ATE173831T1 - Methode zur bildung eines aminosäurehydantoinderivats unter verwendung eines n-substituierten ketenimins als aktivator - Google Patents

Methode zur bildung eines aminosäurehydantoinderivats unter verwendung eines n-substituierten ketenimins als aktivator

Info

Publication number
ATE173831T1
ATE173831T1 AT91913907T AT91913907T ATE173831T1 AT E173831 T1 ATE173831 T1 AT E173831T1 AT 91913907 T AT91913907 T AT 91913907T AT 91913907 T AT91913907 T AT 91913907T AT E173831 T1 ATE173831 T1 AT E173831T1
Authority
AT
Austria
Prior art keywords
amino acid
ketenimine
substituted
forming
hydantoine
Prior art date
Application number
AT91913907T
Other languages
English (en)
Inventor
Victoria Boyd
David Hawke
Timothy G Geiser
Original Assignee
Perkin Elmer Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Perkin Elmer Corp filed Critical Perkin Elmer Corp
Application granted granted Critical
Publication of ATE173831T1 publication Critical patent/ATE173831T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6818—Sequencing of polypeptides
    • G01N33/6821—Sequencing of polypeptides involving C-terminal degradation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/81—Carrier - bound or immobilized peptides or proteins and the preparation thereof, e.g. biological cell or cell fragment as carrier
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25125—Digestion or removing interfering materials
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
    • Y10T436/255—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.] including use of a solid sorbent, semipermeable membrane, or liquid extraction

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Low-Molecular Organic Synthesis Reactions Using Catalysts (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
AT91913907T 1990-06-29 1991-06-21 Methode zur bildung eines aminosäurehydantoinderivats unter verwendung eines n-substituierten ketenimins als aktivator ATE173831T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/546,303 US5051368A (en) 1990-06-29 1990-06-29 Method for forming an amino acid thiohydantoin using an N-substituted ketenimine activator

Publications (1)

Publication Number Publication Date
ATE173831T1 true ATE173831T1 (de) 1998-12-15

Family

ID=24179804

Family Applications (1)

Application Number Title Priority Date Filing Date
AT91913907T ATE173831T1 (de) 1990-06-29 1991-06-21 Methode zur bildung eines aminosäurehydantoinderivats unter verwendung eines n-substituierten ketenimins als aktivator

Country Status (6)

Country Link
US (1) US5051368A (de)
EP (1) EP0536324B1 (de)
JP (1) JP2989007B2 (de)
AT (1) ATE173831T1 (de)
DE (1) DE69130530T2 (de)
WO (1) WO1992000284A2 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5304497A (en) * 1991-10-15 1994-04-19 Applied Biosystems, Inc. Method of forming N-protected amino acid thiohydantoins
US5185266A (en) * 1991-10-15 1993-02-09 Applied Biosystems, Inc. Cleavage method for acyl thiohydantoins and use of the method in c-terminal peptide sequencing
US5227309A (en) * 1991-10-18 1993-07-13 City Of Hope C-terminal peptide sequencing method
US5602207A (en) * 1993-01-11 1997-02-11 The Perkin-Elmer Corporation Support and method for immobilizing polypeptides
US5665603A (en) * 1993-07-26 1997-09-09 The Perkin-Elmer Corporation Thiohydantoin formation and selective modification of the carboxy terminus of an aspartic acid- and/or glutamic acid-containing protein
US5468843A (en) * 1994-09-08 1995-11-21 Perkin-Elmer Acetic anhydride activation for C-terminal protein sequencing
JP3124507B2 (ja) * 1996-05-24 2001-01-15 セイコーインスツルメンツ株式会社 タンパク質あるいはペプチドのカルボキシ末端からのアミノ酸配列を決定する方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4289853A (en) * 1977-10-03 1981-09-15 Illinois Water Treatment Company High loading of immobilized enzymes on activated carbon supports
CA1292840C (en) * 1985-09-26 1991-12-03 David Harry Hawke Sequencing of peptides
US4935494A (en) * 1988-11-15 1990-06-19 City Of Hope C-terminal peptide and protein sequencing

Also Published As

Publication number Publication date
US5051368A (en) 1991-09-24
DE69130530T2 (de) 1999-06-10
DE69130530D1 (de) 1999-01-07
EP0536324B1 (de) 1998-11-25
JPH05508413A (ja) 1993-11-25
WO1992000284A3 (en) 1992-04-30
WO1992000284A2 (en) 1992-01-09
EP0536324A1 (de) 1993-04-14
JP2989007B2 (ja) 1999-12-13

Similar Documents

Publication Publication Date Title
Fleer et al. The primary structure of bovine pancreatic phospholipase A2
De Haas et al. Studies on phospholipase A and its zymogen from porcine pancreas: I. The complete amino acid sequence
ATE109513T1 (de) Verfahren zur reinigung von rekombinanten proteinen und die verwendung von dessen produkten.
LU91013I2 (fr) ReFacto (Moroctocog alfa) dans toutes ses formes couvertes par le brevet de base
DK60586A (da) Inhibin isoleret fra ovariefollikelvaeske og fremgangsmaade til isolering og rensning af inhibin
PL374311A1 (en) Methods for purifying protein
JPS62106100A (ja) タンパク質アミノ末端残基の選択的化学的除去法
ATE317912T1 (de) Verfahren zur herstellung von beta-lactamen
ES2079621T3 (es) Procedimiento de identificacion o de titulacion de proteinas y aplicaciones.
ATE173831T1 (de) Methode zur bildung eines aminosäurehydantoinderivats unter verwendung eines n-substituierten ketenimins als aktivator
US5082926A (en) Polypeptide with type iv collagen cell adhesion, spreading and motility activity
JPS62171699A (ja) 蛋白質の製造法
TAKAGI et al. Amino acid sequence of troponin C obtained from ascidian (Halocynthia roretzi) body wall muscle
DE69711073D1 (de) Verfahren zur erkennung von lebenden mikrobiologischen verunreinigungen in einer lebensmittelprobe
DE69534263D1 (de) Expression kassette eines proteines p30 aus toxoplasma gondii
ATE370412T1 (de) Verfahren und verwendung eines polypeptids in der sperma-eizellen-bindung zur erhöhung oder verringerung der fertilität
MERRILL et al. Amino acid sequence of UP1, an hnRNP‐derived single‐stranded nucleic acid binding protein from calf thymus
DE68904578T2 (de) Enzym zur alpha-amidierung von c-termini und verfahren zu dessen herstellung.
Needleman et al. Sequencing peptides and proteins lacking free α-amino groups
Best et al. Studies on glycoproteins XII. About glycosidic-ester linkages in submaxillary gland glycoproteins prepared from sheep
Wang et al. Modification of aspartic acid residues to induce trypsin cleavage
EP1621546B1 (de) Peptidische Ligande die eine spezifische Bindungsfähigkeit für Immunoglobuline zeigen
DE58908128D1 (de) Verbindungen zur Einführung einer SH-Gruppe in Tyrosin.
Roffman et al. The extent of oxidative mitogenesis does not correlate with the degree of aldehyde formation of the T lymphocyte membrane.
DE3827416A1 (de) Peptide mit prourokinase-sequenzen, verfahren zu ihrer herstellung und ihre verwendung, antikoerper gegen diese peptide und mittel enthaltend diese antikoerper zur bestimmung von prourokinase

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties