KR900001853A - 변형된 인간 psti - Google Patents

변형된 인간 psti Download PDF

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Publication number
KR900001853A
KR900001853A KR1019890010227A KR890010227A KR900001853A KR 900001853 A KR900001853 A KR 900001853A KR 1019890010227 A KR1019890010227 A KR 1019890010227A KR 890010227 A KR890010227 A KR 890010227A KR 900001853 A KR900001853 A KR 900001853A
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South Korea
Prior art keywords
human psti
modified human
psti
amino acid
acid sequence
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KR1019890010227A
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English (en)
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KR960015745B1 (ko
Inventor
노브오 요시다
노리히사 기구찌
마사루 신
데라오까히로시
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요시가가 이찌오
시오노기 세이야꾸 가부시기 가이샤
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Publication of KR900001853A publication Critical patent/KR900001853A/ko
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Publication of KR960015745B1 publication Critical patent/KR960015745B1/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
    • C07K14/8135—Kazal type inhibitors, e.g. pancreatic secretory inhibitor, ovomucoid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/827—Proteins from mammals or birds
    • Y10S530/843—Digestive system
    • Y10S530/845—Pancreas

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

내용 없음

Description

변형된 인간 PSTI
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 본 발명의 변형된 인간 PSTI Gln(42)-PSTI의 아미노산 서열과 이를 암호화하는 DNA를 나타낸 도면이고,
제2도는 본 발명의 변형된 인간 PSTI Ser(rr)-PSTI의 아미노산 서열과 이를암호화하는 DNA를 나타낸 도면이고,
제3도는 원래의 인간 PSTI 및 본 발명의 변형된 3종의 인간 PSTI(Ser(44)-PSTI,Thr(43)-PSTI 및 Gln(42)-PSTI)를 pH 7.0에서 트립신 처리시의 안정성에 대한 비교치를 나타내 그래프이다.

Claims (4)

  1. 원래의 인간 PSTI의 아미노산 서열의 N-말단으로부터 42번째 및/또는 44번째 위치의 알기닌을 글루타민 및/또는 세린으로 치환한 변형된 인간 PSTI.
  2. 제1항에 따른 변형된 인간 PSTI를 암호화하는 DNA서열.
  3. 원래의 인간 PSTI의 아미노산 서열의 N-말단으로부터 42번째 또는 44번째 위치의 알기닌을 각기 글루타민 또는 세린으로 치환한 변형된 인간 PSTI.
  4. 제3항에 따른 변형된 인간 PSTI를 암호화하는 DNA서열.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019890010227A 1988-07-19 1989-07-19 변형된 인간-psti KR960015745B1 (ko)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP18131688 1988-07-19
JP88-181316 1988-07-19
JP63-181316 1988-07-19
JP88-255580 1988-10-11
JP63255580A JPH07102137B2 (ja) 1988-07-19 1988-10-11 修飾ヒトｐｓｔｉ

Publications (2)

Publication Number Publication Date
KR900001853A true KR900001853A (ko) 1990-02-27
KR960015745B1 KR960015745B1 (ko) 1996-11-20

Family

ID=26500552

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019890010227A KR960015745B1 (ko) 1988-07-19 1989-07-19 변형된 인간-psti

Country Status (8)

Country Link
US (1) US5122594A (ko)
EP (1) EP0352089B1 (ko)
JP (1) JPH07102137B2 (ko)
KR (1) KR960015745B1 (ko)
AU (1) AU612865B2 (ko)
CA (1) CA1339875C (ko)
DE (1) DE68913090T2 (ko)
ES (1) ES2062006T3 (ko)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5591603A (en) * 1987-08-28 1997-01-07 Novo Nordisk A/S Process for preparing aprotinin and aprotinin analogs in yeast cells
US5231010A (en) * 1989-09-13 1993-07-27 Bayer Aktiengesellschaft Recombinant aprotinin variants genetically engineered process for the microbial preparation of homogeneously processed aprotinin variants and the therapeutic use thereof
JPH0751066B2 (ja) * 1990-09-14 1995-06-05 重三 鵜高 プロテアーゼインヒビター遺伝子及び該遺伝子を保有する微生物並びに該微生物を用いるプロテアーゼインヒビターの製造法
IL104314A0 (en) * 1992-01-07 1993-05-13 Novo Nordisk As Human kunitz-type protease inhibitor and variants thereof,their production and pharmaceutical compositions containing them
JP3626982B2 (ja) * 1997-08-29 2005-03-09 アークレイ株式会社 タンパク質分解酵素阻害物質の測定方法およびそれに用いる測定キット
US6087558A (en) * 1998-07-22 2000-07-11 Prodigene, Inc. Commercial production of proteases in plants
EP2109457B1 (en) * 2007-02-12 2016-01-06 CSL Behring GmbH Therapeutic application of kazal-type serine protease inhibitors

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3786870T2 (de) * 1986-10-14 1993-11-11 Shionogi Seiyaku Kk Methode zur Herstellung von Proteinen.
JPS63267289A (ja) * 1986-10-14 1988-11-04 Shionogi & Co Ltd 蛋白質の新規製法
WO1988003171A1 (en) * 1986-10-30 1988-05-05 Synergen Biologicals, Inc. Human pancreatic secretory trypsin inhibitors produced by recombinant dna methods and processes for the production of same
GB2199582A (en) * 1987-01-07 1988-07-13 Bayer Ag Analogues of pancreatic secretory trypsin inhibitor

Also Published As

Publication number Publication date
JPH07102137B2 (ja) 1995-11-08
CA1339875C (en) 1998-05-19
JPH02150282A (ja) 1990-06-08
DE68913090T2 (de) 1994-05-26
KR960015745B1 (ko) 1996-11-20
ES2062006T3 (es) 1994-12-16
EP0352089B1 (en) 1994-02-16
AU612865B2 (en) 1991-07-18
EP0352089A3 (en) 1990-08-16
AU3797889A (en) 1990-01-25
US5122594A (en) 1992-06-16
EP0352089A2 (en) 1990-01-24
DE68913090D1 (de) 1994-03-24

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