KR940005668A - 성장 호르몬 방출 호르몬 수용체의 분리 및 특성화 - Google Patents
성장 호르몬 방출 호르몬 수용체의 분리 및 특성화 Download PDFInfo
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- KR940005668A KR940005668A KR1019930011572A KR930011572A KR940005668A KR 940005668 A KR940005668 A KR 940005668A KR 1019930011572 A KR1019930011572 A KR 1019930011572A KR 930011572 A KR930011572 A KR 930011572A KR 940005668 A KR940005668 A KR 940005668A
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- growth hormone
- releasing hormone
- hormone releasing
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- 101710142969 Somatoliberin Proteins 0.000 title claims description 17
- 108020003175 receptors Proteins 0.000 title claims description 4
- 239000000095 Growth Hormone-Releasing Hormone Substances 0.000 title claims 13
- 102000038461 Growth Hormone-Releasing Hormone Human genes 0.000 title 1
- 238000012512 characterization method Methods 0.000 title 1
- 238000002955 isolation Methods 0.000 title 1
- 101710198286 Growth hormone-releasing hormone receptor Proteins 0.000 claims abstract 15
- 102100033365 Growth hormone-releasing hormone receptor Human genes 0.000 claims abstract 15
- 238000000034 method Methods 0.000 claims abstract 11
- 102100022831 Somatoliberin Human genes 0.000 claims description 16
- FUOJEDZPVVDXHI-UHFFFAOYSA-N (2,5-dioxopyrrolidin-1-yl) 5-azido-2-nitrobenzoate Chemical compound [O-][N+](=O)C1=CC=C(N=[N+]=[N-])C=C1C(=O)ON1C(=O)CCC1=O FUOJEDZPVVDXHI-UHFFFAOYSA-N 0.000 claims description 3
- 238000004132 cross linking Methods 0.000 claims description 3
- 230000001817 pituitary effect Effects 0.000 claims description 2
- XMBWDFGMSWQBCA-UHFFFAOYSA-N hydrogen iodide Chemical compound I XMBWDFGMSWQBCA-UHFFFAOYSA-N 0.000 claims 4
- 238000001042 affinity chromatography Methods 0.000 claims 3
- 108010071586 (N-acetyl-tyr1,D-arg2)fragment 1-29 amide Proteins 0.000 claims 2
- 230000008878 coupling Effects 0.000 claims 2
- 238000010168 coupling process Methods 0.000 claims 2
- 238000005859 coupling reaction Methods 0.000 claims 2
- 230000005855 radiation Effects 0.000 claims 2
- 102000005962 receptors Human genes 0.000 claims 2
- -1 sulfosuccinimidyl Chemical group 0.000 claims 2
- NGXDNMNOQDVTRL-UHFFFAOYSA-N (2,5-dioxopyrrolidin-1-yl) 6-(4-azido-2-nitroanilino)hexanoate Chemical compound [O-][N+](=O)C1=CC(N=[N+]=[N-])=CC=C1NCCCCCC(=O)ON1C(=O)CCC1=O NGXDNMNOQDVTRL-UHFFFAOYSA-N 0.000 claims 1
- 102000018997 Growth Hormone Human genes 0.000 claims 1
- 108010051696 Growth Hormone Proteins 0.000 claims 1
- 108010090804 Streptavidin Proteins 0.000 claims 1
- 239000007801 affinity label Substances 0.000 claims 1
- 210000004198 anterior pituitary gland Anatomy 0.000 claims 1
- 239000003242 anti bacterial agent Substances 0.000 claims 1
- 229940088710 antibiotic agent Drugs 0.000 claims 1
- 230000003115 biocidal effect Effects 0.000 claims 1
- 238000004587 chromatography analysis Methods 0.000 claims 1
- 239000000122 growth hormone Substances 0.000 claims 1
- FUZZWVXGSFPDMH-UHFFFAOYSA-N hexanoic acid Chemical compound CCCCCC(O)=O FUZZWVXGSFPDMH-UHFFFAOYSA-N 0.000 claims 1
- 238000004128 high performance liquid chromatography Methods 0.000 claims 1
- 230000003100 immobilizing effect Effects 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 230000003287 optical effect Effects 0.000 claims 1
- 239000007856 photoaffinity label Substances 0.000 claims 1
- 239000000758 substrate Substances 0.000 claims 1
- 239000008188 pellet Substances 0.000 abstract description 3
- 239000012528 membrane Substances 0.000 abstract description 2
