KR920012449A - 고정화된 d-아미노산 옥시다제 및 약물 제조를 위한 이의 용도 - Google Patents

고정화된 d-아미노산 옥시다제 및 약물 제조를 위한 이의 용도 Download PDF

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KR920012449A
KR920012449A KR1019910024113A KR910024113A KR920012449A KR 920012449 A KR920012449 A KR 920012449A KR 1019910024113 A KR1019910024113 A KR 1019910024113A KR 910024113 A KR910024113 A KR 910024113A KR 920012449 A KR920012449 A KR 920012449A
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amino acid
acid oxidase
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vinyl
unit
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KR1019910024113A
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자우베르 클라우스
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테르가우, 라피체
훽스트 아크티엔게젤샤프트
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Publication of KR920012449A publication Critical patent/KR920012449A/ko

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    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/18—Multi-enzyme systems
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    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/082—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained by reactions only involving carbon-to-carbon unsaturated bonds
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0022—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with oxygen as acceptor (1.4.3)
    • C12N9/0024—D-Amino acid oxidase (1.4.3.3)
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    • C12P35/00—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin
    • C12P35/06—Cephalosporin C; Derivatives thereof
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi

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Abstract

내용 없음

Description

고정화된 D-아미노산 옥시다제 및 약물 제조를 위한 이의 용도
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (9)

  1. 지지체 물질은, 비닐 아세테이트 단위 및/또는 비닐 알콜 단위와 가교결합제 단위로 실질적으로 이루어진 가교결합된 공중합체이고, 가교결합제 단위는 하기 일반식(Ⅰ) 및/또는 (Ⅱ)의 공중합된 화합물이며, 가교결합제의 양은 중합체에 대해, 1 내지 60중량%이고, 비닐 아실레이트 단위의 아실옥시 그룹이 그대로 존재하거나, 일부 또는 모두가 하이드록실 그룹으로 가수분해되며, 비이드의 평균 입자크기가 20 내지 800㎛이고 평균 공극 직경이 2 내지 10,000nm 인 다공성 비이드-형태의 물질과 D-아미노산옥시다제를 포함하는 효소-피복된 지지체 물질.
    상기식에서, R1및 R2는 동일하거나 상이할수 있고, 비닐, 1-아실옥시 비닐, 알릴 또는 2-아실옥시알릴이고, A는 탄소수 2 내지 8의 2가 탄화수소 라디칼이며, B는 탄소수 1 내지 8의 2가 탄화수소 라디칼이고, X는 아실옥시그룹인데, 상기 라디칼중 아실옥시 구룹은 탄소수 2 내지 18의 라디칼이다.
  2. 제1항에 있어서, D-아미노산 옥시다제가 효모 또는 곰팡이로부터 생성되는 지지체 물질.
  3. 제1항 또는 제2항에 있어서, D-아미노산 옥시다제 및 카탈라제를 함유하는 지지체 물질.
  4. D-아미노산 옥시다제-함유 용액을, 비닐 아세테이트 단위 및/또는 비닐 알콜 단위와 일반식(Ⅰ) 및/또는 (Ⅱ)의 공중합된 화합물인 가교결합제의 단위(여기서, 가교결합제의 양은 중합체에 대해 1 내지 60중량%이다)로 실질적으로 이루어진 가교결합된 공중합체로부터 제조되고, 비닐 아실레이트 단위의 아실옥시 그룹이 그대로 존재하거나, 일부 또는 모두가 하이드록실 그룹으로 가수분해되며, 비이드의 평균 입자 크기는 20 내지 800㎛이고 평균 공극 직경은 2 내지 10,000nm인 다공성 비이드-형태의 지지체 물질과 함께 배양함을 특징으로 하여, 제1항 또는 제2항에서 청구한 피복된 지지체 물질을 제조하는 방법.
    상기식에서, R1, R2, A, B 및 X는 제1항에서 정의한 바와 같다.]
  5. 제4항에 있어서, 곰팡이 또는 효모로 부터 수득된 D-아미노산 옥시다제-함유 용액을 사용하는 방법.
  6. 제4항 또는 제5항에 있어서, 이온 교환 크로마토 그래피에 의해 정제된 D-아미노산 옥시다제-함유 용액을 사용하는 방법.
  7. 제4항 내지 제6항중 어느 한 항에 있어서, D-아미노산 옥시다제 및 카탈라제-함유 용액을 사용하는 방법.
  8. 제4항 내지 제7항중 어느 한 항에 있어서, 배양이 0℃ 내지 40℃의 온도에서 수행되는 방법.
  9. 제1항 내지 제3항중 어느 한 항에서 청구한 피복된 지지체 물질 및 세팔로스포린 유도체를 사용하여 글루타릴-7-아미노세팔로스포란 산 유도체 또는 α-케토아디필-7-아미노 셀팔로스포란 산 유도체를 제조하는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019910024113A 1990-12-24 1991-12-24 고정화된 d-아미노산 옥시다제 및 약물 제조를 위한 이의 용도 KR920012449A (ko)

