KR20220169190A - 수박의 여교배 세대단축 육종을 위한 단일염기 다형성 마커세트 및 이의 용도 - Google Patents

수박의 여교배 세대단축 육종을 위한 단일염기 다형성 마커세트 및 이의 용도 Download PDF

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KR20220169190A
KR20220169190A KR1020210079294A KR20210079294A KR20220169190A KR 20220169190 A KR20220169190 A KR 20220169190A KR 1020210079294 A KR1020210079294 A KR 1020210079294A KR 20210079294 A KR20210079294 A KR 20210079294A KR 20220169190 A KR20220169190 A KR 20220169190A
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citrullus lanatus
watermelon
single nucleotide
fastq
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한고은
이은수
김도선
이혜은
이옥진
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대한민국(농촌진흥청장)
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Abstract

본 발명은 수박의 여교배 세대단축 육종을 위한 단일염기 다형성 마커세트 및 이의 용도에 관한 것으로, 분자표지 이용 여교배(MAB) 또는 품종 판단 등에 이용될 수 있고, 수박 형질의 유사성을 판단하는데 필요한 시간과 비용을 절감하거나 판단의 정확도를 높일 수 있다.

Description

수박의 여교배 세대단축 육종을 위한 단일염기 다형성 마커세트 및 이의 용도 {MARKERS FOR WATERMELON (CITRULLUS LANATUS) GENERATION SHORTENING OF BACKCROSS BREEDING AND USES THEREOF}
본 발명은 수박의 여교배 세대단축 육종을 위한 단일염기 다형성 마커세트 및 이의 용도에 관한 것이다.
여교배(backcross) 방법은 특정한 품종의 유용한 형질을 실용성이 우수한 다른 품종에 도입하기 위해 사용되는 육종법으로서, 도입하고자 하는 유용형질을 갖는 품종을 도입친, 실용성이 우수한 품종을 반복친으로 하여 교배함으로써 최종적으로 반복친과 유사하면서도 도입친의 유용형질을 갖는 개체를 선별하는 과정으로 이루어진다.
종래에는 원하는 형질을 갖는 개체를 선별하기 위해서 여교배한 개체의 표현형을 확인하였다. 그러나 개체를 성장시켜 표현형을 육안으로 확인하여 선별하고, 원하는 형질의 개체를 얻을 때까지 반복하여 세대진전을 시켰기 때문에 매우 오랜 시간이 소요되는 문제점이 있었다.
최근 이러한 문제를 해소하기 위해, 개체가 성장하기 전 단계에서 유전자를 확인함으로써 조기에 원하는 개체를 선별할 수 있는 분자표지를 이용한 육종방법이 개발되고 있다. 분자표지이용 여교배(Marker assisted Backcrossing, MABC)는 도입친의 목표 유전자를 보유하되, 목표 유전자를 제외한 다른 부분들이 반복친의 유전자 구성을 갖는 여교배 자손을 선별함으로써 이루어질 수 있다.
이를 위해서는 유전체 전체적으로 고르게 분포하고 있는 분자표지를 확보해야 하고, 반복친과 여교배 자손의 분자표지를 비교하여 반복친의 유사성이 높은 여교배 자손을 선발할 수 있어야 하며, 비교대상 분자표지는 형질과 관련성이 있어야 한다. SNP는 탐색과 분석에 이르는 전 과정이 자동화될 수 있는 분자표지로서, 유전형 분석에 있어 다른 분자 표지보다 많은 장점을 가지고 있다.
다양한 작물에 대해 분자표지이용 여교배를 위한 SNP 세트가 개발되고 있으나, 수박 (Citrullus lanatus)에 대해서는 여교배 자손 선별에 실질적으로 도움을 줄 수 있는 분자표지가 알려져 있지 않다.
1. KR 10-2125528B1
본 발명의 일 목적은 서열번호 1 내지 341의 염기서열에서 각 염기서열의 61번째 염기에 위치한 단일염기다형성 (single nucleotide polymorphism, SNP) 전부를 검출하기 위한 제제를 포함하는, 수박의 여교배 세대단축 품종 식별용 조성물을 제공하는 것이다.
본 발명의 다른 일 목적은 상기 조성물을 포함하는, 수박의 여교배 세대단축 품종 식별용 키트를 제공하는 것이다.
본 발명의 다른 일 목적은 a) 반복친 수박 시료로부터 폴리뉴클레오티드를 분리하고, 서열번호 1 내지 341번으로 표시되는 각각의 염기서열의 61번째 위치에 존재하는 단일염기다형성 전부의 유전자형을 결정하는 단계; b) 여교배 후대 수박 시료로부터 폴리뉴클레오티드를 분리하고, 상기 a)단계의 단일염기다형성의 유전자형을 결정하는 단계; c) 상기 a)단계에서 결정된 반복친의 단일염기다형성 유전자형과 상기 b)단계에서 결정된 여교배 후대의 단일염기다형성 유전자형을 비교하는 단계; 및 d) 여교배 후대 중에서 반복친과 단일염기다형성 유전자형이 일치하는 비율이 높은 여교배 후대를 선별하는 단계를 포함하는, 수박의 여교배 세대단축 후대 선별 방법을 제공하는 것이다.
본 발명의 일 양상은 서열번호 1 내지 341의 염기서열에서 각 염기서열의 61번째 염기에 위치한 단일염기다형성 (single nucleotide polymorphism, SNP) 전부를 검출하기 위한 제제를 포함하는, 수박의 여교배 세대단축 유전형 차이 식별용 조성물을 제공한다.
본 발명에 있어서 수박의 여교배 세대단축 품종 식별은 수박의 품종을 식별하는 것뿐만 아니라, 수박의 계통을 식별하는 것일 수 있다. 상기 수박의 품종간 형질 차이 식별은 수박의 형질 차이를 식별하는 것일 수 있다. 상기 계통은 동일한 품종에 속하면서도 세부적인 형질의 차이로 분류할 수 있는 개체들을 의미한다. 상기 수박의 품종간 형질 차이 식별용 조성물은 수박의 품종, 계통, 유전형, 표현형, 형질, 및 이들의 조합 중 하나 이상에 대해 식별하거나 또는 유사성을 판단하는데 이용될 수 있다. 상기 수박의 품종간 형질 차이 식별용 조성물은 단일염기다형성 부위를 포함되는 폴리뉴클레오티드를 검출 또는 증폭함으로써 선택된 단일염기다형성의 유전자형을 식별하는 것일 수 있다.
상기 서열번호 1 내지 341의 염기서열은 아래와 같다:
Marker Name Chromosome SNP position (bp) Flanking Sequence 서열번호
Cl-01-97 Cla97Chr01 97,366 AATAAAAATGGAAGGATAACTAAGTAAAACGTTGAAAGTGGGAGAAGATTTAGATTTTTT[A/T]AAAAAATACAATTAGTAAAGTGGCTGTTGATATGGGTAATTTAAAATTAAAATATAAAAT 1
Cl-01-142 Cla97Chr01 141,661 ATATTGAAGATATCAATTAATTCTTAATATCAATGTTTCTATTCGATTCACAAATAGAAT[G/T]ACATATTGATTAATATTAGATTAAAGTTGTGGGAAGTGAAATTTCAATCAATAATCAAAT 2
Cl-01-972 Cla97Chr01 972,106 TCCTTTTCAAAAAATATGACTATCATAAATAATAGTAGGAGAGATTCGAACCAGTTAACT[T/C]GTGTAACAATATCACAATTTCTATTTTGTTTTATCAAATGAACATGTGGCTGGTGTATTT 3
Cl-01-1929 Cla97Chr01 1,928,852 TTATATCATGTTTACATCAATAATATTTTGTTGCTTATTATTTTTTTTTATTTTTTATAA[T/C]TTTTTAACGATATGATAAAAATAACGAGTAACTTCTAATGTCAATGTTGAACTCATAAAA 4
Cl-01-2773 Cla97Chr01 2,772,600 TTTGAAAACTTCCATTCTAGAAAAGAGTTTAAGAAAAGAAAGGAAGAAAAAAAAACCATG[C/T]TCTTCACACAAGTTTTTTCACTTTAATTTCAATCTTTTAAAACACACCTTTGAATTTTGA 5
Cl-01-3408 Cla97Chr01 3,407,936 TTCTCTCAAAAGAATTAAATTTAGAATTTTTGTGTTGACAAACATATCCACCACCGACAA[T/A]TATATACCTACATACATACGTGTATGTATATAAATATATATATCCACACACGTGTATATA 6
Cl-01-5391 Cla97Chr01 5,391,003 GTGTCTTAATGCTCAACTTCAGTACAGAGAAAATATTTGATACCCGAATGGTGTAGCCTC[A/G]ACTAGAGAGAATCAAACTCAAGCAACTCAATTGAACTCAAAATGAAGCTATTGCTCAAAG 7
Cl-01-6299 Cla97Chr01 6,299,163 CTAAGTCATTTAAGGTTTGGAACTAGCAATTGAAAAGATTTTAGAGTGTCCTCAGGTTTC[G/A]GTATTGTTCCTCCAATGATTGTAGTACCAAGTACTTCTTGCCGAGCTCTAATACTCCTAG 8
Cl-01-6879 Cla97Chr01 6,879,427 ATGGTTATCATATTGAGACTGATATGATAAATGAGATAGAACATCTTTATATCATATCTT[T/C]TATCTCACATGAAAAACGTATATTGGAAAAGTTGCTTGCTTTATCTTCTGGATTATATTA 9
Cl-01-7211 Cla97Chr01 7,211,341 GTTTTGGACTTACTACTTGGTTTATGCTGAAGCCTATGTCTATGCTTATTGTATGGACAG[G/A]ATTATTGTATTGGTTGGTTATGGTTACTGCGACTTATTACTATGCACTTAACTTACTACT 10
Cl-01-7372 Cla97Chr01 7,371,662 TTTTTTAAAAAATAAAAAATAGTTTTTTTTGCATTGTGATATTTAGGTTAATATTTTGGG[C/T]CCAATTACTATATATTTGCAACTACCCAAAAACTTTAGTTACTACTAGCAGGGTTGCAAC 11
Cl-01-8817 Cla97Chr01 8,816,622 AATGTGCGGAGTCAACTTAATGCCATTAACAGAAAGCAAAGTGGAAGGTATGAATGTTCA[C/A]AATAGGTACAGTTGTATTTAGCCAATTTGATGTTTCTAATTGATCGTGCTTTCATCAGCT 12
Cl-01-9393 Cla97Chr01 9,393,444 ATTGATTGCATTTTCTTCAACAAAATGAGCATCTCTCCTCCAACGAATTAACTGTCTCTT[T/C]TTCAACAAACTGAGCATCTCTTCTTTAACAAATTGAACGTCTCTTCGCCATCTACAAATT 13
Cl-01-10434 Cla97Chr01 10,434,369 GCATAGCTTGATTATAGGTGAGATTGTGGCTAAGTGTTGAGCGACTTATCTTAGTTCAAG[G/A]TAAAACACTTAAGCCAAAGTGCAAGCATAGGGGGTTGGACGCATACTCTATCTAAGGTGC 14
Cl-01-12184 Cla97Chr01 12,183,702 ATCTAATAGAAACTCCCTTAATTTAAGAGAATAAAAAGCACTTTTCAAGCGCTTTTCACT[C/A]TGTAATTTTGATTTTTCCCAAAACAAGCTTAATAGCACAAGGCGATCATTAGCTTCTGTT 15
Cl-01-16745 Cla97Chr01 16,744,783 CAATTTTAATGGGTTAAAAAATTGATTATAACGTAAATTTAATTTGGTTTAAAATTTGGT[C/T]AGTTCTAATATTTGATTTAAAATTCAAATTTATCACTATTTTTTCTTTTATCATTTACGT 16
Cl-01-17502 Cla97Chr01 17,501,608 CACATTCAAGTTATGCAGAAGAAATAGTGGGAAAAGAGTGTGAGGTAGGTAGACGGTACC[A/G]AGTAAATCCAATTGATTGTTATAGGTTTCATGTGAAGGATGGTGGATTAGACGGTATCGT 17
Cl-01-23481 Cla97Chr01 23,481,140 GATTATCGCTTCAAGTCAACAAAGTCTCAACATTTATTTTTCTCAAATTTTTGCGTTCTA[C/T]CCTGGACTATCTGGGAACCTTATTCAAAATTTACACTTGGGTTTTGATAAGAAAATTTGA 18
Cl-01-24205 Cla97Chr01 24,205,357 GAAAAAGAATTACATTAACTATTGATAAACTATACCAAGTTTTAATAGTTAAGATAATAC[A/G]TTTCTTTAAAGTATAATTCGAATGGCAAATATTATTGATCTCTAGTTTATACTATACACG 19
Cl-01-25649 Cla97Chr01 25,649,173 AGACATTACAAACAGCCTAACCACAACAAGCCCTTGGATATTCCAAAGAAGGGGGAACAT[T/C]AGGCAACCGATCAACAAAAAGATAAAATGATGGCATGGTTTGCTTGAGCAACTAATCATA 20
Cl-01-26090 Cla97Chr01 26,089,918 AGTATTCGAGGGCAGTTCGAGCGGTTTTTCCAATTACAATTGTAATAGTTTAGTTGTTTT[A/G]AGCCATTAGATGCTAAAACCGGTCCACTTGAGTGTGTTTAATTAATTATGCATTTAGAAT 21
Cl-01-26834 Cla97Chr01 26,834,296 GTAACAATTTAGTCTTTATACTTTCAATTTTGTAATAATTTAGTCCTAAACTTTGTCGCC[T/C]TTATATTTTAAAATTTGTAATGATTTAGTCTCTATTATAGAAATTAGTGTTAAGGTTTAA 22
Cl-01-27352 Cla97Chr01 27,352,317 TTAAACTACATTTTATCAAATCGTAATAAAATTTTATTGATGGATTAATTGTAGTAAGTT[G/A]GGATTACAAATGTAATTGAATTGTTTTTGATCATGTTTACTTAGAATTACAAATTTGTTA 23
Cl-01-28251 Cla97Chr01 28,250,775 ATATGACAAAATTAAAGCATAAAACATTAACAAGTTTTTGCCAACAAAAATAATGTCCTG[A/G]ACAAATATTTGAATTTCAGAAAGGGAGTAATCTTAACCTTTAATGAGTTTGATGGATGAC 24
Cl-01-28784 Cla97Chr01 28,784,248 AAAGATGCAAAAACAATCCCAAAGCTAGCTTAATGCTCAGTTTAATTTTAAAATTTATGT[G/C]TAATGAATATAGATTTAATCAGAATTTATTTTACCAATAAATCAATGCAAAACTAAAATG 25
Cl-01-30753 Cla97Chr01 30,753,461 GCAGGTGGACCAAATAGTAAAGAGGCAAAAGATTAAGAACTTAATAAAATCACTAACCTT[C/T]TTAAGAAAGTGACTAGAGGATGGTTGTCTTTATGGTGAAACTATCCGATGCAATGAAGCA 26
Cl-01-31800 Cla97Chr01 31,799,743 TATCATAATGCACCTAGTTGTTTGAAGGTTCATTTTCTTGCCTTTGTTGCCCACCTAACT[G/A]TCTCTTGATTTCGGTCAATTGATCTTGAAAACTCAAAAGTTTTGACTTTAACATGACATT 27
Cl-01-32680 Cla97Chr01 32,680,368 AATTGTAAATAAAGTATTTAATAAGTCCCAAAGTTTCAATCTAAGATTAGTGAATAGGAT[T/C]TTCAACTTTTAATTTTGTGTCTAATAGGTATTCATACCCTTAAAAAAAATAGATCTATTC 28
Cl-01-33522 Cla97Chr01 33,522,023 CTAAACAAATAAATAGGGGAAATAGAAAAAGACTAGAAGATAAGAAAAAAGGAAAACCCA[T/C]CCATTATTGATGAAAGCCGCTACCACAAAATTTGATTTCATTATTTTCCGGAATATGTTG 29
Cl-01-33955 Cla97Chr01 33,954,998 TATTATTTATATTACATTTATATTAGTGATATTTTGTTGCTTATTTTTTATGTTTCTTAA[A/G]TTTTTCTACGATATAATAAAAATATCGATTCAACTCTCAAATACACATGAAAATATCTTT 30
Cl-01-34164 Cla97Chr01 34,164,151 AATTAGGCGTCGAGTTTGTAAACAATTTGATTTCACCGCATACTTTGCCAATCATGCATG[T/C]GTCCAACGCATAATCGAATTATGCGTTCATCGATAAACGATTTCTGTGTTTATCAAGTTG 31
Cl-01-34362 Cla97Chr01 34,362,097 AATTCTTATGATATCAAGCTATTTATTTAAAAGAGCAAAAGTTTAGTAGAAATAAATTCT[T/C]ATGATACCAAGCTATTTATTTAAAAAAGCAAATTTCACACGACATGTTTGGTACTGATTT 32
Cl-01-34775 Cla97Chr01 34,774,625 TGAAAGTATACCGATGTTAATATACATACATATCTCTTCTATTATATTATGATGTATCAT[A/G]TTTATAGTATCATACTTCAAATGAACATCAACTTCGTTATGTAACTACCTAACTCCATTA 33
Cl-01-36667 Cla97Chr01 36,666,756 TCAAAGGGAATGCAAAAGACGTGTCCAATTTGCAACAAGAAAATCTGAACTGTGGTCTTC[G/A]AGAGGTTGGTAATCTTTACACCAACAAAAAAGCAAGAAAAATAGAAAAATCAAGGATATC 34
Cl-02-444 Cla97Chr02 444,411 TGAATTAATAGAAATTATTAAAAGATCTATGGACAATGTAAATATTCTCTCTCATTAATT[A/T]GTAGAATTGATTTAATGCATGCACCAGCATCTAAGAACTTGCTTAAGAGTGGAGAAAAGG 35
Cl-02-1020 Cla97Chr02 1,020,439 CACCCATTATAGGAAGACATACTAGTGGCGAATTTCTCTTCATTTCTTTGTTGTCCATGA[C/A]CAAGACGGCAGTTGATTAACTGAGCTGGAAAAGAGTCAAAAATATTAAAAAAGAAGAGAA 36
Cl-02-1164 Cla97Chr02 1,164,470 GTAGAAATTTTGATTTTTTTTAGTACTGACAATTTTTTCTACTCTAAATTTTTTGTGGTC[T/C]ACTAAGTACACAGTAATGAAAATTATTTTAAATGACAAAAAATTTTTAAAAATATTTACG 37
Cl-02-1703 Cla97Chr02 1,702,723 GGACAAAAGCGCAAAGAAAACAACCCTTTTTTAAAACAACAAATATACATGCAATCCATT[C/T]AAACTCAACTAACATATAGATATGTCAGGGATCAAGAAGTTTATGGTTCTAATCTCTCTA 38
Cl-02-2476 Cla97Chr02 2,475,619 GGATTATTTATGTAACCAATTTTTCCATCTTTTTCAAATTTTTTCAACTTTTTTTTTTTT[T/A]TAAATAAATAAATAAATTTTTGGGTACAGATCAAGGCATCAAAGAGATGAATGCAGGGTT 39
Cl-02-3291 Cla97Chr02 3,290,718 AAACAAAATATTTCATTGAGTAAATGAAAAGAGACTAAAGCTAGAGATACAAATTCCGTA[A/C]GGAAATGAAAAGAAAAAGAACAAAAAATAATTCAGTCCACAAATCTCAAAAATTAAGAAG 40
Cl-02-4734 Cla97Chr02 4,734,030 ATCCAACACAAAATTAAGAAATAAATGATCCAATTTGACACATCACAGCAGTATATATCT[C/T]CCACTAATAGATATGAAGTTTAAATTCATAATAGTAAATGTTGAATGAACTTAAAAAATT 41
Cl-02-5653 Cla97Chr02 5,653,411 CATATATATCATAAGAATGAGAGACCAAAGGTTTACTTTTTCTTCCAAATGAATGCAACA[A/T]TTTCAACCATTATTTTTCATGCAAGAATTAGAAAGTAAAGGGTCAGAGACTCAGACTGTA 42
Cl-02-6125 Cla97Chr02 6,125,359 CATGCTCAAATAGCATTATCAGACCATATACATTTCATTTCCGAAAAACCATGAAAAATA[T/C]GATACATTTCAAAACATTTTCCTTAGTTGAGGCGGAGCGGAGCTCAACGCTACTCAACGT 43
Cl-02-6626 Cla97Chr02 6,626,380 TGAAAATTGATACATCCTTTAAAACAAAAAATGAATTAAATATAAAGTAAAAATAAAGGT[G/T]ACATTAGAAGATAACAATATTATGATCAATTCCCTATAAACTAGACCTACATAATTACAA 44
Cl-02-6886 Cla97Chr02 6,886,201 GTTTACTACTTTATATAGACTTTGTTGTTGATTAATATATTTATTTTCAAGTATGCCACA[T/C]ATATTCATGCGAATCTTAAAGTTTTAAATGTAATTTATGTTGATGTAAATAAATTTATTT 45
Cl-02-12006 Cla97Chr02 12,005,694 CTCAATGGCCCTAGAGGGGTTAGTTTATAATTGGACTATAAATAGTTTGTTCATTAGAGG[T/G]TTCAGTGGTACTTAGGAGTTAAATATAACTACAGGGGTAAAACGGTGATTTGACCCAGCT 46
Cl-02-12257 Cla97Chr02 12,257,073 ACAAAAGAAGACTATTAAACACAATGAACTAACAAGTTCAATATTTTATTTACACCTTCA[T/A]TGCCCGAGATTTTTGTTTTTATTTTTTTGATATATGATTAGCTAAGTTGGTTGTTGGAGT 47
Cl-02-12976 Cla97Chr02 12,976,434 TTAATGGGAACATAATCACACCATAGTTTGCTACAAATTTGGATAATAACCAATTAATCC[A/G]AGGAAGCCTAGCATTTGCTTAATTGTTGTCAGTATTGACCCATTATTCATTGCCTCCAAC 48
Cl-02-13438 Cla97Chr02 13,438,428 TCAAATGTGGGATGTAAGATTCCTGGCAAGAAATCATATAAATTTGGTGACGCATATTTT[T/C]GCATTGGCCTATTTCTATCATGTGCTAACAGAATAGGATTAGTTTGAGGGTGTTTGTTTG 49
Cl-02-17728 Cla97Chr02 17,727,675 TCAAACTAAACTTGATTTCATTATGTACAACGATAACGCCTTCAACAACGATCCAAATTC[G/A]CATTAAGCATAGCAGGGTTTAATTGTGACGAATCAAAGGTCAGAAAGCCTTGTCTAACAT 50
Cl-02-21833 Cla97Chr02 21,832,867 TTCTGATTGTCAATTCTGATTCATGCATAGGAAATGCAACTCTTCTACTCACAAGCTTGC[G/A]TGTACTTGTAGAGATTACTCTAGTGTTACCTTTTTCATGGATGCCTAATATTTTTAATTA 51
Cl-02-25756 Cla97Chr02 25,755,545 TGTATTAGAAGTTGATTATACAAATACTATGTTAATTGAGCTATGTTTGTTAAAAGAAAA[G/T]TTGAAAAATGAGATTAAAAATTGTAGGAGTATATTAAATAGTGTAGTGGAATAGGAAAGT 52
Cl-02-26158 Cla97Chr02 26,158,379 ATTGTTTTTTGGGAATTTGCAACAGGTGGCAAATCCAAAATGTATAATTGGGGGCGTGCT[A/T]TCAACGTATGGAAAATTGTTGAAATGACAAAATTCTCAAATTTAAATTTTTGTTTATTCC 53
Cl-02-27086 Cla97Chr02 27,085,635 GTGAGACATTTTGAACAATCAAACTACTAATCAACAATGGTCGGAAGTAAATGACAGCCG[G/A]CCTTAATTTTAGAATTTTTATTGTGGTTTTTATAGATTTAGCTTTGTATATATAAGTCGC 54
Cl-02-27600 Cla97Chr02 27,600,340 CCGAAAAAACACCGCTAAAAAAGTATTTACAACACGTAAAGGCACGTCGCTGTATACGTC[G/A]TAGTAGCTGCATAGTCATAAGTTTTAGTGACAAATTCCTAGCACGCTGCTAAAACTTAAA 55
Cl-02-28183 Cla97Chr02 28,182,891 GGGCGTTGGACTTCTCCAGTAGCCAGTGATCGTTTGTTACATTAGTGGCTAATGTGAACG[G/A]TATCTACCTTAACATGTTTTTCTGCTGAATTCAGATGCTTATAGAAAATGTTTGACTGTG 56
Cl-02-28417 Cla97Chr02 28,417,374 AAAAATAGAAGGCAATTATAATAGGTATGAGTTCATTTTTCAATTGGGTATAAGAAAATT[A/G]AGGACCAATTATGTGATTTAGCTAATTATAATAACCAATGAAACTAAGTCCAAATTATAT 57
Cl-02-29689 Cla97Chr02 29,688,874 AGACTATGATCTCACCATTGTGGTATATGATCAATTTTAAAGTATGATTGTCCAATATAT[T/C]AGTGTTTACATTCATTTTCTTCGGGTAAATATTTTGTCAATAACAAGGTAGATTGATTGT 58
Cl-02-30234 Cla97Chr02 30,233,854 TCGTATTTTACTTACATTTGTTGTACTAAAAACAAAAACAAGAACATTTCCTTTATTTTA[T/G]TTTATCTTCGATTTGATCCGATTATTGTTTAAAAAGATTCAAGTTGGTTATTATGTTTTA 59
Cl-02-32443 Cla97Chr02 32,443,231 ATGGTTTGTAGTATAAGTATAACGTTGGGTGCCTTATATCCTGGTGACACTATGAATATG[G/A]TCCACTTTGTAATTGTTACAAAAGTTGTAAAGTGTTACAAACGATTTGATCCTAATCATT 60
Cl-02-32956 Cla97Chr02 32,955,658 TGATTTGACTTAGATAAATATTAGTGATAATCACTTATAAACCCCTTATTAATAGACTTC[C/T]ATCACTTATAGCTTTAAAATGTTAGTCTTTGAAAGTTGATATTTTACTTTCACATTAGTC 61
Cl-02-33561 Cla97Chr02 33,560,636 TATTAGGGGTGAGTATTGATAGACCGAAAATCGAGCCGACCGACCAAAATCAACTAAATC[T/G]AAGTCAATCGGTCGAAGAAGAGGGGGGACTTAGTTGGTGTCAGTTTGGAAAAATTTCAAA 62
Cl-02-34490 Cla97Chr02 34,490,489 TGATAATCCGTGTCTAAACCATTCTTTCAAAGTATTAAATCCTATTGATGTATTTCATTA[A/G]TAACATCCCTAACATAGTTTAGTGAATAAATCACAAACTAACACTTCAAATTTAGATTTA 63
Cl-02-35524 Cla97Chr02 35,523,550 TTATTTCAAGTTGTTATCTTGGCGTATGTAACTTTTGTATTGTGTTGGCTTAGGGCAATT[C/T]TACTTTTTAATTATTGTTCTTTTATCTAAAAAAGAAGTGATTTGACATTCATATGAGAGG 64
Cl-02-36771 Cla97Chr02 36,771,218 AATTAGGTTTATAGTCCTGATTTATTATTTTTACAAATGGCAGAAAAACAAGTTCAGCCC[A/G]CGTGATACACCTGATATACATGACACACAATACACAGTTGATACACACTTTGATATACTT 65
Cl-02-37150 Cla97Chr02 37,149,765 GAAAATTTCTCTAAGAGGAAGCAAAGTGCCAAATGGGCAAATGATTGCTTTGTGCTTGTC[A/G]CTGCCAATCCCTCATTCCATTTGTTCTTTAGGAACATTTTTAACTAACAAAGACGAATCA 66
Cl-02-37324 Cla97Chr02 37,324,405 TACTTTAAATTAACTTTATACACAAACGATAAATATTCCAATTTTGCGTTTCCCTCAATA[T/G]ATTATTATTTTTTAAATTTTAATATTACATCTTACTCTCTTCATAACCATTTCGCATTTA 67
Cl-03-64 Cla97Chr03 64,354 TGTTACATTCTCAATAATTTAATTAAAAACTTATGACAAAAAGTTACAACTACAAAAATT[A/G]TAAATAATTATACCCTACATCCACTTAATATCACATTTCCCACCAAGTTAAATAATCTTG 68
Cl-03-85 Cla97Chr03 84,706 TAAGTCTTAACTTTCAATTTTGTGTTTGATAGGACAATATTTTCTCAATTGATTTGTGAA[A/C]TTCCAAATTTATAACAAATTGTTTTTCACATATTTAAATTTTTAAAAATTAAGTATTTTT 69
Cl-03-267 Cla97Chr03 267,360 AATATATATATATACATCAAAGCCAAATAAAAAAAGTGATAGATGTCTATCTGGATCTAT[C/T]GCGGTCTATCACAGATAGAAAGTAAAATTTCGTTATATTTGTAATTATTTTCAACAGTTT 70
Cl-03-2166 Cla97Chr03 2,165,689 AAAGAAAAATGAAAAAGTTCATTGTGTATAAACAAAGTAACATTTTGCTCATTCTCATTG[T/C]ACAGTTGACTTTTATTGAGTTCATTTTTCATTAGTAGTTTAGATTGATCACCCATATTAG 71
Cl-03-4129 Cla97Chr03 4,128,659 ATAGTGGTGAAGATATTTTGTGTTGGATCAATGGTGTGGGTTTTGTTGGAAAATAAAAAA[T/G]AAGTAAAGGGAAATGTAAGGGATGGGAAGAAGCTAAGGCAATTATTGAGAAATGAGTTGG 72
Cl-03-5719 Cla97Chr03 5,718,677 TATGTTAGTCAAATCAATGAAAAATATATGATTCTCATCAATTTGTTCCCCACTTCGATC[C/T]AAATAGGTGTAATAAAAGATAAAATTAAAATTATTAAAAGATGCGAGGAAAAAACTGGAG 73
Cl-03-7170 Cla97Chr03 7,169,903 TTAGTGTCTTCTCATAACCAAAAGGGTTGCCAATGGCATAGCAGCTCTGACCAACACGTA[G/C]ATTTCGAGAGGCACCAAAGACTATGGGCTTCAGTTCTTGTCCTTCAAGTTCTACCTGGAT 74
Cl-03-7398 Cla97Chr03 7,398,031 AAGGAGATGAAAAATTAAGGTTGTATCAAGAGTATATTAATGTTCATTATTGTTGAAGGG[A/C]AAATTACAAATAACAGAAGAAAATAGAGTAAATATTGAGAAACAAACACATAAGCAATTA 75
Cl-03-7503 Cla97Chr03 7,503,062 TCTTTTATACTTACATAAATTATATATTGAAATGGACGCTCGTCATATTTAAGATCAAGT[G/A]TTTTGGCATCGATTTGCTACTCGTCATTTTTACTAAGAGATTCTATTGTCTAATTGTCTA 76