- 239000003446 ligand Substances 0.000 abstract 7
- 230000009870 specific binding Effects 0.000 abstract 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000022811 deglycosylation Effects 0.000 description 1
Classifications
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- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01L—SEMICONDUCTOR DEVICES NOT COVERED BY CLASS H10
- H01L27/00—Devices consisting of a plurality of semiconductor or other solid-state components formed in or on a common substrate
- H01L27/02—Devices consisting of a plurality of semiconductor or other solid-state components formed in or on a common substrate including semiconductor components specially adapted for rectifying, oscillating, amplifying or switching and having potential barriers; including integrated passive circuit elements having potential barriers
- H01L27/04—Devices consisting of a plurality of semiconductor or other solid-state components formed in or on a common substrate including semiconductor components specially adapted for rectifying, oscillating, amplifying or switching and having potential barriers; including integrated passive circuit elements having potential barriers the substrate being a semiconductor body
- H01L27/10—Devices consisting of a plurality of semiconductor or other solid-state components formed in or on a common substrate including semiconductor components specially adapted for rectifying, oscillating, amplifying or switching and having potential barriers; including integrated passive circuit elements having potential barriers the substrate being a semiconductor body including a plurality of individual components in a repetitive configuration
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/60—Growth hormone-releasing factor [GH-RF], i.e. somatoliberin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/72—Receptors; Cell surface antigens; Cell surface determinants for hormones
- C07K14/723—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH receptor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/575—Hormones
- G01N2333/60—Growth-hormone releasing factors (GH-RF) (Somatoliberin)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
- G01N2333/72—Assays involving receptors, cell surface antigens or cell surface determinants for hormones
- G01N2333/726—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH
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- Health & Medical Sciences (AREA)
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- Proteomics, Peptides & Aminoacids (AREA)
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- Cell Biology (AREA)
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- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
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Abstract
요오드화 GHRH 표지자를 사용하는 독특한 결합 검정법을 사용하여 성장 호르몬 방출 호르몬(GHRH)수용체 결합을 특성화했다. 광친화성 GHRH 표시자는 수용체의 광라벨링 및 특성화를 위해 구성했다.
게다가, 고 친화성 비오틴화 GHRH 동족체를 구성했다. GHRH-R/GHRH 복합체의 용도 및 온화한 세정액, 이어서 친화 크로마토그래피를 사용하는 특이적으로 결합된 GHRH 의 추출은 실질적으로 정제된 GHRH-R 분리물을 산출했다. GHRH- R 분리물의 전기영동 처리는 수용체의 서열화를 수행하기에 충분한 순도의 GHRH-R 을 생성한다.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 조(組)양의 뇌하수체 막펠렛에 대한1251-GHRHa의 결합을 나타내는 막대그래프이다.
제2도는 포화 결합 분석을 위해 사용된 그래프이다.
제3도는 수용체의 광친화성 가교를 나타내는 SDS겔의 방사선 사진이다.
제4도는 10nM GHRH 에 의한 경쟁을 나타내며 또한 탈글리코 실화를 보이는 CHAPS 추출 ANB-NOS 가교를 보이는 제3도에서와 같은 SDS 겔의 방사선 사진이다.
Claims (21)
- 서열화하기에 충분한 순도의 성장 호르몬 방출 호르몬 수용체.
- 성장 호르몬 방출 호르몬 수용체 분리물.
- 제2항에 있어서, 성장 호르몬 방출 호르몬 수용체를 서열화 하기에 충분한 순도인 분리물.
