KR950003445A - 양자리공의 항바이러스성 단백질(pap)을 발현하는 미생물 - Google Patents

양자리공의 항바이러스성 단백질(pap)을 발현하는 미생물 Download PDF

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KR950003445A
KR950003445A KR1019930012360A KR930012360A KR950003445A KR 950003445 A KR950003445 A KR 950003445A KR 1019930012360 A KR1019930012360 A KR 1019930012360A KR 930012360 A KR930012360 A KR 930012360A KR 950003445 A KR950003445 A KR 950003445A
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South Korea
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antiviral protein
pap
vector
microorganism
aries
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KR1019930012360A
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KR960015747B1 (ko
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문영호
전홍섭
최규환
이관호
김만근
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장기하
주식회사 진로
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Priority to KR1019930012360A priority Critical patent/KR960015747B1/ko
Priority to CA002102859A priority patent/CA2102859C/en
Priority to AU50642/93A priority patent/AU662844B2/en
Priority to EP93119118A priority patent/EP0637591A3/en
Priority to JP5337947A priority patent/JP2531930B2/ja
Priority to US08/342,786 priority patent/US5648234A/en
Publication of KR950003445A publication Critical patent/KR950003445A/ko
Application granted granted Critical
Publication of KR960015747B1 publication Critical patent/KR960015747B1/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/68—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment
    • A61K47/6801—Drug-antibody or immunoglobulin conjugates defined by the pharmacologically or therapeutically active agent
    • A61K47/6803—Drugs conjugated to an antibody or immunoglobulin, e.g. cisplatin-antibody conjugates
    • A61K47/6811—Drugs conjugated to an antibody or immunoglobulin, e.g. cisplatin-antibody conjugates the drug being a protein or peptide, e.g. transferrin or bleomycin
    • A61K47/6817—Toxins
    • A61K47/6819—Plant toxins
    • A61K47/6825—Ribosomal inhibitory proteins, i.e. RIP-I or RIP-II, e.g. Pap, gelonin or dianthin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A61P31/18—Antivirals for RNA viruses for HIV
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
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  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Botany (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Oncology (AREA)
  • Immunology (AREA)
  • Communicable Diseases (AREA)
  • Epidemiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • AIDS & HIV (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 미생물내에서 외래유전자의 발현을 가능하게 하는 벡터에 양자리공의 항바이러스성 단백질(PAP:Phytolacca antiviral protein)을 암호화하고 있는 유전자를 삽입하여 제조한 항바이러스성 단백질 유전자 발현벡터, 이 발현벡터를 미생물에 형질전환시킨 형질전환체 및 이 형질전환체를 배양함으로써 항바이러스성 단백질을 제조하는 방법에 관한 것이다.
본 발명의 벡터를 이용하여 항바이러스성 단백질을 생성하고 발현되는 형질전환 미생물을 제조함으로써, 생물학적 활성을 지닌 항바이러스성 단백질을 보다 용이하게 생산할 수 있음은 물론, AIDS등 치료에 사용되는 면역결합체 등의 제조에 사용할 수 있을 것이다.

Description

양자리공의 항바이러스성 단백질(PAP)을 발현하는 미생물
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제 1 도는 본 발명의 PAP를 발현하는 벡터 pMJ13를 제조하기 위한 전략을 나타내는 모식도이다. 제 2 도는 본 발명의 pMJ12를 나타내는 전기영동 사진이다. 제 3 도는 pMJ12로 형질전환된 대장균 HB101의 생장곡선을 나타낸 그래프이다.

