KR940004056A - 항바이러스성 단백질 발현벡터와 이것으로 형질전환된 식물체의 제조방법 - Google Patents

항바이러스성 단백질 발현벡터와 이것으로 형질전환된 식물체의 제조방법 Download PDF

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KR940004056A
KR940004056A KR1019920014895A KR920014895A KR940004056A KR 940004056 A KR940004056 A KR 940004056A KR 1019920014895 A KR1019920014895 A KR 1019920014895A KR 920014895 A KR920014895 A KR 920014895A KR 940004056 A KR940004056 A KR 940004056A
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antiviral protein
plant
expression vector
present
protein
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KR1019920014895A
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KR950012900B1 (ko
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김만근
이관호
나병국
정한승
최규환
문영호
전홍섭
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장기하
주식회사 진로
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Priority to KR1019920014895A priority Critical patent/KR950012900B1/ko
Priority to JP5128222A priority patent/JP2522151B2/ja
Priority to DE69306067T priority patent/DE69306067T2/de
Priority to EP93110445A priority patent/EP0585554B1/en
Publication of KR940004056A publication Critical patent/KR940004056A/ko
Priority to US08/373,858 priority patent/US5633155A/en
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Publication of KR950012900B1 publication Critical patent/KR950012900B1/ko

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
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  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
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  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

본 발명은 식물세포내에서 외래유전자의 발현을 가능하게 하는 벡터에 양자리공의 cDNA 라이브러리에서 분리한 항바이러스성 단백질을 암호화하는 유전자를 삽입하여 제조한 항바이러스성 단백질 유전자 발현벡터를 식물세포에 형질전환시키고 배양함으로써 항바이러스성 단백질을 생성하는 식물체를 제조하는 방법에 관한 것이다. 본 발명은 또한 상기 방법에 사용되는 유전자를 포함하는 제조합백터의 제조에 관한 것이다. 본 발명의 방법을 이용하여 항바이러스성 단백질을 생성하고 발현되는 형질전환 식물체를 제조함으로써, 여러 종류의 바이러스에 대하여 내성이 강한 식물체를 개발할 수 있으며, 아울려 형질전환 생물체에서 항바이러스성 단백질을 제조할 수 있을 것이다.

Description

항바이러스성 단백질 발현벡터와 이것으로 형질전환된 식물체의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제 1 도는 cDNA 라이브러리로부터 순수분리한 PAP 유전자의 완전한 염기서열을 나타내고,
제 2 도는 발현벡터 pJRM100을 제조하는 일련의 순서를 도식화한 그림이며,
제 3 도는 발현벡터 pJRM100을 제한효소로 처리하여 아가로스 겔 전기영동한 결과를 나타내는 사진이며,
제 4 도는 발현벡터 pJRM100을 이용한 담배세포의 형질전환 과정을 순서대로 보여주고 있으며,

Claims (6)

  1. 항바이러스성 단백질 유전자를 식물체에서 발현시키는 재조합 유전자 pJRM100(KCTC 0052BP).
  2. 제 1 항의 재조합 유전자로 형질전환된 아그로박테리움 투메파시엔스(Agrobacterium tumefaciens LBA 4404).
  3. 제 1 항의 재조합 유전자로 형질전환된 아그로박테리움을 매개로 형질전환된 식물세포를 배양함으로써 항바이러스성 단백질을 생성하는 식물체를 제조하는 방법.
  4. 제 3 항에 있어서, 항바이러스성 단백질은 PAP(phytolacca anti-viral protein)인 방법.
  5. 제 3 항에 있어서, 식물체는 담배인 방법.
  6. 제 3 항의 방법에 의해 제조된 식물체로부터 생산되는 항바이러스성 단백질 PAP(phytolacca anti-vira1 protein) .
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019920014895A 1992-08-16 1992-08-19 항바이러스성 단백질 발현벡터와 이것으로 형질전환된 식물체의 제조방법 KR950012900B1 (ko)

Priority Applications (5)

Application Number Priority Date Filing Date Title
KR1019920014895A KR950012900B1 (ko) 1992-08-19 1992-08-19 항바이러스성 단백질 발현벡터와 이것으로 형질전환된 식물체의 제조방법
JP5128222A JP2522151B2 (ja) 1992-08-19 1993-04-30 抗ウィルス性タンパク質発現ベクタ―およびそれにより形質転換された植物体の製造方法
DE69306067T DE69306067T2 (de) 1992-08-19 1993-06-30 Verfahren zur Herstellung einer transgenen Pflanze, die das antivirale Protein aus Phytolacca exprimiert
EP93110445A EP0585554B1 (en) 1992-08-16 1993-06-30 Process for preparing a transgenic plant expressing phytolacca antiviral protein
US08/373,858 US5633155A (en) 1992-08-19 1995-01-18 Expression vector for phytolacca antiviral protein and process for preparing transgenic plant transformed therewith

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019920014895A KR950012900B1 (ko) 1992-08-19 1992-08-19 항바이러스성 단백질 발현벡터와 이것으로 형질전환된 식물체의 제조방법

Publications (2)

Publication Number Publication Date
KR940004056A true KR940004056A (ko) 1994-03-14
KR950012900B1 KR950012900B1 (ko) 1995-10-23

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KR1019920014895A KR950012900B1 (ko) 1992-08-16 1992-08-19 항바이러스성 단백질 발현벡터와 이것으로 형질전환된 식물체의 제조방법

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EP (1) EP0585554B1 (ko)
JP (1) JP2522151B2 (ko)
KR (1) KR950012900B1 (ko)
DE (1) DE69306067T2 (ko)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6757163B2 (en) 2001-11-19 2004-06-29 Samsung Electronics Co., Ltd. Computer having an auxiliary memory unit removably fastened on a fixing bracket

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6608241B1 (en) * 1985-10-29 2003-08-19 Monsanto Technology Llc Protection of plants against viral infection
KR970007864B1 (ko) * 1994-07-21 1997-05-17 진로 주식회사 섬자리공의 항바이러스성 단백질(pip)을 발현하는 식물체의 제조방법
KR970074934A (ko) * 1996-05-22 1997-12-10 문상목 섬자리공 항바이러스성 단백질의 신규한 유전자 및 그를 발현하는 재조합 미생물

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4921841A (en) * 1982-12-14 1990-05-01 Obrig Thomas G Immunoaffinity purification of phytolaccin proteins and their use in treating Herpes Simplex virus type II
KR910014790A (ko) * 1990-01-10 1991-08-31 한태희 랩탑 컴퓨터의 기능선택방법 및 인터페이스회로

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6757163B2 (en) 2001-11-19 2004-06-29 Samsung Electronics Co., Ltd. Computer having an auxiliary memory unit removably fastened on a fixing bracket

Also Published As

Publication number Publication date
EP0585554A1 (en) 1994-03-09
JPH0678775A (ja) 1994-03-22
DE69306067T2 (de) 1997-06-12
DE69306067D1 (de) 1997-01-02
KR950012900B1 (ko) 1995-10-23
JP2522151B2 (ja) 1996-08-07
EP0585554B1 (en) 1996-11-20

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