CN106259066A - A kind of cultural method of selenium-enriched sea cucumber - Google Patents

Abstract

The invention discloses a kind of selenium-rich method of Stichopus japonicus, cultivate including Stichopus japonicus selenium-rich and urge selenium to cultivate, it is characterized in that, selenium-rich cultivates or urges adds the yeast rich in selenium that organic selenium content accounts for more than the 80% of total Se content in the normal feedstuff thrown something and fed in selenium breeding process, the interpolation of yeast rich in selenium to selenium concentration is 0.80 mg/kg 1.0 mg/kg, the granular mixture that described yeast rich in selenium is made up with yeast rich in selenium of the gelatin thickening agent of gross mass 0.20 %.The present invention adds using yeast rich in selenium as external source selenium, and avirulence and sorption enhanced rate are low, safe to use；The interpolation concentration of yeast rich in selenium is tested through Concentraton gradient, and selenium concentration 0.80 mg/kg is possible not only to ensure apostichopus japonicus culture survival rate, improves Radix Morinae Bulleyanae enteral digestion enzymatic activity and vivo immunization enzymatic activity, and can be effectively improved the Se content in Radix Morinae Bulleyanae tissue；Selenium-enriched sea cucumber cultivation improves nutritive value and the culture benefit of Radix Morinae Bulleyanae, has good economic benefit and wide popularizing application prospect.

Description

A kind of cultural method of selenium-enriched sea cucumber

Technical field

The invention belongs to the cultural method of a kind of selenium-enriched sea cucumber, belong to mariculture technology field.

Background technology

Selenium is one of micronutrient element of wanting of needed by human body, and its physiological function can mainly be summarised as improving immunity of organism Power, prophylactic generation, dispelling toxicity, detoxicating.Along with the demand of health food is constantly increased by the most aging of China's population and people Adding, organic selenium and the natural functional food developmental research containing selenium product thereof become a focus of Nutritional studies, such as selenium-rich Folium Camelliae sinensis, Se-enriched egg, selenium-rich milk etc..

Marine organisms are one of human consumption's food main sources, and the developmental research to marine organisms food source is always nutrition Scholar's focus of attention.Selenium in many marine products is abundanter, but its content is also with the most difference of kind with the place of production. Radix Morinae Bulleyanae is as China's sea-farming Important Economic kind, and in addition to the nutritive value of current common cognition the most traditionally, internal selenium contains Amount has become one of excellent selenium supplement food.Set up the cultural method of a kind of stable healthy selenium-enriched sea cucumber, selenium-rich can be obtained Radix Morinae Bulleyanae, improves nutritive value and the culture benefit of Radix Morinae Bulleyanae, promotes the sustainable health development of Radix Morinae Bulleyanae industry.

Selenium-rich cultivation is main uses the selenium of inorganic selenium and two kinds of forms of organic selenium as the selenium additive in feedstuff.Inorganic selenium (sodium selenite etc.) toxicity is big, and using dosage difficulty is grasped and sorption enhanced rate is low, therefore, safe to use, easily for body suction Receipts, the organic selenium (the organic form selenium such as Selenonic protein, aminoacid) that biological activity is high become a kind of inevitable development trend.How Utilize external source selenium to add to increase Se content in Radix Morinae Bulleyanae body and secure good health selenium-enriched sea cucumber product, the most also without system maturation technology body System.

Summary of the invention

It is an object of the invention to provide the selenium-rich cultural method of a kind of Stichopus japonicus, it is adaptable to indoor and outdoor Radix Morinae Bulleyanae pond Omnidistance cultivation or short-term are supported temporarily thus are obtained selenium-enriched sea cucumber, improve nutritive value and the culture benefit of Radix Morinae Bulleyanae, promote Radix Morinae Bulleyanae industry Sustainable health development.

