KR20250151452A - 단백질의 분비 생산법 - Google Patents

단백질의 분비 생산법

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Publication number
KR20250151452A
KR20250151452A KR1020257030808A KR20257030808A KR20250151452A KR 20250151452 A KR20250151452 A KR 20250151452A KR 1020257030808 A KR1020257030808 A KR 1020257030808A KR 20257030808 A KR20257030808 A KR 20257030808A KR 20250151452 A KR20250151452 A KR 20250151452A
Authority
KR
South Korea
Prior art keywords
protein
gene
amino acid
acid sequence
signal peptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
KR1020257030808A
Other languages
English (en)
Korean (ko)
Inventor
유미 나가세
요시히코 마츠다
Original Assignee
아지노모토 가부시키가이샤
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 아지노모토 가부시키가이샤 filed Critical 아지노모토 가부시키가이샤
Publication of KR20250151452A publication Critical patent/KR20250151452A/ko
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70567—Nuclear receptors, e.g. retinoic acid receptor [RAR], RXR, nuclear orphan receptors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/104—Aminoacyltransferases (2.3.2)
    • C12N9/1044—Protein-glutamine gamma-glutamyltransferase (2.3.2.13), i.e. transglutaminase or factor XIII
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01037—Malate dehydrogenase (1.1.1.37)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/02—Aminoacyltransferases (2.3.2)
    • C12Y203/02013—Protein-glutamine gamma-glutamyltransferase (2.3.2.13), i.e. transglutaminase or factor XIII
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/10—Fusion polypeptide containing a localisation/targetting motif containing a tag for extracellular membrane crossing, e.g. TAT or VP22
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/15—Corynebacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/265—Micrococcus
    • C12R2001/28—Micrococcus glutamicus ; Corynebacterium glutamicum

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • High Energy & Nuclear Physics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
KR1020257030808A 2023-02-16 2024-02-16 단백질의 분비 생산법 Pending KR20250151452A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JPJP-P-2023-022171 2023-02-16
JP2023022171 2023-02-16
PCT/JP2024/005612 WO2024172163A1 (ja) 2023-02-16 2024-02-16 タンパク質の分泌生産法

Publications (1)

Publication Number Publication Date
KR20250151452A true KR20250151452A (ko) 2025-10-21

Family

ID=92419941

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020257030808A Pending KR20250151452A (ko) 2023-02-16 2024-02-16 단백질의 분비 생산법

Country Status (7)

Country Link
US (1) US20260071227A1 (https=)
EP (1) EP4667580A1 (https=)
JP (1) JPWO2024172163A1 (https=)
KR (1) KR20250151452A (https=)
CN (1) CN120693407A (https=)
AU (1) AU2024221604A1 (https=)
WO (1) WO2024172163A1 (https=)

Citations (12)

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US4965197A (en) 1987-06-12 1990-10-23 Massachusetts Institute Of Technology Coryneform expression and secretion system
JPH06502548A (ja) 1991-07-30 1994-03-24 オルサン 特にコリネバクテリア中で用いることのできる蛋白質の発現および分泌系
JPH11169182A (ja) 1997-12-10 1999-06-29 Mitsubishi Chemical Corp コリネ型細菌内で蛋白質を効率よく培地中へ分泌させる変異型分泌装置遺伝子
JP2003144161A (ja) 1999-07-02 2003-05-20 Ajinomoto Co Inc Ｌ−アミノ酸の製造法
JP4320769B2 (ja) 1999-09-30 2009-08-26 味の素株式会社 トランスグルタミナーゼの製造法
JP4362651B2 (ja) 2001-03-30 2009-11-11 味の素株式会社 タンパク質の分泌生産法
JP4730302B2 (ja) 2004-04-20 2011-07-20 味の素株式会社 タンパク質の製造法
JP4760121B2 (ja) 2005-05-17 2011-08-31 三菱化学株式会社 コハク酸の製造方法
WO2013062029A1 (ja) 2011-10-25 2013-05-02 味の素株式会社 タンパク質の分泌生産法
WO2013065772A1 (ja) 2011-11-02 2013-05-10 味の素株式会社 タンパク質の分泌生産法
WO2013065869A1 (en) 2011-11-02 2013-05-10 Ajinomoto Co.,Inc. Method for secretory production of protein
WO2013118544A1 (ja) 2012-02-08 2013-08-15 味の素株式会社 タンパク質の分泌生産法

