KR20220058461A - 모노머형 스트렙타비딘을 발현하는 균주 및 비오틴화된 화합물을 포함하는 암 세포 표적용 조성물 - Google Patents
모노머형 스트렙타비딘을 발현하는 균주 및 비오틴화된 화합물을 포함하는 암 세포 표적용 조성물 Download PDFInfo
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Abstract
본 발명은 모노머형 스트렙타비딘을 발현하는 숙주 세포에 관한 것으로서, 본 발명에 따른 숙주 세포는 체내에서 스트렙타비딘을 발현하여, 비오틴화한 진단제로 숙주 세포가 예비 표적한 암조직을 실시간으로 모니터링할 수 있을 뿐만 아니라 비오틴화한 항암제의 암 표적 효율을 높일 수 있다.
Description
본 발명은 모노머형 스트렙타비딘을 발현하는 균주 및 비오틴화된 화합물을 포함하여 암 세포를 예비 표적화 할 수 있고, 이에 따라 암의 진단이나 암의 예방 또는 치료가 가능한 다양한 용도의 조성물에 관한 것이다.
암은 현재 전세계적으로 가장 많은 사망자를 내는 질병 중 하나로서, 평균 수명의 연장과 암 발생 연령이 낮아짐에 의해 암 발생률은 계속적으로 증가되고 있는 추세이다. 한국의 국립 암센터(National Cancer Center)에서 제공하는 2013년 통계 자료에 따르면, 2010년 암 등록 통계과에 등록된 우리 나라 암 발생 환자는 모두 202,053 명이며 계속적으로 증가되는 추세이다.
따라서, 암에 관해서는 세포수준의 기본연구에서부터 전방위적인 항암치료에 대한 연구가 전 세계적으로 추진되어 왔으나, 아직까지 암의 발생기전이 불명확하고, 재발방지 및 완치가 어려워 항암제에 대한 수요가 폭발적으로 늘어나고 있으며, 막대한 연구비가 연구소 및 기업체에 투자되고 있다. 하지만, 고가의 암 진단 및 항암치료 비용은 직접적인 의료비용일 뿐 아니라 발병 이후의 사회 경제활동의 위축, 재활 및 환자 간호에 따른 간접비용이 추가되어 암환자 가족 및 사회 구성원 전체에게 경제적으로 큰 부담이 되고 있으며 저비용의 새로운 기술도입이 요구되고 있는 실정이다.
한편, 스트렙타비딘(streptavidin) 및 아비딘(avidin) 단백질은 비오틴(biotin)에 대하여 높은 결합력을 갖는 단백질이며 비오틴과의 특이적인 상호작용을 이용한다면, 비오틴을 발현하는 종양에 특이적으로 반응하는 항암제 또는 면역 세포를 활용할 수 있는 등, 다양한 생물학적 응용분야에 적용될 수 있다.
그러나 이 단백질의 테트라머 형태는 비오틴 컨쥬게이트의 원치 않는 가교 결합을 초래할 수 있어 비오틴 활성이 유지되는 모노머형 스트렙타비딘 개발이 요구되고 있다. 최근 아비딘 중 하나인 리자비딘에 돌연변이를 도입시켜 모노머화 시키거나 스트렙타비딘과 리자비딘 서열을 융합하여 모노머형 단백질을 개발하는 등 모노머형 아비딘 유사 단백질이 개발되어 보고되고 있다. 그러나 생물학적 응용 분야에 활용하기 위해서는 정제과정을 거쳐 가용성을 담보하는 순도 높은 단백질을 얻어야 한다. 또한 아비딘 유사 단백질은 생체 내 주입시 혈청에서 신속하게 분해되어 임상 연구의 이용이 제한적이게 되는 문제가 있다.
본 발명의 일 목적은 모노머형 스트렙타비딘(monomeric streptavidin; mSA)을 발현하는 균주 및 선택적으로 비오틴화된 화합물을 포함하는 암의 진단을 위한 조성물을 제공하는 것이다.
본 발명의 다른 목적은 모노머형 스트렙타비딘(mSA)을 발현하는 균주 및 선택적으로 비오틴화된 화합물을 포함하는 암의 예방 또는 치료를 위한 약학적 조성물을 제공하는 것이다.
그러나 본 발명이 이루고자 하는 기술적 과제는 이상에서 언급한 과제에 제한되지 않으며, 언급되지 않은 또 다른 과제들은 아래의 기재로부터 당 업계에서 통상의 지식을 가진 자에게 명확하게 이해될 수 있을 것이다.
이하, 본원에 기재된 다양한 구현예가 도면을 참조로 기재된다. 하기 설명에서, 본 발명의 완전한 이해를 위해서, 다양한 특이적 상세사항, 예컨대, 특이적 형태, 조성물 및 공정 등이 기재되어 있다. 그러나, 특정의 구현예는 이들 특이적 상세 사항 중 하나 이상 없이, 또는 다른 공지된 방법 및 형태와 함께 실행될 수 있다. 다른 예에서, 공지된 공정 및 제조 기술은 본 발명을 불필요하게 모호하게 하지 않게 하기 위해서, 특정의 상세사항으로 기재되지 않는다. "한 가지 구현예" 또는 "구현예"에 대한 본 명세서 전체를 통한 참조는 구현예와 결부되어 기재된 특별한 특징, 형태, 조성 또는 특성이 본 발명의 하나 이상의 구현예에 포함됨을 의미한다. 따라서, 본 명세서 전체에 걸친 다양한 위치에서 표현된 "한 가지 구현예에서" 또는 "구현예"의 상황은 반드시 본 발명의 동일한 구현예를 나타내지는 않는다. 추가로, 특별한 특징, 형태, 조성, 또는 특성은 하나 이상의 구현예에서 어떠한 적합한 방법으로 조합될 수 있다.
본 발명 내 특별한 정의가 없으면 본 명세서에 사용된 모든 과학적 및 기술적인 용어는 본 발명이 속하는 기술분야에서 당업자에 의하여 통상적으로 이해되는 것과 동일한 의미를 가진다.
본 발명의 일 구현 예에 따르면, 모노머형 스트렙타비딘(monomeric streptavidin; mSA)을 코딩하는 유전자가 도입되어 형질 전환된 숙주 세포를 포함하는, 암의 진단을 위한 조성물을 제공한다.
본 발명의 다른 구현 예에 따르면, 목적하는 개체에, 상기 암의 진단을 위한 조성물을 유효량으로 투여하는 단계를 포함하는, 암의 진단을 위한 정보 제공 방법을 제공한다.
*본 발명에서 상기 "스트렙타비딘(streptavidin)"은 비오틴(biotin)에 대하여 높은 결합력을 갖는 단백질이며 상기 비오틴과의 특이적인 상호작용을 이용하여 다양한 생물학적 응용분야에 적용되어 왔다. 상기 스트렙타비딘 단백질의 아미노산 서열은 상기 서열번호 1과 같이 나타낼 수 있고, 상기 스트렙타비딘을 코딩하는 유전자는 서열번호 2로 표시되는 것일 수 있으나, 이에 제한되지는 않는다.
본 발명에서 상기 "모노머형 스트렙타비딘(monomeric streptavidin; mSA)"은 상기 스트렙타비딘이 테트라머를 형성하며 바이오틴 컨쥬게이트의 원치 않는 가교 결합을 초래할 수 있도록, 모노머로 존재하는 스트렙타비딘이다.
본 발명에서, 상기 숙주 세포는 말토오즈 결합 단백질(maltose-binding protein; MBP)을 코딩하는 유전자가 더 도입된 것일 수 있다. 상기 "말토오즈 결합 단백질(maltose-binding protein; MBP)"은 대장균의 말토오즈/말토 덱스트린 시스템의 일부로, 말토 덱스트린의 흡수와 효율적인 이화 작용을 담당하는 약 42.5kDa의 단백질이며, 서열번호 3의 아미노산 서열로 나타낼 수 있고, 상기 말토오즈 결합 단백질을 코딩하는 유전자는 서열번호 4로 표시되는 것일 수 있으나, 이에 제한되지는 않는다.
본 발명에서, 상기 숙주 세포는 상기 모노머형 스트렙타비딘을 코딩하는 유전자의 발현을 조절하는 조절 유전자가 더 도입된 것일 수 있다. 상기 "조절" 내지 "발현 조절"은 특정 유전자의 전사 및 번역이 활성화 또는 억제되는 것을 의미할 수 있다.
본 발명에서, 상기 조절 유전자는 구조적으로 DNA-의존 RNA 폴리머라제(DNA-dependent RNA polymerase) 결합 위치, 전사 개시 지점 및 전사 인자 결합 지점, 억제 및 활성 단백질 결합 지점 및 당해 기술 분야의 숙련자에게 직접적으로 혹은 간접적으로 전사량 조절에 작용할 수 있다고 알려진 뉴클레오티드의 다른 어떠한 서열을 포함되는 핵산 분획을 나타낼 수 있으나, 이에 제한되지 않는다.
본 발명에서, 상기 조절 유전자는 상기 모노머형 스트렙타비딘을 코딩하는 유전자의 개시코돈의 5' 상류에 작동가능하도록 연결될 수 있다.
본 발명에서, 상기 조절 유전자는 리보솜 결합 부위(Ribosome binding site; RBS), 5'-비 번역 영역(5'-Untransrated Region; 5'-UTR) 및 전사 인자 결합 부위(transcription factor binding site)로 이루어진 군으로부터 선택되는 적어도 하나인 것일 수 있으나, 이에 제한되는 것은 아니다.
본 발명에서, 상기 "리보솜 결합 부위(Ribosome-binding site; RBS)"는 유전자의 개시코돈의 상류에서 리보솜의 모집을 담당하여 번역을 진행시킨다. 원핵 생물의 리보솜 결합 부위는 5'-AGGAGG-3' 서열을 가지는 샤인-달가노(Shine-Dalgarno; SD) 서열을 포함한다. 상기 샤인-달가노 서열에 16S rRNA의 3' 말단이 상보적으로 결합하여 번역이 시작되는데, 상기 상보적인 서열인 CCUCCU 서열을 항-샤인-달가노(anti-Shine-Dalgarno; ASD) 서열이라고 한다.
본 발명에서, 상기 "5'-비 번역 영역(5'-Untransrated Region; 5'-UTR)"은 5' 지역에서 mRNA의 아미노산으로 번역되는 부분인 코딩 부분(coding region)의 양 쪽에 위치하는 번역이 되지 않는 부분으로, 진화 과정에서 불필요하게 버려지는 부분(junk)로 여겨졌으나, 유전자 발현 조절에 큰 역할을 하는 것으로 알려졌다.
본 발명에서, 상기 "전사 인자 결합 부위(transcription factor binding site)"는 근처의 특정 유전자를 켜거나 끄는 역할을 하는 DNA 부위이다. 상기 전사 인자 결합 부위는 상기 조절 단백질을 암호화하는 유전자의 프로모터, 인핸서(enhancer) 및 사일렌서(silencer)로 이루어진 군으로부터 선택되는 적어도 하나인 것일 수 있으나, 이에 제한되지는 않는다.
본 발명에서, 상기 조절 유전자는 재조합된 상기 벡터가 숙주 세포에 형질 전환되는 경우, 상기 숙주 세포의 주변 세포질로 모노머형 스트렙타비딘이 발현되도록 하는 것이, 상기 숙주 세포의 내부에 머물거나 상기 주변 세포질에 머물지 않고 배출되는 경우보다 발현되는 모노머형 스트렙타비딘의 활용성이 높아 바람직하다.
본 발명에서, 상기 조절 유전자는 서열번호 26 내지 92 중 어느 하나로 표시되는 것일 수 있다.
본 발명에서, 상기 조절 유전자는 총 깁스 자유 에너지 변화량(ΔGtotal)이 0 이하인 것일 수 있다. 상기 "총 깁스 자유에너지 변화량(total Gibbs free energy change; ΔGtotal)"은, 상기 모노머형 스트렙타비딘의 번역 과정에서, 상기 조절 유전자의 mRNA 전사체(transcript)가 리보솜의 30S 서브유닛 복합체 상에 결합하기 전과 후의 깁스 자유 에너지의 차이 값이다. 상기 총 깁스 자유 에너지 변화량(ΔGtotal)이 0 이하인 경우, 상기 모노머형 스트렙타비딘을 코딩하는 유전자의 전사와 번역 능력이 상승할 수 있다. 상기 총 깁스 자유 에너지 변화량(ΔGtotal)은 하기 식 1 및 식 2와 같은 방법으로 계산될 수 있다.
[식 1]
ΔGtotal = (ΔGfinal) - (ΔGinitial)
[식 2]
(ΔGfinal) - (ΔGinitial) = [(ΔGmRNA-rRNA) + (ΔGspacing) + (ΔGstacking) + (ΔGstandby) + (ΔGstart)] - (ΔGmRNA)
상기 식 1 및 식 2에서, 상기 "ΔGfinal"은 리보솜의 30S 서브유닛 복합체가 상기 조절 유전자의 mRNA 전사체 상에 결합한 후의 깁스 자유 에너지 변화량이고; 상기 "ΔGinitial"은 리보솜의 30S 서브유닛 복합체가 상기 조절 유전자의 mRNA 전사체 상에 결합하기 전의 깁스 자유 에너지 변화량이다. 또한, 상기 식 2에서, 상기 "ΔGmRNA-rRNA"는 조절 유전자의 mRNA와 리보솜의 30S 서브유닛의 복합체를 형성하는 반응이 일어날 때의 깁스 자유 에너지 변화량이고, 상기 "ΔGspacing"은 상기 조절 유전자의 mRNA 전사체에서 리보솜의 30S 서브유닛의 복합체를 형성하는 서열과 개시코돈 간의 간격(Spacing)이 최적화 되지 않음으로써 발생하는 대한 깁스 자유 에너지 패널티(penalty)이며, 상기 "ΔGstacking"은 상기 간격(Spacing)의 영역에서 쌓인 뉴클레오티드의 깁스 자유 에너지 변화량이고, 상기 "ΔGstandby"는 상기 조절 유전자의 mRNA 전사체의 대기 부위(Stanby Site)와 리보솜 간 결합 반응이 일어나는 경우 깁스 자유 에너지 패널티, 상기 "ΔGstart"는 mRNA-tRNA 복합체를 형성하는 반응이 일어날 때의 깁스 자유 에너지 변화량, 상기 "ΔGmRNA"는 상기 조절 유전자의 mRNA 전사체가 접힌 복합체 구조물을 형성할 때의 깁스 자유 에너지 변화량이다.
본 발명에서, 상기 각 깁스 자유 에너지 변화량(ΔG)은 NUPACK, ViennaRNA 또는 UNAfold와 같이, 희석된 용액에서 유전자 가닥들의 상호 작용, 농도, 염기쌍의 복잡도, 매듭 구조 등의 변수를 고려하여 계산을 수행하는 소프트웨어에 의해 계산될 수 있으나, 이에 제한되지는 않는다.
본 발명에서, 상기 조절 유전자는 모노머형 스트렙타비딘의 생산량을 극대화할 수 있도록, 번역 개시 속도(Translation Initiation Rate; TIR)가 특정 범위를 갖도록 조절된 것일 수 있다.
본 발명에서 상기 "번역 개시 속도(Translation Initiation Rate; TIR)"는 하기 식 3으로 계산될 수 있으며, 합성 생물학에서 번역 단계는 전체 단백질 생산의 속도를 제한하는 단계이므로 유전자의 발현에 중요한 인자이다.
[식 3]
TIR = exp[k{(ΔGtotal) - (ΔG1total)}]
상기 식 3에서,
TIR의 단위는 au이고;
k는 볼츠만 상수로, 0.4 내지 0.6 mol/kcal일 수 있으며;
ΔGtotal은 상기 식 1에서 정의된 바와 같고;
ΔG1total은 상기 조절 유전자가 포함되지 않은 본 발명의 벡터에서의 깁스 자유 에너지 변화량에 해당하고, 바람직하게는 상기 조절 유전자가 포함되지 않고, 나머지 서열은 동일한 벡터의 자유 에너지 변화량에 해당할 수 있으나, 이에 제한되지는 않는다. 따라서, 상기 조절 유전자가 포함되지 않을 경우에 번역 개시 속도는 1au에 해당한다.
본 발명에서, 상기 조절 유전자는 번역 개시 속도가 50 내지 45000au, 바람직하게는 900 내지 45000au가 되도록 조절된 것이 형질 전환된 균주가 상기 모노머형 스트렙타비딘을 고효율로 생산할 수 있어 바람직하다.
본 발명에서, 상기 조절 유전자의 서열 길이는 15 내지 39bp인 것일 수 있고, 바람직하게는 26 내지 31bp로 이루어 질 수 있으나, 이에 제한되지는 않는다.
본 발명에서, 상기 조절 유전자는 서열번호 5로 표시되는 유전자 서열인 "AGG"를 포함하는 것일 수 있고, 상기 조절 유전자는 서열번호 6으로 표시되는 유전자 서열인 "TAGG"를 포함하는 것일 수 있으며, 상기 조절 유전자는 서열번호 7로 표시되는 유전자 서열인 "ATAGG"을 포함하는 것일 수 있으나, 이에 제한되지는 않는다.
본 발명에서, 상기 조절 유전자는 서열번호 5 내지 7로 표시되는 유전자 서열의 3' 말단에서 개시코돈 까지의 간격(Spacing)이 6 내지 13bp, 바람직하게는 6 내지 10bp인 것일 수 있다. 상기 간격이 6 내지 13bp인 경우, 상기 mRNA 전사체에서 rRNA 복합체를 형성하는 서열과 개시코돈 간의 최적화되지 않은 간격(Spacing)에 대한 깁스 자유 에너지 패널티(ΔGspacing)가 최소화되어, 그 결과 모노머형 스트렙타비딘의 발현 수준을 높일 수 있다.
본 발명의 바람직한 일 예시에서, 상기 조절 유전자는 상기 식 1의 총 깁스 자유 에너지 변화량(ΔGtotal)이 0 이하이고, 번역 개시 속도(TIR)가 900 내지 9000au로 조절된 것으로, 서열 길이는 26 내지 31bp로 이루어진 것이며, 서열번호 5 내지 7 중 어느 하나로 표시되는 유전자 서열을 포함하고, 상기 서열번호 5 내지 7 중 어느 하나로 표시되는 유전자의 3' 말단으로부터 상기 모노머형 스트렙타비딘을 코딩하는 유전자의 개시코돈까지 간격(Spacing)이 6 내지 10bp일 수 있다.
본 발명의 바람직한 다른 일 예시에서, 상기 조절 유전자는 서열번호 32 또는 36으로 표시될 수 있다.
본 발명의 형질 전환은 벡터에 의해 이루어질 수 있다. 상기 "벡터(vector)"내지 유전자 컨스트럭트는 외래 유전자를 세포로 전달하여 발현할 수 있는 수단으로, 본 발명의 상기 벡터는 플라스미드(plasmids), 코스미드(cosmids), 인공 염색체(artificial chromosomes), 리포솜(Liposomes)과 같은 비바이러스성 벡터이거나, 레트로바이러스(retrovirus), 아데노바이러스(Adenovirus), 아데노-관련 바이러스(Adenovirus-associated virus; AAV), 파지(phage)와 같은 바이러스성 벡터일 수 있다.
본 발명에서 상기 "플라스미드(plasmids)"는 염색체와 분리되어 있고 자신의 복제기점을 소유하여 독립적으로 증식 가능한 에피솜 DNA 분자이다. 상기 플라스미드는 제한효소에 의해 재조합된 후 숙주 세포에 전달되어 벡터의 기능을 할 수 있다.
본 발명에서 상기 "코스미드(cosmids)"는 파이 파지의 점착성 말단(cohesive ends)인 cos 부위를 이용한 플라스미드로, 삽입할 수 있는 유전자의 크기가 커서 유전자 라이브러리를 만드는데 주로 사용된다.
본 발명에서 상기 "인공 염색체(artificial chromosomes)"는 벡터로 사용하기 위하여 인위적으로 구조를 변화시킨 염색체로, 박테리아 인공염색체, 효모 인공염색체, 인간 인공염색체 등이 있다.
본 발명에서 상기 "리포솜(Liposomes)"은 인위적으로 만든 1개 이상의 지질 2중층으로 되어 있는 소낭 구조물로, 세포막의 형태와 유사하고 다양한 물질을 혼입하는 능력 때문에 핵산뿐만 아니라 펩티드, 항체, 앱타머 등을 전달하는 약물 운반체 시스템이다. 상기 리포솜의 효능은 막과 성분의 특성에 따른 표적 전달 및 침투 능력에 달려 있다.
본 발명에서 상기 "레트로바이러스(retrovirus)"는 역전사(reverse transcription)를 통한 DNA 중간체를 필수로 하는 단일가닥의 양성 센스(positive-sense) RNA를 게놈으로 가지는 바이러스로, 레트로바이러스 벡터는 숙주 세포의 염색체에 삽입되어 세포분열을 하여도 바이러스 벡터가 안정적으로 유지되므로 유전자 치료에 많이 사용된다.
본 발명에서, 상기 "렌티바이러스(Lentivirus)"는 레트로바이러스의 일종으로, 숙주에 내인성인 바이러스(endogenous retrovirus; ERV)이다. 비리온 입자는 약간 다형성의 직경 80 내지 100nm 구형이고, 뉴클레오캡시드(코어)는 등척성이며, 뉴클레오티드는 동심 막대 형상 또는 원추 형상이다.
본 발명에서 상기 "아데노바이러스(Adenovirus)"는 약 36kb DNA를 가지는 바이러스로, 50가지 이상의 유전자를 가지고 있어 몇 가지의 바이러스 유전자를 발현하려는 유전자로 치환하여 벡터를 생성할 수 있다.
본 발명에서 상기 "아데노바이러스-관련 바이러스(Adenovirus-associated virus; AAV)"는 DNA 유전체가 매우 작고, 아데노바이러스가 필요한 인공위성바이러스(Satellite virus)로, 벡터로 사용할 경우 사람 염색체의 특정한 부위에 삽입되어 잠복감염(latent infection)을 일으킨다.
본 발명에서, 상기 재조합 벡터는 구성적 발현 벡터(Constitutive Expression Vector)나 유도성 발현 벡터(Inducible Expression Vector) 일 수 있고, 그 예시로 pKD13, pCP20, pMA1, pUC19, pJL, pBAD, pET, pGEX, pMAL, pALTER, pCal, pcDNA, pDUAL, pTrc, pQE, pTet, pProEX HT, pPROLar.A, pPROTet.E, pRSET, pSE280, pSE380, pSE420, pThioHis, pTriEx, pTrxFus, Split GFP Fold 'n' Glow, pACYCDuet-1, pCDF-1b, pCDFDuet-1, pCOLADuet-1, pLysS, pRSF-1b, pRSFDuet-1, pT7-FLAG, T7Select, pCMV, pBluescript, pBac, pAc, pFastBacHT, pFastBac, pAO815, pPIC, pESC, pCas9, pwtCas9-bacteria, pgRNA-bacteria 및 pGRG 플라스미드 또는 에서 선택된 1 이상의 플라스미드에서 유래된 것일 수 있으나, 이에 제한되지는 않는다.
본 발명의 일 예시에서 상기 pKD13은 약 3.4kbp로 이루어져 있고, 베타-락타마제(beta-lactamase), Tn5 네오마이신 포스포트랜스퍼라제(Tn5 neomycin phosphotransferase), 람다 종결자(lambda terminator) 및 R6K 감마 복제 원점(R6K gamma replication origin) 유전자를 포함할 수 있다.
본 발명의 일 예시에서, 상기 pCP20 플라스미드는 약 9.4kbp로 이루어져 있고, EcoRI, cat, Pstl, HindⅢ, Ci857, flp, bamHi, 베타-락타마제(beta-lactamase), mobA, mob2 및 repA101ts 유전자 부위를 포함할 수 있다.
본 발명의 일 예시에서, 상기 pMA1 플라스미드는 Microcystis aeruginosa f. aeruginosa Kutzing에서 유래하고, 약 2.3kbp로 이루어져 있으며, HincⅡ 유전자 부위를 포함하는 것일 수 있다.
본 발명의 일 예시에서, 상기 pJL 플라스미드는 빈 백본을 가지고 RNA 바이러스 기반인 것일 수 있다.
본 발명의 일 예시에서, 상기 pBAD, pCMV 및 pCMV 플라스미드는 포유류 숙주세포에서 발현되고 CMV, 프로모터를 이용하며, 암피실린내성을 가지는 것일 수 있다.
본 발명의 일 예시에서, 상기 pET, pBluescript, pCal 및 pcDNA 플라스미드는 박테리아 숙주세포에서 발현되고 T7 또는 Lac 프로모터를 이용하며, 암피실린 내성을 가지는 것일 수 있다.
본 발명의 일 예시에서, 상기 pMAL 및 pGEX 플라스미드는 박테리아 숙주세포에서 발현되고 Tac 프로모터를 이용하며, 암피실린 내성을 가지는 것일 수 있다.
본 발명의 일 예시에서, 상기 pALTER 플라스미드는 박테리아 숙주세포에서 발현되고 T7 프로모터를 이용하며, 테트라사이클린 내성을 가지는 것일 수 있다.
본 발명의 일 예시에서, 상기 pDUAL 플라스미드는 박테리아 숙주세포에서 발현되고 T7 또는 Lac 프로모터를 이용하며, 카나마이신 내성을 가지는 것일 수 있다.
본 발명의 일 예시에서, 상기 pTrc 플라스미드는 박테리아 숙주세포에서 발현되고 trc 프로모터를 이용하며, 암피실린 내성을 가지는 것일 수 있다.
본 발명의 일 예시에서, 상기 pUC19 플라스미드는 박테리아 숙주세포에서 발현되고, 약 2.6kbp의 원형 이중 가닥 DNA로 이루어져 있으며, pUC18과 MCS 영역이 반대인 벡터이다. 상기 pU19 벡터는 형질 전환을 위하여 가장 널리 사용되는데, 상기 pU19에 의해 외래 DNA가 도입된 숙주 세포는 대조군에 비하여 성장 배지 속 콜로니의 색상이 달라지므로, 구별이 가능하다.
본 발명의 일 예시에서, 상기 pQE 플라스미드는 T5-lac 프로모터를 이용하고, 암피실린 내성을 가지는 것일 수 있다.
본 발명의 일 예시에서, 상기 pTet 플라스미드는 tet 오페론으로부터의 조절 서열의 제어 하에 CMV 프로모터를 포함하고 있어, 트랜스 활성화제인 pTet-tTAk와 공동 형질 감염될 때 독시사이클린이 없는 경우에만 단백질을 발하는 것일 수 있다.
본 발명의 일 예시에서, 상기 pCas9, pwtCas9-bacteria 및 pgRNA-bacteria 플라스미드는 CRISPR 기술을 사용하여, Cas9 뉴클레아제 gRNA을 발현시키기 위한 것일 수 있다.
본 발명에서, 상기 숙주 세포를 형질 전환시키는 방법은 당업계의 통상적인 도입 방법에 따라 수행될 수 있고, 구체적인 방법을 특별히 제한하지는 않으나, 예를 들면, 박테리아의 형질 전환 방법, CaCl2 침전법, CaCl2 방법에 환원물질인 DMSO(Dimethyl sulfoxide)를 사용함으로써 효율을 높인 Hanahan 방법, 전기천공법(Electroporation), 인산칼슘 침전법, 원형질 융합법, 실리콘 카바이드 섬유를 이용한 교반법, 아그로 박테리아 매개된 형질 전환법, PEG를 이용한 형질 전환법, 덱스트란 설페이트, 리포펙타민 및 건조/억제 매개된 형질 전환 방법 등을 사용할 수 있다.
본 발명에서, 상기 숙주 세포를 암이 발병한 개체에 투여하는 경우 암 조직에서만 모노머형 스트렙타비딘을 발현할 수 있는 효과가 있다. 따라서, 본 발명의 상기 숙주 세포는 암이 발병한 개체에 투여하였을 때에 암 조직에 비하여 정상 조직에서 생존력이 낮은 것이, 정상 조직에는 감염이 없고, 암 조직에서만 모노머형 스트렙타비딘을 발현할 수 있어 바람직하다. 여기서, 상기 정상 조직은 폐, 간 및 비장으로 이루어진 군에서 선택되는 장기의 조직일 수 있으나, 이에 제한되지 않는다.
