KR930703449A - 융합 ms-2 코트 단백질을 포함하는 항원-제공 캡시드 - Google Patents
융합 ms-2 코트 단백질을 포함하는 항원-제공 캡시드Info
- Publication number
- KR930703449A KR930703449A KR1019930702199A KR930702199A KR930703449A KR 930703449 A KR930703449 A KR 930703449A KR 1019930702199 A KR1019930702199 A KR 1019930702199A KR 930702199 A KR930702199 A KR 930702199A KR 930703449 A KR930703449 A KR 930703449A
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- Prior art keywords
- protein
- capsid
- coat protein
- amino acid
- chimeric protein
- Prior art date
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- 101710132601 Capsid protein Proteins 0.000 title claims abstract 8
- 101710094648 Coat protein Proteins 0.000 title claims abstract 8
- 102100021181 Golgi phosphoprotein 3 Human genes 0.000 title claims abstract 8
- 101710125418 Major capsid protein Proteins 0.000 title claims abstract 8
- 101710141454 Nucleoprotein Proteins 0.000 title claims abstract 8
- 101710083689 Probable capsid protein Proteins 0.000 title claims abstract 8
- 210000000234 capsid Anatomy 0.000 title claims abstract 7
- 230000004927 fusion Effects 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 8
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 6
- 102000037865 fusion proteins Human genes 0.000 claims abstract 8
- 108020001507 fusion proteins Proteins 0.000 claims abstract 8
- 239000012634 fragment Substances 0.000 claims abstract 3
- 229960005486 vaccine Drugs 0.000 claims abstract 2
- 239000013604 expression vector Substances 0.000 claims 4
- 150000001413 amino acids Chemical group 0.000 claims 3
- 239000002299 complementary DNA Substances 0.000 claims 3
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims 2
- 238000000034 method Methods 0.000 claims 2
- 239000000203 mixture Substances 0.000 claims 2
- 241000588724 Escherichia coli Species 0.000 claims 1
- 239000004471 Glycine Substances 0.000 claims 1
- 108091034117 Oligonucleotide Proteins 0.000 claims 1
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 claims 1
- 239000004473 Threonine Substances 0.000 claims 1
- 125000000539 amino acid group Chemical group 0.000 claims 1
- 238000009472 formulation Methods 0.000 claims 1
- 125000003630 glycyl group Chemical group [H]N([H])C([H])([H])C(*)=O 0.000 claims 1
- 230000001939 inductive effect Effects 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 108091008146 restriction endonucleases Proteins 0.000 claims 1
- 238000012163 sequencing technique Methods 0.000 claims 1
- 239000013598 vector Substances 0.000 claims 1
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 2
- 239000013078 crystal Substances 0.000 abstract 1
- 230000010354 integration Effects 0.000 abstract 1
- 238000000635 electron micrograph Methods 0.000 description 2
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
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- C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/14—Antivirals for RNA viruses
- A61P31/16—Antivirals for RNA viruses for influenza or rhinoviruses
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/14—Antivirals for RNA viruses
- A61P31/18—Antivirals for RNA viruses for HIV
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- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
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- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
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- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
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- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K39/00—Medicinal preparations containing antigens or antibodies
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
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- C07K2319/00—Fusion polypeptide
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
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- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/735—Fusion polypeptide containing domain for protein-protein interaction containing a domain for self-assembly, e.g. a viral coat protein (includes phage display)
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- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16111—Human Immunodeficiency Virus, HIV concerning HIV env
- C12N2740/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
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Abstract
본 발명은 키메라 단백질에 관한 것으로, 이 단백질은 백신에의 통합에 적당하며 캡시드 어셈블리의 일부분을 형성할 수 있고, 파지 MS-2의 코트 단백질의 아미노산 서열, 또는 그것의 보존적으로 변형된 변이체, 또는 캡시드-형성 능력을 보유하기에 충분한 상기 서열의 단편 또는 변이체를 포함하며, 상기 아미노산 서열은 무상 파지의 결정구조에서 볼 수 있는 바와같이 캡시드 어셈블리의 용기 단백질 헤어핀 구조에 상응하는 영역에 의해 에피토프를 삽입함으로써 변형되었다.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 SDS-PAGE및 웨스턴 블롯에 의한 카메라 MS-2 단백질을 확인하는 도면, 제2도는 야생형 MS-2에 대한 전자 현미경 사진, 제3도는 MS2-캡시드에 대한 전자 현미경 사진.
