KR880700811A - 잡종형성에 의하여 세균 및 기타 살아있는 유기체를 검출하기 위한 올리고 핵삼염 소식자 및 검출 방법 - Google Patents

잡종형성에 의하여 세균 및 기타 살아있는 유기체를 검출하기 위한 올리고 핵삼염 소식자 및 검출 방법

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KR880700811A
KR880700811A KR870700808A KR870700808A KR880700811A KR 880700811 A KR880700811 A KR 880700811A KR 870700808 A KR870700808 A KR 870700808A KR 870700808 A KR870700808 A KR 870700808A KR 880700811 A KR880700811 A KR 880700811A
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bacteria
dna fragments
residue
electrophoresis
chromatography
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KR870700808A
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KR950013772B1 (ko
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말리에르삘리쁘
빠뜨리끄 그리몽
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원본미기재
엥스띠뛰 빠스뛰르
엥스띠뛰 나쇼날 드 라 상뜨 에 드 라 르셰르셰 메디깔
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)

Abstract

내용 없음

Description

잡종형성에 의하여 세균 및 기타 살아있는 유기체를 검출하기 위한 올리고핵삼염 소식자 및 검출 방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (10)

  1. 시료중의 세균을 검출하거나, 또는 전기 영동 또는 크로마토그래피에 의하여 분리된 DNA소편들 중 세균의 16S RAN에 대하여 암호화된 유전자를 가진 DNA소편의 위치를 확인하기 위한 것으로서, (5')d-AAG GAG GTG ATC CAG(3') [여기서, (5')및 (3')은 폴리머의 5' 및 3'말단을 나타내며, 문자는 다음의 핵산염 잔기를 나타냄, 즉, A아데닐 잔기, C시티딜 잔기, T티미딜 잔기, G구아니딜 잔기]의 서열을 갖는 펜타데카데옥시리보핵산염, 또는 (5')d-CTG GAT CAC CTC CTT(3')의 보족 서열을 갖는 펜타데카데옥시리보핵산염으로 되는 것을 특징으로 하는 소식자.
  2. 시료중의 세균을 검출하거나, 또는 전기영동 또는 크로마토그래피에 의하여 분리된 DNA소면들 중 세균의 16S RNA에 대하여 암호화된 유전자를 가진 DNA소편의 위치를 확인하기 위한 것으로서, (3')ACCTAGTGGAGGAAA(5')의 서열 또는 그의 대응하는 보족 서열로 되는 것을 특징으로 하는 소식자.
  3. 시료중의 세균을 검출하거나, 또는 전기 영동 또는 크로마토그래피에 의하여 분리된 DNA소편들 중 세균의 16S RNA에 대하여 암호화된 유전자를 가진 DNA소편의 위치를 확인하기 위한 것으로서, 다음 서열군, (5')d-AAGGAGGT(3'), (5')d-AAAGGAGGT(3'), (5')d-AAGGAGGTG(3'), (5')d-AAGGAGGTGA(3'), (5')d-AAGGAGGTGAT(3'), (5')d-AAGGAGGTGATC(3'), (5')d-AAGGAGGTGATCC(3') (5')d-AAGGAGGTGATCCA(3'), 또는 이들의 대응하는 보족 서열로 되는 군 중에서 선택되는 것을 특징으로 하는 소식자.
  4. 시료중의 진핵 생물 또는 원핵 생물(세균 포함) 검출하거나, 또는 전기영동 또는 크로마토그래피에 의하여 분리된 DNA소편들 중 세균의 16S RNA에 대하여 암호화된 유전자를 가진 DNA소편의 위치를 확인하기 위한 것으로서, (5')d-ACC TTG TTA CGACTT(3'), [여기서, (5')및 (3')은 플리머의 5'및 3'말단을 나타내며, 문자는 다음의 핵산염 잔기를 나타냄, 즉, A아데닐 잔기, C시티딜 잔기, T티미딜 잔기, G구아니딜 잔기]의 서열을 갖는 펜타데카데옥시리보핵산염, 또는 (5')d-AAG TCG TAA CAAGGT(3')의 보족 서열을 갖는 펜타데카데옥시리보핵산염으로 되는 것을 특징으로 하는 소식자.
  5. 시료 중의 원시 세균을 검출하거나, 또는 전기영동 또는 크로마토그래피에 의하여 분리된 DNA소편들 중 원시 세균의 16S RNA에 대하여 암호화된 유전자를 가진 DNA소편의 위치를 확인하기 위한 것으로서, 다음 서열 AAGCTGCTCR'ACCCCGCG (여기서, R'는 T또는 C임)중에 함유된 적어도 8개, 최대 18개의 핵산염 서열 또는 그의 보족 서열로 되는 것을 특징으로 하는 소식자.
  6. 제1항 내지 5항 중 어느 하나의 항에 있어서, 상기 서열 중 하나의 서열을 갖는 올리고핵산염 소식자에 방사성 표지가 부착되거나 (특히, 임의의 성상을 갖는 1-2개의 방사성 핵산염이 소식자의 양말단 중 하나 또는 양 말단 모두에 부착됨)효소, 리간드, 발광 또는 형광 표지가 부착된 것을 특징으로 하는 소식자.
  7. 세균의 핵산을 얻고 한가닥으로 만든 후, 이것을 잡종 형성에 유리한 조건 하에서 제1항 내지 3항 중 어느 하나의 항에 의한 소식자와 접촉시키고, 형성된 임의의 잡종을 검출하는 것을 특징으로 하는 시료 중의 세균 검출 방법.
  8. 세포의 핵산을 얻고 한가닥으로 만든 후, 이것을 잡종 형성에 유리한 조건 하에서 제4항에 의한 소식지와 접촉시키고, 형성된 임의의 잡종을 검출하는 것을 특징으로 하는 시료 중 진핵 생물 또는 원핵 생물(세균 포함)세포의 검출 방법.
  9. 원시 세균의 핵산을 얻고 한가닥으로 만든 후, 이것을 잡종 형성에 유리한 조건 하에서 제5항에 의한 소식자와 접촉시키고, 형성된 임의의 잡종을 검출하는 것을 특징으로 하는 시료 중 원시 세균의 검출 방법.
  10. 전기 영동 또는 크로마토그래피에 의하여 분리 및 변성시킨 DNA소편들을 잡종 형성을 허용하는 조건 하에서 제1항 내지 5항 중 어느 하나의 항에 의한 조성물과 접촉시키고, 형성된 임의의 잡종을 검출하는 것을 특징으로 하는 이들 DNA소편 중 16S 서열의 RNA에 대하여 암호화된 유전자를 가진 DNA소편의 위치를 확인하는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019870700808A 1986-04-04 1987-04-03 혼성화에 의하여 세균 및 기타 생 유기체를 검출하기 위한 올리고뉴클레오티드 프로브의 제조 방법 KR950013772B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR8604914A FR2596774B1 (fr) 1986-04-04 1986-04-04 Sondes oligonucleotidiques et procedes pour la detection par hybridation des acides nucleiques de bacteries et autres etres vivants
FR8604914 1986-04-04
PCT/FR1987/000108 WO1987005907A1 (fr) 1986-04-04 1987-04-03 Sondes oligonucleotidiques et procedes pour la detection par hybridation des acides nucleiques de bacteries et autres etres vivants

