KR20230067352A - Selenium-enriched eggs with anticancer Properties and Production Methods of the eggs - Google Patents

Abstract

The present invention relates to a high-functional selenium egg with enhanced selenium content having anticancer activity using mineral water for livestock and a method for producing the same. It produces significantly higher Selen Eggs, which are about 1.43 to 1.75 times higher than normal eggs, and the mineral water for livestock according to the present invention has the effect of increasing egg production and improving egg quality.

Description

Selenium-enriched eggs with anticancer Properties and Production Methods of the eggs}

The present invention relates to a high-functional selenium egg with enhanced selenium content with anti-cancer activity and a production method, and more particularly, by supplying mineral water for livestock to laying hens continuously as negative water for at least 15 days or more so that the selenium content is reduced to that of ordinary eggs. It relates to selenium eggs that are significantly higher than 1.43 to 1.75 times or more and a method for continuously producing such eggs, and incidentally, by using mineral water for livestock for production of selenium eggs with enhanced selenium content according to the present invention, the egg production rate is increased. It relates to a method for increasing and improving the quality of eggs.

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등, 2020; Patra 등, 2020). Recently, as the strong efficacy of selenium (Se) as an anti-cancer nutrient has been reported, the development of anti-cancer drugs using it has begun, and with the importance of selenium, high-functional selenium eggs fortified with selenium have entered the global market (Zeng and Combs , 2008;

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et al., 2020; Patra et al., 2020).

ns Jakob Berzelius가 황산을 생산하던 중 처음 발견한 하였으며, 생체 내 셀레노 단백질(Selenoprotein)로 알려진 효소를 생성하기 위해 반드시 필요한 미량 영양소이다(도 1). Human cells need proper nutrition to maintain homeostasis. Micronutrients such as trace minerals, antioxidants, and vitamins are important for carrying out various cell regeneration processes, managing oxidative stress, and increasing immunity against pathogens. Selenium was discovered in 1818 by Swedish scientist J.

It was first discovered by ns Jakob Berzelius while producing sulfuric acid, and it is a micronutrient essential for producing enzymes known as selenoproteins in vivo (FIG. 1).

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등, 2020; Patra 등, 2020; Kieliszek 등, 2021). Selenoproteins are antioxidants that can inhibit the DNA-damaging ability of free radical compounds. Selenium is one of the trace minerals required to maintain very important functions in various physiological metabolic processes of the human body. A normal human body (weight 60-70 kg) contains 10-15 mg of selenium. Almost every cell in our body contains selenium, but most selenium is accumulated in the kidneys, liver, spleen, pancreas, and testicles. Selenium has about 1,800 times more antioxidant capacity than vitamin E. Selenium plays an important role in the pathogenesis and pathophysiology of various disorders due to its antioxidant properties, such as oxidative stress, inflammation, heart disease, rheumatoid arthritis, reproductive disorders, diabetes, thyroid, cancer and immune response. As humans age, their antioxidant capacity declines, and active oxygen damages white blood cells and lymphocytes, resulting in reduced immunity. Selenium has the effect of killing cancer cells. It produces a lot of antibodies through B lymphocytes, suppresses the activity of bacteria, and kills cancer cells by increasing lymphocytes and white blood cells. According to recent studies, selenium is known to help prevent heart disease and Alzheimer's disease. In 1,312 cancer patients, supplementation of 200 μg of Se in the daily diet showed up to 63%, 58%, and 46% reductions in tumor size in prostate, lung, and colon cancer, respectively (FIG. 2, FIG. 3, (Rayman, 2012 ;Csap'o and Albert, 2018; Guan et al., 2018; Kur

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et al., 2020; Patra et al., 2020; Kieliszek et al., 2021).