Claims (11)
- 캡시드 어셈블리의 일부분을 형성할 수 있고, 파지 MS-2의 코트 단백질의 아미노산 서열 또는 그것의 보존적으로 변형된 변이체, 또는 캡시드-형성 능력을 보유하기에 충분한 상기 서열의 단편 또는 변이체를 포함하는 키메라 단백질로서, 상기 아미노산 서열은 코트 단백질의 N-말단 융기 b-헤어핀 구조에 해당하는 영역에 외래 에피토프를 삽입시킴으로써 변형된 것을 특징으로 하는 키메라 단백질.
- 제1항에 있어서, 파지 MS-2의 코트 단백질의 아미노산 서열을 포함하는 키메라 단백질.
- 제1항 또는 2항에 있어서, 외래 에피토프는 MS-2의 코트 단백질 서열에 관련하여 아미노산 잔기 1내지 20의 영역에 삽입된 것을 특징으로 하는 키메라 단백질.
- 제3항에 있어서, 외래 에피토프가 글리신 14와 트레오닌 15의 영역에 삽입된 것을 특징으로 하는 키메라 단백질.
- 전술한 항중 어느 한 항에 있어서, 외래 에피토프 바로 위쪽 및 아래쪽 영역에 하나 또는 그 이상의 글리신 잔기를 포함하는 키메라 단백질.
- 전술한 항중 어느 한 항의 키메라 단백질 또는 이러한 단백질들의 혼합물을 포함하는 캡시드.
- 제1항 내지 5항중 어느 한 항의 키메라 단백질의 또는 제6항의 캡시드의 제조방법으로서, MS-2의 코트단백질에 상응하는 cDNA서열, 또는 이러한 코트 단백질의 보존적으로 변형된 변이체 또는 캡시드-형성 능력을 보유하기에 충분한 상기 단백질의 단편 또는 변이체에 상응하는 cDNA서열을, 부위 특정 돌연별이 생성에 적당한 서열화 벡터에 도입시키는 단계, 융기 헤어핀 구조를 코드하는 cDNA의 영역에 독특한 제한효소 인지 부위를 생성시키는 단계, 외래 에피토프를 포함하고 있는 올리고누클레오티드를 제한부위에 도입시키고, 하위 클로닝함으로써 변형된 유전자를 적당한 발현벡터안에 도입시키는 단계, 그리고 적당한 숙주에서 외래 유전자를 내포하는 변형된 발현 벡터로 부터의 발현을 유도하는 단계로 이루어지는 방법.
- 제7항에 있어서, 숙주가 대장균임을 특징으로 하는 방법.
- 제1항 내지 6항중 어느 한 항의 키메라 단백질을 코드하는 DNA를 포함하는 발현벡터.
- 제9항의 발현벡터에 의해 형질전환된 숙주.
- 제1항 내지 5항중 어느 한 항의 단백질 또는 제 6항의 캡시드를 포함하는 백신제제.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB910550.3 | 1991-01-24 | ||
GB919101550A GB9101550D0 (en) | 1991-01-24 | 1991-01-24 | Antigen-presenting chimaeric protein |
PCT/GB1992/000124 WO1992013081A1 (en) | 1991-01-24 | 1992-01-22 | Antigen-presenting capsid with fusion ms2-coat protein |
Publications (2)
Publication Number | Publication Date |
---|---|
KR930703449A true KR930703449A (ko) | 1993-11-30 |
KR100197819B1 KR100197819B1 (ko) | 1999-06-15 |
Family
ID=10688936
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019930702199A KR100197819B1 (ko) | 1991-01-24 | 1992-01-22 | 융합 ms2 코트 단백질을 포함하는 항원제공 캡시드 |
Country Status (15)
Country | Link |
---|---|
US (1) | US5534257A (ko) |
EP (1) | EP0572433B1 (ko) |
JP (1) | JP3187832B2 (ko) |
KR (1) | KR100197819B1 (ko) |
AT (1) | ATE201449T1 (ko) |
AU (1) | AU653387B2 (ko) |
CA (1) | CA2101070C (ko) |
DE (1) | DE69231837T8 (ko) |
FI (1) | FI110615B (ko) |
GB (2) | GB9101550D0 (ko) |
IE (1) | IE920200A1 (ko) |
NO (1) | NO313917B1 (ko) |
NZ (1) | NZ241373A (ko) |
WO (1) | WO1992013081A1 (ko) |
ZA (1) | ZA92511B (ko) |
Families Citing this family (41)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB9114003D0 (en) * | 1991-06-28 | 1991-08-14 | Mastico Robert A | Chimaeric protein |
US5837268A (en) | 1991-10-16 | 1998-11-17 | University Of Saskatchewan | GnRH-leukotoxin chimeras |
WO1995005454A1 (en) * | 1992-02-22 | 1995-02-23 | Cambridge Bacteriophage Technologies Ltd. | Engineered bacteriophages and vaccines containing them |
GB9213601D0 (en) * | 1992-06-26 | 1992-08-12 | Mastico Robert A | Protein based delivery system |
US5559099A (en) * | 1994-09-08 | 1996-09-24 | Genvec, Inc. | Penton base protein and methods of using same |
US6465253B1 (en) | 1994-09-08 | 2002-10-15 | Genvec, Inc. | Vectors and methods for gene transfer to cells |
US5846782A (en) * | 1995-11-28 | 1998-12-08 | Genvec, Inc. | Targeting adenovirus with use of constrained peptide motifs |
US5770442A (en) * | 1995-02-21 | 1998-06-23 | Cornell Research Foundation, Inc. | Chimeric adenoviral fiber protein and methods of using same |
