistance is prepared.
Further：Addition concentration≤20 μM/L of cinnamic acid in the preparation.
Further：When the addition concentration of cinnamic acid is 10 μM/L in the preparation, Plants under Salt Stress root is significantly improved
System's growth.
Further：Concrete application method is：10 μM/L meat is added in the coercing cultivation base containing 150 mM NaCl
Cinnamic acid, can improve arabidopsis Seedling root under salt stress and grow, alleviate the salt stress that seedling is subjected to.
Further：Concrete application method is：10 μM/L meat is added in the plant nutrition liquid containing 100 mM NaCl
Cinnamic acid, significantly improves the salt stress resistance of arabidopsis, improves the survival rate of plant.
Further：The plant is arabidopsis.
Compared with prior art, advantages of the present invention and have the technical effect that：The present invention is had found by experiment of transplanting seedlings, 150
After adding 10 μM of CA at the same time in mM NaCl coercing cultivation bases, inhibitory action of the salt stress to root can be weakened, plan can be improved
Southern mustard WT lines salt stress resistance.Cinnamic acid used in the present invention is cheap, environment-friendly, green ring
Protect, be widely used in life produces.Influence based on salt stress to plant growth, it is thus found that applying the cinnamic acid of low concentration
The salt stress resistance of arabidopsis can be improved.In view of effect of the material in salt stress, it is believed that the material is to improving plant salt
Stress resistance has potential application value.
Brief description of the drawings
Figure 1A is influences of the various concentrations CA to arabidopsis root growth（1/2MS (-), 4 DEG C of laminations three days, 22 DEG C of perpendicular trainings
Growth three days, is transferred to processing board, each 15 plants of culture dish, erects training growth 10 days）.
Figure 1B is the data statistics to root long.
Fig. 2A is different disposal condition：1/2MS (-)、10 μM CA、150 mM NaCl、150 mM NaCl+10 μM
CA （1/2MS (-), 4 DEG C of laminations three days, 22 DEG C of perpendicular training growths three days, are transferred to processing board, each 15 plants of culture dish, erects training life
It is 10 days long）.
Fig. 2 B are the data statistics to root long.
Fig. 3 A are different disposal condition：Control group（Contain only nutrient solution）、10 μM CA、100 mM NaCl、100 mM
NaCl+10 μM of CA, simulates actual Soil salinity stress environment, grows one month.
Fig. 3 B are the data statistics to survival rate.
Embodiment
Technical scheme is further explained in following embodiments, description and embodiment more than, this
Field technology personnel can determine the essential characteristic of the present invention, and without departing from the spirit and scope of the invention, can
To make various changes and modifications to the present invention, so that it is applicable in various uses and condition；Mentioned reagent is added to by the present invention
In model plant arabidopsis solid medium 1/2MS (-).Of the present invention is state of the art.
The present invention have purchased commercialization cinnamic acid first（Cinnamic acid）, need to be equipped to close according to experimental concentration
The mother liquor of suitable concentration（100 mM/L）, it is added in 1/2MS (-) culture medium, is configured to the processing culture of different condition
Base.
Experiment material choose model plant arabidopsis wild type, it is all of above experiment using it is of the same period it is in the same size,
The experiment seed of full seed, ensures the uniformity of experiment material.
Embodiment 1：The selection of concentration for the treatment of
One：The preparation of solid medium containing various concentrations cinnamic acid
The present invention utilizes commercialization cinnamic acid（Cinnamic acid）It is configured to the mother liquor of suitable concentration（100 mM/L）, according to
Experimental concentration needs, and is added in 1/2MS (-) culture medium, is configured to the processing culture medium of different condition, carries out follow-up
Transplant seedlings experiment.
According to experimental considerations, contrived experiment journey is as follows：
1st, the preparation of model plant arabidopsis solid medium
（1）MS culture medium of the selection for Plant Tissue Breeding（Without agar and sucrose）, it is formulated as according to needed for experiment
1/2MS (-) culture medium.Specific formula is as follows：Medicine MS 2.37g/L, sucrose 15g/L are weighed, is dissolved in ultra-pure water and fixed
Hold to 1000 ml, be 1/2MS (-) with 1M KOH solutions and dilute hydrochloric acid tune pH to 5.80.
（2）Dispense：Every 200 ml fluid nutrient mediums are sub-packed in 250 ml triangular flasks, addition 1.7g agaroses, 121 DEG C
High temperature, autoclaving 20 minutes.
