KR20240073297A - Cosmetic composition for skin whitening containing lactobacillus rhamnosus lm1011 as active ingredient - Google Patents

Abstract

Provided is a cosmetic composition for skin whitening containing Lactobacillus rhamnosus LM1011 as an active ingredient.

Description

Cosmetic composition for skin whitening containing Lactobacillus rhamnosus LM1011 as an active ingredient {COSMETIC COMPOSITION FOR SKIN WHITENING CONTAINING LACTOBACILLUS RHAMNOSUS LM1011 AS ACTIVE INGREDIENT}

The present invention relates to a cosmetic composition for skin whitening containing Lactobacillus rhamnosus LM1011 as an active ingredient.

A person's skin color is mainly determined by the content of a pigment called melanin present in the skin. Melanin is biosynthesized by melanin-producing cells (melanocytes) present in the basal layer of the skin, and moves from the basal layer of the epidermis to the stratum corneum through the keratinization process of keratinocytes through cytoplasmic protrusions.

Melanin is produced through several oxidation processes after tyrosine, a type of amino acid, is converted into DOPA and DOPA quinone by an enzyme called tyrosinase in melanin-producing cells. This melanin absorbs ultraviolet rays and plays a role in protecting various cell tissues present inside the epidermis from damage caused by ultraviolet rays. If melanin synthesis is excessive, it not only causes spots and freckles, but also darkens the overall skin color. Therefore, inhibiting excessive melanin pigment synthesis can not only brighten skin color but also alleviate skin hyperpigmentation symptoms such as spots and freckles.

Meanwhile, Lactobacillus rhamnosus is a type of lactic acid bacterium and is known to have various effects such as intestinal regulation, antibacterial effect, prevention of atopy, and reduction of body fat. In addition, a lot of research has been conducted recently on killed lactic acid bacteria. Lipoteichoic acid, a cell wall component generated during the process of killing live bacteria, attaches to the barrier and inhibits the establishment of harmful bacteria. It is known that antibacterial components in fermented products, such as lactic acid and bacteriocin, have the ability to directly act on and suppress harmful bacteria. In addition, immunoglobulin A, an important substance in defense against infection by external bacteria, is usually produced by killed lactic acid bacteria cells absorbed into the small intestine and stimulating macrophages rather than by live lactic acid bacteria, and killed lactic acid bacteria serve as food for live bacteria. It has been revealed that killed lactic acid bacteria function as excellent prebiotics in the intestinal regulation of lactic acid bacteria.

The problem to be solved by the present invention is to provide a cosmetic composition for skin whitening containing Lactobacillus rhamnosus LM1011 as an active ingredient.

The problems to be solved by the present invention are not limited to the problems mentioned above, and other problems not mentioned can be clearly understood by those skilled in the art from the description below.

The cosmetic composition of the present invention for solving the above-mentioned problems may be characterized by containing Lactobacillus rhamnosus LM1011 (Lactobacillus rhamnosus LM1011) as an active ingredient.

A skin whitening product comprising Lactobacillus rhamnosus LM1011 (Depositor: Korea Center for Biological Resources, Accession number: KCTC12751BP, Accession date: April 18, 2019) as an active ingredient according to an embodiment of the present application. The cosmetic composition reduces the production of melanin by regulating tyrosinase activity and has a skin whitening effect, especially for hypermelanosis (ypermelanosis).

The effects of the present invention are not limited to the effects mentioned above, and other effects not mentioned will be clearly understood by those skilled in the art from the description below.

Figure 1 shows the melanin production reduction mechanism of Lactobacillus rhamnosus LM1011 (Depositor: Korea Center for Biological Resources, Accession number: KCTC12751BP, Accession date: April 18, 2019) in an embodiment of the present application. This is a schematic diagram showing .
Figure 2 is a graph showing the effect of reducing melanin production of a whitening cosmetic composition containing Lactobacillus rhamnosus LM1011 according to an example herein in the B16-F10 melanoma cell line.
Figure 3 shows the M-value over time ( This is a graph showing the change in the improvement rate of the melanin index.
Figure 4 is a graph showing changes in visual evaluation scores over time of a whitening cosmetic composition containing Lactobacillus rhamnosus LM1011 in a clinical trial for whitening effect on hypermelanosis, according to an example of the present application.
Figure 5 is a graph showing the change in the improvement rate of L-value (lightness value) over time measured using a chromameter in a clinical trial of the brightening effect for hypermelanosis in an embodiment of the present application. .