US6127525A (en) * | 1995-02-21 | 2000-10-03 | Cornell Research Foundation, Inc. | Chimeric adenoviral coat protein and methods of using same |
US6783980B2 (en) | 1995-06-15 | 2004-08-31 | Crucell Holland B.V. | Packaging systems for human recombinant adenovirus to be used in gene therapy |
JP3421970B2 (ja) | 1996-03-01 | 2003-06-30 | ノバルティス アクチエンゲゼルシャフト | ペプチド免疫原 |
US5766905A (en) * | 1996-06-14 | 1998-06-16 | Associated Universities Inc. | Cytoplasmic bacteriophage display system |
US20030017138A1 (en) | 1998-07-08 | 2003-01-23 | Menzo Havenga | Chimeric adenoviruses |
IL140918A0 (en) | 1998-07-27 | 2002-02-10 | Genentech Inc | Improved transformation efficiency in phage display through modification of a coat protein |
US6929946B1 (en) | 1998-11-20 | 2005-08-16 | Crucell Holland B.V. | Gene delivery vectors provided with a tissue tropism for smooth muscle cells, and/or endothelial cells |
US6492169B1 (en) | 1999-05-18 | 2002-12-10 | Crucell Holland, B.V. | Complementing cell lines |
US6913922B1 (en) | 1999-05-18 | 2005-07-05 | Crucell Holland B.V. | Serotype of adenovirus and uses thereof |
JP2003516755A (ja) * | 1999-12-15 | 2003-05-20 | ジェネンテック・インコーポレーテッド | ショットガン走査、すなわち機能性タンパク質エピトープをマッピングするための組み合わせ方法 |
US7235233B2 (en) | 2000-09-26 | 2007-06-26 | Crucell Holland B.V. | Serotype 5 adenoviral vectors with chimeric fibers for gene delivery in skeletal muscle cells or myoblasts |
US7128911B2 (en) | 2001-01-19 | 2006-10-31 | Cytos Biotechnology Ag | Antigen arrays for treatment of bone disease |
US7094409B2 (en) | 2001-01-19 | 2006-08-22 | Cytos Biotechnology Ag | Antigen arrays for treatment of allergic eosinophilic diseases |
EP1450856B1 (en) | 2001-09-14 | 2009-11-11 | Cytos Biotechnology AG | Packaging of immunostimulatory cpg into virus-like particles: method of preparation and use |
MXPA04002644A (es) | 2001-10-05 | 2004-07-08 | Cytos Biotechnology Ag | Conjugados que portan un peptido de angiotensina y usos de los mismos. |
US7115266B2 (en) | 2001-10-05 | 2006-10-03 | Cytos Biotechnology Ag | Angiotensin peptide-carrier conjugates and uses thereof |
JP4495587B2 (ja) | 2002-04-25 | 2010-07-07 | クルセル ホランド ベー ヴェー | 組換えアデノウイルスベクターおよびその使用 |
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WO2004084940A1 (en) | 2003-03-26 | 2004-10-07 | Cytos Biotechnology Ag | Packaging of immunostimulatory oligonucleotides into virus-like particles: method of preparation and use |
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EP1812051A2 (en) * | 2004-10-05 | 2007-08-01 | Cytos Biotechnology AG | Vlp-antigen conjugates and their uses as vaccines |
EP1746165A1 (en) * | 2005-07-21 | 2007-01-24 | Cytos Biotechnology AG | Scalable fermentation process |
CN101213294B (zh) * | 2005-05-26 | 2013-03-27 | 赛托斯生物技术公司 | 可放大的发酵方法 |
EP1736538A1 (en) | 2005-06-21 | 2006-12-27 | Cytos Biotechnology AG | Process for the preparative purification of virus-like-particles (VLPs) |
EP2431468A1 (en) | 2005-09-28 | 2012-03-21 | Cytos Biotechnology AG | Interleukin-1 conjugates and uses thereof |
EP1973608A1 (en) | 2005-12-14 | 2008-10-01 | Cytos Biotechnology AG | Immunostimulatory nucleic acid packaged particles for the treatment of hypersensitivity |