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KR880700811A true KR880700811A (ko) 1988-04-12
KR950013772B1 KR950013772B1 (ko) 1995-11-15

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KR1019870700808A KR950013772B1 (ko) 1986-04-04 1987-04-03 혼성화에 의하여 세균 및 기타 생 유기체를 검출하기 위한 올리고뉴클레오티드 프로브의 제조 방법

Country Status (14)

Country Link
US (1) US5407797A (ko)
EP (1) EP0245129B1 (ko)
JP (1) JP2662556B2 (ko)
KR (1) KR950013772B1 (ko)
AT (1) ATE72567T1 (ko)
AU (1) AU612571B2 (ko)
DE (1) DE3776669D1 (ko)
DK (1) DK453887A (ko)
EG (1) EG18267A (ko)
ES (1) ES2039469T3 (ko)
FI (1) FI89185C (ko)
FR (1) FR2596774B1 (ko)
GR (1) GR3003796T3 (ko)
WO (1) WO1987005907A1 (ko)

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Also Published As

Publication number Publication date
EP0245129A1 (fr) 1987-11-11
KR950013772B1 (ko) 1995-11-15
FI873774A (fi) 1987-10-05
EP0245129B1 (fr) 1992-02-12
ES2039469T3 (es) 1993-10-01
FI89185B (fi) 1993-05-14
FI89185C (fi) 1993-08-25
GR3003796T3 (ko) 1993-03-16
AU7232587A (en) 1987-10-20
US5407797A (en) 1995-04-18
JPH01500001A (ja) 1989-01-12
EG18267A (en) 1992-12-30
DK453887D0 (da) 1987-08-28
WO1987005907A1 (fr) 1987-10-08
FR2596774B1 (fr) 1988-07-22
JP2662556B2 (ja) 1997-10-15
FR2596774A1 (fr) 1987-10-09
AU612571B2 (en) 1991-07-18
DK453887A (da) 1987-08-28
ATE72567T1 (de) 1992-02-15
FI873774A0 (fi) 1987-08-31
DE3776669D1 (de) 1992-03-26

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