본 발명에서, 상기 숙주 세포는 박테리아, 균류, 포유 동물, 식물 및 곤충으로 이루어진 군으로부터 선택되는 어느 하나 이상의 세포인 것일 수 있고, 예를 들면 대장균, 스트렙토미세스 또는 살모넬라(Salmonella) 속 균주 등의 박테리아 세포, 효모 세포, 피치아 파스 토리스 등의 균류 세포; 드로조필라, 스포도프테라 Sf9 세포 등의 곤충 세포, CHO(중국 햄스터 난소 세포, Chinese hamster ovary cells), SP2/0(생쥐 골수종), 인간 림프아구(Human lymphoblastoid), COS, NSO(생쥐 골수종), 293T 세포, 보우 멜라노마 세포, HT-1080 세포, BHK 세포(베이비 햄스터 신장세포, Baby Hamster Kidney cells), HEK 세포(인간 배아신장 세포, Human Embryonic Kidney cells) 또는 PERC.6 세포(인간 망막 세포)와 같은 동물 세포, 및 식물 세포로 구성된 군으로부터 선택되는 적어도 하나일 수 있으나, 이에 제한되는 것은 아니다.
본 발명에서, 상기 숙주 세포는 박테리아 세포일 수 있고, 바람직하게는 혐기성 균주인 것일 수 있다. 상기한 숙주 세포를 암 진단, 예방 및 치료의 목적으로 인체에 주입한 경우에, 혈관 형성이 불완전하여 산소가 결핍된 환경인 암 조직의 내부를 표적하기 때문에, 이와 같은 균주 내에 실시간으로 이미징이 가능한 리포터 단백질과 항암 단백질이 동시에 균형적으로 발현될 수 있는 재조합 벡터가 도입되어 있는 경우 암을 매우 효과적으로 진단과 동시에 예방 및 치료가 가능할 수 있도록 한다.
본 발명의 일 예시에서, 상기 박테리아는 살모넬라 속 균주, 클로스트리듐(Clostridium) 속 균주, 비피도박테리움(Bifidobacterium) 속 균주 및 대장균 속 균주로 구성된 군으로부터 선택되는 적어도 하나일 수 있으며, 보다 바람직하게는 살모넬라 티피뮤리움(Salmonella typhimurium), 살모넬라 콜레라수스(Salmonella choleraesuis) 및 살모넬라 엔테리티디스(Salmonella enteritidis)로 구성된 군으로부터 선택되는 적어도 하나인 것일 수 있고, 더욱 바람직하게는 살모넬라 티피뮤리움일 수 있으나, 이에 제한되는 것은 아니다.
본 발명에서 상기 "살모넬라 티피무륨(Salmonella typhimurium)"은 살모넬라(Salmonella) 속의 장티푸스를 일으키는 원인균이다. 상기 살모넬라 티피무륨은 막대기 모양의 간균으로 편모가 있고 그람 음성이다. 상기 살모넬라 티피무륨은 열에 약하여 60℃에서 20분만에 사멸하며, 가축, 야생 동물, 보균자 등이나 우유, 계란 등에 의해 1차 오염되고 오염된 생육 등에서 2차 감염을 받기 쉬운 샐러드 등도 원인이 되어 식중독의 일종인 살모넬라증(Salmonellosis)을 유발할 수 있다.
본 발명에서 상기 "살모넬라 콜레라수이스(Salmonella choleraesuis)"는 살모넬라(Salmonella)속의 돼지 콜레라균으로 잘 알려져 있는 균으로 사람과 동물 모두에게 감염되는 균이다. 상기 살모넬라 콜레라수이스는 살모넬라에 의한 급성패혈증의 주된 원인균이다. 이 균은 주모(周毛)를 가지고 있어 운동성이 있는 그람 음성의 통성 혐기성 간균이다. 유당 분해력이 없고, 인돌을 형성하지 않으며, 황화수소를 생성하지 않아 대장균과는 구별된다. 발육최적온도는 35~37℃이고, 증식가능 온도범위는 10~43℃이며, 60℃에서 20분간 가열로 사멸한다. 최적 pH는 7.2~7.4이고, 크기는 0.5~0.8Х3~4μm이다.
본 발명에서 상기 "살모넬라 엔테리티디스(Salmonella enteritidis)"는 살모넬라(Salmonella) 속의 세균성 감염형 식중독의 원인균으로 장염균이라고도 한다. 상기 살모넬라 엔테리티디스는 살모넬라의 대표적인 균으로 모든 동물에서 감염을 일으킬 수 있고 숙주 적응력이 매우 높다. 주모(周毛)를 가지고 있어 운동성이 있는 그람 음성의 통성 혐기성 간균이다. 유당 분해력이 없고, 인돌을 형성하지 않으며, 황화수소를 생성하지 않아 대장균과는 구별된다. 발육 최적 온도는 35~37℃이고 증식이 가능한 온도 범위는 10~43℃이며, 60℃에서 20분간 가열로 사멸한다. 최적 pH는 7.2~7.4이고, 크기는 0.5~0.8Х3~4μm이다.
본 발명에서 상기 "살모넬라 인판티스(Salmonella infantis)"는 계란 또는 가금육류에 의해 감염되는 균주이고, 파라티푸스 균(Salmonella paratyphi) 및 장티푸스 균(Salmonella typhi)은 장티푸스의 원인균주이다.
또한, 본 발명에서, 상기 박테리아는 개체에 투여 시 독성 및 다른 부작용 등을 감소시킬 수 있도록 약독화된 것일 수 있다.
본 발명의 일 예시에서, 상기 박테리아는 그에서 발현되는 aroA, aroC, aroD, aroE, Rpur, htrA, ompR, ompF, ompC, galE, cya, crp, cyp, phoP, phoQ, rfaY, dksA, hupA, sipC, clpB, clpP, clpX, pab, nadA, pncB, pmi, rpsL, hemA, rfc, poxA, galU, cdt, pur, ssa, guaA, guaB, fliD, flgK, flgL, relA, spoA 및 spoT로 이루어진 군으로부터 선택되는 적어도 하나를 코딩하는 유전자가 변형되어 약독화된 것일 수 있다.
본 발명의 다른 일 예시에서, 상기 박테리아는 구아노신 다인산 합성능이 결여되어 약독화된 것일 수 있다. 상기 구아노신 다인산은 구아노신-5-이인산-3-이인산(ppGpp)일 수 있고, 상기 숙주 세포는, 구아노신-5-이인산-3-이인산(ppGpp)을 가수분해하는 RelA 또는 구아노신-5-이인산-3-이인산(ppGpp)을 합성하는 spoT 코딩하는 유전자가 변형되어 상기 구아노신-5-이인산-3-이인산(ppGpp)의 합성능이 결여된 것일 수 있으나, 이에 제한되는 것은 아니다.
본 발명에서, 상기 박테리아에 유전자에 변형을 가하는 방법은 당업계에 공지된 다양한 유전자의 결실 또는 파괴하는 방법에 의해 수행될 수 있고, 예를 들면, 상기 결실 및 파괴 방법은 상동성 재조합, 화학적 변이유발, 조사 변이유발 또는 트랜스포존 변이유발 등과 같은 방법에 의해 수행될 수 있으나, 이에 제한되는 것은 아니다.
본 발명의 조성물은 비오틴화된 화합물을 더 포함할 수 있고, 이때 상기 숙주 세포에서 발현되는 모노머형 스트렙타비딘은 비오틴-결합성 단백질로서, 비오틴과 상호 작용할 수 있는 결합 부위를 포함할 수 있다.
본 발명에서, 상기 비오틴화된 화합물은 비오틴, 바람직하게는 D-비오틴과 반응하도록 적어도 하나의 아민기를 포함하는 화합물에, D-비오틴의 N-히드록시숙신이미딜 에스테르 (NHS-비오틴) 와 같은 일반적인 비오틴화된 시약을 이용하여 비오틴기를 갖도록 개질된 것일 수 있다.
본 발명에서, 상기 비오틴화된 화합물은 상기 숙주 세포에서 발현되는 모노머형 스트렙타비딘을 예비 표적화 할 수 있고, 이에 따라 상기 숙주 세포, 또는 상기 숙주 세포로부터 발현되는 모노머형 스트렙타비딘과, 비오틴화된 화합물을 체내에 투여하는 경우, 체내에서 높은 효율로 화합물-비오틴-스트렙타비딘 복합체가 형성될 수 있다.
본 발명에서, 상기 비오틴화된 화합물은 비오틴화된 조영제일 수 있다. 여기서, 상기 조영제는 방사성 핵종, 형광 표지, 효소 표지, 화학 발광 마커, 금 제제 및 자성 제제로 이루어진 군으로부터 선택되는 적어도 하나인 것일 수 있으나, 알파선, 감마선, 양전자(Positron), X-선, 자외선, 가시광선, 적외선, 초음파(ultrasonic wave) 및 자기 공명(magnetic resonance)에서 선택된 하나 이상의 신호를 발생하거나 증폭시키는 것이라면 어떤 것이든 제한되지는 않는다.
본 발명에서, 상기 방사성 동위원소 내지 방사성 핵종은 C-11, F-18, Cu-64, Ga-68, Zr-89, Y-90, Tc-99, In-111, I-123, I-124, I-125, I-131, Lu-177, Ho-166 및 Sm-153으로 이루어진 군에서 선택된 어느 하나 이상인 것일 수 있으나, 이에 제한되지는 않는다.
본 발명에서, 상기 방사성 동위원소 내지 방사성 핵종을 검출할 수 있는 수단은, 양전자 단층촬영(PET) 또는 뇌 단일 광자 단층촬영(SPECT) 일 수 있으나, 이에 제한되지는 않는다. 상기 양전자 단층촬영(PET)은 양전자를 방출하는 방사성 동위원소를 결합한 약물을 체내에 주입하여, 체내에서 발생하는 양전자를 측정해 실시간으로 영상을 구성하는 방법이고, 상기 뇌 단일 광자 단층촬영(SPECT)은 환자가 혈관으로 동위원소를 주입하여 뇌혈류의 상태를 보여주는 검사이다.
본 발명에서, 상기 형광 표지는 플루오레세인, 로다민, 로돌, 로사민 및 이들의 유도체를 비제한적으로 포함하는 피페라진, 크산텐, 쿠마린, 아크리딘, 푸란, 인돌, 퀴놀린, 시아닌, 벤조푸란, 퀴나졸리논, 벤자졸, 붕소-디피로메텐, 및 이들의 유도체로 이루어진 군으로부터 선택된 어느 하나 이상일 수 있으나, 이에 제한되지는 않는다.
본 발명에서, 상기 효소 표지는 양고추냉이 퍼옥시다제, 루시퍼라제 및 알칼리성 포스파타제로 이루어진 군에서 선택된 어느 하나 이상인 것일 수 있으나, 이에 제한되지는 않는다.
본 발명에서, 상기 암은 흑색종, 나팔관암, 뇌암, 소장암, 식도암, 임파선암, 담낭암, 혈액암, 갑상선암, 내분비선암, 구강암, 간암, 담도암, 대장암, 직장암, 자궁경부암, 난소암, 신장암, 위암, 십이지장암, 전립선암, 유방암, 뇌종양, 폐암, 갑상선 미분화암, 자궁암, 결장암, 방광암, 요관암, 췌장암, 뼈/연부조직 육종, 피부암, 비호지킨 림프종, 호지킨 림프종, 다발성 골수종, 백혈병, 골수이형성증후군, 급성 림프모구성 백혈병, 급성 골수성 백혈병, 만성 림프구성 백혈병, 만성 골수성 백혈병 및 고립성 골수종으로 구성되는 군으로부터 선택되는 적어도 하나 일 수 있고, 바람직하게는 흑색종, 나팔관암, 뇌암, 소장암, 식도암, 임파선암, 담낭암, 갑상선암, 내분비선암, 구강암, 간암, 담도암, 대장암, 직장암, 자궁경부암, 난소암, 신장암, 위암, 십이지장암, 전립선암, 유방암, 뇌종양, 폐암, 갑상선 미분화암, 자궁암, 결장암, 방광암, 요관암, 췌장암, 뼈/연부조직 육종, 피부암 및 골수종으로 구성되는 군으로부터 선택되는 적어도 하나 일 수 있으나, 이에 제한되지는 않는다.
본 발명에서 상기 "진단"은 특정 질병 또는 질환에 대한 대상(subject)의 감수성(susceptibility)을 판정하는 것, 대상이 특정 질병 또는 질환을 현재 가지고 있는지 여부를 판정하는 것, 특정 질병 또는 질환에 걸린 대상의 예후(prognosis)(예컨대, 전-전이성 또는 전이성 암 상태의 동정, 암의 단계 결정 또는 치료에 대한 암의 반응성 결정)를 판정하는 것, 또는 테라메트릭스(therametrics)(예컨대, 치료 효능에 대한 정보를 제공하기 위하여 객체의 상태를 모니터링하는 것)을 포함한다. 본 발명의 목적상, 상기 진단은 상기한 암의 발병 여부 또는 암 조직의 크기를 확인하는 것이다.
본 발명에서 상기 "개체"란, 암의 진단, 예방 또는 치료가 필요한 개체로서, 포유동물 및 비-포유동물을 모두 포함할 수 있다. 여기서, 상기 포유동물의 예로는 인간, 비-인간 영장류, 예컨대 침팬지, 다른 유인원 또는 원숭이 종; 축산 동물, 예컨대 소, 말, 양, 염소, 돼지; 사육 동물, 예컨대 토끼, 개 또는 고양이; 실험 동물, 예를 들어 설치류, 예컨대 래트, 마우스 또는 기니아 피그 등을 포함할 수 있으나, 이에 제한되는 것은 아니다. 또한, 본 발명에서 상기 비-포유동물의 예로는 조류 또는 어류 등을 포함할 수 있으나, 이에 제한되는 것은 아니다.
본 발명에서 상기 "투여"란, 임의의 적절한 방법으로 개체에게 본 발명의 유효성분을 도입하는 과정을 의미하는 것으로서, 상기와 같이 투여되는 화합물의 제제는 특별히 제한하지 않으며, 고체 형태의 제제, 액체 형태의 제제 또는 흡인용 에어로졸 제제로 투여될 수 있으며, 사용하기 바로 전에 경구 또는 비경구 투여용 액체 형태 제제로 전환되도록 의도되는 고체 형태 제제로 투여될 수 있고, 예를 들면, 산제, 과립제, 캡슐, 정제, 수성 현탁액 등의 경구형 제형, 외용제, 좌제 및 멸균 주사용액의 형태로 제형화하여 투여될 수 있으나, 이에 제한되는 것은 아니다.
또한, 본 발명에서 상기 투여 시 본 발명의 화합물과 함께 약학적으로 허용 가능한 담체를 추가로 투여할 수 있다. 여기서, 상기 약학적으로 허용되는 담체는 경구 투여 시에는 결합제, 활탁제, 붕해제, 부형제, 가용화제, 분산제, 안정화제, 현탁화제, 색소, 향료 등을 사용할 수 있으며, 주사제의 경우에는 완충제, 보존제, 무통화제, 가용화제, 등장제, 안정화제 등을 혼합하여 사용할 수 있으며, 국소투여용의 경우에는 기제, 부형제, 윤활제, 보존제 등을 사용할 수 있다. 본 발명의 화합물의 제형은 상술한 바와 같은 약학적으로 허용되는 담체와 혼합하여 다양하게 제조될 수 있다. 예를 들어, 경구 투여시에는 정제, 트로키, 캡슐, 엘릭서(elixir), 서스펜션, 시럽, 웨이퍼 등의 형태로 제조할 수 있으며, 주사제의 경우에는 단위 투약 앰플 또는 다수회 투약 형태로 제조할 수 있다. 기타, 용액, 현탁액, 정제, 캡슐, 서방형 제제 등으로 제형할 수 있다.
한편, 제제화에 적합한 담체, 부형제 및 희석제의 예로는, 락토즈, 덱스트로즈, 수크로즈, 솔비톨, 만니톨, 자일리톨, 에리스리톨, 말디톨, 전분, 아카시아 고무, 알지네이트, 젤라틴, 칼슘 포스페이트, 칼슘 실리케이트, 셀룰로즈, 메틸 셀룰로즈, 미정질 셀룰로즈, 폴리비닐피롤리돈, 물, 메틸하이드록시벤조에이트, 프로필하이드록시벤조에이트, 탈크, 마그네슘 스테아레이트 또는 광물유 등이 사용될 수 있다. 또한, 충진제, 항응집제, 윤활제, 습윤제, 향료, 유화제, 방부제 등을 추가로 포함할 수 있다.
본 발명에 따른 화합물의 투여 경로는 이들로 한정되는 것은 아니지만 구강, 정맥 내, 근육 내, 동맥 내, 골수 내, 경막 내, 심장 내, 경피, 피하, 복강 내, 비강 내, 장관, 국소, 설하 또는 직장이 포함된다. 경구 또는 비경구 투하가 바람직하다.
본 발명에서, "비경구"는 피하, 피내, 정맥내, 근육내, 관절내, 활액낭내, 흉골내, 경막내, 병소내 및 두개골내 주사 또는 주입기술을 포함한다. 본 발명의 조성물은 또한 직장 투여를 위한 좌제의 형태로 투여될 수 있다.
본 발명에서, "유효량"은 바람직한 생물학적 결과를 제공하기 위한 작용제의 충분한 양을 지칭한다. 상기 결과는 질환의 징후, 증상 또는 원인의 감소 및/또는 완화, 또는 생물계의 임의의 다른 바람직한 변화일 수 있다. 예를 들어, 예방 또는 치료 용도를 위한 "유효량"은 질환에서 임상적으로 유의한 감소를 제공하는데 요구되는, 본 발명에 개시된 화합물의 양이다. 임의의 개별적인 경우에서 적절한 "효과적인" 양은 일상적인 실험을 사용하여 당업자에 의해 결정될 수 있다. 따라서, 표현 "유효량"은 일반적으로 활성 물질이 예방 또는 치료 효과를 갖는 양을 지칭한다. 본 발명의 경우에, 활성 물질은, 암의 예방, 개선 또는 치료제이다.
본 발명의 화합물은 사용된 특정 화합물의 활성, 연령, 체중, 일반적인 건강, 성별, 정식, 투여 시간, 투여 경로, 배출율, 배합 및 예방 또는 치료될 특정 질환의 중증을 포함한 여러 요인에 따라 다양하게 변할 수 있고, 상기 화합물의 투여량은 환자의 상태, 체중, 질병의 정도, 약물 형태, 투여 경로 및 기간에 따라 다르지만 당업자에 의해 적절하게 선택될 수 있고, 1일 0.0001 내지 100mg/kg 또는 0.001 내지 100mg/kg으로 투여할 수 있다. 투여는 하루에 한번 투여할 수도 있고, 수회 나누어 투여할 수도 있다. 상기 투여량은 어떠한 면으로든 본 발명의 범위를 한정하는 것은 아니다. 본 발명에 따른 화합물은 환제, 당의정, 캡슐, 액제, 겔, 시럽, 슬러리, 현탁제로 제형될 수 있다.
본 발명의 조성물은 단독으로, 또는 수술, 방사선 치료, 호르몬 치료, 화학 치료 및 생물학적 반응 조절제를 사용하는 방법들과 병용하여 사용할 수 있다.
본 발명의 또 다른 구현 예에 따르면, 모노머형 스트렙타비딘을 발현하는 숙주 세포가 투여된 목적하는 개체에서, 상기 숙주 세포를 촬영 수단으로 확인하는 단계를 포함하는, 암의 진단을 위한 정보 제공 방법을 제공한다.
본 발명에서 상기 목적하는 개체란, 암이 발병하였거나 발병 가능성이 높은 개체로서, 인간 또는 인간을 제외한 동물을 모두 포함할 수 있다. 여기서, 인간을 제외한 동물의 예로는 비-인간 영장류, 예컨대 침팬지, 다른 유인원 또는 원숭이 종; 축산 동물, 예컨대 소, 말, 양, 염소, 돼지; 사육 동물, 예컨대 토끼, 개 또는 고양이; 실험 동물, 예를 들어 설치류, 예컨대 래트, 마우스 또는 기니아 피그; 조류; 또는 어류를 포함할 수 있으나, 이에 제한되는 것은 아니다.
본 발명에서, 상기 목적하는 개체는 비오틴화된 화합물 또한 투여된 것일 수 있다. 본 발명에서 상기 비오틴화된 화합물은 모노머형 스트렙타비딘이 발현되고, 암 세포에 존재하는 상기 숙주 세포에 결합하여, 암의 크기 및 위치를 확인할 수 있는 효과를 가진다.
본 발명에서 상기 촬영 수단은, 알파선, 감마선, 양전자(Positron), X-선, 자외선, 가시광선, 적외선, 초음파(ultrasonic wave) 및 자기 공명(magnetic resonance)에서 선택된 하나 이상의 신호를 이용한 것일 수 있으나, 상기 신호는 목적하는 개체에 비침습적이거나 낮은 침습성을 가지고, 상기 비오틴화된 화합물에 의해 발생되거나 증폭되는 것이라면 제한없이 포함될 수 있다.
본 발명에서, 상기 확인하는 단계 시 상기 비오틴화된 화합물에 의해 발생되거나 증폭되는 상기 신호의 여부, 범위 또는 크기를 상기 촬영 수단에 의해 측정할 수 있다.
또한, 본 발명에서, 상기 확인하는 단계는 1회 또는 복수 회에 걸쳐 수행될 수 있다. 본 발명에서는 특정 시점을 기준으로 복수 회에 걸쳐 확인하는 단계를 수행할 수 있고, 혹은 소정의 기간에 걸쳐 복수 회로 확인하는 단계를 수행함에 따라 암의 발병 가능성의 진단 뿐만 아니라 암 환자의 예후 또한 예측할 수 있다.
본 발명의 상기 확인하는 단계에서 비오틴화된 화합물에 의해 발생된 신호가 검출되는 경우, 암이 발병하였거나 발병 가능성이 높은 것으로 예측할 수 있다.
또한, 본 발명의 확인하는 단계에서 비오틴화된 화합물에 의해 발생된 신호를 검출함으로써 암의 예후 또한 예측할 수 있다.
본 발명의 정보 제공 방법에서, 상기 숙주 세포, 암, 진단 및 비오틴화된 화합물에 관한 기재는 앞서 기재된 바와 중복되어 명세서의 과도한 복잡을 피하기 위하여 이하 그 자세한 기재를 생략한다.
본 발명의 또 다른 구현 예에 따르면, 모노머형 스트렙타비딘(monomeric streptavidin; mSA)을 코딩하는 유전자가 도입되어 형질 전환된 숙주 세포를 포함하는, 암의 예방 또는 치료를 위한 약학적 조성물을 제공한다.
본 발명의 또 다른 구현 예에 따르면, 목적하는 개체에 본 발명에 따른 숙주 세포를 포함하는 상기 약학적 조성물을 유효량으로 투여하는 단계를 포함하는, 암의 예방 또는 치료 방법을 제공한다.
본 발명에서, 상기 숙주 세포는 말토오즈 결합 단백질(maltose-binding protein; MBP)을 코딩하는 유전자가 더 도입된 것일 수 있다.
본 발명에서, 상기 숙주 세포는 상기 모노머형 스트렙타비딘을 코딩하는 유전자의 발현을 조절하는 조절 유전자가 더 도입된 것일 수 있다.
본 발명에서, 상기 조성물은 비오틴화된 화합물을 더 포함하는 것일 수 있다.
본 발명에서, 상기 비오틴화된 화합물은 비오틴화된 암 치료 물질일 수 있다. 여기서, 상기 암 치료 물질은 항암제로서, 대사길항제, 알킬화제, 토포아이소머라제 길항제, 미세소관 길항제, 항암 항생제, 식물유래 알칼로이드, 항체 항암제, 분자표적 항암제, 면역 항암제, 유전자 발현 억제제, ROS-유발 전구약물, 압타머 및 방사성 치료제로 이루어진 군으로부터 선택되는 어느 하나 이상일 수 있다.
본 발명에서 상기 항암제는 탁솔, 나이트로젠 머스타드, 이마티닙, 옥살리플라틴, 리툭시맙, 엘로티닙, 트라스투주맙, 제피티닙, 보르테조밉, 수니티닙, 카보플라틴, 소라페닙, 베바시주맙, 시스플라틴, 세툭시맙, 비스쿰알붐, 아스파라기나제, 트레티노인, 하이드록시카바마이드, 다사티닙, 에스트라머스틴, 겜투주맵오조가마이신, 이브리투모맙튜세탄, 헵타플라틴, 메칠아미노레불린산, 암사크린, 알렘투주맙, 프로카르바진, 알프로스타딜, 질산홀뮴 키토산, 젬시타빈, 독시플루리딘, 페메트렉세드, 테가푸르, 카페시타빈, 기메라실, 오테라실, 아자시티딘, 메토트렉세이트, 우라실, 시타라빈, 플루오로우라실, 플루다라빈, 에노시타빈, 데시타빈, 머캅토푸린, 티오구아닌, 클라드리빈, 카르모퍼, 랄티트렉세드, 도세탁셀, 파클리탁셀, SBT-1214, 스콰모신(Squamocin), 불라타신(bullatacin) 이리노테칸, 벨로테칸, 토포테칸, 비노렐빈, 에토포시드, 빈크리스틴, 빈블라스틴, 테니포시드, 독소루비신, 이다루비신, 에피루비신, 미톡산트론, 미토마이신, 블레오마이신, 다우노루비신, 닥티노마이신, 피라루비신, 아클라루비신, 페플로마이신, 테모졸로마이드, 부설판, 이포스파미드, 사이클로포스파미드, 멜파란, 알트레트민, 다카바진, 치오테파, 니무스틴, 클로람부실, 미토락톨, 로무스틴, 카르무스틴, 이매티닙, 게피티닙, 에르토티닙, 트리스투주맙, 로실레티닙, 네시투무맙, 에버롤리무스, 라무시루맙, 다코미티닙, 포레티닙, 팸브롤리주맙, 이필리무맙, 니볼루맙, 다브라페닙, 벨리파립, 세리티닙, 카무스틴, 시클로포스파미드, 이포스파마이드, 익사베필론, 멜팔란, 메르캅토푸린, 미토산트론, 티에스원, 레이저티닙, 부팔린, 트리아핀, 할러데이 접합(HJ) 억제제 펩타이드 2로 이루어진 군으로부터 선택되는 어느 하나 이상 일 수 있고, 바람직하게는 플루오로우라실, 독소루비신, 젬시타빈, 레이저티닙, 파클리탁셀, SBT-1214, 스콰모신, 불라타신, 부팔린, 트리아핀 및 할러데이 접합(HJ) 억제제 펩타이드 2로 이루어진 군으로부터 선택되는 어느 하나 이상 일 수 있으나, 이에 제한되지는 않는다.
본 발명의 상기 유전자의 발현 억제제는, 전사 억제제(repressor) 또는 단백질 활성 길항제(antagonist) 일 수 있다. 상기 전사 억제제는 전사의 개시를 억제하거나, 전사물의 분해를 유도하는 물질일 수 있다.
본 발명에서 상기 전사 억제제는 안티센스 올리고뉴클레오티드, 작은 간섭 RNA(small interference RNA; siRNA), 작은 헤어핀 RNA(small hairpin RNA, short hairpin RNA; shRNA), 마이크로 RNA(microRNA; miRNA) 또는 이들의 조합인 것일 수 있으나, 이에 제한되지는 않는다.
본 발명에서 상기 안티센스 올리고뉴클레오티드는 특정 mRNA의 서열에 상보적인 핵산 서열을 함유하고 있는 DNA 또는 RNA 또는 이들의 유도체를 말하고, mRNA 내의 상보적인 서열에 결합하여 mRNA의 단백질로의 번역을 저해하는 작용을 할 수 있다.
본 발명에서 상기 작은 간섭 RNA는 RNA 방해 또는 유전자 사일런싱을 매개하여 표적 유전자의 발현을 억제하는 핵산이다. 상기 작은 간섭 RNA는 견고한 헤어핀 턴을 만드는 RNA를 말하고, RNA 간섭을 통해 유전자 발현을 사일런스시키는 데 이용될 수 있다.
