CN109497351A - A kind of functional fish meal and preparation method thereof based on selenium-rich chlorella - Google Patents
A kind of functional fish meal and preparation method thereof based on selenium-rich chlorella Download PDFInfo
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Abstract
A kind of functional fish meal and preparation method thereof based on selenium-rich chlorella, contain rapeseed dregs, rice bran, flour, fish meal, Soybean Meal, composite mineral matter, multi-vitamins, calcium dihydrogen phosphate, choline chloride and selenium-rich chlorella powder in formula, the selenium-rich chlorella powder, by adding the culture of 2mg/L sodium selenite in BG-11 culture medium with optimal conditions, high-pressure homogeneous broken wall is obtained after finally digesting.Utilize the high accumulation selenium ability and conversion organic selenium ability of chlorella, effectively improve the Se content and organic selenium ratio in feed, enhance safety and the bioavailability of selenium, while promoting fish constitution and meat, further increase economic benefit, and the increase of culture medium Se content can also promote the synthesis of chlorella astaxanthin, increase the immune function of fish body.Fish rich in organic selenium are enhancing human immunity, anti-aging, anticancer aspect, provide technical support for selenium-rich fish food industrialized developing.
Description
Technical field
The present invention relates to aquatic feeds field more particularly to a kind of functional fish meals and its system based on selenium-rich chlorella
Preparation Method.
Background technique
Selenium is a kind of required microelement in human body and animal body, has very strong oxidation resistance, it can form life
Antioxidase in object can protect cell membrane from oxidative damage, keep its permeability；In addition selenium-P albumen can chelate heavy metal
Equal poisonous substances reduce poisonous substance toxicity effect.Therefore, selenium is referred to as " king of anti-cancer " in body trace element by scientist.Selenium
It can be used in aquaculture, the intracorporal free radical of animal can be removed, keep animal more vigourous.But according to statistics, the whole world has
A countries and regions selenium deficiency more than 40, China are the areas of selenium deficiency most serious in the world, and the province of selenium deficiency accounts for about national total area
72%, wherein 30% is serious selenium deficiency area.Some areas of 22 provinces and cities, China, about 700,000,000 people live in low selenium band.
Selenium is totally divided into organic selenium and inorganic selenium two major classes, and inorganic selenium is mainly deposited in the form of selenite and selenate
, but utilization rate is lower, improper use is also easy to produce toxicity, therefore can not directly be used by the mankind；And organic selenium be present in it is dynamic
In plant, there is higher safety and bioavailability, be more suitable for the selenium-supply requirement of human body and animal body.
Astaxanthin has stronger antioxidant activity, this allows for astaxanthin in coloring, anti-oxidant, light protection, wrinkle resistant, promotion
Growth, enhancing is immune, antitumor, cardiovascular and cerebrovascular and maintenance central nervous system health etc. is protected to have excellent biology
Function can also be used as the application such as free radical scavenger and immunomodulator, it is clear that it is also pole for the health for ensureing human body
It is beneficial.Therefore, depth and is had very wide by the concern of the industries such as domestic and international food, medicine, cosmetics, aquaculture
Wealthy application prospect.
Chlorella is a kind of important microalgae resource, protein rich in, amino acid, pigment, polyunsaturated fat
Acid etc. has nutritional ingredient abundant, while also can produce astaxanthin, is a kind of ideal feedstuff of aquaculture field.
In animal feed add chlorella can significantly improve the immunity, survival rate and yield of animal, and can promote feed intake,
It improves the speed of growth and reduces disease.
Certain density sodium selenite is added in bead algae culture medium, will not influence the normal production of chlorella, even
Chlorella can also be promoted to generate astaxanthin, and convert organic selenium deposition in vivo for inorganic selenium.Sodium selenite is in chlorella
It can be metabolized in vivo and generate selenomethionine and selenocysteine, may participate in organism and be further metabolized utilization.
Summary of the invention
It is an object of the invention to: a kind of functional fish meal and preparation method thereof based on selenium-rich chlorella is provided,
While enhancing fish culture advantage, a kind of safe selenium supplement food is provided using fish containing selenium as carrier, for selenium deficiency crowd
Product source.The Tilapia mossambica of feed cultivation of the present invention is rich in organic selenium, and bioavilability is high, edible safety, and easily promoting cultivation makes
With.
