KR20230022515A - Cosmetic composition comprising dogo hot spring water - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising, as active ingredients: Dogo hot spring water; and a fermented extract of at least one medicinal herb selected from Paeonia suffruticosa root bark, Acer palmatum Thunb., and Forsythia suspense fruit. The cosmetic composition of the present invention inhibits the production of MMP-1 protein, promotes collagen production, and has an ability to inhibit tyrosinase and melanin production, and thus has excellent skin whitening and wrinkle reduction effects.

Description

Cosmetic composition containing Dogo hot spring water {COSMETIC COMPOSITION COMPRISING DOGO HOT SPRING WATER}

The present invention relates to a cosmetic composition containing Dogo hot spring water, and more particularly, to a cosmetic composition containing Dogo hot spring water and a fermented herbal medicine.

The skin is the largest organ in the body and always directly in contact with the external environment, playing the role of a protective film to protect the living body from various stimuli or dry environments. In addition, it protects the body from physical abrasions, prevents loss of moisture inside the body, protects from ultraviolet rays generated by the sun, and plays a very important role in regulating the body temperature.

Such skin causes problems such as skin contamination, dryness, trouble, skin immune system abnormality, etc. due to various physical factors, external environmental factors, infections, and the like. In particular, with the rapid industrial development of modern times, pollution problems such as air pollution and water pollution have become serious, and the strong cooling and heating of the living space due to the improvement of the residential environment causes the skin to become rough, dry, and aging. are greatly influenced by Human skin gradually becomes thinner due to intrinsic aging and photoaging, wrinkles increase, elasticity decreases, and spots and freckles increase due to exposure to ultraviolet rays. In particular, endogenous aging occurs when the secretion of hormones decreases or the function and activity of immune cells decrease, resulting in a decrease in the biosynthesis of biocomponent proteins, which progresses rapidly with an increase in active oxygen.

It is known that humans constantly generate reactive oxygen species in the metabolic process, which act as direct or indirect causes of aging. In order to prevent skin aging, various antioxidants are used in cosmetics, and typical synthetic antioxidants include butylated hydroxytoluene (BHT), butylated hydroxyanisole (BHA), and tertiary butylhydroquinone (TBHQ). In general, synthetic antioxidants are widely used due to their excellent effects and low prices, but their use is limited because they are known to cause liver enlargement, fatty mutations, and cancer as well as self-toxicity when in oral or skin contact.

Niacinamide and arbutin, which are used as raw materials for whitening functional cosmetics, and retinol, which are used as raw materials for wrinkle-improving functional cosmetics, have a problem in that the whitening effect is reduced due to the destruction of ingredients by oxidation and the restriction of the mixing amount due to skin irritation. Recently, as various cosmetic compositions using natural materials have appeared on the market, consumers have become interested in products using natural materials rather than products made of existing chemical materials, and accordingly, cosmetic compositions using various natural materials are being manufactured.

In addition, the anti-wrinkle effect of cosmetics is based on the effect of improving the dermis layer by various active ingredients in addition to the temporary effect of moisturizing by moisturizing factors, and the growth of retinol, vitamin C, and EGF, which are representative raw materials of wrinkle-improving cosmetics, Factor components are well known to exhibit anti-wrinkle effects by increasing collagen generation from human dermal fibroblasts. In order to prevent this phenomenon and maintain a healthier and more beautiful skin, physiologically active substances obtained from various known animals, plants, microorganisms, etc. are added to cosmetics and used to maintain the unique function of the skin and activate skin cells to Efforts have been made to effectively inhibit aging.

In the field of cosmetics, research and development on functional cosmetics having whitening and wrinkle-improving effects are being actively conducted, and in particular, studies using natural substances are continuing to avoid toxicity or irritation to the skin.

Korean Patent Registration No. 10-2059626 Korean Patent Registration No. 10-1774348

An object of the present invention is to provide a cosmetic composition containing Dogo hot spring water and herbal medicine extract as active ingredients, which has a skin whitening or wrinkle improvement effect.

According to one aspect of the invention,

Dogo hot spring water; And a cosmetic composition containing as an active ingredient is provided; and at least one herbal medicine fermented extract selected from among mudanpi, gyejochuk and yeongyo.

The fermented extract of the herbal medicine may be an extract of fermented black yeast ( Aureobasidium pullulans ).

The extract may be extracted with water, alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof.

The herbal medicine fermented extract may be a mixture of a fermented extract of mudanpi, a fermented extract of gyejochuk, and a fermented extract of Yeongyo.

The fermented extract of the herbal medicine may include 50 to 150 parts by weight of the fermented extract of Gyejochu, and 50 to 150 parts by weight of the fermented extract of Yeongyo, based on 100 parts by weight of the fermented extract of Mudanpi.

The Dogo hot spring water and the fermented herbal medicine extract may be mixed in a weight ratio of 6:4 to 8:2.

The Dogo hot spring water contains 55 to 65 mg/L of calcium (Ca), 25 to 35 mg/L of sodium (Na), 10 to 20 mg/L of silicon (Si), 0.001 to 0.05 mg/L of lithium (Li) and strontium ( Sr) 1.0 to 2.0 mg/L.

The cosmetic composition may be used for skin whitening or wrinkle improvement.

The cosmetic composition containing Dogo hot spring water of the present invention inhibits the production of MMP-1 protein, promotes collagen production, and has an ability to inhibit tyrosinase and melanin production, so it has excellent skin whitening and wrinkle improvement effects.

Since the present invention can apply various transformations and have various embodiments, specific embodiments will be illustrated in the drawings and described in detail in the detailed description. However, it should be understood that this is not intended to limit the present invention to specific embodiments, and includes all transformations, equivalents, and substitutes included in the spirit and scope of the present invention. In describing the present invention, if it is determined that a detailed description of related known technologies may obscure the gist of the present invention, the detailed description will be omitted.

