KR890008319A - Expression cell culture method of recombinant protein - Google Patents

Expression cell culture method of recombinant protein Download PDF

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Publication number
KR890008319A
KR890008319A KR1019880014719A KR880014719A KR890008319A KR 890008319 A KR890008319 A KR 890008319A KR 1019880014719 A KR1019880014719 A KR 1019880014719A KR 880014719 A KR880014719 A KR 880014719A KR 890008319 A KR890008319 A KR 890008319A
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South Korea
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interleukin
culture method
polypeptide
coli
medium
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KR1019880014719A
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Korean (ko)
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마사끼 다까노
다까시 가모가시라
요시까쯔 히라이
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오오쓰가 아끼히꼬
오오쓰까세이야꾸 가부시끼가이샤
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Publication of KR890008319A publication Critical patent/KR890008319A/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
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  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

내용 없음No content

Description

재조합된 단백질의 발현세포 배양법Expression cell culture method of recombinant protein

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제 1 도는 실시예 2 에 따라 수득된 원하는 폴리펩타이드를 사용하여 수행한 액체크로마토그래피 분석을 나타낸 도이다.FIG. 1 shows a liquid chromatography analysis performed using the desired polypeptide obtained according to Example 2. FIG.

Claims (11)

폴리펨타이드의 유전자가 삽입된 세포질 발현 벡터로 형질전환된 숙주세포를 배지중에서, 상기 배지중의 용존산소의 양을 포화농도의 약 20％ 이하로 제한해서 배양하여 발현된 폴리펩타이드의 시작 코든에 해당되는 Met 이 제거된 형태의 폴리펩타이드를 수득함을 특징으로 하는 재조합된 단백질의 발현 세포배양법.The host cells transformed with the cytoplasmic expression vector into which the polyfemide gene was inserted were cultured in a medium by limiting the amount of dissolved oxygen in the medium to about 20% or less of the saturation concentration. A cell culture method for expressing a recombinant protein, characterized by obtaining a polypeptide in which a corresponding Met is removed. 제 1 항에 있어서, 폴리펩타이드가 림포킨인 배양법.The culture method of claim 1, wherein the polypeptide is lymphokine. 제 1항에 있어서, 폴리펩타이드가 인터루킨-1, 인터루킨-2. 인터루킨-3, 인터루킨-4 인터루킨-5, 인터루킨 -6, 콜로니 자극인자, 종양 괴사 인자, 또는 림포톡신인 배양법.The method of claim 1, wherein the polypeptide is interleukin-1, interleukin-2. Interleukin-3, Interleukin-4 Interleukin-5, Interleukin-6, Colony Stimulator, Tumor Necrosis Factor, or Lymphotoxin. 제1항에 있어서, 숙주세포가 이. 콜리(E.coli), 바실러스 섭틸리스(Bocillus subtilis), 디플로코커스 뉴모니아에(Diplococcus pneumoniae), 효모 또는 악티노마이세테스(actinomycetes)인 배양법.The method of claim 1, wherein the host cell is E. coli. Culture method of E. coli, Bacillus subtilis, Dipolococcus pneumoniae, yeast or actinomycetes. 제 1 항에 있어서, 숙주세포가 이.콜리인 배양법.The culture method according to claim 1, wherein the host cell is E. coli. 제 1 항에 있어서, 폴리펩타이드가 인터루킨-1이고 숙주세포가 이.콜리인 배양법The culture method of claim 1, wherein the polypeptide is Interleukin-1 and the host cell is E. coli. 제 6 항에 있어서, 인터루킨-1이 인터루킨-1 α또는 인터루킨-1β 배양법.7. The method of claim 6, wherein the interleukin-1 is interleukin-1 a or interleukin-1β culture. 제 1 항에 있어서, 폴리펩타이드의 유전자의 프로모터가 λPL,λPR,trp, lacC, tufB, recA 또는 Ipp인 배양법.The culture method according to claim 1, wherein the promoter of the gene of the polypeptide is λP L, λP R, trp, lacC, tufB, recA or Ipp. 제 8 항에 있어서. 폴리펩타이드의 유전자의 프로모터가 trp인 배양법The method of claim 8. Culture method of trp promoter of gene of polypeptide 제 1 항에 있어서, 배지중의 용존 산소의 양이 포화농도의 약 0내지 약5％인 배양법.The culture method according to claim 1, wherein the amount of dissolved oxygen in the medium is about 0 to about 5% of the saturation concentration. 제 1 항에 있어서, 배지중의 용존 산소의 양이 대수적 중식기에서 조절되는 배양법.The culture method according to claim 1, wherein the amount of dissolved oxygen in the medium is controlled in an algebraic lunch. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019880014719A 1987-11-09 1988-11-09 Expression cell culture method of recombinant protein KR890008319A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP28377787 1987-11-09
JP62-283777 1987-11-09

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KR890008319A true KR890008319A (en) 1989-07-10

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KR1019880014719A KR890008319A (en) 1987-11-09 1988-11-09 Expression cell culture method of recombinant protein

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EP (1) EP0315950A3 (en)
KR (1) KR890008319A (en)
CN (1) CN1033837A (en)
DK (1) DK623188A (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5573930A (en) * 1985-02-05 1996-11-12 Cetus Oncology Corporation DNA encoding various forms of colony stimulating factor-1
US6103224A (en) * 1985-02-05 2000-08-15 Chiron Corporation N∇2 CSF-1 (short form) and carboxy truncated fragments thereof
BG52073B2 (en) * 1990-01-24 1996-04-30 Inst Molekuljarna Biolog Method for the preparation of recombinant human noncystein -interferon, free of n-end methionine
EP0750039A1 (en) * 1995-06-20 1996-12-27 Boehringer Mannheim Gmbh Immunoregulatory protein LST-1
ATE346920T1 (en) * 1995-06-20 2006-12-15 Roche Diagnostics Gmbh NEW IMMUNO-REGULATORY PROTEIN LST-1
CA2417572A1 (en) * 2000-07-31 2003-01-28 Takeda Chemical Industries, Ltd. Process for producing recombined protein

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4656132A (en) * 1984-03-28 1987-04-07 Cetus Corporation Method of improving the yield of heterologous protein produced by cultivating recombinant bacteria
IE58766B1 (en) * 1985-04-30 1993-11-03 Takeda Chemical Industries Ltd Production of protein
DE3532134A1 (en) * 1985-09-10 1987-03-12 Basf Ag METHOD FOR PRODUCING PROTEINS OF SPECIFIC N-TERMINAL STRUCTURE WITH THE AID OF PROKARYONTS

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DK623188D0 (en) 1988-11-08
CN1033837A (en) 1989-07-12
DK623188A (en) 1989-05-10
EP0315950A3 (en) 1990-05-23
EP0315950A2 (en) 1989-05-17

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