（3）After sterilizing, when culture medium is cooled to 50-60 DEG C, added in super-clean bench into culture medium quantitative
CA mother liquors (100 mM/L), are configured to the CA solids treatment culture mediums of concentration gradient, are respectively 0,10,20,30,40,50,60,
70,80,90,100 μM/L CA, are subsequently poured into glass dish, and every 200 ml dispenses 10 glass dishes.
（4）After culture medium solidification, label concentration, it is spare to be sealed in sterile bag.
2nd, seedling prepares
Plant Arabidopsis thaliana wild type in mode（Col-0）To handle material, examination in the same size, full seed of the same period is chosen
Seed is tested, surface disinfection by chlorine 5h, seed single file is layered on 1/2MS (-) solid medium, 4 DEG C of dark laminations three days,
22 DEG C of perpendicular trainings are grown three days.
3rd, transplant seedlings processing：
The consistent seedling of root long is selected to solids treatment culture medium, it is grown in same horizontal line, each 15 plants of culture dish,
22 DEG C of long-day erect training growth 10 days, as shown in Figure 1A.
Root long data and statistical result drawing are measured, as shown in Figure 1B.
Pass through interpretation of result of transplanting seedlings（Shown in Figure 1A and Figure 1B）, addition concentration≤20 μM/L of CA do not influence plant life
It is long, but>Suppress plant strain growth during 20 μM/L.
This example determines the addition concentration range of the reagent C A：≤ 20 μM/L.
Two：Optimal CA applies determining for concentration
1st, prepared by culture medium
（1）MS culture medium of the selection for Plant Tissue Breeding（Without agar and sucrose）, it is formulated as according to needed for experiment
1/2MS culture mediums：1/2MS (-)、150 mM NaCl.Specific formula is as follows：Medicine MS 2.37g/L, sucrose 15g/L are weighed,
It is dissolved in ultra-pure water and is settled to 1000 ml, is 1/2MS (-) with 1M KOH solutions and dilute hydrochloric acid tune pH to 5.80,
The extra addition sodium chloride 58.44g/L in this basis, you can according to 150 mM NaCl culture mediums of required configuration.
（2）Dispense：Every 200 ml is sub-packed in 250 ml triangular flasks, adds 1.7g agaroses, 121 DEG C of high temperature, high pressures
Sterilizing 20 minutes.
（3）After sterilizing, when culture medium is cooled to 50-60 DEG C, added in super-clean bench into culture medium quantitative
CA mother liquors, are configured to different disposal culture medium, are respectively 1/2MS (-), 10 μM of CA, 150 mM NaCl, 150 mM NaCl+
10 μM of CA, are subsequently poured into glass dish, and every 200 ml dispenses 10 glass dishes.
（4）After culture medium solidification, label concentration, it is spare to be sealed in sterile bag.
2nd, seedling prepares
Plant Arabidopsis thaliana wild type in mode（Col-0）To handle material, examination in the same size, full seed of the same period is chosen
Seed is tested, surface disinfection by chlorine 5h, seed single file is layered on 1/2MS (-) solid medium, 4 DEG C of dark laminations three days,
22 DEG C of perpendicular trainings are grown three days.
3rd, transplant seedlings processing：
The consistent seedling of root long is selected to various solids treatment culture mediums, it is grown in same horizontal line, each culture dish
15 plants, 22 DEG C of long-day erect training growth 10 days, as shown in Figure 2 A.
Root long data and statistical result drawing are measured, as shown in Figure 2 B.
Pass through interpretation of result of transplanting seedlings（As shown in Figure 2 A and 2 B）, when CA addition concentration is 10 μM/L, improve under salt stress
The effect of arabidopsis root growth is most obvious.
This example determines the optimal addition concentration of the reagent C A：10 μM/L.
Embodiment 2
Embodiment and experiment effect in conjunction with this experiment, further illustrate the advantageous effects of the present invention.
1st, prepared by culture substrate
（1）Selection for plant growth vermiculite culture substrate, according to needed for experiment prepare plant growth needed for nutrient solution and
100 mM NaCl。
（2）Dispense：It is sub-packed in per 5L nutrient solutions in glassware bottle.
（3）Processing packet：Control group（Contain only nutrient solution）、10 μM CA、100 mM NaCl、100 mM NaCl + 10
μM CA, every kind of processing set three groups of repetitions.