To achieve the above object, proposed by the present invention this based on the functional fish meal of selenium-rich chlorella and its preparation side
Method, it is characterised in that: the fish meal includes: rapeseed dregs, rice bran, flour, fish meal, Soybean Meal, composite mineral matter, di(2-ethylhexyl)phosphate
Hydrogen calcium, choline chloride, multi-vitamins and selenium-rich chlorella powder；The mass percent of the formula of its fish meal are as follows:
Rapeseed dregs: 20-30%；
Rice bran: 20-30%；
Flour: 15-20%；
Fish meal: 8-20%；
Soybean Meal: 10-15%；
Composite mineral matter: 0.1-0.3%；
Calcium dihydrogen phosphate: 1-2%；
Choline chloride: 0.2-1%；
Multi-vitamins: 0.1-0.15%；
Selenium-rich chlorella powder: 2-3%.
The multi-vitamins include vitamin A, vitamin C, vitamin D, vitamin E, vitamin B1, vitamin
B2, vitamin B3, vitamin B6, vitamin B7, vitamin B11, vitamin B12.
The preparation method comprises the following steps:
1) selenium-rich chlorella powder is prepared
Under conditions of light passing ventilation, pH6.5~7.5,27~30 DEG C of cultivation temperature, it is added in BG-11 culture medium
After 2mg/L sodium selenite culture 90~120 hours, under the conditions of optimizing broken wall, addition additive amount is chlorella stock solution quality 0.5
~1.0% composite plant hydrolase and additive amount is the cellulase degradation broken wall of chlorella stock solution quality 0.5~1.0%,
The chlorella powder collected after high-pressure homogeneous broken wall is carried out by GYB30-6A high pressure homogenizer again.
2) fish meal is prepared
According to mass percent meter, smashed feedstuff: rapeseed dregs 20-30.5%, rice bran 20-30%, flour is taken
15-20%, fish meal 8-20%, Soybean Meal 10-15%, composite mineral matter 0.1-0.3%, calcium dihydrogen phosphate 1-2%, choline chloride
0.3%, multi-vitamins 0.1-0.15%, selenium-rich chlorella powder 1-3%, the above ingredient is mixed, suitable quantity of water is added to stir evenly,
It is granulated to obtain the suitable fish meal of granular size with pelletizer again.
Preferably, above-mentioned optimization broken wall condition is as follows: composite plant hydrolysis activity is the U of 0.3 ten thousand U~1.0 ten thousand, cellulose
Enzyme activity is that 0.3 ten thousand U~1.0 ten thousand U, pH4~6,30~60 DEG C of heated at constant temperature digest 3~6 hours；PH4.5,15MPa, 25 DEG C,
High-pressure homogeneous circulation is three times.
The advantages and positive effects of the present invention are:
1, the present invention passes through the sodium selenite for adding 2mg/L in the medium, richness in chlorella powder finally obtained in this way
Selenium concentration is 65ug/g or so, is 590 times of selenium concentration in the chlorella of non-selenium-rich culturing；Astaxanthin concentration reached at 14 days
4.99mg/g or so is 5.9 times of astaxanthin concentration in normal chlorella.
2, chlorella Se-enriched feedstuff provided by the invention can significantly enhance the intracorporal Se content of fish and astaxanthin
Content enhances fish body matter and meat.Selenium energy antagonism heavy metal Hg and arsenic mitigate toxic action of the heavy metal to aquatic livestock, shrimp
Green element can enhance oxidation resistance.Fish can be made, and immunity, growth rate and survival rate are all greatly improved during the feeding process, increase
Economic benefit.
3, in the selenium-rich chlorella powder that the present invention produces, chlorella cells can reach the conversion ratio of inorganic selenium
60.14% or so, three kinds of organic Se forms are primarily formed, remaining selenium element exists in the form of acid/mineral compounds.With
During feed raising Tilapia mossambica of the present invention, the inorganic matter chemical combination selenium element of low concentration will not influence the normal cultivation of fish body, and
Inorganic selenium can also be absorbed in fish body and is converted into organic selenium, further promote the organic selenium content in the flesh of fish, it is edible to can be used for human body.
The bioavilability of organic selenium is high, edible safety, and easily promotes cultivation, is suitble to the selenium-supply approach of human body.
Specific embodiment
Below with reference to example, the invention will be further described.
This functional fish meal and preparation method thereof based on selenium-rich chlorella proposed by the present invention, core intention exists
In: utilize common fish meal raw material rapeseed dregs, rice bran, flour, fish meal, Soybean Meal, composite mineral matter, calcium dihydrogen phosphate, chlorine
Changing choline, multi-vitamins etc. is raw material, utilizes the richness obtained through sodium selenite culture, enzymatic hydrolysis, high-pressure homogeneous broken wall treatment
The astaxanthin being rich in selenium chlorella powder and chlorella powder is fabricated to as intermediate vector rich in organic selenium and astaxanthin
Fish feed, and by obtaining people by fish product acquisition of cooking to human health needs with the fish of above-mentioned forage feed
Organic selenium and astaxanthin.
