KR950003821A - 가스측정용 바이오센서 및 그 제조방법 - Google Patents

가스측정용 바이오센서 및 그 제조방법 Download PDF

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KR950003821A
KR950003821A KR1019930013482A KR930013482A KR950003821A KR 950003821 A KR950003821 A KR 950003821A KR 1019930013482 A KR1019930013482 A KR 1019930013482A KR 930013482 A KR930013482 A KR 930013482A KR 950003821 A KR950003821 A KR 950003821A
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South Korea
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electrode
biosensor
enzyme
gas sensor
absorbent gel
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KR1019930013482A
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English (en)
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KR970001146B1 (ko
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박제균
이희진
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이헌조
주식회사 금성사
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Priority to KR1019930013482A priority Critical patent/KR970001146B1/ko
Priority to EP94630044A priority patent/EP0634488A3/en
Priority to JP6164426A priority patent/JP2838484B2/ja
Priority to US08/276,097 priority patent/US5656142A/en
Publication of KR950003821A publication Critical patent/KR950003821A/ko
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N25/00—Investigating or analyzing materials by the use of thermal means
    • G01N25/20—Investigating or analyzing materials by the use of thermal means by investigating the development of heat, i.e. calorimetry, e.g. by measuring specific heat, by measuring thermal conductivity
    • G01N25/22—Investigating or analyzing materials by the use of thermal means by investigating the development of heat, i.e. calorimetry, e.g. by measuring specific heat, by measuring thermal conductivity on combustion or catalytic oxidation, e.g. of components of gas mixtures
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/002—Electrode membranes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/817—Enzyme or microbe electrode

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
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  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Combustion & Propulsion (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

본 발명은 가스측정용 바이오센서에 관한 것으로, 기상의 유기화학물질을 측정할 수 있는 바이오센서로서 절연성기판(1)위에 전극부(2,3,4)와 상기 전극부의 연결패드(5) 및 절연층(6)을 구비하여 구성된 후막형 전기화학소자와, 상기 전기화학소자의 전극부상에 기상의 유기화학물질과 반응하는 효소가 고정화된 수분흡습성겔층(7)을 구비하여 이루어진 감응막으로 구성된 것을 제공한다.

Description

가스측정용 바이오센서 및 그 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 본 발명의 제1실시예에 의한 가스측정용 바이오센서 구조도, 제2도는 본 발명의 제2실시예에 의한 가스측정용 바이오센서 구조도, 제3도는 본 발명의 가스측정용 바이오센서의 특성을 설명하기 위한 그래프.

Claims (11)

  1. 절연성기판(1)위에 전극부(2,3,4)와 상기 전극부의 연결패드(5) 및 절연층(6)을 구비하여 구성된 후 막형 전기화학소자와, 상기 전기화학소자의 전극부상에 기상의 유기화학물질과 반응하는 효소가 고정화된 수분흡습성겔층(7)을 구비하여 이루어진 감응막으로 구성된 것을 특징으로 하는 가스측정용 바이오센서.
  2. 제1항에 있어서, 상기 전극부는 지시전극(2), 대전극(3), 비교전극(4)으로 구성됨을 특징으로 하는 가스측정용 바이오센서.
  3. 제1항에 있어서, 상기 전극부는 2개의 지시전극(2A,2B)과 비교전극(4)으로 구성됨을 특징으로 하는 가스측정용 바이오센서.
  4. 제1항 및 제3항에 있어서, 상기 전극부의 지시전극(2A)상에만 효소막(8)이 형성되고, 상기 전극부(2A,2B,4) 상부에 효소가 없는 수분흡습성겔층(9)이 더 구비됨을 특징으로 하는 가스측정용 바이오센서.
  5. 제1항에 있어서, 상기 고정화된 효소는 옥시도리덕테이즈 또는 하이드롤레이즈의 효소그롭으로 이루어진 것임을 특징으로 하느 가스측정용 바이오센서.
  6. 제5항에 있어서, 상기 효소그룹은 알코올 디하이드로게네이즈, 카본모녹사이드 디하이드로게네이즈, 훠메이트 디하이드로게네이즈, 알코올 옥시데이즈중의 어느 하나임을 특징으로 하는 가스측정용 바이오센서.
  7. 제1항에 있어서, 상기 수분흡수성겔층은 젤라틴, 폴리아크릴아마이드, 알지네이트, 아가로즈등의 어느 하나로 이루어진 것임을 특징으로 하는 가스측정용 바이오센서.
  8. 제7항에 있어서, 상기 수분흡수성겔층은 포티시움클로라이드가 포함된 것임을 특징으로 하는 가스측정용 바이오센서.
  9. 절연성기판(1)위에 전극부(2,3,4)와 전극부의 연결패드(5) 및 전연층(6)을 형성하여 후막형 전기화학소자를 제작하는 단계와, 수분흡슴성겔액에 효소액을 섞은 효소혼합액을 상기 후막형 전기화학소자의 전극부위에 떨어뜨린 후 건조시켜 수분흡습성겔층을 형성하는 단계로 이루어짐을 특징으로 하는 가스측정용 바이오센서의 제조방법.
  10. 제9항에 있어서, 상기 효소액은 완충용액에 효소 및 조효소를 녹여서 만드는 것을 특징으로 하는 가스측정용 바이오센서의 제조방법.
  11. 제9항에 있어서, 상기 수분흡습성겔액은 포티시움클로라이드용액에 젤라틴과 같은 수분흡습성겔을 녹여 만드는 것을 특징으로 하는 가스측정용 바이오센서의 제조방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019930013482A 1993-07-16 1993-07-16 가스측정용 바이오센서 및 그 제조방법 KR970001146B1 (ko)

Priority Applications (4)

Application Number Priority Date Filing Date Title
KR1019930013482A KR970001146B1 (ko) 1993-07-16 1993-07-16 가스측정용 바이오센서 및 그 제조방법
EP94630044A EP0634488A3 (en) 1993-07-16 1994-07-14 Biosensor for measuring a gas and method for its manufacture.
JP6164426A JP2838484B2 (ja) 1993-07-16 1994-07-15 ガス測定用バイオセンサー及びその製造方法
US08/276,097 US5656142A (en) 1993-07-16 1994-07-15 Biosensor for measuring gas and the manufacturing method thereof

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KR1019930013482A KR970001146B1 (ko) 1993-07-16 1993-07-16 가스측정용 바이오센서 및 그 제조방법

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KR970001146B1 KR970001146B1 (ko) 1997-01-29

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EP (1) EP0634488A3 (ko)
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Also Published As

Publication number Publication date
US5656142A (en) 1997-08-12
JPH0777511A (ja) 1995-03-20
EP0634488A2 (en) 1995-01-18
KR970001146B1 (ko) 1997-01-29
JP2838484B2 (ja) 1998-12-16
EP0634488A3 (en) 1995-05-03

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