본 발명에서, 상기 마이크로 RNA는 21 내지 25 뉴클레오티드의 단일가닥 RNA 분자로서 mRNA의 3'-UTR(untranslation region)에 결합하여 진핵생물의 유전자 발현을 제어할 수 있다.
본 발명에서, 상기 단백질 활성 길항제(antagonist)는 단백질의 활성을 감소시키는 물질로, 상기 활성 길항제는 천연 추출물, 화학 물질, 또는 이들의 조합일 수 있다.
본 발명에서, 상기 할러데이 접합(HJ) 억제제 펩타이드 2는 서열번호 38의 아미노산 서열로 나타낼 수 있으나 이에 제한되지는 않는다.
본 발명에서, 상기 면역 항암제는 항-PD-1/PD-L1 면역 항암제일 수 있고, 상기 항-PD-1/PD-L1 면역 항암제는 니볼루맙 또는 펨브로리주맙 일 수 있으나, 세포예정사와 관련된 PD-1 또는 PD-L1과 관련된 것이라면 제한없이 포함될 수 있다.
본 발명에서, 상기 압타머는 소정의 표적 분자에 대한 결합 활성을 갖는 단일 가닥 올리고 뉴클레오티드로서, 표적 분자에 결합함으로써 표적 분자의 활성을 저해할 수 있다. 상기 압타머는 그 염기 서열에 따라 다양한 3차원 구조를 가질 수 있으며, 항원-항체 반응과 같이 특정 물질에 대하여 높은 친화력을 가질 수 있다. 상기 압타머는 RNA, DNA, 변형된(Modified) 핵산 또는 이들의 혼합물일 수 있으며, 그 형태가 직쇄상 또는 환상(環狀)일 수 있다.
본 발명에서, 상기 방사성 치료제는 알파선 또는 양전자를 방출하는 것일 수 있고, 그 예시로 Cu-67, Y-90, I-131, Lu-177, At-211, Ra-223 및 AC-225로 이루어진 군으로부터 선택되는 어느 하나 이상 일 수 있으나, 이에 제한되지는 않는다.
본 발명의 상기 "예방"이란, 본 발명의 상기 조성물을 이용하여 상기 암에 의해 발생되는 증상을 차단하거나, 그 증상을 억제 또는 지연시키는 행위라면 제한없이 포함될 수 있다.
본 발명의 상기 "치료"란, 본 발명의 상기 조성물을 이용하여 상기 암에 의해 발생된 증상이 호전되거나 이롭게 되는 행위라면 제한 없이 포함될 수 있다.
본 발명의 약학적 조성물에서, 상기 말토오즈 결합 단백질, 조절 유전자, 총 깁스 자유 에너지 변화량(ΔGtotal), 번역 개시 속도, 숙주 세포, 형질 전환, 암, 개체 및 투여에 관한 기재는 앞서 기재된 바와 중복되어 명세서의 과도한 복잡을 피하기 위하여 이하 그 자세한 기재를 생략한다.
본 발명에 따라 모노머형 스트렙타비딘을 발현하는 숙주 세포를 암이 발병한 개체에 투여하는 경우, 상기 숙주 세포로부터 발현되는 모노머형 스트렙타비딘은 체내에서 그 기능성을 유지할 수 있다. 따라서, 암을 타겟으로 예비 표적한 숙주 세포와 함께 암의 진단, 예방 또는 치료용 비오틴화된(biotinylated) 약물을 함께 투여하게 되면 상기 비오틴화된 약물은 모노머형 스트렙타비딘에 결합하여 암 조직에만 선택적으로 작용할 수 있고, 이를 통해 암의 발병 여부를 정확히 진단하거나 암을 표적으로 하는 예방 또는 치료가 가능한 장점이 있다.
도 1은 실험예 1에서 mSA 유전자를 단독으로 형질 도입시킨 플라스미드의 발현 결과를 나타낸 것이다.
도 2은 실험예 2에서 MBP-mSA 유전자 발현 결과를 나타낸 것이다.
도 3은 실험예 2에서 MBP-mSA 유전자 발현 및 활성 확인을 위한 웨스턴 블롯 결과를 나타낸 것이다.
도 4는 실험예 2에서 재조합 균주의 비오틴 결합을 분석한 결과를 그래프로 나타낸 것이다.
도 5는 실험예 2에서 재조합 균주의 비오틴 결합을 공초점 현미경을 통해 이미지로 나타낸 것이다.
도 6은 실험예 2에서 재조합 균주의 비오틴 결합을 공초점 현미경을 통해 이미지로 나타낸 것이다.
도 7은 실험예 3에서 MBP-mSA 유전자 발현 결과를 나타낸 것이다.
도 8은 실험예 3에서 MBP-mSA 유전자 발현 및 활성 확인을 위한 웨스턴 블롯 결과를 나타낸 것이다.
도 9는 실험예 4에서 MBP-mSA 유전자 발현 확인을 위한 웨스턴 블롯 결과를 나타낸 것이다.
도 10은 실험예 4에서 MBP-mSA 유전자 발현 비교를 위한 웨스턴 블롯 결과를 나타낸 것이다.
도 11은 실험예 4에서 재조합 균주의 비오틴 결합을 분석한 결과를 그래프로 나타낸 것이다.
도 12는 실험예 4에서 재조합 균주의 비오틴 결합을 공초점 현미경을 통해 이미지로 나타낸 것이다.
도 13은 실험예 4에서 재조합 균주의 비오틴 결합을 공초점 현미경을 통해 이미지로 나타낸 것이다.
도 14는 실험예 5에서 재조합 균주의 비오틴 결합 특이성을 분석한 결과를 그래프로 나타낸 것이다.
도 15는 실험예 5에서 재조합 균주의 비오틴 결합 특이성을 분석한 결과를 그래프로 나타낸 것이다.
도 16은 실험예 5에서 종양 동물 모델에서 재조합 균주의 비오틴 결합을 이미지로 나타낸 것이다.
도 17은 실험예 5에서 종양 동물 모델에서 재조합 균주의 비오틴 결합을 이미지로 나타낸 것이다.
도 18은 실험예 5에서 종양 동물 모델에서 재조합 균주의 비오틴 결합을 이미지로 나타낸 것이다.
도 19는 실험예 5에서 적출된 종양에서 재조합 균주의 비오틴 결합을 이미지로 나타낸 것이다.
도 20은 실험예 5에서 종양 동물 모델에서 재조합 균주의 비오틴 결합을 이미지로 나타낸 것이다.
도 2은 실험예 2에서 MBP-mSA 유전자 발현 결과를 나타낸 것이다.
도 3은 실험예 2에서 MBP-mSA 유전자 발현 및 활성 확인을 위한 웨스턴 블롯 결과를 나타낸 것이다.
도 4는 실험예 2에서 재조합 균주의 비오틴 결합을 분석한 결과를 그래프로 나타낸 것이다.
도 5는 실험예 2에서 재조합 균주의 비오틴 결합을 공초점 현미경을 통해 이미지로 나타낸 것이다.
도 6은 실험예 2에서 재조합 균주의 비오틴 결합을 공초점 현미경을 통해 이미지로 나타낸 것이다.
도 7은 실험예 3에서 MBP-mSA 유전자 발현 결과를 나타낸 것이다.
도 8은 실험예 3에서 MBP-mSA 유전자 발현 및 활성 확인을 위한 웨스턴 블롯 결과를 나타낸 것이다.
도 9는 실험예 4에서 MBP-mSA 유전자 발현 확인을 위한 웨스턴 블롯 결과를 나타낸 것이다.
도 10은 실험예 4에서 MBP-mSA 유전자 발현 비교를 위한 웨스턴 블롯 결과를 나타낸 것이다.
도 11은 실험예 4에서 재조합 균주의 비오틴 결합을 분석한 결과를 그래프로 나타낸 것이다.
도 12는 실험예 4에서 재조합 균주의 비오틴 결합을 공초점 현미경을 통해 이미지로 나타낸 것이다.
도 13은 실험예 4에서 재조합 균주의 비오틴 결합을 공초점 현미경을 통해 이미지로 나타낸 것이다.
도 14는 실험예 5에서 재조합 균주의 비오틴 결합 특이성을 분석한 결과를 그래프로 나타낸 것이다.
도 15는 실험예 5에서 재조합 균주의 비오틴 결합 특이성을 분석한 결과를 그래프로 나타낸 것이다.
도 16은 실험예 5에서 종양 동물 모델에서 재조합 균주의 비오틴 결합을 이미지로 나타낸 것이다.
도 17은 실험예 5에서 종양 동물 모델에서 재조합 균주의 비오틴 결합을 이미지로 나타낸 것이다.
도 18은 실험예 5에서 종양 동물 모델에서 재조합 균주의 비오틴 결합을 이미지로 나타낸 것이다.
도 19는 실험예 5에서 적출된 종양에서 재조합 균주의 비오틴 결합을 이미지로 나타낸 것이다.
도 20은 실험예 5에서 종양 동물 모델에서 재조합 균주의 비오틴 결합을 이미지로 나타낸 것이다.
이하, 실시예를 통하여 본 발명을 더욱 상세히 설명하고자 한다. 이들 실시예는 오로지 본 발명을 보다 구체적으로 설명하기 위한 것으로서, 본 발명의 요지에 따라 본 발명의 범위가 이들 실시예에 의해 제한되지 않는다는 것은 당업계에서 통상의 지식을 가진 자에게 있어서 자명할 것이다.
[실시예 1] mSA 발현 플라스미드 제작
[1-1] mSA 유전자 삽입 플라스미드 제작
재조합 균주에서 mSA 발현을 위한 플라스미드 제작을 위해 서열번호 2로 표시되는 모노머형 스트렙타비딘(mSA) 유전자를 합성(마크로젠, 한국)하여 증폭한 뒤, 제한효소 EcoRⅠ 및 SalⅠ을 넣어 절단하고 이를 정제하여 유전자 증폭 산물을 얻은 뒤에 이를 동일한 제한효소로 절단한 pBAD24 플라스미드에 클로닝 하여 pBAD-mSA(B-mSA) 플라스미드를 제작하였다.
추가로 mSA 유전자의 발현을 증가시키기 위해 프로모터 하류에 하기 표 1의 리보솜 결합 부위(Ribosome Binding Site; RBS)인 BBa_B0032, BBa_B0030, BBa_B0034를 삽입하여 pBAD_RBS 0.3-mSA(B_R0.3-mSA), pBAD_RBS 0.6-mSA(B_R0.6-mSA), pBAD_RBS 1.0-mSA(B_R1.0-mSA) 플라스미드를 제작하였다.
또한, 유전자의 발현과 가용성을 증가시키기 위해 pBAD-mSA 플라스미드를 주형으로 하여 mSA 유전자를 증폭한 뒤, 제한효소 EcoRⅠ 및 HindⅢ을 넣어 절단하고 이를 정제하여 유전자 증폭 산물을 얻은 뒤에 이를 동일한 제한효소로 절단한 pMAl_p2x, pMAl_c2x 플라스미드에 클로닝 하여 pMAl_p2x-mSA(M_p-mSA), pMAl_c2x-mSA(M_c-mSA) 플라스미드를 제작하였다.
[1-2] mSA-MBP 발현 플라스미드 제작
그 다음, 동물실험에 이용할 수 있도록 M-p-mSA 및 M-c-mSA 플라스미드에, 서열번호 4로 표시되는 말토오즈 결합 단백질(MBP)을 코딩하는 유전자와 서열번호 2로 표시되는 mSA 유전자가 융합된 BBa_B0034 서열을 pBAD24에 다시 클로닝하여 pBAD_p2x-mSA(B_p-mSA), pBAD_c2x-mSA(B_c-mSA), pBAD_RBS1-p2x-mSA(B_R1.0-p-mSA), pBAD_RBS1-c2x-mSA(B_R1.0-c-mSA) 플라스미드를 제작하였다.
[1-3] RBS 치환 플라스미드 제작
mSA 발현 수준 및 기능성을 증가시키기 위해서, 기존 RBS를 신규한 조절 유전자로 치환한 유전자 컨스트럭트를 추가로 제작하였다(하기 표 1). 우선 플라스미드의 RBS 서열을 분석하여 서열 라이브러리를 제작하였다. 다음으로 RBS 계산기(Penn State University) 프로그램을 이용하여 B_p-mSA 플라스미드의 번역 개시 속도(TIR)를 분석한 후, RBS 라이브러리 계산기(RBS Library Calculator)를 통하여 번역 개시 속도의 값 범위가 3.97에서 42889인 조절 유전자 라이브러리를 구축하였다. 상기 라이브러리에 따라 제작된 조절 유전자를 B_p-mSA 플라스미드의 RBS 서열과 치환하여 클로닝한 후 생성된 콜로니를 선별하여 최종 플라스미드 pBAD_R01-p2x-mSA(B_R01-p-mSA), pBAD_R02-p2x-mSA(B_R02-p-mSA), pBAD_R1-p2x-mSA(B_R1-p-mSA), pBAD_R11-p2x-mSA(B_R11-p-mSA), pBAD_R12-p2x-mSA(B_R12-p-mSA), pBAD_R13-p2x-mSA(B_R13-p-mSA), pBAD_R2-p2x-mSA(B_R2-p-mSA), pBAD_R21-p2x-mSA(B_R21-p-mSA)를 제작하였다(하기 표 1 참고).
상기 실시예 [1-1] 내지 [1-3]에서 제작한 각 유전자 컨스트럭트의 명칭, 약자, 기본 플라스미드 및 전체 서열은 하기 표 1에 나타낸 바와 같다.
명칭 (약자) |
기본 플라스미드 |
유전자 컨스트럭트 | |||
mSA | MBP | RBS | 전체 서열 | ||
pBAD-mSA (B-mSA) |
pBAD24 | 서열번호 2 | 미추가 | 비치환 | 서열번호 8 |
pBAD_RBS 0.3-mSA (B_R0.3-mSA) |
pBAD24 | 서열번호 2 | 미추가 | 서열번호 26 | 서열번호 9 |
pBAD_RBS 0.6-mSA (B_R0.6-mSA) |
pBAD24 | 서열번호 2 | 미추가 | 서열번호 27 | 서열번호 10 |
pBAD_RBS 1.0-mSA (B_R1.0-mSA) |
pBAD24 | 서열번호 2 | 미추가 | 서열번호 28 | 서열번호 11 |
pMAl_p2x-mSA (M_p-mSA) |
pMAl_p2x | 서열번호 2 | 서열번호 4 | 비치환 | 서열번호 12 |
pMAl_c2x-mSA (M_c-mSA) |
pMAl_c2x | 서열번호 2 | 서열번호 4 | 비치환 | 서열번호 13 |
pBAD_p2x-mSA (B_p-mSA) |
pBAD24 | 서열번호 2 | 서열번호 4 | 서열번호 29 | 서열번호 14 |
pBAD_c2x-mSA (B_c-mSA) |
pBAD24 | 서열번호 2 | 서열번호 4 | 서열번호 29 | 서열번호 15 |
pBAD_RBS1-p2x-mSA (B_R1.0-p-mSA) |
pBAD24 | 서열번호 2 | 서열번호 4 | 서열번호 28 | 서열번호 16 |
pBAD_RBS1-c2x-mSA (B_R1.0-c-mSA) |
pBAD24 | 서열번호 2 | 서열번호 4 | 서열번호 28 | 서열번호 17 |
pBAD_R01-p2x-mSA (B_R01-p-mSA) |
pBAD24 | 서열번호 2 | 서열번호 4 | 서열번호 30 | 서열번호 18 |
pBAD_R02-p2x-mSA (B_R02-p-mSA) |
pBAD24 | 서열번호 2 | 서열번호 4 | 서열번호 31 | 서열번호 19 |
pBAD_R1-p2x-mSA (B_R1-p-mSA) |
pBAD24 | 서열번호 2 | 서열번호 4 | 서열번호 32 | 서열번호 20 |
pBAD_R11-p2x-mSA (B_R11-p-mSA) |
pBAD24 | 서열번호 2 | 서열번호 4 | 서열번호 33 | 서열번호 21 |
pBAD_R12-p2x-mSA (B_R12-p-mSA) |
pBAD24 | 서열번호 2 | 서열번호 4 | 서열번호 34 | 서열번호 22 |
pBAD_R13-p2x-mSA (B_R13-p-mSA) |
pBAD24 | 서열번호 2 | 서열번호 4 | 서열번호 35 | 서열번호 23 |
pBAD_R2-p2x-mSA (B_R2-p-mSA) |
pBAD24 | 서열번호 2 | 서열번호 4 | 서열번호 36 | 서열번호 24 |
pBAD_R21-p2x-mSA (B_R21-p-mSA) |
pBAD24 | 서열번호 2 | 서열번호 4 | 서열번호 37 | 서열번호 25 |
pBAD R-lib-1-1-mSA (R-lib-1-1-mSA) |
pBAD24 | 서열번호 2 | 서열번호 4 | 서열번호 66 | 서열번호 39 |
pBAD R-lib-1-5-mSA (R-lib-1-5-mSA) |
pBAD24 | 서열번호 2 | 서열번호 4 | 서열번호 67 | 서열번호 40 |
pBAD R-lib-1-7-mSA (R-lib-1-7-mSA) |
pBAD24 | 서열번호 2 | 서열번호 4 | 서열번호 68 | 서열번호 41 |
pBAD R-lib-1-10-mSA (R-lib-1-10-mSA) |
pBAD24 | 서열번호 2 | 서열번호 4 | 서열번호 69 | 서열번호 42 |
pBAD R-lib-1-11-mSA (R-lib-1-11-mSA) |
pBAD24 | 서열번호 2 | 서열번호 4 | 서열번호 70 | 서열번호 43 |
pBAD R-lib-1-12-mSA (R-lib-1-12-mSA) |
pBAD24 | 서열번호 2 | 서열번호 4 | 서열번호 71 | 서열번호 44 |
pBAD R-lib-1-13-mSA (R-lib-1-13-mSA) |
pBAD24 | 서열번호 2 | 서열번호 4 | 서열번호 72 | 서열번호 45 |
pBAD R-lib-1-14-mSA (R-lib-1-14-mSA) |
pBAD24 | 서열번호 2 | 서열번호 4 | 서열번호 73 | 서열번호 46 |
pBAD R-lib-1-16-mSA (R-lib-1-16-mSA) |
pBAD24 | 서열번호 2 | 서열번호 4 | 서열번호 74 | 서열번호 47 |
pBAD R-lib-1-17-mSA (R-lib-1-17-mSA) |
pBAD24 | 서열번호 2 | 서열번호 4 | 서열번호 75 | 서열번호 48 |
pBAD R-lib-1-18-mSA (R-lib-1-18-mSA) |
pBAD24 | 서열번호 2 | 서열번호 4 | 서열번호 76 | 서열번호 49 |
pBAD R-lib-2-2-mSA (R-lib-2-2-mSA) |
pBAD24 | 서열번호 2 | 서열번호 4 | 서열번호 77 | 서열번호 50 |
pBAD R-lib-2-3-mSA (R-lib-2-3-mSA) |
pBAD24 | 서열번호 2 | 서열번호 4 | 서열번호 78 | 서열번호 51 |
pBAD R-lib-2-4-mSA (R-lib-2-4-mSA) |
pBAD24 | 서열번호 2 | 서열번호 4 | 서열번호 79 | 서열번호 52 |
pBAD R-lib-2-5-mSA (R-lib-2-5-mSA) |
pBAD24 | 서열번호 2 | 서열번호 4 | 서열번호 80 | 서열번호 53 |
pBAD R-lib-2-6-mSA (R-lib-2-6-mSA) |
pBAD24 | 서열번호 2 | 서열번호 4 | 서열번호 81 | 서열번호 54 |
pBAD R-lib-2-7-mSA (R-lib-2-7-mSA) |
pBAD24 | 서열번호 2 | 서열번호 4 | 서열번호 82 | 서열번호 55 |
pBAD R-lib-2-8-mSA (R-lib-2-8-mSA) |
pBAD24 | 서열번호 2 | 서열번호 4 | 서열번호 83 | 서열번호 56 |
pBAD R-lib-2-14-mSA (R-lib-2-14-mSA |
pBAD24 | 서열번호 2 | 서열번호 4 | 서열번호 84 | 서열번호 57 |
pBAD R-lib-2-16-mSA (R-lib-2-16-mSA |
pBAD24 | 서열번호 2 | 서열번호 4 | 서열번호 85 | 서열번호 58 |
pBAD R-lib-2-17-mSA (R-lib-2-17-mSA |
pBAD24 | 서열번호 2 | 서열번호 4 | 서열번호 86 | 서열번호 59 |
pBAD R-lib-3-4-mSA (R-lib-3-4-mSA |
pBAD24 | 서열번호 2 | 서열번호 4 | 서열번호 87 | 서열번호 60 |
pBAD R-lib-3-5-mSA (R-lib-3-5-mSA |
pBAD24 | 서열번호 2 | 서열번호 4 | 서열번호 88 | 서열번호 61 |
pBAD R-lib-3-11-mSA (R-lib-3-11-mSA |
pBAD24 | 서열번호 2 | 서열번호 4 | 서열번호 89 | 서열번호 62 |
pBAD R-lib-3-13-mSA (R-lib-3-13-mSA |
pBAD24 | 서열번호 2 | 서열번호 4 | 서열번호 90 | 서열번호 63 |
pBAD R-lib-3-18-mSA (R-lib-3-18-mSA |
pBAD24 | 서열번호 2 | 서열번호 4 | 서열번호 91 | 서열번호 64 |
pBAD R-lib-3-20-mSA (R-lib-3-20-mSA |
pBAD24 | 서열번호 2 | 서열번호 4 | 서열번호 92 | 서열번호 65 |
[1-4] 조절 유전자 전사체의 총 깁스 자유 에너지 변화량 계산
상기 실시예 [1-3]에서 제작된 리보솜 결합 부위(RBS)의 프로모터로부터 개시코돈 까지의 서열에서, 총 깁스 자유 에너지 변화량(ΔGtotal)에 따른 상기 유전자 컨스트럭트의 mSA 발현 능력을 확인하기 위해, 조절 유전자의 mRNA와 리보솜의 30S 서브유닛의 복합체를 형성하는 반응이 일어날 때의 깁스 자유 에너지 변화량(ΔGmRNA-rRNA), 상기 조절 유전자의 mRNA 전사체에서 리보솜의 30S 서브유닛의 복합체를 형성하는 서열과 개시코돈 간의 간격(Spacing)이 최적화 되지 않음으로써 발생하는 대한 깁스 자유 에너지 패널티(ΔGspacing), 상기 간격(Spacing)의 영역에서 쌓인 뉴클레오티드의 깁스 자유 에너지 변화량(ΔGstacking), 상기 조절 유전자의 mRNA 전사체의 대기 부위(Stanby Site)와 리보솜 간 결합 반응이 일어나는 경우 깁스 자유 에너지 패널티(ΔGstandby), mRNA-tRNA 복합체를 형성하는 반응이 일어날 때의 깁스 자유 에너지 변화량(ΔGstart) 및 상기 조절 유전자의 mRNA 전사체가 접힌 복합체 구조물을 형성할 때의 깁스 자유 에너지 변화량(ΔGmRNA)을 계산하여 총 깁스 자유 에너지 변화량(ΔGtotal)을 계산한 결과를 하기 표 2에 나타내었다. 여기서, 상기 총 깁스 자유 에너지 변화량(ΔGtotal)은 하기 식 1 및 식 2와 같은 방법으로 계산되었다:
[식 1]
ΔGtotal = (ΔGfinal) - (ΔGinitial)
[식 2]
(ΔGfinal) - (ΔGinitial) = [(ΔGmRNA-rRNA) + (ΔGspacing) + (ΔGstacking) + (ΔGstandby) + (ΔGstart)] - (ΔGmRNA)
RBS | 단위 kcal/mol | ||||||
서열번호 | ΔGmRNA-rRNA | ΔGspacing | ΔGstacking | ΔGstandby | ΔGstart | ΔGmRNA | ΔGtotal |
29 | -14.4914 | 4.992 | 0 | 2.41 | -2.76 | -15.01 | 4.944348676 |
30 | -6.84135 | 1.525 | 0 | 1.5336 | -2.76 | -7.76 | 1.328848924 |
31 | 1.158649 | 0.005326 | 0 | 0.4314 | -0.42 | -3.4 | 4.641874373 |
32 | -7.05135 | 0 | 0 | 1.0898 | -2.76 | -5.79 | -3.555751381 |
33 | -1.37135 | 0 | 0 | 0.0786 | -2.76 | -4.17 | 0.1498488 |
34 | -0.24135 | 0 | 0 | 0.29 | -2.76 | -6.12 | 3.239348447 |
35 | -5.39135 | 0.672 | 0 | 4.0728 | -2.76 | -7.41 | 3.862748471 |
36 | -9.37135 | 0.288 | 0 | 4.0728 | -2.76 | -4.61 | -3.458051381 |
37 | -7.44135 | 0 | 0 | 4.8986 | -2.76 | -8.12 | 2.647948438 |
66 | -7.44135 | 0 | 0 | 4.8986 | -2.76 | -10.25 | 4.777948552 |
67 | -13.8614 | 0.288 | 0 | 4.0728 | -2.76 | -4.62 | -7.9380514 |
68 | -7.77135 | 0.288 | 0 | 4.0728 | -2.76 | -5.98 | -0.488051352 |
69 | -5.39135 | 0.288 | 0 | 4.0728 | -2.76 | -5.98 | 1.891948643 |
70 | -2.68135 | 0.288 | 0 | 3.3234 | -2.76 | -4.61 | 2.482548676 |
71 | -5.39135 | 0.288 | 0 | 4.0728 | -2.76 | -4.61 | 0.521948757 |
72 | -2.68135 | 0.288 | 0 | 4.0728 | -2.76 | -5.37 | 3.991948428 |
73 | -2.56135 | 0 | 0 | 3.3234 | -2.76 | -4.61 | 2.4427488 |
74 | -3.96135 | 0.288 | 0 | 4.8986 | -2.76 | -6.51 | 4.67774886 |
75 | -6.95135 | 0.288 | 0 | 4.8986 | -2.76 | -6.45 | 1.627748371 |
76 | -7.49135 | 0.288 | 0 | 4.0728 | -2.76 | -6.78 | 0.59194881 |
77 | -9.37135 | 0 | 0 | 2.6504 | -2.76 | -5.68 | -4.216651686 |
78 | -7.27135 | 0 | 0 | 2.6504 | -2.76 | -4.59 | -3.283051219 |
79 | -7.27135 | 0 | 0 | 2.6504 | -2.76 | -4.59 | -2.966951219 |
80 | -10.9714 | 0 | 0 | 2.6504 | -2.76 | -3.99 | -7.41975141 |
81 | -8.87135 | 0 | 0 | 2.6504 | -2.76 | -2.9 | -6.510651419 |
82 | -10.9714 | 0 | 0 | 2.6504 | -2.76 | -3.58 | -7.860951495 |
83 | -9.37135 | 0 | 0 | 2.6504 | -2.76 | -4.59 | -5.403751362 |
84 | -8.87135 | 0 | 0 | 2.6504 | -2.76 | -3.82 | -5.729451581 |
85 | -9.37135 | 0 | 0 | 2.6504 | -2.76 | -2.44 | -7.456651457 |
86 | -8.87135 | 0 | 0 | 2.6504 | -2.76 | -2.31 | -7.100651572 |
87 | -7.58135 | 1.525 | 0 | 0.0168 | -2.76 | -4.78 | -4.109751104 |
88 | -5.27135 | 0 | 0 | 0.4314 | -2.76 | -4.46 | -3.219451333 |
89 | -5.27135 | 0 | 0 | 0.4314 | -2.76 | -4.27 | -3.76635139 |
90 | -5.27135 | 0 | 0 | 0.4314 | -2.76 | -5.47 | -2.476551581 |
91 | -5.06135 | 0.005326 | 0 | 0.2168 | -2.76 | -4.1 | -3.800725876 |
92 | -7.75135 | 1.525 | 0 | 0.4314 | -2.76 | -4.95 | -3.695151507 |
[1-5] 조절 유전자의 번역 개시 속도 계산
상기 조절 유전자의 번역 개시 속도(Translation Initiation Rate; TIR)에 따른 플라스미드의 mSA 발현 능력을 확인하기 위해, 상기와 같이 제작된 조절 유전자 서열에 따른 각 번역 개시 속도를 계산하여 하기 표 3와 같이 나타내었다.