The selenium-rich method of this Stichopus japonicus, including Stichopus japonicus selenium-rich cultivation and urge selenium cultivate, it is characterised in that selenium-rich cultivate or Urge and the normal feedstuff thrown something and fed in selenium breeding process adds the yeast rich in selenium that organic selenium content accounts for more than the 80% of total Se content, rich The interpolation of selenium yeast to selenium concentration is 0.80 mg/kg-1.0 mg/kg, and described yeast rich in selenium is bright by gross mass 0.20 %'s The granular mixture that glue thickening agent and yeast rich in selenium are made.

Described selenium cultivation of urging is the concentration that the Stichopus japonicus before listing carries out surrounding by a definite date at indoor or outdoors holothurian pond Supporting temporarily, supporting environment water temperature temporarily is 16 ~ 22 DEG C, and dissolved oxygen is 6 ~ 8 mg/L, and pH is 7.8 ~ 8.2, and salinity is 31.0 ~ 32.0；Every day Absorb feces, and change water 1/3 ~ 1/2, within every two days, all change water once；It is added with the normal feedstuff twice of yeast rich in selenium every day (early 7 and late 16 points), feeding volume accounts for the 4.00% ~ 5.00% of Stichopus japonicus body weight the most respectively.

Though urge selenium cultivation not up to surrounding in Stichopus japonicus body Se content index terminate when meeting selenium-rich standard to urge selenium to cultivate；Urge In selenium culturing sea cucumbers body, Se content index reaches the one of following condition and is considered as meeting selenium-rich standard:

1) the digestive tract prolease activity of Stichopus japonicus and amylase activity increase to 2 times of the culturing sea cucumbers being not added with yeast rich in selenium with On；

2) in Stichopus japonicus body, SOD, GSH-Px, AKP, ACP immune enzyme activity of each tissue is significantly higher than and is not added with selenium yeast cultivation sea Ginseng；

3) total selenium and organic selenium content in Stichopus japonicus muscle > 20mg/kg(dry weight):

4) total selenium and organic selenium content in wall of sea cucumber Stichopus japonicus > 5mg/kg(dry weight).

Present invention is characterized in that 1, add to apostichopus japonicus Selenka feed as organic selenium source using yeast rich in selenium, compare inorganic selenium without Toxicity and sorption enhanced rate are low, safe to use, are preferable selenium supplements.2, fed by continuous 4 weeks and urge selenium, can obtain Total selenium and organic selenium content in muscle > 20mg/kg, total selenium and organic selenium content in body wall > the selenium-rich Radix Morinae Bulleyanae of more than 6mg/kg. 3, by this cultural method, be possible not only to improve Se content in Radix Morinae Bulleyanae body, and improve Radix Morinae Bulleyanae enteral digestion enzymatic activity and Vivo immunization enzymatic activity, ensures the cultivation of Radix Morinae Bulleyanae efficient and healthful.

Detailed description of the invention

A kind of selenium-rich method of Stichopus japonicus, embodiment is as follows:

1) yeast rich in selenium is purchased from Angel Yeast Co., Ltd, and total selenium actual content is 1408.80 ± 0.47mg/ after measured Kg, wherein organic selenium content is 1141.13 ± 0.26mg/kg, and inorganic selenium content is 267.67 ± 0.58mg/kg；

2) experiment often 20 Radix Morinae Bulleyanaes of group, are divided into 7 groups, and often group 3 is parallel, takes the method amplified step by step to add in normal feedstuff Adding yeast rich in selenium, each group dietary Se adds concentration and is followed successively by 0(blank), 0.20,0.40,0.80,1.60,3.20, 6.40mg/kg；

3) apostichopus japonicus culture environment water temperature is 17 ~ 19 DEG C, and dissolved oxygen is 6 ~ 8 mg/L, and pH is 7.8 ~ 8.2, salinity is 31.0 ~ 32.0.Absorb feces every day, and change water about 1/3 ~ 1/2, within every two days, all change water once.Bait throwing in every day twice (early 7 and evening 16 points), the 4.00% ~ 5.00% of children's ginseng body weight of throwing something and feeding the most respectively, depending on concrete feeding volume is according to the situation of ingesting, carry out 4 weeks Administering transgenic.Stopping throws something and feeds adds feedstuff sampling and measuring after 1 week of yeast rich in selenium；

