LU500595B1 - miR-16 and miR-30c joint Expression Vector, Construction Method and their Use - Google Patents
miR-16 and miR-30c joint Expression Vector, Construction Method and their Use Download PDFInfo
- Publication number
- LU500595B1 LU500595B1 LU500595A LU500595A LU500595B1 LU 500595 B1 LU500595 B1 LU 500595B1 LU 500595 A LU500595 A LU 500595A LU 500595 A LU500595 A LU 500595A LU 500595 B1 LU500595 B1 LU 500595B1
- Authority
- LU
- Luxembourg
- Prior art keywords
- mir
- neural stem
- expression vector
- stem cells
- alzheimer
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7088—Compounds having three or more nucleosides or nucleotides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/14—Type of nucleic acid interfering N.A.
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/14—Type of nucleic acid interfering N.A.
- C12N2310/141—MicroRNAs, miRNAs
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/50—Physical structure
- C12N2310/53—Physical structure partially self-complementary or closed
- C12N2310/531—Stem-loop; Hairpin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
- C12N2740/15041—Use of virus, viral particle or viral elements as a vector
- C12N2740/15043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16041—Use of virus, viral particle or viral elements as a vector
- C12N2740/16043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/20—Vector systems having a special element relevant for transcription transcription of more than one cistron
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicinal Chemistry (AREA)
- Neurology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Neurosurgery (AREA)
- Plant Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Psychiatry (AREA)
- Hospice & Palliative Care (AREA)
- Virology (AREA)
- Epidemiology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202010735788.6A CN111850047B (zh) | 2020-07-28 | 2020-07-28 | 一种miR-16与miR-30c联合表达载体及其构建方法与应用 |
Publications (1)
Publication Number | Publication Date |
---|---|
LU500595B1 true LU500595B1 (en) | 2022-01-28 |
Family
ID=72947907
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
LU500595A LU500595B1 (en) | 2020-07-28 | 2020-11-06 | miR-16 and miR-30c joint Expression Vector, Construction Method and their Use |
Country Status (3)
Country | Link |
---|---|
CN (1) | CN111850047B (fr) |
LU (1) | LU500595B1 (fr) |
WO (1) | WO2022021660A1 (fr) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111850047B (zh) * | 2020-07-28 | 2022-08-12 | 枣庄学院 | 一种miR-16与miR-30c联合表达载体及其构建方法与应用 |
Family Cites Families (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103866018B (zh) * | 2005-08-01 | 2016-05-25 | 俄亥俄州立大学研究基金会 | 用于乳腺癌的诊断、预后和治疗的基于MicroRNA的方法和组合物 |
CN101296702B (zh) * | 2005-09-12 | 2012-11-28 | 俄亥俄州立大学研究基金会 | 用于诊断或治疗bcl2相关癌症的组合物和方法 |
CN101787373B (zh) * | 2009-01-23 | 2013-06-19 | 中国人民解放军第二军医大学东方肝胆外科医院 | 一种携带外源基因在包装细胞中高效生产的重组病毒载体、其构建方法及其用途 |
EP2437750A4 (fr) * | 2009-05-20 | 2014-11-05 | Univ Texas | Identification de micro-arn impliqués dans un remodelage post-infarctus du myocarde et une insuffisance cardiaque |
JP2015509366A (ja) * | 2012-02-22 | 2015-03-30 | ブレインステム バイオテック リミテッド | 神経幹細胞および運動ニューロンの生成 |
EP2833893B1 (fr) * | 2012-04-03 | 2018-08-29 | Reneuron Limited | Microparicules de cellules souches |
WO2014016817A2 (fr) * | 2012-07-17 | 2014-01-30 | Universite De Geneve | Acides nucléiques pour la régulation à la baisse de l'expression génique |
CA2879322A1 (fr) * | 2012-07-19 | 2014-01-23 | Reneuron Limited | Microparticules de cellules souches |
CN103667347A (zh) * | 2012-09-08 | 2014-03-26 | 刘佳 | 一种高效表达抗癌基因的受特定miRNA调控的在胶质瘤细胞中特异增殖的腺病毒及其用途 |