This functional fish meal and preparation method thereof based on selenium-rich chlorella, it is characterised in that: the fish meal
Include: rapeseed dregs, rice bran, flour, fish meal, Soybean Meal, composite mineral matter, calcium dihydrogen phosphate, choline chloride, multi-vitamins and
Selenium-rich chlorella powder；The mass percent of the formula of its fish meal are as follows:
Rapeseed dregs: 20-30%；
Rice bran: 20-30%；
Flour: 15-20%；
Fish meal: 8-20%；
Soybean Meal: 10-15%；
Composite mineral matter: 0.1-0.3%；
Calcium dihydrogen phosphate: 1-2%；
Choline chloride: 0.2-1%；
Multi-vitamins: 0.1-0.15%；
Selenium-rich chlorella powder: 2-3%.
The multi-vitamins include vitamin A, vitamin C, vitamin D, vitamin E, vitamin B1, vitamin
B2, vitamin B3, vitamin B6, vitamin B7, vitamin B11, vitamin B12.
Specifically the production method is as follows:
1) selenium-rich chlorella powder is prepared
Under conditions of light passing ventilation, pH6.5~7.5,27~30 DEG C of cultivation temperature, it is added in BG-11 culture medium
After 2mg/L sodium selenite culture 90~120 hours, under the conditions of optimizing broken wall, addition additive amount is chlorella stock solution quality 0.5
~1.0% composite plant hydrolase and additive amount is the cellulase degradation broken wall of chlorella stock solution quality 0.5~1.0%,
The chlorella powder collected after high-pressure homogeneous broken wall is carried out by GYB30-6A high pressure homogenizer again.
2) fish meal is prepared
According to mass percent meter, take following smashed feedstuffs: rapeseed dregs 20-30.5%, rice bran 20-30%,
Flour 15-20%, fish meal 8-20%, Soybean Meal 10-15%, composite mineral matter 0.1-0.3%, calcium dihydrogen phosphate 1-2%, chlorination
Choline 0.3%, multi-vitamins 0.1-0.15%, selenium-rich chlorella powder 1-3%, the above ingredient is mixed, suitable quantity of water is added to stir
Uniformly, it then is granulated to obtain the suitable fish meal of granular size with pelletizer.
Embodiment 1:(is for feeding Tilapia mossambica)
A kind of functional fish meal based on selenium-rich chlorella takes following smashed feeds former by mass percentage
Material: rapeseed dregs 29.5%, rice bran 28%, flour 15%, fish meal 10%, Soybean Meal 14%, composite mineral matter 0.1%, di(2-ethylhexyl)phosphate
Hydrogen calcium 1%, choline chloride 0.3%, multi-vitamins 0.1%, selenium-rich chlorella powder 2%.The above ingredient mixing, is added suitable quantity of water
It stirs evenly, then is granulated with pelletizer.
By taking the Rofe fry of 4-5cm or so as an example, feed of the present invention and normal diet two months are fed, are checked in fish body
The data such as flesh of fish Se content and fish body growth indexes, it is as shown in table 1 to count other indexs:
Functional fish meal culture efficiency of the table 1 based on selenium-rich chlorella
The cultivation example shows feeding feed of the present invention, can effectively improve Se content in Tilapia mossambica body, flesh of fish Se content
It is that normal diet feeds 2.67 times of fish, rate of body weight gain is also 1.23 times that normal diet feeds fish, and the survival rate of Tilapia mossambica, fish
Skin content astaxanthin is above normal diet and feeds fish, has reached the purpose of the present invention.
Embodiment 2:(is for feeding Tilapia mossambica)
Further, taking following smashed feedstuffs: rapeseed dregs 21%, rice bran 21%, face according to mass percent meter
It is powder 19%, fish meal 19%, Soybean Meal 14%, composite mineral matter 0.2%, calcium dihydrogen phosphate 2%, choline chloride 0.65%, compound
Vitamin 0.15%, selenium-rich chlorella powder 3%.The above ingredient mixing, is added suitable quantity of water and stirs evenly, then be granulated with pelletizer.
By taking the Rofe fry of 4-5cm or so as an example, feed of the present invention and normal diet two months are fed, are checked in fish body
The data such as flesh of fish Se content and fish body growth indexes, it is as shown in table 2 to count other indexs:
Functional fish meal culture efficiency of the table 2 based on selenium-rich chlorella
The cultivation example shows feeding feed of the present invention, can effectively improve Se content in Tilapia mossambica body, flesh of fish Se content
It is that normal diet feeds 3.4 times of fish, rate of body weight gain is also 1.4 times that normal diet feeds fish, and the survival rate of Tilapia mossambica, fish-skin
Content astaxanthin is above normal diet and feeds fish, has reached the purpose of the present invention.