Hereinafter, a cosmetic composition containing the Dogo hot spring water of the present invention will be described.

The cosmetic composition of the present invention is Dogo hot spring water; and at least one fermented herbal medicine extract selected from among Moutanpi, Gyejochuk, and Yeongyo.

The fermented extract of the herbal medicine may be an extract of fermented black yeast ( Aureobasidium pullulans ).

The extract may be extracted with water, alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof, and preferably may be a hot water extract.

The fermented extract of medicinal herbs is preferably a mixture of fermented extract of mudanpi, fermented extract of gyejochuk, and fermented extract of Yeongyo.

The fermented extract of the herbal medicine may include 50 to 150 parts by weight of fermented extract of Gyejochu, and 50 to 150 parts by weight of fermented extract of Yeongyo, based on 100 parts by weight of fermented extract of Mudanpi, preferably based on 100 parts by weight of fermented extract of Mudanpi. , 70 to 130 parts by weight of the gray scale, and 70 to 130 parts by weight of the fermented extract of the annual bridge, more preferably 90 to 110 parts by weight of the fermented extract of the gray scale axis, and 90 to 110 parts by weight of the fermented extract of the annual bridge, relative to 100 parts by weight of the fermented extract of moutanpi can be wealth

If any one of the three types of fermented herbal medicinal extracts is excluded or out of the above content, the wrinkle improvement or whitening effect may be greatly reduced compared to the three types of fermented herbal medicinal extracts within the above content range.

Moutan bark is a medicinal material made from the root bark of the peony (Paeonia suffruticosa Andrews) of the peony family, and is used in China and Japan as well as in Korea. Mokdanpi is used for menstrual irregularities due to blood fever, menstrual cramps, bruises, hematemesis, nosebleeds, spots, bone aches due to fever, blood pressure increase, blood elimination, bruises, anti-inflammatory analgesia, abscess treatment, and early appendicitis. It is known to relieve chest discomfort. As for the pharmacological action, analgesic, sedative, antipyretic, anticonvulsant, anti-inflammatory, antithrombotic, anti-allergic, gastric juice secretion inhibition, uterine mucosal congestion, and antibacterial action have been reported. Appearance is a piece of tubular bark, the outer surface is dark brown or purplish brown, and there are horizontally long and small oval side root marks and vertical wrinkles. The inner surface is light grayish brown or dark purple, flat, and the bent surface is rough. White crystals are sometimes attached to the inside and bent sides.

The gradation axis is Manshurian fullmoon maple of the Maple family, and its scientific name is Acer pseudo-sieboldianum (Paxton) Komarov. Deciduous broad-leaved small tree, height of about 8m, leaves are 7-10cm long, divided into 9-10 branches, hairs grow densely on the backside of young leaves and branches, flowers are hermaphrodite, magenta in May, calyx is There are 5-6 hairs, and the petals are composed of 4 sheets. The bark part of the herbal medicinal material is used, and the bark part is also used for the extract of the goldenrod of the present invention.

Yeongyo refers to the dried fruit of Forsythia viridissima Lindley or Forsythia suspensa Vahl of the ash family. Yeongyo got its name because the fruit forms a room similar to a lotus flower and protrudes long among various grasses. It has a peculiar smell, the taste is bitter, and the energy is slightly cold. It is known that it has the effect of lowering heat and detoxifying, eliminating wind fever, removing boils or wounds, or releasing clumps or lumps. As for the pharmacological action, antibacterial action, anti-inflammatory action, blood pressure lowering, hemostatic action, liver treatment action, antipyretic action, antiemetic action, and diuretic action have been reported. Major chemical components include forsythol, sterol compounds, saponin, and oleanolic acid.

The Dogo hot spring water and the fermented herbal medicine extract are preferably mixed in a weight ratio of 6:4 to 8:2, and more preferably in a weight ratio of 6.5:3.5 to 7.5:2.5. If the content is out of the above range, the skin whitening or wrinkle improvement effect may be lowered compared to the case where the above content is satisfied.

The Dogo hot spring water contains 55 to 65 mg/L of calcium (Ca), 25 to 35 mg/L of sodium (Na), 10 to 20 mg/L of silicon (Si), 0.001 to 0.05 mg/L of lithium (Li) and strontium ( Sr) characterized in that it comprises 1.0 to 2.0 mg / L.

The cosmetic composition of the present invention is characterized in that it is for skin whitening or wrinkle improvement.

The term "extract" used herein includes not only a crude extract obtained by treating an extraction solvent, but also a processed product of the extract. For example, the extract can be prepared in a powder state by additional processes such as vacuum distillation and freeze drying or spray drying.

In addition, the fermented herbal medicine extract including Mokdanpi, Gyejochuk, and Yeongyo of the present invention has the meaning of including formulated processed products, such as fermented herbal medicine powder, in a broad sense. Although the experiment was conducted with herbal medicines in the present invention, it would be expected by those skilled in the art that the desired effect could be achieved even in the same form as the herbal medicine processed product.

On the other hand, in the present specification, the term "contained as an active ingredient" means that it includes a sufficient amount to achieve the efficacy or activity of ingredients including Dogo hot spring water and herbal fermented extract. For example, it is used at a concentration of 10 to 1500 μg/ml, preferably 100 to 1000 μg/ml, including the Dogo hot spring water and the fermented herbal medicine extract. Dogo hot spring water and fermented herbal medicinal extract are natural products and do not have side effects on the human body even when applied to the skin in excess.