US11293064B2 (en) * | 2013-05-02 | 2022-04-05 | Thomas Jefferson University | Human miRNAs for use in diagnosis, prognosis, and therapy of human conditions and diseases |
WO2016150475A1 (fr) * | 2015-03-22 | 2016-09-29 | Universite De Liege | Micro-arn circulants pour diagnostiquer le cancer du sein |
JP2019514416A (ja) * | 2016-05-16 | 2019-06-06 | エグゾステム バイオテック リミテッド | 間葉系幹細胞と筋損傷及び筋肉関連疾患の治療のための間葉系幹細胞の使用 |
CN111093769A (zh) * | 2017-08-01 | 2020-05-01 | 英联邦高等教育系统天普大学 | 来源于皮质骨干细胞的外来体可以增强心脏损伤后的心脏功能 |
CN111850047B (zh) * | 2020-07-28 | 2022-08-12 | 枣庄学院 | 一种miR-16与miR-30c联合表达载体及其构建方法与应用 |
-
2020
- 2020-07-28 CN CN202010735788.6A patent/CN111850047B/zh active Active
- 2020-11-06 LU LU500595A patent/LU500595B1/en active IP Right Grant
- 2020-11-06 WO PCT/CN2020/127155 patent/WO2022021660A1/fr active Application Filing
Also Published As
Publication number | Publication date |
---|---|
CN111850047B (zh) | 2022-08-12 |
WO2022021660A1 (fr) | 2022-02-03 |
CN111850047A (zh) | 2020-10-30 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Oka et al. | VEGF promotes tumorigenesis and angiogenesis of human glioblastoma stem cells | |
Liu et al. | Ascl1 converts dorsal midbrain astrocytes into functional neurons in vivo | |
RU2535365C2 (ru) | Способы получения и применения мультипотентных клеточных популяций | |
CA2939535A1 (fr) | Utilisation d'un alphavirus dans la preparation de medicaments antitumoraux | |
Meng et al. | Induction of retinal ganglion-like cells from fibroblasts by adenoviral gene delivery | |
CN105492597A (zh) | 利用hmga2制备由非神经元细胞重编程的诱导神经干细胞的方法 | |
Xue et al. | Nestin expression in Müller glial cells in postnatal rat retina and its upregulation following optic nerve transection | |
WO2023169092A1 (fr) | Car-monocyte/macrophage régulé par le micro-environnement tumoral, son procédé de préparation et son utilisation | |
LU500595B1 (en) | miR-16 and miR-30c joint Expression Vector, Construction Method and their Use | |
Deng et al. | Mash-1 modified neural stem cells transplantation promotes neural stem cells differentiation into neurons to further improve locomotor functional recovery in spinal cord injury rats | |
Zhai et al. | Overexpressed ski efficiently promotes neurorestoration, increases neuronal regeneration, and reduces astrogliosis after traumatic brain injury | |
US20090227023A1 (en) | DE-DIFFERENTIATION OF ASTROCYTES INTO NEURAL STEM CELL USING Shh | |
Masjkur et al. | Neurovascular signals suggest a propagation mechanism for endogenous stem cell activation along blood vessels | |
Gao et al. | Differentiation of GDNF and NT-3 dual gene-modified rat bone marrow mesenchymal stem cells into enteric neuron-like cells | |
CN108866100A (zh) | 一种高效率的基因编辑方法 | |
CN105535992B (zh) | Ascl1在诱导星形胶质细胞转分化为功能性神经元中的应用 | |
CN111676222A (zh) | 抑制Mettl3基因表达的shRNA及其重组腺相关病毒与应用 | |
US20240026379A1 (en) | Functional fragment for reprogramming, composition, and application thereof | |
IT201600093825A1 (it) | Composizione farmaceutica di miRNA e suoi usi terapeutici. | |
WO2021228050A1 (fr) | Procédé d'induction d'une transdifférenciation de cellules gliales en neurones fonctionnels, et application associée | |
KR101548616B1 (ko) | 중간엽 줄기세포를 gaβa성 신경세포로 분화시키는 방법 및 bdnf를 포함하는 gaβa성 신경세포 분화유도용 조성물 | |
CN104531709A (zh) | 抑制肿瘤生长与转移的siRNA双干扰组合物及其应用 | |
WO2021055081A1 (fr) | Tissus de type muscle endothéliaux et lisses produits à partir de cellules urinaires et utilisations associées | |
CN108795987A (zh) | 一种FerritinH、Bcl2及EGFP基因联合修饰的神经干细胞制备方法及应用 | |
CN113652428B (zh) | 核膜蛋白敲降在干细胞移植方面的应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG | Patent granted |
Effective date: 20220128 |