Cl-03-7533 Cla97Chr03 7,532,741 GGTAAAATATGACAATAGAATCAGAATTATTCAAATTTAATACTTTGTATTAATTGGTTC[A/C]TATTTCCAATTTAACATATGTGAAGAAACCAATTAAAGGACTGTAAAGCGGGAAGGAGTT 77
Cl-03-8839 Cla97Chr03 8,839,080 ATCTATAGCTTTCATTCATAATTTGACATGTCATCAAATGATATATTAATTTATCATTCA[C/T]TTATTTTTTGGATCCCACGTAGTTCTTGAATACTACTTTATTATGTGACAAACAATGAAT 78
Cl-03-10182 Cla97Chr03 10,181,930 TGAAGGCCTTAGAAAAGCAAATATATGTTTGCGTAGAGAAACAAAATTTAAAATTGATAA[C/T]ACTTTGTTCTTTAGTAAGTCAAAGAGAAATTTAATAAGTTTTAAAGATATATGTTGATAT 79
Cl-03-10667 Cla97Chr03 10,667,331 TAATAATACTGCTAATGAACTTTATTGATATACCATTGATATACTTTATAATGATATACT[C/A]ATTTATACTGTTAATACACTTAACTAACATATTGTAAATATACTTTATAATGATACACAT 80
Cl-03-11205 Cla97Chr03 11,204,515 TCGTTCTTTGGATCCCCGGCAATGGCGCCAAAAATTTGATGCGCACTTTCCGTGTTTACC[A/G]CAAGTATACGAGTCAAGTTATAATATAAGACGATTAAGTTCCGAATATCGTTCCACTGAG 81
Cl-03-11317 Cla97Chr03 11,316,573 TCACTCCATTAAAGACCTACAACTGCACTATTCACACTATAGATATATTTCTATGTCCAT[T/A]GAAATAATCTATCAATAGTAAGTTGACCCTTCACAAATTACTCGTAACTACAATTGGGTC 82
Cl-03-14143 Cla97Chr03 14,143,161 GAGTGTTACCTATTCTACAATCTGTTAACACTGTTATATATCATAAACTGCTGGCAATTG[G/A]CTTAGCTTTTACAATAGTTATGCGATAATTTACTATAGTTATGCAATAATTTACTGATAG 83
Cl-03-14839 Cla97Chr03 14,839,461 AGTAGTATAGCTCAAAAGTTCTTACCAGATCTGAAAGTTGTTCCAATTATGCATCTTCCT[G/A]TCACTCTTTATTGTGATAATAGTGGTACAGTTGCAAATTCCAAAGAACCTAGAAGCCATA 84
Cl-03-20863 Cla97Chr03 20,862,899 CTAGACTTATACAAACTCATTATATAAGATATTGATCTCACTCACATGTCTCTACATGAA[C/T]GATTTGGATATAATCATTTGCAACAGTTACAAAGTGAGTCGTATCAATAATGTTATTAAG 85
Cl-03-21132 Cla97Chr03 21,132,223 CCGAAGTTTTTCCATTTCCTCGAAGCTTCAATCCAATCTTCTATCTGCCTTGTATCACAT[T/C]TAATCAGTGCTTTGTCTACGAATAGTGGGTTAATTATGAATTTTACATTGAATAAATCTT 86
Cl-03-21472 Cla97Chr03 21,472,119 TCGACACTGTAGACAAAAGTAGCAAAACACAGTAGCATCACAACATTGTCCTATAGCACT[G/A]CACCGCTATGACTTTTCCAGCACGCATCCCACACATTTTGTTTTTGGACAACGGTCCGAT 87
Cl-03-23928 Cla97Chr03 23,928,431 ATCTTTCTTTTTTGTTCTCTCTATGCATTATTTTCCAAGTCTGTTCAATCTTCAAAGCAT[C/T]TCAAGATGTTGGTAAATTCAAGTTGGAAATGATTCACCTCAACTTTGAAGGATTTGAATA 88
Cl-03-24321 Cla97Chr03 24,321,207 TAGGTGTTTGATGAAAGCCTTTAGCAACAAGTCTTGCTTTATAACGAGCAATAGAACCAT[T/C]TGAATTCCTTTTAATTTTAAACACCCATTTACAACCAACAATTTTCTGATCAGTAGTTTT 89
Cl-03-25448 Cla97Chr03 25,447,696 AATCTTATATTTGATGGGATTAATGGTCGAAAACAACAAAATAAGTTTGAGATAAATTAC[T/G]AACGAAATTTGAAGGAAGCGGAAGGATTAAAGTCGTAAAATTGTTGACTCTCAAGACATA 90
Cl-03-25513 Cla97Chr03 25,513,425 TGGACCAATGAAATCTAGGTAACCAATAAAAGAAGTTTATATGGAGATAGACACGAAAGT[A/T]AATTTAAAACCATAAACAATAATTGATAAACAAGGGCAAACAATAATTCAACCTTCGAGT 91
Cl-03-25563 Cla97Chr03 25,563,003 TTTAATGTTATTTCTTGGTTGGGATTGTTCTGATTTAGATTTAGTTATGTGATAAAAAAA[A/T]TTTTTGTTTCAACATTTTATAAATCCCCAAGTTTTCATTCTTAGAGTAGGTAAAGCCATT 92
Cl-03-26664 Cla97Chr03 26,664,066 ATTCGGATTAGGTTTATCGTTGGATGAATTGCATTGCTGATATTGACCCTAAAAAAGAAA[C/T]GGTAGGTACAGCTGGTTCGTGAACAACCAACCATCGCACTGTAAAAATAGGATAAGTTTT 93
Cl-03-26913 Cla97Chr03 26,912,769 CCATTTAATTAGATGAACACAACACAACCAATTGGCTAATAACATTAAGAATGAGGAATA[A/T]GCTTAGTTGAATGCCCAACTGGAAACGTCAAATCACTATTCAATATAATTCTTCCTACGT 94
Cl-03-27651 Cla97Chr03 27,651,358 TTTCGTGAATACCCATTGGCGTTGGTTTTAATTGTTGGACCCATTGTTTAAAGTATGGAA[A/G]ATGACTGTGACAGTTGCACTTTGGATCCGTTTTCGAAGGGGCTTTGGAGAAGGTTGAGAT 95
Cl-03-28017 Cla97Chr03 28,017,048 TTTCTTTCATTTCAAAGACCATGGACATCATTTGAAAATAATTGAAAGTTACTTCAAAGT[G/C]AGGAGATATCTTGGTTAATTAAACCAAAATTATTTGATAATCCATCTAAAATTAGGCACC 96
Cl-03-29643 Cla97Chr03 29,642,943 AACTTTTAACAAAATTGCAAGGTGACTTGTAGTTTTTGGATGACTAGACTACTAAATAAG[T/G]TAGCTATGCAAAAAAATCTAAAGATTCAATTTTGAATAAGATGGCAAAGCTGGAATCTAA 97
Cl-03-29796 Cla97Chr03 29,796,436 TTTATGGAGTTAACTTATAATTTAAGGGCTCTTTGGCCCATATTTGGGGCTCCAAATGTA[A/G]TAATAATATTATAACTATTTATAATCTACCATTATTTGCATAGTAATATTTGAAATCTTA 98
Cl-03-30122 Cla97Chr03 30,121,605 TGGTTAAGCCATGATGATTGGTTTACAATAATGCCAATTAACTAATCGATATCTCTTTAT[C/T]CCTAAGCCACTTATGACAACTATGTGACATTTTCGGTAATGTCGTGAAAAGTCACGTAGC 99
Cl-03-30277 Cla97Chr03 30,276,890 AGAAATTATTTTAGAGTGGTAGCATGAAGGAAAGGCTAAAGTTCTATTGCGTAGCACTTC[C/T]AGAGCTATTTTGTGGATGATTTGGATCATTGACTGATAGAAAACTTAGAGAAAAGATTCA 100
Cl-03-30353 Cla97Chr03 30,353,102 TATTAGTAATATTTATTTCAATTTTAGTTTTAAAAAATAAAAATTTTCACACATAAAACG[C/T]CTTTTTATTTAATTTTTATTTACTTAATAAGGATTCGCAACTGAACTTCAATATATATTA 101
Cl-04-639 Cla97Chr04 638,604 ATTAAATAGGGGAAGTTGCAAACATATCAATCAAACTCAAAATATTAGTAGATATAACAC[A/G]ATGAAAAAATATTTGTAGTTATGGAAAAATTTAAATCTAACTTTCAGAGTCTATCCATGA 102
Cl-04-1463 Cla97Chr04 1,463,007 TTATCACCTGTCAAGCCACTTGCTAGTCATTTCTTCCAAGAATGTTAAGAATAAAAGTAG[A/G]GCTTACAGCGACACTAGCCTCATTTTGGAAAAAAAATCTACACTTTTGGAAAGTTTTTCC 103
Cl-04-2866 Cla97Chr04 2,866,424 AACACAAGAATTAATGTGGAAAAACTCCTAACTGGAGAAAAAACCACAAATTAGAAAAAT[T/A]CCGGTGTGTGAAAAAATGTTACAATCACCCAAAATAATTTCCTCCATGGTTCTAATTACA 104
Cl-04-4455 Cla97Chr04 4,455,181 ACGAGGAGGAGTTAAGCCACGAAAATTTAAGGTAAGCTAGAAAACATATTTCTCTACAAG[G/T]TTGTAGAAGGAAAAAAGTTCCAAATTCGGCTGGAAATATGACTTTTAACAGATAGAATTG 105
Cl-04-5168 Cla97Chr04 5,167,752 GAATGCATACTAATAGGCCTTAGGTTAATTCAACTTAGGTTCTAATTCAGTTCCCAGTTA[A/G]GAGGTGTTCCGTAAACCGTCAACTTAAATACCACAATCGTGACAAAACTGAAGTGGTAGA 106
Cl-04-5673 Cla97Chr04 5,672,993 CTTTGGGTGAAGTTATTTGAATTAAGTTTTAAATAATTTGTGGTTAGATTTAGGCCTAGT[G/A]ATTTAATTTAAGTTTTGGTTTATGTTCTAGGTTGTAATTCAAAGGTTTTGGTGGAGTTTG 107
Cl-04-7179 Cla97Chr04 7,179,260 ATGTATTTCATATCTAATAATTAAAACTATTCTAATATTGTCACCCCCACCTTAGATTAT[T/C]CTTTTGAACCTAGGACGAAATTTTGACGATAGTGGAATCTACTATCAAAACTTTTCTTAT 108
Cl-04-7249 Cla97Chr04 7,249,330 TTACAGGGGTAAAACGAACATTTGACCCAGCTGTAATTATGAACAACTCGTGTAGGATTG[A/G]CTTCCTTATATTGATTATATCCTTGAATACAACTTGTTATATAGTGCACAAGAGTTCAAT 109
Cl-04-8581 Cla97Chr04 8,581,261 AGTTATGGACTCCTGTCTACATTCCTTTGATTTGTATGGGTGAGAGTCGCTCATATCGTC[A/G]ACTCAATAAGCCTACCATCTTGCGAACTTATTCAAGTTGGGAGCTTGAAACTTAGCTACA 110
Cl-04-11192 Cla97Chr04 11,191,944 CTCCTCCTAACACACTCTGCTATGTGTTTACCATACACCTTATGCATCCAAGCTTTTGAC[T/G]TCTCCCAAGATGTGTGTTTTAAGCTTGGTTCTAGTCCATTAATCTAACTCCCAACTATGC 111
Cl-04-11837 Cla97Chr04 11,836,594 TTGATATTCTTTGCCAGGACTCTTGCAAGAGGTTTAGTATGATATAAGAGGTTTAGTATC[G/A]TCCTTAGGGATCAAATATTATGGATTAATTTATCTACTTTAGAATTATGTCAATTTTATT 112
Cl-04-12341 Cla97Chr04 12,341,422 CAGTATACGTTTTTCATGTGAGACAGTAGATATAAAGATACTTTATATTATTCTTACCAT[T/C]AGTCTCAATATGATAACCATTGCAACTTATATATTTAAAACTCATTAAATTTCTCTTTGA 113
Cl-04-13817 Cla97Chr04 13,816,937 AAATGAGGGTAAAACCTATTGATTGAAGATGGATCTATAAAAGAAAACAAGACCAAGCCG[G/A]TAAGGTACAGATCTTTAAGGCTCAACTAGTGGCGAAGGGTTTTATTCAGAGAGAGCGGGT 114
Cl-04-14131 Cla97Chr04 14,130,979 ATAGAGATTGACCCATAGGGTCAGCCATAAAGCCATAAGTTTATGGGTTGGTTGCACAAA[T/C]AACAATCAGGCTCAAACTAATAAAAGATGTAGCACATGAAAAAATATATTAGGGATATAA 115
Cl-04-15133 Cla97Chr04 15,132,726 TGGAGGATGCAATTATATTAAGATATTTAATTGGTCTTATATGTGTATGAAAATCTGGAC[A/G]TGCATCTTATGGATGTAGTCACAAGATATTTATATGGACTCTTAATAATGACATTTATAT 116
Cl-04-15298 Cla97Chr04 15,297,971 CACGAGCCCGAGTCGAGGTTAAGGTCCAACTAGAACTTCAAGAGATGAGGAGATGATTGA[A/G]AAAAATCTATGAATAATCCTAATAAAAGAAAGTATAAGCAAGCTTTGGTTGAGACCAATA 117
Cl-04-16244 Cla97Chr04 16,244,465 AGGCCTAACAAAAGAAAATTGGACTAAAGAACTATTAGTTGCTAGTTTGTTGGGTATTCT[G/A]ATTGCTCTCAAGGTTTCAAGTTTATGATCCCACTTCTAAGGATGAGTTTGTGGGGGAAGA 118
Cl-04-19602 Cla97Chr04 19,602,040 CCTTTGATTTGTATGGGTGAGATTAGTCGTTTTTCAACTCAATATGCGTAGCATCTTGGG[G/A]ACAAGATCAAGTGAAGAGCTAGGGACATAATTATATAAGATGGAATTCACTCATTCCCAT 119
Cl-04-19756 Cla97Chr04 19,755,762 GAATATTTAGTTTACTTAACATATAATAATGTACGAATATTTTTTTCAAATTTTATGGAT[G/A]TATAGATATATGAAGCAATTTTATTTTCATCATAGAAATTAAATCATTTATAACTATCTT 120
Cl-04-22344 Cla97Chr04 22,344,024 AACAAATGTCAGAGTGTCAAATGTCCAACACCTATCGGACACAGACATGCTAGCCAATCT[A/G]AAGTATTTGTGCTTCTTAGCTCAACTGAGTGCAATTTGTAGATTCTACCAAGCCTTTTTC 121
Cl-04-22605 Cla97Chr04 22,604,704 AAAAGAAGGAGAGAAATGTCAAACCCATTAGATGCAAACAATATTGATTTTTTGTAGGCG[G/A]AGCTAGATCAACAACTCTGTCGCGAGACATGAAGATCCTATGTTGGAATGCCCATGGATT 122
Cl-04-23088 Cla97Chr04 23,087,986 TCAATGAATTAAACCTTGAATTGACAAAGAGTGTTGTTCTTTAGGAATCAAAACCATGTA[G/A]AGATTGAGTAAGTTCCTTGAGAGAAGAATTATCTATCACAAATATATATACATAGGCATG 123
Cl-04-23092 Cla97Chr04 23,091,872 TTAAAAAAAGGGAAAAATATGATCAATTTATATCCACAAACTTTAAGGTTGTATTAACTA[A/G]TTAAAATTCTAAACTAATAGTTGTACCAATTTAAAATTTGAAATTTCATAACTACATCAA 124
Cl-04-25311 Cla97Chr04 25,311,332 TAATCACAACTAAGTTGCTTTCTTTCCTGTACTTGTGAATTGAATCCCATAGAAAACACA[G/A]ATTTCTATTTCATAATTATATTCTATATTACTTTGACATGTTCATTCTACAAAAGTTCAG 125
Cl-05-358 Cla97Chr05 357,701 GTACTGATATTGTCACTCTTAAGAGGAGCCTAAGTACTCTAAGAGGGAGTCTCACATCTA[T/G]GAAGAGCTTAGGTGGTTAGCTTGTTAAGCTCTACAAGAGTTGATGTAGAAGTTGGTGTTT 126
Cl-05-686 Cla97Chr05 686,175 AAGTTATTGTTTATAGTGTTTGTATTATCAAGTTTGAGGTTAAAGGTTTAATTTTTTATC[C/T]ACATGTCGTAAAAGAAAAACAGGGTACATGTCGAATTCCCTAGATGAAATGCACAATTAA 127
Cl-05-2679 Cla97Chr05 2,678,825 ATTTATAGTTATTGTTATGTAGAGGTCAAATTTTTTTCCCTATTCTAATTAAGGCATCAA[T/C]ATGACATGAATTTTTTTAGTTGCCATAAACATACACTTAAAATCAACATACTAAACAAAG 128
Cl-05-4203 Cla97Chr05 4,203,163 GGATTCCATGTGGAAAATCCCCTTCCAGGTACTCGTCGCTAGCCATGATATTATCTTCAT[C/T]TTCAACTTCACTACTCTGACCATATGCCTCGGTACATATACCGTAATCTTCTTCCTACGA 129
Cl-05-5319 Cla97Chr05 5,319,371 GAGTAAGAGACATGCATCTCAACTTAATTGTTTGCGTGTTTTCTTTTTCAATATTTACTT[C/T]AGTTTCCTTTATTATTTGTAATTCCCTCGGCTATATATATTAATTAGGGGTGTTATATTT 130
Cl-05-6999 Cla97Chr05 6,998,762 ATCAACTATTTATTTATTATTTTGGTTTAATTACTAGTTCGGGTTTATTTGACCTTTGAA[G/A]TTAGAAAAGAAAAATAGCTAAATAGTCTTTCAACTTTCAATTTTGTGTTTAATTAGTTCT 131
Cl-05-7154 Cla97Chr05 7,154,378 TGAAGATAAAACGTTCTCGTTAATCCTGAATAGCCTCATTAACAATGAGAACTAACTCCT[C/T]GTCATAAATGCCCCTGGGCATCTAATATTGTCGAAGGGCGAACTTTTTTCAATCTATTAC 132
Cl-05-8322 Cla97Chr05 8,322,073 CCAAAAAATTAAGATCCAATCCAAAAATACACCATAAACTAACCTATGTAAAGTTACTAA[A/G]TTTGACCCATTAATGAAACTAAACTTAATTACATTGTTTAATGTACATTTTTATGCATAT 133
Cl-05-8849 Cla97Chr05 8,848,691 ACTATAGACCTTGTGGTTAGTACGATCATATGTCAGTTGAGCTATACTCGTTTTAACCTA[C/T]AATGTACTTTCTTGCATGATATTATCATTATTTTTAAAACCAATTGAGATCAAAATTAGT 134
Cl-05-10676 Cla97Chr05 10,676,245 GGAATATTATTTGGATTAAACTTATGATTAAGTTGTGATGCATATTTCTGAATGTATATT[G/A]GAATGACATTAATTGTTATCCTATCTATAGGGTCATATACTCTCTATTGATTGTGAACCT 135
Cl-05-10974 Cla97Chr05 10,973,959 TCCCAAATTTAAGAGGATAAAAAGTCTTTTTTGAGTGCTTTTCAAATCTCGTTTGGAAGC[A/G]TGTGATTGCTTTACTTCAATTACGTTCAACAATTAAAACACAAAATTATAACATACAATA 136
Cl-05-11366 Cla97Chr05 11,366,326 ATAATAATAAATAATGATTTCTTATGCGTTGGTAAGTTCTCTTCCATCTTGCCCTCAAAT[T/A]AAAAAAAGCTTCTCTCCATTGATGATAACATAGCTTAAAGTTAAGCCCTAATTGATGAAC 137
Cl-05-11786 Cla97Chr05 11,785,817 TATCCCTATACTATAGACCTTTTAGACTGTATGTTGAACATTGATCTCTGTATATCCCCA[T/C]ATACGGTTCAAAACTCATAAAACAGCTTAGGATGTTAGTTTATTGGATTTAGGGTTATTA 138
Cl-05-12299 Cla97Chr05 12,298,751 TTTGTCTACGAAAAGTACCCGAGTGGATGGTTAGGGATCCTGCCTGGAAGGTAAGACTAT[G/A]TGCACATAGGGGAAACATAGGGAGACTACAGTGAACATTTATACACATATTTATAAGTTT 139
Cl-05-12891 Cla97Chr05 12,891,141 ATAGAAAAGAAGTCCTGCAGAAAGGTAAGATAAACATTAATCATGAAACTTGATTTTTTT[T/A]ATTTTTTACAGTCACAACCACTTCCATGGAACTTGATAAATCAGTACTTCATTTGATGTA 140
Cl-05-13598 Cla97Chr05 13,598,023 TTGGCGTAGCACAAAGCAAGGTTGCATTGTTGACTCTACCGTGGAGGTAGTATTGAAAAG[C/A]ACCTAAATAATGCTTTGTTTGCCTTTGATTTAGGGATGTTTTTATTAGATATGATGTAAT 141
Cl-05-15155 Cla97Chr05 15,155,281 ACAATTACAAATGGGTCATATCCGAAGTGTTACAAGAATAAAGCACCCAACCTTATTCCT[G/T]TTAGGTTTTATGCCCTAAAACTCATAGATTGTAAATGTAATAATTTGACCGTAATTAAAT 142
Cl-05-16805 Cla97Chr05 16,804,998 CATACACAAGCCCTTAGATACTTGAGGATATTCTTAACGGCAATCCAATGTGCAAACCCG[G/A]GATTGGATTGATACCTATTGACAATTCTTACTGCAAAACATATGTTTGGTTGTGTACATA 143
Cl-05-17453 Cla97Chr05 17,452,758 ACTTGGTTTGTTTGGTCTGTCTCTATTTGAACTTTGGATCCTCTTCTAATCTTGTAATCG[G/A]AGTTAATTTCATTGATGTTTTCATGGTATATGATTCTAAAAACTGATCCAACTAAATCGA 144
Cl-05-18687 Cla97Chr05 18,686,904 TTTCGACCATATTTTATCGAAGATCATTTAAACCTTTGAAAAAAGTATACATTTATCAGA[C/G]GTTTTTAGAAAGCCTTCAAAAAATACATCCATTTTATAAAAGATTATAAAAAACCTTTAA 145
Cl-05-20440 Cla97Chr05 20,439,854 CTTTATACACTTCACTAGCTATCAACACATCACCAACAGGAGTGTAAACAGACAACTCCT[T/C]AGGTAATGGTTCTATTGTACTATTTACTCTTTCAGCAAACACTTTAGCAATAAAAGAATG 146
Cl-05-21316 Cla97Chr05 21,316,300 ATCTCAAGTTTTTTTTTCACAATTTTAGCTAACAAAATATCTTTTACCTTACCAAAACTT[G/T]TGAAGTTAAACCACACTTGAAGACTGAAGTTCTTTGGAATACATACATTCTTCCAAGGCT 147
Cl-05-21892 Cla97Chr05 21,891,975 GACAAACAAACATATAATCCGTGGAAGTAAGTAAGAATTTTCCAATGATTGAGGATCAAA[G/T]TTCTATTTCTGAGTTATCTGATGTCTAGATAAAAAAGGAAACTTGTTTGCTTAGAATCCA 148
Cl-05-22556 Cla97Chr05 22,556,181 AAAAGTGGTTGGTTCTATTAGTTATAGTTGGATGTATCCTATTGAAAGGAGTTTACAGAC[C/A]CTAAGCAATACGTACGAAACAAAGCACGTCCTAAGGGTTCCATTGTAGAAGCATTTATAA 149
Cl-05-23283 Cla97Chr05 23,283,247 CCATGGTGGATGCATTATCCCATACTATCTGTGATCGGTGAGTATTTTGAAGATATAGCT[G/A]AACAGGGTTAGATGAACTAAGATCCATATTCAACACTGTTAAAATAAACCAAATACATTA 150
Cl-05-24966 Cla97Chr05 24,965,787 CACACAGTTGGCAACCCAGTTCGGTGTAACCACACCTACGTTTGGGAGGCAGAGTGCCTA[A/G]ATGAAAAAAGTTTCACTAATAGAGTTAAGCGATTATAGAGGAATGCTTATCTTATGGTTA 151
Cl-05-25250 Cla97Chr05 25,250,441 AAGTGTATCAATAGTAGAAACGAGTGTAACATTATAAAGTATATTAACAATATATTAATC[T/A]AGTGCATCAATAGTATAAACGTGTGTATCATTTTAAACTATATCAACAATATATCAATCA 152
Cl-05-25968 Cla97Chr05 25,967,918 TCAAACTCACCTTTGTCATCAGTAGTTCCCTCTCCTTCTCTTGCTTCCGCTTCCCCAACT[T/C]TGTCTCATGTTCATCACTCTAGTTCACAGCCATCTGAAGCTTTTGCTATCCCATCAGGAC 153
Cl-05-26552 Cla97Chr05 26,551,836 TCCTTGTTGACCAAGATACTTGTTTCTTGGTTAAATTATGAACTTAGTTTCTAAACGTTA[A/G]AACGTTCATGTTTGTGTTTGTGTTTAGTAGGTCTAGAAAATTTAAAAATCTAAGTAGGCA 154
Cl-05-28062 Cla97Chr05 28,062,496 TAGACCTCAAAGCCAATATTGTGGAAAATAGACCACAAAGTAACAAGAAAATGAAGTTTT[C/T]TGGTGAAGGTTCAAGTCATAGACAATGATCCACAAGGAAATTCAATAACAAATGCTTCAA 155
Cl-05-31097 Cla97Chr05 31,096,911 AAAATTTGGCAGGAACGAAACCAAAGTAAAGAGAAAAAATCAAAATAGATGGAAAATAAA[A/G]AGGAAATATGATTTTTTTTTCTTTTTTCTGTCATCCAACCAAAATTATTATTCTCAATTT 156
Cl-05-32224 Cla97Chr05 32,223,829 TTTATCGCTTGAGTGATAGAAATTTATCACAGTCGTCGCTAAATTGGTAAACGTCTATTG[C/T]GTTCTATTGCTGTTAGACAATAAGTTTTTCCTATATTGTATATAGTTTGACTCATTTTGC 157
Cl-05-32990 Cla97Chr05 32,989,682 GCTAAATATATATTAAAGATTTGTACCTTATTTTTTCAAAGGTCGTAGCCTTCAAAAATT[A/G]TCAACTTTCTTGTAGTGTAGATGTCACAGAGTAAACGAGTTAATATAAATATGTTATCTT 158
Cl-05-34317 Cla97Chr05 34,317,304 TTATGGTAAGAAAGTCACCGAAAATAAACATAATTCAACTAATATAAACTACGTGCTCTA[A/G]ATTTCTATGTCAAAGGTTTGAATCTTTTCTTATATGTTGAACTTAATGGAAAAAAAAGGT 159
Cl-05-34646 Cla97Chr05 34,645,761 TATTTGTAAATATTTTAGTTCATTTTTCTATATTTGCCAACAACTCTTATTTTTTATTTT[A/G]AATATCCATGAACATCTAAAATTAGAAAATAATTTTTGGAAAATTATCTCTCTCTCCTCT 160
Cl-05-35535 Cla97Chr05 35,535,326 AACCAAAATGTTACTAATAGGAATATTATATAAAGGAAAAAAATTATCAATTTATACCAT[C/T]AATTATATTTTGTTTGAAAAAAATTGTGTAAAACTAATAATTGTATTAGTTATTCTTCTA 161
Cl-06-1343 Cla97Chr06 1,342,935 AAAATTAGACAACAAATGAAGAAATTTGGAGGTAGAAGTAATATTTATTGACTTAATTTT[A/C]AAAAAAGAAATGATTATCAAACAATGTGTTAGTTTCTCATCTTGTAAGTATAGTCTTTAT 162
Cl-06-1974 Cla97Chr06 1,973,875 TTTGTATTTTTTTTAATTTAAAAAACATGGTTTCAAATTATGATTTATTTTAGACCATAT[G/A]CTTTCAAAAAGAATATTTTGGTTTGCTGACATAAAAATGTCTATTTTATAAGTTTCTAAA 163
Cl-06-3166 Cla97Chr06 3,165,547 ACACGAAGGGAAAACAATAAGACTTATATATAAGTGATAAGCACCCTAACTTTATATCAC[C/T]TATAGCTTAAAGTGTTAATATTACAAAGTTGATAGTCATTAATAGACTTCCATCACTTAT 164
Cl-06-5828 Cla97Chr06 5,827,963 ACATCGGAGAAGTTTGGGAGAATTTAATTTCTCCCTGCTTCTCCTAATGCCACTTTAAAT[G/A]CATTAATAAACAATTGAGATTTAGGAGTCAACATGAGAATCAATAAGAAGGAAAATTATA 165
Cl-06-6884 Cla97Chr06 6,883,856 AGAAAAGTTTTGCCACATGTCAAATTGTGATTGATCATTTGTATGGATGACACTAGAAAA[G/A]ATAAATGCACCCAAGTATTTTTTTAAAGTTATTTGATTTTCTCTTATACCCCTAACATTA 166
Cl-06-7118 Cla97Chr06 7,117,610 GTAAAATTTTGAAACATGTGGACCAAATAAAAATTAAATTTAAAAGGGTAATTGGGTTGA[A/G]TAGCAGTTTTAGAGATAATAATTAAGTGTTTAATAACATTTTTAAAAAAATTACAAATAT 167
Cl-06-7473 Cla97Chr06 7,472,874 TTTGTTCCCAATAAGTTCAAACTTATCAAGAAGGAAGATCTTTGTTATTTTCCCGTAAAG[G/T]TGGTCATCAGAATTTTATGAGAAAAGTCACAAAGGGCTCAACTCCTTCCTGATAAGTTTG 168
Cl-06-9294 Cla97Chr06 9,294,157 GCAATTAGTTTATCTAATAAAAAATAATAATAAATTAATTGCAAATTGGAAGCTTTGTGT[T/C]TATGGAGGATTGGGAAATATTGAAAAATTGTTGTTAAAATTTGGAGAAGATGATGAATTT 169
Cl-06-9772 Cla97Chr06 9,772,479 AGATTAATCAATATTCATTAACTATAAGTCACTCGACTAAAGATTTATTGTTGCACTCTA[T/C]GCACTATAGATATATTTATGTGTCCACGGAATTAACTGATCAACAGTAAGCCAACCCTTC 170
Cl-06-9881 Cla97Chr06 9,880,582 TGAGAACGATCTCGTAGTATTTCAATTCCTAATACAAAGAAGCATTATCAAGATCCTTCA[G/T]CTCCAAATTCTTTTTTAGAAACTTTTTAGTGTCATGTAATAAACCTACATCATTACTTGC 171