조절 유전자 | 번역 개시 속도(au) |
비치환된 경우 | 1 |
서열번호 29 | 133.266441 |
서열번호 30 | 678.2310709 |
서열번호 31 | 152.7003477 |
서열번호 32 | 6110.586323 |
서열번호 33 | 1152.963841 |
서열번호 34 | 287.0559877 |
서열번호 35 | 216.8312084 |
서열번호 36 | 5847.72872 |
서열번호 37 | 374.5906748 |
서열번호 66 | 143.6296318 |
서열번호 67 | 43914.95671 |
서열번호 68 | 1536.365645 |
서열번호 69 | 526.4033937 |
서열번호 70 | 403.5382313 |
서열번호 71 | 975.1871797 |
서열번호 72 | 204.582929 |
서열번호 73 | 410.8314232 |
서열번호 74 | 150.2547754 |
서열번호 75 | 592.8668512 |
서열번호 76 | 944.9445588 |
서열번호 77 | 8227.297678 |
서열번호 78 | 5404.842895 |
서열번호 79 | 4688.141139 |
서열번호 80 | 34778.40472 |
서열번호 81 | 23100.64943 |
서열번호 82 | 42417.31004 |
서열번호 83 | 14037.12899 |
서열번호 84 | 16253.13229 |
서열번호 85 | 35360.78091 |
서열번호 86 | 30125.96282 |
서열번호 87 | 7840.852282 |
서열번호 88 | 5252.334127 |
서열번호 89 | 6718.078741 |
서열번호 90 | 3759.681518 |
서열번호 91 | 6822.815917 |
서열번호 92 | 6506.222717 |
상기 표 3에서 나타낸 바와 같이, 제작된 플라스미드의 조절 유전자 중 서열번호 29 내지 37 및 65 내지 92의 조절 유전자는 번역 개시 속도가 50 내지 9000au 범위에 해당하였고, 그 중 서열번호 32 및 36의 조절 유전자는 번역 개시 속도가 900 내지 9000au 범위에 해당하는 것을 확인하였다.
[1-6] 조절 유전자의 서열 분석
상기 조절 유전자 서열의 AGG, TAGG 또는 ATAGG 서열 포함 여부 및 상기 AGG 서열의 3' 말단에서 개시코돈 까지의 간격(Spacing)에 따른 플라스미드의 mSA 발현 능력을 확인하기 위해, 각 플라스미드에 따른 조절 유전자 서열 및 상기 AGG 서열의 3' 말단에서 개시코돈 까지의 간격(Spacing, 단위 bp)을 하기 표 4와 같이 나타내었다.
조절 유전자 서열 | Spacing(bp) | |
서열번호 26 | TCACACAGGAAAG | 4 |
서열번호 27 | ATTAAAGAGGAGAAA | 5 |
서열번호 28 | AAAGAGGAGAAA | 5 |
서열번호 29 | ACCCGTTTTTTGGGCTAACAGGAGGAAGCTAGCGCTAGC | 14 |
서열번호 30 | TAGCACTCGTTGACATACGGACGTCAC | - |
서열번호 31 | ACTACTGAGGCTACT | 5 |
서열번호 32 | TGGAACAGCTCACGCAAAAATAGGTTTCTT | 6 |
서열번호 33 | CGCTTTTTATCGCAACTCTCTACTGTTTCTCCAT | - |
서열번호 34 | TCTGAGAAAGACACGATCTTACTAG | - |
서열번호 35 | TCTAGAGAAAGAGCGGATCCTACCTAG | - |
서열번호 36 | TCTAGAGAAAGATAGGAGAATACTAG | 10 |
서열번호 37 | TCTAGAGAAAGAGGCGACGGTACTAG | 11 |
서열번호 66 | TCTAGAGAAAGAGGCGAGTGTACTAG | 12 |
서열번호 67 | TCTAGAGAAAGATAGGAGGTTACTAG | 10 |
서열번호 68 | TCTAGAGAAAGAGGGGACACTACTAG | 12 |
서열번호 69 | TCTAGAGAAAGAGCGGAAACTACTAG | - |
서열번호 70 | TTCTAGAGAAAGATTTGAATATACTAG | - |
서열번호 71 | TCTAGAGAAAGAACGGACATTACTAG | - |
서열번호 72 | TCTAGAGAAAGACATGACTATACTAG | - |
서열번호 73 | TCTAGAGAAAGAACTGAAGATACTAG | - |
서열번호 74 | TCTAGAGAAAGAGGCGATCCTACTAG | 12 |
서열번호 75 | TCTAGAGAAAGAAGAGAGCCTACTAG | - |
서열번호 76 | TCTAGAGAAAGACTTGAGGCTACTAG | 7 |
서열번호 77 | GAACCCTAATACATTAGGAGATCTTCT | 9 |
서열번호 78 | GAACCCTAATACATTAGGACATATTCT | 9 |
서열번호 79 | GAACCCTAATACATTAGGACATCATCT | 9 |
서열번호 80 | GAACCCTAATACATAAGGAGATCATAT | 9 |
서열번호 81 | GAACCCTAATACATAAGGACATAATAT | 9 |
서열번호 82 | GAACCCTAATACATAAGGAGATTATCT | 9 |
서열번호 83 | GAACCCTAATACATTAGGAGATTATAT | 9 |
서열번호 84 | GAACCCTAATACATAAGGACATCTTAT | 9 |
서열번호 85 | GAACACTAATACATTAGGAGATCTTCT | 9 |
서열번호 86 | GAACACTAATACATAAGGACATAATAT | 9 |
서열번호 87 | TTAAGTAGTTAAACAGGGTATATAGGGGAAGA | 6 |
서열번호 88 | TTAAGTAGTTAAACAGGGTATATAGGACGAGA | 6 |
서열번호 89 | TTAAGTAGTTAAACAGGGTATATAGGGCTATA | 6 |
서열번호 90 | TTAAGTAGTTAAACAGGGTATATAGGAGGATA | 6 |
서열번호 91 | TTAAGTAGTTAAACAGGGTATATAGGGCGATA | 6 |
서열번호 92 | TTAAGTAATTAAACAGGGTATATAGGGGAAGA | 6 |
상기 표 4에서 나타낸 바와 같이, 제작된 플라스미드의 조절 유전자 중 서열번호 26, 27, 28, 29, 31, 32, 36, 37, 66, 67, 68, 74, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91 및 92의 조절 유전자는 AGG 서열을 포함하고 있었고, 그 중 서열번호 32 및 36의 조절 유전자는 상기 AGG 서열의 3' 말단에서 개시코돈 까지의 간격(Spacing)이 6 내지 13bp인 것을 확인하였다. 또한 제작된 플라스미드의 조절 유전자 중 서열번호 32,36, 67, 77, 78, 79, 83, 85, 87, 88, 89, 90, 91 및 92의 조절 유전자는 TAGG 또는 ATAGG 서열을 포함하는 것을 확인하였다.
[실시예 2] 숙주 세포의 형질 전환 및 배양
상기 실시예 1에서 제작한 플라스미드를 대장균(E.coli: DH5a 또는 MG1655), 살모넬라(Salmonella: SHJ2037) 균주에 형질 전환 한 뒤, 엠피실린(Ampicillin)이 포함된 LB 고체 배지를 이용하여 상기 형질 전환된 각각의 균주를 밤새 배양하였다. 그 뒤, 생성된 콜로니를 새로 항생제가 포함된 LB 액체 배지를 사용하여 1:100의 비율로 희석하고, 추가 배양을 통해 OD600 값이 0.5 내지 0.7에 도달할 때, 상기 배양액에 최종 농도가 0.1%인 아라비노오스(arabinose)를 첨가하고 200rpm 및 37℃의 조건으로 진탕 배양기에서 배양하였다.
[실험예 1] 재조합 mSA 플라스미드의 mSA 발현 수준 확인
mSA 유전자를 단독으로 삽입한 플라스미드의 발현 수준을 확인하기 위해 상기 실시예 1에서 제작된 플라스미드 B-mSA, B_R0.3-mSA, B_R0.6-mSA, B_R1.0-mSA를 포함하는 재조합 대장균 콜로니를 실시예 2와 같이 배양한 후, 배양된 재조합 대장균을 OD4 기준으로 SDS-PAGE 샘플 버퍼를 첨가하여 95℃에서 10분간 끓인 후 SDS-PAGE에 로딩하여 단백질 발현 정도를 확인하여 도 1에 나타내었다.
도 1에서 나타난 바와 같이, 단백질 발현을 개선하기 위해 BBa_B0032, BBa_B0030, BBa_B0034로 알려진 RBS 서열을 mSA 유전자 서열 앞에 삽입한 플라스미드를 포함한 균주들은 SDS-PAGE 상에서 mSA 단백질 발현을 확인할 수 없었다. 이를 통해서 mSA 유전자를 단독 발현함에 있어서 pBAD 발현 시스템에서는 RBS 서열의 추가는 유전자가 과발현에 영향을 크게 미치지 않음을 알 수 있었다.
[실험예 2] MBP-mSA 플라스미드의 mSA 발현 수준 및 활성 확인
[2-1] SDS-PAGE
mSA의 발현과 가용성을 증대시키기 위해 MBP 유전자를 융합한 MBP-mSA 플라스미드로 형질 전환된 균주의 mSA 발현 수준을 확인하였다. 구체적으로는, MBP 유전자를 융합한 M_p-mSA, M_c-mSA 플라스미드를 상기 실시예 1과 같이 제작한 후, 상기 실시예 2와 같이 형질 전환 및 배양하였다. 배양 중 OD600 값이 0.5 내지 0.7에 도달할 때 상기 배양액에 최종 농도가 0.1 mM인 IPTG(Isopropyl beta-D-1-thiogalactopyranoside)를 첨가하고 200 rpm 및 37℃의 조건으로 진탕 배양기에서 배양하였다. 배양된 재조합 대장균은 OD4 기준으로 SDS-PAGE sample buffer를 첨가하여 95도에서 10분간 끓인 후 SDS-PAGE 로딩 및 단백질 발현 수준을 확인하여 도 2에 나타내었다.
그 결과, 도 2와 같이, MBP와 융합한 mSA 단백질은 겔(gel) 상에서 과발현됨을 확인하였으며 분비 서열이 없는 MBP 유전자를 융합한 mSA 유전자가 분비 서열이 있는 경우 보다 더 과발현됨을 알 수 있었다.
[2-2] 웨스턴 블롯 분석
MBP-mSA 플라스미드로 형질 전환된 재조합 균주의 mSA 발현 수준과 비오틴 결합 활성을 확인하기 위해 웨스턴 블롯 분석을 수행하였다. 구체적으로, 상기 실시예 2의 균주의 배양액을 4 x 107 CFU/ml이 되도록 PBS를 이용하여 희석하고, 13,000rpm에서 5분동안 원심분리 하여 펠렛을 수집하였다. 상기 펠렛 분획을 PBS를 이용하여 세척하고, 0.2% 베타-머캅토에탄올(beta-mercaptoethanol)(카탈로그 번호: EBA-1052, ELPIS BIOTECH)이 포함된 SDS 샘플 완충액과 혼합하여 균주 용해물을 수득하였다. 그런 다음, 12% SDS-PAGE에서 상기 균주 용해물을 전기영동 하고, 상기 겔로부터 니트로 셀룰로스 막에 단백질을 옮긴 뒤 5% 탈지유를 이용하여 상온에서 블로킹 하였다. 이후, his tag 항체를 이용하여 mSA의 발현 수준을 확인하고, 비오틴화된 과산화효소(Biotinylated peroxidase)를 이용하여 mSA의 비오틴 결합 활성을 확인하였다. 수행하여 도 3에 나타내었다.
도 3에서 나타난 바와 같이, MBP 유전자와 mSA 유전자를 모두 삽입한 M_c-mSA 및 M_p-mSA 플라스미드의 MBP와 융합한 mSA 단백질 발현량이 단순 mSA 유전자만 삽입한 B-mSA 플라스미드의 MBP와 융합하지 않은 단순 mSA 단백질 발현량 보다 높았다.
또한, M_c-mSA 플라스미드에서 발현된 단백질이 M_p-mSA 보다 많음에도 불구하고 biotin과의 결합 활성은 분비 서열이 있는 MBP와 융합한 mSA가 더 높음을 확인할 수 있었다.
[2-3] 비오틴 흡수 분석
MBP-mSA 플라스미드로 형질 전환된 재조합 균주의 비오틴 결합 활성을 분석하기 위해 비오틴 흡수 분석(biotin uptake assay)을 수행하여 그 결과를 도 4와 같이 나타내었다. 구체적으로, 배양된 균주에 비오틴화된 형광염료(biotinylated fluorescent dye, biotin-flamma 675 dye, BioActs)를 첨가하여 반응한 후, PBS로 세척하여 균주에 결합하지 않는 비오틴화된 형광염료를 제거하여 형광 측정 리더기(Infinite m200, Tecan)를 이용하여 재조합 균주에 흡수된 형광염료의 형광값을 측정하였다.
그 결과, 도 4와 같이, 아라비노즈 첨가 전/후 비오틴 결합 시그널 증가량이 대조군인 B-mSA 플라스미드를 포함하는 균주(344%)가 pBAD(151%), M_c-mSA(141%) 및 M_p-mSA(158%)를 포함하는 균주보다 비오틴 활성이 더 높은 것을 알 수 있었다.
[2-4] 공초점 현미경 관찰
실제 재조합 균주와 비오틴화된 형광염료의 결합을 이미지로 확인하기 위해 상기 실시예 2와 같이 배양한 재조합 균주를 슬라이드에 고정하여 공초점 현미경으로 확인하여 도 5 및 도 6과 같이 나타내었다.
도 5 및 도 6과 같이 비오틴 흡수 분석(biotin uptake assay)의 결과와 달리 MBP 융합 여부와 상관없이 균주에 결합한 비오틴화된 형광염료는 매우 적은 수를 보였다. 이를 통해 MBP 유전자 융합을 통해 mSA의 발현이 개선되었으나, 유전자의 발현 및 가용성의 변화가 비오틴 결합 활성을 개선시킬 수 없다는 것을 확인할 수 있었다.
[실험예 3] RBS 추가 플라스미드의 mSA 발현 수준 및 활성 확인
[3-1] SDS-PAGE
RBS가 추가된 플라스미드로 형질 전환된 재조합 균주의 mSA의 발현 및 활성을 확인하기 위해 SDS-PAGE를 수행하였다. 구체적으로는, MBP-mSA 유전자를 pBAD 플라스미드에 옮긴 B_p-mSA, B_c-mSA 및 상기 플라스미드의 발현을 개선하기 위해 BBa_B0034 서열을 추가한 B_R1.0-p-mSA, B_R1.0-c-mSA 플라스미드로 형질 전환된 재조합 균주에 대하여 SDS-PAGE를 수행한 결과를 도 7과 같이 나타내었다.
도 7에서 나타난 바와 같이, 상기 재조합 균주를 SDS-PAGE에 로딩하여 단백질 발현 정도를 확인한 결과, pBAD 플라스미드에서도 MBP와 융합한 mSA 단백질은 과발현됨을 확인하였다.
[3-2] 웨스턴 블롯 분석
RBS가 추가된 플라스미드로 형질 전환된 재조합 균주의 mSA의 발현 및 활성을 확인하기 위해 웨스턴 블롯 분석을 수행하였다. MBP-mSA 유전자를 pBAD 플라스미드에 옮긴 B_p-mSA, B_c-mSA와 이 플라스미드의 발현을 개선하기 위해 BBa_B0034 서열을 추가한 B_R1.0-p-mSA, B_R1.0-c-mSA 플라스미드에 대하여 상기 실험예 2-2와 같은 방식으로 웨스턴 블롯 분석을 수행하여 도 8과 같이 나타내었다.
도 8에서 나타난 바와 같이, 웨스턴 블롯 분석을 수행한 결과, MBP 유전자와 mSA 유전자를 모두 삽입한 B_p-mSA, B_c-mSA, B_R1.0-p-mSA 및 B_R1.0-c-mSA 플라스미드는 MBP와 융합한 mSA 단백질을 과발현하는 것을 확인하였으며, 이는 MBP와 융합하지 않은 단순 mSA 단백질 발현량 보다 높았다.
[실험예 4] RBS 치환 플라스미드의 mSA 발현 수준 및 활성 확인
[4-1] 웨스턴 블롯 분석(1)
따라서, 본 발명자는 재조합 균주에서 유전자의 기능성 발현이 증가하게 유도되도록 하기 위해서 B_p-mSA 플라스미드의 RBS 서열을 분석하여 상기 실시예 1과 같이 B_R01-p-mSA, B_R02-p-mSA, B_R1-p-mSA, B_R11-p-mSA, B_R12-p-mSA, B_R13-p-mSA B_R2-p-mSA, B_R21-p-mSA 플라스미드를 제작한 후 균주에 형질 전환 및 배양하였다. 상기 재조합 균주의 단백질 발현 정도를 확인하기 위해 상기 실험예 2-2와 같은 방식으로 웨스턴 블롯 분석을 수행하여 도 9와 같이 나타내었다.
도 9에서 나타난 바와 같이, mSA를 발현하는 균주 중 B_R1-p-mSA와 B_R2-p-mSA 플라스미드로 형질 전환된 재조합 균주가 다른 균주에 비해 mSA 발현 수준이 뛰어난 것을 확인하였다.
[4-2] 웨스턴 블롯 분석(2)
mSA 발현 수준이 높은 B_R1-p-mSA와 B_R2-p-mSA 플라스미드로 형질 전환된 상기 두 균주를 선정하여 추가 실험을 수행하여, 그 결과를 도 10에 나타내었다.
도 10에서 나타난 바와 같이, 대조군에서는 M_p-mSA 플라스미드를 포함하는 재조합 균주가 B_p-mSA 플라스미드를 포함하는 재조합 균주보다 mSA의 발현과 분비 수준이 높았다. 실험군에서도 M_p-mSA 플라스미드를 포함하는 재조합 균주가 B_R1-p-mSA와 B_R2-p-mSA 플라스미드를 포함하는 재조합 균주 보다 mSA의 발현과 분비 수준이 높음을 확인하였다.
또한 B_R1-p-mSA와 B_R2-p-mSA 플라스미드를 포함하는 재조합 균주가 M_p-mSA 플라스미드를 포함하는 재조합 균주보다 발현양이 더 높지만 분비된 단백질은 더 적은 것으로 나타나서, 상기 B_R1-p-mSA와 B_R2-p-mSA 플라스미드에서 발현된 mSA는 균주의 주변세포질(periplasm)에 머무는 것을 알 수 있었다. 비오틴 결합 활성은 B_p-mSA, B_R1-p-mSA, M_p-mSA, B_R2-p-mSA 플라스미드를 포함하는 재조합 균주 순이였으며, 분비된 단백질 결합 활성은 M_p-mSA, B_p-mSA, B_R1-p-mSA, B_R2-p-mSA 플라스미드를 포함하는 재조합 균주 순으로 확인되었다.
[4-3] 비오틴 흡수 분석
또한, 발현이 개선된 재조합 균주의 비오틴 결합 활성을 분석하기 위해 실험예 2와 같은 방식으로 비오틴 흡수 분석(biotin uptake assay)을 수행하여 그 결과를 도 11과 같이 나타내었다.
도 11에서 나타난 바와 같이, 대조군으로 pBAD와 B-mSA 플라스미드를 포함하는 재조합 균주보다, B_R1-p-mSA와 B_R2-p-mSA 플라스미드를 포함하는 재조합 균주가 비오틴 결합 활성이 현저히 높아, 상기 B_R1-p-mSA와 B_R2-p-mSA 플라스미드의 유전자에서 발현된 mSA가 다른 플라스미드에서 발현된 mSA에 비하여 현저한 비오틴 결합 활성 효과를 가지는 것을 확인하였다. 또한, 비오틴 결합 활성은 단백질 발현량과는 비례하지 않아, 단순히 단백질 발현량 만으로는 비오틴 결합 활성 효과를 예측할 수 없음을 확인하였다.
[4-4] 공초점 현미경 관찰
실제 재조합 균주와 비오틴화된 형광염료의 결합을 이미지로 확인하기 위해 상기 배양한 균주를 슬라이드에 고정하여 도 12 및 도 13과 같이 공초점 현미경으로 확인하였다.
도 12에서 나타난 바와 같이, B_R1-p-mSA와 B_R2-p-mSA 플라스미드를 포함하는 재조합 균주는 도 13에 나타난 대조군인 B-mSA 및 B_p-mSA에 비하여 비오틴화된 형광염료가 균주에 강하게 결합하는 것을 확인할 수 있었으며, 특히, B_R2-p-mSA 플라스미드에 의한 mSA 발현이 비오틴화된 형광염료와 결합하기에 최적의 상태임을 알 수 있었다. 이를 통해 MBP 유전자 융합을 통해 mSA 유전자의 발현이 개선된 균주의 경우에도 외부 비오틴과의 충분한 결합을 하지 못하였으나, mSA 유전자와 함께 MBP 유전자 및 RBS 유전자를 결합시킨 경우 mSA 유전자의 발현이 보다 기능적으로 개선되어 외부 비오틴과의 결합능이 매우 향상됨을 알 수 있었다.
[실험예 5] mSA 발현 재조합 균주의 비오틴 결합 특이성 확인
[5-1] 비오틴 흡수 분석
상기 제작된 본 발명의 재조합 균주에서 발현된 mSA 유전자가 비오틴(biotin)에 특이성을 갖는지 확인하기 위해 살모넬라(Salmonella) 균주에 pBAD, B-mSA, B_p-mSA, B_R1-p-mSA, B_R2-p-mSA 플라스미드를 실험예 1과 같이 형질 전환 및 배양 후, 상기 실험예 2와 같은 방식으로 비오틴 흡수 분석(biotin uptake assay)을 수행하여 그 결과를 도 14 및 도 15에 나타내었다.
도 14에서 나타난 바와 같이, 비오틴화된 형광염료만 처리하였을 때 B_R1-p-mSA 플라스미드를 포함하는 재조합 살모넬라(Salmonella)균주가 가장 높은 비오틴 흡수(biotin uptake)를 보였다. 반면, 도 15에서 나타난 바와 같이, 형광염료가 없는 비오틴을 미리 첨가한 후 비오틴화된 형광염료를 처리하였을 때 재조합 균주의 비오틴화된 형광염료의 흡수가 감소함을 확인하였다. 상기 도 14와 도 15 간의 차이를 통하여 비오틴화된 형광염료를 첨가하기 전에 형광염료가 없는 200 nM의 비오틴을 첨가하는 경우 비오틴화된 형광염료와 재조합 균주의 결합 저해할 수 있다는 것을 확인하였고, 이를 통하여 본 발명의 재조합 균주는 비오틴에 특이적으로 결합함을 알 수 있었다.
[5-2] 종양 영상화 분석(1)
*본 발명의 재조합 균주에서 비오틴 결합 활성을 확인하기 위해 in vivo 이미지 시스템(In Vivo Imaging System; IVIS) 촬영을 실시하였다. 구체적으로는, 먼저 CT26 세포주를 Balb/c 쥐의 옆구리에 피하 주사하여, 종양 동물 모델을 구축하였다. 상기 종양 동물 모델에 재조합 균주를 각각 주입하였고, 대조군으로는 상기 종양 동물 모델에 염색약만 주입(only dye)하였다. 상기 재조합 균주는 각각 B-mSA, B_p-mSA, B_R2-p-mSA(non-induction) 및 B_R2-p-mSA로 형질 전환된 것이다. 이후 3일 뒤, 비오틴화된 형광염료를 주입하여, 6시간 후 IVIS 촬영을 실시하여 도 16과 같이 나타내었고, 9시간 후 IVIS 촬영을 실시하여 도 17과 같이 나타내었으며, 24시간 후 IVIS 촬영을 실시하여 도 18과 같이 나타내었다.
도 16 내지 도 18에서 나타난 바와 같이, 대조군으로서 염색약만 주입한 종양 동물 모델(only dye), 그리고 B-mSA, B_p-mSA, B_R2-p-mSA(non-induction) 플라스미드로 형질 전환된 재조합 균주가 주입된 종양 동물 모델에 각각 주입된 비오틴화된 형광염료는, 주입된 후 시간이 지남에 따라 점차 생체 내에서 소거되어, 종양에 특이성이 없음을 알 수 있었다. 반면, B_R2-p-mSA 플라스미드를 포함한 재조합 균주를 주입한 종양 동물 모델은 암조직에서 비오틴화된 형광염료를 주입 후 대조군에 비하여 종양에서 강한 시그널을 보였고, 비오틴화된 형광염료를 주입 후 24시간이 지난 후에도 시그널이 여전히 강하게 유지됨을 확인하였다. 이를 통하여, 소동물에서는 본 발명의 재조합 균주에만 비오틴화된 형광염료가 강하게 결합됨을 확인하였으며 특히, 종양 특이성이 있는 재조합 균주를 이용하면, 상기 비오틴화된 형광염료가 재조합 균주를 통하여 종양에 특이적으로 결합할 수 있어, 상기 비오틴화된 형광염료에서 발생한 신호를 촬영 수단으로 확인하여, 실시간 종양 영상화가 가능함을 알 수 있었다.
[5-3] 종양 영상화 분석(2)
또한 본 발명의 재조합 균주에서 비오틴 결합 활성을 확인하기 위해 상기 종양 동물 모델에서 암조직을 적출하여 in vivo 이미지 시스템(In Vivo Imaging System; IVIS) 촬영을 실시하였다. 구체적으로는 상기 종양 동물 모델에 비오틴화된 형광염료를 주입한지 24시간 이후, 각 그룹의 종양을 적출하여 비오틴화된 형광염료의 시그널을 확인하기 위하여 IVIS 촬영을 실시하여 도 19에 나타내었다.
도 19에서 나타난 바와 같이, 다른 그룹에 비해 B_R2-p-mSA 플라스미드를 포함한 재조합 균주 처리군이 강한 형광 활성을 유지하고 있었다. 이를 통하여 소동물에서 본 발명의 재조합 균주를 이용했을 경우에만 비오틴화된 형광염료가 강하게 결합됨을 확인하였으며, 특히 종양 특이성이 있는 재조합 균주를 이용한 실시간 종양 영상화가 가능함을 알 수 있었다.
[5-4] 종양 영상화 분석(3)
본 발명의 재조합 균주에서 비오틴 다회 결합을 확인하기 위해 in vivo 이미지 시스템(In Vivo Imaging System; IVIS) 촬영을 실시하였다. 구체적으로는, 먼저 CT26 세포주를 Balb/c 쥐의 옆구리에 피하 주사하여, 종양 동물 모델을 구축하였다. 상기 종양 동물 모델에 재조합 균주를 주입한 후 3일 뒤, 1차로 비오틴화된 형광염료를 주입하였다. 이후 2일 뒤 같은 종양 동물 모델에 2차로 비오틴화된 형광염료를 주입하였다. 1차 형광염료 주입 전, 주입 6시간 후 및 주입 9시간 후에 IVIS 촬영이 이루어졌고, 이후 2차 형광염료 주입 전, 주입 6시간 후 및 주입 9시간 후에도 IVIS 촬영을 실시하여 도 20에 나타내었다.
도 20에서 나타난 바와 같이, 1차 주입한 비오틴화된 형광염료는 시간이 지남에 따라 암조직에서 본 발명의 재조합 균주에 의해 비오틴화된 형광염료의 시그널이 강하게 유지됨을 확인하였으며 비오틴화된 형광염료가 생채 내에서 소거된 후 다시 2차 주입한 비오틴화된 형광염료에 의해 시간이 지남에 따라 암조직에서 비오틴화된 형광염료의 시그널이 강하게 유지됨을 확인하였다. 이를 통하여 본 발명의 재조합 균주의 mSA 발현을 조절함으로서 비오틴화된 형광염료를 다회 처리하여도 지속적으로 종양 이미지를 확인할 수 있다는 것 및 비오틴화 컨쥬게이트를 시간을 조절하여 구분하여 처리할 수 있음 의미한다.