In 1980, the Food and Nutrition Committee of the US NRC announced the recommended daily intake of selenium. Recently, the recommended daily intake (RDA) of selenium is 55 μg, and the WHO and FAO suggest a maximum daily intake of 400 μg. Selenium intake of Koreans is insufficient. Since most of the foods we eat contain little selenium, it is necessary to develop functional foods to increase the intake of selenium. Today, innovative technological processes focused on the processing of foods rich in selenium in eggs, meat and dairy products have been successfully implemented in several countries. Eggs, beef, milk, pork and chicken fortified with selenium are now sold in 25 countries around the world. Considering the development of health functional foods, it has become clear that eggs rich in selenium content can be used to supplement micronutrient deficiencies and maintain the metabolic stability of the body (T´oth and Csap´o, 2018).

By feeding selenium feed additives, eggs, meat, or milk enriched with selenium content can be produced (Csap'o and Albert, 2018; Reference Patent Documents Korean Registered Patent No. 10-1160730, Korean Patent Publication No. 10-2005-0080380, Korea Registered Patent 10-0135958)

th and Csap'o, 2018). Selenium-fortified eggs with anti-cancer properties are available in markets around the world. One egg contains 3-25 micrograms of selenium, and according to the FDA, one egg can provide more than 20% of the RDA for selenium. The major types of selenium in eggs are selenocysteine, selenomethionine, and selenite, which consists of selenocysteine and selenomethionine, as the dominant species in egg yolk and egg white. Supplementation of selenium in the diet of laying hens can increase the selenium content of eggs to 0.34–0.58 ppm (Csap'o and Albert, 2018; T

th and Csap'o, 2018).

However, there is no known technology for producing highly functional selenium eggs with significantly increased selenium content with anti-cancer ability by supplying mineral water for livestock to laying hens and improving egg laying performance and egg quality.

1. Korea Patent No. 10-1160730 2. Korean Patent Publication No. 10-2005-0080380 3. Korea Patent No. 10-0135958

Accordingly, the present inventors provide highly functional selenium eggs with significantly enhanced selenium content by 1.43 to 1.75 times or more compared to normal eggs, which have anticancer activity, by continuously supplying them to laying hens for 15 days or more using mineral water for livestock. , or the present invention was completed by confirming that the egg production rate and egg quality could be improved when feeding the livestock mineral water to laying hens.

Therefore, the problem to be solved by the present invention is to provide a highly functional selenium egg with a significantly higher selenium content of 1.43 to 1.75 times higher than that of normal eggs and a method for producing the same, using mineral water for livestock ,

In addition, another technical problem of the present invention is to provide a method for increasing egg laying rate and improving egg quality using the mineral water of the present invention.

In order to achieve the above technical problem, the present invention is a high-functional selenium egg enriched with selenium, characterized in that the selenium content is 0.5 to 5.0 mg / L, preferably 2 to 4 mg / L, more preferably 3.34 mg / L provides mineral water for livestock, which is negative water for producing

In addition, the present invention supplies the above-described mineral water for livestock as negative water for laying hens for at least 15 days to produce highly functional selenium eggs having 1.43 to 1.75 times more selenium content than normal eggs with anticancer activity, and increase egg production It also provides ways to improve the general quality of eggs.

In addition, the present invention provides a highly functional selenium egg having selenium content of 1.43 to 1.75 times higher than that of a normal egg with anticancer activity produced by the production method as described above.

According to the present invention, it is possible to produce selenium eggs containing 1.43 to 1.75 times or more of selenium with anticancer activity compared to normal eggs, so that the biofunctional effect of selenium can be expressed only by ingesting such eggs.

In addition, the present invention can enhance the profitability of the poultry business because the production of high-functional selenium eggs with enhanced selenium content is possible while the egg laying rate is increased and the general quality of eggs is improved. Enhanced selenium eggs can contribute to the promotion of national health by being incorporated into various dishes, and can strengthen competitiveness even in various food industries where eggs are used, such as confectionery, baking, and noodle making.

Figure 1 shows the metabolic process of selenium in vivo.
Figure 2 shows the biological function of selenium.
Figure 3 shows the anti-cancer mechanism of selenium.
Figure 4 is a photograph confirming the internal quality of the selenium egg according to the present invention.