Cl-06-9968 Cla97Chr06 9,967,668 TCAATGGTAGAAGATGAATTTTTGAACCCTACTTCTTCGTTCACTTCATCTTTTCATCAA[C/T]CGGCCATAAACAACTACATTGATCCATTTTATCTTCATCATATCGATAACACTAGCCTTG 172
Cl-06-10600 Cla97Chr06 10,599,775 ACTAACCATTTCTACAGTGACTTTTGCTTAGCTTAACAGTGATAGTCATCTAGGCTCCCC[T/C]TAGATGTGAGACTCCCTCTCAAGATTACTTAGACTCCCTCTAAGTGTAATAATATTAGTG 173
Cl-06-11594 Cla97Chr06 11,594,253 ACCGGTACACTTCCTACTTGCCATATTTCTGCTCATGTGTTATTAGATCATGGAGCTACC[C/T]ATTCTTTTGTTGCTAGCATGTTTTCTGTAAATATTGATGGTAAATTAGAACCTTTGCCTG 174
Cl-06-11617 Cla97Chr06 11,616,507 GCTGTTGATTCTAGATTGATCTAGAGATCAAGTATGTAACAATCATATAGTTTGGGTACT[T/G]ATAAGTTAAAATGCTTGTAATTTACCCCTTTTTTTTGGTTTGGTTTATAGTTCGAGTGCT 175
Cl-06-11810 Cla97Chr06 11,809,653 ATAAATATTCCACCAATTTTAATGTAAATAAAATGTTTATTTATAAAGATGGTGTTGATA[T/G]TCGTTCTATAAATTTATAGAGTAATTTATATGTGCTAAGACCGTTAGCATCTATAGCACT 176
Cl-06-11988 Cla97Chr06 11,988,031 TGTTGGTTTGTATAAGTATGATTAGTTCATTTTGAAGAATATACGTATAACGACAGGATT[C/T]AACGAAATAACAATAAGAAAAAATGTATTTCATTGACAGTAGAAAAAAATGTCGTTAAAT 177
Cl-06-12278 Cla97Chr06 12,277,922 GGTCTGTGAAAAACTTTGTGCAACTAGTCTTACTTTATATCTTCTTACCTCATTATTTTC[A/C]TTCTTTTTCTCACAAATACTCATTTGTATCCCACAAGTTTGACATCTTCCAAAAATATGA 178
Cl-06-13190 Cla97Chr06 13,190,064 TTTCGAACAAATATGTTCAAGTAAGTATGACCATCTGCTTGAGAGAGTATGTGATTAACG[C/T]TAATCATTTAACAAAAAAAGAATTGGTTTTAGAGATAAAGTTAAACTTTTTCATGAAGAA 179
Cl-06-19076 Cla97Chr06 19,075,554 TAGCTAAGTGACTTAACGTTCACAATTGGATCCTCAGTGGAACAATATTCGGACTTCGTC[A/G]TCTTATATTATAACTTGACATGTACACTTACGGTTAACACGAAAAGTGTGCATCACCTAT 180
Cl-06-19978 Cla97Chr06 19,978,154 CATGAAAAATTGAGATAATTGTAGGAAAATCCCAAAAAGATGGGTCAAAATGAGATGTGG[C/T]TCAATTCTAAAAACTAATGTTATATGACATTTTGTTTAGAGTGAAATTGTCATGTTTGTC 181
Cl-06-20306 Cla97Chr06 20,305,538 TGGTTTGGACACTCTACCCTTTACTTTCTTTGTCTATGAAAATGTTAAGGTAAGTGAAAT[C/T]CTTGTTCTTGAGAGCAAACCCATGTAATTTCTTGAGACTCTATTTTTGTATGACATCCAT 182
Cl-06-20829 Cla97Chr06 20,828,835 GTTGGGAAAGGCAAAGGCAAGGACAAGGCGGCTAACAATGTAATACCCTCTCTCCAAAAA[C/T]CTAGGTATCCAAAATGGTTAGTAAGGATAAAAGGATAAAAGATGAGGATTAACGGAACTT 183
Cl-06-22741 Cla97Chr06 22,741,226 AAAATAATTTTAGTGGTCTTTCTATCCCTACCACTATCCGCAGCCCAGTGACAAAATTTC[A/G]TAGTCATATTCCATGATTGGATGCCTCCTAACATGGTATAAACTCCGCAACCATGGTAGA 184
Cl-06-25481 Cla97Chr06 25,481,412 ATCTATTTACGATAAACTACTATAGACTATTATTTGTTTCAAGACACAGATAGCAGTCTA[C/T]CACGGTCTATCATTTTTCTATATTTGTTAAAATTAACTTTAGCAAAAATTAAACTAAAAT 185
Cl-06-27516 Cla97Chr06 27,515,738 AAAAATTATACTTTAACTAATTTCTACTCTATTATTATTGATTTATTTTTTTGACGAAAG[G/A]TTTTATATAATTCATTATTTTGACAAATTGTTTGAATTAAGCAAGGTGGACAACGGGAGA 186
Cl-06-27985 Cla97Chr06 27,984,567 TTAACAAACGTAATAACTACTTATTCTTAATCAAATAATTTTCTCACTCATTTTGAACAA[T/A]ATCTCAACTAATTGCTTGTTCTTTTTTTCTTTTTTGTAATCTTTTTTTTTTTTTCAAATA 187
Cl-06-28350 Cla97Chr06 28,349,567 AAAGGTGTGTTTCAAAGTGATTTTATTCATTTTAAAATGACTCTCAAACATATACTAAGA[C/T]TCCAAATGAGAAGGACTATAGTAGAGATAAACCGTGATAGGTTATTTTGTTAGAAACTTA 188
Cl-06-28475 Cla97Chr06 28,474,757 TCGCTCCTCTCAATACTCACTCTCACTTTCAATAATTCAACATTTGATCTCGCTATTGTT[T/C]ACGTTACTTGTTTTCGCTTCTTGTTTCGTATATTTTTAATTTGAACAGACATATAACGTT 189
Cl-06-28763 Cla97Chr06 28,763,150 TTTATTGTTAAAAATATAGACCATAACAAATACTTTACGTCAACAAATGTTATACCACGA[C/T]ACATTTTTTATTGTTACAAAATTTTGTATTTATGACACTTTTATATTGTCAGTAAAATAT 190
Cl-06-29023 Cla97Chr06 29,023,268 AAAAATGCTATTGCGGTGCGTTAAAAAGCGAATAATGCCAATTTCAAATGGTTGATAAAA[C/T]GAGAGAACTAAAGCTTTTTAAACACACACAATATAAATATATATAAGTGATCGACCAATA 191
Cl-06-29067 Cla97Chr06 29,066,679 TTAAAGGCATTTTAACACTTTTATATCAAATGTGCTCCACTTGTGTACTTGTACTTGCTA[C/T]AATTTCTTATCGTGCCTTGGTGAAGCTGAGCATGAGCATGAGCATGACATTTATGAATGT 192
Cl-06-29170 Cla97Chr06 29,170,041 CTGACGGTTCTATTGGCATCAAAGAATTCCTTCATTTGTAGAATCTCAATCTACCAACAC[C/A]AAAATACCCATGCAAGGGTTTTGTTTGTTAAGCTTAAGTTGTTGTTTTGGCTCTCTTTTT 193
Cl-06-29283 Cla97Chr06 29,282,891 CGTAAATAACACGTAAACGAATACAAATTAGGTTTTGAGAGCATTTACCCCCAAGGCTTC[A/G]CCAAGCGAGACCTCGTACTTGGTCATTTGGAGTGCCAAATAACAAATTCAAGACATTTCA 194
Cl-06-29334 Cla97Chr06 29,333,793 TTGGTAAATTTAAAATTTCAGTTACGTTCTCAAGGTTAACGGTACATGCGTACAACACAT[A/G]TTGTGTGGTAGGTTGTTAATTAATTATAGGATGTTGATGTGCTTGAGCGGAATGGGAAGT 195
Cl-07-47 Cla97Chr07 47,289 AATCAGAGAATATAATTGAAGAACATGAGTCGTTAAACCAAACAAATACAAGAATCAAAA[A/T]AAATCAATATGAGGGAAACTTCACTCACAGGGACATTGGCAAGAAAAACATCCCGAAGTA 196
Cl-07-3048 Cla97Chr07 3,048,380 TTTTCCTATATTAGGAATTCGTCAATGTAACAAAACTAAAAAATCCATAAAAGAAATAGT[T/A]TTTTCTTTTCTAGAAAAAATAAATCAAGAGACAATTTTGTTTCCATGGTTCTTCACACAC 197
Cl-07-3225 Cla97Chr07 3,225,296 AATCTAATATTTTAATACGCTTAACCGTTACAGATCTAAAATTAATTTAAAATCTAAAAG[T/G]TTTCCCTATAAATTAAGTACAATGATTTTAGTTTTAAGATAAACAAAAAATCATCTTTAC 198
Cl-07-3328 Cla97Chr07 3,327,808 AGACAATATGAGGTTTGACTTACACAAGGTAAAATCTCAGTTGCAGACCATCACGACCCT[T/A]CTTAGATCATTATGTCGAGTATGAATTTAAATAAATTAAATGTCTTAATTTTTTTTTAAT 199
Cl-07-4431 Cla97Chr07 4,430,582 ACAAGTTCATTTTCTTTCGCTCTTTTTCATAATATTTTCTTTTCACTGCAAATGATTTTA[T/C]CAACTGATTTTTAAGTAGGCATATAAATAATTTTTCTTCCTCAGTATTACATTACAACCT 200
Cl-07-4860 Cla97Chr07 4,860,190 CAAAATAGTTACCAAACAAGGTCATTGTTATAGCTATATGTAAACAACCAAGAGCTCAAT[T/C]TAACATAACTTAGTGAATAATGCTATTGGATCTCCTATTTGTCATTATTGAACTAAAAAA 201
Cl-07-5866 Cla97Chr07 5,866,213 CTTGCTATGCCATCATTTGTGTTGATTTTCTCTTCATGAGATTCAAAGAAGTTTGCTATA[C/T]TCAAATTTGTCATATTGGATGGGTCAAATATATGTATGCAAAATAAATTTTCTTCCACAT 202
Cl-07-6179 Cla97Chr07 6,178,831 TTAATATAATCTACTAGGAACTTTCGCTAGGTAAAAACATGGAGAAATCAAAACCTTGGT[C/T]ATGGTACCATAGCTAAATAGCTTATAAAAGAAACATGGTTTACTTAACATCACTTCCACA 203
Cl-07-6191 Cla97Chr07 6,191,134 AAGGTGGAAGTTGTCGTACTTAACTAAATTAATAGTCTAATCTCGTGGGTGATACTCGAA[T/C]TCAATTACTATATTAAAACTAAATACCGTATACTTGTGGTTATTCATGTTGTAAAAATCA 204
Cl-07-6288 Cla97Chr07 6,288,122 ACTTATCTTGATGAGTTATGTTATCTTATGAGGTTATACAACATATAGTTAAGTATAAAT[C/G]ATCTGTAGTTGATTAATGTTATAGTTACAGTAGGAAACAGGTGTATGCAGGTTCGCACTT 205
Cl-07-6531 Cla97Chr07 6,530,788 TCATAAAATAAGCATTTAATAATGGGACAAGATTCATGAAATAAAATAATATATCACTTA[A/G]TAACGTATCAAATGCAGAATGGAAGTAGTATATATTATGGTAGTACTAGAAATTTTCCTC 206
Cl-07-6796 Cla97Chr07 6,796,474 CTCTTCAATTAAATTAATCAACATTCATTAACTATGGGTCACTCCACGACAGACCCACAG[C/T]TGCACTCTTTGTATTATAGACATATTTATCTGTCCACGAATATAACCAATCAACAGTGAG 207
Cl-07-6834 Cla97Chr07 6,833,559 TTGTTTTGTTGTTTACGTTTTACAAGTGTTTTTAAAATTCAAGTCAAGTTTTAAAAACTA[G/A]AAGAAAAAAAATTTCTTAAAATTTGACTAAGAGTCCAAATGTTTACCTTAGAAAGATAGT 208
Cl-07-7368 Cla97Chr07 7,367,751 CTCCATCTCCAAAAGTGACCTTTCTAGTACTCATTGGTTTGATATTTGATAAGAAGGTCC[A/G]TTCTCCAGTCACATAACGAGAATGACCACTGTCAAAGTACCAGTCTCCTTGAGCAGAGGA 209
Cl-07-8705 Cla97Chr07 8,704,687 ATTGGATTTGGTTTAAAACAATGCAAACAAATTACCTAATGTAACCAAGGTGAAATTGTT[G/A]TTAGTTGGTGACGCTTAAAAAAATAAAGACAAACAATGATGGAAGTAGGGTTTCAACTAT 210
Cl-07-9070 Cla97Chr07 9,069,728 GCACATTGTCTGTCAAATTCAATATGGAAATAGTTGAATTTAATATTTTTGATGCTATGA[G/A]AGCTCCCAATCAGAGTCCTCTTATTTTATGTCAAATTGATGTGATTGATTGTTTGGAATA 211
Cl-07-12123 Cla97Chr07 12,122,843 AACAATTTGGATTTTAGTATTCAATTTGGACATGTGGCCCTTGTTTTACTTGAGATGTTT[A/T]CTGAAAGTGAATATTTGTTAGTCAACAGCCCTTTTAGCATGTTTAATTTTCATTTATAAG 212
Cl-07-12164 Cla97Chr07 12,163,928 ATTTAGGGAGTGAATGCTTAATTAAGGACTAATCCACTTATTTTGCATTCATAAACTAAT[A/T]TAAACTCAAGGGAAAAATCGAATGTTAGATTAGGCTATCCATTCTCTCATTCGTCACAAT 213
Cl-07-12445 Cla97Chr07 12,444,885 TTTATTACTTTCCTTATTTAGTTCATATTATCCATAATTCATAATTTCAATTCCAATCCT[C/T]ACATACAATTGATTCAACTCTAGAATAGCTAAGTAACTTTAACCTTCACAATTGGATCCT 214
Cl-07-17943 Cla97Chr07 17,942,591 GACAATACGTAAATAACATCAATTCAAATCATATCCAATCAAAATATGCTCAGTAGCATT[G/T]TCATACCATTTATGTTCATTTCCGAAAAAGAACATGAAAATTCAGTATATTTCAAAAACA 215
Cl-07-21306 Cla97Chr07 21,305,578 AATGTATTCCAAACACACTATTAATTAACAAATTCATTTAAATCATATTTAAATGAACGT[A/C]TCCACATAACCTATGATTTTAATTCAATTAAATCAAATTGAATTAAATTAAATAATCAAC 216
Cl-07-24773 Cla97Chr07 24,773,253 CTGTTGAAAATATTTACAATTAATAGCAAAATACACCGTTTATTTGTGATAGATCGCGAT[A/T]GACTACTATTTGTGTCTAGTAGTTTATTGCGATTTATCGTAGATAGACAGTAAAATTTTA 217
Cl-07-26217 Cla97Chr07 26,217,423 TATAATACTAGTTTTACCTTCACTCATTAGCTTAAGCTTTTGGGTCAATTGGTGAATTAA[A/G]GTGGCATTAGAGCAAGTGATTTAGAGGGATTCTCGTGTTCAAATCCTGCTTTATTATTTA 218
Cl-07-27106 Cla97Chr07 27,106,326 GACTGTAGATCGTGAGTTATTGTATGTTCACATTCTTGCTAAATTATTCATCTTTACCTT[C/T]AGAGAAAGGATTCAACAATTTAGGGGGAGCCTTATAAGTTCATCAAGTAGAGTGGTCTTC 219
Cl-07-27532 Cla97Chr07 27,532,355 TACAGTTAGTAGTTAGTAATAGACTGCAGTAGATACCATTGATAGACAACGAAATTCTTT[A/T]ATATTTGTAAATAGTTTGGTTCATTTTGCTATATTTGAAAACAAACTAATCTTTTATAGC 220
Cl-07-27570 Cla97Chr07 27,569,758 GATATTTATGTAAGAATCAACACTGAAATTTATGTGATTCATTAACAGTGTGTTAGATAC[G/A]TTTACAGGCAAAGAGTAGTACTGAAAAATATAAGATTATAGCTTCAAAGAGGCATCTCTA 221
Cl-07-29223 Cla97Chr07 29,222,661 TTGCATGATAATGAAGAATTCTTTTAGAAGTTGCTAAAAGTGGAGGATTGTGGGAATGAA[T/G]GTTAGAAATTGTTGCATAATAAAATTGAGTTATTAATTTAATCAAGTTGTTTTTCAATAG 222
Cl-07-29789 Cla97Chr07 29,788,913 TTGAACATCTATCCTCGAAGTTAAGAGCATAAGCTTTATGCCAGTTGAAGTTAAACTATG[G/C]TCTTTTTGGCATAGAGATAAGAAAATATTTAGCTCTCGTTTTGGTGGGAGATGAATAAAA 223
Cl-07-31053 Cla97Chr07 31,053,039 CCACCCTAGAAAGATTTTGATTTTTAGGTTAAATTATAAGTTTGGTCCATTAATTTTGAG[A/G]TATGCGACTTTAGTCCTTAAATTTTTAAAAGTGTAATTGATTTGTACATTTTCAATTTTT 224
Cl-07-31362 Cla97Chr07 31,361,691 AATAAATTTAGCTACAATCAGAAGGGGAAAACACTCCTGATTGTGATGTTTACAGGAACT[A/C]AGGGGGTATTGTGCACTCCCAATGCCTACCACACTGCATTTGGTTTTCCCTCTAACTTGC 225
Cl-08-1104 Cla97Chr08 1,103,941 ATATTGAGTCGGCAAACGGTCATTCTCACCCATACAAATCAAAGGACAATCCCTCGTGGA[C/T]AGAAGTTCATAATATACTAGAGTTTAAGACTAAGTTACCTAGGTCATCCAATTGGAATAG 226
Cl-08-3883 Cla97Chr08 3,883,058 ATGATGATAGTACAAATGTTAATTTTTAAGTTTCAATTTAAATAACTTTTTCTAATAAAT[T/A]AAGAAAATACAAGATTGAACAAATGACCACTTGTTTTTGTTTAGTTTGCTCCCACAAAGC 227
Cl-08-4039 Cla97Chr08 4,039,475 ACAATTAAGACGATAGAGCAATTAAAATGCATGAGTTCTCAAAATAGGTTATAAATCTAT[A/G]TTGAAATTAATTTCACATCAAGTATTAAATATTTGTGAACCCCTAATAGGCGCTATACAC 228
Cl-08-4826 Cla97Chr08 4,826,182 GATGGTATATCAATGATATTACTTAGGTAGATATCAGTGATATAACTTAGGTCAATATCA[G/A]CGATATCCATGTATTAGTGATATCTGTGATAGAACTTAGGTAGATATCATTAATAGAATA 229
Cl-08-5807 Cla97Chr08 5,807,097 CAATTAGATGGCTATCAAAAGGCTAACTAATTGACAAAAAAATAGAAGGTAATTAGATGA[T/C]AATAAAAAAGCAATCACATAACAATCAGTGAGAGCATTTTTGATTTTTCAGTTTCAATGT 230
Cl-08-6283 Cla97Chr08 6,282,920 AAAGTAGGTTAAAGTTAATTTTATCAGCGGAAAAATCAAATACTTCTCTAATAGTTAGTC[G/A]TATAATTGTTTGTTTGATCTTAATCCTTGTTTAAACCACCAATAATTCACTATTAGTACA 231
Cl-08-7112 Cla97Chr08 7,111,584 ATCATTTGATCTGTATGGGTGGAGTGGTTAGATCGCCGACTCAATAAGTCTACCATTTTG[A/G]AGATTTGTCTGAGTAAGAAACTGGGAACATATCTTCACAAGATGAAATTCACTACTTCCC 232
Cl-08-13455 Cla97Chr08 13,455,137 ATGCTTTATTCTCTTTCCGGAATTTTTCTTTTACTATCTTAAGATCATTACTTAACCAAC[G/A]CATTGTTCAATAAACTTTAATTTAAAATCTTACTTTCTGAATTTACTTTTAAACTTTTCT 233
Cl-08-14610 Cla97Chr08 14,609,736 AAATCCTCATCGGACTCATATTACATTACTATTCGTTGAGGTAACTCCATTGTTCTGTGT[C/T]TGACTACCACAAAGGGTTGAAAGAACAATGATTTTTCGTTAGTGACAATTGGCTCGCCCC 234
Cl-08-14774 Cla97Chr08 14,773,870 CTGTCTTTGCTCATTGTAATCCGTTGTGCAAAATCGTTGTTGAAGAGTAAAAGATCAAGA[A/G]GATAACTTTTATCTTAGGGGATAGCCTAAGGCATTGCTTATGATTAGAAAATGGAAGATA 235
Cl-08-15164 Cla97Chr08 15,164,014 CAATTTGAGAAAATCGAGAGAAGAAGCGTGAAATGGCGGGTTGAACATGAGCTTTTTAGT[T/C]ATTTACCAATATGACTATTATTGATAGCTGATATCAGTGACATCTAGTGCTAATTTTGTG 236
Cl-08-15390 Cla97Chr08 15,389,637 TATTCATGTTCATCATTATAAAATATTTTGAAACTTAATAGTGGTTAATTGAGGTGTTTG[C/T]TTAAATATTTTTAGTATATCTAAAAATAACTAATATATCGAATTTGAGCATTTTACTTAC 237
Cl-08-16276 Cla97Chr08 16,275,920 GTGGAGACATTTAAGTGGAGTATCTTATAGGAAGAATTTTTACAAGACCAAACAACAAAA[T/G]AACTAATCTCTCTTTGTAAATCTGTAAATTGATGAGATTAATATTTCAAAAGATGATCAT 238
Cl-08-16648 Cla97Chr08 16,648,435 CAGTTTGATCACATGATAAATGTTGAAACATAGATGTGAAAATTAGCATAATTTCAGTAC[G/A]ACTTACTTCACAGGTGGACTATGCATAAATAGAATTCAAGGGTTGAATTTAGATAGACAA 239
Cl-08-20708 Cla97Chr08 20,708,458 GAGAAGAAGAATCCAAACAAAGAAAAAAATTGAAGAAAAAATTGTAATTATGTCTAATGA[G/A]GAACAAAAGGAAGGGACGAGAACCTAGAGAAAGTAGAACGAAAAACTAGAACGAGAGTCA 240
Cl-08-21083 Cla97Chr08 21,083,001 AAGGACCAAGATCAACAAACTAATTATTCAGCACATCGACCAGAATATACTAAAGTGTAC[G/A]TAGGCGAATGCTATCTCATCGACACCTATCGATTTGGAAGAGACACGTATATTGACTAAA 241
Cl-08-21229 Cla97Chr08 21,229,314 AGTTGATCAAGTCGTTTGACTTCAGCCTCAGCCTTGAAATATTTTTCCACATAATTAGCA[G/C]TTAATTCATTAGATCTTCTATTTATCTTTCACACGTTGAATAGTAGTACCTGCTCAATTA 242
Cl-08-22345 Cla97Chr08 22,344,910 ATTCTTACAACCTTCTAATCCATGTCCTCCGTCTTCAAGAAAGGAACTGCATGTTAATTA[G/C]CTAAATTAAGTTTTTCACACTTTGTAACCATAATAATTACTTAATATAATTACTAAAATC 243
Cl-08-22538 Cla97Chr08 22,538,270 TTAGAAAATTTAACTATATATAAAGAAAATATGAATGTTTTCAAACCTAAAAAAATGAGA[G/C]AAAATATTTAGAAATACAACAAAATTTTGTGATAGACATTGATAGACGACATCTATTAGT 244
Cl-08-23444 Cla97Chr08 23,443,617 CCTTTCCATCAGGTCTGTATGAGAATGTGAAAACGGGTTGGATGTTAATTTGGATAACGG[A/G]ATTTCCAACCCTACCAATGTTTTCATATGGTGAGGTTATACCAATTATGGAATAGGGCAG 245
Cl-08-23544 Cla97Chr08 23,544,019 TGTCATCCAAAGAAGCATGATATTGCTCCGAACAATGTGGTGTTGAGCCTTGTTCCTCTT[T/G]TAAGAAAGCATCTTCAGCCAATTGGCATTCCTCTTTTGTGCGTGGAATCGTATTGGAGTA 246
Cl-08-24074 Cla97Chr08 24,074,402 GGGGAAATAAATGAAAAATTAATGTAAGATTTTCGATTAATTAATCAAGTCTCATAAGTT[C/T]ATAACTCGTAATTAATAGGTGAATAGTTGACCCTTGAACTACACACATTAATTAAATAAA 247
Cl-08-24298 Cla97Chr08 24,297,902 TAGTGATATCGGCTAGTGAGACCCACAAAAATTTCAACATAATCAGATTTAGCATTCTCC[G/A]TCCTTCACATAGAGTGCATGTTTTTATTATAGATGAGGAAGTGAGCCTCATGTATTAGTA 248
Cl-08-25040 Cla97Chr08 25,039,748 AATAAAAAAAATAAAAAATTAACGCTAATTTAAAATATAGTCGAAAACATTTGTATTTTT[G/T]AAATATTAAAATGGGGCAAATTGAAAATGAGAAAATTCAACCAAAGTGATGTAATAATAT 249
Cl-08-25407 Cla97Chr08 25,407,328 TTTGTGCCTTTTGTTTTCTGATTGATTGTGACAAACCAATGTTTATGAATTTATATTATA[T/C]TTAGTTATTTAAATTACTAACCCATTTTTCATTTTGATTATATTTTAGATTGAAATATTA 250
Cl-08-25875 Cla97Chr08 25,875,381 ATTGGTGGCATTCGTCTAAATAGAATCACTATGTTGAAATTTTAATTTCAGTTTGGTTGC[T/C]TTCATATATTATCGATAAGTGATAGCTATGTGGAAAAGATTATTTAATTGAATGAGCTAA 251
Cl-08-26011 Cla97Chr08 26,010,681 CAAATAAAGGGAGAAAAAAAAACACTCTACATACAATGAGAATTTGTGTTTGTAAATTAC[A/T]AAATTGAATATTATAAATTAAATTTAGATGTACGAAAATTGCACTCTATATTCCATTATT 252
Cl-08-26301 Cla97Chr08 26,300,851 AACTCTAAATTTAACTATGTTGTAAAACAAGAACAAGAAATGCAAATTGGAGAGAAAAGA[G/T]AGTATAGAAATGAATGTATTCTATATTCAACTATGTCTCATAAAGGCCCAATAGAGCCTA 253
Cl-08-26825 Cla97Chr08 26,825,270 ACTTTCTCACTTTTGTCCACTACACTTGGTTTGTTTGGAGTTTGAAAAACTCCGTTTGTT[G/A]CCCTAAGTCATCACATGTAGTCAGATCAAGAAGAAACAACTCCACGTTGTTATCGGAGGC 254
Cl-08-27600 Cla97Chr08 27,600,119 CTAAGATGAAGTGTTGTTGAATTATCAAACACGAAAATGTCCAAAAAAGACCATTGTCAT[A/C]ATGACACGTGTTAGTTAAATTTAATAATGTGATTTAAATTGGTTCGGTTGGACGACATCC 255
Cl-08-28029 Cla97Chr08 28,028,525 CCAAAATTACACTTGAAATACTATTAATATACTTGAACAAATGCATTGTTAAGAAGTACT[A/T]ATTTTTTTGACAATACACCAATATTACATAACCTAAAATGAATTTTGAGTTAATGTTGTG 256
Cl-09-1057 Cla97Chr09 1,056,565 ACTATACATTATATATACATAATTCACAAGTTTATTGTTACAAAGTACAAAGAACCAACA[C/G]AAAACTTAAGAAATTCAACCTCCCTCTGTGTGCAGAATATTTAATGCCAAGGGGTGGATT 257
Cl-09-3915 Cla97Chr09 3,915,363 TTATGACTTATGAGATATTAAATTGAAAAATCAAATTGAAGTAAAAATGTTGGAGTTGGA[A/G]CTTATTTGTAAGAGTATTCACATCAGTATGGTGCTGAGTTGCGTATACTTTGATGCCTTT 258
Cl-09-4081 Cla97Chr09 4,081,226 GAAGAAAGAGGGAGAGAAGAACTAGCGACTGAATTCGAAGAATCTTCAAAATTCCTGGGT[G/T]TCTTCAATCTTTGGATTTGATTTCTGTCTTCTTTAACGGTAAGATTCACCACTGTTTTTA 259
Cl-09-4414 Cla97Chr09 4,414,313 TTGGCCTTGCCCTAGTGGCGAAAAAAGGTCAAGGTCATTAGTTCAATTCGTGGTCCCTAA[C/T]TTAGGATTGAACATTCTATAAGTTATGATCCGTAAGATTACTCGAAGTGCGTATAAGATA 260
Cl-09-5698 Cla97Chr09 5,698,483 ACATATCAAGTCACATCAATCAGACATTTGAAGCACAATCATTTTAAAATTGGCACGAAA[G/A]ATACAGGAATATTGGAGCAAAAACAATAAAATACTCGTGTTTGAAAATCTATTGTCATAA 261
Cl-09-6107 Cla97Chr09 6,106,970 TTTGTTTATATGAACAAAATCAAACATTAGCCATGGTCATTCTTTTAAAGAAGATTTTTT[A/T]AAAAAAATTTTTTTAAACAAATTTGTAAATAAGTTAAAAGGTCTTGTTTAAATAGTTATT 262
Cl-09-7364 Cla97Chr09 7,363,874 TTTTTAATCATTCATGAGTCATGCATCGGTTTACTCTTTTTTTCATCTTTTATTTTTTTT[T/C]GTTTTTTGAGTTTAAAAATATGCAAAGAGGATTCGAACCTTTGATAGCTTAATCGAGGAT 263
Cl-09-7421 Cla97Chr09 7,421,012 ACTAAAAAGGTAAGATGAACTCCAATAGCACAATGAGTTTGAAACTCAATAATTTCATAC[T/C]GAATGAAAGTTCCTGTGCTATATGAAATTCCCGACAAAGCTTGAAAATGAAAATTACGGT 264
Cl-09-9053 Cla97Chr09 9,053,275 ATGATTTATCAATCAAACCTAAATTACTCATTTTGCATGCTTAATAAATTTATGAAATTT[T/C]TATTTAACACAAGGTTGAAAGTTCAAGGATTTATTAAATACGAAATTAAAAGTATAAAAA 265
Cl-09-10809 Cla97Chr09 10,808,660 ACGATCACATTAATTGAGAATTAGAGATTTCTATTAAGGAGATTCTGTTAAATTTAAATG[G/A]AATTTAATCAGTCTTACTAGTATATTCCATATATTGGTTAGAATATTTGATAGTACTCAA 266