The term "skin whitening" used in the present invention is to whiten the skin, and the skin color of a person is determined by the concentration and distribution of melanin inside the skin. In addition to genetic factors, solar ultraviolet radiation, fatigue, stress, etc. are also affected by environmental or physiological conditions. Melanin is synthesized in melanocytes in the epidermal layer of the skin. An enzyme called tyrosinase acts on tyrosine, a kind of amino acid, in melanosomes, organelles in melanocytes, to produce DOPA and dopaquinone. ) and then produced through a non-enzymatic oxidation reaction. When such melanin synthesis occurs excessively in the skin, the skin tone is darkened, and spots, freckles, and the like are generated. Therefore, if the synthesis of melanin pigment is inhibited by inhibiting the activity of tyrosinase in the skin, skin whitening can be realized by brightening the skin tone, as well as skin hyperpigmentation such as spots and freckles caused by ultraviolet rays, hormones, and genetic causes. It can improve depression.

In addition, the term "wrinkle" in the present invention refers to fine lines caused by skin deterioration, which may be caused by genes, reduction of collagen present in the skin dermis, external environment, and the like. In the present invention, "wrinkle improvement" refers to inhibiting or inhibiting the formation of wrinkles on the skin, or alleviating wrinkles already formed.

The main function of skin fibroblasts is to regenerate damaged skin tissue through extracellular matrix synthesis and proliferation. Extrinsic aging factors such as photoaging or endogenous aging factors such as accumulation of free oxygen damage and cell aging due to telomere disruption They lower the physiological activity of dermal fibroblasts in the dermal layer. Type I collagen, which accounts for more than 95% of skin extracellular matrix and plays a very important part in the physiological activity of fibroblasts through interaction and signal exchange with adhesion molecules and cytoskeleton of cells When the synthesis of collagen is reduced, the amount of collagen in the skin tissue is reduced, and as a result, the surface tension is reduced, resulting in a decrease in skin elasticity and formation of skin wrinkles, as well as a decrease in physiologically active functions including cell proliferation and regeneration. cause a vicious cycle of

Therefore, collagen of dermal fibroblasts can be directly related to skin regeneration, skin elasticity, skin wrinkle formation, and tissue repair or regeneration upon skin damage. That is, when the synthesis of collagen or procollagen in skin fibroblasts is promoted or the synthesis of matrix metallo-proteinase (hereinafter referred to as 'MMP') that degrades collagen is inhibited, skin elasticity is improved, skin regeneration, skin wrinkle improvement, wound healing, repair and regeneration of damaged skin tissue; And effects such as skin aging prevention can be obtained.

The cosmetic composition of the present invention is characterized by increasing the expression of type I procollagen protein and suppressing the expression of MMP-1 protein. Specifically, the cosmetic composition of the present invention suppresses the expression of matrix metallo-proteinase, which promotes collagen degradation, while increasing the production of type I procollagen, thereby increasing skin elasticity and regenerating skin. , skin wrinkle improvement, wound healing, repair and regeneration of damaged skin tissue; And it may have effects such as skin aging prevention. That is, it may have an effect of preventing or improving skin wrinkles.

In addition, the cosmetic composition of the present invention may further include water-soluble vitamins, fat-soluble vitamins, high-molecular peptides, high-molecular polysaccharides, sphingolipids, and seaweed extracts.

Any water-soluble vitamin may be used as long as it can be formulated into cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H and the like, and their salts (thiamin hydrochloride, ascorbic acid sodium salt, etc.) or derivatives (ascorbic acid-2-phosphate sodium salt, ascorbic acid-2-phosphate magnesium salt, etc.) can also be used in the present invention. Included in water-soluble vitamins. The water-soluble vitamin can be obtained by conventional methods such as microbial transformation, microbial culture purification, enzymatic or chemical synthesis.

The fat-soluble vitamin may be any substance as long as it can be formulated into cosmetics, but preferably includes vitamin A, carotene, vitamin D2, vitamin D3, vitamin E, etc., and their derivatives (ascorbic acid palmitate, ascorbic acid stearate). Bean, ascorbine dipalmitate, dl-alpha tocopherol acetate, dl-alpha tocopherol nicotinate, vitamin E, DL-pantothenyl alcohol, D-pantothenyl alcohol, pantothenyl ethyl ether, etc.) are also included in the fat-soluble vitamins used in the present invention. do.

The polymer peptide may be anything as long as it can be incorporated into cosmetics, but preferably includes collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin, and the like. The polymeric peptide can be purified and obtained by a conventional method such as a microbial culture solution purification method, an enzyme method, or a chemical synthesis method, or can be used after being purified from a natural product such as pig or cow dermis or silkworm silk fiber.

The polymeric polysaccharide may be anything as long as it can be formulated into cosmetics, but preferably includes hydroxyethyl cellulose, xanthan gum, chondroitin sulfate or a salt thereof (sodium salt, etc.). For example, chondroitin sulfate or a salt thereof can be used after being purified from mammals or fish.

The sphingolipid may be any material as long as it can be formulated into cosmetics, but preferably includes ceramide, phytosphingosine, sphingosaccharide lipid, and the like. The sphingolipids can be purified by conventional methods or obtained by chemical synthesis from mammals, fish, shellfish, yeast or plants.

The seaweed extract may be anything as long as it can be formulated into cosmetics, but preferably brown algae extract, red algae extract, green algae extract, etc., and also calrageenan, arginic acid, and arginic acid purified from these seaweed extracts Sodium, potassium alginate, etc. are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained by purifying from seaweed by a conventional method.

In the cosmetic composition of the present invention, in addition to the above essential components, other ingredients commonly used in cosmetics may be mixed as necessary.

Other ingredients that may be added include fats and oils, humectants, emollients, ultraviolet absorbers, pH adjusters, fragrances, blood circulation promoters, cooling agents, antiperspirants, and purified water.

Examples of the oil and fat component include ester oil, hydrocarbon oil, silicone oil, fluorine oil, animal fat and vegetable oil.