（4）Experiment process：Use control group every time（Contain only nutrient solution）、10 μM CA、100 mM NaCl、100 mM
+ 10 μM of CA submergence culture substrates of NaCl, simulate actual salt stress environment.
2nd, material prepares
Plant Arabidopsis thaliana wild type in mode（Col-0）To handle material, examination in the same size, full seed of the same period is chosen
Seed is tested, seed is layered on vermiculite culture substrate with 3*3.
3rd, survival rate counts
It is one month to choose experimental period, observes the survival rate of arabidopsis under different disposal, as shown in Figure 3A.
Viable count and statistical result drawing are counted, as shown in Figure 3B.As shown in Figure 3A and Figure 3B, 100 mM are being contained
After adding 10 μM/L cinnamic acids in the plant nutrition liquid of NaCl, the salt stress resistance of arabidopsis is significantly improved, improves plant
The survival rate of strain.
The above embodiments are merely illustrative of the technical solutions of the present invention, rather than is limited；Although with reference to foregoing reality
Example is applied the present invention is described in detail, for those of ordinary skill in the art, still can be to foregoing implementation
The technical solution that example is recorded is modified, or carries out equivalent substitution to which part technical characteristic；And these modifications or substitutions,
The essence of appropriate technical solution is not set to depart from the spirit and scope of claimed technical solution of the invention.
Claims (6)
1. application of the cinnamic acid in the preparation for improving plant salt stress resistance is prepared.
2. application of the cinnamic acid according to claim 1 in the preparation for improving plant salt stress resistance is prepared, its feature
It is：Addition concentration≤20 μM/L of cinnamic acid in the preparation.
3. application of the cinnamic acid according to claim 2 in the preparation for improving plant salt stress resistance is prepared, its feature
It is：When the addition concentration of cinnamic acid is 10 μM/L in the preparation, Plants under Salt Stress root growth is significantly improved.
4. application of the cinnamic acid according to claim 1 in the preparation for improving plant salt stress resistance is prepared, its feature
It is：Concrete application method is：10 μM/L cinnamic acids are added in the coercing cultivation base containing 150 mM NaCl, can be improved
Arabidopsis Seedling root is grown under salt stress, alleviates the salt stress that seedling is subjected to.
5. application of the cinnamic acid according to claim 1 in the preparation for improving plant salt stress resistance is prepared, its feature
It is：Concrete application method is：10 μM/L cinnamic acids are added in the plant nutrition liquid containing 100 mM NaCl, are significantly carried
The salt stress resistance of high arabidopsis, improves the survival rate of plant.
6. application of the cinnamic acid according to claim 1 in the preparation for improving plant salt stress resistance is prepared, its feature
It is：The plant is arabidopsis.
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| CN201711264864.4A CN107950530B (en) | 2017-12-05 | 2017-12-05 | Application of the cinnamic acid in the preparation that preparation improves plant salt stress resistance |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115669665A (en) * | 2022-10-13 | 2023-02-03 | 中国科学院东北地理与农业生态研究所 | Application of ferulic acid in promoting root growth and seedling development of arabidopsis thaliana |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104195150A (en) * | 2014-09-25 | 2014-12-10 | 山东大学 | Application of arabidopsis glycosyl transferase gene UGT79B2 in improving salt resistance and drought resistance of plants |
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- 2017-12-05 CN CN201711264864.4A patent/CN107950530B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104195150A (en) * | 2014-09-25 | 2014-12-10 | 山东大学 | Application of arabidopsis glycosyl transferase gene UGT79B2 in improving salt resistance and drought resistance of plants |
Non-Patent Citations (2)
| Title |
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| 李雪峤等: "《盐胁迫下苯丙烯酸对黄瓜幼苗生长及根际土壤酶活性的影响》", 《中国蔬菜》 * |
| 王学征等: "《盐胁迫下苯丙烯酸对黄瓜幼苗生理特性的影响》", 《沈阳农业大学学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115669665A (en) * | 2022-10-13 | 2023-02-03 | 中国科学院东北地理与农业生态研究所 | Application of ferulic acid in promoting root growth and seedling development of arabidopsis thaliana |
| CN115669665B (en) * | 2022-10-13 | 2023-12-12 | 中国科学院东北地理与农业生态研究所 | Application of ferulic acid in promoting root growth and seedling development of Arabidopsis thaliana |
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Granted publication date: 20181123 Termination date: 20211205 |