Cl-09-11180 Cla97Chr09 11,179,563 GTAAAACGACCTGGGTCGAGTAACATCTTCAGGGGATTGGTGTCAATTATTTTTTCTATT[T/C]GCAAAATTGTTTTAATTTTATTTAGGAGACTGACTTTAATGTAAATTAATCTAAACCTAA 267
Cl-09-11724 Cla97Chr09 11,723,537 AGCTACACTTGGTTGATCTGTTGTCGACTAGAATTCTTAAGCACAAAATTCTAAAATTTT[T/G]GTCACCTTAGTTTTTTGCAGGACAACAATTTCTGTAGTAACGTGTTGATCTTCTCCACTC 268
Cl-09-16326 Cla97Chr09 16,325,633 CATGCCAAATTGTAAATATATCTGGATTCATGAACTTCGGGTTCATTGTTGCATATGTTT[C/T]AATGACTCGTATATGAGTAATAATATAATCTAGAAAATAAAGTAGGCAAACTTGTTCAAT 269
Cl-09-17300 Cla97Chr09 17,299,974 CCAGTGGTAACCAGAAATATTTGAGAAAGGTGTTTGTTTCTAATCTAAAGAAAGCGATAA[C/A]ATGTATGCTAGACTAAAATGTGGAAGATGGAAAGGCATGTGATAACTTGTAGATTTACAA 270
Cl-09-25228 Cla97Chr09 25,227,502 ACTTAAATTAAACCTTTTCATGTATTATACTAACTAAGAGATCGTTCAATTTACACTAAG[C/T]GATCGCTTAATATACAATAAACGATGACTTAATAGATATTACTAAGTAATCACTTAAACA 271
Cl-09-25346 Cla97Chr09 25,346,448 TAAAAGTTCAAAGTTTAAATTGATACAATTATTAATTCATGTCTTAAGTTGATACAACCT[C/T]GGGTTACCAATTGATATTTTTTCCTAAATTATGCAAGATATACTATTTTTTCGTTCTTTT 272
Cl-09-26722 Cla97Chr09 26,721,657 TCCCTGACAGTATGGTGACTAACAATTCCCTCAACGTTACAAAACTCATTGAACTTAGAC[C/G]CACAAAATTTCAGACTATTATCAGCTTACAACATTTTGATCTTCTTCCCAGTTTGATTTT 273
Cl-09-27436 Cla97Chr09 27,436,236 ACATCCGTGCCTATTTATTTCAACTGACATTAATGGTATGATTTCTTCTAGTGAACATGT[T/C]GAACATATGTGCATTAGTGGATACCATTATTGATATTATCATTATGTTATTTGATACTCT 274
Cl-09-28446 Cla97Chr09 28,446,480 ATTGTACAGTGTTCAATGTATACATTCGACCTGTAAGTGGCCCATTTGAGCAATTAAAAT[T/G]GAGGGATGTGATATTTTTAATGTAACACCCCCATCCTAGATATTTATATATACATCTATA 275
Cl-09-28454 Cla97Chr09 28,454,472 ATTGTTTAGGTGCGTTAGACGACTCATACATTAAAGTGAATGTTAGTGCCGTCGATCAAC[C/G]TCGATATAGGATGAGGAAGGGAGAAATTGCCACAAACATTCTTGCAGTGTGTGATCTGGG 276
Cl-09-28481 Cla97Chr09 28,481,200 TTTTGTAATTATTAGTTTTAGGAGAAATGATACATTTTGTAATTGTTTGATTTTGATAAC[G/A]TACCCATATGTGATTGAAATACTGAAGTTGTGTAGTTAGTGATTTATAGGAGATGTGTAG 277
Cl-09-28652 Cla97Chr09 28,652,226 TCACAATAACATAACATAAAATTGAGAAAAGTTCATAATATTCTCAAGGTCTACTTCTAC[A/G]TCTCCCCAACTTTTGCACGAATGATCTAAAAAATTTTAATGAACTAACTTTTTCAAAAGC 278
Cl-09-29179 Cla97Chr09 29,179,026 GTTTTAGGGTGTTGGATGATATATTATGTATTTAAGTTAATGATTTTAGTGTTTTTCATG[A/G]ATGTTGTTGAATGATATGTTTTTCAATATTATTGTGCTTTATTCTTTATTGATTGATCAT 279
Cl-09-30026 Cla97Chr09 30,026,382 GGATTAAGGTCGACCCTGATAAAGTCAAAGCTATTTTAGAAAGGCCACCACCACGCACCC[A/G]AAAAGAAGTTAAAGGACTTTTAGGGAGACTAAACTATATCGCGAGATTTATATCACATCT 280
Cl-09-30842 Cla97Chr09 30,841,984 TTACAAATAATAGCAAAATATCACAATCTATGTATAATAGATCGAGATAGACTACTATCT[G/A]TGTCTATTATTTCACAGATAGAAACAGATAGTTGTCTATTGCAACTAACAGTAAAATTTT 281
Cl-09-31455 Cla97Chr09 31,454,856 CTACAAACTCAAGTTTGTGATAGAGGTGTTCATGGGTTGGATTGGGCTGAATGACTTTTT[G/A]GACCCAACCCAACCCAATTGTTCAGATTCTAAATTTCTTCAACCCAAATAACTCTTATTA 282
Cl-09-31472 Cla97Chr09 31,472,443 TAGTTTTGAGCGAGCTAGTAATTACTTGTGGGTGGTACTTGTCTTGCATGGCTGTACAGA[T/G]AATTGTGTCGAGCTAGTCATGCACAAGCTTTGGATATAGCAGACCCACTGATACTACTTC 283
Cl-09-32794 Cla97Chr09 32,793,551 TGTTAGTCATAGATTCTGTAAGAAAAATCATCTCCCTGATCTAAGGAAATAAAAGGTAGT[G/T]AGGTGTGTGAAAGATGGGTATTCTTTTTCTTCTTTAACTAATAGATTAAACTTTTTTCAA 284
Cl-09-35480 Cla97Chr09 35,479,919 AGCATGCGTTCTGTCTTTAGAGGTCGGATGTTCAATTCTCTATCTCACAGATGTTGTATA[G/A]AAAAAGAAAAAAAAAGGACAAAAAGATTCCTTAGCATTAGCATTTACTTAGCCTGAATCC 285
Cl-09-36262 Cla97Chr09 36,262,033 AATGACATCTAATAATTTAAAATAGCATCTTATTATTAATGATGATCTAAGAAACAAAAA[T/C]TTTAAACCAATATATTGAGAAACAGGGTTTTAGTTTATATTTTTAGAAATTTCAAATATG 286
Cl-10-176 Cla97Chr10 175,751 CTTCCGAGCTTAACGAAGTATAAATCAACAATCAAAGAATAACTTGTCAGACAACTCAAA[A/G]AATATACGATAACTTAGCAATTCTATTAAAATTAAAATCCATAGAAGAATAATATAAATT 287
Cl-10-987 Cla97Chr10 987,317 CTATCTGATAGAAATGAAAAGAAACTAAGGATTCTACAAATATAACCATTTTCGTCTTTT[C/T]GAAAAAAGGGGGCAGGCAAGAAGAGAACACTTTAAAATCTTAATTTGTGAATGAAAAGTG 288
Cl-10-4796 Cla97Chr10 4,795,522 TGGTAGACCAATGAAAAGTCTTAGAAAAAGAAAAATAGACAATTGTAATAAAAGACCTGA[C/T]TAAGGATGTAAACATCCATGAAGAAACCCTGGACATGACTGATAGTCGAATTAGTAAAAA 289
Cl-10-5704 Cla97Chr10 5,703,627 TCGCTGTTCAATTGTTCTTGATATTCCTTCCAAGACTGTATGTTTACTTCAATGAATGCA[T/A]TTAAGCCTTTCTTTTTGTATTGTTCCTCTGATTTCTGTCTTCTCTTTTCTCTTTCCTTCC 290
Cl-10-8326 Cla97Chr10 8,326,026 CACTTACAAGCATGCTCTTAGGGCATATTTGATAGTGATTCTAAAATGAGCAAAATCACT[C/T]TTGTTATATTCACTCTAAAACATGAAACATGTTTTTAATCATTCAAAACCTATTTGATGA 291
Cl-10-9311 Cla97Chr10 9,310,733 GTTTTCTTTGATTTGATAAAAAATTTCTTCCGGTTAACTAATGGGGGTTTTGTGATTGTT[G/T]TGATTGCCAGAGATTTTAGATGCAAGGATGTGAACTTGCATAAAAGTAAAGCAAGAATTG 292
Cl-10-9353 Cla97Chr10 9,353,195 GTACAGAGGTGCAAGCAGTTCGGAGAAGAACTTAGAAAGGAGATTTGGACACAAGTAACA[A/G]CTAGCTTGGAGTGGATGGAATTTTTTAGACTCGTTAAAGCAATAATGAAGGTAGAGAAGA 293
Cl-10-10410 Cla97Chr10 10,409,837 TTTCTGGTTATTGCTCATTTGTTGAAGGAAACTTAGTTACTTGGCATAGTAAAAAACAAA[G/A]TGTAGTTGCAAGTAGTGCTGAAGCAAAATTTAGGGCATTAGCCCATGGTATTTGTGAAGG 294
Cl-10-11308 Cla97Chr10 11,308,417 ATTTAGGCACATCAAAACTCAAATGTGAATAGCGGATATTAATTAATTATTTCCAACCTT[G/A]CCCTATATCCGTACAAAGTGGCAAGAAAGTCACTTGCTTTAGTGAAACTCACAATAAACA 295
Cl-10-13620 Cla97Chr10 13,619,737 GGATATATGATTATTGCTTTATGATTAATGTTCAATTCTTTGCATTTGATTGCATGTTAA[T/C]TGTAGGAAGCAGTAGGTTAGGTTAGCTTGCGAATCAAATTAGATAAGTATTGATCTAACC 296
Cl-10-17631 Cla97Chr10 17,631,494 ACCTTTTGGGACTTTCAACCCATTTCTCCTCCATATAGCAATCTCTTAATACTCTAGAAT[T/C]TGAAGCCGAACCTTCCCATCTAGGTAAGATATAAATAAATTCAATATTTTGACTACAAAC 297
Cl-10-19567 Cla97Chr10 19,566,813 AGTTTCCTTCAATTCCACAGTAATGCGGTTGAATAAAATAGAGTACACTATAACTACCCT[C/T]TTGAATGAGTTATAGACTTATTAGTCTCTTATTAAAACTAAGGGACCAATCGAAAGAGTT 298
Cl-10-20356 Cla97Chr10 20,355,998 TAAAGTCCATTTCTATAGTTTTTTTACATTATTATCTAATTTGTGTGATTTAGGGTAAAA[C/A]TTAAACCCAACCTATGTGATGTGATTTCTAGGAAAAAAAAAAATGACCCATAAGGATGAT 299
Cl-10-21388 Cla97Chr10 21,387,714 TTTGTTATGACATTTCCATCTCCAATTAGATAAATAGGACCATCATCAATTCATGTCGGT[A/G]TTGTGTCATAAAATGAATCGTAATTCTTTTCTGTCTAACTATGACACTTAAAAGACTTAT 300
Cl-10-21513 Cla97Chr10 21,512,882 ACATTTTAGCCGTTAGAGCGGCAAGGGAGAACTTCAATGTCCCCTCGGTTCTGATATGAA[A/G]CATTGATTAGGGCATCAGAGAAAAGAGGAGGTGTGAGAGAAGAATACACAACATCTGGAA 301
Cl-10-22430 Cla97Chr10 22,430,262 AACTGAACATAACGTACCAAGAAAGAGACATACGCATATAAAGAGTTCTCTATTGTAATC[A/G]ATTGGCTCTATTGACTACACCCAAACTTAGCTCCTTTCTTTGGATAATGGTCTTTCCAAA 302
Cl-10-22636 Cla97Chr10 22,635,804 TTAGAAAACAGTATGTAGTCGAATTATGGGCAGACAAATTTTTATGGTAATCAATGAAGT[T/G]AATATTCGTTTATACAGTTGTTTCAATTAAATTTTTCATGTATTATACTAACTATGCGAT 303
Cl-10-26209 Cla97Chr10 26,209,170 ACTCAATCACTCAAAAGCATAATGTTCAATTAAAGAAAGTAAGAAAATAGAAACAATGAA[T/C]AAAATCATCATAGAAATTTACATGATTTACTAACAGTATGCTATGCTATGTCTAATGAGT 304
Cl-10-26794 Cla97Chr10 26,794,243 TAACTCAATTTAGGTGACATATAGAATGTGTCAAGATCATAGAGTTTGTGATTCAAATTT[T/C]CCACATTTATTATACTAAAGAAATAAAATAAAACAAACCCCAGCTTATTTATATAAATAA 305
Cl-10-27168 Cla97Chr10 27,167,868 GTACAATGTAAACGGTCTAGATGATTTTTTTATCTAGAACAAATTGCAAAAACCATATGT[G/A]AACTATAGATAGTTATAATTACACCCTCAAACTTTTAATTGTTAAAATTAAACTCCTAAA 306
Cl-10-27363 Cla97Chr10 27,362,663 GTGGAGTTGTGATTTAACTCCACTCCTTGTTTCGCTCAAGGAGTTCATCGGTTCCACAAC[T/A]AAAATACATCAATTTTATGTATTGATTTAACTCCTTATGTTTTGTGGCTCAGGAGTTCAC 307
Cl-10-28749 Cla97Chr10 28,748,575 TAAAAATGTCTCTCACTTTGTATTCAAGTTATATAACTTTTTTTCATGTTATCAATTTCA[A/T]TAGTAATGTTAAAATGTAAAACTTATAGACCAAGTCATAAAATTTAAAGATTAAATAGTA 308
Cl-10-29297 Cla97Chr10 29,296,711 TGCCCAGCACACTAACTTTTTTTAATTATGATTATTATTATTTATAGAATTTTTATACAT[G/C]GATGATGATATTTGAAAGTTTTATTTGTTGCACGACCTACTCGAATTATTACCTCTTCTA 309
Cl-10-31629 Cla97Chr10 31,629,301 TTTGAAAAAATAATCTTTTCCCTACATTTTTATATGTTATTTTCTACCCTTCATATACCT[G/A]TTTTGAGGCCCAATTTTAACCATTACAATCTATAAATTTGAAGGTTTATTTAATTTCATT 310
Cl-10-33565 Cla97Chr10 33,564,971 TAGAACTAAACTAAAAATGAATTAGGTTAAAGAATATTAAAATACCTCGAATTAAATTGA[A/G]AAATTTAGGGCGAGTCATTAATGATCTTCTTCTTCGAACTCTAAGGTTAAATCTCAAAGG 311
Cl-10-33916 Cla97Chr10 33,915,845 TAAATATTTAGAAAAATTAATTAAAAAATCTACATGTATGCATTCTTTTCATTTCATTGT[C/T]GAGGCAAAGTCGCATTCATCAACTACCCTCTATTTTCTTTGTATCAAATAACATTTTGCT 312
Cl-10-34518 Cla97Chr10 34,518,435 TTGGATTTTAATAGTTTTTTCAATTTTTTAATAAAAAATTACTTCCATCAACCCTAATAT[A/T]GAGGTTTTACTAATGTTGTAATTTTCTTAGAACTAATTTAAGATTTTACTAAAATTTTGA 313
Cl-10-34576 Cla97Chr10 34,576,180 GTTTTCTCTGATGTCATGTAGATGACGGAGTCAGGTATCAGAATGAAGAACAATTTCATC[A/T]ATTTGATTGATTTCCGAATTTTCAAGATCCTTAGAATTTGATGATAGTATGTGCGTGATT 314
Cl-11-3800 Cla97Chr11 3,799,990 ATTTATTCCACCTCTATAAGGGTTTGTTATCTAATTTTTACCAATTTTTTCAACAACCAA[A/G]CTCTATTTTATAAACTAAAAAAAGTAGCTTTTAAAACTTATTTTTATTTTTGAAATTTGG 315
Cl-11-5229 Cla97Chr11 5,229,121 TTACATTACAAGACGAGTCCATTAGAAGCCCCTAAAAGGACCACATGGTATACAAATATC[G/C]ATATGTGATGTACACTACAAGTACAATTGAAAAAATGACAGAACAAACTGTGCAGTTTCT 316
Cl-11-5386 Cla97Chr11 5,385,981 AAAATTGAGGTAGAAAGAAAAACAAACCAAAAGGTAGGACATAAATTTTCTTTAGAAAAA[G/A]AAATATATGACCTGTTTGATAACATTCATGTTTCCTGTTTCCTGTTTTTCTTTCCTTGTT 317
Cl-11-5940 Cla97Chr11 5,939,632 TTTAATGAAATTTATGCCCTTTCGAGAGCAATTAGAGTTTAAATTTCATAATATTCACAT[T/C]TCAAAATAGGGTCCAATTGCTTAGCTCGTTGGACAACAGACGAGTCTAAATCTCATTGTC 318
Cl-11-7417 Cla97Chr11 7,417,022 TGCACAGTTTTATTCTATGACTTATACTTTAGTCTAAAAGGTTGGTAAGTGATGAATAAT[A/G]AAAACTAAACGTGATAAGATATTTGTAATATAGTTTAACATGTGAGCCATATGATGCGAT 319
Cl-11-12000 Cla97Chr11 12,000,566 CGAGGATGAGTTTTCTTCACTTGCACGAAGGGAATTTAATGTAGTTTATTATGTTCAACA[A/G]CTGTTTTTTGAAATTAATAGCAGTGTAATTCTACCTTTTCATACTTCTTTGTAATTTTCA 320
Cl-11-18297 Cla97Chr11 18,296,923 TTATTATCTATGAGATTTGAATCATCATGTTTGCCAGATTATACAAAACACCCTCTTTTT[A/T]CTTGTCTGCTTTCATTCTTTAAACGAACAAGAATAAAACTTTCCATTGAAATGAAGAAAA 321
Cl-11-18527 Cla97Chr11 18,527,293 CAATTAGATTGAGATTTGTTAGTTTTAAGTTCAGAGTCTACTTTTTATAATCCAAATTTT[G/T]TCTAATTGTTGGGAAACAATTTTTGATGTGTTCGAGAGTTGTTTGTTCATACGCATTTTT 322
Cl-11-20102 Cla97Chr11 20,102,424 AGAATAATTTATATACTCCATTTAATAAGTCTACGTTGGAGACAGAGAGTTCACTTCACA[A/G]TACAGAATTTACTTTTTCTCTATAAAGAAAGTAGATAAGTAGTTTCCTTAAATTTTTTAT 323
Cl-11-20967 Cla97Chr11 20,967,251 TAGTGCGACATTACAAAGGAAATTGCCTCCAAAACTAAGGATCCACAGAGTTTCTCTATC[C/A]CTTGTCATTTAGGCAATAATAAGATTACTAAAGCACTATGTGATCTTGGAGCTTCCATTA 324
Cl-11-21069 Cla97Chr11 21,069,109 TGTAGATTTTGAATATTTATCTAATCCACACAAAGCTAGATCTCAAACAGGTTATCTGTT[C/T]ACATTGGGAAGAATTGCTATATCATGGCGATCAGTGAAACAGACTATAACGACTACTTCC 325
Cl-11-22149 Cla97Chr11 22,149,141 TCTCACCCACTTTTACTATTAAATTTCTTTAATTCATTTTCTACTCTCAAATTTATCTAC[C/T]CAATCTACTCTCTTACGTTATATCATGTTATTTGTTAATGATCAATTTAGACCTACAATG 326
Cl-11-23067 Cla97Chr11 23,066,694 ATCACGTGCAGCACTACAACTTTGTCAAAATTTGCTTCCTATTGATTTTAGCCCCGCGCA[T/C]GACGCTCTACCTTAGGGGAAATTTTGTAATTTTTTACTCTTTTTGGCCTAATTGCTTCAT 327
Cl-11-23124 Cla97Chr11 23,123,643 TTAGTTGCCATGAAGTAGGAAATTACCAAAGCTTTTATGATATAACATGCTTGTATGAGA[T/C]TTTCATGCTTAAAAGTAGAAATTCCCAAGACCTTTACGATATAGCATGCTTGCATGAGAT 328
Cl-11-23331 Cla97Chr11 23,331,001 CTCAAGTAATAACCTAAAAGGTTTGCAGTATATGGATAAGGTTGGGTGTTTTATCCTTGT[A/G]ACACTACAGATACAACTCACTTTGTAAATGTTACAAGAGTTGTAAGTGTTACAAATGATC 329
Cl-11-24994 Cla97Chr11 24,994,068 ACTGATTATATATACTTGAATTGCATTGACATGCTAGGCGAATTTAGCAAAAAAATAATT[A/G]AATAAATAAAAATGAAATTGAAAATTTGCATGTAATTTATGTTTATGGTACAAATTATTT 330
Cl-11-25339 Cla97Chr11 25,338,645 TGGCTAAGTACACAAATTTCACGCACACCTACATGTAATTGGAGATGAGAAGGCAACCAA[G/T]ATGACTTTTATAAAATGAAAGTAAAAGATCAATTTATACTTCTAAACTTTAGGTATTGTG 331
Cl-11-25550 Cla97Chr11 25,549,773 CTTTCCCACTATTTATCCAGATGAGTCAAGGGTTAGAAATATTGGTAGACCATGTACTTC[T/C]CGAATACCGAACGAGATGGATTGGAGGGAGGTAGAAGTATTGGTAGACCATGTACTTCCC 332
Cl-11-25798 Cla97Chr11 25,797,937 TCTTCTCTCAATTCTTTTATATTCTTCTACTCAAAAGTAATTTTTGTGAGTTTAAGCCTC[T/G]CTTTATAAGCTGATGTTTGCTTAAGTTTTTACCACAAATTTGTAACTGATTTTGTTTCAT 333
Cl-11-27425 Cla97Chr11 27,425,015 CTTTAGATGTTTAGTCGTTATAAACACTTTGTTGATTTTTTAGAACCACTAAAAATAATC[T/G]GTTATTCTATAATATTTTTTCATGATAAGACTTACAGGTAATTCTTGGTTATACTTTATT 334
Cl-11-28133 Cla97Chr11 28,133,280 TATACAAAAATTTCAGTCATAAAAAGAACTATAGAAGTCTATCGTGTTCTGTTGCTAAAA[T/A]TTTTTCATATTTGTAAATAGTTTAATATTTTTTCCATTTTATCATAATTTCCTTTTGATT 335
Cl-11-29805 Cla97Chr11 29,805,372 ACCCAGGTAGAATTCCAAAATCAAATTTCATCTTAAAAAAGTTTGAGCTTGGATTCAAAA[C/T]CTTGAATAGTTAAAATACTATTTTACTTTTTTTACTTTGGAGTTTGTTTAATTTTAGTTT 336
Cl-11-30047 Cla97Chr11 30,047,221 AGACTTAATTAACTACTTATATTTGTCCATTGACTTTATTTGGTGATAGAAGTTTATCGC[C/T]GATAAAATTCAATCAGGATATGTGTAGTTTATTAGTGATAGAAGCCTATCATTGATAGAC 337
Cl-11-30282 Cla97Chr11 30,281,712 TAAATGACCAATGCAGGGTTCAAACAATAGTATTCAAGTCAAACATGGAAAATTTTGGTA[G/A]TTAAAAATAGAACGCATGACGTTCAATGGTCGAGACCTCCTACTATTACACCAAACACAT 338
Cl-11-30393 Cla97Chr11 30,392,950 ACGTTATTTGTTATTTGTTTTTAGTATATATAAATTAGAAAGTAAAAAATCGTAGAAGGC[G/A]GTGGGACCCGCTTTCTCAGGCTAAACGCGTCGACAGGGTTCGGTGGTTGGGTCACGCGGT 339
Cl-11-30555 Cla97Chr11 30,555,349 ATTAATAAATTAAGGTCGGTTCTTATTAATATTCATCTATTTGAGTCCAACCTAACCCAA[T/C]CCAAATCATAAGAATTGAGTTGGGTTAATCAGATTTTTTTATGGGGTATTTTAACACCCC 340
Cl-11-30724 Cla97Chr11 30,724,350 ATGTATGTTGTGCTACAAAGGATACTGAGTGGTTAAGAAAGAATAGGAAGTAAACTTTCA[A/T]GGAAAATCATATTCTGAAGTAAAATATTGCTTCTGACAGAAGGATTAGAGTAAAAAAACT 341
상기 단일염기다형성 (single nucleotide polymorphism, SNP)은 DNA에서 한 개의 뉴클레오티드의 삽입, 소실, 또는 치환이 일어나는 것을 말한다. '다형성'이라는 용어는 군집 내에서 변하는 유전자의 서열에서의 배치를 지칭한다. 다형성은 상이한 "대립유전자"로 구성된다. "대립유전자"는 한 쌍의 상동염색 체에서 서로 대응하는 부위, 즉 상동하는 유전자자리에 위치하는 대립형질에 대응하는 유전자를 말하며, 같은 염색체 위치 (same chromosomal locus)를 점유하는 한 유전자의 둘 또는 그 이상의 선택적인 형태 (alternative forms) 중 하나를 뜻한다. 이러한 다형성의 배치는 유전자에서의 그의 위치 및 그에서 발견되는 상이한 아미노산 또는 염기에 의해 확인될 수 있다. 이러한 아미노산 변이는 2개의 상이한 대립유전자인, 2개의 가능한 변이체 염기, C 및 T의 결과이다. 유전자형은 2개의 다른 별개의 대립유전자로 구성되기 때문에, 여러 가능한 변이체 중 임의의 변이체가 어느 한 개체에서 관찰될 수 있다 (예를 들어, CC (A로 표시), CT (H로 표시) 또는 TT (B로 표시)). 개개의 다형성은 또한 당업자에게 공지되어 있다.
본 발명에 있어서 단일염기다형성은 서열번호 1 내지 341의 염기서열에서 각 염기서열의 61번째 염기에 위치한 것일 수 있다.
상기 61번째 염기는 서열번호 1 내지 341번 중 어느 하나의 염기서열 상에서 61번째 염기이다. 서열번호 1 내지 341번의 염기서열은 각각 수박의 염색체 상에 존재하는 단일염기다형성 및 측면 서열(Flanking sequence)을 포함한다. 상기 서열번호 1 내지 341번에서, 각각의 61번째 염기는 수박의 염색체 상에 존재하는 단일염기다형성으로, 육종에 유용한 형질과 연관되어 있는 것을 선별한 것이다. 상기 단일염기다형성의 대립유전자형을 분석하면 양친과 자손의 염기 차이를 비교하여 원하는 형질을 가지는 동양계 호박을 선별하는데 유용할 수 있다. 여교배에 이용하는 경우, 상기 341개의 단일염기다형성 중 전부 또는 일부에 대해 반복친과 여교배 자손의 단일염기다형성 유전자형 분석 결과를 비교하여 반복친과 유사성이 높은 여교배 자손들을 선별함으로써 여교배의 세대진전 횟수를 감소시킬 수 있다.
상기 제제는 선택된 단일염기다형성에 해당하는 염기를 포함하는 연속염기와 동일하거나 상보적인 염기서열로 이루어진 폴리뉴클레오티드를 포함할 수 있다. 상기 연속 염기는 5 내지 121개의 염기로 이루어질 수 있다. 예를 들면, 상기 제제는 서열번호 1 내지 341번의 염기서열 중 어느 하나에서, 61번째 위치한 대립유전자 중 어느 하나의 염기 및 이의 측면 서열의 전부 또는 일부로 구성된 폴리뉴클레오티드, 또는 이와 상보적인 서열로 구성된 폴리뉴클레오티드일 수 있다. 각 서열번호의 염기서열은 61번째 염기에 단일염기다형성이 위치하고, 61번째 염기를 기준으로 상류(upstream) 및 하류(downstream) 방향으로 각각 60bp의 측면 서열(flanking sequence)이 SNP에 연결되어 있다. 각 서열번호의 염기서열에는 1개의 단일염기다형성이 위치한다. 상기 연속 염기의 길이는 SNP 유전자형 판단 방법에 따라 길이가 달라질 수 있다.
일 구체예에 따르면 상기 제제는 검출 또는 증폭하고자 하는 폴리뉴클레오티드에 해당하는 서열번호의 61번째 염기에 위치한 단일염기다형성을 식별하기 위한 것일 수 있다. 상기 제제는 서열번호 중 어느 하나의 61번째 염기에 해당하는 단일염기다형성 대립유전자형 중 어느 하나의 염기 및 이와 연속적인 측면서열의 전부 또는 일부를 포함하는 프라이머일 수 있다.
일 구체예에 따르면 상기 제제는 프로브, 프라이머 및 이들의 조합 중 어느 하나일 수 있다.
용어 "프로브"는 특정 표적 서열에 특이적으로 결합하는 폴리뉴클레오티드를 말한다. 상기 프로브는 단일염기다형성 부위를 포함하는 폴리뉴클레오티드에 상보적이거나 특이적 결합을 이룰 수 있는 것일 수 있다. 상기 프로브는 단일염기다형성의 염기 차이에 따라 결합력의 차이가 있는 것일 수 있다. 상기 프로브는 라벨링(labeling)되어 있어서 특정 핵산의 존재 유무를 확인할 수 있는 것일 수 있다.
용어 "프라이머"는 중합효소에 의한 뉴클레오티드의 중합반응에서 개시점으로 작용할 수 있는 단일가닥의 폴리뉴클레오티드를 의미한다. 상기 프라이머는 선택한 SNP 분석 방법에 따라 통상 기술자에게 알려진 방법을 이용하여 제작할 수 있다. 상기 프라이머 서열은 서열번호 1 내지 341번의 염기서열 중 일부 연속염기와 동일하거나 상보적일 수 있다. 상기 프라이머의 길이는 다양한 인자, 예를 들어 온도 및 프라이머의 용도에 따라 달라질 수 있다. 예를 들어 HRM(High Resolution Melting) 분석용 프라이머인 경우, 다형성 위치의 염기를 포함하여 HRM 분석에 용이한 크기(예를 들어, 50 내지 500bp 크기)의 증폭산물을 생성할 수 있는 프라이머쌍일 수 있다.
상기 프라이머는 플루이다임(Fluidigm) SNP 분석용 프라이머일 수 있다. 플루이다임(Fluidigm) 시스템을 이용하면 2 가지 대립유전자형인 SNP를 대량으로 신속히 검출할 수 있다. 플루이다임 시스템은 프라이머로서 LSP(Locus Specific Primer), ASP(Allele Specific Primer) 1, 및 ASP 2를 사용하고 선택적으로 STA(Specific Target Amplification Primer)를 사용한다. 상기 ASP1 및 ASP2는 단일염기다형성의 대립유전자에 대응하여 제작될 수 있다. 상기 ASP1 및 ASP2의 5' 말단 또는 3' 말단은 각각 분석하고자 하는 단일염기다형성의 대립유전자와 동일 또는 상보적인 염기를 가지는 것일 수 있다. 플루이다임 SNP 분석은 프로브로서 FAM, HEX를 사용할 수 있다. FAM 및 HEM은 각각 ASP1 및 ASP2 중 어느 하나에 연결된 것일 수 있다. 예를 들면, ASP1 및 ASP2는 SNP의 2개의 대립유전자에 각각 특이적으로 혼성화되어 결과물이 증폭되고, ASP 1 및 ASP2에 연결되어 있는 FAM, HEX 서열의 동반 증폭에 의해 SNP의 유전자형을 확인할 수 있다. 플루이다임 시스템용 프라이머는 플루이다임사에 주문하여 제작할 수 있다.
본 발명의 다른 일 양상은 상기 조성물을 포함하는, 수박의 여교배 세대단축 품종 식별용 키트를 제공한다.
본 발명의 일 구체예에서 상기 키트는 PCR 키트, 마이크로어레이, 또는 플루이다임 SNP 분석용 키트일 수 있다.