Hereinafter, specific details for carrying out the present invention will be described in detail.

The present invention is a method for producing high-functional selenium eggs with enhanced selenium with anti-cancer ability, producing silver, increasing the egg production rate and improving the general quality of eggs, in particular, the selenium content is significantly increased by 1.43 to 1.75 times or more compared to normal eggs In order to provide a production method, it is characterized in that the mineral water for livestock prepared according to the present invention is continuously supplied to laying hens as negative water for at least 15 days or more.

Mineral water for livestock according to the present invention for producing high-functional selenium-enhanced selenium eggs having the anti-cancer activity has a selenium content of 0.5 to 5.0 mg/L, preferably a selenium content of 2 to 4 mg/L. And, more preferably, the selenium content is 3.34 mg / L.

At this time, if the selenium content is less than the exemplified range, the selenium content of eggs is not sufficiently improved, and the increase in egg production and general quality of eggs may not reach the target, and if it exceeds the above range, in laying hens Selenium toxicity may occur, resulting in the loss of laying hens.

A specific method for producing the above-described mineral water according to the present invention is to extract silicate rock (collected from Chilbo mine, for example) and mineral-rich stone powder such as calcium, potassium, magnesium, and selenium using purified water, and filter it. And it can be prepared by dilution.

In more detail, the method for producing raw extraction water using mineral-rich stone powder is not particularly limited as long as it is widely known in the art to which the present invention belongs, but an efficient method is hot water extraction as a result of various tests by the present inventors,

Specifically, stone powder is put in purified water and heated at 100 ° C for 1 hour to extract selenium contained in raw water. At this time, if the temperature is low, the extraction efficiency may decrease, and if the heating temperature is too high, unnecessary energy waste occurs, and if the heating time is 1 hour, the elution of minerals is easy with the generation of bubbles, which is sufficient to obtain selenium. If the time is less than this, elution of minerals is difficult and selenium is lost, and if the heating time is longer than this, the time and production cost required for obtaining selenium may be exceeded.

Subsequently, the raw mineral water from which selenium is extracted to about 18 mg/L obtained by the heating process is filtered through gauze, and then filtered using a 4-stage filter to obtain a primary filtrate. It is composed of 78: 10: 10: 2 parts by weight in the order of charcoal powder: germanium: elvan: ocher.

The primary filtrate obtained by filtering in the above process is again passed through a 4-stage filter to obtain the secondary filtrate, and after obtaining the tertiary filtrate in the same way, mixing purified water with purified water and adjusting the dilution ratio, Mineral water is prepared for

The charcoal powder (oak or pine charcoal) is for preparing mineral water for livestock containing no off-flavor, clean, soft taste, and rich natural minerals and selenium. Hardwood charcoal powder is a natural material remaining after pyrolysis of cellulose at a high temperature of 1,200 ° C or higher, and contains abundant natural minerals with solid carbon 80-90 weight ratio and pH 8-9 alkalinity. In addition, since pores are formed between the particles of the hardwood charcoal powder, air containing oxygen is freely circulated, making it easy to obtain selenium.

The germanium is a material composed of carbon-based atoms together with carbon and silicon, and is very stable in a natural state. Anions contained therein are broken by an external force, and are used in the present invention to efficiently obtain selenium.

The elvan is a porous rock, and by capillary action, contaminants in water, heavy metals such as mercury are adsorbed and removed, and in particular, selenium is eluted as a mineral leaching action to increase mineral components in water.

The loess has a honeycomb structure with a wide surface and numerous pores are formed, and is spread on the surface of the filter canister and serves as a filter paper to efficiently purify selenium and emit far-infrared rays.

As described above, the dilution ratio is adjusted so that the selenium content of mineral water for livestock used as drinking water may be 0.5 to 5.0 mg/L, preferably 2 to 4 mg/L, and more preferably 3.34 mg/L.

