LT2014082A - Didelio tikslumo restrikcijos endonukleazės - Google Patents
Didelio tikslumo restrikcijos endonukleazėsInfo
- Publication number
- LT2014082A LT2014082A LT2014082A LT2014082A LT2014082A LT 2014082 A LT2014082 A LT 2014082A LT 2014082 A LT2014082 A LT 2014082A LT 2014082 A LT2014082 A LT 2014082A LT 2014082 A LT2014082 A LT 2014082A
- Authority
- LT
- Lithuania
- Prior art keywords
- restriction endonucleases
- high fidelity
- fidelity restriction
- mutant enzymes
- buffer
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/21—Endodeoxyribonucleases producing 5'-phosphomonoesters (3.1.21)
- C12Y301/21004—Type II site-specific deoxyribonuclease (3.1.21.4)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
- G01N2333/922—Ribonucleases (RNAses); Deoxyribonucleases (DNAses)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Pateikiami mutantinių fermentų su susilpnintu žvaigždiniu aktyvumu gavimo genų inžineriniai metodai ir kompozicijos, kur mutantiniai fermentai apibrėžtame buferyje turi didesnį tikslumo indeksą (FI), negu nemutuotų fermentų FI tokiame pačiame buferyje.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US30166610P | 2010-02-05 | 2010-02-05 | |
| US38780010P | 2010-09-29 | 2010-09-29 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| LT2014082A true LT2014082A (lt) | 2015-02-25 |
| LT6169B LT6169B (lt) | 2015-06-25 |
Family
ID=43901662
Family Applications (5)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| LT2014081A LT6168B (lt) | 2010-02-05 | 2012-08-28 | Didelio tikslumo restrikcijos endonukleazės |
| LT2014079A LT6166B (lt) | 2010-02-05 | 2012-08-28 | Didelio tikslumo restrikcijos endonukleazės |
| LT2014078A LT6165B (lt) | 2010-02-05 | 2012-08-28 | Didelio tikslumo restrikcijos endonukleazės |
| LT2014082A LT6169B (lt) | 2010-02-05 | 2012-08-28 | Didelio tikslumo restrikcijos endonukleazės |
| LT2014080A LT6167B (lt) | 2010-02-05 | 2012-08-28 | Didelio tikslumo restrikcijos endonukleazės |
Family Applications Before (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| LT2014081A LT6168B (lt) | 2010-02-05 | 2012-08-28 | Didelio tikslumo restrikcijos endonukleazės |
| LT2014079A LT6166B (lt) | 2010-02-05 | 2012-08-28 | Didelio tikslumo restrikcijos endonukleazės |
| LT2014078A LT6165B (lt) | 2010-02-05 | 2012-08-28 | Didelio tikslumo restrikcijos endonukleazės |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| LT2014080A LT6167B (lt) | 2010-02-05 | 2012-08-28 | Didelio tikslumo restrikcijos endonukleazės |
Country Status (7)
| Country | Link |
|---|---|
| US (9) | US8637291B2 (lt) |
| JP (4) | JP6478441B2 (lt) |
| CN (4) | CN116064719A (lt) |
| DE (4) | DE112011106149B3 (lt) |
| GB (6) | GB2521069B (lt) |
| LT (5) | LT6168B (lt) |
| WO (1) | WO2011097590A2 (lt) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6565542B2 (ja) * | 2015-09-25 | 2019-08-28 | トヨタ自動車株式会社 | 半導体装置 |
| JP2020530282A (ja) * | 2017-07-07 | 2020-10-22 | アレナ ファーマシューティカルズ， インコーポレイテッド | 組換えウリカーゼ酵素 |
| CN110241097B (zh) * | 2019-05-24 | 2022-05-17 | 山东大学 | Pet降解酶突变体及其应用 |
| CN110643560A (zh) * | 2019-10-24 | 2020-01-03 | 江苏海洋大学 | 用于重组表达限制性内切酶的工程菌株及其构建方法 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0822223B2 (ja) * | 1988-12-19 | 1996-03-06 | 東洋紡績株式会社 | ＰｖｕＩ制限エンドヌクレアーゼの製造方法 |
| US5470740A (en) * | 1993-11-04 | 1995-11-28 | Life Technologies, Inc. | Cloned NsiI restriction-modification system |