상기 PCR 키트는 상기 단일염기다형성을 검출할 수 있는 프라이머 세트를 포함하는 것일 수 있다. 상기 PCR 키트는 DNA 중합효소, dNTP 혼합물 및 PCR 완충 용액을 더 포함하는 것일 수 있다. 상기 DNA 중합효소는 예를 들면, E.coli DNA 중합효소 I의 클레나우(klenow) 단편, 열안정성 DNA 중합효소 또는 박테리오파아지 T7 중합효소인 것일 수 있다. 상기 PCR 완충용액은 KCl, Tris-HCl 및 MgCl2를 함유하는 것일 수 있다. 또한 상기 PCR 키트는 증폭 산물의 확인에 필요한 구성 성분을 포함할 수 있다. 상기 PCR 키트는 안내서를 포함하는 것일 수 있다. 상기 안내서는 키트 사용법, 예를 들면, PCR 완충 용액 제조방법, 제시되는 반응 조건 등을 설명하는 인쇄물일 수 있다.
상기 마이크로어레이(microarray)는 기판 표면의 구분된 영역에 상기 단일염기다형성을 검출할 수 있는 폴리뉴클레오티드, 예를 들면 프로브가 높은 밀도로 고정화되어 있는 것일 수 있다.
상기 플루이다임 SNP 분석용 키트는 플루이다임 시스템용 프라이머를 포함할 수 있고, 구체적으로 STA, LSP, 및 ASP 1&2 프라이머를 포함할 수 있다. 플루이다임 SNP 분석용 키트는 DNA 중합효소, dNTP 혼합물 및 PCR 완충 용액을 더 포함하는 것일 수 있다. DNA 중합효소 및 PCR 완충 용액은 상기 PCR 키트에서 설명한 내용을 참조하여 이해될 수 있다.
본 발명의 다른 일 양상은 a) 반복친 수박 시료로부터 폴리뉴클레오티드를 분리하고, 서열번호 1 내지 341번으로 표시되는 각각의 염기서열의 61번째 위치에 존재하는 단일염기다형성 전부의 유전자형을 결정하는 단계; b) 여교배 후대 수박 시료로부터 폴리뉴클레오티드를 분리하고, 상기 a)단계의 단일염기다형성의 유전자형을 결정하는 단계; c) 상기 a)단계에서 결정된 반복친의 단일염기다형성 유전자형과 상기 b)단계에서 결정된 여교배 후대의 단일염기다형성 유전자형을 비교하는 단계; 및 d) 여교배 후대 중에서 반복친과 단일염기다형성 유전자형이 일치하는 비율이 높은 여교배 후대를 선별하는 단계를 포함하는, 수박의 여교배 세대단축 후대 선별 방법을 제공한다.
수박의 여교배 세대단축 후대 선별 방법은 도입 친 수박 시료로부터 폴리뉴클레오티드를 분리하고, 서열번호 1 내지 341번으로 표시되는 염기서열 중 어느 한 서열의 61번째 위치에 존재하는 단일염기다형성으로 이루어진 군에서 선택된 하나 이상의 단일염기다형성의 유전자형을 결정하는 단계; 도입친의 단일염기다형성 유전자형과 여교배 후대의 단일염기다형성 유전자형을 비교하는 단계를 더 포함할 수 있다.
일 구체예에 따른 수박의 여교배 세대단축 후대 선별 조성물은 단일염기다형성 유전자형 차이를 식별함으로써 형질 또는 형질과 관련된 유전형의 차이를 판단할 수 있으며, 분자표지 이용 여교배(MAB) 또는 품종 판단 등에 이용될 수 있다.
일 구체예에 따른 수박의 여교배 세대단축 후대 선별용 조성물을 수박 여교배에 이용하면, 반복친과 유사한 SNP 유전자형을 가지는 개체를 효과적으로 선별할 수 있다.
일 구체예에 따른 수박의 여교배 세대단축 후대 선별 조성물을 이용하여 동양계 호박의 SNP 유전자형의 유사성을 판단하면, 수박 형질의 유사성과 연관될 수 있으므로, 수박 형질의 유사성을 판단하는데 필요한 시간과 비용을 절감하거나 판단의 정확도를 높일 수 있다.
도 1은 30개 수박 핵심계통 유연관계를 분석한 결과이다.
도 2는 여교배 집단 28 개체의 회복률 (%)을 나타낸 것이다.
도 3은 수박 육성계통의 고정도 분포를 나타낸 것이다.
도 4는 여고배 세대단축을 위한 수박 341개 마커세트의 물리적 위치를 나타낸 것이다.
이하 하나 이상의 구체예를 실시예를 통하여 보다 상세하게 설명한다. 그러나, 이들 실시예는 하나 이상의 구체예를 예시적으로 설명하기 위한 것으로 본 발명의 범위가 이들 실시예에 한정되는 것은 아니다.
실시예 1: 재료 및 방법
1-1. 시험 재료
국내외 수박 품종 육성에 활용하는 우량 계통 30자원을 형태적 특성(과피색, 호피무늬, 과육색 등)의 다양성을 기반으로 선발하였다. 국내 수박 육종 종자회사(A사, B사, C사)에서 각 9점씩 분양하였으며, 국립원예특작과학원 보유 우량 계통 및 병저항성 계통 3점을 활용하였다.
개발한 마커세트 검정을 위해 원예특작과학원 보유 우량계통과 병저항성 계통간 교배로 육성한 BC1F1 집단 28개체 및 육성계통 244계통을 활용하였다.
1-2. 수박 핵심계통의 re-sequencing 및 SNP 탐색
수박 잎을 파쇄한 후 CTAB 방법으로 DNA를 추출하였다. QC를 통과한 DNA는 Illumina사에서 제공한 방식에 따라 sequencing library 제작에 활용하였다. 이후 Illummina HiSeq X ten 기기에서 염기서열을 분석하였다.
수박(Citrullus lanatus) 97103 (v2) 표준유전체 서열 정보를 기반으로 BWA, SAMtools, GATK 프로그램을 활용하여 SNP를 탐색하였다.
1-3. 유용 SNP 선발 및 Fluidigm 마커세트 개발
탐색한 SNP 중 다형성지수가 높고, 동형접합형의 유전형 비율이 높은 SNP를 선발하였다. 염색체에 고르게 분포하는 후보 SNP를 선발하여 SNP 양 옆 120 bp 서열정보를 획득한 후 Fluidigm 사 제공 D3 프로그램에 입력하였다.
D3 프로그램으로 확인한 Fluidigm® EP1TM에 적용 가능한 SNP 리스트 중 1 Mbp 이내에 3개 이상의 SNP가 연속 분포하는 경우 중간 위치의 SNP를 제외하였다. 제외한 SNP 만큼 염색체의 시작이나 끝, SNP 밀도가 매우 낮은 지역의 SNP를 추가하여 최종 384개 SNP 후보를 선발하였다.
선발한 384개 마커세트의 SNP 유전형 정보를 바탕으로 30개 핵심계통의 계통도 분석을 Darwin 프로그램을 이용하여 수행하였다.
1-4. Fluidigm 마커세트 효용성 검정
384개 마커세트는 Fluidigm 사 제공 실험방법에 따라 96.96 EP1 genotyping을 실시하였다. 분석한 유전형 결과를 바탕으로 BC1F1 여교배 집단의 반복친 유전형 회복률을 계산하였다. 육성계통의 고정도는 유전형이 분석된 마커 중 동형접합형을 지닌 마커의 비율로 계산하였다.
실시예 2: 연구 결과 - 수박 핵심계통 re-sequencing을 통한 SNP 선발
수박 30개 핵심계통의 re-sequencing 결과 시료 당 평균 22×의 시퀀싱 리드를 확보하였다 (표 2).
샘플정보 File Name No. of reads Average read length(bp) Total length(bp) Genome coverage
N002 N002_1.fastq 26,059,347 151 3,934,961,397 18.52
N002_2.fastq 26,059,347 151 3,934,961,397
N004 N004_1.fastq 26,974,436 151 4,073,139,836 19.17
N004_2.fastq 26,974,436 151 4,073,139,836
N007 N007_1.fastq 26,264,419 151 3,965,927,269 18.66
N007_2.fastq 26,264,419 151 3,965,927,269
N010 N010_1.fastq 26,102,982 151 3,941,550,282 18.55
N010_2.fastq 26,102,982 151 3,941,550,282
N011 N011_1.fastq 27,860,734 151 4,206,970,834 19.8
N011_2.fastq 27,860,734 151 4,206,970,834
N015 N015_1.fastq 25,203,602 151 3,805,743,902 17.91
N015_2.fastq 25,203,602 151 3,805,743,902
N017 N017_1.fastq 22,899,537 151 3,457,830,087 16.27
N017_2.fastq 22,899,537 151 3,457,830,087
N021 N021_1.fastq 23,345,573 151 3,525,181,523 16.59
N021_2.fastq 23,345,573 151 3,525,181,523
N022 N022_1.fastq 32,856,505 151 4,961,332,255 23.35
N022_2.fastq 32,856,505 151 4,961,332,255
P023 P023_1.fastq 26,893,448 151 4,060,910,648 19.11
P023_2.fastq 26,893,448 151 4,060,910,648
P026 P026_1.fastq 32,884,841 151 4,965,610,991 23.37
P026_2.fastq 32,884,841 151 4,965,610,991
P028 P028_1.fastq 30,340,747 151 4,581,452,797 21.56
P028_2.fastq 30,340,747 151 4,581,452,797
P029 P029_1.fastq 31,119,072 151 4,698,979,872 22.11
P029_2.fastq 31,119,072 151 4,698,979,872
P035 P035_1.fastq 26,592,372 151 4,015,448,172 18.9
P035_2.fastq 26,592,372 151 4,015,448,172
P037 P037_1.fastq 27,765,067 151 4,192,525,117 19.73
P037_2.fastq 27,765,067 151 4,192,525,117
P042 P042_1.fastq 27,379,075 151 4,134,240,325 19.46
P042_2.fastq 27,379,075 151 4,134,240,325
P043 P043_1.fastq 28,434,300 151 4,293,579,300 20.21
P043_2.fastq 28,434,300 151 4,293,579,300
P044 P044_1.fastq 54,183,272 151 8,181,674,072 38.5
P044_2.fastq 54,183,272 151 8,181,674,072
A046 A046_1.fastq 54,640,250 151 8,250,677,750 38.83
A046_2.fastq 54,640,250 151 8,250,677,750
A047 A047_1.fastq 41,721,942 151 6,300,013,242 29.65
A047_2.fastq 41,721,942 151 6,300,013,242
A049 A049_1.fastq 26,309,492 151 3,972,733,292 18.7
A049_2.fastq 26,309,492 151 3,972,733,292
A050 A050_1.fastq 23,995,767 151 3,623,360,817 17.05
A050_2.fastq 23,995,767 151 3,623,360,817
A051 A051_1.fastq 28,453,262 151 4,296,442,562 20.22
A051_2.fastq 28,453,262 151 4,296,442,562
A052 A052_1.fastq 30,074,761 151 4,541,288,911 21.37
A052_2.fastq 30,074,761 151 4,541,288,911
A053 A053_1.fastq 69,199,585 151 10,449,137,335 49.17
A053_2.fastq 69,199,585 151 10,449,137,335
A054 A054_1.fastq 27,369,377 151 4,132,775,927 19.45
A054_2.fastq 27,369,377 151 4,132,775,927
A055 A055_1.fastq 40,841,021 151 6,166,994,171 29.02
A055_2.fastq 40,841,021 151 6,166,994,171
H056 H056_1.fastq 26,600,458 151 4,016,669,158 18.9
H056_2.fastq 26,600,458 151 4,016,669,158
H057 H057_1.fastq 35,315,203 151 5,332,595,653 25.09
H057_2.fastq 35,315,203 151 5,332,595,653
H058 H058_1.fastq 21,426,252 151 3,235,364,052 15.23
H058_2.fastq 21,426,252 151 3,235,364,052
Total 30ea 1,898,213,398 - 286,630,223,098 674.42
Average - 31,636,890 151 4,777,170,385 22.48
수박(Citrullus lanatus) 97103 (v2) 표준유전체 기반 SNP 탐색을 수행하였다. 전체 30개 핵심계통 중 1개 계통 이상에서 다형성을 보인 raw SNP 수는 5백만개 이상이었으나, 염기서열 밀도(DP 값), 다형성 지수(PIC) 등의 필터링 옵션을 적용한 결과 총 12,674개의 유의한 SNP를 탐색하였다 (표 3).
Filtering option Number of SNPs
Raw SNPs 5,834,196
Total sample QUAL >=30 5,504,914
Each sample DP >= 5 5,241,871
PIC >= 0.3
Callingrate>=90%
Homozygosity>=90%		 12,674
탐색한 SNP 중 전체 11개 염색체에 고르게 분포하면서 Fluidigm 마커 적용이 가능한 SNP 384개를 선발하였다 (표 4). 각 염색체 당 30-38개 SNP가 분포하였다.
Chr Length of Chr No of SNPs No of Fluidigm assays Successice marker Average distance between markers (bp)
Cla97Chr01 36,935,898 1,232 37 34 1,086,350
Cla97Chr02 37,915,939 1,123 36 33 1,148,968
Cla97Chr03 31,872,261 1,029 35 34 937,419
Cla97Chr04 27,110,815 755 33 24 1,129,617
Cla97Chr05 35,887,987 1,621 38 36 996,889
Cla97Chr06 29,507,460 1,594 35 34 867,866
Cla97Chr07 31,939,013 1,166 36 30 1,064,634
Cla97Chr08 28,201,227 894 34 31 909,717
Cla97Chr09 37,727,573 1,424 36 30 1,257,586
Cla97Chr10 35,099,344 1,134 34 28 1,253,548
Cla97Chr11 30,886,124 702 30 27 1,143,931
Total 363,083,641 12,674 384 341 1,064,761
최종 선발한 384개 마커세트를 활용하여 30개 핵심계통의 유연관계를 분석하였다 (도 1). 각 계통의 주요 과실 특성(과색, 과피색, 과육색 등)과 계통도와의 상관성은 확인할 수 없었으나, 384개 마커세트로 핵심계통이 유전적으로 다양함을 분석하였다.
Fluidigm 기반 대량마커세트로 전환하여 1개의 여교배집단(BC1F1) 28개체에 적용하였다. 각 개체의 우량계통 유전형의 회복률은 73.7-100%로 평균 81%였다 (도 2).
수박 육성계통 244점에 마커세트를 적용한 결과, 전체 384개 마커세트 중 341개 마커세트가 동형접합형과 이형접합형 유전형을 모두 보였다. 해당 마커세트로 확인한 육성계통의 고정도는 46.3-100% 사이에 분포하였다 (도 3).
여교배집단 및 육성계통에서 효용성이 검정된 마커는 총 341개로 염색체 별 24-36개 마커가 분포하였으며 (표 2), 마커 간 평균 거리가 1.1 Mbp 이었다. 다음 물리적 지도 (도 4)에서 파란색으로 표시된 마커가 효용성이 검정된 마커이며, 회색 마커는 다형성을 보이지 않았거나, 증폭이 되지 않은 마커를 의미한다.
최종 선발한 341개 마커세트의 리스트는 상기한 바와 같다(표 1).
상기한 결과들을 요약하면 다음과 같다:
원예적 형질(과피색, 호피무늬, 과육색 등)의 다양성을 보이는 30개 수박 핵심계통을 선발하였다. 잎 DNA로 염기서열재분석(re-sequencing)을 수행하였고, 수박 표준유전체 서열을 기반으로 SNP를 탐색하였다.
탐색한 SNP 중 염기서열 깊이(depth), 다형성지수(PIC, polymorphism information content) 등의 기준을 충족하는 유의한 SNP 12,674개를 선발하였다. 대량 유전자형 분석 시스템인 Fluidigm® EP1TM에 적용 가능한 384개 SNP 프라이머(또는 탐침) 세트를 개발하였다.
개발한 마커세트를 1개의 여교배집단(BC1F1, 28개체)에 적용한 결과 133개 마커가 다형성을 보여 BC1F1 개체의 유전형 분석을 수행하였다.
동일 마커세트를 원예특작과학원 수박 육성계통 244개체에 적용하였으며, 동형접합형(homozygote) 및 이형접합형(heterozygote)을 모두 구분할 수 있는 마커는 341개 였다.
최종적으로 효용성이 검정된 341개의 마커세트는 육성계통 간 다형성을 보여 분자표지이용여교배(MAB, Marker-assisted Backcross)로 활용할 수 있다.
이제까지 본 발명에 대하여 그 바람직한 실시예들을 중심으로 살펴보았다. 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자는 본 발명이 본 발명의 본질적인 특성에서 벗어나지 않는 범위에서 변형된 형태로 구현될 수 있음을 이해할 수 있을 것이다. 그러므로 개시된 실시예들은 한정적인 관점이 아니라 설명적인 관점에서 고려되어야 한다. 본 발명의 범위는 전술한 설명이 아니라 특허청구범위에 나타나 있으며, 그와 동등한 범위 내에 있는 모든 차이점은 본 발명에 포함된 것으로 해석되어야 할 것이다.
<110> REPUBLIC OF KOREA(RURAL DEVELOPMENT ADMINISTRATION) <120> REPUBLIC OF KOREA(RURAL DEVELOPMENT ADMINISTRATION) <130> RDA-P210015 <160> 341 <170> KoPatentIn 3.0 <210> 1 <211> 121 <212> DNA <213> Citrullus lanatus <400> 1 aataaaaatg gaaggataac taagtaaaac gttgaaagtg ggagaagatt tagatttttt 60 waaaaaatac aattagtaaa gtggctgttg atatgggtaa tttaaaatta aaatataaaa 120 t 121 <210> 2 <211> 121 <212> DNA <213> Citrullus lanatus <400> 2 atattgaaga tatcaattaa ttcttaatat caatgtttct attcgattca caaatagaat 60 kacatattga ttaatattag attaaagttg tgggaagtga aatttcaatc aataatcaaa 120 t 121 <210> 3 <211> 121 <212> DNA <213> Citrullus lanatus <400> 3 tccttttcaa aaaatatgac tatcataaat aatagtagga gagattcgaa ccagttaact 60 ygtgtaacaa tatcacaatt tctattttgt tttatcaaat gaacatgtgg ctggtgtatt 120 t 121 <210> 4 <211> 121 <212> DNA <213> Citrullus lanatus <400> 4 ttatatcatg tttacatcaa taatattttg ttgcttatta ttttttttta ttttttataa 60 ytttttaacg atatgataaa aataacgagt aacttctaat gtcaatgttg aactcataaa 120 a 121 <210> 5 <211> 121 <212> DNA <213> Citrullus lanatus <400> 5 tttgaaaact tccattctag aaaagagttt aagaaaagaa aggaagaaaa aaaaaccatg 60 ytcttcacac aagttttttc actttaattt caatctttta aaacacacct ttgaattttg 120 a 121 <210> 6 <211> 121 <212> DNA <213> Citrullus lanatus <400> 6 ttctctcaaa agaattaaat ttagaatttt tgtgttgaca aacatatcca ccaccgacaa 60 wtatatacct acatacatac gtgtatgtat ataaatatat atatccacac acgtgtatat 120 a 121 <210> 7 <211> 121 <212> DNA <213> Citrullus lanatus <400> 7 gtgtcttaat gctcaacttc agtacagaga aaatatttga tacccgaatg gtgtagcctc 60 ractagagag aatcaaactc aagcaactca attgaactca aaatgaagct attgctcaaa 120 g 121 <210> 8 <211> 121 <212> DNA <213> Citrullus lanatus <400> 8 ctaagtcatt taaggtttgg aactagcaat tgaaaagatt ttagagtgtc ctcaggtttc 60 rgtattgttc ctccaatgat tgtagtacca agtacttctt gccgagctct aatactccta 120 g 121 <210> 9 <211> 121 <212> DNA <213> Citrullus lanatus <400> 9 atggttatca tattgagact gatatgataa atgagataga acatctttat atcatatctt 60 ytatctcaca tgaaaaacgt atattggaaa agttgcttgc tttatcttct ggattatatt 120 a 121 <210> 10 <211> 121 <212> DNA <213> Citrullus lanatus <400> 10 gttttggact tactacttgg tttatgctga agcctatgtc tatgcttatt gtatggacag 60 rattattgta ttggttggtt atggttactg cgacttatta ctatgcactt aacttactac 120 t 121 <210> 11 <211> 121 <212> DNA <213> Citrullus lanatus <400> 11 ttttttaaaa aataaaaaat agtttttttt gcattgtgat atttaggtta atattttggg 60 yccaattact atatatttgc aactacccaa aaactttagt tactactagc agggttgcaa 120 c 121 <210> 12 <211> 121 <212> DNA <213> Citrullus lanatus <400> 12 aatgtgcgga gtcaacttaa tgccattaac agaaagcaaa gtggaaggta tgaatgttca 60 maataggtac agttgtattt agccaatttg atgtttctaa ttgatcgtgc tttcatcagc 120 t 121 <210> 13 <211> 121 <212> DNA <213> Citrullus lanatus <400> 13 attgattgca ttttcttcaa caaaatgagc atctctcctc caacgaatta actgtctctt 60 yttcaacaaa ctgagcatct cttctttaac aaattgaacg tctcttcgcc atctacaaat 120 t 121 <210> 14 <211> 121 <212> DNA <213> Citrullus lanatus <400> 14 gcatagcttg attataggtg agattgtggc taagtgttga gcgacttatc ttagttcaag 60 rtaaaacact taagccaaag tgcaagcata gggggttgga cgcatactct atctaaggtg 120 c 121 <210> 15 <211> 121 <212> DNA <213> Citrullus lanatus <400> 15 atctaataga aactccctta atttaagaga ataaaaagca cttttcaagc gcttttcact 60 mtgtaatttt gatttttccc aaaacaagct taatagcaca aggcgatcat tagcttctgt 120 t 121 <210> 16 <211> 121 <212> DNA <213> Citrullus lanatus <400> 16 caattttaat gggttaaaaa attgattata acgtaaattt aatttggttt aaaatttggt 60 yagttctaat atttgattta aaattcaaat ttatcactat tttttctttt atcatttacg 120 t 121 <210> 17 <211> 121 <212> DNA <213> Citrullus lanatus <400> 17 cacattcaag ttatgcagaa gaaatagtgg gaaaagagtg tgaggtaggt agacggtacc 60 ragtaaatcc aattgattgt tataggtttc atgtgaagga tggtggatta gacggtatcg 120 t 121 <210> 18 <211> 121 <212> DNA <213> Citrullus lanatus <400> 18 gattatcgct tcaagtcaac aaagtctcaa catttatttt tctcaaattt ttgcgttcta 60 ycctggacta tctgggaacc ttattcaaaa tttacacttg ggttttgata agaaaatttg 120 a 121 <210> 19 <211> 121 <212> DNA <213> Citrullus lanatus <400> 19 gaaaaagaat tacattaact attgataaac tataccaagt tttaatagtt aagataatac 60 rtttctttaa agtataattc gaatggcaaa tattattgat ctctagttta tactatacac 120 g 121 <210> 20 <211> 121 <212> DNA <213> Citrullus lanatus <400> 20 agacattaca aacagcctaa ccacaacaag cccttggata ttccaaagaa gggggaacat 60 yaggcaaccg atcaacaaaa agataaaatg atggcatggt ttgcttgagc aactaatcat 120 a 121 <210> 21 <211> 121 <212> DNA <213> Citrullus lanatus <400> 21 agtattcgag ggcagttcga gcggtttttc caattacaat tgtaatagtt tagttgtttt 60 ragccattag atgctaaaac cggtccactt gagtgtgttt aattaattat gcatttagaa 120 t 121 <210> 22 <211> 121 <212> DNA <213> Citrullus lanatus <400> 22 gtaacaattt agtctttata ctttcaattt tgtaataatt tagtcctaaa ctttgtcgcc 60 yttatatttt aaaatttgta atgatttagt ctctattata gaaattagtg ttaaggttta 120 a 121 <210> 23 <211> 121 <212> DNA <213> Citrullus lanatus <400> 23 ttaaactaca ttttatcaaa tcgtaataaa attttattga tggattaatt gtagtaagtt 60 rggattacaa atgtaattga attgtttttg atcatgttta cttagaatta caaatttgtt 120 a 121 <210> 24 <211> 121 <212> DNA <213> Citrullus lanatus <400> 24 atatgacaaa attaaagcat aaaacattaa caagtttttg ccaacaaaaa taatgtcctg 60 racaaatatt tgaatttcag aaagggagta atcttaacct ttaatgagtt tgatggatga 120 c 121 <210> 25 <211> 121 <212> DNA <213> Citrullus lanatus <400> 25 aaagatgcaa aaacaatccc aaagctagct taatgctcag tttaatttta aaatttatgt 60 staatgaata tagatttaat cagaatttat tttaccaata aatcaatgca aaactaaaat 120 g 121 <210> 26 <211> 121 <212> DNA <213> Citrullus lanatus <400> 26 gcaggtggac caaatagtaa agaggcaaaa gattaagaac ttaataaaat cactaacctt 60 yttaagaaag tgactagagg atggttgtct ttatggtgaa actatccgat gcaatgaagc 120 a 121 <210> 27 <211> 121 <212> DNA <213> Citrullus lanatus <400> 27 tatcataatg cacctagttg tttgaaggtt cattttcttg cctttgttgc ccacctaact 60 rtctcttgat ttcggtcaat tgatcttgaa aactcaaaag ttttgacttt aacatgacat 120 t 121 <210> 28 <211> 121 <212> DNA <213> Citrullus lanatus <400> 28 aattgtaaat aaagtattta ataagtccca aagtttcaat ctaagattag tgaataggat 60 yttcaacttt taattttgtg tctaataggt attcataccc ttaaaaaaaa tagatctatt 120 c 121 <210> 29 <211> 121 <212> DNA <213> Citrullus lanatus <400> 29 ctaaacaaat aaatagggga aatagaaaaa gactagaaga taagaaaaaa ggaaaaccca 60 yccattattg atgaaagccg ctaccacaaa atttgatttc attattttcc ggaatatgtt 120 g 121 <210> 30 <211> 121 <212> DNA <213> Citrullus lanatus <400> 30 tattatttat attacattta tattagtgat attttgttgc ttatttttta tgtttcttaa 60 rtttttctac gatataataa aaatatcgat tcaactctca aatacacatg aaaatatctt 120 t 121 <210> 31 <211> 121 <212> DNA <213> Citrullus lanatus <400> 31 aattaggcgt cgagtttgta aacaatttga tttcaccgca tactttgcca atcatgcatg 60 ygtccaacgc ataatcgaat tatgcgttca tcgataaacg atttctgtgt ttatcaagtt 120 g 121 <210> 32 <211> 121 <212> DNA <213> Citrullus lanatus <400> 32 aattcttatg atatcaagct atttatttaa aagagcaaaa gtttagtaga aataaattct 60 yatgatacca agctatttat ttaaaaaagc aaatttcaca cgacatgttt ggtactgatt 120 t 121 <210> 33 <211> 121 <212> DNA <213> Citrullus lanatus <400> 33 tgaaagtata ccgatgttaa tatacataca tatctcttct attatattat gatgtatcat 60 rtttatagta tcatacttca aatgaacatc aacttcgtta tgtaactacc taactccatt 120 a 121 <210> 34 <211> 121 <212> DNA <213> Citrullus lanatus <400> 34 tcaaagggaa tgcaaaagac gtgtccaatt tgcaacaaga aaatctgaac tgtggtcttc 60 ragaggttgg taatctttac accaacaaaa aagcaagaaa aatagaaaaa tcaaggatat 120 c 121 <210> 35 <211> 121 <212> DNA <213> Citrullus lanatus <400> 35 tgaattaata gaaattatta aaagatctat ggacaatgta aatattctct ctcattaatt 60 wgtagaattg atttaatgca tgcaccagca tctaagaact tgcttaagag tggagaaaag 120 g 121 <210> 36 <211> 121 <212> DNA <213> Citrullus lanatus <400> 36 cacccattat aggaagacat actagtggcg aatttctctt catttctttg ttgtccatga 60 mcaagacggc agttgattaa ctgagctgga aaagagtcaa aaatattaaa aaagaagaga 120 a 121 <210> 37 <211> 121 <212> DNA <213> Citrullus lanatus <400> 37 gtagaaattt tgattttttt tagtactgac aattttttct actctaaatt ttttgtggtc 60 yactaagtac acagtaatga aaattatttt aaatgacaaa aaatttttaa aaatatttac 120 g 121 <210> 38 <211> 121 <212> DNA <213> Citrullus lanatus <400> 38 ggacaaaagc gcaaagaaaa caaccctttt ttaaaacaac aaatatacat gcaatccatt 60 yaaactcaac taacatatag atatgtcagg gatcaagaag tttatggttc taatctctct 120 a 121 <210> 39 <211> 121 <212> DNA <213> Citrullus lanatus <400> 39 ggattattta tgtaaccaat ttttccatct ttttcaaatt ttttcaactt tttttttttt 60 wtaaataaat aaataaattt ttgggtacag atcaaggcat caaagagatg aatgcagggt 120 t 121 <210> 40 <211> 121 <212> DNA <213> Citrullus lanatus <400> 40 aaacaaaata tttcattgag taaatgaaaa gagactaaag ctagagatac aaattccgta 60 mggaaatgaa aagaaaaaga acaaaaaata attcagtcca caaatctcaa aaattaagaa 120 g 121 <210> 41 <211> 121 <212> DNA <213> Citrullus lanatus <400> 41 atccaacaca aaattaagaa ataaatgatc caatttgaca catcacagca gtatatatct 60 yccactaata gatatgaagt ttaaattcat aatagtaaat gttgaatgaa cttaaaaaat 120 t 121 <210> 42 <211> 121 <212> DNA <213> Citrullus lanatus <400> 42 catatatatc ataagaatga gagaccaaag gtttactttt tcttccaaat gaatgcaaca 60 wtttcaacca ttatttttca tgcaagaatt agaaagtaaa gggtcagaga ctcagactgt 120 a 121 <210> 43 <211> 121 <212> DNA <213> Citrullus lanatus <400> 43 catgctcaaa tagcattatc agaccatata catttcattt ccgaaaaacc atgaaaaata 60 ygatacattt caaaacattt tccttagttg aggcggagcg gagctcaacg ctactcaacg 120 t 121 <210> 44 <211> 121 <212> DNA <213> Citrullus lanatus <400> 44 tgaaaattga tacatccttt aaaacaaaaa atgaattaaa tataaagtaa aaataaaggt 60 kacattagaa gataacaata ttatgatcaa ttccctataa actagaccta cataattaca 120 a 121 <210> 45 <211> 121 <212> DNA <213> Citrullus lanatus <400> 45 gtttactact ttatatagac tttgttgttg attaatatat ttattttcaa gtatgccaca 60 yatattcatg cgaatcttaa agttttaaat gtaatttatg ttgatgtaaa taaatttatt 120 t 121 <210> 46 <211> 121 <212> DNA <213> Citrullus lanatus <400> 46 ctcaatggcc ctagaggggt tagtttataa ttggactata aatagtttgt tcattagagg 60 kttcagtggt acttaggagt taaatataac tacaggggta aaacggtgat ttgacccagc 120 t 121 <210> 47 <211> 121 <212> DNA <213> Citrullus lanatus <400> 47 acaaaagaag actattaaac acaatgaact aacaagttca atattttatt tacaccttca 60 wtgcccgaga tttttgtttt tatttttttg atatatgatt agctaagttg gttgttggag 120 t 121 <210> 48 <211> 121 <212> DNA <213> Citrullus lanatus <400> 48 ttaatgggaa cataatcaca ccatagtttg ctacaaattt ggataataac caattaatcc 60 raggaagcct agcatttgct taattgttgt cagtattgac ccattattca ttgcctccaa 120 c 121 <210> 49 <211> 121 <212> DNA <213> Citrullus lanatus <400> 49 tcaaatgtgg gatgtaagat tcctggcaag aaatcatata aatttggtga cgcatatttt 60 ygcattggcc tatttctatc atgtgctaac agaataggat tagtttgagg gtgtttgttt 120 g 121 <210> 50 <211> 121 <212> DNA <213> Citrullus lanatus <400> 50 tcaaactaaa cttgatttca ttatgtacaa cgataacgcc ttcaacaacg atccaaattc 60 rcattaagca tagcagggtt taattgtgac gaatcaaagg tcagaaagcc ttgtctaaca 120 t 121 <210> 51 <211> 121 <212> DNA <213> Citrullus lanatus <400> 51 ttctgattgt caattctgat tcatgcatag gaaatgcaac tcttctactc acaagcttgc 60 rtgtacttgt agagattact ctagtgttac ctttttcatg gatgcctaat atttttaatt 120 a 121 <210> 52 <211> 121 <212> DNA <213> Citrullus lanatus <400> 52 tgtattagaa gttgattata caaatactat gttaattgag ctatgtttgt taaaagaaaa 60 kttgaaaaat gagattaaaa attgtaggag tatattaaat agtgtagtgg aataggaaag 120 t 121 <210> 53 <211> 121 <212> DNA <213> Citrullus lanatus <400> 53 attgtttttt gggaatttgc aacaggtggc aaatccaaaa tgtataattg ggggcgtgct 60 wtcaacgtat ggaaaattgt tgaaatgaca aaattctcaa atttaaattt ttgtttattc 120 c 121 <210> 54 <211> 121 <212> DNA <213> Citrullus lanatus <400> 54 gtgagacatt ttgaacaatc aaactactaa tcaacaatgg tcggaagtaa atgacagccg 60 rccttaattt tagaattttt attgtggttt ttatagattt agctttgtat atataagtcg 120 c 121 <210> 55 <211> 121 <212> DNA <213> Citrullus lanatus <400> 55 ccgaaaaaac accgctaaaa aagtatttac aacacgtaaa ggcacgtcgc tgtatacgtc 60 rtagtagctg catagtcata agttttagtg acaaattcct agcacgctgc taaaacttaa 120 a 121 <210> 56 <211> 121 <212> DNA <213> Citrullus lanatus <400> 56 gggcgttgga cttctccagt agccagtgat cgtttgttac attagtggct aatgtgaacg 