Examples of the ester-based oils include tri-2-ethylhexanoate glyceryl, 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isostearate isocetyl, Butyl stearate, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, diethyl sebacate, Diisopropyl adipate, isoalkyl neopentanoate, tri(capryl, capric acid) glyceryl, trimethylolpropane tri2-ethylhexanoate, trimethylolpropane triisostearate, tetraethyl 2-ethylhexanoate pentaelli Slitol, cetyl caprylate, decyl laurate, hexyl laurate, decyl myristate, myristyl myristate, cetyl myristate, stearyl stearate, decyl oleate, cetyl ricinooleate, lauric acid sostearyl, isotridecyl myristate, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyldodecyl oleate, octyldodecyl linoleate, isopropyl isostearate, 2-ethylhexanoate Tostearyl, 2-ethylstearyl hexanoate, hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicaprate, propylene glycol dicaprylate, neopentyl glycol dicaprate, neopentyl dioctanoate Glycol, glyceryl tricaprylate, glyceryl triundecylate, glyceryl triisopalmitate, glyceryl triisostearate, octyldodecyl neopentanoate, isostearyl octanoate, octyl isononanoate, hexyl neodecanoate Decyl, octyldodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, octyldecyl isostearate, polyglycerin oleate, polyglycerin isostearate, triisocetyl citrate, triisoalkyl citrate, Triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyldecyl lactate, triethyl citrate, acetyl triethyl citrate, acetyl tributyl citrate, trioctyl citrate, diisostearyl malate, hydroxystearate 2- Ethylhexyl, di2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, dioctyl sebacate, cholesteryl stearate, isostear Cholesteryl acid, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, phyteryl isostearate, physteryl oleate, isocetyl 12-stealoylhydroxystearate, 12 - Ester systems, such as stearyl stealoyl hydroxy stearate and isostearyl 12-stealoyl hydroxy stearate, etc. are mentioned.

Examples of the hydrocarbon-based fats and oils include hydrocarbon-based fats and oils such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybuden, microcrystalline wax, and vaseline.

Examples of the silicone-based oil include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane methylcetyloxysiloxane copolymer, dimethylsiloxane methylstealoxysiloxane copolymer, alkyl Modified silicone oil, amino modified silicone oil, etc. are mentioned.

Examples of the fluorine-based fats and oils include perfluoropolyether and the like.

The animal or vegetable oils include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, soybean oil, corn oil, rapeseed oil, apricot oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil, cottonseed oil, palm oil, Kukui nut oil, wheat germ oil, rice germ oil, shea butter, moonshine colostrum, marker daemia nut oil, egg yolk oil, beef tallow, horse oil, mink oil, orange raffia oil, jojoba oil, candelilla wax, carnaba wax, liquid lanol , animal or vegetable fats and oils such as hydrogenated castor oil.

Examples of the moisturizer include water-soluble low-molecular moisturizers, fat-soluble molecular moisturizers, water-soluble polymers, and oil-soluble polymers.

The water-soluble low-molecular moisturizers include serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylate, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = 2 or more), Polypropylene glycol (polymerization degree n = 2 or more), polyglycerol B (polymerization degree n = 2 or more), lactic acid, lactate, etc. are mentioned.

Examples of the fat-soluble low-molecular moisturizers include cholesterol and cholesterol esters.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyaspartate, tragacanth, xanthan gum, methyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, carboxymethyl cellulose, water-soluble chitin, chitosan, dextrin, etc. can be heard

Examples of the fat-soluble polymer include polyvinylpyrrolidone eicosene copolymer, polyvinylpyrrolidone hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, polymeric silicone, and the like.

Examples of the emollient agent include long-chain acyl glutamic acid cholesteryl ester, hydroxystearic acid cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid, and lanolin fatty acid cholesteryl ester.

The ultraviolet absorber includes para-aminobenzoic acid, para-aminobenzoic acid ethyl, para-aminobenzoic acid amyl, para-aminobenzoic acid octyl, salicylic ethylene glycol, salicylic acid phenyl, salicylic acid octyl, salicylic acid benzyl, salicylic acid butylphenyl, salicylic acid homomentyl, Benzyl cinnamate, paramethoxycinnamic acid-2-ethoxyethyl, paramethoxycinnamic acid octyl, diparamethoxycinnamic acid mono-2-ethylhexane glyceryl, paramethoxycinnamic acid isopropyl, diisopropyl-diisopropylcinnamic acid ester mixture , urocanic acid, ethyl urocanate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and its salts, dihydroxymethoxybenzophenone, dihydroxymethoxybenzophenone sodium disulfonate, dihydroxy Benzophenone, tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino-p-(carbo-2'-ethylhexyl-1'-oxy) -1,3,5-triazine, 2-(2-hydroxy-5-methylphenyl) benzotriazole, etc. are mentioned.

Examples of the pH adjusting agent include citric acid, sodium citrate, malic acid, sodium malate, fumalic acid, sodium fumalate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogenphosphate, and the like.

Examples of the pH adjusting agent include citric acid, sodium citrate, malic acid, sodium malate, fumalic acid, sodium fumalate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogenphosphate, and the like.

Cosmetics prepared by containing the cosmetic composition of the present invention may take the form of solutions, emulsions, viscous mixtures, and the like.

In addition, the ingredients included in the cosmetic composition of the present invention may include ingredients commonly used in cosmetic compositions in addition to the above ingredients as active ingredients, for example, conventional adjuvants such as stabilizers, pigments and natural flavors, and A carrier may be further included.

The cosmetic composition of the present invention can be used in various ways such as cosmetics or facial cleansers having skin whitening or wrinkle improvement effects.

Products to which the cosmetic composition of the present invention can be added include, for example, BB cream, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrient lotion, massage cream, nutrient cream, and ultraviolet rays. There are cosmetics such as blocking cream, moisture cream, hand cream, foundation, essence, nutrient essence, and mask pack, as well as soap, cleansing foam, cleansing lotion, cleansing cream, body lotion, and body cleanser.

When the formulation of the present invention is a paste, cream or gel, animal fiber, vegetable fiber, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. can

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane , butane or a propellant such as dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butyl glycol oil, fatty acid esters of glycerol, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar or tracanth and the like may be used.

Hereinafter, preferred embodiments are presented to aid understanding of the present invention, but the following examples are merely illustrative of the present invention, and various changes and modifications are possible within the scope and spirit of the present invention. It is obvious to those skilled in the art, It goes without saying that these variations and modifications fall within the scope of the appended claims.

[Example]

Preparation Example 1: Moutanpi fermented extract

Black yeast ( Aureobasidium pullulans ) was cultured using YM (Yeast extract / Malt extract, Difco, Co., USA) medium at 25 ° C., and subcultured at 4-week intervals. After inoculating a seed culture of black yeast ( Aureobasidium pullulans SM-2001) passaged in a liquid medium, it was cultured for 3 days in a rotary shaker at 25° C. and 130 rpm and used as a liquid spawn.

On the other hand, after mixing the mudanpi powder and sugar at a weight ratio of 20: 1, put them in a culture container, sterilize at 120 ° C. for 20 minutes, and then place at room temperature to prepare a medium. The black yeast liquid spawn was inoculated to 3% (v / v) in a medium containing the prepared moutan bark, and cultured for 30 hours at 25 ° C. temperature conditions in an incubator with a relative humidity of 80%. The culture was extracted in 10-fold volume of 90 ° C. hot water for 2 hours, centrifuged, the supernatant was taken, concentrated under reduced pressure, and powdered to prepare a fermented extract powder of Mokdanpi.

Preparation Example 2: Gyejochu fermented extract

A fermented fermented extract was prepared in the same conditions as in Preparation Example 1, except that the gray axis was used instead of the moutan bark.

Preparation Example 3: Yeongyo fermented extract

A fermented yeast extract was prepared in the same conditions as in Preparation Example 1, except that Yeongyo was used instead of Mokdanpi.

Example 1: Dogo hot spring water + 3 types of fermented herbal medicine

Three types of fermented herbal medicines were prepared by mixing the fermented extract of Moutan bark of Preparation Example 1, the fermented extract of Gyejochuk of Preparation Example 2, and the fermented Yeongyo extract of Preparation Example 3 in a ratio of 1:1:1, and Dogo hot spring water and the three types of fermented herbal medicines. Dogo hot spring water/fermented herbal medicine complex was prepared by mixing at a weight ratio of 7:3.

Comparative Example 1: Asan hot spring water + 3 kinds of fermented herbal medicine

A composite of Asan hot spring water/fermented herbal medicine was prepared under the same conditions as in Example 1, except that Onyang hot spring water was used instead of Dogo hot spring water.

Comparative Example 2: Onyang hot spring water + 3 types of fermented herbal medicine

An Onyang hot spring water/fermented herbal medicine composite was prepared under the same conditions as in Example 1, except that Onyang hot spring water was used instead of Dogo hot spring water.

Comparative Example 3: Dogo hot spring water + 3 kinds of herbal medicine

Instead of the three kinds of fermented herbal medicines in which the fermented extract of Moutan bark in Preparation Example 1, the fermented extract in Preparation Example 2, and the fermented extract in Preparation Example 3 were mixed 1: 1: 1, the extracts of Moutan bark, Gyejo axis and Yeongyo hot water were mixed 1: A Dogo hot spring water/herbal medicine complex was prepared under the same conditions as in Example 1, except that the mixture was mixed at a ratio of 1:1.

Here, the hot water extract is extracted with hot water at 100 ° C., which is 10 times the weight of each herbal medicine, filtered with a non-woven fabric to remove impurities, concentrated under reduced pressure, dried and powdered.

Comparative Example 4: Dogo hot spring water + fermented moutan skin + fermented gray scale

Dogo hot spring water/fermentation under the same conditions as in Example 1, except that the fermented herbal extract of Moutan bark of Preparation Example 1 and the fermented extract of Gyejochuk of Preparation Example 2 were mixed in a ratio of 1:1 instead of the three kinds of fermented herbal medicine. A herbal medicine complex was prepared.

Comparative Example 5: Dogo Hot Spring Water + Fermented Moutan Skin + Fermented Yeongyo

Dogo hot spring water/fermented herbal medicine under the same conditions as in Example 1, except that the fermented herbal extract of Mudanpi of Preparation Example 1 and the fermented extract of Yeongyo of Preparation Example 3 were mixed in a 1:1 ratio instead of the three kinds of fermented herbal medicine. A composite was prepared.

Comparative Example 6: Dogo hot spring water + fermented gradation axis + fermented Yeongyo

Dogo hot spring water/fermentation under the same conditions as in Example 1, except that instead of the three kinds of fermented herbal medicines, two kinds of fermented herbal medicines in which the fermented fermented extract of Gyejochuk of Preparation Example 2 and the fermented extract of Yeongyo in Preparation Example 3 were mixed at a ratio of 1:1 were used. A herbal medicine complex was prepared.

Comparative Example 7: Pure Dogo Hot Spring Water

Pure Dogo hot spring water was used without mixing with three types of fermented herbal medicines.

Comparative Example 8: 3 kinds of fermented herbal medicine

Three types of fermented herbal medicines were used, which were mixed with the fermented extract of Mudanpi of Preparation Example 1, the fermented extract of Gyejochuk of Preparation Example 2, and the fermented Yeongyo extract of Preparation Example 3 at a ratio of 1:1:1 without mixing with Dogo hot spring water.

