LT2208067T - Rezonansinės energijos pernašos testas su skėlimo seka ir skirtuku - Google Patents

Rezonansinės energijos pernašos testas su skėlimo seka ir skirtuku

Info

Publication number
LT2208067T
LT2208067T LTEP08742464.4T LT08742464T LT2208067T LT 2208067 T LT2208067 T LT 2208067T LT 08742464 T LT08742464 T LT 08742464T LT 2208067 T LT2208067 T LT 2208067T
Authority
LT
Lithuania
Prior art keywords
spacer
energy transfer
resonance energy
cleavage sequence
transfer assay
Prior art date
Application number
LTEP08742464.4T
Other languages
English (en)
Inventor
Robert D. Fish
Min Dong
Original Assignee
Biomadison, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biomadison, Inc. filed Critical Biomadison, Inc.
Publication of LT2208067T publication Critical patent/LT2208067T/lt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
    • G01N33/9406—Neurotransmitters
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6432—Quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/952—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from bacteria

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Wood Science & Technology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Engineering & Computer Science (AREA)
  • Toxicology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
LTEP08742464.4T 2007-09-14 2008-03-31 Rezonansinės energijos pernašos testas su skėlimo seka ir skirtuku LT2208067T (lt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US97267307P 2007-09-14 2007-09-14
PCT/US2008/004252 WO2009035476A1 (en) 2007-09-14 2008-03-31 Resonance energy transfer assay with cleavage sequence and spacer

Publications (1)

Publication Number Publication Date
LT2208067T true LT2208067T (lt) 2019-03-12

Family

ID=40452297

Family Applications (1)

Application Number Title Priority Date Filing Date
LTEP08742464.4T LT2208067T (lt) 2007-09-14 2008-03-31 Rezonansinės energijos pernašos testas su skėlimo seka ir skirtuku

Country Status (9)

Country Link
US (6) US9303284B2 (lt)
EP (1) EP2208067B1 (lt)
JP (1) JP5412689B2 (lt)
KR (1) KR101226604B1 (lt)
CN (2) CN105907718B (lt)
CA (1) CA2699612C (lt)
ES (1) ES2717354T3 (lt)
LT (1) LT2208067T (lt)
WO (1) WO2009035476A1 (lt)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7374896B2 (en) * 2001-08-28 2008-05-20 Allergan, Inc. GFP-SNAP25 fluorescence release assay for botulinum neurotoxin protease activity
US8022172B2 (en) * 2001-08-28 2011-09-20 Allergan, Inc. Luminescence resonance energy transfer (LRET) assays for clostridial toxin activity
US9303284B2 (en) * 2007-09-14 2016-04-05 Biomadison, Inc. Resonance energy transfer assay with cleavage sequence and spacer
LT3067696T (lt) * 2009-10-16 2018-09-10 Biomadison, Inc. Genetiškai modifikuotos ląstelės, skirtos rezonansiniam energijos perdavimo tyrimui su sinaptobrevino substrato dalimi
US10246492B2 (en) 2009-10-16 2019-04-02 Biomadison, Inc. Botulinum assay with synaptobrevin substrate moiety
KR20130111954A (ko) * 2010-06-11 2013-10-11 시냅틱 리서치, 엘엘씨 N-단부 지배 프로테아제 활성 표시 방법 및 이의 용도
BR112013030964B1 (pt) * 2011-06-01 2022-08-16 Biomadison, Inc Ensaio botulínico de não fret
US10908146B2 (en) 2011-06-01 2021-02-02 Biomadison, Inc. Compositions and methods for improving sensitivity in cell based assays
US11325954B2 (en) 2011-06-01 2022-05-10 Biomadison, Inc. Compositions and methods for stability testing of botulinum toxin
US9303285B2 (en) 2012-01-04 2016-04-05 Biomadison, Inc. Methods and compounds for increasing sensitivity of botulinum assays
EP2734635A1 (en) 2011-07-19 2014-05-28 ETH Zürich Means and methods for determining clostridial neurotoxins
LT2800973T (lt) * 2012-01-04 2018-09-10 Biomadison, Inc. Būdai ir junginiai, skirti botulino tyrimų jautrumo padidinimui
GB2514825B (en) * 2013-06-06 2017-11-08 Bangor Univ Sensors
EP3030905B9 (en) 2013-08-09 2024-04-03 Biomadison, Inc. Botulinum toxin assay with improved sensitivity
PL405046A1 (pl) * 2013-08-12 2015-02-16 Instytut Biologii Doświadczalnej Im. Marcelego Nenckiego Pan Genetycznie kodowany oparty na FRET biosensor aktywności MMP-9 i jego zastosowanie
EP3047033B1 (en) * 2013-09-17 2018-08-22 Mcmaster University Method for assaying a protease
US9365659B2 (en) * 2014-01-29 2016-06-14 Excelsior Nanotech Corporation System and method for optimizing the efficiency of photo-polymerization
US10634683B2 (en) 2014-11-21 2020-04-28 Merz Pharma Gmbh & Co. Kgaa Methods for the determination of the biological activities of neurotoxin polypeptides
DE102015101668A1 (de) * 2015-02-05 2016-08-11 Benteler Automobiltechnik Gmbh Zweifach fallendes Heiz- und Formwerkzeug sowie Verfahren zur Herstellung warmumgeformter und pressgehärteter Kraftfahrzeugbauteile
CN106442969B (zh) * 2016-08-23 2018-08-31 中国人民解放军军事医学科学院微生物流行病研究所 一种用于检测肉毒毒素的试剂盒
EP3438649B1 (en) * 2017-07-31 2020-03-11 Vestel Elektronik Sanayi ve Ticaret A.S. Identification tag and method of identifying an object
WO2019118837A1 (en) * 2017-12-15 2019-06-20 The Regents Of The University Of Colorado, A Body Corporate Synthetic fluorescent protein biosensors and use thereof in drug screening methods
WO2020168061A1 (en) * 2019-02-13 2020-08-20 Biomadison, Inc. Compositions and methods for protease screening
AR119109A1 (es) * 2019-06-07 2021-11-24 Synaptic Res Llc Células genéticamente manipuladas
WO2021028868A1 (en) * 2019-08-13 2021-02-18 Synaptic Research, Llc Cells highly sensitive to clostridial neurotoxin

