KR920700295A - 고감도 발광분석방법 - Google Patents

고감도 발광분석방법

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Publication number
KR920700295A
KR920700295A KR1019900702683A KR900702683A KR920700295A KR 920700295 A KR920700295 A KR 920700295A KR 1019900702683 A KR1019900702683 A KR 1019900702683A KR 900702683 A KR900702683 A KR 900702683A KR 920700295 A KR920700295 A KR 920700295A
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South Korea
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same
cnh
previous definition
phenyl
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KR1019900702683A
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KR960002875B1 (ko
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요시히로 오야마
슌타로오 호사카
데쯔야 마키노
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마에다 가쯔노스께
도오레 가부시기가이샤
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Publication of KR920700295A publication Critical patent/KR920700295A/ko
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Publication of KR960002875B1 publication Critical patent/KR960002875B1/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Nitrogen And Oxygen As The Only Ring Hetero Atoms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
  • Luminescent Compositions (AREA)
  • Plural Heterocyclic Compounds (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

내용 없음

Description

고감도 발광분석방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도 및 제2도는 본 발명의 엔헨서인 2－히드록시－9－플루올레논과 공지의 앤헨서를 이용하여, CA 15－3의 분석을 행한 결과를 비교한 것이고,
제1도는 5초후의 발광강도의 SN비를,
제2도는 10초후의 발광강도의 SN비를 각각 나타내고,
제3도는 본 발명의 엔헨서를 발광계에 첨가하지 않은 경우로써, CA 15－3의 분석을 행하여, 결과를 비교한 것이다.

Claims (4)

  1. (a)페르옥시다아제 또는 페르옥시다아제 유도체, (b)산화제 및 (c)루미놀이소루미놀 또는 그들의 유도체의 반응에 의해 생기는 화학발광을 이용하여, 물질을 검출 또는 측정하는 방법에 있어서, 그 발광반응을 2－히드록시－9－플루올레논,
    〔식중, R1은 수소, CnH2n+1(여기서, n은 1-4의 정수를 나타낸다), XCnH2n(여기서, X는 F, Cl, Br 또는 I를 나타내고, n=1-4의 양의 정수를 나타낸다), CnH2n+1CO2(n은 전기정의와 동일), 페닐기, 나프틸기, CnH2n+1C6H4(n은 전기정의와 동일), YC6H4(Y는 F, Cl, Br, I 또는 페닐기를 나타낸다), XYC6H3(X, Y는 전기정의와 동일)〕을 나타낸다로 표시되는 옥사졸 유도체로서 이루어지는 군에서 선택된 적어도 하나의 화합물의 존재하에 행해지는 것을 특징으로 하는 고감도 발광분석방법.
  2. 제3항에 있어서, 일반식〔Ⅰ〕에 나타나는 화합물의 R1이 수소, 메틸기, 클로로멜틸기, 에톡시카르보닐기, 페닐기, 브로모페닐기, 메틸페닐기, 클로로페닐기, 나프틸기 또는 디클로로페닐기인 것을 특징으로 하는 고감동 발광분석방법.
  3. 일반식
    〔식중, R2는 XCnH2n(여기서, X는 F, Cl, Br 또는 I를 나타내고, n=1-4의 양의 정수를 나타낸다), CnH2n+1CO2(n은 전기정의와 동일), 페닐기, 나프틸기, CnH2n+1C6H4(n은 전기정의와 동일), YC6H4(Y는 F, Cl, Br, I 또는 페닐기를 나타낸다), XYC6H3(X, Y는 전기정의와 동일)을 나타낸다〕로 표시되는 옥사졸 유도체.
  4. 제3항에 있어서, 일반식〔Ⅱ〕로 표시되는 화합물의 R2가 클로로멜틸기, 에톡시카르보닐기, 브로모페닐기, 클로로페닐기, 나프틸기, 디클로로페닐기인 또는 메틸페닐기인 것을 특징으로 하는 신규한 옥사졸 유도체.
    ※ 참고사항 : 최초출원 내용에 의하여 공개되는 것임.
KR1019900702683A 1989-04-28 1990-04-27 옥사졸유도체 및 이것을 이용한 고감도 발광분석방법 KR960002875B1 (ko)

Applications Claiming Priority (10)

Application Number Priority Date Filing Date Title
JP???1-?109598 1989-04-28
JP???1-?109598? 1989-04-28
JP10959889 1989-04-28
JP22957589 1989-09-05
JP???1-?229575? 1989-09-05
JP???1-?229575 1989-09-05
JP3799790 1990-02-19
JP???2-?37997 1990-02-19
JP???2-?37997? 1990-02-19
PCT/JP1990/000558 WO1990013665A1 (fr) 1989-04-28 1990-04-27 Analyse spectrale d'emissions avec degre de sensibilite eleve

Publications (2)

Publication Number Publication Date
KR920700295A true KR920700295A (ko) 1992-02-19
KR960002875B1 KR960002875B1 (ko) 1996-02-27

Family

ID=27289667

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019900702683A KR960002875B1 (ko) 1989-04-28 1990-04-27 옥사졸유도체 및 이것을 이용한 고감도 발광분석방법

Country Status (10)

Country Link
US (1) US5206149A (ko)
EP (1) EP0422252B1 (ko)
JP (1) JPH0786096B1 (ko)
KR (1) KR960002875B1 (ko)
AT (1) ATE125573T1 (ko)
CA (1) CA2032154C (ko)
DE (1) DE69021161T2 (ko)
DK (1) DK0422252T3 (ko)
ES (1) ES2074571T3 (ko)
WO (1) WO1990013665A1 (ko)

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Also Published As

Publication number Publication date
DK0422252T3 (da) 1995-09-11
ATE125573T1 (de) 1995-08-15
ES2074571T3 (es) 1995-09-16
DE69021161D1 (de) 1995-08-31
JPH0786096B1 (ko) 1995-09-20
CA2032154A1 (en) 1990-10-29
KR960002875B1 (ko) 1996-02-27
WO1990013665A1 (fr) 1990-11-15
EP0422252A1 (en) 1991-04-17
CA2032154C (en) 1996-01-09
EP0422252B1 (en) 1995-07-26
DE69021161T2 (de) 1995-11-30
US5206149A (en) 1993-04-27
EP0422252A4 (en) 1992-04-08

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