구체적으로 상기 실험을 통해, 본 발명에 따르는 유전자 컨스트럭트, 특히는 본 발명에 따르는 조절 유전자를 포함하는 경우 발현되는 모노머형 스트렙타비딘(mSA)의 안정성이 뛰어나며 외부 비오틴과 강하게 결합할 수 있고, 이는 생체 내에서도 유효하며, 비오틴화된 형광염료를 다회 처리하거나 시간을 조절하여 처리할 수도 있음을 확인하였다.
이상으로 본 발명의 특정한 부분을 상세히 기술하였는 바, 당업계의 통상의 지식을 가진 자에게 있어서 이러한 구체적인 기술은 단지 바람직한 구현 예일 뿐이며, 이에 본 발명의 범위가 제한되는 것이 아닌 점은 명백하다. 따라서, 본 발명의 실질적인 범위는 첨부된 청구항과 그의 등가물에 의하여 정의된다고 할 것이다.
<110> Industry Foundation of Channam National University
<120> Composition for targeting cancer cell comprising a strain expressing monomeric streptavidin and a biotinylated compound
<130> PDPB204170k01
<150> KR 10-2020-0143546
<151> 2020-10-30
<160> 92
<170> KoPatentIn 3.0
<210> 1
<211> 115
<212> PRT
<213> Artificial Sequence
<220>
<223> mSA protein
<400> 1
Met Ala Glu Ala Gly Ile Thr Gly Thr Trp Tyr Asn Gln His Gly Ser
1 5 10 15
Thr Phe Thr Val Thr Ala Gly Ala Asp Gly Asn Leu Thr Gly Gln Tyr
20 25 30
Glu Asn Arg Ala Gln Gly Thr Gly Cys Gln Asn Ser Pro Tyr Thr Leu
35 40 45
Thr Gly Arg Tyr Asn Gly Thr Lys Leu Glu Trp Arg Val Glu Trp Asn
50 55 60
Asn Ser Thr Glu Asn Cys His Ser Arg Thr Glu Trp Arg Gly Gln Tyr
65 70 75 80
Gln Gly Gly Ala Glu Ala Arg Ile Asn Thr Gln Trp Asn Leu Thr Tyr
85 90 95
Glu Gly Gly Ser Gly Pro Ala Thr Glu Gln Gly Gln Asp Thr Phe Thr
100 105 110
Lys Val Lys
115
<210> 2
<211> 348
<212> DNA
<213> Artificial Sequence
<220>
<223> mSA gene
<400> 2
atggcggaag cgggtatcac cggcacgtgg tacaaccagc atggttctac cttcaccgtt 60
accgcgggtg cggacggtaa cctgaccggt cagtacgaaa accgtgcgca gggcactggt 120
tgccagaact ctccgtacac cctgaccggt cgttacaacg gtaccaaact ggaatggcgt 180
gttgaatgga acaactctac cgaaaactgc cactctcgta ccgaatggcg tggtcagtac 240
cagggtggtg cggaagcgcg tatcaacacc cagtggaacc tgacctacga aggtggttct 300
ggtccggcga ccgaacaggg tcaggacacc ttcaccaaag ttaaataa 348
<210> 3
<211> 392
<212> PRT
<213> Artificial Sequence
<220>
<223> MBP protein
<400> 3
Met Lys Ile Lys Thr Gly Ala Arg Ile Leu Ala Leu Ser Ala Leu Thr
1 5 10 15
Thr Met Met Phe Ser Ala Ser Ala Leu Ala Lys Ile Glu Glu Gly Lys
20 25 30
Leu Val Ile Trp Ile Asn Gly Asp Lys Gly Tyr Asn Gly Leu Ala Glu
35 40 45
Val Gly Lys Lys Phe Glu Lys Asp Thr Gly Ile Lys Val Thr Val Glu
50 55 60
His Pro Asp Lys Leu Glu Glu Lys Phe Pro Gln Val Ala Ala Thr Gly
65 70 75 80
Asp Gly Pro Asp Ile Ile Phe Trp Ala His Asp Arg Phe Gly Gly Tyr
85 90 95
Ala Gln Ser Gly Leu Leu Ala Glu Ile Thr Pro Asp Lys Ala Phe Gln
100 105 110
Asp Lys Leu Tyr Pro Phe Thr Trp Asp Ala Val Arg Tyr Asn Gly Lys
115 120 125
Leu Ile Ala Tyr Pro Ile Ala Val Glu Ala Leu Ser Leu Ile Tyr Asn
130 135 140
Lys Asp Leu Leu Pro Asn Pro Pro Lys Thr Trp Glu Glu Ile Pro Ala
145 150 155 160
Leu Asp Lys Glu Leu Lys Ala Lys Gly Lys Ser Ala Leu Met Phe Asn
165 170 175
Leu Gln Glu Pro Tyr Phe Thr Trp Pro Leu Ile Ala Ala Asp Gly Gly
180 185 190
Tyr Ala Phe Lys Tyr Glu Asn Gly Lys Tyr Asp Ile Lys Asp Val Gly
195 200 205
Val Asp Asn Ala Gly Ala Lys Ala Gly Leu Thr Phe Leu Val Asp Leu
210 215 220
Ile Lys Asn Lys His Met Asn Ala Asp Thr Asp Tyr Ser Ile Ala Glu
225 230 235 240
Ala Ala Phe Asn Lys Gly Glu Thr Ala Met Thr Ile Asn Gly Pro Trp
245 250 255
Ala Trp Ser Asn Ile Asp Thr Ser Lys Val Asn Tyr Gly Val Thr Val
260 265 270
Leu Pro Thr Phe Lys Gly Gln Pro Ser Lys Pro Phe Val Gly Val Leu
275 280 285
Ser Ala Gly Ile Asn Ala Ala Ser Pro Asn Lys Glu Leu Ala Lys Glu
290 295 300
Phe Leu Glu Asn Tyr Leu Leu Thr Asp Glu Gly Leu Glu Ala Val Asn
305 310 315 320
Lys Asp Lys Pro Leu Gly Ala Val Ala Leu Lys Ser Tyr Glu Glu Glu
325 330 335
Leu Ala Lys Asp Pro Arg Ile Ala Ala Thr Met Glu Asn Ala Gln Lys
340 345 350
Gly Glu Ile Met Pro Asn Ile Pro Gln Met Ser Ala Phe Trp Tyr Ala
355 360 365
Val Arg Thr Ala Val Ile Asn Ala Ala Ser Gly Arg Gln Thr Val Asp
370 375 380
Glu Ala Leu Lys Asp Ala Gln Thr
385 390
<210> 4
<211> 1179
<212> DNA
<213> Artificial Sequence
<220>
<223> MBP gene
<400> 4
atgaaaataa aaacaggtgc acgcatcctc gcattatccg cattaacgac gatgatgttt 60
tccgcctcgg ctctcgccaa aatcgaagaa ggtaaactgg taatctggat taacggcgat 120
aaaggctata acggtctcgc tgaagtcggt aagaaattcg agaaagatac cggaattaaa 180
gtcaccgttg agcatccgga taaactggaa gagaaattcc cacaggttgc ggcaactggc 240
gatggccctg acattatctt ctgggcacac gaccgctttg gtggctacgc tcaatctggc 300
ctgttggctg aaatcacccc ggacaaagcg ttccaggaca agctgtatcc gtttacctgg 360
gatgccgtac gttacaacgg caagctgatt gcttacccga tcgctgttga agcgttatcg 420
ctgatttata acaaagatct gctgccgaac ccgccaaaaa cctgggaaga gatcccggcg 480
ctggataaag aactgaaagc gaaaggtaag agcgcgctga tgttcaacct gcaagaaccg 540
tacttcacct ggccgctgat tgctgctgac gggggttatg cgttcaagta tgaaaacggc 600
aagtacgaca ttaaagacgt gggcgtggat aacgctggcg cgaaagcggg tctgaccttc 660
ctggttgacc tgattaaaaa caaacacatg aatgcagaca ccgattactc catcgcagaa 720
gctgccttta ataaaggcga aacagcgatg accatcaacg gcccgtgggc atggtccaac 780
atcgacacca gcaaagtgaa ttatggtgta acggtactgc cgaccttcaa gggtcaacca 840
tccaaaccgt tcgttggcgt gctgagcgca ggtattaacg ccgccagtcc gaacaaagag 900
ctggcaaaag agttcctcga aaactatctg ctgactgatg aaggtctgga agcggttaat 960
aaagacaaac cgctgggtgc cgtagcgctg aagtcttacg aggaagagtt ggcgaaagat 1020
ccacgtattg ccgccactat ggaaaacgcc cagaaaggtg aaatcatgcc gaacatcccg 1080
cagatgtccg ctttctggta tgccgtgcgt actgcggtga tcaacgccgc cagcggtcgt 1140
cagactgtcg atgaagccct gaaagacgcg cagacttaa 1179
<210> 5
<211> 3
<212> DNA
<213> Artificial Sequence
<220>
<223> DNA site
<400> 5
agg 3
<210> 6
<211> 4
<212> DNA
<213> Artificial Sequence
<220>
<223> DNA site
<400> 6
tagg 4
<210> 7
<211> 5
<212> DNA
<213> Artificial Sequence
<220>
<223> DNA site
<400> 7
atagg 5
<210> 8
<211> 4871
<212> DNA
<213> Artificial Sequence
<220>
<223> B-mSA
<400> 8
atcgatgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac 60
tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca 120
ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta 180
aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata 240
ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag 300
cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag 360
caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg 420
tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct 480
tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc 540
ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc 600
gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca 660
tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga 720
tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa 780
acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata 840
taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc 900
ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt 960
tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat 1020
tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta 1080
accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt 1140
aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca 1200
ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta 1260
tcgcaactct ctactgtttc tccatacccg tttttttggg ctagcatggc ggaagcgggt 1320
atcaccggca cgtggtacaa ccagcatggt tctaccttca ccgttaccgc gggtgcggac 1380
ggtaacctga ccggtcagta cgaaaaccgt gcgcagggca ctggttgcca gaactctccg 1440
tacaccctga ccggtcgtta caacggtacc aaactggaat ggcgtgttga atggaacaac 1500
tctaccgaaa actgccactc tcgtaccgaa tggcgtggtc agtaccaggg tggtgcggaa 1560
gcgcgtatca acacccagtg gaacctgacc tacgaaggtg gttctggtcc ggcgaccgaa 1620
cagggtcagg acaccttcac caaagttaaa caccaccacc accaccacta agtcgacctg 1680
caggcatgca agcttggctg ttttggcgga tgagagaaga ttttcagcct gatacagatt 1740
aaatcagaac gcagaagcgg tctgataaaa cagaatttgc ctggcggcag tagcgcggtg 1800
gtcccacctg accccatgcc gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg 1860
gggtctcccc atgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc 1920
gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac 1980
aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg 2040
acgcccgcca taaactgcca ggcatcaaat taagcagaag gccatcctga cggatggcct 2100
ttttgcgttt ctacaaactc ttttgtttat ttttctaaat acattcaaat atgtatccgc 2160
tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta 2220
ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg 2280
ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg 2340
gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac 2400
gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg 2460
acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt 2520
actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg 2580
ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac 2640
cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt 2700
gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag 2760
caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc 2820
aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc 2880
ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta 2940
tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg 3000
ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga 3060
ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttacgcg 3120
ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca 3180
cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc 3240
gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct 3300
ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg 3360
ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc 3420
ttgttccaaa cttgaacaac actcaaccct atctcgggct attcttttga tttataaggg 3480
attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg 3540
aattttaaca aaatattaac gtttacaatt taaaaggatc taggtgaaga tcctttttga 3600
taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt 3660
agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 3720
aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 3780
ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta 3840
gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 3900
aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 3960
aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca 4020
gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga 4080
aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 4140
aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 4200
cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 4260
cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 4320
tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt 4380
tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga 4440
ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca 4500
ccgcataggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg 4560
gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg 4620
tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agcaaggaga tggcgcccaa 4680
cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc 4740
gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc 4800
acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatct gctcatgttt 4860
gacagcttat c 4871
<210> 9
<211> 4356
<212> DNA
<213> Artificial Sequence
<220>
<223> B_R0.3-mSA
<400> 9
gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga 60
taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact 120
cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga 180
actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 240
tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 300
gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat 360
caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg 420
tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 480
gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 540
tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 600
aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 660
cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 720
agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 780
ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 840
tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 900
tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 960
caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1020
accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1080
attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1140
ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1200
taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1260
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1320
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1380
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1440
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1500
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1560
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1620
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1680
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1740
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1800
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1860
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1920
acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1980
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2040
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2100
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2160
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2220
taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2280
cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2340
tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc 2400
atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 2460
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 2520
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 2580
aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc 2640
tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt 2700
gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa 2760
ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 2820
agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 2880
aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 2940
aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 3000
ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 3060
acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 3120
aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 3180
ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 3240
aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 3300
cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 3360
atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 3420
tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 3480
ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 3540
agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 3600
ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 3660
cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc 3720
attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 3780
cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 3840
tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 3900
tctccatacc cgttttttgg gctcacacag gaaaggctag catggcggaa gcgggtatca 3960
ccggcacgtg gtacaaccag catggttcta ccttcaccgt taccgcgggt gcggacggta 4020
acctgaccgg tcagtacgaa aaccgtgcgc agggcactgg ttgccagaac tctccgtaca 4080
ccctgaccgg tcgttacaac ggtaccaaac tggaatggcg tgttgaatgg aacaactcta 4140
ccgaaaactg ccactctcgt accgaatggc gtggtcagta ccagggtggt gcggaagcgc 4200
gtatcaacac ccagtggaac ctgacctacg aaggtggttc tggtccggcg accgaacagg 4260
gtcaggacac cttcaccaaa gttaaacacc accaccacca ccactaagtc gacctgcagg 4320
aagcttgttt aaacggtctc cagcttggct gttttg 4356
<210> 10
<211> 4358
<212> DNA
<213> Artificial Sequence
<220>
<223> B_R0.6-mSA
<400> 10
gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga 60
taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact 120
cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga 180
actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 240
tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 300
gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat 360
caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg 420
tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 480
gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 540
tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 600
aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 660
cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 720
agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 780
ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 840
tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 900
tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 960
caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1020
accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1080
attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1140
ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1200
taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1260
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1320
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1380
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1440
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1500
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1560
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1620
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1680
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1740
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1800
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1860
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1920
acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1980
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2040
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2100
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2160
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2220
taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2280
cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2340
tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc 2400
atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 2460
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 2520
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 2580
aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc 2640
tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt 2700
gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa 2760
ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 2820
agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 2880
aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 2940
aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 3000
ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 3060
acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 3120
aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 3180
ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 3240
aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 3300
cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 3360
atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 3420
tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 3480
ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 3540
agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 3600
ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 3660
cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc 3720
attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 3780
cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 3840
tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 3900
tctccatacc cgttttttgg gcattaaaga ggagaaagct agcatggcgg aagcgggtat 3960
caccggcacg tggtacaacc agcatggttc taccttcacc gttaccgcgg gtgcggacgg 4020
taacctgacc ggtcagtacg aaaaccgtgc gcagggcact ggttgccaga actctccgta 4080
caccctgacc ggtcgttaca acggtaccaa actggaatgg cgtgttgaat ggaacaactc 4140
taccgaaaac tgccactctc gtaccgaatg gcgtggtcag taccagggtg gtgcggaagc 4200
gcgtatcaac acccagtgga acctgaccta cgaaggtggt tctggtccgg cgaccgaaca 4260
gggtcaggac accttcacca aagttaaaca ccaccaccac caccactaag tcgacctgca 4320
ggaagcttgt ttaaacggtc tccagcttgg ctgttttg 4358
<210> 11
<211> 4355
<212> DNA
<213> Artificial Sequence
<220>
<223> B_R1.0-mSA
<400> 11
gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga 60
taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact 120
cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga 180
actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 240
tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 300
gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat 360
caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg 420
tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 480
gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 540
tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 600
aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 660
cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 720
agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 780
ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 840
tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 900
tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 960
caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1020
accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1080
attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1140
ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1200
taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1260
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1320
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1380
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1440
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1500
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1560
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1620
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1680
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1740
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1800
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1860
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1920
acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1980
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2040
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2100
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2160
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2220
taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2280
cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2340
tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc 2400
atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 2460
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 2520
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 2580
aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc 2640
tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt 2700
gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa 2760
ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 2820
agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 2880
aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 2940
aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 3000
ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 3060
acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 3120
aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 3180
ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 3240
aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 3300
cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 3360
atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 3420
tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 3480
ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 3540
agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 3600
ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 3660
cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc 3720
attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 3780
cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 3840
tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 3900
tctccatacc cgttttttgg gcaaagagga gaaagctagc atggcggaag cgggtatcac 3960
cggcacgtgg tacaaccagc atggttctac cttcaccgtt accgcgggtg cggacggtaa 4020
cctgaccggt cagtacgaaa accgtgcgca gggcactggt tgccagaact ctccgtacac 4080
cctgaccggt cgttacaacg gtaccaaact ggaatggcgt gttgaatgga acaactctac 4140
cgaaaactgc cactctcgta ccgaatggcg tggtcagtac cagggtggtg cggaagcgcg 4200
tatcaacacc cagtggaacc tgacctacga aggtggttct ggtccggcga ccgaacaggg 4260
tcaggacacc ttcaccaaag ttaaacacca ccaccaccac cactaagtcg acctgcagga 4320
agcttgttta aacggtctcc agcttggctg ttttg 4355
<210> 12
<211> 7057
<212> DNA
<213> Artificial Sequence
<220>
<223> M_p-mSA
<400> 12
ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga 60
gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg 120
gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa 180
cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac 240
aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc 300
acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg 360
tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc 420
ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca 480
ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga 540
cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc 600
tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg 660
cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag 720
cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga 780
atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa 840
tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg 900
acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc 960
gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga 1020
agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata 1080
cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt 1140
cccgactgga aagcgggcag tgagcgcaac gcaattaatg taagttagct cactcattag 1200
gcacaattct catgtttgac agcttatcat cgactgcacg gtgcaccaat gcttctggcg 1260
tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataattcg 1320
tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat cataacggtt 1380
ctggcaaata ttctgaaatg agctgttgac aattaatcat cggctcgtat aatgtgtgga 1440
attgtgagcg gataacaatt tcacacagga aacagccagt ccgtttaggt gttttcacga 1500
gcacttcacc aacaaggacc atagcatatg aaaataaaaa caggtgcacg catcctcgca 1560
ttatccgcat taacgacgat gatgttttcc gcctcggctc tcgccaaaat cgaagaaggt 1620
aaactggtaa tctggattaa cggcgataaa ggctataacg gtctcgctga agtcggtaag 1680
aaattcgaga aagataccgg aattaaagtc accgttgagc atccggataa actggaagag 1740
aaattcccac aggttgcggc aactggcgat ggccctgaca ttatcttctg ggcacacgac 1800
cgctttggtg gctacgctca atctggcctg ttggctgaaa tcaccccgga caaagcgttc 1860
caggacaagc tgtatccgtt tacctgggat gccgtacgtt acaacggcaa gctgattgct 1920
tacccgatcg ctgttgaagc gttatcgctg atttataaca aagatctgct gccgaacccg 1980
ccaaaaacct gggaagagat cccggcgctg gataaagaac tgaaagcgaa aggtaagagc 2040
gcgctgatgt tcaacctgca agaaccgtac ttcacctggc cgctgattgc tgctgacggg 2100
ggttatgcgt tcaagtatga aaacggcaag tacgacatta aagacgtggg cgtggataac 2160
gctggcgcga aagcgggtct gaccttcctg gttgacctga ttaaaaacaa acacatgaat 2220
gcagacaccg attactccat cgcagaagct gcctttaata aaggcgaaac agcgatgacc 2280
atcaacggcc cgtgggcatg gtccaacatc gacaccagca aagtgaatta tggtgtaacg 2340
gtactgccga ccttcaaggg tcaaccatcc aaaccgttcg ttggcgtgct gagcgcaggt 2400
attaacgccg ccagtccgaa caaagagctg gcaaaagagt tcctcgaaaa ctatctgctg 2460
actgatgaag gtctggaagc ggttaataaa gacaaaccgc tgggtgccgt agcgctgaag 2520
tcttacgagg aagagttggc gaaagatcca cgtattgccg ccactatgga aaacgcccag 2580
aaaggtgaaa tcatgccgaa catcccgcag atgtccgctt tctggtatgc cgtgcgtact 2640
gcggtgatca acgccgccag cggtcgtcag actgtcgatg aagccctgaa agacgcgcag 2700
actaattcga gctcgaacaa caacaacaat aacaataaca acaacctcgg gatcgaggga 2760
aggatttcag aattcgcgga agcgggtatc accggcacgt ggtacaacca gcatggttct 2820
accttcaccg ttaccgcggg tgcggacggt aacctgaccg gtcagtacga aaaccgtgcg 2880
cagggcactg gttgccagaa ctctccgtac accctgaccg gtcgttacaa cggtaccaaa 2940
ctggaatggc gtgttgaatg gaacaactct accgaaaact gccactctcg taccgaatgg 3000
cgtggtcagt accagggtgg tgcggaagcg cgtatcaaca cccagtggaa cctgacctac 3060
gaaggtggtt ctggtccggc gaccgaacag ggtcaggaca ccttcaccaa agttaaacac 3120
caccaccacc accactaaaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga 3180
aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg 3240
taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga 3300
atggcagctt ggctgttttg gcggatgaga taagattttc agcctgatac agattaaatc 3360
agaacgcaga agcggtctga taaaacagaa tttgcctggc ggcagtagcg cggtggtccc 3420
acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta gtgtggggtc 3480
tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct cagtcgaaag 3540
actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc 3600
cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc 3660
cgccataaac tgccaggcat caaattaagc agaaggccat cctgacggat ggcctttttg 3720
cgtttctaca aactcttttg tttatttttc taaatacatt caaatatgta tccgctcatg 3780
agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa 3840
catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac 3900
ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac 3960
atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttc 4020
ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc 4080
gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca 4140
ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc 4200
ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag 4260
gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa 4320
ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg 4380
gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa 4440
ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg 4500
gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt 4560
gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt 4620
caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag 4680
cattggtaac tgtcagacca agtttactca tatatacttt agattgattt accccggttg 4740
ataatcagaa aagccccaaa aacaggaaga ttgtataagc aaatatttaa attgtaaacg 4800
ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat 4860
aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg 4920
ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc 4980
gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcacccaaa tcaagttttt 5040
tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag 5100
cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg 5160
gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc 5220
ttaatgcgcc gctacagggc gcgtaaaagg atctaggtga agatcctttt tgataatctc 5280
atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag 5340
atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 5400
aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 5460
aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag 5520
ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 5580
ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 5640
tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 5700
ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc 5760
acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 5820
gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 5880
cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 5940
aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac 6000
atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga 6060
gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg 6120
gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata 6180
tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc 6240
gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc 6300
gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 6360
gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta 6420
aagctcatca gcgtggtcgt gcagcgattc acagatgtct gcctgttcat ccgcgtccag 6480
ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag 6540
ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg 6600
ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca 6660
tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca 6720
gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca 6780
gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt 6840
ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt 6900
cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg 6960
ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat 7020
gcgcacccgt ggccaggacc caacgctgcc cgaaatt 7057
<210> 13
<211> 6982
<212> DNA
<213> Artificial Sequence
<220>
<223> M_c-mSA
<400> 13
ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga 60
gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg 120
gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa 180
cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac 240
aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc 300
acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg 360
tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc 420
ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca 480
ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga 540
cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc 600
tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg 660
cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag 720
cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga 780
atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa 840
tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg 900
acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc 960
gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga 1020
agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata 1080
cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt 1140
cccgactgga aagcgggcag tgagcgcaac gcaattaatg taagttagct cactcattag 1200
gcacaattct catgtttgac agcttatcat cgactgcacg gtgcaccaat gcttctggcg 1260
tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataattcg 1320
tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat cataacggtt 1380
ctggcaaata ttctgaaatg agctgttgac aattaatcat cggctcgtat aatgtgtgga 1440
attgtgagcg gataacaatt tcacacagga aacagccagt ccgtttaggt gttttcacga 1500
gcacttcacc aacaaggacc atagcatatg aaaatcgaag aaggtaaact ggtaatctgg 1560
attaacggcg ataaaggcta taacggtctc gctgaagtcg gtaagaaatt cgagaaagat 1620
accggaatta aagtcaccgt tgagcatccg gataaactgg aagagaaatt cccacaggtt 1680
gcggcaactg gcgatggccc tgacattatc ttctgggcac acgaccgctt tggtggctac 1740
gctcaatctg gcctgttggc tgaaatcacc ccggacaaag cgttccagga caagctgtat 1800
ccgtttacct gggatgccgt acgttacaac ggcaagctga ttgcttaccc gatcgctgtt 1860
gaagcgttat cgctgattta taacaaagat ctgctgccga acccgccaaa aacctgggaa 1920
gagatcccgg cgctggataa agaactgaaa gcgaaaggta agagcgcgct gatgttcaac 1980
ctgcaagaac cgtacttcac ctggccgctg attgctgctg acgggggtta tgcgttcaag 2040
tatgaaaacg gcaagtacga cattaaagac gtgggcgtgg ataacgctgg cgcgaaagcg 2100
ggtctgacct tcctggttga cctgattaaa aacaaacaca tgaatgcaga caccgattac 2160
tccatcgcag aagctgcctt taataaaggc gaaacagcga tgaccatcaa cggcccgtgg 2220
gcatggtcca acatcgacac cagcaaagtg aattatggtg taacggtact gccgaccttc 2280
aagggtcaac catccaaacc gttcgttggc gtgctgagcg caggtattaa cgccgccagt 2340
ccgaacaaag agctggcaaa agagttcctc gaaaactatc tgctgactga tgaaggtctg 2400
gaagcggtta ataaagacaa accgctgggt gccgtagcgc tgaagtctta cgaggaagag 2460
ttggcgaaag atccacgtat tgccgccact atggaaaacg cccagaaagg tgaaatcatg 2520
ccgaacatcc cgcagatgtc cgctttctgg tatgccgtgc gtactgcggt gatcaacgcc 2580
gccagcggtc gtcagactgt cgatgaagcc ctgaaagacg cgcagactaa ttcgagctcg 2640
aacaacaaca acaataacaa taacaacaac ctcgggatcg agggaaggat ttcagaattc 2700
gcggaagcgg gtatcaccgg cacgtggtac aaccagcatg gttctacctt caccgttacc 2760
gcgggtgcgg acggtaacct gaccggtcag tacgaaaacc gtgcgcaggg cactggttgc 2820
cagaactctc cgtacaccct gaccggtcgt tacaacggta ccaaactgga atggcgtgtt 2880
gaatggaaca actctaccga aaactgccac tctcgtaccg aatggcgtgg tcagtaccag 2940
ggtggtgcgg aagcgcgtat caacacccag tggaacctga cctacgaagg tggttctggt 3000
ccggcgaccg aacagggtca ggacaccttc accaaagtta aacaccacca ccaccaccac 3060
taaaagcttg gcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac 3120
ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc 3180
ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc agcttggctg 3240
ttttggcgga tgagataaga ttttcagcct gatacagatt aaatcagaac gcagaagcgg 3300
tctgataaaa cagaatttgc ctggcggcag tagcgcggtg gtcccacctg accccatgcc 3360
gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg gggtctcccc atgcgagagt 3420
agggaactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 3480
ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ggagcggatt 3540
tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg acgcccgcca taaactgcca 3600
ggcatcaaat taagcagaag gccatcctga cggatggcct ttttgcgttt ctacaaactc 3660
ttttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga 3720
taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc 3780
cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg 3840
aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc 3900
aacagcggta agatccttga gagttttcgc cccgaagaac gtttcccaat gatgagcact 3960
tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc 4020
ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag 4080
catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat 4140
aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt 4200
ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa 4260
gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc 4320
aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg 4380
gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt 4440
gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca 4500
gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat 4560
gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca 4620
gaccaagttt actcatatat actttagatt gatttacccc ggttgataat cagaaaagcc 4680
ccaaaaacag gaagattgta taagcaaata tttaaattgt aaacgttaat attttgttaa 4740
aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca 4800
aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga 4860
acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc 4920
agggcgatgg cccactacgt gaaccatcac ccaaatcaag ttttttgggg tcgaggtgcc 4980
gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc 5040
cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg 5100
caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac 5160
agggcgcgta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct 5220
taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 5280
tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 5340
gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 5400
agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc 5460
aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 5520
gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 5580
gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 5640
tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg 5700
agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 5760
cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 5820
gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 5880
gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg 5940
ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc 6000
cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg 6060
cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca 6120
gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga 6180
ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg 6240
tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca 6300
gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg 6360
gtcgtgcagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc 6420
cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg 6480
tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat 6540
gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga 6600
acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca 6660
gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca 6720
tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact 6780
ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca 6840
gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca 6900
accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca 6960
ggacccaacg ctgcccgaaa tt 6982
<210> 14
<211> 5606
<212> DNA
<213> Artificial Sequence
<220>
<223> B_p-mSA
<400> 14
gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga 60
taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact 120
cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga 180
actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 240
tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 300
gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat 360
caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg 420
tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 480
gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 540
tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 600
aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 660
cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 720
agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 780
ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 840
tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 900
tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 960
caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1020
accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1080
attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1140
ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1200
taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1260
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1320
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1380
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1440
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1500
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1560
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1620
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1680
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1740
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1800
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1860
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1920
acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1980
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2040
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2100
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2160
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2220
taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2280
cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2340
tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc 2400
atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 2460
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 2520
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 2580
aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc 2640
tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt 2700
gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa 2760
ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 2820
agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 2880
aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 2940
aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 3000
ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 3060
acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 3120
aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 3180
ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 3240
aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 3300
cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 3360
atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 3420
tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 3480
ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 3540
agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 3600
ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 3660
cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc 3720
attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 3780
cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 3840
tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 3900
tctccatacc cgttttttgg gctaacagga ggaagctagc gctagcatga aaataaaaac 3960
aggtgcacgc atcctcgcat tatccgcatt aacgacgatg atgttttccg cctcggctct 4020
cgccaaaatc gaagaaggta aactggtaat ctggattaac ggcgataaag gctataacgg 4080
tctcgctgaa gtcggtaaga aattcgagaa agataccgga attaaagtca ccgttgagca 4140
tccggataaa ctggaagaga aattcccaca ggttgcggca actggcgatg gccctgacat 4200
tatcttctgg gcacacgacc gctttggtgg ctacgctcaa tctggcctgt tggctgaaat 4260
caccccggac aaagcgttcc aggacaagct gtatccgttt acctgggatg ccgtacgtta 4320
caacggcaag ctgattgctt acccgatcgc tgttgaagcg ttatcgctga tttataacaa 4380
agatctgctg ccgaacccgc caaaaacctg ggaagagatc ccggcgctgg ataaagaact 4440
gaaagcgaaa ggtaagagcg cgctgatgtt caacctgcaa gaaccgtact tcacctggcc 4500
gctgattgct gctgacgggg gttatgcgtt caagtatgaa aacggcaagt acgacattaa 4560
agacgtgggc gtggataacg ctggcgcgaa agcgggtctg accttcctgg ttgacctgat 4620
taaaaacaaa cacatgaatg cagacaccga ttactccatc gcagaagctg cctttaataa 4680
aggcgaaaca gcgatgacca tcaacggccc gtgggcatgg tccaacatcg acaccagcaa 4740
agtgaattat ggtgtaacgg tactgccgac cttcaagggt caaccatcca aaccgttcgt 4800
tggcgtgctg agcgcaggta ttaacgccgc cagtccgaac aaagagctgg caaaagagtt 4860
cctcgaaaac tatctgctga ctgatgaagg tctggaagcg gttaataaag acaaaccgct 4920
gggtgccgta gcgctgaagt cttacgagga agagttggcg aaagatccac gtattgccgc 4980
caccatggaa aacgcccaga aaggtgaaat catgccgaac atcccgcaga tgtccgcttt 5040
ctggtatgcc gtgcgtactg cggtgatcaa cgccgccagc ggtcgtcaga ctgtcgatga 5100
agccctgaaa gacgcgcaga ctaattcgag ctcgaacaac aacaacaata acaataacaa 5160
caacctcggg atcgagggaa ggatttcaga attcgcggaa gcgggtatca ccggcacgtg 5220
gtacaaccag catggttcta ccttcaccgt taccgcgggt gcggacggta acctgaccgg 5280
tcagtacgaa aaccgtgcgc agggcactgg ttgccagaac tctccgtaca ccctgaccgg 5340
tcgttacaac ggtaccaaac tggaatggcg tgttgaatgg aacaactcta ccgaaaactg 5400
ccactctcgt accgaatggc gtggtcagta ccagggtggt gcggaagcgc gtatcaacac 5460
ccagtggaac ctgacctacg aaggtggttc tggtccggcg accgaacagg gtcaggacac 5520
cttcaccaaa gttaaacacc accaccacca ccactaagtc gacctgcagg aagcttgttt 5580
aaacggtctc cagcttggct gttttg 5606
<210> 15
<211> 5531
<212> DNA
<213> Artificial Sequence
<220>
<223> B_c-mSA
<400> 15
gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga 60
taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact 120
cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga 180
actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 240
tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 300
gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat 360
caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg 420
tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 480
gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 540
tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 600
aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 660
cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 720
agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 780
ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 840
tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 900
tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 960
caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1020
accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1080
attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1140
ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1200
taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1260
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1320
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1380
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1440
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1500
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1560
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1620
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1680
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1740
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1800
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1860
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1920
acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1980
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2040
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2100
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2160
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2220
taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2280
cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2340
tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc 2400
atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 2460
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 2520
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 2580
aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc 2640
tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt 2700
gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa 2760
ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 2820
agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 2880
aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 2940
aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 3000
ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 3060
acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 3120
aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 3180
ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 3240
aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 3300
cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 3360
atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 3420
tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 3480
ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 3540
agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 3600
ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 3660
cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc 3720
attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 3780
cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 3840
tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 3900
tctccatacc cgttttttgg gctaacagga ggaagctagc gctagcatga aaatcgaaga 3960
aggtaaactg gtaatctgga ttaacggcga taaaggctat aacggtctcg ctgaagtcgg 4020
taagaaattc gagaaagata ccggaattaa agtcaccgtt gagcatccgg ataaactgga 4080
agagaaattc ccacaggttg cggcaactgg cgatggccct gacattatct tctgggcaca 4140
cgaccgcttt ggtggctacg ctcaatctgg cctgttggct gaaatcaccc cggacaaagc 4200
gttccaggac aagctgtatc cgtttacctg ggatgccgta cgttacaacg gcaagctgat 4260
tgcttacccg atcgctgttg aagcgttatc gctgatttat aacaaagatc tgctgccgaa 4320
cccgccaaaa acctgggaag agatcccggc gctggataaa gaactgaaag cgaaaggtaa 4380
gagcgcgctg atgttcaacc tgcaagaacc gtacttcacc tggccgctga ttgctgctga 4440
cgggggttat gcgttcaagt atgaaaacgg caagtacgac attaaagacg tgggcgtgga 4500
taacgctggc gcgaaagcgg gtctgacctt cctggttgac ctgattaaaa acaaacacat 4560
gaatgcagac accgattact ccatcgcaga agctgccttt aataaaggcg aaacagcgat 4620
gaccatcaac ggcccgtggg catggtccaa catcgacacc agcaaagtga attatggtgt 4680
aacggtactg ccgaccttca agggtcaacc atccaaaccg ttcgttggcg tgctgagcgc 4740
aggtattaac gccgccagtc cgaacaaaga gctggcaaaa gagttcctcg aaaactatct 4800
gctgactgat gaaggtctgg aagcggttaa taaagacaaa ccgctgggtg ccgtagcgct 4860
gaagtcttac gaggaagagt tggcgaaaga tccacgtatt gccgccacca tggaaaacgc 4920
ccagaaaggt gaaatcatgc cgaacatccc gcagatgtcc gctttctggt atgccgtgcg 4980
tactgcggtg atcaacgccg ccagcggtcg tcagactgtc gatgaagccc tgaaagacgc 5040
gcagactaat tcgagctcga acaacaacaa caataacaat aacaacaacc tcgggatcga 5100
gggaaggatt tcagaattcg cggaagcggg tatcaccggc acgtggtaca accagcatgg 5160
ttctaccttc accgttaccg cgggtgcgga cggtaacctg accggtcagt acgaaaaccg 5220
tgcgcagggc actggttgcc agaactctcc gtacaccctg accggtcgtt acaacggtac 5280
caaactggaa tggcgtgttg aatggaacaa ctctaccgaa aactgccact ctcgtaccga 5340
atggcgtggt cagtaccagg gtggtgcgga agcgcgtatc aacacccagt ggaacctgac 5400
ctacgaaggt ggttctggtc cggcgaccga acagggtcag gacaccttca ccaaagttaa 5460
acaccaccac caccaccact aagtcgacct gcaggaagct tgtttaaacg gtctccagct 5520
tggctgtttt g 5531
<210> 16
<211> 5600
<212> DNA
<213> Artificial Sequence
<220>
<223> B_R1.0-p-mSA
<400> 16
gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga 60
taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact 120
cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga 180
actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 240
tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 300
gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat 360
caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg 420
tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 480
gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 540
tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 600
aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 660
cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 720
agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 780
ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 840
tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 900
tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 960
caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1020
accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1080
attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1140
ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1200
taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1260
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1320
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1380
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1440
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1500
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1560
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1620
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1680
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1740
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1800
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1860
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1920
acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1980
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2040
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2100
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2160
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2220
taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2280
cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2340
tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc 2400
atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 2460
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 2520
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 2580
aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc 2640
tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt 2700
gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa 2760
ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 2820
agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 2880
aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 2940
aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 3000
ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 3060
acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 3120
aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 3180
ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 3240
aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 3300
cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 3360
atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 3420
tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 3480
ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 3540
agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 3600
ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 3660
cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc 3720
attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 3780
cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 3840
tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 3900
tctccatacc cgttttttgg gcaaagagga gaaagctagc atgaaaataa aaacaggtgc 3960
acgcatcctc gcattatccg cattaacgac gatgatgttt tccgcctcgg ctctcgccaa 4020
aatcgaagaa ggtaaactgg taatctggat taacggcgat aaaggctata acggtctcgc 4080
tgaagtcggt aagaaattcg agaaagatac cggaattaaa gtcaccgttg agcatccgga 4140
taaactggaa gagaaattcc cacaggttgc ggcaactggc gatggccctg acattatctt 4200
ctgggcacac gaccgctttg gtggctacgc tcaatctggc ctgttggctg aaatcacccc 4260
ggacaaagcg ttccaggaca agctgtatcc gtttacctgg gatgccgtac gttacaacgg 4320
caagctgatt gcttacccga tcgctgttga agcgttatcg ctgatttata acaaagatct 4380
gctgccgaac ccgccaaaaa cctgggaaga gatcccggcg ctggataaag aactgaaagc 4440
gaaaggtaag agcgcgctga tgttcaacct gcaagaaccg tacttcacct ggccgctgat 4500
tgctgctgac gggggttatg cgttcaagta tgaaaacggc aagtacgaca ttaaagacgt 4560
gggcgtggat aacgctggcg cgaaagcggg tctgaccttc ctggttgacc tgattaaaaa 4620
caaacacatg aatgcagaca ccgattactc catcgcagaa gctgccttta ataaaggcga 4680
aacagcgatg accatcaacg gcccgtgggc atggtccaac atcgacacca gcaaagtgaa 4740
ttatggtgta acggtactgc cgaccttcaa gggtcaacca tccaaaccgt tcgttggcgt 4800
gctgagcgca ggtattaacg ccgccagtcc gaacaaagag ctggcaaaag agttcctcga 4860
aaactatctg ctgactgatg aaggtctgga agcggttaat aaagacaaac cgctgggtgc 4920
cgtagcgctg aagtcttacg aggaagagtt ggcgaaagat ccacgtattg ccgccaccat 4980
ggaaaacgcc cagaaaggtg aaatcatgcc gaacatcccg cagatgtccg ctttctggta 5040
tgccgtgcgt actgcggtga tcaacgccgc cagcggtcgt cagactgtcg atgaagccct 5100
gaaagacgcg cagactaatt cgagctcgaa caacaacaac aataacaata acaacaacct 5160
cgggatcgag ggaaggattt cagaattcgc ggaagcgggt atcaccggca cgtggtacaa 5220
ccagcatggt tctaccttca ccgttaccgc gggtgcggac ggtaacctga ccggtcagta 5280
cgaaaaccgt gcgcagggca ctggttgcca gaactctccg tacaccctga ccggtcgtta 5340
caacggtacc aaactggaat ggcgtgttga atggaacaac tctaccgaaa actgccactc 5400
tcgtaccgaa tggcgtggtc agtaccaggg tggtgcggaa gcgcgtatca acacccagtg 5460
gaacctgacc tacgaaggtg gttctggtcc ggcgaccgaa cagggtcagg acaccttcac 5520
caaagttaaa caccaccacc accaccacta agtcgacctg caggaagctt gtttaaacgg 5580
tctccagctt ggctgttttg 5600
<210> 17
<211> 5525
<212> DNA
<213> Artificial Sequence
<220>
<223> B_R1.0-c-mSA
<400> 17
gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga 60
taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact 120
cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga 180
actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 240
tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 300
gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat 360
caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg 420
tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 480
gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 540
tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 600
aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 660
cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 720
agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 780
ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 840
tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 900
tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 960
caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1020
accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1080
attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1140
ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1200
taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1260
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1320
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1380
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1440
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1500
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1560
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1620
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1680
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1740
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1800
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1860
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1920
acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1980
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2040
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2100
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2160
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2220
taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2280
cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2340
tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc 2400
atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 2460
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 2520
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 2580
aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc 2640
tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt 2700
gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa 2760
ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 2820
agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 2880
aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 2940
aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 3000
ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 3060
acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 3120
aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 3180
ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 3240
aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 3300
cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 3360
atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 3420
tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 3480
ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 3540
agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 3600
ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 3660
cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc 3720
attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 3780
cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 3840
tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 3900
tctccatacc cgttttttgg gcaaagagga gaaagctagc atgaaaatcg aagaaggtaa 3960
actggtaatc tggattaacg gcgataaagg ctataacggt ctcgctgaag tcggtaagaa 4020
attcgagaaa gataccggaa ttaaagtcac cgttgagcat ccggataaac tggaagagaa 4080
attcccacag gttgcggcaa ctggcgatgg ccctgacatt atcttctggg cacacgaccg 4140
ctttggtggc tacgctcaat ctggcctgtt ggctgaaatc accccggaca aagcgttcca 4200
ggacaagctg tatccgttta cctgggatgc cgtacgttac aacggcaagc tgattgctta 4260
cccgatcgct gttgaagcgt tatcgctgat ttataacaaa gatctgctgc cgaacccgcc 4320
aaaaacctgg gaagagatcc cggcgctgga taaagaactg aaagcgaaag gtaagagcgc 4380
gctgatgttc aacctgcaag aaccgtactt cacctggccg ctgattgctg ctgacggggg 4440
ttatgcgttc aagtatgaaa acggcaagta cgacattaaa gacgtgggcg tggataacgc 4500
tggcgcgaaa gcgggtctga ccttcctggt tgacctgatt aaaaacaaac acatgaatgc 4560
agacaccgat tactccatcg cagaagctgc ctttaataaa ggcgaaacag cgatgaccat 4620
caacggcccg tgggcatggt ccaacatcga caccagcaaa gtgaattatg gtgtaacggt 4680
actgccgacc ttcaagggtc aaccatccaa accgttcgtt ggcgtgctga gcgcaggtat 4740
taacgccgcc agtccgaaca aagagctggc aaaagagttc ctcgaaaact atctgctgac 4800
tgatgaaggt ctggaagcgg ttaataaaga caaaccgctg ggtgccgtag cgctgaagtc 4860
ttacgaggaa gagttggcga aagatccacg tattgccgcc accatggaaa acgcccagaa 4920
aggtgaaatc atgccgaaca tcccgcagat gtccgctttc tggtatgccg tgcgtactgc 4980
ggtgatcaac gccgccagcg gtcgtcagac tgtcgatgaa gccctgaaag acgcgcagac 5040
taattcgagc tcgaacaaca acaacaataa caataacaac aacctcggga tcgagggaag 5100
gatttcagaa ttcgcggaag cgggtatcac cggcacgtgg tacaaccagc atggttctac 5160
cttcaccgtt accgcgggtg cggacggtaa cctgaccggt cagtacgaaa accgtgcgca 5220
gggcactggt tgccagaact ctccgtacac cctgaccggt cgttacaacg gtaccaaact 5280
ggaatggcgt gttgaatgga acaactctac cgaaaactgc cactctcgta ccgaatggcg 5340
tggtcagtac cagggtggtg cggaagcgcg tatcaacacc cagtggaacc tgacctacga 5400
aggtggttct ggtccggcga ccgaacaggg tcaggacacc ttcaccaaag ttaaacacca 5460
ccaccaccac cactaagtcg acctgcagga agcttgttta aacggtctcc agcttggctg 5520
ttttg 5525
<210> 18
<211> 5594
<212> DNA
<213> Artificial Sequence
<220>
<223> B_R01-p-mSA
<400> 18
gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga 60
taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact 120
cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga 180
actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 240
tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 300
gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat 360
caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg 420
tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 480
gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 540
tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 600
aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 660
cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 720
agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 780
ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 840
tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 900
tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 960
caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1020
accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1080
attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1140
ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1200
taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1260
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1320
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1380
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1440
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1500
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1560
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1620
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1680
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1740
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1800
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1860
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1920
acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1980
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2040
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2100
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2160
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2220
taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2280
cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2340
tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc 2400
atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 2460
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 2520
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 2580
aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc 2640
tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt 2700
gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa 2760
ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 2820
agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 2880
aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 2940
aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 3000
ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 3060
acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 3120
aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 3180
ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 3240
aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 3300
cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 3360
atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 3420
tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 3480
ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 3540
agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 3600
ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 3660
cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc 3720
attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 3780
cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 3840
tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 3900
tctccattag cactcgttga catacggacg tcacatgaaa ataaaaacag gtgcacgcat 3960
cctcgcatta tccgcattaa cgacgatgat gttttccgcc tcggctctcg ccaaaatcga 4020
agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt 4080
cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact 4140
ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc 4200
acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa 4260
agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct 4320
gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc 4380
gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg 4440
taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc 4500
tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt 4560
ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca 4620
catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc 4680
gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg 4740
tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag 4800
cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta 4860
tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc 4920
gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ccatggaaaa 4980
cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt 5040
gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga 5100
cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat 5160
cgagggaagg atttcagaat tcgcggaagc gggtatcacc ggcacgtggt acaaccagca 5220
tggttctacc ttcaccgtta ccgcgggtgc ggacggtaac ctgaccggtc agtacgaaaa 5280
ccgtgcgcag ggcactggtt gccagaactc tccgtacacc ctgaccggtc gttacaacgg 5340
taccaaactg gaatggcgtg ttgaatggaa caactctacc gaaaactgcc actctcgtac 5400
cgaatggcgt ggtcagtacc agggtggtgc ggaagcgcgt atcaacaccc agtggaacct 5460
gacctacgaa ggtggttctg gtccggcgac cgaacagggt caggacacct tcaccaaagt 5520
taaacaccac caccaccacc actaagtcga cctgcaggaa gcttgtttaa acggtctcca 5580
gcttggctgt tttg 5594
<210> 19
<211> 5582
<212> DNA
<213> Artificial Sequence
<220>
<223> B_R02-p-mSA
<400> 19
gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga 60
taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact 120
cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga 180
actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 240
tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 300
gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat 360
caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg 420
tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 480
gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 540
tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 600
aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 660
cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 720
agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 780
ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 840
tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 900
tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 960
caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1020
accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1080
attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1140
ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1200
taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1260
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1320
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1380
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1440
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1500
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1560
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1620
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1680
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1740
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1800
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1860
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1920
acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1980
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2040
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2100
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2160
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2220
taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2280
cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2340
tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc 2400
atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 2460
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 2520
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 2580
aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc 2640
tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt 2700
gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa 2760
ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 2820
agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 2880
aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 2940
aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 3000
ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 3060
acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 3120
aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 3180
ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 3240
aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 3300
cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 3360
atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 3420
tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 3480
ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 3540
agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 3600
ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 3660
cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc 3720
attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 3780
cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 3840
tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 3900
tctccatact actgaggcta ctatgaaaat aaaaacaggt gcacgcatcc tcgcattatc 3960
cgcattaacg acgatgatgt tttccgcctc ggctctcgcc aaaatcgaag aaggtaaact 4020
ggtaatctgg attaacggcg ataaaggcta taacggtctc gctgaagtcg gtaagaaatt 4080
cgagaaagat accggaatta aagtcaccgt tgagcatccg gataaactgg aagagaaatt 4140
cccacaggtt gcggcaactg gcgatggccc tgacattatc ttctgggcac acgaccgctt 4200
tggtggctac gctcaatctg gcctgttggc tgaaatcacc ccggacaaag cgttccagga 4260
caagctgtat ccgtttacct gggatgccgt acgttacaac ggcaagctga ttgcttaccc 4320
gatcgctgtt gaagcgttat cgctgattta taacaaagat ctgctgccga acccgccaaa 4380
aacctgggaa gagatcccgg cgctggataa agaactgaaa gcgaaaggta agagcgcgct 4440
gatgttcaac ctgcaagaac cgtacttcac ctggccgctg attgctgctg acgggggtta 4500
tgcgttcaag tatgaaaacg gcaagtacga cattaaagac gtgggcgtgg ataacgctgg 4560
cgcgaaagcg ggtctgacct tcctggttga cctgattaaa aacaaacaca tgaatgcaga 4620
caccgattac tccatcgcag aagctgcctt taataaaggc gaaacagcga tgaccatcaa 4680
cggcccgtgg gcatggtcca acatcgacac cagcaaagtg aattatggtg taacggtact 4740
gccgaccttc aagggtcaac catccaaacc gttcgttggc gtgctgagcg caggtattaa 4800
cgccgccagt ccgaacaaag agctggcaaa agagttcctc gaaaactatc tgctgactga 4860
tgaaggtctg gaagcggtta ataaagacaa accgctgggt gccgtagcgc tgaagtctta 4920
cgaggaagag ttggcgaaag atccacgtat tgccgccacc atggaaaacg cccagaaagg 4980
tgaaatcatg ccgaacatcc cgcagatgtc cgctttctgg tatgccgtgc gtactgcggt 5040
gatcaacgcc gccagcggtc gtcagactgt cgatgaagcc ctgaaagacg cgcagactaa 5100
ttcgagctcg aacaacaaca acaataacaa taacaacaac ctcgggatcg agggaaggat 5160
ttcagaattc gcggaagcgg gtatcaccgg cacgtggtac aaccagcatg gttctacctt 5220
caccgttacc gcgggtgcgg acggtaacct gaccggtcag tacgaaaacc gtgcgcaggg 5280
cactggttgc cagaactctc cgtacaccct gaccggtcgt tacaacggta ccaaactgga 5340
atggcgtgtt gaatggaaca actctaccga aaactgccac tctcgtaccg aatggcgtgg 5400
tcagtaccag ggtggtgcgg aagcgcgtat caacacccag tggaacctga cctacgaagg 5460
tggttctggt ccggcgaccg aacagggtca ggacaccttc accaaagtta aacaccacca 5520
ccaccaccac taagtcgacc tgcaggaagc ttgtttaaac ggtctccagc ttggctgttt 5580
tg 5582
<210> 20
<211> 5597
<212> DNA
<213> Artificial Sequence
<220>
<223> B_R1-p-mSA
<400> 20
gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga 60
taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact 120
cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga 180
actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 240
tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 300
gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat 360
caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg 420
tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 480
gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 540
tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 600
aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 660
cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 720
agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 780
ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 840
tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 900
tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 960
caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1020
accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1080
attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1140
ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1200
taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1260
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1320
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1380
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1440
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1500
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1560
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1620
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1680
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1740
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1800
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1860
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1920
acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1980
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2040
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2100
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2160
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2220
taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2280
cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2340
tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc 2400
atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 2460
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 2520
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 2580
aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc 2640
tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt 2700
gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa 2760
ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 2820
agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 2880
aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 2940
aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 3000
ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 3060
acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 3120
aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 3180
ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 3240
aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 3300
cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 3360
atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 3420
tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 3480
ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 3540
agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 3600
ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 3660
cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc 3720
attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 3780
cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 3840
tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 3900
tctccattgg aacagctcac gcaaaaatag gtttcttatg aaaataaaaa caggtgcacg 3960
catcctcgca ttatccgcat taacgacgat gatgttttcc gcctcggctc tcgccaaaat 4020
cgaagaaggt aaactggtaa tctggattaa cggcgataaa ggctataacg gtctcgctga 4080
agtcggtaag aaattcgaga aagataccgg aattaaagtc accgttgagc atccggataa 4140
actggaagag aaattcccac aggttgcggc aactggcgat ggccctgaca ttatcttctg 4200
ggcacacgac cgctttggtg gctacgctca atctggcctg ttggctgaaa tcaccccgga 4260
caaagcgttc caggacaagc tgtatccgtt tacctgggat gccgtacgtt acaacggcaa 4320
gctgattgct tacccgatcg ctgttgaagc gttatcgctg atttataaca aagatctgct 4380
gccgaacccg ccaaaaacct gggaagagat cccggcgctg gataaagaac tgaaagcgaa 4440
aggtaagagc gcgctgatgt tcaacctgca agaaccgtac ttcacctggc cgctgattgc 4500
tgctgacggg ggttatgcgt tcaagtatga aaacggcaag tacgacatta aagacgtggg 4560
cgtggataac gctggcgcga aagcgggtct gaccttcctg gttgacctga ttaaaaacaa 4620
acacatgaat gcagacaccg attactccat cgcagaagct gcctttaata aaggcgaaac 4680
agcgatgacc atcaacggcc cgtgggcatg gtccaacatc gacaccagca aagtgaatta 4740
tggtgtaacg gtactgccga ccttcaaggg tcaaccatcc aaaccgttcg ttggcgtgct 4800
gagcgcaggt attaacgccg ccagtccgaa caaagagctg gcaaaagagt tcctcgaaaa 4860
ctatctgctg actgatgaag gtctggaagc ggttaataaa gacaaaccgc tgggtgccgt 4920
agcgctgaag tcttacgagg aagagttggc gaaagatcca cgtattgccg ccaccatgga 4980
aaacgcccag aaaggtgaaa tcatgccgaa catcccgcag atgtccgctt tctggtatgc 5040
cgtgcgtact gcggtgatca acgccgccag cggtcgtcag actgtcgatg aagccctgaa 5100
agacgcgcag actaattcga gctcgaacaa caacaacaat aacaataaca acaacctcgg 5160
gatcgaggga aggatttcag aattcgcgga agcgggtatc accggcacgt ggtacaacca 5220
gcatggttct accttcaccg ttaccgcggg tgcggacggt aacctgaccg gtcagtacga 5280
aaaccgtgcg cagggcactg gttgccagaa ctctccgtac accctgaccg gtcgttacaa 5340
cggtaccaaa ctggaatggc gtgttgaatg gaacaactct accgaaaact gccactctcg 5400
taccgaatgg cgtggtcagt accagggtgg tgcggaagcg cgtatcaaca cccagtggaa 5460
cctgacctac gaaggtggtt ctggtccggc gaccgaacag ggtcaggaca ccttcaccaa 5520
agttaaacac caccaccacc accactaagt cgacctgcag gaagcttgtt taaacggtct 5580
ccagcttggc tgttttg 5597
<210> 21
<211> 5601
<212> DNA
<213> Artificial Sequence
<220>
<223> B_R11-p-mSA
<400> 21
gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga 60
taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact 120
cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga 180
actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 240
tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 300
gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat 360
caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg 420
tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 480
gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 540
tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 600
aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 660
cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 720
agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 780
ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 840
tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 900
tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 960
caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1020
accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1080
attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1140
ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1200
taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1260
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1320
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1380
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1440
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1500
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1560
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1620
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1680
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1740
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1800
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1860
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1920
acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1980
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2040
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2100
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2160
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2220
taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2280
cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2340
tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc 2400
atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 2460
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 2520
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 2580
aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc 2640
tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt 2700
gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa 2760
ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 2820
agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 2880
aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 2940
aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 3000
ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 3060
acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 3120
aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 3180
ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 3240
aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 3300
cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 3360
atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 3420
tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 3480
ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 3540
agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 3600
ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 3660
cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc 3720
attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 3780
cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 3840
tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 3900
tctccatcgc tttttatcgc aactctctac tgtttctcca tatgaaaata aaaacaggtg 3960
cacgcatcct cgcattatcc gcattaacga cgatgatgtt ttccgcctcg gctctcgcca 4020
aaatcgaaga aggtaaactg gtaatctgga ttaacggcga taaaggctat aacggtctcg 4080
ctgaagtcgg taagaaattc gagaaagata ccggaattaa agtcaccgtt gagcatccgg 4140
ataaactgga agagaaattc ccacaggttg cggcaactgg cgatggccct gacattatct 4200
tctgggcaca cgaccgcttt ggtggctacg ctcaatctgg cctgttggct gaaatcaccc 4260
cggacaaagc gttccaggac aagctgtatc cgtttacctg ggatgccgta cgttacaacg 4320
gcaagctgat tgcttacccg atcgctgttg aagcgttatc gctgatttat aacaaagatc 4380
tgctgccgaa cccgccaaaa acctgggaag agatcccggc gctggataaa gaactgaaag 4440
cgaaaggtaa gagcgcgctg atgttcaacc tgcaagaacc gtacttcacc tggccgctga 4500
ttgctgctga cgggggttat gcgttcaagt atgaaaacgg caagtacgac attaaagacg 4560
tgggcgtgga taacgctggc gcgaaagcgg gtctgacctt cctggttgac ctgattaaaa 4620
acaaacacat gaatgcagac accgattact ccatcgcaga agctgccttt aataaaggcg 4680
aaacagcgat gaccatcaac ggcccgtggg catggtccaa catcgacacc agcaaagtga 4740
attatggtgt aacggtactg ccgaccttca agggtcaacc atccaaaccg ttcgttggcg 4800