- 복합 성장 호르몬 방출 수용체를 친화 크로마토그래피 지지체 상에 고정시킨후 상기 지지체상의 상기 복합체를 해리시켜 성장 호르몬 방출 호르몬 수용체 분리물을 생성하는 단계들로 구성되는 성장 호르몬 방출 호르몬 수용체 분리를 생성방법.
- 제4항에 있어서, 상기 성장 호르몬 방출 홀몬 수용체는 성장 호르몬 방출 호르몬 또는 성장 호르몬 방출호르몬 동족체화 복합되며, 부가적으로 상기 복합체를 상기 지지체에 고정하기 전에, 상기 복합체를 온화한 세정액에 용해시키고, pH를 낮추므로써 상기 지지체상에 고정된 복합체를 해리시키는 단계들을 포함하는 방법.
- 제5항에 있어서, 상기 수용체는 성장 호르몬 방출 호르몬 동족체에 결합되고, 상기 동족체는 친화 크로마토그래피 지지체에 결합할 수 있는 관능을 갖는 방법.
- 제6항에 있어서, 상기 동족체는 비오친화되고 상기 크로마토 그래피 지지체는 상기 비오틴화 동족체에 결합할 수 있는 스트렙타비딘 관능가를 함유하는 방법.
- 성장 호르몬 방출 호르몬 수용체에 결합할 수 있고 친화 크로마토그래피 지지체에 결합할 수 있는 성장호르몬 방출 호르몬 동족체.
- 제8항에 있어서, [His1, Nie27, 비오티닐-Lys41]-GHRH-(1-4)-NH2인 동족체.
- UV 방사선 부재하에 성장 호르몬 방출 호르몬 수용에 결합할 수 있고 UV 방사선 존재하에 성장 호르몬 방출 호르몬 수용체에 가교 할 수 있는 성장 호르몬 방출 호르몬 동족체.
- 제10항에 있어서, GHRHa-SANPAH로서, 이때 GHRHa가 [His1, NIe27]-GHRH-(1-32)-NH2이고, SANPAH가 설포숙신이미딜 6-(4,-아지도-2,니트로페닐아미노)헥사노에이트인 동족체.
- 제10항에 있어서,GHRHa가 [His1,NIe27]-GHRH-(1-32)-NH2이고 ANB-NOS 가 N-S-아지도-2-니트로벤조일옥시숙신이미드인 동족체.
- N-5-아지도-2-니트로벤조일옥시숙신이미드와 GHRH 동족체 [His1, NIe27]-GHRH-(1-32)-NH2의 커플링 단계로 구성되는 광-친화성 표지가 생성방법.
- 설포숙신이미딜6-(4´-아지도-2´-니트로페닐 암모니오)헥사노 에이트를 GHRH동족체[His1,NIe27]-GHRH-(1-32)-NH2에 커플링시키는 광-친화성 표지가 생성 방법.
- 세공-형성 항생제의 존재하에 조 뇌하수체 전엽 조직과 GHRH의 접촉 단계로 구성되는 조 뇌하수체 전엽조직에 대한 성장 호르몬 방출 호르몬의 결합 방법.
- 제5항에 있어서, 상기 항생제가 약 0.05 ㎎/㎖로 존재하는 아라메티신인 방법.
- 성장 호르몬 방출 호르몬 또는 성장 호르몬 방출 호르몬 동족체를, 상기 성장 호르몬 방출 호르몬 또는 성장 호르몬 방출 호르몬 동족체와 요오드화 하기에 충분한 요오드화 기질의 접촉 단계로 구성되는 성장 호르몬 방출 호르몬 수용체 표지가 생성 방법.
- 제17항에 있어서, 상기 요오드화 성장 호르몬 방출 호르몬 또는 성장 호르몬 방출 호르몬 동족체를 HPLC를 거쳐 상기 요오드화 후에 정제하는 방법.
- 제4항의 방법에 의해 생성된 성장 호르몬 방출 호르몬 수용체 분리물.
- 제7항의 방법에 의해 생산된 성장 호르몬 방출 호르몬 수용체 분리물.