From the feed that can be seen that the method for the present invention preparation in the data comparison of the embodiment of the present invention 1,2 and normal diet
It is more beneficial to Tilapia mossambica, there is remarkable result on meat Se content, content astaxanthin and rate of body weight gain, therefore, it can be seen that this
Inventive method is conducive to improve prepared feed to the growth performance of Tilapia mossambica by adding selenium-rich chlorella powder in the feed
And quality.
Examples detailed above has carried out exemplary description to the present invention, only technical concepts and features to illustrate the invention, but this
The specific implementation of invention is not subject to the restrictions described above, as long as using the scheme of the inventive concept and technical scheme of the present invention
The various improvement carried out, or it is not improved directly applying to other occasions, it is within the scope of the present invention.
Claims (4)
1. a kind of functional fish meal and preparation method thereof based on selenium-rich chlorella, it is characterised in that: the fish meal packet
It includes: rapeseed dregs, rice bran, flour, fish meal, Soybean Meal, composite mineral matter, calcium dihydrogen phosphate, choline chloride, multi-vitamins and richness
Selenium chlorella powder；The mass percent of the formula of its fish meal are as follows:
2. a kind of functional fish meal and preparation method thereof based on selenium-rich chlorella according to claim 1, feature
Be: the multi-vitamins include vitamin A, vitamin C, vitamin D, vitamin E, vitamin B1, vitamin B2, dimension
Raw element B3, vitamin B6, vitamin B7, vitamin B11, vitamin B12.
3. a kind of functional fish meal and preparation method thereof based on selenium-rich chlorella, which is characterized in that the preparation method is such as
Under:
1) selenium-rich chlorella powder is prepared
Under conditions of light passing ventilation, pH6.5~7.5,27~30 DEG C of cultivation temperature, it is sub- that 2mg/L is added in BG-11 culture medium
After sodium selenate culture 90~120 hours, under the conditions of optimizing broken wall, addition additive amount is chlorella stock solution quality 0.5~1.0%
Composite plant hydrolase and additive amount be chlorella stock solution quality 0.5~1.0% cellulase degradation broken wall, then pass through
GYB30-6A high pressure homogenizer carries out the chlorella powder collected after high-pressure homogeneous broken wall；
2) fish meal is prepared
According to mass percent meter, smashed feedstuff: rapeseed dregs 20-30.5%, rice bran 20-30%, flour 15- is taken
20%, fish meal 8-20%, Soybean Meal 10-15%, composite mineral matter 0.1-0.3%, calcium dihydrogen phosphate 1-2%, choline chloride
0.3%, multi-vitamins 0.1-0.15%, selenium-rich chlorella powder 1-3%, the above ingredient is mixed, suitable quantity of water is added to stir evenly,
It is granulated to obtain the suitable fish meal of granular size with pelletizer again.
4. a kind of functional fish meal and preparation method thereof based on selenium-rich chlorella according to claim 3, feature
Be: the chlorella optimizes broken wall condition are as follows: composite plant hydrolysis activity is the U of 0.3 ten thousand U~1.0 ten thousand, cellulase activity
It is digested 3~6 hours for U, pH4~6,30~60 DEG C of heated at constant temperature of 0.3 ten thousand U~1.0 ten thousand；PH4.5,15MPa, 25 DEG C, high pressure are equal
Matter recycles three times.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110317769A (en) * | 2019-07-18 | 2019-10-11 | 恩施硒圣植物科技有限公司 | A kind of cultural method of selenium-rich microalgae |
| CN114009628A (en) * | 2021-11-05 | 2022-02-08 | 中国水产科学研究院黑龙江水产研究所 | Carp feed for improving muscle quality and preparation method thereof |
| CN114196544A (en) * | 2021-08-04 | 2022-03-18 | 北部湾大学 | Chlorella and chlorella powder and preparation method thereof |
| CN118248245A (en) * | 2024-05-21 | 2024-06-25 | 中国水产科学研究院南海水产研究所 | Chlorella-based prawn feed preparation optimization method and system |
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| CN107384907A (en) * | 2017-07-13 | 2017-11-24 | 荆楚理工学院 | A kind of preparation method of selenium-rich astaxanthin chlorella powder |
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| CN114009628A (en) * | 2021-11-05 | 2022-02-08 | 中国水产科学研究院黑龙江水产研究所 | Carp feed for improving muscle quality and preparation method thereof |
| CN118248245A (en) * | 2024-05-21 | 2024-06-25 | 中国水产科学研究院南海水产研究所 | Chlorella-based prawn feed preparation optimization method and system |
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