The components of Example 1 and Comparative Examples 1 to 8 are summarized in Table 1 below.

division spa herbal medicine Whether or not herbal medicine is fermented Example 1 Dogo hot spring water Moktan Skin + Gyejochuk + Yeongyo fermentation Comparative Example 1 Asan hot spring water Moktan Skin + Gyejochuk + Yeongyo fermentation Comparative Example 2 Onyang hot spring water Moktan Skin + Gyejochuk + Yeongyo fermentation Comparative Example 3 Dogo hot spring water Moktan Skin + Gyejochuk + Yeongyo No fermentation Comparative Example 4 Dogo hot spring water Mokdan skin + gradation axis fermentation Comparative Example 5 Dogo hot spring water Mokdan skin + Yeongyo fermentation Comparative Example 6 Dogo hot spring water Gradation axis + year school fermentation Comparative Example 7 Dogo hot spring water doesn't exist - Comparative Example 8 doesn't exist 3 types complex fermentation

For reference, the results of comparing the mineral components and contents of Dogo hot spring water used as a medium component in Examples, which are the three major hot spring waters distributed in the Asan area, and Asan hot spring water and Onyang hot spring water used in Comparative Examples are shown in Table 2 below . The results in Table 2 are quoted from a known project report related to “Development of skin-improving cosmetics using hot spring water in Chungcheongnam-do.”

[Experimental example]

All statistical analyzes were performed using SPSS version 18.0 (IBM, Chicago, IL, USA). A one-way ANOVA with Duncan's post-hoc test was used to determine the difference (p <0.05) in the mean value between the experimental groups. Data from each experiment were expressed as mean ± standard deviation (SD).

Experimental Example 1: Cytotoxicity assay

Cytotoxicity was measured using the MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) reduction method. The cultured HaCaT cells were mixed well in DMEM medium, adjusted to 1x10 ⁵ cells/mL, and then 1 mL of each prepared cell was added to a 24-well culture plate and cultured for 3 days. Samples of Example 1 and Comparative Examples 1 to 3 (0, 25, 50, 100, 300, 400, 500, 1000 μg / ㎖), the culture was further cultured for 24 hours, and the viability of the cultured cells was measured using the MTT reduction method.

After adding about 1/10 of the volume of the growth medium to each well, the MTT solution (5 mg/ml) was further incubated at 37 °C for 4 hours. Thereafter, the medium was removed while being careful not to lose the formazan produced by reducing MTT, and after leaving the medium at room temperature for 30 minutes to completely remove the remaining medium, the sample was dissolved using DMSO and then 570 nm The absorbance was measured at. Cell viability was calculated according to Equation 1 below, and the results are shown in Table 3.

[Equation 1]

Cell viability (%) = [absorbance of sample treatment group/absorbance of control group] x 100

division Concentration (μg/ml) 10 100 untreated 100±1.1 Example 1 98.6±1.1 94.3±0.7 Comparative Example 1 98.9±0.3 95.1±0.5 Comparative Example 2 96.1±2.4 91.2±1.2 Comparative Example 3 97.7±0.8 93.8±1.1

According to this, it was confirmed that not only the components prepared according to Example 1 of the present invention, but also the components of Comparative Examples 1 to 3 can be safely used on human skin. Since the cosmetic compositions of Comparative Examples 4 to 8 are included in the components of Example 1 or other Comparative Examples, separate cytotoxicity was not performed.

Experimental Example 2: MMP-1 synthesis inhibitory effect analysis

CCD-986sk was cultured in a 35 mm dish at a concentration of 1.5X10 ⁵ cells/ml until reaching about 80% confluency. After removing the original medium, washing with PBS to remove the serum component in the medium, UVA (6.3J/cm2) was irradiated for 24 hours using a UV light source (Coralife, 35W, UV) equipped with a filter. . After UVA irradiation, the samples of Examples or Comparative Examples of the present invention were administered at concentrations of 10 and 100 μg/ml in DMEM medium without FBS, respectively, and cultured for 24 hours. The cultured medium was dispensed into a 96 well plate and reacted overnight at 4 °C. MMP-1 expression level induced by UVA irradiation was measured using the method used by Dunsmore et al.

First, the medium was washed three times with TBS (phosphate bufferedsaline + 0.05% Tween20), and then blocked with 3% PBS at 37 °C for 1 hour. Thereafter, a solution diluted with the primary antibody (monoclonal anti-MMP-1 antibody) in a blocking buffer at a volume ratio of 1:3000 was added and reacted at 37° C. for 90 minutes. Thereafter, after washing with PBS, a solution diluted with a secondary antibody (alkaline phosphatase conjugated Goat anti-mouse IgG) in a blocking buffer at a volume ratio of 1: 3,000 was added and reacted at 37° C. for 90 minutes. Thereafter, after washing with PBS, alkaline phosphatase substrate solution (1 mg/ml, p-mitrophenylphosphate in diethanolamine buffer) was added, followed by reaction at room temperature for 30 minutes, and then the reaction was stopped with 3N NaOH. Next, absorbance was measured at 405 nm using a microplate reader, and the results are shown in Table 4 below.

division Concentration (μg/ml) 10 100 untreated 8752±55 Example 1 4830±88 3938±98 Comparative Example 1 5240±110 4206±98 Comparative Example 2 5015±58 4325±95 Comparative Example 3 7400±45 6324±35 Comparative Example 4 5948±58 5120±29 Comparative Example 5 6840±75 6002±134 Comparative Example 6 6257±66 5802±76 Comparative Example 7 7723±56 6327±35 Comparative Example 8 7523±98 6750±112

According to this, Example 1, which is a complex of three kinds of fermented herbal medicines, Mokdanpi, Gyejochuk, and Yeongyo, and Dogo hot spring water, was measured to have a very high inhibitory effect on the synthesis of MMP-1 protein compared to other experimental groups, and while including three kinds of fermented herbal medicines, Dogo In the case of Comparative Examples 1 and 2 containing Asan hot spring water or hot spring water rather than hot spring water, the effect of inhibiting the synthesis of MMP-1 protein was higher than that of other comparative examples, but relatively low compared to Example 1. showed up In addition, in the case of non-fermentation or using two kinds of herbal medicines, the synthesis inhibitory effect of MMP-1 protein was relatively low.