If the livestock mineral water according to the present invention is used as negative water for laying hens, egg productivity of laying hens is increased, the quality of eggs produced by laying hens is improved, and the selenium content with anticancer activity is 1.43 compared to general eggs. It is possible to produce highly functional eggs enhanced by 1.75 to 1.75 times or more, which increases the rate of absorption and utilization of nutrients in the body, increases immunity, and vitality of microorganisms in the digestive tract, thereby increasing the absorption rate of selenium in the small intestine and the rate of transition to eggs during the metabolic process.

The mineral water for livestock according to the present invention is preferably supplied to laying hens as negative water without any particular restriction in intake for at least 15 days.

Eggs produced from laying hens raised by feeding mineral water for livestock according to the present invention as negative water were named 'SelenEggs' having anticancer ability. will be described in more detail by

These examples and experimental examples are only illustrative for understanding of the present invention, but do not limit the scope of the present invention, and the following examples may be configured so that various modifications can be made by selectively combining all or part of them.

< Example >

Manufacture of mineral water for livestock

Stone powder containing silicate rock rich in minerals such as calcium, potassium, magnesium, and selenium collected from the Chilbo mine was put into purified water and heated at 100 ° C for 1 hour to extract selenium contained in the raw water to prepare raw water. It was confirmed to contain about 18 mg/L of selenium.

Subsequently, 20 liters of the mineral water obtained by the heating process was filtered through gauze, and then, with a capacity of 1 kg, from the bottom layer, charcoal powder: germanium: elvan: ocher, using a four-stage filter consisting of a weight part of 78: 10: 10: 2 in order After filtering and repeating the first filtrate to obtain a second filtrate and a third filtrate, mineral water for livestock containing about 3.34 mg/L of selenium was prepared by mixing and diluting with purified water.

< Experimental Example >

Supply of livestock mineral water for laying hens

It was conducted at Kangwon National University as a joint research service of Whoinbio Co., Ltd. and Dume Farm.

Scientific procedures for laboratory animals were conducted according to the scientific and ethical procedures presented in NRC (2001). 360 45-week-old Hy-Line Brown hens were completely randomly assigned to 2 treatments (3 repetitions per treatment, 60 per repetition). The treatment group was divided into a control group (general drinking water feeding group) and a test group (mineral water feeding group for livestock) in the cage system in the same place.

For experimental feed, a commercial formulated feed (crude protein 17.00%, metabolic energy 2,750 kcal/kg) that meets the nutrient requirements of poultry presented by the US NRC (2001) was fed for 60 days. Water and experimental feed were freely consumed, and lighting was conducted for 16 hours a day (06:00-22:00).

After a week of adaptation to livestock mineral water, the test group was allowed to consume unlimited amounts of mineral water for livestock during the test period as drinking water.

After feeding mineral water for livestock for a certain period, that is, on the 15th, 30th, 45th, and 60th days of the test, the eggs laid were collected and the selenium content and egg quality were measured, respectively.

Selenium content in eggs

The selenium content of eggs was analyzed using an Inductively Coupled Plasma Optical Emission Spectroeter (ICP-OES), and the results are shown in Table 1 below.

The pretreatment process of eggs using ICP-OES was carried out by the microwave method according to the Food Code, and the conditions of the analysis equipment are as follows.

. Perkinelmer (Model : NexION 300D)

Stability: short term < 3% RSD, long term (4hr) < 4% RSD

Background Signal : <1 cps, at mass 220

· RF generator: 0.5-1.6KW, 40MHz

· Vacuum levels: 1×10-6 Torr or less

Referring to Table 1, according to the present invention, 30.17 to 36.95 μg of selenium with anticancer activity is contained per 60 g egg, and general negative water is 60 It can be seen that it contains 30.17 μg, which is 1.43 times higher than 21.07 μg of the control group fed daily.