tgctgagcgc aggtattaac gccgccagtc cgaacaaaga gctggcaaaa gagttcctcg 4860
aaaactatct gctgactgat gaaggtctgg aagcggttaa taaagacaaa ccgctgggtg 4920
ccgtagcgct gaagtcttac gaggaagagt tggcgaaaga tccacgtatt gccgccacca 4980
tggaaaacgc ccagaaaggt gaaatcatgc cgaacatccc gcagatgtcc gctttctggt 5040
atgccgtgcg tactgcggtg atcaacgccg ccagcggtcg tcagactgtc gatgaagccc 5100
tgaaagacgc gcagactaat tcgagctcga acaacaacaa caataacaat aacaacaacc 5160
tcgggatcga gggaaggatt tcagaattcg cggaagcggg tatcaccggc acgtggtaca 5220
accagcatgg ttctaccttc accgttaccg cgggtgcgga cggtaacctg accggtcagt 5280
acgaaaaccg tgcgcagggc actggttgcc agaactctcc gtacaccctg accggtcgtt 5340
acaacggtac caaactggaa tggcgtgttg aatggaacaa ctctaccgaa aactgccact 5400
ctcgtaccga atggcgtggt cagtaccagg gtggtgcgga agcgcgtatc aacacccagt 5460
ggaacctgac ctacgaaggt ggttctggtc cggcgaccga acagggtcag gacaccttca 5520
ccaaagttaa acaccaccac caccaccact aagtcgacct gcaggaagct tgtttaaacg 5580
gtctccagct tggctgtttt g 5601
<210> 22
<211> 5592
<212> DNA
<213> Artificial Sequence
<220>
<223> B_R12-p-mSA
<400> 22
gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga 60
taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact 120
cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga 180
actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 240
tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 300
gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat 360
caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg 420
tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 480
gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 540
tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 600
aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 660
cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 720
agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 780
ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 840
tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 900
tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 960
caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1020
accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1080
attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1140
ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1200
taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1260
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1320
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1380
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1440
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1500
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1560
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1620
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1680
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1740
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1800
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1860
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1920
acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1980
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2040
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2100
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2160
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2220
taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2280
cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2340
tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc 2400
atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 2460
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 2520
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 2580
aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc 2640
tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt 2700
gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa 2760
ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 2820
agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 2880
aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 2940
aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 3000
ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 3060
acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 3120
aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 3180
ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 3240
aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 3300
cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 3360
atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 3420
tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 3480
ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 3540
agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 3600
ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 3660
cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc 3720
attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 3780
cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 3840
tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 3900
tctccattct gagaaagaca cgatcttact agatgaaaat aaaaacaggt gcacgcatcc 3960
tcgcattatc cgcattaacg acgatgatgt tttccgcctc ggctctcgcc aaaatcgaag 4020
aaggtaaact ggtaatctgg attaacggcg ataaaggcta taacggtctc gctgaagtcg 4080
gtaagaaatt cgagaaagat accggaatta aagtcaccgt tgagcatccg gataaactgg 4140
aagagaaatt cccacaggtt gcggcaactg gcgatggccc tgacattatc ttctgggcac 4200
acgaccgctt tggtggctac gctcaatctg gcctgttggc tgaaatcacc ccggacaaag 4260
cgttccagga caagctgtat ccgtttacct gggatgccgt acgttacaac ggcaagctga 4320
ttgcttaccc gatcgctgtt gaagcgttat cgctgattta taacaaagat ctgctgccga 4380
acccgccaaa aacctgggaa gagatcccgg cgctggataa agaactgaaa gcgaaaggta 4440
agagcgcgct gatgttcaac ctgcaagaac cgtacttcac ctggccgctg attgctgctg 4500
acgggggtta tgcgttcaag tatgaaaacg gcaagtacga cattaaagac gtgggcgtgg 4560
ataacgctgg cgcgaaagcg ggtctgacct tcctggttga cctgattaaa aacaaacaca 4620
tgaatgcaga caccgattac tccatcgcag aagctgcctt taataaaggc gaaacagcga 4680
tgaccatcaa cggcccgtgg gcatggtcca acatcgacac cagcaaagtg aattatggtg 4740
taacggtact gccgaccttc aagggtcaac catccaaacc gttcgttggc gtgctgagcg 4800
caggtattaa cgccgccagt ccgaacaaag agctggcaaa agagttcctc gaaaactatc 4860
tgctgactga tgaaggtctg gaagcggtta ataaagacaa accgctgggt gccgtagcgc 4920
tgaagtctta cgaggaagag ttggcgaaag atccacgtat tgccgccacc atggaaaacg 4980
cccagaaagg tgaaatcatg ccgaacatcc cgcagatgtc cgctttctgg tatgccgtgc 5040
gtactgcggt gatcaacgcc gccagcggtc gtcagactgt cgatgaagcc ctgaaagacg 5100
cgcagactaa ttcgagctcg aacaacaaca acaataacaa taacaacaac ctcgggatcg 5160
agggaaggat ttcagaattc gcggaagcgg gtatcaccgg cacgtggtac aaccagcatg 5220
gttctacctt caccgttacc gcgggtgcgg acggtaacct gaccggtcag tacgaaaacc 5280
gtgcgcaggg cactggttgc cagaactctc cgtacaccct gaccggtcgt tacaacggta 5340
ccaaactgga atggcgtgtt gaatggaaca actctaccga aaactgccac tctcgtaccg 5400
aatggcgtgg tcagtaccag ggtggtgcgg aagcgcgtat caacacccag tggaacctga 5460
cctacgaagg tggttctggt ccggcgaccg aacagggtca ggacaccttc accaaagtta 5520
aacaccacca ccaccaccac taagtcgacc tgcaggaagc ttgtttaaac ggtctccagc 5580
ttggctgttt tg 5592
<210> 23
<211> 5594
<212> DNA
<213> Artificial Sequence
<220>
<223> B_R13-p-mSA
<400> 23
gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga 60
taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact 120
cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga 180
actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 240
tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 300
gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat 360
caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg 420
tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 480
gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 540
tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 600
aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 660
cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 720
agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 780
ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 840
tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 900
tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 960
caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1020
accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1080
attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1140
ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1200
taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1260
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1320
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1380
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1440
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1500
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1560
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1620
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1680
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1740
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1800
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1860
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1920
acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1980
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2040
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2100
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2160
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2220
taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2280
cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2340
tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc 2400
atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 2460
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 2520
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 2580
aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc 2640
tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt 2700
gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa 2760
ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 2820
agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 2880
aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 2940
aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 3000
ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 3060
acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 3120
aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 3180
ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 3240
aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 3300
cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 3360
atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 3420
tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 3480
ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 3540
agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 3600
ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 3660
cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc 3720
attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 3780
cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 3840
tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 3900
tctccattct agagaaagag cggatcctac ctagatgaaa ataaaaacag gtgcacgcat 3960
cctcgcatta tccgcattaa cgacgatgat gttttccgcc tcggctctcg ccaaaatcga 4020
agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt 4080
cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact 4140
ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc 4200
acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa 4260
agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct 4320
gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc 4380
gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg 4440
taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc 4500
tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt 4560
ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca 4620
catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc 4680
gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg 4740
tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag 4800
cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta 4860
tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc 4920
gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ccatggaaaa 4980
cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt 5040
gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga 5100
cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat 5160
cgagggaagg atttcagaat tcgcggaagc gggtatcacc ggcacgtggt acaaccagca 5220
tggttctacc ttcaccgtta ccgcgggtgc ggacggtaac ctgaccggtc agtacgaaaa 5280
ccgtgcgcag ggcactggtt gccagaactc tccgtacacc ctgaccggtc gttacaacgg 5340
taccaaactg gaatggcgtg ttgaatggaa caactctacc gaaaactgcc actctcgtac 5400
cgaatggcgt ggtcagtacc agggtggtgc ggaagcgcgt atcaacaccc agtggaacct 5460
gacctacgaa ggtggttctg gtccggcgac cgaacagggt caggacacct tcaccaaagt 5520
taaacaccac caccaccacc actaagtcga cctgcaggaa gcttgtttaa acggtctcca 5580
gcttggctgt tttg 5594
<210> 24
<211> 5593
<212> DNA
<213> Artificial Sequence
<220>
<223> B_R2-p-mSA
<400> 24
gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga 60
taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact 120
cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga 180
actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 240
tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 300
gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat 360
caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg 420
tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 480
gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 540
tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 600
aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 660
cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 720
agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 780
ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 840
tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 900
tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 960
caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1020
accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1080
attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1140
ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1200
taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1260
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1320
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1380
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1440
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1500
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1560
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1620
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1680
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1740
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1800
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1860
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1920
acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1980
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2040
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2100
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2160
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2220
taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2280
cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2340
tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc 2400
atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 2460
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 2520
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 2580
aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc 2640
tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt 2700
gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa 2760
ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 2820
agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 2880
aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 2940
aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 3000
ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 3060
acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 3120
aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 3180
ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 3240
aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 3300
cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 3360
atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 3420
tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 3480
ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 3540
agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 3600
ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 3660
cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc 3720
attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 3780
cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 3840
tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 3900
tctccattct agagaaagat aggagaatac tagatgaaaa taaaaacagg tgcacgcatc 3960
ctcgcattat ccgcattaac gacgatgatg ttttccgcct cggctctcgc caaaatcgaa 4020
gaaggtaaac tggtaatctg gattaacggc gataaaggct ataacggtct cgctgaagtc 4080
ggtaagaaat tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc ggataaactg 4140
gaagagaaat tcccacaggt tgcggcaact ggcgatggcc ctgacattat cttctgggca 4200
cacgaccgct ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa 4260
gcgttccagg acaagctgta tccgtttacc tgggatgccg tacgttacaa cggcaagctg 4320
attgcttacc cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga tctgctgccg 4380
aacccgccaa aaacctggga agagatcccg gcgctggata aagaactgaa agcgaaaggt 4440
aagagcgcgc tgatgttcaa cctgcaagaa ccgtacttca cctggccgct gattgctgct 4500
gacgggggtt atgcgttcaa gtatgaaaac ggcaagtacg acattaaaga cgtgggcgtg 4560
gataacgctg gcgcgaaagc gggtctgacc ttcctggttg acctgattaa aaacaaacac 4620
atgaatgcag acaccgatta ctccatcgca gaagctgcct ttaataaagg cgaaacagcg 4680
atgaccatca acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt gaattatggt 4740
gtaacggtac tgccgacctt caagggtcaa ccatccaaac cgttcgttgg cgtgctgagc 4800
gcaggtatta acgccgccag tccgaacaaa gagctggcaa aagagttcct cgaaaactat 4860
ctgctgactg atgaaggtct ggaagcggtt aataaagaca aaccgctggg tgccgtagcg 4920
ctgaagtctt acgaggaaga gttggcgaaa gatccacgta ttgccgccac catggaaaac 4980
gcccagaaag gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg gtatgccgtg 5040
cgtactgcgg tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc cctgaaagac 5100
gcgcagacta attcgagctc gaacaacaac aacaataaca ataacaacaa cctcgggatc 5160
gagggaagga tttcagaatt cgcggaagcg ggtatcaccg gcacgtggta caaccagcat 5220
ggttctacct tcaccgttac cgcgggtgcg gacggtaacc tgaccggtca gtacgaaaac 5280
cgtgcgcagg gcactggttg ccagaactct ccgtacaccc tgaccggtcg ttacaacggt 5340
accaaactgg aatggcgtgt tgaatggaac aactctaccg aaaactgcca ctctcgtacc 5400
gaatggcgtg gtcagtacca gggtggtgcg gaagcgcgta tcaacaccca gtggaacctg 5460
acctacgaag gtggttctgg tccggcgacc gaacagggtc aggacacctt caccaaagtt 5520
aaacaccacc accaccacca ctaagtcgac ctgcaggaag cttgtttaaa cggtctccag 5580
cttggctgtt ttg 5593
<210> 25
<211> 5593
<212> DNA
<213> Artificial Sequence
<220>
<223> B_R21-p-mSA
<400> 25
gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga 60
taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact 120
cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga 180
actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 240
tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 300
gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat 360
caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg 420
tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 480
gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 540
tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 600
aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 660
cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 720
agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 780
ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 840
tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 900
tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 960
caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1020
accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1080
attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1140
ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1200
taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1260
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1320
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1380
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1440
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1500
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1560
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1620
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1680
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1740
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1800
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1860
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1920
acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1980
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2040
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2100
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2160
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2220
taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2280
cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2340
tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc 2400
atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 2460
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 2520
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 2580
aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc 2640
tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt 2700
gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa 2760
ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 2820
agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 2880
aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 2940
aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 3000
ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 3060
acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 3120
aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 3180
ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 3240
aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 3300
cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 3360
atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 3420
tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 3480
ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 3540
agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 3600
ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 3660
cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc 3720
attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 3780
cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 3840
tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 3900
tctccattct agagaaagag gcgacggtac tagatgaaaa taaaaacagg tgcacgcatc 3960
ctcgcattat ccgcattaac gacgatgatg ttttccgcct cggctctcgc caaaatcgaa 4020
gaaggtaaac tggtaatctg gattaacggc gataaaggct ataacggtct cgctgaagtc 4080
ggtaagaaat tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc ggataaactg 4140
gaagagaaat tcccacaggt tgcggcaact ggcgatggcc ctgacattat cttctgggca 4200
cacgaccgct ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa 4260
gcgttccagg acaagctgta tccgtttacc tgggatgccg tacgttacaa cggcaagctg 4320
attgcttacc cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga tctgctgccg 4380
aacccgccaa aaacctggga agagatcccg gcgctggata aagaactgaa agcgaaaggt 4440
aagagcgcgc tgatgttcaa cctgcaagaa ccgtacttca cctggccgct gattgctgct 4500
gacgggggtt atgcgttcaa gtatgaaaac ggcaagtacg acattaaaga cgtgggcgtg 4560
gataacgctg gcgcgaaagc gggtctgacc ttcctggttg acctgattaa aaacaaacac 4620
atgaatgcag acaccgatta ctccatcgca gaagctgcct ttaataaagg cgaaacagcg 4680
atgaccatca acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt gaattatggt 4740
gtaacggtac tgccgacctt caagggtcaa ccatccaaac cgttcgttgg cgtgctgagc 4800
gcaggtatta acgccgccag tccgaacaaa gagctggcaa aagagttcct cgaaaactat 4860
ctgctgactg atgaaggtct ggaagcggtt aataaagaca aaccgctggg tgccgtagcg 4920
ctgaagtctt acgaggaaga gttggcgaaa gatccacgta ttgccgccac catggaaaac 4980
gcccagaaag gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg gtatgccgtg 5040
cgtactgcgg tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc cctgaaagac 5100
gcgcagacta attcgagctc gaacaacaac aacaataaca ataacaacaa cctcgggatc 5160
gagggaagga tttcagaatt cgcggaagcg ggtatcaccg gcacgtggta caaccagcat 5220
ggttctacct tcaccgttac cgcgggtgcg gacggtaacc tgaccggtca gtacgaaaac 5280
cgtgcgcagg gcactggttg ccagaactct ccgtacaccc tgaccggtcg ttacaacggt 5340
accaaactgg aatggcgtgt tgaatggaac aactctaccg aaaactgcca ctctcgtacc 5400
gaatggcgtg gtcagtacca gggtggtgcg gaagcgcgta tcaacaccca gtggaacctg 5460
acctacgaag gtggttctgg tccggcgacc gaacagggtc aggacacctt caccaaagtt 5520
aaacaccacc accaccacca ctaagtcgac ctgcaggaag cttgtttaaa cggtctccag 5580
cttggctgtt ttg 5593
<210> 26
<211> 13
<212> DNA
<213> Artificial Sequence
<220>
<223> BBa_B0032
<400> 26
tcacacagga aag 13
<210> 27
<211> 15
<212> DNA
<213> Artificial Sequence
<220>
<223> BBa_B0030
<400> 27
attaaagagg agaaa 15
<210> 28
<211> 12
<212> DNA
<213> Artificial Sequence
<220>
<223> BBa_B0034
<400> 28
aaagaggaga aa 12
<210> 29
<211> 39
<212> DNA
<213> Artificial Sequence
<220>
<223> regulatory gene
<400> 29
acccgttttt tgggctaaca ggaggaagct agcgctagc 39
<210> 30
<211> 27
<212> DNA
<213> Artificial Sequence
<220>
<223> regulatory gene
<400> 30
tagcactcgt tgacatacgg acgtcac 27
<210> 31
<211> 15
<212> DNA
<213> Artificial Sequence
<220>
<223> regulatory gene
<400> 31
actactgagg ctact 15
<210> 32
<211> 30
<212> DNA
<213> Artificial Sequence
<220>
<223> regulatory gene
<400> 32
tggaacagct cacgcaaaaa taggtttctt 30
<210> 33
<211> 34
<212> DNA
<213> Artificial Sequence
<220>
<223> regulatory gene
<400> 33
cgctttttat cgcaactctc tactgtttct ccat 34
<210> 34
<211> 25
<212> DNA
<213> Artificial Sequence
<220>
<223> regulatory gene
<400> 34
tctgagaaag acacgatctt actag 25
<210> 35
<211> 27
<212> DNA
<213> Artificial Sequence
<220>
<223> regulatory gene
<400> 35
tctagagaaa gagcggatcc tacctag 27
<210> 36
<211> 26
<212> DNA
<213> Artificial Sequence
<220>
<223> regulatory gene
<400> 36
tctagagaaa gataggagaa tactag 26
<210> 37
<211> 26
<212> DNA
<213> Artificial Sequence
<220>
<223> regulatory gene
<400> 37
tctagagaaa gaggcgacgg tactag 26
<210> 38
<211> 6
<212> PRT
<213> Artificial Sequence
<220>
<223> HJ inhibitor peptide 2
<400> 38
Lys Trp Trp Cys Arg Trp
1 5
<210> 39
<211> 5593
<212> DNA
<213> Artificial Sequence
<220>
<223> pBAD_R-lib-1-1-mSA
<400> 39
gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga 60
taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact 120
cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga 180
actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 240
tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 300
gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat 360
caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg 420
tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 480
gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 540
tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 600
aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 660
cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 720
agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 780
ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 840
tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 900
tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 960
caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1020
accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1080
attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1140
ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1200
taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1260
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1320
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1380
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1440
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1500
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1560
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1620
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1680
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1740
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1800
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1860
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1920
acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1980
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2040
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2100
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2160
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2220
taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2280
cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2340
tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc 2400
atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 2460
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 2520
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 2580
aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc 2640
tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt 2700
gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa 2760
ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 2820
agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 2880
aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 2940
aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 3000
ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 3060
acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 3120
aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 3180
ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 3240
aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 3300
cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 3360
atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 3420
tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 3480
ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 3540
agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 3600
ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 3660
cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc 3720
attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 3780
cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 3840
tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 3900
tctccattct agagaaagag gcgagtgtac tagatgaaaa taaaaacagg tgcacgcatc 3960
ctcgcattat ccgcattaac gacgatgatg ttttccgcct cggctctcgc caaaatcgaa 4020
gaaggtaaac tggtaatctg gattaacggc gataaaggct ataacggtct cgctgaagtc 4080
ggtaagaaat tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc ggataaactg 4140
gaagagaaat tcccacaggt tgcggcaact ggcgatggcc ctgacattat cttctgggca 4200
cacgaccgct ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa 4260
gcgttccagg acaagctgta tccgtttacc tgggatgccg tacgttacaa cggcaagctg 4320
attgcttacc cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga tctgctgccg 4380
aacccgccaa aaacctggga agagatcccg gcgctggata aagaactgaa agcgaaaggt 4440
aagagcgcgc tgatgttcaa cctgcaagaa ccgtacttca cctggccgct gattgctgct 4500
gacgggggtt atgcgttcaa gtatgaaaac ggcaagtacg acattaaaga cgtgggcgtg 4560
gataacgctg gcgcgaaagc gggtctgacc ttcctggttg acctgattaa aaacaaacac 4620
atgaatgcag acaccgatta ctccatcgca gaagctgcct ttaataaagg cgaaacagcg 4680
atgaccatca acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt gaattatggt 4740
gtaacggtac tgccgacctt caagggtcaa ccatccaaac cgttcgttgg cgtgctgagc 4800
gcaggtatta acgccgccag tccgaacaaa gagctggcaa aagagttcct cgaaaactat 4860
ctgctgactg atgaaggtct ggaagcggtt aataaagaca aaccgctggg tgccgtagcg 4920
ctgaagtctt acgaggaaga gttggcgaaa gatccacgta ttgccgccac catggaaaac 4980
gcccagaaag gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg gtatgccgtg 5040
cgtactgcgg tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc cctgaaagac 5100
gcgcagacta attcgagctc gaacaacaac aacaataaca ataacaacaa cctcgggatc 5160
gagggaagga tttcagaatt cgcggaagcg ggtatcaccg gcacgtggta caaccagcat 5220
ggttctacct tcaccgttac cgcgggtgcg gacggtaacc tgaccggtca gtacgaaaac 5280
cgtgcgcagg gcactggttg ccagaactct ccgtacaccc tgaccggtcg ttacaacggt 5340
accaaactgg aatggcgtgt tgaatggaac aactctaccg aaaactgcca ctctcgtacc 5400
gaatggcgtg gtcagtacca gggtggtgcg gaagcgcgta tcaacaccca gtggaacctg 5460
acctacgaag gtggttctgg tccggcgacc gaacagggtc aggacacctt caccaaagtt 5520
aaacaccacc accaccacca ctaagtcgac ctgcaggaag cttgtttaaa cggtctccag 5580
cttggctgtt ttg 5593
<210> 40
<211> 5593
<212> DNA
<213> Artificial Sequence
<220>
<223> pBAD_R-lib-1-5-mSA
<400> 40
gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga 60
taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact 120
cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga 180
actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 240
tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 300
gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat 360
caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg 420
tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 480
gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 540
tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 600
aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 660
cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 720
agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 780
ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 840
tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 900
tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 960
caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1020
accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1080
attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1140
ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1200
taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1260
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1320
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1380
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1440
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1500
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1560
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1620
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1680
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1740
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1800
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1860
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1920
acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1980
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2040
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2100
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2160
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2220
taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2280
cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2340
tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc 2400
atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 2460
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 2520
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 2580
aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc 2640
tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt 2700
gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa 2760
ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 2820
agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 2880
aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 2940
aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 3000
ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 3060
acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 3120
aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 3180
ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 3240
aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 3300
cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 3360
atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 3420
tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 3480
ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 3540
agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 3600
ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 3660
cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc 3720
attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 3780
cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 3840
tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 3900
tctccattct agagaaagat aggaggttac tagatgaaaa taaaaacagg tgcacgcatc 3960
ctcgcattat ccgcattaac gacgatgatg ttttccgcct cggctctcgc caaaatcgaa 4020
gaaggtaaac tggtaatctg gattaacggc gataaaggct ataacggtct cgctgaagtc 4080
ggtaagaaat tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc ggataaactg 4140
gaagagaaat tcccacaggt tgcggcaact ggcgatggcc ctgacattat cttctgggca 4200
cacgaccgct ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa 4260
gcgttccagg acaagctgta tccgtttacc tgggatgccg tacgttacaa cggcaagctg 4320
attgcttacc cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga tctgctgccg 4380
aacccgccaa aaacctggga agagatcccg gcgctggata aagaactgaa agcgaaaggt 4440
aagagcgcgc tgatgttcaa cctgcaagaa ccgtacttca cctggccgct gattgctgct 4500
gacgggggtt atgcgttcaa gtatgaaaac ggcaagtacg acattaaaga cgtgggcgtg 4560
gataacgctg gcgcgaaagc gggtctgacc ttcctggttg acctgattaa aaacaaacac 4620
atgaatgcag acaccgatta ctccatcgca gaagctgcct ttaataaagg cgaaacagcg 4680
atgaccatca acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt gaattatggt 4740
gtaacggtac tgccgacctt caagggtcaa ccatccaaac cgttcgttgg cgtgctgagc 4800
gcaggtatta acgccgccag tccgaacaaa gagctggcaa aagagttcct cgaaaactat 4860
ctgctgactg atgaaggtct ggaagcggtt aataaagaca aaccgctggg tgccgtagcg 4920
ctgaagtctt acgaggaaga gttggcgaaa gatccacgta ttgccgccac catggaaaac 4980
gcccagaaag gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg gtatgccgtg 5040
cgtactgcgg tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc cctgaaagac 5100
gcgcagacta attcgagctc gaacaacaac aacaataaca ataacaacaa cctcgggatc 5160
gagggaagga tttcagaatt cgcggaagcg ggtatcaccg gcacgtggta caaccagcat 5220
ggttctacct tcaccgttac cgcgggtgcg gacggtaacc tgaccggtca gtacgaaaac 5280
cgtgcgcagg gcactggttg ccagaactct ccgtacaccc tgaccggtcg ttacaacggt 5340
accaaactgg aatggcgtgt tgaatggaac aactctaccg aaaactgcca ctctcgtacc 5400
gaatggcgtg gtcagtacca gggtggtgcg gaagcgcgta tcaacaccca gtggaacctg 5460
acctacgaag gtggttctgg tccggcgacc gaacagggtc aggacacctt caccaaagtt 5520
aaacaccacc accaccacca ctaagtcgac ctgcaggaag cttgtttaaa cggtctccag 5580
cttggctgtt ttg 5593
<210> 41
<211> 5593
<212> DNA
<213> Artificial Sequence
<220>
<223> pBAD_R-lib-1-7-mSA
<400> 41
gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga 60
taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact 120
cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga 180
actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 240
tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 300
gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat 360
caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg 420
tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 480
gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 540
tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 600
aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 660
cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 720
agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 780
ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 840
tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 900
tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 960
caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1020
accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1080
attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1140
ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1200
taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1260
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1320
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1380
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1440
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1500
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1560
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1620
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1680
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1740
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1800
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1860
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1920
acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1980
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2040
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2100
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2160
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2220
taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2280
cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2340
tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc 2400
atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 2460
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 2520
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 2580
aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc 2640
tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt 2700
gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa 2760
ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 2820
agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 2880
aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 2940
aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 3000
ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 3060
acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 3120
aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 3180
ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 3240
aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 3300
cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 3360
atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 3420
tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 3480
ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 3540
agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 3600
ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 3660
cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc 3720
attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 3780
cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 3840
tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 3900
tctccattct agagaaagag gggacactac tagatgaaaa taaaaacagg tgcacgcatc 3960
ctcgcattat ccgcattaac gacgatgatg ttttccgcct cggctctcgc caaaatcgaa 4020
gaaggtaaac tggtaatctg gattaacggc gataaaggct ataacggtct cgctgaagtc 4080
ggtaagaaat tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc ggataaactg 4140
gaagagaaat tcccacaggt tgcggcaact ggcgatggcc ctgacattat cttctgggca 4200
cacgaccgct ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa 4260
gcgttccagg acaagctgta tccgtttacc tgggatgccg tacgttacaa cggcaagctg 4320
attgcttacc cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga tctgctgccg 4380
aacccgccaa aaacctggga agagatcccg gcgctggata aagaactgaa agcgaaaggt 4440