Experimental Example 3: Collagen synthesis promotion effect analysis

In order to confirm the skin wrinkle improvement effect of the cosmetic compositions of Examples and Comparative Examples, the amount of protein collagen produced to maintain skin elasticity was measured. Fibroblasts were dispensed at a density of 1X10 ⁴ cells/well in a 48 well plate, cultured for 24 hours in DMEM medium containing 10% FBS, and samples of Examples or Comparative Examples were added at 50 and 100 μg/ml, and no Incubated for an additional 24 hours in serum medium. After incubation, the supernatant of each well was collected and the amount of procollagen type I C-peptide (PICP) was measured using the procollagen type I C-peptide EIA kit (MK101, Takara, Japan) and converted into ng/ml. , The amount of collagen synthesized thereby was measured, and the results are shown in Table 5.

division Concentration (μg/ml) 50 100 untreated 41.0±0.8 Example 1 81.2±0.7 89.8±0.5 Comparative Example 1 77.8±0.3 82.9±0.8 Comparative Example 2 79.1±0.3 85.2±0.2 Comparative Example 3 49.5±0.8 57.6±0.9 Comparative Example 4 63.6±1.2 75.0±1.4 Comparative Example 5 58.5±0.7 65.7±0.6 Comparative Example 6 50.6±0.2 60.8±0.1 Comparative Example 7 45.8±0.5 50.2±0.2 Comparative Example 8 52.7±0.8 63.4±0.7

According to this, Comparative Examples 1 and 2, which are a composite of Dogo hot spring water/3 types of fermented herbal medicines and 3 types of fermented herbal medicines and Asan hot spring water or Onyang hot spring water prepared according to Example 1 of the present invention, are not in the untreated group or other comparative examples. It was found that the effect of promoting collagen production was excellent compared to that of Example 1 containing Dogo hot spring water. On the other hand, Comparative Examples 4 to 6 including two kinds of fermented herbal medicines and Comparative Example 3 including three kinds of non-fermented herbal medicines had a relatively low collagen production promoting effect. In addition, Comparative Example 7 containing only Dogo hot spring water showed the lowest collagen production promoting effect.

Experimental Example 4: Analysis of tyrosinase inhibitory activity

The tyrosinase inhibitory ability of the fermented products of Examples and Comparative Examples was measured by the method of Yagi et al. (1986). Melanin exists in the basal layer of the epidermis, and melanin synthesis is regulated by tyrosinase in melanocyte intracellular melanocytes. Therefore, inhibiting tyrosinase activity can reduce melanin synthesis and pigmentation. 0.2 ml of 110 Unit/ml mushroom tyrosinase was added to a mixture containing 0.2 ml of a substrate solution in which 10 mM L-DOPA was dissolved in 0.5 ml of 1.15M sodium phosphate buffer at pH 6.8 and 0.1 ml of a sample solution in a test tube. After reacting at 25 ° C. for 2 minutes, the absorbance of DOPA chrome generated in the reaction solution was measured at 490 nm using a microplate reader. The tyrosinase inhibitory activity was expressed as the rate of decrease in absorbance of the sample solution added and non-added groups, and the results are shown in Table 6 below.

division Treatment concentration (% (w/v)) 50 100 Example 1 7.2±0.4 12.8±0.2 Comparative Example 1 6.0±0.2 10.9±0.7 Comparative Example 2 5.9±0.3 9.2±0.3 Comparative Example 3 3.8±0.4 7.7±0.3 Comparative Example 4 5.2±0.5 7.8±0.2 Comparative Example 5 4.1±0.1 7.1±1.0 Comparative Example 6 3.8±0.2 7.6±0.1 Comparative Example 7 2.7±0.4 5.7±0.3 Comparative Example 8 2.9±0.7 6.0±0.4

According to this, Dogo hot spring water/3 kinds of fermented herbal medicines of Example 1 of the present invention showed the highest tyrosinase inhibitory activity, Asan hot spring water/3 kinds of fermented herbal medicines of Comparative Example 1, and Onyang hot spring water/of Comparative Example 2 The three kinds of fermented herbal medicines also showed relatively high tyrosinase inhibitory activity, although slightly lower than that of Example 1. On the other hand, Comparative Example 7 containing only pure hot spring water and Comparative Example 8 containing only three types of fermented herbal medicines without containing hot spring water showed the lowest tyrosinase inhibitory activity, even when two or less types of fermented herbal medicines were included. showed low tyrosinase inhibitory activity.

Experimental Example 5: Analysis of melanin production inhibitory ability

Mouse-derived melanocyte B16F1 cells produce melanin, and melanin is biosynthesized in the melanosome, an organelle within the melanocyte present in the basal layer of the epidermis, and the amount of melanin produced can be compared by measuring the absorbance in the visible light range. After inoculation of B16F1 cells, the sample was treated for 3 days, the medium was removed, the cells were washed with PBS, 1 ml of 1 N NaOH was added to each well, and the melanin component was dissolved by stirring to melt the cell membrane, and the visible light range The amount of melanin produced was measured by measuring the absorbance at 400 nm, which is the shortest wavelength in the blue visible ray region.

The amount of melanin was measured by a method of expressing the absorbance per unit cell number (10 ⁶ cell), and the relative amount of melanin production with respect to the control group (untreated group) was calculated as an inhibition rate (%), and the results are summarized in Table 7.

