ES8602132A1 - Procedimiento para introducir dna foraneo en algas verdes - Google Patents

Procedimiento para introducir dna foraneo en algas verdes

Info

Publication number
ES8602132A1
ES8602132A1 ES527131A ES527131A ES8602132A1 ES 8602132 A1 ES8602132 A1 ES 8602132A1 ES 527131 A ES527131 A ES 527131A ES 527131 A ES527131 A ES 527131A ES 8602132 A1 ES8602132 A1 ES 8602132A1
Authority
ES
Spain
Prior art keywords
dna
agrobacterium
foreign genes
green algae
introducing foreign
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES527131A
Other languages
English (en)
Other versions
ES527131A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Standard Oil Co
Original Assignee
Standard Oil Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Standard Oil Co filed Critical Standard Oil Co
Publication of ES8602132A1 publication Critical patent/ES8602132A1/es
Publication of ES527131A0 publication Critical patent/ES527131A0/es
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

PROCEDIMIENTO PARA INTRODUCIR DNA FORANEO EN ALGAS VERDES. COMPRENDE: A) INFECTAR UNA CELULA DE ALGAS VERDES, ELEGIDA EN LAS ESPECIES CHLAMYDOMONAS REINHARDTII, PROTOSIPHON BOTRYOIDES Y CHLORELLA PYRENOIDOSA, CON AGROBACTERIUM TUMEFACIENS O AGROBACTERIUM RHIZOGENES GENERICAMENTE ALTERADO QUE CONTIENE UN GEN FORANEO BIOLOGICAMENTE FUNCIONAL INCORPORADO DENTRO DE LA SECUENCIA T-DNA, PROTOCARIOTICO, ENCARIOTICO, O UNA MEZCLA DE AMBOS, DEL PLASMIDO BACTERIAL, Y QUE COMPRENDE UN MARCADOR DE SELECCION; B) DIVIDIR ACTIVAMENTE EL AGROBACTERIUM Y LA CELULA DE ALGAS VERDES; Y C) INCUBAR A TEMPERATURA INFERIOR A 32C PARA LOGRAR EL CRECIMIENTO DE CELULAS DE ALGAS SOBRE EL MEDIO DE CRECIMIENTO ALGAL, DURANTE UN PERIODO DE TIEMPO SUFICIENTE PARA PERMITIR LA INFECCION DE LA CELULA ALGAL COMPRENDIDO ENTRE 30 SEGUNDOS Y 15 MINUTOS.
ES527131A 1982-11-08 1983-11-08 Procedimiento para introducir dna foraneo en algas verdes Granted ES527131A0 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US43973682A 1982-11-08 1982-11-08

Publications (2)

Publication Number Publication Date
ES8602132A1 true ES8602132A1 (es) 1985-11-16
ES527131A0 ES527131A0 (es) 1985-11-16

Family

ID=23745926

Family Applications (1)

Application Number Title Priority Date Filing Date
ES527131A Granted ES527131A0 (es) 1982-11-08 1983-11-08 Procedimiento para introducir dna foraneo en algas verdes

Country Status (5)

Country Link
EP (1) EP0108580A1 (es)
JP (1) JPS5998692A (es)
DK (1) DK509283A (es)
ES (1) ES527131A0 (es)
IL (1) IL70022A0 (es)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4956282A (en) * 1985-07-29 1990-09-11 Calgene, Inc. Mammalian peptide expression in plant cells
US6774283B1 (en) 1985-07-29 2004-08-10 Calgene Llc Molecular farming
AU616635B2 (en) 1986-04-02 1991-11-07 Pioneer Hi-Bred International, Inc. Virus resistant plants having coat protein
WO1990000193A1 (en) * 1988-06-29 1990-01-11 Plantoma Corporation Sustained autotrophic production of plant secondary metabolites
CA2024811A1 (en) 1989-02-24 1990-08-25 David A. Fischhoff Synthetic plant genes and method for preparation
US5270175A (en) * 1991-07-12 1993-12-14 Dna Plant Technology Corporation Methods and compositions for producing metabolic products for algae
US6673537B1 (en) 1992-08-19 2004-01-06 Board Of Regents, University Of Nebraska-Lincoln Promoters from chlorella virus genes providing for expression of genes in prokaryotic and eukaryotic hosts
US5563328A (en) 1992-08-19 1996-10-08 Board Of Regents, University Of Nebraska-Lincoln Promoters from chlorella virus genes providing for expression of genes in prokaryotic and eukaryotic hosts
US5661017A (en) * 1993-09-14 1997-08-26 Dunahay; Terri Goodman Method to transform algae, materials therefor, and products produced thereby
US6027900A (en) * 1996-04-12 2000-02-22 Carnegie Institution Of Washington Methods and tools for transformation of eukaryotic algae
US7161064B2 (en) 1997-08-12 2007-01-09 North Carolina State University Method for producing stably transformed duckweed using microprojectile bombardment
PT1276885E (pt) 2000-04-21 2007-10-26 Martek Biosciences Corp Conversão trófica de algas fototróficas estritas, através de engenharia metabólica
US20030022359A1 (en) 2000-06-20 2003-01-30 Sayre Richard T. Transgenic algae for delivering antigens to an animal
AU2002255851B2 (en) * 2001-03-23 2008-03-06 Advanced Bionutrition Microbial feeds for aquaculture and agriculture
KR101034822B1 (ko) * 2008-04-23 2011-05-16 성균관대학교산학협력단 해마토코쿠스 플루비알리스의 형질 전환 방법, 이를 이용하여 형성된 형질 전환 균주 및 단백질
US8435790B2 (en) 2008-07-25 2013-05-07 The Regents Of The University Of California Methods of modulating lipid concentrations in eukaryotic cells
US20100183523A1 (en) 2009-01-22 2010-07-22 Wagner Richard E Dental composition and method

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1211017B (de) * 1964-09-11 1966-02-17 Yakult Honsha Fa Kk Verfahren zum Zuechten von einzelligen Gruenalgen, wie Chlorella
US4259444A (en) * 1972-06-07 1981-03-31 General Electric Company Microorganisms having multiple compatible degradative energy-generating plasmids and preparation thereof
US4237224A (en) * 1974-11-04 1980-12-02 Board Of Trustees Of The Leland Stanford Jr. University Process for producing biologically functional molecular chimeras
IT1109506B (it) * 1978-05-23 1985-12-16 Snam Progetti Processo enzimatico-microbiologico per la produzione di amminoacidi otticamente attivi a partire da idantoine e/o carbamil-derivati racemi

Also Published As

Publication number Publication date
JPS5998692A (ja) 1984-06-07
DK509283D0 (da) 1983-11-07
IL70022A0 (en) 1984-01-31
EP0108580A1 (en) 1984-05-16
DK509283A (da) 1984-05-09
ES527131A0 (es) 1985-11-16

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