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SG172696A1 (en) | 2006-06-12 | 2011-07-28 | Cytos Biotechnology Ag | Processes for packaging oligonucleotides into virus-like particles of rna bacteriophages |
EP2554664A1 (de) | 2011-08-02 | 2013-02-06 | Life Science Inkubator | Verfahren zur Aufreinigung von Virus-ähnlichen Partikeln (VLP) |
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AU637049B2 (en) * | 1988-05-05 | 1993-05-20 | American Cyanamid Company | Recombinant flagellin vaccines |
IT1226551B (it) * | 1988-07-29 | 1991-01-24 | Sclavo Spa | Peptide sintetico immunologicamente attivo capace di indurre la produzione di anticorpi con elevata specificita' verso l'alfa- fetoproteina e loro impiego in campo diagnostico |
AU629237B2 (en) * | 1988-08-30 | 1992-10-01 | Applied Research Systems Ars Holding N.V. | Recombinant fusion proteins for altering hormone secretion |
WO1990014355A1 (en) * | 1989-05-17 | 1990-11-29 | Research Corporation Technologies, Inc. | Retrovirus-mediated secretion of recombinant products |
-
1991
- 1991-01-24 GB GB919101550A patent/GB9101550D0/en active Pending
-
1992
- 1992-01-22 JP JP50357692A patent/JP3187832B2/ja not_active Expired - Fee Related
- 1992-01-22 AU AU11737/92A patent/AU653387B2/en not_active Ceased
- 1992-01-22 GB GB9201372A patent/GB2253626B/en not_active Expired - Fee Related
- 1992-01-22 US US08/090,148 patent/US5534257A/en not_active Expired - Fee Related
- 1992-01-22 KR KR1019930702199A patent/KR100197819B1/ko not_active IP Right Cessation
- 1992-01-22 EP EP92903356A patent/EP0572433B1/en not_active Expired - Lifetime
- 1992-01-22 CA CA002101070A patent/CA2101070C/en not_active Expired - Fee Related
- 1992-01-22 DE DE69231837T patent/DE69231837T8/de not_active Expired - Fee Related
- 1992-01-22 AT AT92903356T patent/ATE201449T1/de not_active IP Right Cessation
- 1992-01-22 WO PCT/GB1992/000124 patent/WO1992013081A1/en active IP Right Grant
- 1992-01-23 IE IE020092A patent/IE920200A1/en not_active Application Discontinuation
- 1992-01-23 NZ NZ241373A patent/NZ241373A/xx unknown
- 1992-01-24 ZA ZA92511A patent/ZA92511B/xx unknown
-
1993
- 1993-07-22 NO NO19932644A patent/NO313917B1/no not_active IP Right Cessation
- 1993-07-23 FI FI933328A patent/FI110615B/fi active
Also Published As
Publication number | Publication date |
---|---|
JPH06504907A (ja) | 1994-06-09 |
ATE201449T1 (de) | 2001-06-15 |
CA2101070C (en) | 2003-04-01 |
EP0572433A1 (en) | 1993-12-08 |
AU1173792A (en) | 1992-08-27 |
US5534257A (en) | 1996-07-09 |
GB2253626B (en) | 1995-02-08 |
GB9201372D0 (en) | 1992-03-11 |
GB2253626A (en) | 1992-09-16 |
DE69231837T2 (de) | 2001-10-18 |
NZ241373A (en) | 1993-04-28 |
FI110615B (fi) | 2003-02-28 |
ZA92511B (en) | 1992-11-25 |
DE69231837T8 (de) | 2004-08-12 |
NO313917B1 (no) | 2002-12-23 |
IE920200A1 (en) | 1992-07-29 |
GB9101550D0 (en) | 1991-03-06 |
NO932644L (no) | 1993-09-23 |
CA2101070A1 (en) | 1992-07-25 |
DE69231837D1 (de) | 2001-06-28 |
JP3187832B2 (ja) | 2001-07-16 |
FI933328A0 (fi) | 1993-07-23 |
EP0572433B1 (en) | 2001-05-23 |
KR100197819B1 (ko) | 1999-06-15 |
NO932644D0 (no) | 1993-07-22 |
WO1992013081A1 (en) | 1992-08-06 |
AU653387B2 (en) | 1994-09-29 |
FI933328A (fi) | 1993-09-07 |
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