KR910016928A - 재조합 사람 β-인터페론의 정제방법 - Google Patents

재조합 사람 β-인터페론의 정제방법 Download PDF

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KR910016928A
KR910016928A KR1019910004187A KR910004187A KR910016928A KR 910016928 A KR910016928 A KR 910016928A KR 1019910004187 A KR1019910004187 A KR 1019910004187A KR 910004187 A KR910004187 A KR 910004187A KR 910016928 A KR910016928 A KR 910016928A
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interferon
matrix
concentration
recombinant human
cpg
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KR1019910004187A
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프로타시 오렐로
라뿌올리 파올로
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레나토 스파노
스클라보 에스.피.에이.
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Publication of KR910016928A publication Critical patent/KR910016928A/ko

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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/565—IFN-beta
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents

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Abstract

내용 없음

Description

재조합 사람 β-인터페론의 정제방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 재조합 사람-IFN의 전기영동도이다.

Claims (12)

  1. a) 비정제 상태의 재조합 사람-인터페론 수용액을 탈이온수로 평형화시킨 규토질이 고형 매트릭스와 접촉시켜 재조합 사람-인터페론을 흡착시킨후, 2 내지 10밀리몰/ℓ몰 농도의 아세트산 수용액으로 이를 용출시켜 보다 정제된 수준의 재조합 사람-인터페론을 함유하는 용출 분획을 수거하고, b) 단계 a)에 따라 정재된 제조합 사람-인터페론을 함유하는 용출된 분획을 pH3.0이상의 아세테이트 완충액으로 평형화시킨 양이온 교환수지상에서, 0.1㎝/분 이하의 유속으로 적재시킨후, 매트릭스상에 흡착된 rhu--인터페론을 0.15 내지 0.18몰/ℓ의 NaCl을 함유하는 pH 6.2와 6.8사이의 0.07 내지 0.15mole/ℓ농도의 포스페이트 완충액으로 용출시켜, 측정된 용출분획중에서 단일의 명백한 피크(peak)로서 재조합 사람-인터페론을 수득함을 특징으로 하는 재조합 사람-인터페론의 크로마토그래피적 정제방법.
  2. 제1항에 있어서, 단계 a)에서 규토질 매트릭스가 조절된 다공성 유리물질 (CPG(R))그룹중에서 선택된 방법.
  3. 제2항에 있어서, CPG가 CPG(R)500인 방법.
  4. 제1항에 있어서, 단계 a)에서 아세트산 농도가 4 내지 7밀리몰/ℓ인 방법.
  5. 제4항에 있어서, 아세트산 농도가 5밀리몰/ℓ인 방법.
  6. 제1항에 있어서, 단계 b)에서 양이온 교환 매트릭스가 CM-Sepharose(R),CM-Sephadex(R),SP-Sepharose(R),SP-Sephadex(R),또는 CM-ACCEL(R)중에서 선택되는 방법.
  7. 제6항에 있어서, 양이온 교환 매트릭스가 CM-Sepharose-CL-6B인 방법.
  8. 제1항에 있어서, 단계 b)에서 재조합 사람-인터페론의 적재 및 용출 유속이 0.05 내지 0.1㎝/분인 방법.
  9. 제1항에 있어서, 단계 b)에서 아세테이트 완충액의 pH가 3.5 내지 4.5인 방법.
  10. 제9항에 있어서, 아세테이트 완충액의 pH가 4.0인 방법.
  11. 제1항에 있어서, 포스페이트 완충액의 농도가 0.09 내지 0.11몰/ℓ인 방법.
  12. 활성제로서 제1항의 방법에 따라 수득한 치료적 유효량의 재조합 사람-인터페론을 함유함을 특징으로 하는 면역조절제로서 사람의 바이러스 감염 및 중앙을 치료하기 위한 약제학적 조성물.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019910004187A 1990-03-16 1991-03-16 재조합 사람 β-인터페론의 정제방법 KR910016928A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IT19699A IT1240612B (it) 1990-03-16 1990-03-16 Procedimento di purificazione del b-interferone umano ricombinante
IT19699A/90 1990-03-16

Publications (1)

Publication Number Publication Date
KR910016928A true KR910016928A (ko) 1991-11-05

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EP (1) EP0446850B1 (ko)
JP (1) JPH0568583A (ko)
KR (1) KR910016928A (ko)
AT (1) ATE133421T1 (ko)
DE (1) DE69116582D1 (ko)
IT (1) IT1240612B (ko)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0551535A1 (en) * 1992-01-13 1993-07-21 SCLAVO S.p.A. Process for the purification of recombinant human beta interferon, beta interferon thus purified, and pharmaceutical compositions which contain them
KR100524872B1 (ko) 2003-12-04 2005-11-01 씨제이 주식회사 인터페론 베타의 정제방법
DE102009032179A1 (de) 2009-07-07 2011-01-13 Biogenerix Ag Verfahren zur Reinigung von Interferon beta

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL67896A (en) * 1983-02-13 1987-03-31 Yeda Res & Dev Two biologically active human gama interferon subtypes,purification thereof and pharmaceutical compositions containing them
US4908432A (en) * 1987-01-09 1990-03-13 New York University Novel polypeptide having gamma-interferon activity
IT1222427B (it) * 1987-07-31 1990-09-05 Sclavo Spa Procedimento per la purificazione di interferone

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DE69116582D1 (de) 1996-03-07
IT9019699A1 (it) 1991-09-16
IT9019699A0 (it) 1990-03-16
ATE133421T1 (de) 1996-02-15
EP0446850B1 (en) 1996-01-24
EP0446850A3 (en) 1991-11-06
JPH0568583A (ja) 1993-03-23
IT1240612B (it) 1993-12-17
EP0446850A2 (en) 1991-09-18

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