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DEP4041755.7 1990-12-24
DE4041755 1990-12-24

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US (1) US5599702A (ko)
EP (1) EP0492495A3 (ko)
JP (1) JPH04365484A (ko)
KR (1) KR920012449A (ko)
AU (1) AU660438B2 (ko)
CA (1) CA2058194A1 (ko)
CZ (1) CZ284454B6 (ko)
MX (1) MX9102792A (ko)
NZ (1) NZ241116A (ko)
TW (1) TW198064B (ko)
ZA (1) ZA9110119B (ko)

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Publication number Priority date Publication date Assignee Title
DE4342770A1 (de) * 1993-12-15 1995-07-06 Boehringer Mannheim Gmbh Trägerfixierte Enzyme
US5980883A (en) * 1996-10-02 1999-11-09 Kuraray Co., Ltd. Polymer gel for medical use
KR100481138B1 (ko) * 1997-09-09 2005-04-11 바이오케미 게젤샤프트 엠베하 효소 활성을 갖는 미생물을 함유하는 구형 입자 및 구형 입자의 제조방법
EP1379531A2 (en) 2001-04-19 2004-01-14 Bioferma Murcia, S.A. Enzymatic process for preparing cephalosporanic acid derivatives using alpha-ketoacid derivatives
EP1754534A1 (de) * 2005-08-03 2007-02-21 MERCK PATENT GmbH Hydrophiles vernetztes Polymer
EP1910433B1 (de) 2005-08-03 2014-11-05 Merck Patent GmbH Hydrophiles vernetztes polymer

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GB1385685A (en) * 1971-04-21 1975-02-26 Glaxo Lab Ltd Cephalosporin derivatives
DE2911192A1 (de) * 1979-03-22 1980-10-02 Boehringer Sohn Ingelheim Neuartiges immobilisiertes glucoseoxidase-katalasepraeparat und seine verwendung zur enzymatischen glucoseoxidation
DE3404021A1 (de) * 1983-05-28 1984-11-29 Hoechst Ag, 6230 Frankfurt Makroporoese perlpolymerisate, verfahren zu ihrer herstellung und ihre anwendung
DE3344912A1 (de) * 1983-12-13 1985-06-20 Hoechst Ag, 6230 Frankfurt Vernetzte polymerisate, verfahren zu ihrer herstellung und ihre anwendung
SE8500157D0 (sv) * 1985-01-11 1985-01-11 Mosbach Klaus Isolation and partial characterization of a d-amino acid oxidase active against cephalosporin c from the yeast trigonopsis variabilis
DE3629177A1 (de) * 1986-08-28 1988-03-17 Hoechst Ag Vernetzte polymerisate und verfahren zu ihrer herstellung
DE3818851A1 (de) * 1988-06-03 1989-12-14 Hoechst Ag Neue transaminase, ihre herstellung und ihre verwendung
DE4028119C1 (ko) * 1990-09-05 1991-12-05 Hoechst Ag, 6230 Frankfurt, De
IT1252308B (it) * 1990-12-21 1995-06-08 Antibioticos Spa Procedimento enzimatico per la produzione di acido 7- amminocefalosporanico e derivati

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CA2058194A1 (en) 1992-06-25
MX9102792A (es) 1992-06-01
AU8989591A (en) 1992-06-25
AU660438B2 (en) 1995-06-29
CS399591A3 (en) 1992-11-18
US5599702A (en) 1997-02-04
JPH04365484A (ja) 1992-12-17
EP0492495A2 (de) 1992-07-01
CZ284454B6 (cs) 1998-12-16
EP0492495A3 (en) 1993-06-16
NZ241116A (en) 1993-11-25
TW198064B (ko) 1993-01-11
ZA9110119B (en) 1992-09-30

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