KR20250162849A - 신경 오르가노이드의 제조 방법 및 그 이용 - Google Patents

신경 오르가노이드의 제조 방법 및 그 이용

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Publication number
KR20250162849A
KR20250162849A KR1020257034462A KR20257034462A KR20250162849A KR 20250162849 A KR20250162849 A KR 20250162849A KR 1020257034462 A KR1020257034462 A KR 1020257034462A KR 20257034462 A KR20257034462 A KR 20257034462A KR 20250162849 A KR20250162849 A KR 20250162849A
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South Korea
Prior art keywords
cells
organoids
neural
cell
culture
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
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KR1020257034462A
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English (en)
Korean (ko)
Inventor
준 다카하시
붐페이 사마타
다이스케 도이
유 이마이즈미
고시 구노
Original Assignee
고쿠리츠 다이가쿠 호진 교토 다이가쿠
도소 가부시키가이샤
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Application filed by 고쿠리츠 다이가쿠 호진 교토 다이가쿠, 도소 가부시키가이샤 filed Critical 고쿠리츠 다이가쿠 호진 교토 다이가쿠
Publication of KR20250162849A publication Critical patent/KR20250162849A/ko
Pending legal-status Critical Current

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/30—Nerves; Brain; Eyes; Corneal cells; Cerebrospinal fluid; Neuronal stem cells; Neuronal precursor cells; Glial cells; Oligodendrocytes; Schwann cells; Astroglia; Astrocytes; Choroid plexus; Spinal cord tissue
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/3641—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the site of application in the body
    • A61L27/3675—Nerve tissue, e.g. brain, spinal cord, nerves, dura mater
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
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  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
KR1020257034462A 2023-03-20 2024-03-19 신경 오르가노이드의 제조 방법 및 그 이용 Pending KR20250162849A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2023044570 2023-03-20
JPJP-P-2023-044570 2023-03-20
PCT/JP2024/010698 WO2024195789A1 (ja) 2023-03-20 2024-03-19 神経オルガノイドの製造方法およびその利用

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KR20250162849A true KR20250162849A (ko) 2025-11-19

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KR1020257034462A Pending KR20250162849A (ko) 2023-03-20 2024-03-19 신경 오르가노이드의 제조 방법 및 그 이용

Country Status (5)

Country Link
EP (1) EP4685230A1 (https=)
JP (1) JPWO2024195789A1 (https=)
KR (1) KR20250162849A (https=)
CN (1) CN120958118A (https=)
WO (1) WO2024195789A1 (https=)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20150076388A (ko) 2013-12-26 2015-07-07 두산인프라코어 주식회사 건설기계용 투스 어댑터의 용접 방법
JP2021158960A (ja) 2020-03-31 2021-10-11 東ソー株式会社 多能性幹細胞から外胚葉系細胞を分化誘導する方法及び製造方法

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US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
CA2277278A1 (en) 1997-01-10 1998-07-16 Life Technologies, Inc. Embryonic stem cell serum replacement
US20020103360A1 (en) 1998-09-01 2002-08-01 Yang Pan Novel protein related to melanoma-inhibiting protein and uses thereof
US6280718B1 (en) 1999-11-08 2001-08-28 Wisconsin Alumni Reasearch Foundation Hematopoietic differentiation of human pluripotent embryonic stem cells
WO2002101057A1 (fr) 2001-06-08 2002-12-19 Dnavec Research Inc. Transfert genique dans des cellules souches embryonnaires de primate a l'aide d'un virus de l'immunodeficience simienne de pseudo type vsv-g utilise comme vecteur
JP6019771B2 (ja) * 2012-06-01 2016-11-02 大日本印刷株式会社 細胞培養方法及び細胞培養容器
JP6363401B2 (ja) * 2013-06-10 2018-07-25 大日本印刷株式会社 人工多能性幹細胞の分化誘導方法
ES2732730T3 (es) 2013-11-22 2019-11-25 Riken Método para producir telencéfalo o tejido progenitor del mismo
JP6900217B2 (ja) * 2017-03-27 2021-07-07 一般財団法人電力中央研究所 培養細胞供試体の作製方法
WO2019035436A1 (ja) * 2017-08-16 2019-02-21 東ソー株式会社 多能性幹細胞の培養基材及び多能性幹細胞の製造方法
US12180442B2 (en) 2017-11-30 2024-12-31 Public University Corporation Yokohama City University Method for aggregating cell mass and device for aggregating cell mass
JP7366332B2 (ja) 2019-06-05 2023-10-23 公立大学法人奈良県立医科大学 脳幹オルガノイドの作製方法
JP7725821B2 (ja) * 2020-03-23 2025-08-20 株式会社リコー 神経細胞が配置された基板の製造方法
JP7755289B2 (ja) 2021-09-17 2025-10-16 ユニソック合同会社 マッチング支援システム、マッチング支援プログラム及びマッチング支援方法

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Publication number Priority date Publication date Assignee Title
KR20150076388A (ko) 2013-12-26 2015-07-07 두산인프라코어 주식회사 건설기계용 투스 어댑터의 용접 방법
JP2021158960A (ja) 2020-03-31 2021-10-11 東ソー株式会社 多能性幹細胞から外胚葉系細胞を分化誘導する方法及び製造方法

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Kadoshima T, et al., Proc Natl Acad Sci U S A. 110(50) : 20284- 20289 (2013)
Sakaguchi H, et al., Stem Cell Reports, 10 ; 13(3) : 458-473 (2019)

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CN120958118A (zh) 2025-11-14
EP4685230A1 (en) 2026-01-28
WO2024195789A1 (ja) 2024-09-26
JPWO2024195789A1 (https=) 2024-09-26

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