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4444879A (en) * 1981-01-29 1984-04-24 Science Research Center, Inc. Immunoassay with article having support film and immunological counterpart of analyte
US6803188B1 (en) * 1996-01-31 2004-10-12 The Regents Of The University Of California Tandem fluorescent protein constructs
US5965699A (en) 1996-11-06 1999-10-12 The United States Of America As Represented By The Secretary Of The Army Assay for the proteolytic activity of serotype a from clostridium botulinum
AU1120200A (en) * 1998-10-16 2000-05-08 Board Of Trustees Of The Leland Stanford Junior University Fluorescent dye binding peptides
US7332567B2 (en) 2001-08-28 2008-02-19 Allergan, Inc. Fret protease assays for clostridial toxins
US7208285B2 (en) 2001-08-28 2007-04-24 Allergan, Inc. Fret protease assays for botulinum serotype A/E toxins
US7374896B2 (en) * 2001-08-28 2008-05-20 Allergan, Inc. GFP-SNAP25 fluorescence release assay for botulinum neurotoxin protease activity
US20080064054A1 (en) 2001-08-28 2008-03-13 Ester Fernandez-Salas Fluorescence resonance energy transfer (fret) assays for clostridial toxin activity
US6504006B1 (en) 2001-10-12 2003-01-07 Nancy Rose Shine Substrate peptides and assays for detecting and measuring proteolytic activity of serotype A neurotoxin from clostridium botulinum
US7183066B2 (en) * 2002-09-27 2007-02-27 Allergan, Inc. Cell-based fluorescence resonance energy transfer (FRET) assays for clostridial toxins
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US20040191887A1 (en) 2002-10-31 2004-09-30 Chapman Edwin Raymond Botulinum neurotoxin B receptors and use thereof
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US9303284B2 (en) * 2007-09-14 2016-04-05 Biomadison, Inc. Resonance energy transfer assay with cleavage sequence and spacer
LT3067696T (lt) 2009-10-16 2018-09-10 Biomadison, Inc. Genetiškai modifikuotos ląstelės, skirtos rezonansiniam energijos perdavimo tyrimui su sinaptobrevino substrato dalimi

Also Published As

Publication number Publication date
CA2699612A1 (en) 2009-03-19
EP2208067A1 (en) 2010-07-21
JP5412689B2 (ja) 2014-02-12
KR20100080530A (ko) 2010-07-08
US20150176051A1 (en) 2015-06-25
CN105907718A (zh) 2016-08-31
US9988666B2 (en) 2018-06-05
US8969016B2 (en) 2015-03-03
ES2717354T3 (es) 2019-06-20
EP2208067A4 (en) 2010-12-22
CN101932936B (zh) 2016-04-27
US20170283851A1 (en) 2017-10-05
US20130095513A1 (en) 2013-04-18
WO2009035476A1 (en) 2009-03-19
CA2699612C (en) 2013-01-08
US9677113B2 (en) 2017-06-13
US10526637B2 (en) 2020-01-07
US20160177369A1 (en) 2016-06-23
JP2010539477A (ja) 2010-12-16
US20110033866A1 (en) 2011-02-10
EP2208067B1 (en) 2019-02-06
US9249451B2 (en) 2016-02-02
US9303284B2 (en) 2016-04-05
CN105907718B (zh) 2020-10-27
CN101932936A (zh) 2010-12-29
KR101226604B1 (ko) 2013-01-28
US20180251812A1 (en) 2018-09-06

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