- 자연적으로 발견되는 것보다 큰 농도로 존재하는 성장 호르몬 방출 호르몬 수용체를 함유하는 조성물.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US07/902,826 | 1992-06-23 | ||
| US7/902,826 | 1992-06-23 | ||
| US07/902,826 US5973114A (en) | 1992-06-23 | 1992-06-23 | Isolation and characterization of the growth hormone releasing hormone receptor |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR940005668A true KR940005668A (ko) | 1994-03-22 |
| KR100329729B1 KR100329729B1 (ko) | 2002-07-27 |
Family
ID=25416459
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1019930011572A KR100329729B1 (ko) | 1992-06-23 | 1993-06-22 | 성장호르몬방출호르몬수용체의분리및특성화 |
| KR1019930011573A KR940001411A (ko) | 1992-06-23 | 1993-06-24 | 예정된 메모리 용량을 가진 집적 반도체 메모리의 제조방법 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1019930011573A KR940001411A (ko) | 1992-06-23 | 1993-06-24 | 예정된 메모리 용량을 가진 집적 반도체 메모리의 제조방법 |
Country Status (16)
| Country | Link |
|---|---|
| US (1) | US5973114A (ko) |
| EP (2) | EP0576988B1 (ko) |
| JP (2) | JP3410094B2 (ko) |
| KR (2) | KR100329729B1 (ko) |
| AT (2) | ATE205222T1 (ko) |
| AU (1) | AU672975B2 (ko) |
| CA (1) | CA2098972C (ko) |
| DE (2) | DE69334264D1 (ko) |
| DK (2) | DK0576988T3 (ko) |
| ES (2) | ES2164648T3 (ko) |
| GR (1) | GR3036766T3 (ko) |
| IL (1) | IL106112A (ko) |
| NZ (2) | NZ272761A (ko) |
| PT (2) | PT1104888E (ko) |
| WO (1) | WO1994000482A1 (ko) |
| ZA (1) | ZA934483B (ko) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5852187A (en) * | 1992-06-23 | 1998-12-22 | The University Of Virginia Patent Foundation | Molecular cloning of the ovine pituitary growth hormone releasing hormone receptor |
| IL106860A (en) * | 1992-09-15 | 1999-08-17 | Thorner Michael O | Entertainment, characterization and protein sequencing of a hormone receptor that releases growth hormone and replication of the encoded gene for a hormone that releases growth hormone |
| US5967795A (en) * | 1995-08-30 | 1999-10-19 | Asea Brown Boveri Ab | SiC semiconductor device comprising a pn junction with a voltage absorbing edge |
| US6221617B1 (en) | 1996-12-06 | 2001-04-24 | Julie Heinrich | Bioassay for growth hormone releasing hormone |
| WO2000076455A2 (en) * | 1999-06-12 | 2000-12-21 | University Of Virginia Patent Foundation | Chicken growth hormone releasing hormone receptor |
| US7078514B1 (en) * | 1999-06-12 | 2006-07-18 | Michael O. Thorner | Chicken growth hormone releasing hormone receptor |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4833166A (en) * | 1987-05-01 | 1989-05-23 | Grosvenor Clark E | Growth hormone releasing hormone complementary peptides |
| CA2040649A1 (en) * | 1990-04-24 | 1991-10-25 | Gerald S. Brooke | Growth hormone releasing factor analogs |
| CA2085362A1 (en) * | 1990-06-29 | 1991-12-30 | Arthur M. Felix | Histidine substituted growth hormone releasing factor analogs |
| US5225543A (en) * | 1991-03-28 | 1993-07-06 | American Cyanamid Company | Receptors method for purification of g protein-linked |
| IL106860A (en) * | 1992-09-15 | 1999-08-17 | Thorner Michael O | Entertainment, characterization and protein sequencing of a hormone receptor that releases growth hormone and replication of the encoded gene for a hormone that releases growth hormone |