The advantages and features of the present invention and methods for achieving them will become clear by referring to the embodiments described in detail below along with the accompanying drawings. However, the present invention is not limited to the embodiments disclosed below and may be implemented in various different forms. The present embodiments are merely provided to ensure that the disclosure of the present invention is complete and to provide a general understanding of the technical field to which the present invention pertains. It is provided to fully inform the skilled person of the scope of the present invention, and the present invention is only defined by the scope of the claims.

The terminology used herein is for describing embodiments and is not intended to limit the invention. As used herein, singular forms also include plural forms, unless specifically stated otherwise in the context. As used in the specification, “comprises” and/or “comprising” does not exclude the presence or addition of one or more other elements in addition to the mentioned elements. Like reference numerals refer to like elements throughout the specification, and “and/or” includes each and every combination of one or more of the referenced elements. Although “first”, “second”, etc. are used to describe various components, these components are of course not limited by these terms. These terms are merely used to distinguish one component from another. Therefore, it goes without saying that the first component mentioned below may also be a second component within the technical spirit of the present invention.

Unless otherwise defined, all terms (including technical and scientific terms) used in this specification may be used with meanings commonly understood by those skilled in the art to which the present invention pertains. Additionally, terms defined in commonly used dictionaries are not to be interpreted ideally or excessively unless clearly specifically defined.

Throughout this specification, references to “A and/or B” mean “A or B, or A and B.”

Hereinafter, implementation examples and examples of the present application will be described in detail with reference to the attached drawings. However, the present application may not be limited to these implementations, examples, and drawings.

The first aspect of the present application is a skin whitening agent containing Lactobacillus rhamnosus LM1011 (Depositor: Korea Center for Biological Resources, Accession number: KCTC12751BP, Accession date: April 18, 2019) as an active ingredient. A cosmetic composition is provided.

In one embodiment of the present application, the cosmetic composition for skin whitening may include the Lactobacillus rhamnosus LM1011 strain, dead cells, lysates, culture products, concentrates, or extracts thereof as active ingredients, but is not limited thereto. It may not be possible. For example, the cosmetic composition for skin whitening may include heat-treated Lactobacillus rhamnosus LM1011 strain as an active ingredient, but may not be limited thereto.

In one embodiment of the present application, by heat-treating the Lactobacillus rhamnosus LM1011 strain, the dead lactic acid bacteria themselves can be utilized, and when the Lactobacillus rhamnosus LM1011 strain is applied to cosmetic products, product instability due to abnormal fermentation can be reduced, It may not be limited to this.

In one embodiment of the present application, the content of the cosmetic composition for skin whitening is not particularly limited as long as it contains the Lactobacillus rhamnosus LM1011. For example, the Lactobacillus rhamnosus LM1011 is used in about 1×10 ¹¹ cells. /mL to about 5×10 ¹¹ cells/mL, but may not be limited thereto.

In one embodiment of the present application, when the concentration of Lactobacillus rhamnosus LM1011 in the cosmetic composition for skin whitening is less than about 1×10 ¹¹ cells/mL or more than about 5×10 ¹¹ cells/mL, the skin whitening Since the effect may be reduced, the concentration of Lactobacillus rhamnosus LM1011 is about 1×10 ¹¹ cells/mL to about 5×10 ¹¹ cells/mL, about 1×10 ¹¹ cells/mL to about 4×10 ¹¹ cells/mL. mL, from about 1×10 ¹¹ cells/mL to about 3×10 ¹¹ cells/mL, from about 1×10 ¹¹ cells/mL to about 2×10 ¹¹ cells/mL, from about 2×10 ¹¹ cells/mL to about 5× It may be, but is not limited to, 10 ¹¹ cells/mL, or about 3×10 ¹¹ cells/mL to about 5×10 ¹¹ cells/mL.

In one embodiment of the present application, the Lactobacillus rhamnosus LM1011 may reduce melanin production, but may not be limited thereto. For example, the Lactobacillus rhamnosus LM1011 may reduce melanin production in the skin increased by exposure to ultraviolet rays, but may not be limited thereto.