60 rtatctacct taacatgttt ttctgctgaa ttcagatgct tatagaaaat gtttgactgt 120 g 121 <210> 57 <211> 121 <212> DNA <213> Citrullus lanatus <400> 57 aaaaatagaa ggcaattata ataggtatga gttcattttt caattgggta taagaaaatt 60 raggaccaat tatgtgattt agctaattat aataaccaat gaaactaagt ccaaattata 120 t 121 <210> 58 <211> 121 <212> DNA <213> Citrullus lanatus <400> 58 agactatgat ctcaccattg tggtatatga tcaattttaa agtatgattg tccaatatat 60 yagtgtttac attcattttc ttcgggtaaa tattttgtca ataacaaggt agattgattg 120 t 121 <210> 59 <211> 121 <212> DNA <213> Citrullus lanatus <400> 59 tcgtatttta cttacatttg ttgtactaaa aacaaaaaca agaacatttc ctttatttta 60 ktttatcttc gatttgatcc gattattgtt taaaaagatt caagttggtt attatgtttt 120 a 121 <210> 60 <211> 121 <212> DNA <213> Citrullus lanatus <400> 60 atggtttgta gtataagtat aacgttgggt gccttatatc ctggtgacac tatgaatatg 60 rtccactttg taattgttac aaaagttgta aagtgttaca aacgatttga tcctaatcat 120 t 121 <210> 61 <211> 121 <212> DNA <213> Citrullus lanatus <400> 61 tgatttgact tagataaata ttagtgataa tcacttataa accccttatt aatagacttc 60 yatcacttat agctttaaaa tgttagtctt tgaaagttga tattttactt tcacattagt 120 c 121 <210> 62 <211> 121 <212> DNA <213> Citrullus lanatus <400> 62 tattaggggt gagtattgat agaccgaaaa tcgagccgac cgaccaaaat caactaaatc 60 kaagtcaatc ggtcgaagaa gaggggggac ttagttggtg tcagtttgga aaaatttcaa 120 a 121 <210> 63 <211> 121 <212> DNA <213> Citrullus lanatus <400> 63 tgataatccg tgtctaaacc attctttcaa agtattaaat cctattgatg tatttcatta 60 rtaacatccc taacatagtt tagtgaataa atcacaaact aacacttcaa atttagattt 120 a 121 <210> 64 <211> 121 <212> DNA <213> Citrullus lanatus <400> 64 ttatttcaag ttgttatctt ggcgtatgta acttttgtat tgtgttggct tagggcaatt 60 ytacttttta attattgttc ttttatctaa aaaagaagtg atttgacatt catatgagag 120 g 121 <210> 65 <211> 121 <212> DNA <213> Citrullus lanatus <400> 65 aattaggttt atagtcctga tttattattt ttacaaatgg cagaaaaaca agttcagccc 60 rcgtgataca cctgatatac atgacacaca atacacagtt gatacacact ttgatatact 120 t 121 <210> 66 <211> 121 <212> DNA <213> Citrullus lanatus <400> 66 gaaaatttct ctaagaggaa gcaaagtgcc aaatgggcaa atgattgctt tgtgcttgtc 60 rctgccaatc cctcattcca tttgttcttt aggaacattt ttaactaaca aagacgaatc 120 a 121 <210> 67 <211> 121 <212> DNA <213> Citrullus lanatus <400> 67 tactttaaat taactttata cacaaacgat aaatattcca attttgcgtt tccctcaata 60 kattattatt ttttaaattt taatattaca tcttactctc ttcataacca tttcgcattt 120 a 121 <210> 68 <211> 121 <212> DNA <213> Citrullus lanatus <400> 68 tgttacattc tcaataattt aattaaaaac ttatgacaaa aagttacaac tacaaaaatt 60 rtaaataatt ataccctaca tccacttaat atcacatttc ccaccaagtt aaataatctt 120 g 121 <210> 69 <211> 121 <212> DNA <213> Citrullus lanatus <400> 69 taagtcttaa ctttcaattt tgtgtttgat aggacaatat tttctcaatt gatttgtgaa 60 mttccaaatt tataacaaat tgtttttcac atatttaaat ttttaaaaat taagtatttt 120 t 121 <210> 70 <211> 121 <212> DNA <213> Citrullus lanatus <400> 70 aatatatata tatacatcaa agccaaataa aaaaagtgat agatgtctat ctggatctat 60 ygcggtctat cacagataga aagtaaaatt tcgttatatt tgtaattatt ttcaacagtt 120 t 121 <210> 71 <211> 121 <212> DNA <213> Citrullus lanatus <400> 71 aaagaaaaat gaaaaagttc attgtgtata aacaaagtaa cattttgctc attctcattg 60 yacagttgac ttttattgag ttcatttttc attagtagtt tagattgatc acccatatta 120 g 121 <210> 72 <211> 121 <212> DNA <213> Citrullus lanatus <400> 72 atagtggtga agatattttg tgttggatca atggtgtggg ttttgttgga aaataaaaaa 60 kaagtaaagg gaaatgtaag ggatgggaag aagctaaggc aattattgag aaatgagttg 120 g 121 <210> 73 <211> 121 <212> DNA <213> Citrullus lanatus <400> 73 tatgttagtc aaatcaatga aaaatatatg attctcatca atttgttccc cacttcgatc 60 yaaataggtg taataaaaga taaaattaaa attattaaaa gatgcgagga aaaaactgga 120 g 121 <210> 74 <211> 121 <212> DNA <213> Citrullus lanatus <400> 74 ttagtgtctt ctcataacca aaagggttgc caatggcata gcagctctga ccaacacgta 60 satttcgaga ggcaccaaag actatgggct tcagttcttg tccttcaagt tctacctgga 120 t 121 <210> 75 <211> 121 <212> DNA <213> Citrullus lanatus <400> 75 aaggagatga aaaattaagg ttgtatcaag agtatattaa tgttcattat tgttgaaggg 60 maaattacaa ataacagaag aaaatagagt aaatattgag aaacaaacac ataagcaatt 120 a 121 <210> 76 <211> 121 <212> DNA <213> Citrullus lanatus <400> 76 tcttttatac ttacataaat tatatattga aatggacgct cgtcatattt aagatcaagt 60 rttttggcat cgatttgcta ctcgtcattt ttactaagag attctattgt ctaattgtct 120 a 121 <210> 77 <211> 121 <212> DNA <213> Citrullus lanatus <400> 77 ggtaaaatat gacaatagaa tcagaattat tcaaatttaa tactttgtat taattggttc 60 mtatttccaa tttaacatat gtgaagaaac caattaaagg actgtaaagc gggaaggagt 120 t 121 <210> 78 <211> 121 <212> DNA <213> Citrullus lanatus <400> 78 atctatagct ttcattcata atttgacatg tcatcaaatg atatattaat ttatcattca 60 yttatttttt ggatcccacg tagttcttga atactacttt attatgtgac aaacaatgaa 120 t 121 <210> 79 <211> 121 <212> DNA <213> Citrullus lanatus <400> 79 tgaaggcctt agaaaagcaa atatatgttt gcgtagagaa acaaaattta aaattgataa 60 yactttgttc tttagtaagt caaagagaaa tttaataagt tttaaagata tatgttgata 120 t 121 <210> 80 <211> 121 <212> DNA <213> Citrullus lanatus <400> 80 taataatact gctaatgaac tttattgata taccattgat atactttata atgatatact 60 matttatact gttaatacac ttaactaaca tattgtaaat atactttata atgatacaca 120 t 121 <210> 81 <211> 121 <212> DNA <213> Citrullus lanatus <400> 81 tcgttctttg gatccccggc aatggcgcca aaaatttgat gcgcactttc cgtgtttacc 60 rcaagtatac gagtcaagtt ataatataag acgattaagt tccgaatatc gttccactga 120 g 121 <210> 82 <211> 121 <212> DNA <213> Citrullus lanatus <400> 82 tcactccatt aaagacctac aactgcacta ttcacactat agatatattt ctatgtccat 60 wgaaataatc tatcaatagt aagttgaccc ttcacaaatt actcgtaact acaattgggt 120 c 121 <210> 83 <211> 121 <212> DNA <213> Citrullus lanatus <400> 83 gagtgttacc tattctacaa tctgttaaca ctgttatata tcataaactg ctggcaattg 60 rcttagcttt tacaatagtt atgcgataat ttactatagt tatgcaataa tttactgata 120 g 121 <210> 84 <211> 121 <212> DNA <213> Citrullus lanatus <400> 84 agtagtatag ctcaaaagtt cttaccagat ctgaaagttg ttccaattat gcatcttcct 60 rtcactcttt attgtgataa tagtggtaca gttgcaaatt ccaaagaacc tagaagccat 120 a 121 <210> 85 <211> 121 <212> DNA <213> Citrullus lanatus <400> 85 ctagacttat acaaactcat tatataagat attgatctca ctcacatgtc tctacatgaa 60 ygatttggat ataatcattt gcaacagtta caaagtgagt cgtatcaata atgttattaa 120 g 121 <210> 86 <211> 121 <212> DNA <213> Citrullus lanatus <400> 86 ccgaagtttt tccatttcct cgaagcttca atccaatctt ctatctgcct tgtatcacat 60 ytaatcagtg ctttgtctac gaatagtggg ttaattatga attttacatt gaataaatct 120 t 121 <210> 87 <211> 121 <212> DNA <213> Citrullus lanatus <400> 87 tcgacactgt agacaaaagt agcaaaacac agtagcatca caacattgtc ctatagcact 60 rcaccgctat gacttttcca gcacgcatcc cacacatttt gtttttggac aacggtccga 120 t 121 <210> 88 <211> 121 <212> DNA <213> Citrullus lanatus <400> 88 atctttcttt tttgttctct ctatgcatta ttttccaagt ctgttcaatc ttcaaagcat 60 ytcaagatgt tggtaaattc aagttggaaa tgattcacct caactttgaa ggatttgaat 120 a 121 <210> 89 <211> 121 <212> DNA <213> Citrullus lanatus <400> 89 taggtgtttg atgaaagcct ttagcaacaa gtcttgcttt ataacgagca atagaaccat 60 ytgaattcct tttaatttta aacacccatt tacaaccaac aattttctga tcagtagttt 120 t 121 <210> 90 <211> 121 <212> DNA <213> Citrullus lanatus <400> 90 aatcttatat ttgatgggat taatggtcga aaacaacaaa ataagtttga gataaattac 60 kaacgaaatt tgaaggaagc ggaaggatta aagtcgtaaa attgttgact ctcaagacat 120 a 121 <210> 91 <211> 121 <212> DNA <213> Citrullus lanatus <400> 91 tggaccaatg aaatctaggt aaccaataaa agaagtttat atggagatag acacgaaagt 60 waatttaaaa ccataaacaa taattgataa acaagggcaa acaataattc aaccttcgag 120 t 121 <210> 92 <211> 121 <212> DNA <213> Citrullus lanatus <400> 92 tttaatgtta tttcttggtt gggattgttc tgatttagat ttagttatgt gataaaaaaa 60 wtttttgttt caacatttta taaatcccca agttttcatt cttagagtag gtaaagccat 120 t 121 <210> 93 <211> 121 <212> DNA <213> Citrullus lanatus <400> 93 attcggatta ggtttatcgt tggatgaatt gcattgctga tattgaccct aaaaaagaaa 60 yggtaggtac agctggttcg tgaacaacca accatcgcac tgtaaaaata ggataagttt 120 t 121 <210> 94 <211> 121 <212> DNA <213> Citrullus lanatus <400> 94 ccatttaatt agatgaacac aacacaacca attggctaat aacattaaga atgaggaata 60 wgcttagttg aatgcccaac tggaaacgtc aaatcactat tcaatataat tcttcctacg 120 t 121 <210> 95 <211> 121 <212> DNA <213> Citrullus lanatus <400> 95 tttcgtgaat acccattggc gttggtttta attgttggac ccattgttta aagtatggaa 60 ratgactgtg acagttgcac tttggatccg ttttcgaagg ggctttggag aaggttgaga 120 t 121 <210> 96 <211> 121 <212> DNA <213> Citrullus lanatus <400> 96 tttctttcat ttcaaagacc atggacatca tttgaaaata attgaaagtt acttcaaagt 60 saggagatat cttggttaat taaaccaaaa ttatttgata atccatctaa aattaggcac 120 c 121 <210> 97 <211> 121 <212> DNA <213> Citrullus lanatus <400> 97 aacttttaac aaaattgcaa ggtgacttgt agtttttgga tgactagact actaaataag 60 ktagctatgc aaaaaaatct aaagattcaa ttttgaataa gatggcaaag ctggaatcta 120 a 121 <210> 98 <211> 121 <212> DNA <213> Citrullus lanatus <400> 98 tttatggagt taacttataa tttaagggct ctttggccca tatttggggc tccaaatgta 60 rtaataatat tataactatt tataatctac cattatttgc atagtaatat ttgaaatctt 120 a 121 <210> 99 <211> 121 <212> DNA <213> Citrullus lanatus <400> 99 tggttaagcc atgatgattg gtttacaata atgccaatta actaatcgat atctctttat 60 ycctaagcca cttatgacaa ctatgtgaca ttttcggtaa tgtcgtgaaa agtcacgtag 120 c 121 <210> 100 <211> 121 <212> DNA <213> Citrullus lanatus <400> 100 agaaattatt ttagagtggt agcatgaagg aaaggctaaa gttctattgc gtagcacttc 60 yagagctatt ttgtggatga tttggatcat tgactgatag aaaacttaga gaaaagattc 120 a 121 <210> 101 <211> 121 <212> DNA <213> Citrullus lanatus <400> 101 tattagtaat atttatttca attttagttt taaaaaataa aaattttcac acataaaacg 60 yctttttatt taatttttat ttacttaata aggattcgca actgaacttc aatatatatt 120 a 121 <210> 102 <211> 121 <212> DNA <213> Citrullus lanatus <400> 102 attaaatagg ggaagttgca aacatatcaa tcaaactcaa aatattagta gatataacac 60 ratgaaaaaa tatttgtagt tatggaaaaa tttaaatcta actttcagag tctatccatg 120 a 121 <210> 103 <211> 121 <212> DNA <213> Citrullus lanatus <400> 103 ttatcacctg tcaagccact tgctagtcat ttcttccaag aatgttaaga ataaaagtag 60 rgcttacagc gacactagcc tcattttgga aaaaaaatct acacttttgg aaagtttttc 120 c 121 <210> 104 <211> 121 <212> DNA <213> Citrullus lanatus <400> 104 aacacaagaa ttaatgtgga aaaactccta actggagaaa aaaccacaaa ttagaaaaat 60 wccggtgtgt gaaaaaatgt tacaatcacc caaaataatt tcctccatgg ttctaattac 120 a 121 <210> 105 <211> 121 <212> DNA <213> Citrullus lanatus <400> 105 acgaggagga gttaagccac gaaaatttaa ggtaagctag aaaacatatt tctctacaag 60 kttgtagaag gaaaaaagtt ccaaattcgg ctggaaatat gacttttaac agatagaatt 120 g 121 <210> 106 <211> 121 <212> DNA <213> Citrullus lanatus <400> 106 gaatgcatac taataggcct taggttaatt caacttaggt tctaattcag ttcccagtta 60 rgaggtgttc cgtaaaccgt caacttaaat accacaatcg tgacaaaact gaagtggtag 120 a 121 <210> 107 <211> 121 <212> DNA <213> Citrullus lanatus <400> 107 ctttgggtga agttatttga attaagtttt aaataatttg tggttagatt taggcctagt 60 ratttaattt aagttttggt ttatgttcta ggttgtaatt caaaggtttt ggtggagttt 120 g 121 <210> 108 <211> 121 <212> DNA <213> Citrullus lanatus <400> 108 atgtatttca tatctaataa ttaaaactat tctaatattg tcacccccac cttagattat 60 ycttttgaac ctaggacgaa attttgacga tagtggaatc tactatcaaa acttttctta 120 t 121 <210> 109 <211> 121 <212> DNA <213> Citrullus lanatus <400> 109 ttacaggggt aaaacgaaca tttgacccag ctgtaattat gaacaactcg tgtaggattg 60 rcttccttat attgattata tccttgaata caacttgtta tatagtgcac aagagttcaa 120 t 121 <210> 110 <211> 121 <212> DNA <213> Citrullus lanatus <400> 110 agttatggac tcctgtctac attcctttga tttgtatggg tgagagtcgc tcatatcgtc 60 ractcaataa gcctaccatc ttgcgaactt attcaagttg ggagcttgaa acttagctac 120 a 121 <210> 111 <211> 121 <212> DNA <213> Citrullus lanatus <400> 111 ctcctcctaa cacactctgc tatgtgttta ccatacacct tatgcatcca agcttttgac 60 ktctcccaag atgtgtgttt taagcttggt tctagtccat taatctaact cccaactatg 120 c 121 <210> 112 <211> 121 <212> DNA <213> Citrullus lanatus <400> 112 ttgatattct ttgccaggac tcttgcaaga ggtttagtat gatataagag gtttagtatc 60 rtccttaggg atcaaatatt atggattaat ttatctactt tagaattatg tcaattttat 120 t 121 <210> 113 <211> 121 <212> DNA <213> Citrullus lanatus <400> 113 cagtatacgt ttttcatgtg agacagtaga tataaagata ctttatatta ttcttaccat 60 yagtctcaat atgataacca ttgcaactta tatatttaaa actcattaaa tttctctttg 120 a 121 <210> 114 <211> 121 <212> DNA <213> Citrullus lanatus <400> 114 aaatgagggt aaaacctatt gattgaagat ggatctataa aagaaaacaa gaccaagccg 60 rtaaggtaca gatctttaag gctcaactag tggcgaaggg ttttattcag agagagcggg 120 t 121 <210> 115 <211> 121 <212> DNA <213> Citrullus lanatus <400> 115 atagagattg acccataggg tcagccataa agccataagt ttatgggttg gttgcacaaa 60 yaacaatcag gctcaaacta ataaaagatg tagcacatga aaaaatatat tagggatata 120 a 121 <210> 116 <211> 121 <212> DNA <213> Citrullus lanatus <400> 116 tggaggatgc aattatatta agatatttaa ttggtcttat atgtgtatga aaatctggac 60 rtgcatctta tggatgtagt cacaagatat ttatatggac tcttaataat gacatttata 120 t 121 <210> 117 <211> 121 <212> DNA <213> Citrullus lanatus <400> 117 cacgagcccg agtcgaggtt aaggtccaac tagaacttca agagatgagg agatgattga 60 raaaaatcta tgaataatcc taataaaaga aagtataagc aagctttggt tgagaccaat 120 a 121 <210> 118 <211> 121 <212> DNA <213> Citrullus lanatus <400> 118 aggcctaaca aaagaaaatt ggactaaaga actattagtt gctagtttgt tgggtattct 60 rattgctctc aaggtttcaa gtttatgatc ccacttctaa ggatgagttt gtgggggaag 120 a 121 <210> 119 <211> 121 <212> DNA <213> Citrullus lanatus <400> 119 cctttgattt gtatgggtga gattagtcgt ttttcaactc aatatgcgta gcatcttggg 60 racaagatca agtgaagagc tagggacata attatataag atggaattca ctcattccca 120 t 121 <210> 120 <211> 121 <212> DNA <213> Citrullus lanatus <400> 120 gaatatttag tttacttaac atataataat gtacgaatat ttttttcaaa ttttatggat 60 rtatagatat atgaagcaat tttattttca tcatagaaat taaatcattt ataactatct 120 t 121 <210> 121 <211> 121 <212> DNA <213> Citrullus lanatus <400> 121 aacaaatgtc agagtgtcaa atgtccaaca cctatcggac acagacatgc tagccaatct 60 raagtatttg tgcttcttag ctcaactgag tgcaatttgt agattctacc aagccttttt 120 c 121 <210> 122 <211> 121 <212> DNA <213> Citrullus lanatus <400> 122 aaaagaagga gagaaatgtc aaacccatta gatgcaaaca atattgattt tttgtaggcg 60 ragctagatc aacaactctg tcgcgagaca tgaagatcct atgttggaat gcccatggat 120 t 121 <210> 123 <211> 121 <212> DNA <213> Citrullus lanatus <400> 123 tcaatgaatt aaaccttgaa ttgacaaaga gtgttgttct ttaggaatca aaaccatgta 60 ragattgagt aagttccttg agagaagaat tatctatcac aaatatatat acataggcat 120 g 121 <210> 124 <211> 121 <212> DNA <213> Citrullus lanatus <400> 124 ttaaaaaaag ggaaaaatat gatcaattta tatccacaaa ctttaaggtt gtattaacta 60 rttaaaattc taaactaata gttgtaccaa tttaaaattt gaaatttcat aactacatca 120 a 121 <210> 125 <211> 121 <212> DNA <213> Citrullus lanatus <400> 125 taatcacaac taagttgctt tctttcctgt acttgtgaat tgaatcccat agaaaacaca 60 ratttctatt tcataattat attctatatt actttgacat gttcattcta caaaagttca 120 g 121 <210> 126 <211> 121 <212> DNA <213> Citrullus lanatus <400> 126 gtactgatat tgtcactctt aagaggagcc taagtactct aagagggagt ctcacatcta 60 kgaagagctt aggtggttag cttgttaagc tctacaagag ttgatgtaga agttggtgtt 120 t 121 <210> 127 <211> 121 <212> DNA <213> Citrullus lanatus <400> 127 aagttattgt ttatagtgtt tgtattatca agtttgaggt taaaggttta attttttatc 60 yacatgtcgt aaaagaaaaa cagggtacat gtcgaattcc ctagatgaaa tgcacaatta 120 a 121 <210> 128 <211> 121 <212> DNA <213> Citrullus lanatus <400> 128 atttatagtt attgttatgt agaggtcaaa tttttttccc tattctaatt aaggcatcaa 60 yatgacatga atttttttag ttgccataaa catacactta aaatcaacat actaaacaaa 120 g 121 <210> 129 <211> 121 <212> DNA <213> Citrullus lanatus <400> 129 ggattccatg tggaaaatcc ccttccaggt actcgtcgct agccatgata ttatcttcat 60 yttcaacttc actactctga ccatatgcct cggtacatat accgtaatct tcttcctacg 120 a 121 <210> 130 <211> 121 <212> DNA <213> Citrullus lanatus <400> 130 gagtaagaga catgcatctc aacttaattg tttgcgtgtt ttctttttca atatttactt 60 yagtttcctt tattatttgt aattccctcg gctatatata ttaattaggg gtgttatatt 120 t 121 <210> 131 <211> 121 <212> DNA <213> Citrullus lanatus <400> 131 atcaactatt tatttattat tttggtttaa ttactagttc gggtttattt gacctttgaa 60 rttagaaaag aaaaatagct aaatagtctt tcaactttca attttgtgtt taattagttc 120 t 121 <210> 132 <211> 121 <212> DNA <213> Citrullus lanatus <400> 132 tgaagataaa acgttctcgt taatcctgaa tagcctcatt aacaatgaga actaactcct 60 ygtcataaat gcccctgggc atctaatatt gtcgaagggc gaactttttt caatctatta 120 c 121 <210> 133 <211> 121 <212> DNA <213> Citrullus lanatus <400> 133 ccaaaaaatt aagatccaat ccaaaaatac accataaact aacctatgta aagttactaa 60 rtttgaccca ttaatgaaac taaacttaat tacattgttt aatgtacatt tttatgcata 120 t 121 <210> 134 <211> 121 <212> DNA <213> Citrullus lanatus <400> 134 actatagacc ttgtggttag tacgatcata tgtcagttga gctatactcg ttttaaccta 60 yaatgtactt tcttgcatga tattatcatt atttttaaaa ccaattgaga tcaaaattag 120 t 121 <210> 135 <211> 121 <212> DNA <213> Citrullus lanatus <400> 135 ggaatattat ttggattaaa cttatgatta agttgtgatg catatttctg aatgtatatt 60 rgaatgacat taattgttat cctatctata gggtcatata ctctctattg attgtgaacc 120 t 121 <210> 136 <211> 121 <212> DNA <213> Citrullus lanatus <400> 136 tcccaaattt aagaggataa aaagtctttt ttgagtgctt ttcaaatctc gtttggaagc 60 rtgtgattgc tttacttcaa ttacgttcaa caattaaaac acaaaattat aacatacaat 120 a 121 <210> 137 <211> 121 <212> DNA <213> Citrullus lanatus <400> 137 ataataataa ataatgattt cttatgcgtt ggtaagttct cttccatctt gccctcaaat 60 waaaaaaagc ttctctccat tgatgataac atagcttaaa gttaagccct aattgatgaa 120 c 121 <210> 138 <211> 121 <212> DNA <213> Citrullus lanatus <400> 138 tatccctata ctatagacct tttagactgt atgttgaaca ttgatctctg tatatcccca 60 yatacggttc aaaactcata aaacagctta ggatgttagt ttattggatt tagggttatt 120 a 121 <210> 139 <211> 121 <212> DNA <213> Citrullus lanatus <400> 139 tttgtctacg aaaagtaccc gagtggatgg ttagggatcc tgcctggaag gtaagactat 60 rtgcacatag gggaaacata gggagactac agtgaacatt tatacacata tttataagtt 120 t 121 <210> 140 <211> 121 <212> DNA <213> Citrullus lanatus <400> 140 atagaaaaga agtcctgcag aaaggtaaga taaacattaa tcatgaaact tgattttttt 60 wattttttac agtcacaacc acttccatgg aacttgataa atcagtactt catttgatgt 120 a 121 <210> 141 <211> 121 <212> DNA <213> Citrullus lanatus <400> 141 ttggcgtagc acaaagcaag gttgcattgt tgactctacc gtggaggtag tattgaaaag 60 macctaaata atgctttgtt tgcctttgat ttagggatgt ttttattaga tatgatgtaa 120 t 121 <210> 142 <211> 121 <212> DNA <213> Citrullus lanatus <400> 142 acaattacaa atgggtcata tccgaagtgt tacaagaata aagcacccaa ccttattcct 60 kttaggtttt atgccctaaa actcatagat tgtaaatgta ataatttgac cgtaattaaa 120 t 121 <210> 143 <211> 121 <212> DNA <213> Citrullus lanatus <400> 143 catacacaag cccttagata cttgaggata ttcttaacgg caatccaatg tgcaaacccg 60 rgattggatt gatacctatt gacaattctt actgcaaaac atatgtttgg ttgtgtacat 120 a 121 <210> 144 <211> 121 <212> DNA <213> Citrullus lanatus <400> 144 acttggtttg tttggtctgt ctctatttga actttggatc ctcttctaat cttgtaatcg 60 ragttaattt cattgatgtt ttcatggtat atgattctaa aaactgatcc aactaaatcg 120 a 121 <210> 145 <211> 121 <212> DNA <213> Citrullus lanatus <400> 145 tttcgaccat attttatcga agatcattta aacctttgaa aaaagtatac atttatcaga 60 sgtttttaga aagccttcaa aaaatacatc cattttataa aagattataa aaaaccttta 120 a 121 <210> 146 <211> 121 <212> DNA <213> Citrullus lanatus <400> 146 ctttatacac ttcactagct atcaacacat caccaacagg agtgtaaaca gacaactcct 60 yaggtaatgg ttctattgta ctatttactc tttcagcaaa cactttagca ataaaagaat 120 g 121 <210> 147 <211> 121 <212> DNA <213> Citrullus lanatus <400> 147 atctcaagtt tttttttcac aattttagct aacaaaatat cttttacctt accaaaactt 60 ktgaagttaa accacacttg aagactgaag ttctttggaa tacatacatt cttccaaggc 120 t 121 <210> 148 <211> 121 <212> DNA <213> Citrullus lanatus <400> 148 gacaaacaaa catataatcc gtggaagtaa gtaagaattt tccaatgatt gaggatcaaa 60 kttctatttc tgagttatct gatgtctaga taaaaaagga aacttgtttg cttagaatcc 120 a 121 <210> 149 <211> 121 <212> DNA <213> Citrullus lanatus <400> 149 aaaagtggtt ggttctatta gttatagttg gatgtatcct attgaaagga gtttacagac 60 mctaagcaat acgtacgaaa caaagcacgt cctaagggtt ccattgtaga agcatttata 120 a 121 <210> 150 <211> 121 <212> DNA <213> Citrullus lanatus <400> 150 ccatggtgga tgcattatcc catactatct gtgatcggtg agtattttga agatatagct 60 raacagggtt agatgaacta agatccatat tcaacactgt taaaataaac caaatacatt 120 a 121 <210> 151 <211> 121 <212> DNA <213> Citrullus lanatus <400> 151 cacacagttg gcaacccagt tcggtgtaac cacacctacg tttgggaggc agagtgccta 60 ratgaaaaaa gtttcactaa tagagttaag cgattataga ggaatgctta tcttatggtt 120 a 121 <210> 152 <211> 121 <212> DNA <213> Citrullus lanatus <400> 152 aagtgtatca atagtagaaa cgagtgtaac attataaagt atattaacaa tatattaatc 60 wagtgcatca atagtataaa cgtgtgtatc attttaaact atatcaacaa tatatcaatc 120 a 121 <210> 153 <211> 121 <212> DNA <213> Citrullus lanatus <400> 153 tcaaactcac ctttgtcatc agtagttccc tctccttctc ttgcttccgc ttccccaact 60 ytgtctcatg ttcatcactc tagttcacag ccatctgaag cttttgctat cccatcagga 120 c 121 <210> 154 <211> 121 <212> DNA <213> Citrullus lanatus <400> 154 tccttgttga ccaagatact tgtttcttgg ttaaattatg aacttagttt ctaaacgtta 60 raacgttcat gtttgtgttt gtgtttagta ggtctagaaa atttaaaaat ctaagtaggc 120 a 121 <210> 