KR890008319A - 재조합된 단백질의 발현세포 배양법 - Google Patents

재조합된 단백질의 발현세포 배양법 Download PDF

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KR890008319A
KR890008319A KR1019880014719A KR880014719A KR890008319A KR 890008319 A KR890008319 A KR 890008319A KR 1019880014719 A KR1019880014719 A KR 1019880014719A KR 880014719 A KR880014719 A KR 880014719A KR 890008319 A KR890008319 A KR 890008319A
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interleukin
culture method
polypeptide
coli
medium
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KR1019880014719A
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마사끼 다까노
다까시 가모가시라
요시까쯔 히라이
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오오쓰가 아끼히꼬
오오쓰까세이야꾸 가부시끼가이샤
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Publication of KR890008319A publication Critical patent/KR890008319A/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
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  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
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  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
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  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
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  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
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  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

내용 없음

Description

재조합된 단백질의 발현세포 배양법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제 1 도는 실시예 2 에 따라 수득된 원하는 폴리펩타이드를 사용하여 수행한 액체크로마토그래피 분석을 나타낸 도이다.

Claims (11)

  1. 폴리펨타이드의 유전자가 삽입된 세포질 발현 벡터로 형질전환된 숙주세포를 배지중에서, 상기 배지중의 용존산소의 양을 포화농도의 약 20％ 이하로 제한해서 배양하여 발현된 폴리펩타이드의 시작 코든에 해당되는 Met 이 제거된 형태의 폴리펩타이드를 수득함을 특징으로 하는 재조합된 단백질의 발현 세포배양법.
  2. 제 1 항에 있어서, 폴리펩타이드가 림포킨인 배양법.
  3. 제 1항에 있어서, 폴리펩타이드가 인터루킨-1, 인터루킨-2. 인터루킨-3, 인터루킨-4 인터루킨-5, 인터루킨 -6, 콜로니 자극인자, 종양 괴사 인자, 또는 림포톡신인 배양법.
  4. 제1항에 있어서, 숙주세포가 이. 콜리(E.coli), 바실러스 섭틸리스(Bocillus subtilis), 디플로코커스 뉴모니아에(Diplococcus pneumoniae), 효모 또는 악티노마이세테스(actinomycetes)인 배양법.
  5. 제 1 항에 있어서, 숙주세포가 이.콜리인 배양법.
  6. 제 1 항에 있어서, 폴리펩타이드가 인터루킨-1이고 숙주세포가 이.콜리인 배양법
  7. 제 6 항에 있어서, 인터루킨-1이 인터루킨-1 α또는 인터루킨-1β 배양법.
  8. 제 1 항에 있어서, 폴리펩타이드의 유전자의 프로모터가 λPL,λPR,trp, lacC, tufB, recA 또는 Ipp인 배양법.
  9. 제 8 항에 있어서. 폴리펩타이드의 유전자의 프로모터가 trp인 배양법
  10. 제 1 항에 있어서, 배지중의 용존 산소의 양이 포화농도의 약 0내지 약5％인 배양법.
  11. 제 1 항에 있어서, 배지중의 용존 산소의 양이 대수적 중식기에서 조절되는 배양법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019880014719A 1987-11-09 1988-11-09 재조합된 단백질의 발현세포 배양법 KR890008319A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP62-283777 1987-11-09
JP28377787 1987-11-09

Publications (1)

Publication Number Publication Date
KR890008319A true KR890008319A (ko) 1989-07-10

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ID=17669989

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Application Number Title Priority Date Filing Date
KR1019880014719A KR890008319A (ko) 1987-11-09 1988-11-09 재조합된 단백질의 발현세포 배양법

Country Status (4)

Country Link
EP (1) EP0315950A3 (ko)
KR (1) KR890008319A (ko)
CN (1) CN1033837A (ko)
DK (1) DK623188A (ko)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5573930A (en) * 1985-02-05 1996-11-12 Cetus Oncology Corporation DNA encoding various forms of colony stimulating factor-1
US6117422A (en) * 1985-02-05 2000-09-12 Chiron Corporation N∇2-CSF-1(long form) and carboxy truncated fragments thereof
BG52073B2 (en) * 1990-01-24 1996-04-30 Inst Molekuljarna Biolog Method for the preparation of recombinant human noncystein -interferon, free of n-end methionine
EP0750039A1 (en) * 1995-06-20 1996-12-27 Boehringer Mannheim Gmbh Immunoregulatory protein LST-1
US6338950B2 (en) 1995-06-20 2002-01-15 Roche Diagnostics Gmbh DNA encoding LST-1 protein and process for recombinant production of LST-1 protein
EP1306430A4 (en) * 2000-07-31 2004-09-08 Takeda Chemical Industries Ltd METHOD FOR PRODUCING A RECOMBINANT PROTEIN

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4656132A (en) * 1984-03-28 1987-04-07 Cetus Corporation Method of improving the yield of heterologous protein produced by cultivating recombinant bacteria
ATE81675T1 (de) * 1985-04-30 1992-11-15 Takeda Chemical Industries Ltd Produktion eines proteins.
DE3532134A1 (de) * 1985-09-10 1987-03-12 Basf Ag Verfahren zur herstellung von proteinen bestimmter n-terminaler struktur mit hilfe von prokaryonten

Also Published As

Publication number Publication date
EP0315950A3 (en) 1990-05-23
DK623188D0 (da) 1988-11-08
CN1033837A (zh) 1989-07-12
DK623188A (da) 1989-05-10
EP0315950A2 (en) 1989-05-17

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