In one embodiment of the present application, the Lactobacillus rhamnosus LM1011 may reduce melanin production by regulating tyrosinase activity, but may not be limited thereto. For example, the Lactobacillus rhamnosus LM1011 changes L-tyrosine into L-dopa and changes the L-dopa into dopaquinone to regulate the activity of tyrosinase, an enzyme that produces melanin, thereby reducing melanin in the skin. It may be to reduce production, but may not be limited thereto.

In one embodiment of the present application, the formulation of the cosmetic composition for skin whitening is not particularly limited, and for example, any one selected from skins, lotions, essences, creams, packs, foundations, and makeup bases. It may have a formulation, but may not be limited thereto.

In one embodiment of the present application, the cosmetic composition for skin whitening may contain substances arbitrarily selected according to the formulation or purpose of use of the cosmetic, but may not be limited thereto. For example, purified water, oil, surfactant, moisturizer, higher alcohol, viscosity reducing agent, chelating agent, colorant, fatty acid, antioxidant, preservative, wax, pH adjuster, fragrance, etc. may be added, but may not be limited thereto. there is.

In one embodiment of the present application, when the formulation of the cosmetic composition for skin whitening is a paste, cream or gel, the carrier ingredients include animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicon, It may include bentonite, silica, talc, or zinc oxide, but may not be limited thereto.

In one embodiment of the present application, when the formulation of the cosmetic composition for skin whitening is powder or spray, it may include lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder as a carrier ingredient, especially , in the case of a spray, it may additionally include a propellant such as chlorofluorohydrocarbon, propane/butane, or dimethyl ether, but may not be limited thereto.

In one embodiment of the present application, when the formulation of the cosmetic composition for skin whitening is a solution or emulsion, it may include a solvent, solubilizing agent, or emulsifying agent as a carrier component, for example, water, ethanol, isopropanol, It may include, but is not limited to, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic ester, polyethylene glycol, or fatty acid ester of sorbitan.

In one embodiment of the present application, when the formulation of the cosmetic composition for skin whitening is a suspension, the carrier ingredients include water, a liquid diluent such as ethanol or propylene glycol, ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and poly It may include, but is not limited to, a suspending agent such as oxyethylene sorbitan ester, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracant.

In one embodiment of the present application, when the formulation of the cosmetic composition for skin whitening is a surfactant-containing cleansing agent, the carrier ingredients include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, and imidazolinium. It may include derivatives, methyl taurate, sarcosinate, fatty acid amide ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative or ethoxylated glycerol fatty acid ester, etc. It may not be limited to this.

Hereinafter, the present invention will be described in more detail through examples of the present application, but the following examples will aid understanding of the present application.

For this purpose, the contents of the present application are not limited to the following examples, but are merely illustrative.

[Example]

1. Manufacturing and cell testing of dead lactic acid bacteria

1-1. Preparation of a cosmetic composition for skin whitening containing Lactobacillus rhamnosus LM1011

Dead cells of Lactobacillus rhamnosus LM1011 according to this example were prepared by batch culture.

The lactic acid bacteria culture was performed in a 100 L fermenter (Corbiotech) with a total volume of 60 L of culture medium, the stirring speed was maintained at 30 rpm, the temperature was maintained at 35°C to 37°C, and the pH was maintained at 5 to 6.5. After culturing, the culture medium was centrifuged at 13,000 rpm for 30 minutes to recover bacterial cells. The recovered bacterial cells were diluted twice by weight using physiological saline (0.9% NaCl), freeze-dried in a high-pressure sterilizer at 110°C for 10 minutes through the Tyndall process, and finally heat-treated dead lactic acid bacteria (Lactobacillus rhamnosus LM1011). Acquired.

1-2. Melanin production reduction effect (in vitro)

The efficacy of heat-treated Lactobacillus rhamnosus LM1011, obtained in the above example, in reducing melanin production was confirmed. The cell line used was the B16-F10 melanoma cell line, and it was inoculated into a 6-well cell culture plate at a concentration of 1 × 10 ⁴ cells/well. After culturing for 24 hours, the cell line was heat-treated with Lactobacillus rhamnosus. The concentration of LM1011 was adjusted to 0 (negative control), 1×10 ⁴ , 1×10 ⁵ , 1×10 ⁶ , 1×10 ⁷ , and 1×10 ⁸ cells/mL, respectively, and then cultured for 48 hours. To determine whether melanin production is inhibited, the amount of protein expressed in cells is measured using EX-CYTOX (EZ-3000, Daeil lab service) and the absorbance is measured at 450 nm using a multi-plate reader, and then the melanin synthesis inhibition rate is compared with that of the negative control group. In addition to the heat-treated Lactobacillus rhamnosus LM1011 strain test group, β-arbutin, a compound showing whitening function, was also compared as a positive control at 200 ug/mL.

