KR970062024A - 에리스리톨의 제조방법 - Google Patents

에리스리톨의 제조방법 Download PDF

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KR970062024A
KR970062024A KR1019960002896A KR19960002896A KR970062024A KR 970062024 A KR970062024 A KR 970062024A KR 1019960002896 A KR1019960002896 A KR 1019960002896A KR 19960002896 A KR19960002896 A KR 19960002896A KR 970062024 A KR970062024 A KR 970062024A
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South Korea
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glucose
erythritol
kccm
days
liquid medium
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KR1019960002896A
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KR0162168B1 (ko
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박진병
육철
김정렬
박영근
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백운화
두산기술원 연구조합
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/145Fungal isolates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/645Fungi ; Processes using fungi

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
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  • Zoology (AREA)
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  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

트리코스포론속 미생물(기탁번호: KCCM-10077호)를 포도당 25~45%, 옥수수침지액 3~5%를 함유한 액체 배지에 정제함을 특징으로 하는 고수율 에리스리톨의 제조방법.

Description

에리스리톨의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (4)

  1. 트리코스포론속 미생물(기탁번호:KCCM-10077호)를 포도당25~45%, 옥수수침지액 3~5%를 함유한 액체 배지에 배양하고 정제함을 특징으로 하는 고수율 에리스리톨의 제조방법.
  2. 제1항에 있어서, 배양시 온도는 32~38℃이고, 공기주입속도는 0.8~1.2vvm, 교반조건은 200~500rpm임을 특징으로 하는 고수율 에리스리톨의 제조방법.
  3. 고수율로 에리스리톨을 생산하는 트리코스포론속 미생물(기탁번호:KCCM-10077호)
  4. 벌집통의 구성물을 35~45% 포도당과 1.0% 효모추출물로 구성된 액체배지에 현탁한 뒤 28~32℃에서 3~4일간 배양하고, 35~45% 포도당과 1.0% 효모추출물이 포함된 한천배지에 도말하여 28~32°C에서 3~4일간배양하여 생성된 균총을 각각 15~25% 포도당과 0.5% 효모추출몰, 유레아 0.1%로 구성된 액체 배지에서 4~6일간 현탁 배양하고, 박막크로마토그래피 및 HPLC를 이용하여 에리스리톨 생산 역가를 측정하고 우수 균주를 선발함을 특징으로 하는 제3항의 트리코스포론속 미생물(기탁번호:KCCM-10077호)의 분리방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019960002896A 1996-02-07 1996-02-07 에리스리톨의 제조방법 KR0162168B1 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019960002896A KR0162168B1 (ko) 1996-02-07 1996-02-07 에리스리톨의 제조방법

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019960002896A KR0162168B1 (ko) 1996-02-07 1996-02-07 에리스리톨의 제조방법

Publications (2)

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KR970062024A true KR970062024A (ko) 1997-09-12
KR0162168B1 KR0162168B1 (ko) 1998-11-16

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KR1019960002896A KR0162168B1 (ko) 1996-02-07 1996-02-07 에리스리톨의 제조방법

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KR0162168B1 (ko) 1998-11-16

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