| EP2548953A1 (en) * | 2005-08-04 | 2013-01-23 | New England Biolabs, Inc. | Novel restriction endonucleases, DNA encoding these endonucleases and methods for identifying new endonucleases with the same or varied specificity |
| ATE493493T1 (de) * | 2005-08-31 | 2011-01-15 | New England Biolabs Inc | Kpni-restriktionsendonukleasen mit reduzierter star-aktivität |
| CA2646253A1 (en) * | 2006-04-14 | 2007-10-25 | New England Biolabs, Inc. | Method for cloning and expression of nrui restriction endonuclease |
| EP2390317B1 (en) * | 2007-07-12 | 2014-06-25 | New England Biolabs, Inc. | High fidelity restriction endonucleases |
| US20090060165A1 (en) | 2007-08-30 | 2009-03-05 | Pradeep Kumar Dani | Method and System for Customer Transaction Request Routing |
| WO2011028841A1 (en) * | 2009-09-04 | 2011-03-10 | New England Biolabs, Inc. | Restriction endonuclease variants with altered cleavage specificity and/or reduction of star activity |
-
2011
- 2011-02-07 CN CN202211325032.XA patent/CN116064719A/zh active Pending
- 2011-02-07 GB GB1503419.2A patent/GB2521069B/en active Active
- 2011-02-07 WO PCT/US2011/023944 patent/WO2011097590A2/en active Application Filing
- 2011-02-07 CN CN201811121878.5A patent/CN109706217B/zh active Active
- 2011-02-07 US US13/022,561 patent/US8637291B2/en active Active
- 2011-02-07 GB GB1503416.8A patent/GB2521068B/en active Active
- 2011-02-07 DE DE112011106149.8T patent/DE112011106149B3/de active Active
- 2011-02-07 DE DE112011105841.1T patent/DE112011105841B3/de active Active
- 2011-02-07 GB GB1503422.6A patent/GB2521070B/en active Active
- 2011-02-07 GB GB1420134.7A patent/GB2520434B/en active Active
- 2011-02-07 GB GB1215859.8A patent/GB2490468B/en active Active
- 2011-02-07 CN CN202211453670.XA patent/CN116064720A/zh active Pending
- 2011-02-07 DE DE112011100467.2T patent/DE112011100467B4/de active Active
- 2011-02-07 GB GB1420137.0A patent/GB2518074B/en active Active
- 2011-02-07 CN CN2011800170168A patent/CN102834510A/zh active Pending
- 2011-02-07 JP JP2012552141A patent/JP6478441B2/ja active Active
- 2011-02-07 DE DE112011105840.3T patent/DE112011105840B3/de active Active
-
2012
- 2012-08-28 LT LT2014081A patent/LT6168B/lt unknown
- 2012-08-28 LT LT2014079A patent/LT6166B/lt unknown
- 2012-08-28 LT LT2014078A patent/LT6165B/lt unknown
- 2012-08-28 LT LT2014082A patent/LT6169B/lt unknown
- 2012-08-28 LT LT2014080A patent/LT6167B/lt not_active IP Right Cessation
-
2013
- 2013-12-20 US US14/137,583 patent/US20140154676A1/en not_active Abandoned
- 2013-12-20 US US14/137,636 patent/US20140154779A1/en not_active Abandoned
- 2013-12-20 US US14/137,660 patent/US9150901B2/en active Active
- 2013-12-20 US US14/137,608 patent/US9200310B2/en active Active
-
2015
- 2015-09-18 JP JP2015185099A patent/JP6134758B2/ja active Active
- 2015-11-02 JP JP2015215456A patent/JP6410701B2/ja active Active
-
2016
- 2016-04-19 US US15/133,093 patent/US9856464B2/en active Active
-
2017
- 2017-07-21 JP JP2017141923A patent/JP6509963B2/ja active Active
- 2017-11-28 US US15/824,413 patent/US10294464B2/en active Active
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2019
- 2019-03-15 US US16/355,464 patent/US11001818B2/en active Active
-
2021
- 2021-04-09 US US17/226,693 patent/US12060587B2/en active Active
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| BB1A | Patent application published |
Effective date: 20150225 |
|
| FG9A | Patent granted |
Effective date: 20150625 |