Claims (5)

  1. 양자리공의 항바이러스성 단백질 (PAP: Phytolacca antiviral protein)유전자를 포함하는 재조합 벡터 pMJ12(KCCM 10037).
  2. 제 1 항의 재조합 벡터로 형절전환된 미생물.
  3. 제 1 항의 재조합 벡터로 형절전환된 대장균(E. coli) HB101(KCCM 10037).
  4. 제 1 항의 재조합 벡터로 형절전환된 대장균 HB101을 배양하여 분리, 정제함으로써, 양자리공의 재조합 항바이러스성 단백질을 제조하는 방법.
  5. 제 4 항의 방법에 의해 제조된 항바이러스성 단백질 재조합 PAP.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019930012360A 1993-07-02 1993-07-02 양자리공의 항바이러스성 단백질(pap)을 발현하는 미생물 KR960015747B1 (ko)

Priority Applications (6)

Application Number Priority Date Filing Date Title
KR1019930012360A KR960015747B1 (ko) 1993-07-02 1993-07-02 양자리공의 항바이러스성 단백질(pap)을 발현하는 미생물
CA002102859A CA2102859C (en) 1993-07-02 1993-11-10 Expression vector for phytolacca antiviral protein
AU50642/93A AU662844B2 (en) 1993-07-02 1993-11-11 A novel expression vector for phytolacca antiviral protein
EP93119118A EP0637591A3 (en) 1993-07-02 1993-11-26 New expression vector for the phytolacca antiviral protein.
JP5337947A JP2531930B2 (ja) 1993-07-02 1993-12-28 アメリカヤマゴボウの抗ウィルス性タンパク質を発現する微生物
US08/342,786 US5648234A (en) 1993-07-02 1994-11-21 Expression vector for Phytolacca antiviral protein

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019930012360A KR960015747B1 (ko) 1993-07-02 1993-07-02 양자리공의 항바이러스성 단백질(pap)을 발현하는 미생물

Publications (2)

Publication Number Publication Date
KR950003445A true KR950003445A (ko) 1995-02-16
KR960015747B1 KR960015747B1 (ko) 1996-11-20

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Application Number Title Priority Date Filing Date
KR1019930012360A KR960015747B1 (ko) 1993-07-02 1993-07-02 양자리공의 항바이러스성 단백질(pap)을 발현하는 미생물

Country Status (6)

Country Link
US (1) US5648234A (ko)
EP (1) EP0637591A3 (ko)
JP (1) JP2531930B2 (ko)
KR (1) KR960015747B1 (ko)
AU (1) AU662844B2 (ko)
CA (1) CA2102859C (ko)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110016470A (zh) * 2019-05-09 2019-07-16 江苏森瑞谱生物制药有限公司 一种从商陆中提取纯化商陆抗病毒蛋白的方法

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR970001565B1 (ko) * 1993-08-28 1997-02-11 장기하 섬자리공 항바이러스성 단백질(pip) 유전자 및 이를 발현하는 미생물
KR970007864B1 (ko) * 1994-07-21 1997-05-17 진로 주식회사 섬자리공의 항바이러스성 단백질(pip)을 발현하는 식물체의 제조방법
KR970074934A (ko) * 1996-05-22 1997-12-10 문상목 섬자리공 항바이러스성 단백질의 신규한 유전자 및 그를 발현하는 재조합 미생물

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57106625A (en) * 1980-12-22 1982-07-02 Teijin Ltd Cytotoxic protein complex and its preparation
US5101025A (en) * 1989-04-04 1992-03-31 Genelabs Incorporated Recombinant trichosanthin and coding sequence
DE69131253T2 (de) * 1990-10-09 2000-02-24 New York University New York Zur behandlung von tumoren und hiv-infektionen nützliches pflanzliches protein
US5376546A (en) * 1991-11-04 1994-12-27 Xoma Corporation Analogs of ribosome-inactivating proteins
KR970001565B1 (ko) * 1993-08-28 1997-02-11 장기하 섬자리공 항바이러스성 단백질(pip) 유전자 및 이를 발현하는 미생물

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110016470A (zh) * 2019-05-09 2019-07-16 江苏森瑞谱生物制药有限公司 一种从商陆中提取纯化商陆抗病毒蛋白的方法

Also Published As

Publication number Publication date
AU662844B2 (en) 1995-09-14
AU5064293A (en) 1995-01-19
KR960015747B1 (ko) 1996-11-20
EP0637591A3 (en) 1995-05-24
CA2102859C (en) 1998-04-28
JP2531930B2 (ja) 1996-09-04
EP0637591A2 (en) 1995-02-08
US5648234A (en) 1997-07-15
JPH0767660A (ja) 1995-03-14
CA2102859A1 (en) 1995-01-03

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