4) each experimental group survival rate of Apostichopus japonicus is more than 85%, and selenium level 0.80 mg/kg, 1.60 mg/kg feedstuff group Radix Morinae Bulleyanaes are without extremely Dying phenomenon, survival rate is up to 100%, and this group Radix Morinae Bulleyanae histone enzyme, amylase activity respectively reach the highest, are 35.13 U/mg Prot, 0.51 U/mg prot, respectively the 2.45 of matched group, 2.07 times, SOD, GSH-Px, AKP, ACP in each tissue are also Reach the highest, be significantly higher than other groups；After selenium-rich cultivates in selenium level 0.80 mg/kg group, Apostichopus japonicus muscle and body wall In total selenium and organic selenium content the highest, be all remarkably higher than other group (P ＜ 0.05)；Wherein the total selenium of muscle and organic selenium increase To 26.44,25.17 mg/kg, in body wall total selenium and organic selenium increase to 5.78,5.07mg/kg.

In the present embodiment, it is that organic selenium source carries out Radix Morinae Bulleyanae selenium-rich cultivation by adding yeast rich in selenium at feedstuff, is improving While Radix Morinae Bulleyanae immunocompetence ensures efficient and healthful cultivation, main edible Se content in tissue muscle and body wall in Radix Morinae Bulleyanae body It is greatly improved, improves nutritive value and the culture benefit of Radix Morinae Bulleyanae, there is good economic benefit and wide popularization Application prospect.

Claims (3)

1. a selenium-rich method for Stichopus japonicus, cultivates including Stichopus japonicus selenium-rich and urges selenium to cultivate, it is characterised in that selenium-rich cultivates or urges The normal feedstuff thrown something and fed in selenium breeding process adds the yeast rich in selenium that organic selenium content accounts for more than the 80% of total Se content, selenium-rich The interpolation of yeast to selenium concentration is 0.80 mg/kg-1.0 mg/kg, and described yeast rich in selenium is by the gelatin of gross mass 0.20 % The granular mixture that thickening agent and yeast rich in selenium are made.

2. according to the selenium-rich method of a kind of Stichopus japonicus described in claim 1, it is characterised in that described urge selenium cultivation be by The concentration that Stichopus japonicus before listing carries out surrounding by a definite date at indoor or outdoors holothurian pond is supported temporarily, and supporting environment water temperature temporarily is 16 ~ 22 DEG C, Dissolved oxygen is 6 ~ 8 mg/L, and pH is 7.8 ~ 8.2, and salinity is 31.0 ~ 32.0；Absorb feces every day, and change water 1/3 ~ 1/2, every two It all changes water once；It is added with the normal feedstuff twice (early and late 16 points) of yeast rich in selenium, feeding volume the most respectively every day at 7 Account for the 4.00% ~ 5.00% of Stichopus japonicus body weight.

The selenium-rich method of a kind of Stichopus japonicus the most according to claim 2, it is characterised in that though urging selenium cultivation not up to surrounding But Se content index terminates when meeting selenium-rich standard to urge selenium to cultivate in Stichopus japonicus body；In urging selenium culturing sea cucumbers body, Se content index reaches The one of following condition is considered as meeting selenium-rich standard:

1) the digestive tract prolease activity of Stichopus japonicus and amylase activity increase to 2 times of the culturing sea cucumbers being not added with yeast rich in selenium with On；

2) in Stichopus japonicus body, SOD, GSH-Px, AKP, ACP immune enzyme activity of each tissue is significantly higher than and is not added with selenium yeast cultivation sea Ginseng；

3) total selenium and organic selenium content in Stichopus japonicus muscle > 20mg/kg:

4) total selenium and organic selenium content in wall of sea cucumber Stichopus japonicus > 5mg/kg.