In summary, by supplying the mineral water for livestock according to the present invention, it was possible to produce selenium eggs with a significantly increased selenium content of 1.43 to 1.75 times or more with anticancer activity compared to general eggs.

Egg General Quality Inspection

Egg quality measured by feeding the number of livestock minerals to laying hens for 60 days is shown in Table 2 and Figure 4.

Specifically, eggs produced from the 15th day after the supply of mineral water for livestock were collected, and the daily average egg laying rate and egg weight were calculated during the experiment period, and the quality of eggs (how many units, eggshell thickness, blueness, and egg weight) was calculated once every 15 days. Yellow index) was measured and expressed as an average value. Haugh unit (HU) indicates the freshness of an egg. By measuring the height of the thick egg white and the weight of the egg using a Haugh unit measuring device (FHK Co., Japan), [100log(H+7.57-1.7W0. 37) was calculated. where H is the height of the thick egg white (mm) and W is the weight of the egg (g). The egg yolk color was measured using Roche's egg color fan (Germany), and the egg yolk color was expressed as an average index (Egg yolk index). Egg shell thickness and blue intensity were measured using an egg quality measuring machine (FHK, Co., Japan). For egg shell thickness, the average value was calculated by measuring the blunt end, middle part, and tip of the egg shell 4 times each in the eggs used for HU and egg yolk measurement.

Looking at the results of Table 2, the egg quality was significantly increased in the test treatment group from the 30th day compared to the control group (P <0.05).

Summarizing the above-mentioned test results, the present invention can produce selenium eggs by containing 1.43 to 1.75 times or more of selenium with anti-cancer ability compared to normal eggs, so that the biofunctional effect of selenium can be obtained just by ingesting eggs according to the present invention. In addition, the present invention can enhance the profitability of the poultry business because it is possible to produce highly functional selenium eggs with enhanced selenium content while increasing the egg production rate and improving the general quality of eggs. Selenium eggs with enhanced selenium content can contribute to the promotion of national health by being incorporated into various dishes, and can enhance competitiveness even in various food industries such as confectionery, baking, and noodle making.

So far, the present invention has been looked at with respect to its preferred embodiments. Those skilled in the art to which the present invention pertains will be able to understand that the present invention can be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments should be considered from an illustrative rather than a limiting point of view. The scope of the present invention is shown in particular in the claims rather than the foregoing description, and all differences within the equivalent range will be construed as being included in the present invention.

Claims (8)

Mineral water for livestock, characterized in that the selenium content is 0.5 to 5.0 mg / L, which is negative for producing highly functional selenium eggs enriched with selenium with anticancer activity.
The mineral water for livestock according to claim 1, wherein the selenium content is 2 to 4 mg/L.
The mineral water for livestock according to claim 2, wherein the selenium content is 3.34 mg/L.
The method of claim 1, wherein the mineral water for livestock is prepared by putting stone powder containing mineral-rich silicate rock in purified water and heating at 100 ° C. for 1 hour to extract selenium to prepare mineral raw water, filter it with gauze, and then filter it with hardwood charcoal from the bottom layer. The first filtrate was obtained by filtering using a 4-stage filter tube composed of 78:10:10:2 by weight in the order of powder: germanium:elvan: loess, and then the final filtrate obtained by repeating the 4-stage filter tube twice was purified water. Mineral water for livestock, characterized in that prepared by diluting with.
A highly functional selenium egg produced using the mineral water for livestock according to any one of claims 1, 2 and 3 and enriched with selenium having anticancer activity.
The highly functional selenium egg according to claim 5, wherein the selenium content of the selenium having anticancer activity is 1.43 to 1.75 times that of a normal egg.
Drinking the livestock mineral water according to any one of claims 1, 2, and 3 to laying hens in order to increase the egg production rate, improve the general quality of eggs, and produce high-functional selenium eggs enriched with selenium having anti-cancer ability. Characterized by laying hens breeding method.
The method of claim 7, wherein the laying hens drink the mineral water for livestock for at least 15 days.

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