Classification (unit: %) Concentration (μg/ml) 50 100 untreated 39.8±0.5 Example 1 69.3±0.4 73.5±1.4 Comparative Example 1 63.5±0.9 70.7±0.2 Comparative Example 2 64.0±1.1 69.9±0.7 Comparative Example 3 43.7±1.3 48.7±0.8 Comparative Example 4 52.4±0.8 62.1±0.4 Comparative Example 5 36.2±0.9 45.7±0.3 Comparative Example 6 44.2±1.4 49.6±0.5 Comparative Example 7 43.5±0.5 48.6±1.7 Comparative Example 8 42.5±0.8 50.3±0.9

According to this, the Dogo hot spring water / 3 types of fermented herbal medicine complex of Example 1 was found to have the best melanin production inhibitory ability, and even when using Asan hot spring water or Onyang hot spring water, not Dogo hot spring water, while using 3 types of fermented herbal medicines, Example 1 It was lower than 1, but showed a relatively high melanin production inhibitory ability compared to other comparative examples. On the other hand, when only 3 kinds of fermented herbal medicines were included, when only Dogo hot spring water was included, or when 2 or less kinds of fermented herbal medicines were used, the ability to inhibit melanin production was low.

Formulation examples of cosmetic compositions suitable for application of the present invention are presented.

Formulation Example 1: Lotion

Formulation examples of lotion using the cosmetic composition of Example 1 are shown in Table 8 below.

ingredient Content (% by weight) Composition of Example 1 5.0 glycerin 6.0 1,3-butylene glycol 3.0 PEG 1500 1.0 allantoin 0.1 DL-panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 sodium hyaluronate 5.0 ethanol 10.0 Polysorbate 20 0.2 preservatives, fragrances, colors a very small amount Distilled water balance Sum 100

Formulation Example 2: Lotion

Formulation examples of lotions using the cosmetic composition of Example 1 are shown in Table 9 below.

ingredient Content (% by weight) Composition of Example 1 3.0 propylene glycol 6.0 glycerin 4.0 triethanolamine 1.2 Tocopheryl Acetate 3.0 liquid paraffin 5.0 squalane 3.0 macadamia nut oil 2.0 polysorbate 60 1.5 Sorbitan sesquioleate 1.0 carboxyvinyl polymer 1.0 preservatives, fragrances, colors a very small amount Distilled water balance Sum 100

Formulation Example 3: BB Cream

Formulation examples of BB cream using the cosmetic composition of Example 1 are shown in Table 10 below.

ingredient Content (% by weight) Composition of Example 1 1.0 Pro-type glycerin monostearate 1.5 cetearyl alcohol 1.5 stearic acid 1.0 polysorbate 60 1.5 Sorbitan Stearate 0.6 isostearyl isostearate 5.0 squalane 5.0 mineral oil 35.0 dimethicone 0.5 Hydroxyethyl Cellulose 0.12 glycerin 6.0 triethanolamine 0.7 preservatives, fragrances, colors a very small amount Distilled water balance Sum 100

Formulation Example 4: Essence

Formulation examples of essence using the cosmetic composition of Example 1 are shown in Table 11 below.

ingredient Content (% by weight) Composition of Example 1 1.5 glycerin 10.0 betaine 5.0 PEG 1500 2.0 allantoin 0.1 DL-panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 Hydroxyethyl Cellulose 0.1 sodium hyaluronate 8.0 carboxyvinyl polymer 0.2 triethanolamine 0.18 Octyldodecanol 0.3 Octyldodeces-16 0.4 ethanol 6.0 preservatives, fragrances, colors a very small amount Distilled water balance Sum 100

Formulation Example 5: Emulsion for mask pack

Formulation examples of emulsions for mask packs using the cosmetic composition of Example 1 are shown in Table 12 below.

ingredient Content (% by weight) Composition of Example 1 1.0 polyvinyl alcohol 15.0 cellulose gum 0.15 glycerin 3.0 PEG 1500 2.0 cyclodextrin 0.15 DL-panthenol 0.4 allantoin 0.1 Monoammonium glycyrrhizinate 0.3 nicotinamide 0.5 ethanol 6.0 PEG 40 hydrogenated castor oil 0.3 preservatives, fragrances, colors a very small amount Distilled water balance Sum 100

Although the embodiments of the present invention have been described above, those skilled in the art can add, change, delete, or add components within the scope not departing from the spirit of the present invention described in the claims. The present invention can be variously modified and changed by the like, and this will also be said to be included within the scope of the present invention.

Claims (8)

Dogo hot spring water; and
A cosmetic composition comprising, as an active ingredient, at least one fermented herbal medicine extract selected from Moutanpi, Gyejochuk, and Yeongyo. According to claim 1,
The fermented extract of the herbal medicine is a cosmetic composition, characterized in that the extract of the fermented black yeast ( Aureobasidium pullulans ). According to claim 2,
The extract is a cosmetic composition, characterized in that extracted by water, alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof. According to claim 1,
The herbal medicine fermented extract is a cosmetic composition, characterized in that a mixture of moutanpi fermented extract, gyaejochu fermented extract and annual fermented extract. According to claim 4,
The fermented extract of the herbal medicine is a cosmetic composition, characterized in that it comprises 50 to 150 parts by weight of the fermented extract of Gyejochu, and 50 to 150 parts by weight of the fermented extract of Yeongyo, based on 100 parts by weight of the fermented extract of Mudanpi. According to claim 1,
The cosmetic composition, characterized in that the Dogo hot spring water and the fermented herbal medicine extract are mixed in a weight ratio of 6:4 to 8:2. According to claim 1,
The Dogo hot spring water contains 55 to 65 mg/L of calcium (Ca), 25 to 35 mg/L of sodium (Na), 10 to 20 mg/L of silicon (Si), 0.001 to 0.05 mg/L of lithium (Li) and strontium ( Sr) A cosmetic composition comprising 1.0 to 2.0 mg/L. According to claim 1,
The cosmetic composition is a cosmetic composition, characterized in that for skin whitening or wrinkle improvement.