155 <211> 121 <212> DNA <213> Citrullus lanatus <400> 155 tagacctcaa agccaatatt gtggaaaata gaccacaaag taacaagaaa atgaagtttt 60 ytggtgaagg ttcaagtcat agacaatgat ccacaaggaa attcaataac aaatgcttca 120 a 121 <210> 156 <211> 121 <212> DNA <213> Citrullus lanatus <400> 156 aaaatttggc aggaacgaaa ccaaagtaaa gagaaaaaat caaaatagat ggaaaataaa 60 raggaaatat gatttttttt tcttttttct gtcatccaac caaaattatt attctcaatt 120 t 121 <210> 157 <211> 121 <212> DNA <213> Citrullus lanatus <400> 157 tttatcgctt gagtgataga aatttatcac agtcgtcgct aaattggtaa acgtctattg 60 ygttctattg ctgttagaca ataagttttt cctatattgt atatagtttg actcattttg 120 c 121 <210> 158 <211> 121 <212> DNA <213> Citrullus lanatus <400> 158 gctaaatata tattaaagat ttgtacctta ttttttcaaa ggtcgtagcc ttcaaaaatt 60 rtcaactttc ttgtagtgta gatgtcacag agtaaacgag ttaatataaa tatgttatct 120 t 121 <210> 159 <211> 121 <212> DNA <213> Citrullus lanatus <400> 159 ttatggtaag aaagtcaccg aaaataaaca taattcaact aatataaact acgtgctcta 60 ratttctatg tcaaaggttt gaatcttttc ttatatgttg aacttaatgg aaaaaaaagg 120 t 121 <210> 160 <211> 121 <212> DNA <213> Citrullus lanatus <400> 160 tatttgtaaa tattttagtt catttttcta tatttgccaa caactcttat tttttatttt 60 raatatccat gaacatctaa aattagaaaa taatttttgg aaaattatct ctctctcctc 120 t 121 <210> 161 <211> 121 <212> DNA <213> Citrullus lanatus <400> 161 aaccaaaatg ttactaatag gaatattata taaaggaaaa aaattatcaa tttataccat 60 yaattatatt ttgtttgaaa aaaattgtgt aaaactaata attgtattag ttattcttct 120 a 121 <210> 162 <211> 121 <212> DNA <213> Citrullus lanatus <400> 162 aaaattagac aacaaatgaa gaaatttgga ggtagaagta atatttattg acttaatttt 60 maaaaaagaa atgattatca aacaatgtgt tagtttctca tcttgtaagt atagtcttta 120 t 121 <210> 163 <211> 121 <212> DNA <213> Citrullus lanatus <400> 163 tttgtatttt ttttaattta aaaaacatgg tttcaaatta tgatttattt tagaccatat 60 rctttcaaaa agaatatttt ggtttgctga cataaaaatg tctattttat aagtttctaa 120 a 121 <210> 164 <211> 121 <212> DNA <213> Citrullus lanatus <400> 164 acacgaaggg aaaacaataa gacttatata taagtgataa gcaccctaac tttatatcac 60 ytatagctta aagtgttaat attacaaagt tgatagtcat taatagactt ccatcactta 120 t 121 <210> 165 <211> 121 <212> DNA <213> Citrullus lanatus <400> 165 acatcggaga agtttgggag aatttaattt ctccctgctt ctcctaatgc cactttaaat 60 rcattaataa acaattgaga tttaggagtc aacatgagaa tcaataagaa ggaaaattat 120 a 121 <210> 166 <211> 121 <212> DNA <213> Citrullus lanatus <400> 166 agaaaagttt tgccacatgt caaattgtga ttgatcattt gtatggatga cactagaaaa 60 rataaatgca cccaagtatt tttttaaagt tatttgattt tctcttatac ccctaacatt 120 a 121 <210> 167 <211> 121 <212> DNA <213> Citrullus lanatus <400> 167 gtaaaatttt gaaacatgtg gaccaaataa aaattaaatt taaaagggta attgggttga 60 rtagcagttt tagagataat aattaagtgt ttaataacat ttttaaaaaa attacaaata 120 t 121 <210> 168 <211> 121 <212> DNA <213> Citrullus lanatus <400> 168 tttgttccca ataagttcaa acttatcaag aaggaagatc tttgttattt tcccgtaaag 60 ktggtcatca gaattttatg agaaaagtca caaagggctc aactccttcc tgataagttt 120 g 121 <210> 169 <211> 121 <212> DNA <213> Citrullus lanatus <400> 169 gcaattagtt tatctaataa aaaataataa taaattaatt gcaaattgga agctttgtgt 60 ytatggagga ttgggaaata ttgaaaaatt gttgttaaaa tttggagaag atgatgaatt 120 t 121 <210> 170 <211> 121 <212> DNA <213> Citrullus lanatus <400> 170 agattaatca atattcatta actataagtc actcgactaa agatttattg ttgcactcta 60 ygcactatag atatatttat gtgtccacgg aattaactga tcaacagtaa gccaaccctt 120 c 121 <210> 171 <211> 121 <212> DNA <213> Citrullus lanatus <400> 171 tgagaacgat ctcgtagtat ttcaattcct aatacaaaga agcattatca agatccttca 60 kctccaaatt cttttttaga aactttttag tgtcatgtaa taaacctaca tcattacttg 120 c 121 <210> 172 <211> 121 <212> DNA <213> Citrullus lanatus <400> 172 tcaatggtag aagatgaatt tttgaaccct acttcttcgt tcacttcatc ttttcatcaa 60 ycggccataa acaactacat tgatccattt tatcttcatc atatcgataa cactagcctt 120 g 121 <210> 173 <211> 121 <212> DNA <213> Citrullus lanatus <400> 173 actaaccatt tctacagtga cttttgctta gcttaacagt gatagtcatc taggctcccc 60 ytagatgtga gactccctct caagattact tagactccct ctaagtgtaa taatattagt 120 g 121 <210> 174 <211> 121 <212> DNA <213> Citrullus lanatus <400> 174 accggtacac ttcctacttg ccatatttct gctcatgtgt tattagatca tggagctacc 60 yattcttttg ttgctagcat gttttctgta aatattgatg gtaaattaga acctttgcct 120 g 121 <210> 175 <211> 121 <212> DNA <213> Citrullus lanatus <400> 175 gctgttgatt ctagattgat ctagagatca agtatgtaac aatcatatag tttgggtact 60 kataagttaa aatgcttgta atttacccct tttttttggt ttggtttata gttcgagtgc 120 t 121 <210> 176 <211> 121 <212> DNA <213> Citrullus lanatus <400> 176 ataaatattc caccaatttt aatgtaaata aaatgtttat ttataaagat ggtgttgata 60 ktcgttctat aaatttatag agtaatttat atgtgctaag accgttagca tctatagcac 120 t 121 <210> 177 <211> 121 <212> DNA <213> Citrullus lanatus <400> 177 tgttggtttg tataagtatg attagttcat tttgaagaat atacgtataa cgacaggatt 60 yaacgaaata acaataagaa aaaatgtatt tcattgacag tagaaaaaaa tgtcgttaaa 120 t 121 <210> 178 <211> 121 <212> DNA <213> Citrullus lanatus <400> 178 ggtctgtgaa aaactttgtg caactagtct tactttatat cttcttacct cattattttc 60 mttctttttc tcacaaatac tcatttgtat cccacaagtt tgacatcttc caaaaatatg 120 a 121 <210> 179 <211> 121 <212> DNA <213> Citrullus lanatus <400> 179 tttcgaacaa atatgttcaa gtaagtatga ccatctgctt gagagagtat gtgattaacg 60 ytaatcattt aacaaaaaaa gaattggttt tagagataaa gttaaacttt ttcatgaaga 120 a 121 <210> 180 <211> 121 <212> DNA <213> Citrullus lanatus <400> 180 tagctaagtg acttaacgtt cacaattgga tcctcagtgg aacaatattc ggacttcgtc 60 rtcttatatt ataacttgac atgtacactt acggttaaca cgaaaagtgt gcatcaccta 120 t 121 <210> 181 <211> 121 <212> DNA <213> Citrullus lanatus <400> 181 catgaaaaat tgagataatt gtaggaaaat cccaaaaaga tgggtcaaaa tgagatgtgg 60 ytcaattcta aaaactaatg ttatatgaca ttttgtttag agtgaaattg tcatgtttgt 120 c 121 <210> 182 <211> 121 <212> DNA <213> Citrullus lanatus <400> 182 tggtttggac actctaccct ttactttctt tgtctatgaa aatgttaagg taagtgaaat 60 ycttgttctt gagagcaaac ccatgtaatt tcttgagact ctatttttgt atgacatcca 120 t 121 <210> 183 <211> 121 <212> DNA <213> Citrullus lanatus <400> 183 gttgggaaag gcaaaggcaa ggacaaggcg gctaacaatg taataccctc tctccaaaaa 60 yctaggtatc caaaatggtt agtaaggata aaaggataaa agatgaggat taacggaact 120 t 121 <210> 184 <211> 121 <212> DNA <213> Citrullus lanatus <400> 184 aaaataattt tagtggtctt tctatcccta ccactatccg cagcccagtg acaaaatttc 60 rtagtcatat tccatgattg gatgcctcct aacatggtat aaactccgca accatggtag 120 a 121 <210> 185 <211> 121 <212> DNA <213> Citrullus lanatus <400> 185 atctatttac gataaactac tatagactat tatttgtttc aagacacaga tagcagtcta 60 ycacggtcta tcatttttct atatttgtta aaattaactt tagcaaaaat taaactaaaa 120 t 121 <210> 186 <211> 121 <212> DNA <213> Citrullus lanatus <400> 186 aaaaattata ctttaactaa tttctactct attattattg atttattttt ttgacgaaag 60 rttttatata attcattatt ttgacaaatt gtttgaatta agcaaggtgg acaacgggag 120 a 121 <210> 187 <211> 121 <212> DNA <213> Citrullus lanatus <400> 187 ttaacaaacg taataactac ttattcttaa tcaaataatt ttctcactca ttttgaacaa 60 watctcaact aattgcttgt tctttttttc ttttttgtaa tctttttttt tttttcaaat 120 a 121 <210> 188 <211> 121 <212> DNA <213> Citrullus lanatus <400> 188 aaaggtgtgt ttcaaagtga ttttattcat tttaaaatga ctctcaaaca tatactaaga 60 ytccaaatga gaaggactat agtagagata aaccgtgata ggttattttg ttagaaactt 120 a 121 <210> 189 <211> 121 <212> DNA <213> Citrullus lanatus <400> 189 tcgctcctct caatactcac tctcactttc aataattcaa catttgatct cgctattgtt 60 yacgttactt gttttcgctt cttgtttcgt atatttttaa tttgaacaga catataacgt 120 t 121 <210> 190 <211> 121 <212> DNA <213> Citrullus lanatus <400> 190 tttattgtta aaaatataga ccataacaaa tactttacgt caacaaatgt tataccacga 60 yacatttttt attgttacaa aattttgtat ttatgacact tttatattgt cagtaaaata 120 t 121 <210> 191 <211> 121 <212> DNA <213> Citrullus lanatus <400> 191 aaaaatgcta ttgcggtgcg ttaaaaagcg aataatgcca atttcaaatg gttgataaaa 60 ygagagaact aaagcttttt aaacacacac aatataaata tatataagtg atcgaccaat 120 a 121 <210> 192 <211> 121 <212> DNA <213> Citrullus lanatus <400> 192 ttaaaggcat tttaacactt ttatatcaaa tgtgctccac ttgtgtactt gtacttgcta 60 yaatttctta tcgtgccttg gtgaagctga gcatgagcat gagcatgaca tttatgaatg 120 t 121 <210> 193 <211> 121 <212> DNA <213> Citrullus lanatus <400> 193 ctgacggttc tattggcatc aaagaattcc ttcatttgta gaatctcaat ctaccaacac 60 maaaataccc atgcaagggt tttgtttgtt aagcttaagt tgttgttttg gctctctttt 120 t 121 <210> 194 <211> 121 <212> DNA <213> Citrullus lanatus <400> 194 cgtaaataac acgtaaacga atacaaatta ggttttgaga gcatttaccc ccaaggcttc 60 rccaagcgag acctcgtact tggtcatttg gagtgccaaa taacaaattc aagacatttc 120 a 121 <210> 195 <211> 121 <212> DNA <213> Citrullus lanatus <400> 195 ttggtaaatt taaaatttca gttacgttct caaggttaac ggtacatgcg tacaacacat 60 rttgtgtggt aggttgttaa ttaattatag gatgttgatg tgcttgagcg gaatgggaag 120 t 121 <210> 196 <211> 121 <212> DNA <213> Citrullus lanatus <400> 196 aatcagagaa tataattgaa gaacatgagt cgttaaacca aacaaataca agaatcaaaa 60 waaatcaata tgagggaaac ttcactcaca gggacattgg caagaaaaac atcccgaagt 120 a 121 <210> 197 <211> 121 <212> DNA <213> Citrullus lanatus <400> 197 ttttcctata ttaggaattc gtcaatgtaa caaaactaaa aaatccataa aagaaatagt 60 wttttctttt ctagaaaaaa taaatcaaga gacaattttg tttccatggt tcttcacaca 120 c 121 <210> 198 <211> 121 <212> DNA <213> Citrullus lanatus <400> 198 aatctaatat tttaatacgc ttaaccgtta cagatctaaa attaatttaa aatctaaaag 60 ktttccctat aaattaagta caatgatttt agttttaaga taaacaaaaa atcatcttta 120 c 121 <210> 199 <211> 121 <212> DNA <213> Citrullus lanatus <400> 199 agacaatatg aggtttgact tacacaaggt aaaatctcag ttgcagacca tcacgaccct 60 wcttagatca ttatgtcgag tatgaattta aataaattaa atgtcttaat ttttttttaa 120 t 121 <210> 200 <211> 121 <212> DNA <213> Citrullus lanatus <400> 200 acaagttcat tttctttcgc tctttttcat aatattttct tttcactgca aatgatttta 60 ycaactgatt tttaagtagg catataaata atttttcttc ctcagtatta cattacaacc 120 t 121 <210> 201 <211> 121 <212> DNA <213> Citrullus lanatus <400> 201 caaaatagtt accaaacaag gtcattgtta tagctatatg taaacaacca agagctcaat 60 ytaacataac ttagtgaata atgctattgg atctcctatt tgtcattatt gaactaaaaa 120 a 121 <210> 202 <211> 121 <212> DNA <213> Citrullus lanatus <400> 202 cttgctatgc catcatttgt gttgattttc tcttcatgag attcaaagaa gtttgctata 60 ytcaaatttg tcatattgga tgggtcaaat atatgtatgc aaaataaatt ttcttccaca 120 t 121 <210> 203 <211> 121 <212> DNA <213> Citrullus lanatus <400> 203 ttaatataat ctactaggaa ctttcgctag gtaaaaacat ggagaaatca aaaccttggt 60 yatggtacca tagctaaata gcttataaaa gaaacatggt ttacttaaca tcacttccac 120 a 121 <210> 204 <211> 121 <212> DNA <213> Citrullus lanatus <400> 204 aaggtggaag ttgtcgtact taactaaatt aatagtctaa tctcgtgggt gatactcgaa 60 ytcaattact atattaaaac taaataccgt atacttgtgg ttattcatgt tgtaaaaatc 120 a 121 <210> 205 <211> 121 <212> DNA <213> Citrullus lanatus <400> 205 acttatcttg atgagttatg ttatcttatg aggttataca acatatagtt aagtataaat 60 satctgtagt tgattaatgt tatagttaca gtaggaaaca ggtgtatgca ggttcgcact 120 t 121 <210> 206 <211> 121 <212> DNA <213> Citrullus lanatus <400> 206 tcataaaata agcatttaat aatgggacaa gattcatgaa ataaaataat atatcactta 60 rtaacgtatc aaatgcagaa tggaagtagt atatattatg gtagtactag aaattttcct 120 c 121 <210> 207 <211> 121 <212> DNA <213> Citrullus lanatus <400> 207 ctcttcaatt aaattaatca acattcatta actatgggtc actccacgac agacccacag 60 ytgcactctt tgtattatag acatatttat ctgtccacga atataaccaa tcaacagtga 120 g 121 <210> 208 <211> 121 <212> DNA <213> Citrullus lanatus <400> 208 ttgttttgtt gtttacgttt tacaagtgtt tttaaaattc aagtcaagtt ttaaaaacta 60 raagaaaaaa aatttcttaa aatttgacta agagtccaaa tgtttacctt agaaagatag 120 t 121 <210> 209 <211> 121 <212> DNA <213> Citrullus lanatus <400> 209 ctccatctcc aaaagtgacc tttctagtac tcattggttt gatatttgat aagaaggtcc 60 rttctccagt cacataacga gaatgaccac tgtcaaagta ccagtctcct tgagcagagg 120 a 121 <210> 210 <211> 121 <212> DNA <213> Citrullus lanatus <400> 210 attggatttg gtttaaaaca atgcaaacaa attacctaat gtaaccaagg tgaaattgtt 60 rttagttggt gacgcttaaa aaaataaaga caaacaatga tggaagtagg gtttcaacta 120 t 121 <210> 211 <211> 121 <212> DNA <213> Citrullus lanatus <400> 211 gcacattgtc tgtcaaattc aatatggaaa tagttgaatt taatattttt gatgctatga 60 ragctcccaa tcagagtcct cttattttat gtcaaattga tgtgattgat tgtttggaat 120 a 121 <210> 212 <211> 121 <212> DNA <213> Citrullus lanatus <400> 212 aacaatttgg attttagtat tcaatttgga catgtggccc ttgttttact tgagatgttt 60 wctgaaagtg aatatttgtt agtcaacagc ccttttagca tgtttaattt tcatttataa 120 g 121 <210> 213 <211> 121 <212> DNA <213> Citrullus lanatus <400> 213 atttagggag tgaatgctta attaaggact aatccactta ttttgcattc ataaactaat 60 wtaaactcaa gggaaaaatc gaatgttaga ttaggctatc cattctctca ttcgtcacaa 120 t 121 <210> 214 <211> 121 <212> DNA <213> Citrullus lanatus <400> 214 tttattactt tccttattta gttcatatta tccataattc ataatttcaa ttccaatcct 60 yacatacaat tgattcaact ctagaatagc taagtaactt taaccttcac aattggatcc 120 t 121 <210> 215 <211> 121 <212> DNA <213> Citrullus lanatus <400> 215 gacaatacgt aaataacatc aattcaaatc atatccaatc aaaatatgct cagtagcatt 60 ktcataccat ttatgttcat ttccgaaaaa gaacatgaaa attcagtata tttcaaaaac 120 a 121 <210> 216 <211> 121 <212> DNA <213> Citrullus lanatus <400> 216 aatgtattcc aaacacacta ttaattaaca aattcattta aatcatattt aaatgaacgt 60 mtccacataa cctatgattt taattcaatt aaatcaaatt gaattaaatt aaataatcaa 120 c 121 <210> 217 <211> 121 <212> DNA <213> Citrullus lanatus <400> 217 ctgttgaaaa tatttacaat taatagcaaa atacaccgtt tatttgtgat agatcgcgat 60 wgactactat ttgtgtctag tagtttattg cgatttatcg tagatagaca gtaaaatttt 120 a 121 <210> 218 <211> 121 <212> DNA <213> Citrullus lanatus <400> 218 tataatacta gttttacctt cactcattag cttaagcttt tgggtcaatt ggtgaattaa 60 rgtggcatta gagcaagtga tttagaggga ttctcgtgtt caaatcctgc tttattattt 120 a 121 <210> 219 <211> 121 <212> DNA <213> Citrullus lanatus <400> 219 gactgtagat cgtgagttat tgtatgttca cattcttgct aaattattca tctttacctt 60 yagagaaagg attcaacaat ttagggggag ccttataagt tcatcaagta gagtggtctt 120 c 121 <210> 220 <211> 121 <212> DNA <213> Citrullus lanatus <400> 220 tacagttagt agttagtaat agactgcagt agataccatt gatagacaac gaaattcttt 60 watatttgta aatagtttgg ttcattttgc tatatttgaa aacaaactaa tcttttatag 120 c 121 <210> 221 <211> 121 <212> DNA <213> Citrullus lanatus <400> 221 gatatttatg taagaatcaa cactgaaatt tatgtgattc attaacagtg tgttagatac 60 rtttacaggc aaagagtagt actgaaaaat ataagattat agcttcaaag aggcatctct 120 a 121 <210> 222 <211> 121 <212> DNA <213> Citrullus lanatus <400> 222 ttgcatgata atgaagaatt cttttagaag ttgctaaaag tggaggattg tgggaatgaa 60 kgttagaaat tgttgcataa taaaattgag ttattaattt aatcaagttg tttttcaata 120 g 121 <210> 223 <211> 121 <212> DNA <213> Citrullus lanatus <400> 223 ttgaacatct atcctcgaag ttaagagcat aagctttatg ccagttgaag ttaaactatg 60 stctttttgg catagagata agaaaatatt tagctctcgt tttggtggga gatgaataaa 120 a 121 <210> 224 <211> 121 <212> DNA <213> Citrullus lanatus <400> 224 ccaccctaga aagattttga tttttaggtt aaattataag tttggtccat taattttgag 60 rtatgcgact ttagtcctta aatttttaaa agtgtaattg atttgtacat tttcaatttt 120 t 121 <210> 225 <211> 121 <212> DNA <213> Citrullus lanatus <400> 225 aataaattta gctacaatca gaaggggaaa acactcctga ttgtgatgtt tacaggaact 60 magggggtat tgtgcactcc caatgcctac cacactgcat ttggttttcc ctctaacttg 120 c 121 <210> 226 <211> 121 <212> DNA <213> Citrullus lanatus <400> 226 atattgagtc ggcaaacggt cattctcacc catacaaatc aaaggacaat ccctcgtgga 60 yagaagttca taatatacta gagtttaaga ctaagttacc taggtcatcc aattggaata 120 g 121 <210> 227 <211> 121 <212> DNA <213> Citrullus lanatus <400> 227 atgatgatag tacaaatgtt aatttttaag tttcaattta aataactttt tctaataaat 60 waagaaaata caagattgaa caaatgacca cttgtttttg tttagtttgc tcccacaaag 120 c 121 <210> 228 <211> 121 <212> DNA <213> Citrullus lanatus <400> 228 acaattaaga cgatagagca attaaaatgc atgagttctc aaaataggtt ataaatctat 60 rttgaaatta atttcacatc aagtattaaa tatttgtgaa cccctaatag gcgctataca 120 c 121 <210> 229 <211> 121 <212> DNA <213> Citrullus lanatus <400> 229 gatggtatat caatgatatt acttaggtag atatcagtga tataacttag gtcaatatca 60 rcgatatcca tgtattagtg atatctgtga tagaacttag gtagatatca ttaatagaat 120 a 121 <210> 230 <211> 121 <212> DNA <213> Citrullus lanatus <400> 230 caattagatg gctatcaaaa ggctaactaa ttgacaaaaa aatagaaggt aattagatga 60 yaataaaaaa gcaatcacat aacaatcagt gagagcattt ttgatttttc agtttcaatg 120 t 121 <210> 231 <211> 121 <212> DNA <213> Citrullus lanatus <400> 231 aaagtaggtt aaagttaatt ttatcagcgg aaaaatcaaa tacttctcta atagttagtc 60 rtataattgt ttgtttgatc ttaatccttg tttaaaccac caataattca ctattagtac 120 a 121 <210> 232 <211> 121 <212> DNA <213> Citrullus lanatus <400> 232 atcatttgat ctgtatgggt ggagtggtta gatcgccgac tcaataagtc taccattttg 60 ragatttgtc tgagtaagaa actgggaaca tatcttcaca agatgaaatt cactacttcc 120 c 121 <210> 233 <211> 121 <212> DNA <213> Citrullus lanatus <400> 233 atgctttatt ctctttccgg aatttttctt ttactatctt aagatcatta cttaaccaac 60 rcattgttca ataaacttta atttaaaatc ttactttctg aatttacttt taaacttttc 120 t 121 <210> 234 <211> 121 <212> DNA <213> Citrullus lanatus <400> 234 aaatcctcat cggactcata ttacattact attcgttgag gtaactccat tgttctgtgt 60 ytgactacca caaagggttg aaagaacaat gatttttcgt tagtgacaat tggctcgccc 120 c 121 <210> 235 <211> 121 <212> DNA <213> Citrullus lanatus <400> 235 ctgtctttgc tcattgtaat ccgttgtgca aaatcgttgt tgaagagtaa aagatcaaga 60 rgataacttt tatcttaggg gatagcctaa ggcattgctt atgattagaa aatggaagat 120 a 121 <210> 236 <211> 121 <212> DNA <213> Citrullus lanatus <400> 236 caatttgaga aaatcgagag aagaagcgtg aaatggcggg ttgaacatga gctttttagt 60 yatttaccaa tatgactatt attgatagct gatatcagtg acatctagtg ctaattttgt 120 g 121 <210> 237 <211> 121 <212> DNA <213> Citrullus lanatus <400> 237 tattcatgtt catcattata aaatattttg aaacttaata gtggttaatt gaggtgtttg 60 yttaaatatt tttagtatat ctaaaaataa ctaatatatc gaatttgagc attttactta 120 c 121 <210> 238 <211> 121 <212> DNA <213> Citrullus lanatus <400> 238 gtggagacat ttaagtggag tatcttatag gaagaatttt tacaagacca aacaacaaaa 60 kaactaatct ctctttgtaa atctgtaaat tgatgagatt aatatttcaa aagatgatca 120 t 121 <210> 239 <211> 121 <212> DNA <213> Citrullus lanatus <400> 239 cagtttgatc acatgataaa tgttgaaaca tagatgtgaa aattagcata atttcagtac 60 racttacttc acaggtggac tatgcataaa tagaattcaa gggttgaatt tagatagaca 120 a 121 <210> 240 <211> 121 <212> DNA <213> Citrullus lanatus <400> 240 gagaagaaga atccaaacaa agaaaaaaat tgaagaaaaa attgtaatta tgtctaatga 60 rgaacaaaag gaagggacga gaacctagag aaagtagaac gaaaaactag aacgagagtc 120 a 121 <210> 241 <211> 121 <212> DNA <213> Citrullus lanatus <400> 241 aaggaccaag atcaacaaac taattattca gcacatcgac cagaatatac taaagtgtac 60 rtaggcgaat gctatctcat cgacacctat cgatttggaa gagacacgta tattgactaa 120 a 121 <210> 242 <211> 121 <212> DNA <213> Citrullus lanatus <400> 242 agttgatcaa gtcgtttgac ttcagcctca gccttgaaat atttttccac ataattagca 60 sttaattcat tagatcttct atttatcttt cacacgttga atagtagtac ctgctcaatt 120 a 121 <210> 243 <211> 121 <212> DNA <213> Citrullus lanatus <400> 243 attcttacaa ccttctaatc catgtcctcc gtcttcaaga aaggaactgc atgttaatta 60 sctaaattaa gtttttcaca ctttgtaacc ataataatta cttaatataa ttactaaaat 120 c 121 <210> 244 <211> 121 <212> DNA <213> Citrullus lanatus <400> 244 ttagaaaatt taactatata taaagaaaat atgaatgttt tcaaacctaa aaaaatgaga 60 saaaatattt agaaatacaa caaaattttg tgatagacat tgatagacga catctattag 120 t 121 <210> 245 <211> 121 <212> DNA <213> Citrullus lanatus <400> 245 cctttccatc aggtctgtat gagaatgtga aaacgggttg gatgttaatt tggataacgg 60 ratttccaac cctaccaatg ttttcatatg gtgaggttat accaattatg gaatagggca 120 g 121 <210> 246 <211> 121 <212> DNA <213> Citrullus lanatus <400> 246 tgtcatccaa agaagcatga tattgctccg aacaatgtgg tgttgagcct tgttcctctt 60 ktaagaaagc atcttcagcc aattggcatt cctcttttgt gcgtggaatc gtattggagt 120 a 121 <210> 247 <211> 121 <212> DNA <213> Citrullus lanatus <400> 247 ggggaaataa atgaaaaatt aatgtaagat tttcgattaa ttaatcaagt ctcataagtt 60 yataactcgt aattaatagg tgaatagttg acccttgaac tacacacatt aattaaataa 120 a 121 <210> 248 <211> 121 <212> DNA <213> Citrullus lanatus <400> 248 tagtgatatc ggctagtgag acccacaaaa atttcaacat aatcagattt agcattctcc 60 rtccttcaca tagagtgcat gtttttatta tagatgagga agtgagcctc atgtattagt 120 a 121 <210> 249 <211> 121 <212> DNA <213> Citrullus lanatus <400> 249 aataaaaaaa ataaaaaatt aacgctaatt taaaatatag tcgaaaacat ttgtattttt 60 kaaatattaa aatggggcaa attgaaaatg agaaaattca accaaagtga tgtaataata 120 t 121 <210> 250 <211> 121 <212> DNA <213> Citrullus lanatus <400> 250 tttgtgcctt ttgttttctg attgattgtg acaaaccaat gtttatgaat ttatattata 60 yttagttatt taaattacta acccattttt cattttgatt atattttaga ttgaaatatt 120 a 121 <210> 251 <211> 121 <212> DNA <213> Citrullus lanatus <400> 251 attggtggca ttcgtctaaa tagaatcact atgttgaaat tttaatttca gtttggttgc 60 yttcatatat tatcgataag tgatagctat gtggaaaaga ttatttaatt gaatgagcta 120 a 121 <210> 252 <211> 121 <212> DNA <213> Citrullus lanatus <400> 252 caaataaagg gagaaaaaaa aacactctac atacaatgag aatttgtgtt tgtaaattac 60 waaattgaat attataaatt aaatttagat gtacgaaaat tgcactctat attccattat 120 t 121 <210> 