EA199800324A1 - Улучшенное встраивание экзогенной днк, введенной в эукариостические клетки - Google Patents

Улучшенное встраивание экзогенной днк, введенной в эукариостические клетки

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Publication number
EA199800324A1
EA199800324A1 EA199800324A EA199800324A EA199800324A1 EA 199800324 A1 EA199800324 A1 EA 199800324A1 EA 199800324 A EA199800324 A EA 199800324A EA 199800324 A EA199800324 A EA 199800324A EA 199800324 A1 EA199800324 A1 EA 199800324A1
Authority
EA
Eurasian Patent Office
Prior art keywords
exogenous dna
eukaryotic cell
cells
proteins
eucariostic
Prior art date
Application number
EA199800324A
Other languages
English (en)
Other versions
EA001039B1 (ru
Inventor
Дженевив Хансен
Мэри-Делл Чилтон
Original Assignee
Новартис Аг
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Новартис Аг filed Critical Новартис Аг
Publication of EA199800324A1 publication Critical patent/EA199800324A1/ru
Publication of EA001039B1 publication Critical patent/EA001039B1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/14—Plant cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

В заявке описан улучшенный способ достижения стабильной интеграции фрагмента экзогенной ДНК в интактной форме в геном эукариотической клетки, в частности растительной клетки. Способ предусматривает введение экзогенной ДНК вместе с одним или несколькими протеинами, которые обеспечивают интеграцию экзогенной ДНК в эукариотическую клетку, предназначенную для трансформации, причем протеины вводят в форме химерного гена или транслируемой РНК, предназначенной для экспрессии в эукариотической клетке. Способ, в частности, применим к растительным клеткам для достижения стабильной интеграции фрагмента экзогенной ДНК, связанного с пограничными последовательностями Т-ДНК, в интактной форме с использованием обеспечивающих интеграцию протеинов, происходящих из Agrobacterium. Из клеток, трансформированных в соответствии со способом по изобретению, могут быть получены трансгенные культуры, ткани и целые организмы, в частности трансгенные растения.Международная заявка была опубликована вместе с отчетом о международном поиске.
EA199800324A 1995-09-25 1996-09-12 Способ интеграции экзогенной днк в геном растительной клетки и способ получения фертильного трансгенного растения с интегрированной в геном экзогенной днк EA001039B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US422095P 1995-09-25 1995-09-25
PCT/EP1996/004006 WO1997012046A1 (en) 1995-09-25 1996-09-12 Improved integration of exogenous dna delivered to eukaryotic cells

Publications (2)

Publication Number Publication Date
EA199800324A1 true EA199800324A1 (ru) 1998-10-29
EA001039B1 EA001039B1 (ru) 2000-08-28

Family

ID=21709739

Family Applications (1)

Application Number Title Priority Date Filing Date
EA199800324A EA001039B1 (ru) 1995-09-25 1996-09-12 Способ интеграции экзогенной днк в геном растительной клетки и способ получения фертильного трансгенного растения с интегрированной в геном экзогенной днк

Country Status (14)

Country Link
EP (1) EP0853675B1 (ru)
JP (1) JPH11511336A (ru)
KR (1) KR100477413B1 (ru)
CN (1) CN1193097C (ru)
AT (1) ATE321142T1 (ru)
AU (1) AU707948B2 (ru)
BR (1) BR9610738A (ru)
CA (1) CA2230966C (ru)
DE (1) DE69635955T2 (ru)
EA (1) EA001039B1 (ru)
ES (1) ES2262158T3 (ru)
HU (1) HU227410B1 (ru)
PL (1) PL191113B1 (ru)
WO (1) WO1997012046A1 (ru)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6153811A (en) * 1997-12-22 2000-11-28 Dekalb Genetics Corporation Method for reduction of transgene copy number
GB9816138D0 (en) 1998-07-24 1998-09-23 Ciba Geigy Ag Organic compounds
US7122716B2 (en) 1999-09-15 2006-10-17 Purdue Research Foundation Enhanced plant cell transformation by addition of host genes involved in T-DNA integration
AU782896B2 (en) * 1999-09-15 2005-09-08 Purdue Research Foundation Enhanced plant cell transformation by addition of host genes involved in T-DNA integration
US6696622B1 (en) 1999-09-15 2004-02-24 Purdue Research Foundation Enhanced plant cell transformation by addition of host genes involved in T-DNA integration
EP1238090B1 (en) 1999-12-16 2007-05-09 CropDesign N.V. Optimized t-dna transfer and vectors therefor
GB2359812A (en) * 2000-03-01 2001-09-05 Novartis Res Foundation Delivery method
US7468475B2 (en) 2000-06-16 2008-12-23 Schmuelling Thomas Method for modifying plant morphology, biochemistry and physiology
US7279336B2 (en) 2000-09-14 2007-10-09 Gelvin Stanton B Methods and compositions for enhanced plant cell transformation
US7534934B2 (en) 2002-02-20 2009-05-19 J.R. Simplot Company Precise breeding
US20050229267A1 (en) 2002-02-20 2005-10-13 J.R. Simplot Company Precise breeding
CA2615797C (en) 2005-07-18 2014-04-29 Pioneer Hi-Bred International, Inc. Modified frt recombination sites and methods of use
AU2007302306A1 (en) 2006-09-25 2008-04-03 Freie Universitat Berlin Transcriptional repressors of cytokinin signaling and their use
CN105002209A (zh) * 2015-08-15 2015-10-28 石河子大学 一种提高棉花转基因效率的方法
CN105969793A (zh) * 2016-05-10 2016-09-28 广西兆和种业有限公司 一种水稻育种方法
AU2019275110A1 (en) 2018-05-24 2020-10-22 Monsanto Technology Llc Genome editing in plants

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5932782A (en) * 1990-11-14 1999-08-03 Pioneer Hi-Bred International, Inc. Plant transformation method using agrobacterium species adhered to microprojectiles
DE4309203C1 (de) * 1993-03-22 1994-04-21 Holt Claus Von Prof Dr Verfahren zur Produktion von transgenischen Pflanzen
WO1995005471A2 (en) * 1993-08-13 1995-02-23 Ciba-Geigy Ag Method for stable transformation of plants
DE4344929A1 (de) * 1993-12-31 1995-07-06 Fred Dr Jasper In vitro Transformationskomplex

Also Published As

Publication number Publication date
DE69635955D1 (de) 2006-05-11
AU7128596A (en) 1997-04-17
PL191113B1 (pl) 2006-03-31
WO1997012046A1 (en) 1997-04-03
EP0853675B1 (en) 2006-03-22
CN1197482A (zh) 1998-10-28
JPH11511336A (ja) 1999-10-05
CN1193097C (zh) 2005-03-16
ATE321142T1 (de) 2006-04-15
EA001039B1 (ru) 2000-08-28
AU707948B2 (en) 1999-07-22
DE69635955T2 (de) 2006-08-31
MX9802312A (es) 1998-08-30
EP0853675A1 (en) 1998-07-22
CA2230966A1 (en) 1997-04-03
KR100477413B1 (ko) 2006-01-27
KR19990063713A (ko) 1999-07-26
ES2262158T3 (es) 2006-11-16
PL326087A1 (en) 1998-08-17
CA2230966C (en) 2010-08-24
BR9610738A (pt) 1999-07-13
HU227410B1 (hu) 2011-05-30
HUP9900451A3 (en) 2001-11-28
HUP9900451A2 (hu) 1999-05-28

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MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): AM AZ BY KZ KG MD TJ TM

PC4A Registration of transfer of a eurasian patent by assignment
MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): RU