PL2488868T3 - Oznaczenie transferu energii rezonansu za pomocą cząsteczki substratu synaptobrewiny - Google Patents
Oznaczenie transferu energii rezonansu za pomocą cząsteczki substratu synaptobrewinyInfo
- Publication number
- PL2488868T3 PL2488868T3 PL10824166.2T PL10824166T PL2488868T3 PL 2488868 T3 PL2488868 T3 PL 2488868T3 PL 10824166 T PL10824166 T PL 10824166T PL 2488868 T3 PL2488868 T3 PL 2488868T3
- Authority
- PL
- Poland
- Prior art keywords
- synaptobrevin
- energy transfer
- resonance energy
- transfer assay
- substrate moiety
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
- G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
- G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
- G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
- G01N2333/96427—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
- G01N2333/9643—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
- G01N2333/96486—Metalloendopeptidases (3.4.24)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US25231509P | 2009-10-16 | 2009-10-16 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| PL2488868T3 true PL2488868T3 (pl) | 2016-09-30 |
Family
ID=43876579
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PL10824166.2T PL2488868T3 (pl) | 2009-10-16 | 2010-10-15 | Oznaczenie transferu energii rezonansu za pomocą cząsteczki substratu synaptobrewiny |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US9453254B2 (pl) |
| EP (2) | EP2488868B1 (pl) |
| DK (1) | DK2488868T3 (pl) |
| ES (2) | ES2676277T3 (pl) |
| LT (1) | LT3067696T (pl) |
| PL (1) | PL2488868T3 (pl) |
| WO (1) | WO2011047265A1 (pl) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101932936B (zh) | 2007-09-14 | 2016-04-27 | 拜奥麦迪逊公司 | 利用切割序列和间隔子的共振能量转移测定 |
| US10246492B2 (en) * | 2009-10-16 | 2019-04-02 | Biomadison, Inc. | Botulinum assay with synaptobrevin substrate moiety |
| EP2527434B1 (en) * | 2010-01-22 | 2017-08-09 | Japan Science and Technology Agency | Probe reagent for measurement of proteolytic activity |
| JP6156954B2 (ja) * | 2012-11-21 | 2017-07-05 | イプセン バイオイノベーション リミテッド | タンパク分解性にプロセシングされたポリペプチドの製造方法 |
| LT3014267T (lt) * | 2013-06-28 | 2018-12-10 | Merz Pharma Gmbh & Co. Kgaa | Priemonės ir būdai, skirti neurotoksinių polipeptidų nustatymui |
| EP3060677B1 (en) * | 2013-10-25 | 2018-07-04 | Biomadison, Inc. | Enzyme assay with duplicate fluorophores |
| AU2015301737B2 (en) | 2014-08-12 | 2021-01-28 | Biomadison, Inc. | Botulinum neurotoxins with modified light chain specifity and methods for producing same |
| GB201702500D0 (en) | 2017-02-16 | 2017-04-05 | Univ Sheffield | Stable vamp reporter assay |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001512316A (ja) | 1997-02-13 | 2001-08-21 | メモリアル・スローンケタリング・キャンサー・センター | 細胞微環境における変化を光学的に検出する混成分子 |
| US8022172B2 (en) | 2001-08-28 | 2011-09-20 | Allergan, Inc. | Luminescence resonance energy transfer (LRET) assays for clostridial toxin activity |
| US7332567B2 (en) * | 2001-08-28 | 2008-02-19 | Allergan, Inc. | Fret protease assays for clostridial toxins |
| US8137924B2 (en) | 2003-12-19 | 2012-03-20 | Wisconsin Alumni Research Foundation | Method and compositions for detecting botulinum neurotoxin |
| EP2293064B1 (en) * | 2005-04-05 | 2012-04-04 | Allergan, Inc. | Lipophilic dye-based FRET assays for clostridial toxin activity |
| CN101932936B (zh) * | 2007-09-14 | 2016-04-27 | 拜奥麦迪逊公司 | 利用切割序列和间隔子的共振能量转移测定 |
-
2010
- 2010-10-15 LT LTEP16159320.7T patent/LT3067696T/lt unknown
- 2010-10-15 EP EP10824166.2A patent/EP2488868B1/en active Active
- 2010-10-15 DK DK10824166.2T patent/DK2488868T3/en active
- 2010-10-15 PL PL10824166.2T patent/PL2488868T3/pl unknown
- 2010-10-15 US US13/502,357 patent/US9453254B2/en active Active
- 2010-10-15 ES ES16159320.7T patent/ES2676277T3/es active Active
- 2010-10-15 EP EP16159320.7A patent/EP3067696B1/en active Active
- 2010-10-15 WO PCT/US2010/052847 patent/WO2011047265A1/en active Application Filing
- 2010-10-15 ES ES10824166.2T patent/ES2576747T3/es active Active
Also Published As
| Publication number | Publication date |
|---|---|
| US20120322092A1 (en) | 2012-12-20 |
| WO2011047265A1 (en) | 2011-04-21 |
| EP2488868B1 (en) | 2016-03-30 |
| LT3067696T (lt) | 2018-09-10 |
| ES2576747T3 (es) | 2016-07-11 |
| ES2676277T3 (es) | 2018-07-18 |
| US9453254B2 (en) | 2016-09-27 |
| WO2011047265A4 (en) | 2011-06-16 |
| EP2488868A1 (en) | 2012-08-22 |
| EP3067696B1 (en) | 2018-06-06 |
| DK2488868T3 (en) | 2016-05-09 |
| EP2488868A4 (en) | 2013-10-02 |
| EP3067696A1 (en) | 2016-09-14 |
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