253 <211> 121 <212> DNA <213> Citrullus lanatus <400> 253 aactctaaat ttaactatgt tgtaaaacaa gaacaagaaa tgcaaattgg agagaaaaga 60 kagtatagaa atgaatgtat tctatattca actatgtctc ataaaggccc aatagagcct 120 a 121 <210> 254 <211> 121 <212> DNA <213> Citrullus lanatus <400> 254 actttctcac ttttgtccac tacacttggt ttgtttggag tttgaaaaac tccgtttgtt 60 rccctaagtc atcacatgta gtcagatcaa gaagaaacaa ctccacgttg ttatcggagg 120 c 121 <210> 255 <211> 121 <212> DNA <213> Citrullus lanatus <400> 255 ctaagatgaa gtgttgttga attatcaaac acgaaaatgt ccaaaaaaga ccattgtcat 60 matgacacgt gttagttaaa tttaataatg tgatttaaat tggttcggtt ggacgacatc 120 c 121 <210> 256 <211> 121 <212> DNA <213> Citrullus lanatus <400> 256 ccaaaattac acttgaaata ctattaatat acttgaacaa atgcattgtt aagaagtact 60 watttttttg acaatacacc aatattacat aacctaaaat gaattttgag ttaatgttgt 120 g 121 <210> 257 <211> 121 <212> DNA <213> Citrullus lanatus <400> 257 actatacatt atatatacat aattcacaag tttattgtta caaagtacaa agaaccaaca 60 saaaacttaa gaaattcaac ctccctctgt gtgcagaata tttaatgcca aggggtggat 120 t 121 <210> 258 <211> 121 <212> DNA <213> Citrullus lanatus <400> 258 ttatgactta tgagatatta aattgaaaaa tcaaattgaa gtaaaaatgt tggagttgga 60 rcttatttgt aagagtattc acatcagtat ggtgctgagt tgcgtatact ttgatgcctt 120 t 121 <210> 259 <211> 121 <212> DNA <213> Citrullus lanatus <400> 259 gaagaaagag ggagagaaga actagcgact gaattcgaag aatcttcaaa attcctgggt 60 ktcttcaatc tttggatttg atttctgtct tctttaacgg taagattcac cactgttttt 120 a 121 <210> 260 <211> 121 <212> DNA <213> Citrullus lanatus <400> 260 ttggccttgc cctagtggcg aaaaaaggtc aaggtcatta gttcaattcg tggtccctaa 60 yttaggattg aacattctat aagttatgat ccgtaagatt actcgaagtg cgtataagat 120 a 121 <210> 261 <211> 121 <212> DNA <213> Citrullus lanatus <400> 261 acatatcaag tcacatcaat cagacatttg aagcacaatc attttaaaat tggcacgaaa 60 ratacaggaa tattggagca aaaacaataa aatactcgtg tttgaaaatc tattgtcata 120 a 121 <210> 262 <211> 121 <212> DNA <213> Citrullus lanatus <400> 262 tttgtttata tgaacaaaat caaacattag ccatggtcat tcttttaaag aagatttttt 60 waaaaaaatt tttttaaaca aatttgtaaa taagttaaaa ggtcttgttt aaatagttat 120 t 121 <210> 263 <211> 121 <212> DNA <213> Citrullus lanatus <400> 263 tttttaatca ttcatgagtc atgcatcggt ttactctttt tttcatcttt tatttttttt 60 ygttttttga gtttaaaaat atgcaaagag gattcgaacc tttgatagct taatcgagga 120 t 121 <210> 264 <211> 121 <212> DNA <213> Citrullus lanatus <400> 264 actaaaaagg taagatgaac tccaatagca caatgagttt gaaactcaat aatttcatac 60 ygaatgaaag ttcctgtgct atatgaaatt cccgacaaag cttgaaaatg aaaattacgg 120 t 121 <210> 265 <211> 121 <212> DNA <213> Citrullus lanatus <400> 265 atgatttatc aatcaaacct aaattactca ttttgcatgc ttaataaatt tatgaaattt 60 ytatttaaca caaggttgaa agttcaagga tttattaaat acgaaattaa aagtataaaa 120 a 121 <210> 266 <211> 121 <212> DNA <213> Citrullus lanatus <400> 266 acgatcacat taattgagaa ttagagattt ctattaagga gattctgtta aatttaaatg 60 raatttaatc agtcttacta gtatattcca tatattggtt agaatatttg atagtactca 120 a 121 <210> 267 <211> 121 <212> DNA <213> Citrullus lanatus <400> 267 gtaaaacgac ctgggtcgag taacatcttc aggggattgg tgtcaattat tttttctatt 60 ygcaaaattg ttttaatttt atttaggaga ctgactttaa tgtaaattaa tctaaaccta 120 a 121 <210> 268 <211> 121 <212> DNA <213> Citrullus lanatus <400> 268 agctacactt ggttgatctg ttgtcgacta gaattcttaa gcacaaaatt ctaaaatttt 60 kgtcacctta gttttttgca ggacaacaat ttctgtagta acgtgttgat cttctccact 120 c 121 <210> 269 <211> 121 <212> DNA <213> Citrullus lanatus <400> 269 catgccaaat tgtaaatata tctggattca tgaacttcgg gttcattgtt gcatatgttt 60 yaatgactcg tatatgagta ataatataat ctagaaaata aagtaggcaa acttgttcaa 120 t 121 <210> 270 <211> 121 <212> DNA <213> Citrullus lanatus <400> 270 ccagtggtaa ccagaaatat ttgagaaagg tgtttgtttc taatctaaag aaagcgataa 60 matgtatgct agactaaaat gtggaagatg gaaaggcatg tgataacttg tagatttaca 120 a 121 <210> 271 <211> 121 <212> DNA <213> Citrullus lanatus <400> 271 acttaaatta aaccttttca tgtattatac taactaagag atcgttcaat ttacactaag 60 ygatcgctta atatacaata aacgatgact taatagatat tactaagtaa tcacttaaac 120 a 121 <210> 272 <211> 121 <212> DNA <213> Citrullus lanatus <400> 272 taaaagttca aagtttaaat tgatacaatt attaattcat gtcttaagtt gatacaacct 60 ygggttacca attgatattt tttcctaaat tatgcaagat atactatttt ttcgttcttt 120 t 121 <210> 273 <211> 121 <212> DNA <213> Citrullus lanatus <400> 273 tccctgacag tatggtgact aacaattccc tcaacgttac aaaactcatt gaacttagac 60 scacaaaatt tcagactatt atcagcttac aacattttga tcttcttccc agtttgattt 120 t 121 <210> 274 <211> 121 <212> DNA <213> Citrullus lanatus <400> 274 acatccgtgc ctatttattt caactgacat taatggtatg atttcttcta gtgaacatgt 60 ygaacatatg tgcattagtg gataccatta ttgatattat cattatgtta tttgatactc 120 t 121 <210> 275 <211> 121 <212> DNA <213> Citrullus lanatus <400> 275 attgtacagt gttcaatgta tacattcgac ctgtaagtgg cccatttgag caattaaaat 60 kgagggatgt gatattttta atgtaacacc cccatcctag atatttatat atacatctat 120 a 121 <210> 276 <211> 121 <212> DNA <213> Citrullus lanatus <400> 276 attgtttagg tgcgttagac gactcataca ttaaagtgaa tgttagtgcc gtcgatcaac 60 stcgatatag gatgaggaag ggagaaattg ccacaaacat tcttgcagtg tgtgatctgg 120 g 121 <210> 277 <211> 121 <212> DNA <213> Citrullus lanatus <400> 277 ttttgtaatt attagtttta ggagaaatga tacattttgt aattgtttga ttttgataac 60 rtacccatat gtgattgaaa tactgaagtt gtgtagttag tgatttatag gagatgtgta 120 g 121 <210> 278 <211> 121 <212> DNA <213> Citrullus lanatus <400> 278 tcacaataac ataacataaa attgagaaaa gttcataata ttctcaaggt ctacttctac 60 rtctccccaa cttttgcacg aatgatctaa aaaattttaa tgaactaact ttttcaaaag 120 c 121 <210> 279 <211> 121 <212> DNA <213> Citrullus lanatus <400> 279 gttttagggt gttggatgat atattatgta tttaagttaa tgattttagt gtttttcatg 60 ratgttgttg aatgatatgt ttttcaatat tattgtgctt tattctttat tgattgatca 120 t 121 <210> 280 <211> 121 <212> DNA <213> Citrullus lanatus <400> 280 ggattaaggt cgaccctgat aaagtcaaag ctattttaga aaggccacca ccacgcaccc 60 raaaagaagt taaaggactt ttagggagac taaactatat cgcgagattt atatcacatc 120 t 121 <210> 281 <211> 121 <212> DNA <213> Citrullus lanatus <400> 281 ttacaaataa tagcaaaata tcacaatcta tgtataatag atcgagatag actactatct 60 rtgtctatta tttcacagat agaaacagat agttgtctat tgcaactaac agtaaaattt 120 t 121 <210> 282 <211> 121 <212> DNA <213> Citrullus lanatus <400> 282 ctacaaactc aagtttgtga tagaggtgtt catgggttgg attgggctga atgacttttt 60 rgacccaacc caacccaatt gttcagattc taaatttctt caacccaaat aactcttatt 120 a 121 <210> 283 <211> 121 <212> DNA <213> Citrullus lanatus <400> 283 tagttttgag cgagctagta attacttgtg ggtggtactt gtcttgcatg gctgtacaga 60 kaattgtgtc gagctagtca tgcacaagct ttggatatag cagacccact gatactactt 120 c 121 <210> 284 <211> 121 <212> DNA <213> Citrullus lanatus <400> 284 tgttagtcat agattctgta agaaaaatca tctccctgat ctaaggaaat aaaaggtagt 60 kaggtgtgtg aaagatgggt attctttttc ttctttaact aatagattaa acttttttca 120 a 121 <210> 285 <211> 121 <212> DNA <213> Citrullus lanatus <400> 285 agcatgcgtt ctgtctttag aggtcggatg ttcaattctc tatctcacag atgttgtata 60 raaaaagaaa aaaaaaggac aaaaagattc cttagcatta gcatttactt agcctgaatc 120 c 121 <210> 286 <211> 121 <212> DNA <213> Citrullus lanatus <400> 286 aatgacatct aataatttaa aatagcatct tattattaat gatgatctaa gaaacaaaaa 60 ytttaaacca atatattgag aaacagggtt ttagtttata tttttagaaa tttcaaatat 120 g 121 <210> 287 <211> 121 <212> DNA <213> Citrullus lanatus <400> 287 aatgacatct aataatttaa aatagcatct tattattaat gatgatctaa gaaacaaaaa 60 ytttaaacca atatattgag aaacagggtt ttagtttata tttttagaaa tttcaaatat 120 g 121 <210> 288 <211> 121 <212> DNA <213> Citrullus lanatus <400> 288 ctatctgata gaaatgaaaa gaaactaagg attctacaaa tataaccatt ttcgtctttt 60 ygaaaaaagg gggcaggcaa gaagagaaca ctttaaaatc ttaatttgtg aatgaaaagt 120 g 121 <210> 289 <211> 121 <212> DNA <213> Citrullus lanatus <400> 289 tggtagacca atgaaaagtc ttagaaaaag aaaaatagac aattgtaata aaagacctga 60 ytaaggatgt aaacatccat gaagaaaccc tggacatgac tgatagtcga attagtaaaa 120 a 121 <210> 290 <211> 121 <212> DNA <213> Citrullus lanatus <400> 290 tcgctgttca attgttcttg atattccttc caagactgta tgtttacttc aatgaatgca 60 wttaagcctt tctttttgta ttgttcctct gatttctgtc ttctcttttc tctttccttc 120 c 121 <210> 291 <211> 121 <212> DNA <213> Citrullus lanatus <400> 291 cacttacaag catgctctta gggcatattt gatagtgatt ctaaaatgag caaaatcact 60 yttgttatat tcactctaaa acatgaaaca tgtttttaat cattcaaaac ctatttgatg 120 a 121 <210> 292 <211> 121 <212> DNA <213> Citrullus lanatus <400> 292 gttttctttg atttgataaa aaatttcttc cggttaacta atgggggttt tgtgattgtt 60 ktgattgcca gagattttag atgcaaggat gtgaacttgc ataaaagtaa agcaagaatt 120 g 121 <210> 293 <211> 121 <212> DNA <213> Citrullus lanatus <400> 293 gtacagaggt gcaagcagtt cggagaagaa cttagaaagg agatttggac acaagtaaca 60 rctagcttgg agtggatgga attttttaga ctcgttaaag caataatgaa ggtagagaag 120 a 121 <210> 294 <211> 121 <212> DNA <213> Citrullus lanatus <400> 294 tttctggtta ttgctcattt gttgaaggaa acttagttac ttggcatagt aaaaaacaaa 60 rtgtagttgc aagtagtgct gaagcaaaat ttagggcatt agcccatggt atttgtgaag 120 g 121 <210> 295 <211> 121 <212> DNA <213> Citrullus lanatus <400> 295 atttaggcac atcaaaactc aaatgtgaat agcggatatt aattaattat ttccaacctt 60 rccctatatc cgtacaaagt ggcaagaaag tcacttgctt tagtgaaact cacaataaac 120 a 121 <210> 296 <211> 121 <212> DNA <213> Citrullus lanatus <400> 296 ggatatatga ttattgcttt atgattaatg ttcaattctt tgcatttgat tgcatgttaa 60 ytgtaggaag cagtaggtta ggttagcttg cgaatcaaat tagataagta ttgatctaac 120 c 121 <210> 297 <211> 121 <212> DNA <213> Citrullus lanatus <400> 297 accttttggg actttcaacc catttctcct ccatatagca atctcttaat actctagaat 60 ytgaagccga accttcccat ctaggtaaga tataaataaa ttcaatattt tgactacaaa 120 c 121 <210> 298 <211> 121 <212> DNA <213> Citrullus lanatus <400> 298 agtttccttc aattccacag taatgcggtt gaataaaata gagtacacta taactaccct 60 yttgaatgag ttatagactt attagtctct tattaaaact aagggaccaa tcgaaagagt 120 t 121 <210> 299 <211> 121 <212> DNA <213> Citrullus lanatus <400> 299 taaagtccat ttctatagtt tttttacatt attatctaat ttgtgtgatt tagggtaaaa 60 mttaaaccca acctatgtga tgtgatttct aggaaaaaaa aaaatgaccc ataaggatga 120 t 121 <210> 300 <211> 121 <212> DNA <213> Citrullus lanatus <400> 300 tttgttatga catttccatc tccaattaga taaataggac catcatcaat tcatgtcggt 60 rttgtgtcat aaaatgaatc gtaattcttt tctgtctaac tatgacactt aaaagactta 120 t 121 <210> 301 <211> 121 <212> DNA <213> Citrullus lanatus <400> 301 acattttagc cgttagagcg gcaagggaga acttcaatgt cccctcggtt ctgatatgaa 60 rcattgatta gggcatcaga gaaaagagga ggtgtgagag aagaatacac aacatctgga 120 a 121 <210> 302 <211> 121 <212> DNA <213> Citrullus lanatus <400> 302 aactgaacat aacgtaccaa gaaagagaca tacgcatata aagagttctc tattgtaatc 60 rattggctct attgactaca cccaaactta gctcctttct ttggataatg gtctttccaa 120 a 121 <210> 303 <211> 121 <212> DNA <213> Citrullus lanatus <400> 303 ttagaaaaca gtatgtagtc gaattatggg cagacaaatt tttatggtaa tcaatgaagt 60 kaatattcgt ttatacagtt gtttcaatta aatttttcat gtattatact aactatgcga 120 t 121 <210> 304 <211> 121 <212> DNA <213> Citrullus lanatus <400> 304 actcaatcac tcaaaagcat aatgttcaat taaagaaagt aagaaaatag aaacaatgaa 60 yaaaatcatc atagaaattt acatgattta ctaacagtat gctatgctat gtctaatgag 120 t 121 <210> 305 <211> 121 <212> DNA <213> Citrullus lanatus <400> 305 taactcaatt taggtgacat atagaatgtg tcaagatcat agagtttgtg attcaaattt 60 yccacattta ttatactaaa gaaataaaat aaaacaaacc ccagcttatt tatataaata 120 a 121 <210> 306 <211> 121 <212> DNA <213> Citrullus lanatus <400> 306 gtacaatgta aacggtctag atgatttttt tatctagaac aaattgcaaa aaccatatgt 60 raactataga tagttataat tacaccctca aacttttaat tgttaaaatt aaactcctaa 120 a 121 <210> 307 <211> 121 <212> DNA <213> Citrullus lanatus <400> 307 gtggagttgt gatttaactc cactccttgt ttcgctcaag gagttcatcg gttccacaac 60 waaaatacat caattttatg tattgattta actccttatg ttttgtggct caggagttca 120 c 121 <210> 308 <211> 121 <212> DNA <213> Citrullus lanatus <400> 308 taaaaatgtc tctcactttg tattcaagtt atataacttt ttttcatgtt atcaatttca 60 wtagtaatgt taaaatgtaa aacttataga ccaagtcata aaatttaaag attaaatagt 120 a 121 <210> 309 <211> 121 <212> DNA <213> Citrullus lanatus <400> 309 tgcccagcac actaactttt tttaattatg attattatta tttatagaat ttttatacat 60 sgatgatgat atttgaaagt tttatttgtt gcacgaccta ctcgaattat tacctcttct 120 a 121 <210> 310 <211> 121 <212> DNA <213> Citrullus lanatus <400> 310 tttgaaaaaa taatcttttc cctacatttt tatatgttat tttctaccct tcatatacct 60 rttttgaggc ccaattttaa ccattacaat ctataaattt gaaggtttat ttaatttcat 120 t 121 <210> 311 <211> 121 <212> DNA <213> Citrullus lanatus <400> 311 tagaactaaa ctaaaaatga attaggttaa agaatattaa aatacctcga attaaattga 60 raaatttagg gcgagtcatt aatgatcttc ttcttcgaac tctaaggtta aatctcaaag 120 g 121 <210> 312 <211> 121 <212> DNA <213> Citrullus lanatus <400> 312 taaatattta gaaaaattaa ttaaaaaatc tacatgtatg cattcttttc atttcattgt 60 ygaggcaaag tcgcattcat caactaccct ctattttctt tgtatcaaat aacattttgc 120 t 121 <210> 313 <211> 121 <212> DNA <213> Citrullus lanatus <400> 313 ttggatttta atagtttttt caatttttta ataaaaaatt acttccatca accctaatat 60 wgaggtttta ctaatgttgt aattttctta gaactaattt aagattttac taaaattttg 120 a 121 <210> 314 <211> 121 <212> DNA <213> Citrullus lanatus <400> 314 gttttctctg atgtcatgta gatgacggag tcaggtatca gaatgaagaa caatttcatc 60 watttgattg atttccgaat tttcaagatc cttagaattt gatgatagta tgtgcgtgat 120 t 121 <210> 315 <211> 121 <212> DNA <213> Citrullus lanatus <400> 315 atttattcca cctctataag ggtttgttat ctaattttta ccaatttttt caacaaccaa 60 rctctatttt ataaactaaa aaaagtagct tttaaaactt atttttattt ttgaaatttg 120 g 121 <210> 316 <211> 121 <212> DNA <213> Citrullus lanatus <400> 316 ttacattaca agacgagtcc attagaagcc cctaaaagga ccacatggta tacaaatatc 60 satatgtgat gtacactaca agtacaattg aaaaaatgac agaacaaact gtgcagtttc 120 t 121 <210> 317 <211> 121 <212> DNA <213> Citrullus lanatus <400> 317 aaaattgagg tagaaagaaa aacaaaccaa aaggtaggac ataaattttc tttagaaaaa 60 raaatatatg acctgtttga taacattcat gtttcctgtt tcctgttttt ctttccttgt 120 t 121 <210> 318 <211> 121 <212> DNA <213> Citrullus lanatus <400> 318 tttaatgaaa tttatgccct ttcgagagca attagagttt aaatttcata atattcacat 60 ytcaaaatag ggtccaattg cttagctcgt tggacaacag acgagtctaa atctcattgt 120 c 121 <210> 319 <211> 121 <212> DNA <213> Citrullus lanatus <400> 319 tgcacagttt tattctatga cttatacttt agtctaaaag gttggtaagt gatgaataat 60 raaaactaaa cgtgataaga tatttgtaat atagtttaac atgtgagcca tatgatgcga 120 t 121 <210> 320 <211> 121 <212> DNA <213> Citrullus lanatus <400> 320 cgaggatgag ttttcttcac ttgcacgaag ggaatttaat gtagtttatt atgttcaaca 60 rctgtttttt gaaattaata gcagtgtaat tctacctttt catacttctt tgtaattttc 120 a 121 <210> 321 <211> 121 <212> DNA <213> Citrullus lanatus <400> 321 ttattatcta tgagatttga atcatcatgt ttgccagatt atacaaaaca ccctcttttt 60 wcttgtctgc tttcattctt taaacgaaca agaataaaac tttccattga aatgaagaaa 120 a 121 <210> 322 <211> 121 <212> DNA <213> Citrullus lanatus <400> 322 caattagatt gagatttgtt agttttaagt tcagagtcta ctttttataa tccaaatttt 60 ktctaattgt tgggaaacaa tttttgatgt gttcgagagt tgtttgttca tacgcatttt 120 t 121 <210> 323 <211> 121 <212> DNA <213> Citrullus lanatus <400> 323 agaataattt atatactcca tttaataagt ctacgttgga gacagagagt tcacttcaca 60 rtacagaatt tactttttct ctataaagaa agtagataag tagtttcctt aaatttttta 120 t 121 <210> 324 <211> 121 <212> DNA <213> Citrullus lanatus <400> 324 tagtgcgaca ttacaaagga aattgcctcc aaaactaagg atccacagag tttctctatc 60 mcttgtcatt taggcaataa taagattact aaagcactat gtgatcttgg agcttccatt 120 a 121 <210> 325 <211> 121 <212> DNA <213> Citrullus lanatus <400> 325 tgtagatttt gaatatttat ctaatccaca caaagctaga tctcaaacag gttatctgtt 60 yacattggga agaattgcta tatcatggcg atcagtgaaa cagactataa cgactacttc 120 c 121 <210> 326 <211> 121 <212> DNA <213> Citrullus lanatus <400> 326 tctcacccac ttttactatt aaatttcttt aattcatttt ctactctcaa atttatctac 60 ycaatctact ctcttacgtt atatcatgtt atttgttaat gatcaattta gacctacaat 120 g 121 <210> 327 <211> 121 <212> DNA <213> Citrullus lanatus <400> 327 atcacgtgca gcactacaac tttgtcaaaa tttgcttcct attgatttta gccccgcgca 60 ygacgctcta ccttagggga aattttgtaa ttttttactc tttttggcct aattgcttca 120 t 121 <210> 328 <211> 121 <212> DNA <213> Citrullus lanatus <400> 328 ttagttgcca tgaagtagga aattaccaaa gcttttatga tataacatgc ttgtatgaga 60 ytttcatgct taaaagtaga aattcccaag acctttacga tatagcatgc ttgcatgaga 120 t 121 <210> 329 <211> 121 <212> DNA <213> Citrullus lanatus <400> 329 ctcaagtaat aacctaaaag gtttgcagta tatggataag gttgggtgtt ttatccttgt 60 racactacag atacaactca ctttgtaaat gttacaagag ttgtaagtgt tacaaatgat 120 c 121 <210> 330 <211> 121 <212> DNA <213> Citrullus lanatus <400> 330 actgattata tatacttgaa ttgcattgac atgctaggcg aatttagcaa aaaaataatt 60 raataaataa aaatgaaatt gaaaatttgc atgtaattta tgtttatggt acaaattatt 120 t 121 <210> 331 <211> 121 <212> DNA <213> Citrullus lanatus <400> 331 tggctaagta cacaaatttc acgcacacct acatgtaatt ggagatgaga aggcaaccaa 60 katgactttt ataaaatgaa agtaaaagat caatttatac ttctaaactt taggtattgt 120 g 121 <210> 332 <211> 121 <212> DNA <213> Citrullus lanatus <400> 332 ctttcccact atttatccag atgagtcaag ggttagaaat attggtagac catgtacttc 60 ycgaataccg aacgagatgg attggaggga ggtagaagta ttggtagacc atgtacttcc 120 c 121 <210> 333 <211> 121 <212> DNA <213> Citrullus lanatus <400> 333 tcttctctca attcttttat attcttctac tcaaaagtaa tttttgtgag tttaagcctc 60 kctttataag ctgatgtttg cttaagtttt taccacaaat ttgtaactga ttttgtttca 120 t 121 <210> 334 <211> 121 <212> DNA <213> Citrullus lanatus <400> 334 ctttagatgt ttagtcgtta taaacacttt gttgattttt tagaaccact aaaaataatc 60 kgttattcta taatattttt tcatgataag acttacaggt aattcttggt tatactttat 120 t 121 <210> 335 <211> 121 <212> DNA <213> Citrullus lanatus <400> 335 tatacaaaaa tttcagtcat aaaaagaact atagaagtct atcgtgttct gttgctaaaa 60 wtttttcata tttgtaaata gtttaatatt ttttccattt tatcataatt tccttttgat 120 t 121 <210> 336 <211> 121 <212> DNA <213> Citrullus lanatus <400> 336 acccaggtag aattccaaaa tcaaatttca tcttaaaaaa gtttgagctt ggattcaaaa 60 ycttgaatag ttaaaatact attttacttt ttttactttg gagtttgttt aattttagtt 120 t 121 <210> 337 <211> 121 <212> DNA <213> Citrullus lanatus <400> 337 agacttaatt aactacttat atttgtccat tgactttatt tggtgataga agtttatcgc 60 ygataaaatt caatcaggat atgtgtagtt tattagtgat agaagcctat cattgataga 120 c 121 <210> 338 <211> 121 <212> DNA <213> Citrullus lanatus <400> 338 taaatgacca atgcagggtt caaacaatag tattcaagtc aaacatggaa aattttggta 60 rttaaaaata gaacgcatga cgttcaatgg tcgagacctc ctactattac accaaacaca 120 t 121 <210> 339 <211> 121 <212> DNA <213> Citrullus lanatus <400> 339 acgttatttg ttatttgttt ttagtatata taaattagaa agtaaaaaat cgtagaaggc 60 rgtgggaccc gctttctcag gctaaacgcg tcgacagggt tcggtggttg ggtcacgcgg 120 t 121 <210> 340 <211> 121 <212> DNA <213> Citrullus lanatus <400> 340 attaataaat taaggtcggt tcttattaat attcatctat ttgagtccaa cctaacccaa 60 yccaaatcat aagaattgag ttgggttaat cagatttttt tatggggtat tttaacaccc 120 c 121 <210> 341 <211> 121 <212> DNA <213> Citrullus lanatus <400> 341 atgtatgttg tgctacaaag gatactgagt ggttaagaaa gaataggaag taaactttca 60 wggaaaatca tattctgaag taaaatattg cttctgacag aaggattaga gtaaaaaaac 120 t 121

Claims (6)

  1. 서열번호 1 내지 341의 염기서열에서 각 염기서열의 61번째 염기에 위치한 단일염기다형성 (single nucleotide polymorphism, SNP) 전부를 검출하기 위한 제제를 포함하는, 수박의 여교배 세대단축 품종 식별용 조성물.
  2. 제 1 항에 있어서,
    상기 제제는 서열번호 1 내지 341번의 염기서열에서 각 염기서열의 61번째 염기에 위치한 단일염기다형성 부위를 포함하는 5 내지 121개의 연속염기, 또는 이와 상보적인 염기서열로 이루어진 폴리뉴클레오티드를 포함하는, 수박의 여교배 세대단축 품종 식별용 조성물.
  3. 제 1 항에 있어서,
    상기 제제는 프로브, 프라이머, 및 이들의 조합 중 어느 하나인 것인, 수박의 여교배 세대단축 품종 식별용 조성물.
  4. 제 1 항의 조성물을 포함하는, 수박의 여교배 세대단축 품종 식별용 키트.
  5. 제 4 항에 있어서, 상기 키트는 PCR 키트, 마이크로어레이, 또는 플루이다임 SNP 분석용 키트인 것인, 수박의 여교배 세대단축 품종 식별용 키트.
  6. a) 반복친 수박 시료로부터 폴리뉴클레오티드를 분리하고, 서열번호 1 내지 341번으로 표시되는 각각의 염기서열의 61번째 위치에 존재하는 단일염기다형성 전부의 유전자형을 결정하는 단계;
    b) 여교배 후대 수박 시료로부터 폴리뉴클레오티드를 분리하고, 상기 a)단계의 단일염기다형성의 유전자형을 결정하는 단계;
    c) 상기 a)단계에서 결정된 반복친의 단일염기다형성 유전자형과 상기 b)단계에서 결정된 여교배 후대의 단일염기다형성 유전자형을 비교하는 단계; 및
    d) 여교배 후대 중에서 반복친과 단일염기다형성 유전자형이 일치하는 비율이 높은 여교배 후대를 선별하는 단계를 포함하는, 수박의 여교배 세대단축 후대 선별 방법.
KR1020210079294A 2021-06-18 2021-06-18 수박의 여교배 세대단축 육종을 위한 단일염기 다형성 마커세트 및 이의 용도 KR102578134B1 (ko)

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KR20180077873A (ko) * 2016-12-29 2018-07-09 부산대학교 산학협력단 수박 분자마커이용여교잡 선발용 snp 마커
KR102125528B1 (ko) 2019-06-18 2020-07-07 대한민국 오이의 여교배 세대단축 육종을 위한 단일염기 다형성 마커세트 및 이의 용도
KR102235665B1 (ko) * 2019-11-18 2021-04-02 한국생명공학연구원 참외의 마커이용여교배 육종을 위한 단일염기다형성 마커 세트 및 이의 용도
KR102273611B1 (ko) * 2020-06-04 2021-07-06 농업회사법인 홍익바이오 주식회사 흰가루병 저항성 호박 자원을 판별하기 위한 분자마커 및 이의 용도

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20180077873A (ko) * 2016-12-29 2018-07-09 부산대학교 산학협력단 수박 분자마커이용여교잡 선발용 snp 마커
KR102125528B1 (ko) 2019-06-18 2020-07-07 대한민국 오이의 여교배 세대단축 육종을 위한 단일염기 다형성 마커세트 및 이의 용도
KR102235665B1 (ko) * 2019-11-18 2021-04-02 한국생명공학연구원 참외의 마커이용여교배 육종을 위한 단일염기다형성 마커 세트 및 이의 용도
KR102273611B1 (ko) * 2020-06-04 2021-07-06 농업회사법인 홍익바이오 주식회사 흰가루병 저항성 호박 자원을 판별하기 위한 분자마커 및 이의 용도

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