-
1992
- 1992-06-23 US US07/902,826 patent/US5973114A/en not_active Expired - Lifetime
-
1993
- 1993-06-21 NZ NZ272761A patent/NZ272761A/en not_active IP Right Cessation
- 1993-06-21 NZ NZ247941A patent/NZ247941A/en not_active IP Right Cessation
- 1993-06-22 KR KR1019930011572A patent/KR100329729B1/ko not_active IP Right Cessation
- 1993-06-22 CA CA002098972A patent/CA2098972C/en not_active Expired - Lifetime
- 1993-06-22 ZA ZA934483A patent/ZA934483B/xx unknown
- 1993-06-23 PT PT01105156T patent/PT1104888E/pt unknown
- 1993-06-23 DE DE69334264T patent/DE69334264D1/de not_active Expired - Lifetime
- 1993-06-23 EP EP93110030A patent/EP0576988B1/en not_active Expired - Lifetime
- 1993-06-23 DK DK93110030T patent/DK0576988T3/da active
- 1993-06-23 DE DE69330695T patent/DE69330695T2/de not_active Expired - Lifetime
- 1993-06-23 PT PT93110030T patent/PT576988E/pt unknown
- 1993-06-23 DK DK01105156T patent/DK1104888T3/da active
- 1993-06-23 ES ES93110030T patent/ES2164648T3/es not_active Expired - Lifetime
- 1993-06-23 WO PCT/US1993/005870 patent/WO1994000482A1/en unknown
- 1993-06-23 AU AU41467/93A patent/AU672975B2/en not_active Expired
- 1993-06-23 ES ES01105156T patent/ES2323218T3/es not_active Expired - Lifetime
- 1993-06-23 IL IL10611293A patent/IL106112A/en not_active IP Right Cessation
- 1993-06-23 AT AT93110030T patent/ATE205222T1/de active
- 1993-06-23 JP JP50248194A patent/JP3410094B2/ja not_active Expired - Lifetime
- 1993-06-23 EP EP01105156A patent/EP1104888B1/en not_active Expired - Lifetime
- 1993-06-23 AT AT01105156T patent/ATE424563T1/de active
- 1993-06-24 KR KR1019930011573A patent/KR940001411A/ko not_active Application Discontinuation
-
2001
- 2001-10-01 GR GR20010401623T patent/GR3036766T3/el unknown
-
2002
- 2002-07-23 JP JP2002213966A patent/JP3456989B2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| DE69330695T2 (de) | 2002-07-04 |
| JP3456989B2 (ja) | 2003-10-14 |
| DE69334264D1 (de) | 2009-04-16 |
| GR3036766T3 (en) | 2002-01-31 |
| JP3410094B2 (ja) | 2003-05-26 |
| DE69330695D1 (de) | 2001-10-11 |
| ES2323218T3 (es) | 2009-07-09 |
| JP2003048898A (ja) | 2003-02-21 |
| JPH07500614A (ja) | 1995-01-19 |
| KR940001411A (ko) | 1994-01-11 |
| DK0576988T3 (da) | 2001-11-12 |
| ATE205222T1 (de) | 2001-09-15 |
| EP0576988A1 (en) | 1994-01-05 |
| NZ247941A (en) | 1997-04-24 |
| PT576988E (pt) | 2002-01-30 |
| IL106112A (en) | 1998-12-06 |
| CA2098972A1 (en) | 1993-12-24 |
| WO1994000482A1 (en) | 1994-01-06 |
| ZA934483B (en) | 1994-04-18 |
| EP1104888A2 (en) | 2001-06-06 |
| EP1104888A3 (en) | 2004-03-03 |
| PT1104888E (pt) | 2009-05-14 |
| US5973114A (en) | 1999-10-26 |
| AU672975B2 (en) | 1996-10-24 |
| ES2164648T3 (es) | 2002-03-01 |
| ATE424563T1 (de) | 2009-03-15 |
| KR100329729B1 (ko) | 2002-07-27 |
| CA2098972C (en) | 2003-08-19 |
| DK1104888T3 (da) | 2009-05-25 |
| AU4146793A (en) | 1994-01-06 |
| NZ272761A (en) | 1997-04-24 |
| IL106112A0 (en) | 1993-10-20 |
| EP1104888B1 (en) | 2009-03-04 |
| EP0576988B1 (en) | 2001-09-05 |
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