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JPS5835197A (ja) 1981-08-26 1983-03-01 Kyowa Hakko Kogyo Co Ltd プラスミドｐｃｇ２
JPH01191686A (ja) 1988-01-26 1989-08-01 Mitsubishi Petrochem Co Ltd 複合プラスミド
FR2627508B1 (fr) 1988-02-22 1990-10-05 Eurolysine Procede pour l'integration d'un gene choisi sur le chromosome d'une bacterie et bacterie obtenue par ledit procede
US5185262A (en) 1988-07-27 1993-02-09 Mitsubishi Petrochemical Co., Ltd. DNA fragment containing gene which encodes the function of stabilizing plasmid in host microorganism
JP2678995B2 (ja) 1988-09-08 1997-11-19 三菱化学株式会社 トリプトフアンシンターゼの製造法
JPH02207791A (ja) 1989-02-07 1990-08-17 Ajinomoto Co Inc 微生物の形質転換法
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JPH07108228B2 (ja) 1990-10-15 1995-11-22 味の素株式会社 温度感受性プラスミド
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KR102337932B1 (ko) 2016-10-21 2021-12-13 아지노모토 가부시키가이샤 단백질의 분비 생산법
KR102335301B1 (ko) 2016-10-21 2021-12-06 아지노모토 가부시키가이샤 단백질의 분비 생산법
KR102744950B1 (ko) * 2018-04-20 2024-12-20 아지노모토 가부시키가이샤 단백질의 분비 생산법
JP2021192900A (ja) 2020-06-08 2021-12-23 京三電機株式会社 タンク清掃装置用制御装置、タンク清掃装置、タンク清掃装置用制御方法およびタンク清掃装置用制御プログラム
JP7825517B2 (ja) 2022-06-16 2026-03-06 シャープ株式会社 非接触型の温度測定装置及びそれを備えた画像形成装置
JP2025134500A (ja) 2024-03-04 2025-09-17 アイホン株式会社 電気機器

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965197A (en) 1987-06-12 1990-10-23 Massachusetts Institute Of Technology Coryneform expression and secretion system
JPH06502548A (ja) 1991-07-30 1994-03-24 オルサン 特にコリネバクテリア中で用いることのできる蛋白質の発現および分泌系
JPH11169182A (ja) 1997-12-10 1999-06-29 Mitsubishi Chemical Corp コリネ型細菌内で蛋白質を効率よく培地中へ分泌させる変異型分泌装置遺伝子
JP2003144161A (ja) 1999-07-02 2003-05-20 Ajinomoto Co Inc Ｌ−アミノ酸の製造法
JP4320769B2 (ja) 1999-09-30 2009-08-26 味の素株式会社 トランスグルタミナーゼの製造法
JP4362651B2 (ja) 2001-03-30 2009-11-11 味の素株式会社 タンパク質の分泌生産法
JP4730302B2 (ja) 2004-04-20 2011-07-20 味の素株式会社 タンパク質の製造法
JP4760121B2 (ja) 2005-05-17 2011-08-31 三菱化学株式会社 コハク酸の製造方法
WO2013062029A1 (ja) 2011-10-25 2013-05-02 味の素株式会社 タンパク質の分泌生産法
WO2013065772A1 (ja) 2011-11-02 2013-05-10 味の素株式会社 タンパク質の分泌生産法
WO2013065869A1 (en) 2011-11-02 2013-05-10 Ajinomoto Co.,Inc. Method for secretory production of protein
WO2013118544A1 (ja) 2012-02-08 2013-08-15 味の素株式会社 タンパク質の分泌生産法

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비특허문헌 1: Microbiol. rev., 57, 109-137(1993)
비특허문헌 10: J. Bacteriol., 182, 6884-6891(2000)
비특허문헌 11: Appl. Environ. Microb., 77, 3300-3310(2011)
비특허문헌 2: Biotechnol., 11, 905-910(1993)
비특허문헌 3: Biotechnol., 6, 1419-1422(1988)
비특허문헌 4: Biotechnol., 9, 976-981(1991)
비특허문헌 5: J. Bacteriol., 174, 1854-1861(1992)
비특허문헌 6: Appl. Environ. Microbiol., 61, 1610-1613(1995)
비특허문헌 7: Appl. Environ. Microbiol., 63, 4392-4400(1997)
비특허문헌 8: EMBO J., 14, 2715-2722(1995)
비특허문헌 9: J. Biol. Chem., 25;273(52), 34868-74(1998)

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JPWO2024172163A1 (https=) 2024-08-22
US20260071227A1 (en) 2026-03-12
EP4667580A1 (en) 2025-12-24
WO2024172163A1 (ja) 2024-08-22
CN120693407A (zh) 2025-09-23
AU2024221604A1 (en) 2025-09-11

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