As shown in Figure 2, heat-treated Lactobacillus rhamnosus LM1011 showed an effective melanin production reduction effect at a concentration of 1×10 ⁵ to 1×10 ⁸ cells/mL compared to the negative control group, especially at 1×10 ⁸ cells. At a concentration of /mL, it showed a melanin production reduction effect that was about 20% higher than that of β-arbutin.

2. Manufacturing and human application testing of cosmetics containing dead cells

2-1. cosmetic manufacturing

The dead Lactobacillus rhamnosus LM1011 lactic acid bacteria cells prepared in the above example were diluted within 2 to 10 times their weight and the cell concentration was adjusted to 1×10 ¹¹ cells/mL.

Serums for the control and experimental groups were prepared by a conventional method using the mixing amounts shown in Table 1 below.

[Table 1]

2-2. Whitening effect on hypermelanosis (in vivo)

The whitening effect of the cosmetic composition for skin whitening containing the heat-treated Lactobacillus rhamnosus LM1011 obtained in the above example as an active ingredient was confirmed through clinical experiments. The experiment was conducted on 23 people with hypermelanosis who met the selection criteria and were not included in the exemption criteria, and applied by self-application twice a day. The whitening effect was confirmed by M-value (hyperpigmentation improvement index) evaluation using Mexameter®, subject-specific evaluation, skin irritation evaluation by a dermatologist, and visual evaluation by a dermatologist.

Figure 3 shows the improvement rate of hyperpigmentation of the cosmetic composition for skin whitening by calculating the difference between the M-value before the test and the M-value after the test in the control area and the test area, respectively. This is a result showing the improvement rate over time by expressing it as a percentage compared to the measurement value before the test. Compared to the control placebo group, the improvement rate of hyperpigmentation after applying our cosmetic composition for skin whitening to the skin significantly increased with application time.

In Figure 4, the visual evaluation score of the cosmetic composition for skin whitening was obtained by calculating the values before and after the test in the control area and the test area, and then calculating the difference between these values. Compared to the control placebo group, the cosmetic composition for skin whitening of the present institution showed an increase in the degree of reduction in pigmentation depending on the application time.

The above results demonstrate that the cosmetic composition for skin whitening containing heat-treated Lactobacillus rhamnosus LM1011 as an active ingredient has a melanin reduction and consequent skin whitening effect when applied to the skin.

2-3. Brightening effect (in vivo)

The whitening effect of the cosmetic composition for skin whitening containing the heat-treated Lactobacillus rhamnosus LM1011 obtained in the above example as an active ingredient was confirmed through clinical experiments. The experiment was conducted on 23 people who met the selection criteria and were not included in the exemption criteria, and was applied by self-application method twice a day. The brightening effect was expressed as the skin brightness index improvement rate by measuring the brightness value (L-value) of the facial area using a chromameter.

In Figure 5, the brightening effect of the cosmetic composition for skin whitening was determined by calculating the difference between the L-value before and after the test in the control area and the test area, respectively. This is a result showing the improvement rate over time by expressing it as a percentage compared to the measurement value before the test. Compared to the control placebo group, the skin brightness index improvement rate of the skin whitening cosmetic composition of our clinic significantly increased after use. The above results demonstrate that there is a brightening effect when the cosmetic composition for skin whitening containing the heat-treated Lactobacillus rhamnosus LM1011 of the present application as an active ingredient is applied to the skin.

Above, embodiments of the present invention have been described with reference to the attached drawings, but those skilled in the art will understand that the present invention can be implemented in other specific forms without changing its technical idea or essential features. You will be able to understand it. Therefore, the embodiments described above should be understood in all respects as illustrative and not restrictive.

Claims (1)

A cosmetic composition for skin whitening containing Lactobacillus rhamnosus LM1011 as an active ingredient.