aagagcgcgc tgatgttcaa cctgcaagaa ccgtacttca cctggccgct gattgctgct 4500
gacgggggtt atgcgttcaa gtatgaaaac ggcaagtacg acattaaaga cgtgggcgtg 4560
gataacgctg gcgcgaaagc gggtctgacc ttcctggttg acctgattaa aaacaaacac 4620
atgaatgcag acaccgatta ctccatcgca gaagctgcct ttaataaagg cgaaacagcg 4680
atgaccatca acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt gaattatggt 4740
gtaacggtac tgccgacctt caagggtcaa ccatccaaac cgttcgttgg cgtgctgagc 4800
gcaggtatta acgccgccag tccgaacaaa gagctggcaa aagagttcct cgaaaactat 4860
ctgctgactg atgaaggtct ggaagcggtt aataaagaca aaccgctggg tgccgtagcg 4920
ctgaagtctt acgaggaaga gttggcgaaa gatccacgta ttgccgccac catggaaaac 4980
gcccagaaag gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg gtatgccgtg 5040
cgtactgcgg tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc cctgaaagac 5100
gcgcagacta attcgagctc gaacaacaac aacaataaca ataacaacaa cctcgggatc 5160
gagggaagga tttcagaatt cgcggaagcg ggtatcaccg gcacgtggta caaccagcat 5220
ggttctacct tcaccgttac cgcgggtgcg gacggtaacc tgaccggtca gtacgaaaac 5280
cgtgcgcagg gcactggttg ccagaactct ccgtacaccc tgaccggtcg ttacaacggt 5340
accaaactgg aatggcgtgt tgaatggaac aactctaccg aaaactgcca ctctcgtacc 5400
gaatggcgtg gtcagtacca gggtggtgcg gaagcgcgta tcaacaccca gtggaacctg 5460
acctacgaag gtggttctgg tccggcgacc gaacagggtc aggacacctt caccaaagtt 5520
aaacaccacc accaccacca ctaagtcgac ctgcaggaag cttgtttaaa cggtctccag 5580
cttggctgtt ttg 5593
<210> 42
<211> 5593
<212> DNA
<213> Artificial Sequence
<220>
<223> pBAD_R-lib-1-10-mSA
<400> 42
gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga 60
taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact 120
cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga 180
actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 240
tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 300
gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat 360
caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg 420
tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 480
gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 540
tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 600
aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 660
cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 720
agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 780
ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 840
tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 900
tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 960
caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1020
accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1080
attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1140
ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1200
taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1260
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1320
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1380
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1440
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1500
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1560
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1620
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1680
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1740
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1800
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1860
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1920
acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1980
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2040
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2100
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2160
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2220
taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2280
cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2340
tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc 2400
atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 2460
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 2520
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 2580
aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc 2640
tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt 2700
gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa 2760
ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 2820
agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 2880
aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 2940
aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 3000
ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 3060
acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 3120
aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 3180
ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 3240
aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 3300
cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 3360
atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 3420
tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 3480
ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 3540
agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 3600
ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 3660
cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc 3720
attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 3780
cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 3840
tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 3900
tctccattct agagaaagag cggaaactac tagatgaaaa taaaaacagg tgcacgcatc 3960
ctcgcattat ccgcattaac gacgatgatg ttttccgcct cggctctcgc caaaatcgaa 4020
gaaggtaaac tggtaatctg gattaacggc gataaaggct ataacggtct cgctgaagtc 4080
ggtaagaaat tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc ggataaactg 4140
gaagagaaat tcccacaggt tgcggcaact ggcgatggcc ctgacattat cttctgggca 4200
cacgaccgct ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa 4260
gcgttccagg acaagctgta tccgtttacc tgggatgccg tacgttacaa cggcaagctg 4320
attgcttacc cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga tctgctgccg 4380
aacccgccaa aaacctggga agagatcccg gcgctggata aagaactgaa agcgaaaggt 4440
aagagcgcgc tgatgttcaa cctgcaagaa ccgtacttca cctggccgct gattgctgct 4500
gacgggggtt atgcgttcaa gtatgaaaac ggcaagtacg acattaaaga cgtgggcgtg 4560
gataacgctg gcgcgaaagc gggtctgacc ttcctggttg acctgattaa aaacaaacac 4620
atgaatgcag acaccgatta ctccatcgca gaagctgcct ttaataaagg cgaaacagcg 4680
atgaccatca acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt gaattatggt 4740
gtaacggtac tgccgacctt caagggtcaa ccatccaaac cgttcgttgg cgtgctgagc 4800
gcaggtatta acgccgccag tccgaacaaa gagctggcaa aagagttcct cgaaaactat 4860
ctgctgactg atgaaggtct ggaagcggtt aataaagaca aaccgctggg tgccgtagcg 4920
ctgaagtctt acgaggaaga gttggcgaaa gatccacgta ttgccgccac catggaaaac 4980
gcccagaaag gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg gtatgccgtg 5040
cgtactgcgg tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc cctgaaagac 5100
gcgcagacta attcgagctc gaacaacaac aacaataaca ataacaacaa cctcgggatc 5160
gagggaagga tttcagaatt cgcggaagcg ggtatcaccg gcacgtggta caaccagcat 5220
ggttctacct tcaccgttac cgcgggtgcg gacggtaacc tgaccggtca gtacgaaaac 5280
cgtgcgcagg gcactggttg ccagaactct ccgtacaccc tgaccggtcg ttacaacggt 5340
accaaactgg aatggcgtgt tgaatggaac aactctaccg aaaactgcca ctctcgtacc 5400
gaatggcgtg gtcagtacca gggtggtgcg gaagcgcgta tcaacaccca gtggaacctg 5460
acctacgaag gtggttctgg tccggcgacc gaacagggtc aggacacctt caccaaagtt 5520
aaacaccacc accaccacca ctaagtcgac ctgcaggaag cttgtttaaa cggtctccag 5580
cttggctgtt ttg 5593
<210> 43
<211> 5594
<212> DNA
<213> Artificial Sequence
<220>
<223> pBAD_R-lib-1-11-mSA
<400> 43
gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga 60
taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact 120
cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga 180
actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 240
tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 300
gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat 360
caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg 420
tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 480
gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 540
tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 600
aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 660
cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 720
agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 780
ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 840
tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 900
tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 960
caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1020
accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1080
attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1140
ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1200
taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1260
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1320
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1380
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1440
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1500
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1560
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1620
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1680
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1740
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1800
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1860
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1920
acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1980
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2040
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2100
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2160
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2220
taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2280
cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2340
tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc 2400
atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 2460
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 2520
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 2580
aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc 2640
tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt 2700
gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa 2760
ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 2820
agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 2880
aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 2940
aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 3000
ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 3060
acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 3120
aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 3180
ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 3240
aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 3300
cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 3360
atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 3420
tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 3480
ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 3540
agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 3600
ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 3660
cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc 3720
attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 3780
cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 3840
tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 3900
tctccatttc tagagaaaga tttgaatata ctagatgaaa ataaaaacag gtgcacgcat 3960
cctcgcatta tccgcattaa cgacgatgat gttttccgcc tcggctctcg ccaaaatcga 4020
agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt 4080
cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact 4140
ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc 4200
acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa 4260
agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct 4320
gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc 4380
gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg 4440
taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc 4500
tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt 4560
ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca 4620
catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc 4680
gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg 4740
tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag 4800
cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta 4860
tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc 4920
gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ccatggaaaa 4980
cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt 5040
gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga 5100
cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat 5160
cgagggaagg atttcagaat tcgcggaagc gggtatcacc ggcacgtggt acaaccagca 5220
tggttctacc ttcaccgtta ccgcgggtgc ggacggtaac ctgaccggtc agtacgaaaa 5280
ccgtgcgcag ggcactggtt gccagaactc tccgtacacc ctgaccggtc gttacaacgg 5340
taccaaactg gaatggcgtg ttgaatggaa caactctacc gaaaactgcc actctcgtac 5400
cgaatggcgt ggtcagtacc agggtggtgc ggaagcgcgt atcaacaccc agtggaacct 5460
gacctacgaa ggtggttctg gtccggcgac cgaacagggt caggacacct tcaccaaagt 5520
taaacaccac caccaccacc actaagtcga cctgcaggaa gcttgtttaa acggtctcca 5580
gcttggctgt tttg 5594
<210> 44
<211> 5593
<212> DNA
<213> Artificial Sequence
<220>
<223> pBAD_R-lib-1-12-mSA
<400> 44
gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga 60
taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact 120
cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga 180
actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 240
tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 300
gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat 360
caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg 420
tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 480
gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 540
tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 600
aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 660
cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 720
agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 780
ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 840
tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 900
tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 960
caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1020
accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1080
attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1140
ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1200
taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1260
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1320
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1380
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1440
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1500
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1560
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1620
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1680
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1740
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1800
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1860
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1920
acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1980
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2040
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2100
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2160
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2220
taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2280
cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2340
tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc 2400
atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 2460
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 2520
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 2580
aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc 2640
tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt 2700
gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa 2760
ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 2820
agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 2880
aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 2940
aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 3000
ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 3060
acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 3120
aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 3180
ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 3240
aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 3300
cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 3360
atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 3420
tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 3480
ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 3540
agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 3600
ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 3660
cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc 3720
attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 3780
cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 3840
tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 3900
tctccattct agagaaagaa cggacattac tagatgaaaa taaaaacagg tgcacgcatc 3960
ctcgcattat ccgcattaac gacgatgatg ttttccgcct cggctctcgc caaaatcgaa 4020
gaaggtaaac tggtaatctg gattaacggc gataaaggct ataacggtct cgctgaagtc 4080
ggtaagaaat tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc ggataaactg 4140
gaagagaaat tcccacaggt tgcggcaact ggcgatggcc ctgacattat cttctgggca 4200
cacgaccgct ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa 4260
gcgttccagg acaagctgta tccgtttacc tgggatgccg tacgttacaa cggcaagctg 4320
attgcttacc cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga tctgctgccg 4380
aacccgccaa aaacctggga agagatcccg gcgctggata aagaactgaa agcgaaaggt 4440
aagagcgcgc tgatgttcaa cctgcaagaa ccgtacttca cctggccgct gattgctgct 4500
gacgggggtt atgcgttcaa gtatgaaaac ggcaagtacg acattaaaga cgtgggcgtg 4560
gataacgctg gcgcgaaagc gggtctgacc ttcctggttg acctgattaa aaacaaacac 4620
atgaatgcag acaccgatta ctccatcgca gaagctgcct ttaataaagg cgaaacagcg 4680
atgaccatca acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt gaattatggt 4740
gtaacggtac tgccgacctt caagggtcaa ccatccaaac cgttcgttgg cgtgctgagc 4800
gcaggtatta acgccgccag tccgaacaaa gagctggcaa aagagttcct cgaaaactat 4860
ctgctgactg atgaaggtct ggaagcggtt aataaagaca aaccgctggg tgccgtagcg 4920
ctgaagtctt acgaggaaga gttggcgaaa gatccacgta ttgccgccac catggaaaac 4980
gcccagaaag gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg gtatgccgtg 5040
cgtactgcgg tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc cctgaaagac 5100
gcgcagacta attcgagctc gaacaacaac aacaataaca ataacaacaa cctcgggatc 5160
gagggaagga tttcagaatt cgcggaagcg ggtatcaccg gcacgtggta caaccagcat 5220
ggttctacct tcaccgttac cgcgggtgcg gacggtaacc tgaccggtca gtacgaaaac 5280
cgtgcgcagg gcactggttg ccagaactct ccgtacaccc tgaccggtcg ttacaacggt 5340
accaaactgg aatggcgtgt tgaatggaac aactctaccg aaaactgcca ctctcgtacc 5400
gaatggcgtg gtcagtacca gggtggtgcg gaagcgcgta tcaacaccca gtggaacctg 5460
acctacgaag gtggttctgg tccggcgacc gaacagggtc aggacacctt caccaaagtt 5520
aaacaccacc accaccacca ctaagtcgac ctgcaggaag cttgtttaaa cggtctccag 5580
cttggctgtt ttg 5593
<210> 45
<211> 5593
<212> DNA
<213> Artificial Sequence
<220>
<223> pBAD_R-lib-1-13-mSA
<400> 45
gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga 60
taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact 120
cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga 180
actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 240
tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 300
gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat 360
caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg 420
tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 480
gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 540
tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 600
aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 660
cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 720
agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 780
ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 840
tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 900
tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 960
caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1020
accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1080
attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1140
ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1200
taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1260
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1320
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1380
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1440
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1500
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1560
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1620
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1680
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1740
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1800
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1860
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1920
acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1980
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2040
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2100
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2160
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2220
taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2280
cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2340
tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc 2400
atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 2460
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 2520
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 2580
aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc 2640
tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt 2700
gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa 2760
ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 2820
agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 2880
aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 2940
aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 3000
ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 3060
acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 3120
aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 3180
ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 3240
aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 3300
cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 3360
atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 3420
tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 3480
ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 3540
agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 3600
ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 3660
cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc 3720
attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 3780
cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 3840
tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 3900
tctccattct agagaaagac atgactatac tagatgaaaa taaaaacagg tgcacgcatc 3960
ctcgcattat ccgcattaac gacgatgatg ttttccgcct cggctctcgc caaaatcgaa 4020
gaaggtaaac tggtaatctg gattaacggc gataaaggct ataacggtct cgctgaagtc 4080
ggtaagaaat tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc ggataaactg 4140
gaagagaaat tcccacaggt tgcggcaact ggcgatggcc ctgacattat cttctgggca 4200
cacgaccgct ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa 4260
gcgttccagg acaagctgta tccgtttacc tgggatgccg tacgttacaa cggcaagctg 4320
attgcttacc cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga tctgctgccg 4380
aacccgccaa aaacctggga agagatcccg gcgctggata aagaactgaa agcgaaaggt 4440
aagagcgcgc tgatgttcaa cctgcaagaa ccgtacttca cctggccgct gattgctgct 4500
gacgggggtt atgcgttcaa gtatgaaaac ggcaagtacg acattaaaga cgtgggcgtg 4560
gataacgctg gcgcgaaagc gggtctgacc ttcctggttg acctgattaa aaacaaacac 4620
atgaatgcag acaccgatta ctccatcgca gaagctgcct ttaataaagg cgaaacagcg 4680
atgaccatca acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt gaattatggt 4740
gtaacggtac tgccgacctt caagggtcaa ccatccaaac cgttcgttgg cgtgctgagc 4800
gcaggtatta acgccgccag tccgaacaaa gagctggcaa aagagttcct cgaaaactat 4860
ctgctgactg atgaaggtct ggaagcggtt aataaagaca aaccgctggg tgccgtagcg 4920
ctgaagtctt acgaggaaga gttggcgaaa gatccacgta ttgccgccac catggaaaac 4980
gcccagaaag gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg gtatgccgtg 5040
cgtactgcgg tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc cctgaaagac 5100
gcgcagacta attcgagctc gaacaacaac aacaataaca ataacaacaa cctcgggatc 5160
gagggaagga tttcagaatt cgcggaagcg ggtatcaccg gcacgtggta caaccagcat 5220
ggttctacct tcaccgttac cgcgggtgcg gacggtaacc tgaccggtca gtacgaaaac 5280
cgtgcgcagg gcactggttg ccagaactct ccgtacaccc tgaccggtcg ttacaacggt 5340
accaaactgg aatggcgtgt tgaatggaac aactctaccg aaaactgcca ctctcgtacc 5400
gaatggcgtg gtcagtacca gggtggtgcg gaagcgcgta tcaacaccca gtggaacctg 5460
acctacgaag gtggttctgg tccggcgacc gaacagggtc aggacacctt caccaaagtt 5520
aaacaccacc accaccacca ctaagtcgac ctgcaggaag cttgtttaaa cggtctccag 5580
cttggctgtt ttg 5593
<210> 46
<211> 5593
<212> DNA
<213> Artificial Sequence
<220>
<223> pBAD_R-lib-1-14-mSA
<400> 46
gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga 60
taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact 120
cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga 180
actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 240
tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 300
gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat 360
caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg 420
tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 480
gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 540
tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 600
aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 660
cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 720
agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 780
ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 840
tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 900
tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 960
caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1020
accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1080
attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1140
ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1200
taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1260
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1320
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1380
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1440
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1500
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1560
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1620
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1680
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1740
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1800
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1860
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1920
acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1980
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2040
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2100
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2160
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2220
taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2280
cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2340
tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc 2400
atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 2460
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 2520
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 2580
aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc 2640
tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt 2700
gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa 2760
ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 2820
agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 2880
aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 2940
aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 3000
ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 3060
acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 3120
aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 3180
ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 3240
aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 3300
cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 3360
atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 3420
tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 3480
ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 3540
agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 3600
ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 3660
cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc 3720
attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 3780
cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 3840
tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 3900
tctccattct agagaaagaa ctgaagatac tagatgaaaa taaaaacagg tgcacgcatc 3960
ctcgcattat ccgcattaac gacgatgatg ttttccgcct cggctctcgc caaaatcgaa 4020
gaaggtaaac tggtaatctg gattaacggc gataaaggct ataacggtct cgctgaagtc 4080
ggtaagaaat tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc ggataaactg 4140
gaagagaaat tcccacaggt tgcggcaact ggcgatggcc ctgacattat cttctgggca 4200
cacgaccgct ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa 4260
gcgttccagg acaagctgta tccgtttacc tgggatgccg tacgttacaa cggcaagctg 4320
attgcttacc cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga tctgctgccg 4380
aacccgccaa aaacctggga agagatcccg gcgctggata aagaactgaa agcgaaaggt 4440
aagagcgcgc tgatgttcaa cctgcaagaa ccgtacttca cctggccgct gattgctgct 4500
gacgggggtt atgcgttcaa gtatgaaaac ggcaagtacg acattaaaga cgtgggcgtg 4560
gataacgctg gcgcgaaagc gggtctgacc ttcctggttg acctgattaa aaacaaacac 4620
atgaatgcag acaccgatta ctccatcgca gaagctgcct ttaataaagg cgaaacagcg 4680
atgaccatca acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt gaattatggt 4740
gtaacggtac tgccgacctt caagggtcaa ccatccaaac cgttcgttgg cgtgctgagc 4800
gcaggtatta acgccgccag tccgaacaaa gagctggcaa aagagttcct cgaaaactat 4860
ctgctgactg atgaaggtct ggaagcggtt aataaagaca aaccgctggg tgccgtagcg 4920
ctgaagtctt acgaggaaga gttggcgaaa gatccacgta ttgccgccac catggaaaac 4980
gcccagaaag gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg gtatgccgtg 5040
cgtactgcgg tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc cctgaaagac 5100
gcgcagacta attcgagctc gaacaacaac aacaataaca ataacaacaa cctcgggatc 5160
gagggaagga tttcagaatt cgcggaagcg ggtatcaccg gcacgtggta caaccagcat 5220
ggttctacct tcaccgttac cgcgggtgcg gacggtaacc tgaccggtca gtacgaaaac 5280
cgtgcgcagg gcactggttg ccagaactct ccgtacaccc tgaccggtcg ttacaacggt 5340
accaaactgg aatggcgtgt tgaatggaac aactctaccg aaaactgcca ctctcgtacc 5400
gaatggcgtg gtcagtacca gggtggtgcg gaagcgcgta tcaacaccca gtggaacctg 5460
acctacgaag gtggttctgg tccggcgacc gaacagggtc aggacacctt caccaaagtt 5520
aaacaccacc accaccacca ctaagtcgac ctgcaggaag cttgtttaaa cggtctccag 5580
cttggctgtt ttg 5593
<210> 47
<211> 5593
<212> DNA
<213> Artificial Sequence
<220>
<223> pBAD_R-lib-1-16-mSA
<400> 47
gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga 60
taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact 120
cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga 180
actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 240
tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 300
gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat 360
caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg 420
tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 480
gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 540
tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 600
aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 660
cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 720
agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 780
ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 840
tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 900
tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 960
caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1020
accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1080
attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1140
ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1200
taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1260
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1320
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1380
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1440
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1500
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1560
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1620
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1680
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1740
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1800
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1860
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1920
acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1980
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2040
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2100
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2160
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2220
taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2280
cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2340
tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc 2400
atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 2460
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 2520
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 2580
aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc 2640
tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt 2700
gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa 2760
ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 2820
agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 2880
aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 2940
aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 3000
ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 3060
acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 3120
aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 3180
ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 3240
aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 3300
cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 3360
atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 3420
tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 3480
ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 3540
agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 3600
ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 3660
cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc 3720
attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 3780
cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 3840
tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 3900
tctccattct agagaaagag gcgatcctac tagatgaaaa taaaaacagg tgcacgcatc 3960
ctcgcattat ccgcattaac gacgatgatg ttttccgcct cggctctcgc caaaatcgaa 4020
gaaggtaaac tggtaatctg gattaacggc gataaaggct ataacggtct cgctgaagtc 4080
ggtaagaaat tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc ggataaactg 4140
gaagagaaat tcccacaggt tgcggcaact ggcgatggcc ctgacattat cttctgggca 4200
cacgaccgct ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa 4260
gcgttccagg acaagctgta tccgtttacc tgggatgccg tacgttacaa cggcaagctg 4320
attgcttacc cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga tctgctgccg 4380
aacccgccaa aaacctggga agagatcccg gcgctggata aagaactgaa agcgaaaggt 4440
aagagcgcgc tgatgttcaa cctgcaagaa ccgtacttca cctggccgct gattgctgct 4500
gacgggggtt atgcgttcaa gtatgaaaac ggcaagtacg acattaaaga cgtgggcgtg 4560
gataacgctg gcgcgaaagc gggtctgacc ttcctggttg acctgattaa aaacaaacac 4620
atgaatgcag acaccgatta ctccatcgca gaagctgcct ttaataaagg cgaaacagcg 4680
atgaccatca acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt gaattatggt 4740
gtaacggtac tgccgacctt caagggtcaa ccatccaaac cgttcgttgg cgtgctgagc 4800
gcaggtatta acgccgccag tccgaacaaa gagctggcaa aagagttcct cgaaaactat 4860
ctgctgactg atgaaggtct ggaagcggtt aataaagaca aaccgctggg tgccgtagcg 4920
ctgaagtctt acgaggaaga gttggcgaaa gatccacgta ttgccgccac catggaaaac 4980
gcccagaaag gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg gtatgccgtg 5040
cgtactgcgg tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc cctgaaagac 5100
gcgcagacta attcgagctc gaacaacaac aacaataaca ataacaacaa cctcgggatc 5160
gagggaagga tttcagaatt cgcggaagcg ggtatcaccg gcacgtggta caaccagcat 5220
ggttctacct tcaccgttac cgcgggtgcg gacggtaacc tgaccggtca gtacgaaaac 5280
cgtgcgcagg gcactggttg ccagaactct ccgtacaccc tgaccggtcg ttacaacggt 5340
accaaactgg aatggcgtgt tgaatggaac aactctaccg aaaactgcca ctctcgtacc 5400
gaatggcgtg gtcagtacca gggtggtgcg gaagcgcgta tcaacaccca gtggaacctg 5460
acctacgaag gtggttctgg tccggcgacc gaacagggtc aggacacctt caccaaagtt 5520
aaacaccacc accaccacca ctaagtcgac ctgcaggaag cttgtttaaa cggtctccag 5580
cttggctgtt ttg 5593
<210> 48
<211> 5593
<212> DNA
<213> Artificial Sequence
<220>
<223> pBAD_R-lib-1-17-mSA
<400> 48
gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga 60
taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact 120
cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga 180
actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 240
tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 300
gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat 360
caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg 420
tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 480
gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 540
tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 600
aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 660
cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 720
agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 780
ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 840
tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 900
tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 960
caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1020
accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1080
attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1140
ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1200
taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1260
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1320
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1380
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1440
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1500
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1560
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1620
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1680
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1740
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1800
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1860
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1920
acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1980
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2040
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2100
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2160
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2220
taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2280
cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2340
tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc 2400
atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 2460
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 2520
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 2580
aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc 2640
tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt 2700
gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa 2760
ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 2820
agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 2880
aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 2940
aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 3000
ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 3060
acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 3120
aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 3180
ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 3240
aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 3300
cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 3360
atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 3420
tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 3480
ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 3540
agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 3600
ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 3660
cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc 3720
attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 3780
cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 3840
tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 3900
tctccattct agagaaagaa gagagcctac tagatgaaaa taaaaacagg tgcacgcatc 3960
ctcgcattat ccgcattaac gacgatgatg ttttccgcct cggctctcgc caaaatcgaa 4020
gaaggtaaac tggtaatctg gattaacggc gataaaggct ataacggtct cgctgaagtc 4080
ggtaagaaat tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc ggataaactg 4140
gaagagaaat tcccacaggt tgcggcaact ggcgatggcc ctgacattat cttctgggca 4200
cacgaccgct ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa 4260
gcgttccagg acaagctgta tccgtttacc tgggatgccg tacgttacaa cggcaagctg 4320
attgcttacc cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga tctgctgccg 4380
aacccgccaa aaacctggga agagatcccg gcgctggata aagaactgaa agcgaaaggt 4440
aagagcgcgc tgatgttcaa cctgcaagaa ccgtacttca cctggccgct gattgctgct 4500
gacgggggtt atgcgttcaa gtatgaaaac ggcaagtacg acattaaaga cgtgggcgtg 4560
gataacgctg gcgcgaaagc gggtctgacc ttcctggttg acctgattaa aaacaaacac 4620
atgaatgcag acaccgatta ctccatcgca gaagctgcct ttaataaagg cgaaacagcg 4680
atgaccatca acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt gaattatggt 4740
gtaacggtac tgccgacctt caagggtcaa ccatccaaac cgttcgttgg cgtgctgagc 4800
gcaggtatta acgccgccag tccgaacaaa gagctggcaa aagagttcct cgaaaactat 4860
ctgctgactg atgaaggtct ggaagcggtt aataaagaca aaccgctggg tgccgtagcg 4920
ctgaagtctt acgaggaaga gttggcgaaa gatccacgta ttgccgccac catggaaaac 4980
gcccagaaag gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg gtatgccgtg 5040
cgtactgcgg tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc cctgaaagac 5100
gcgcagacta attcgagctc gaacaacaac aacaataaca ataacaacaa cctcgggatc 5160
gagggaagga tttcagaatt cgcggaagcg ggtatcaccg gcacgtggta caaccagcat 5220
ggttctacct tcaccgttac cgcgggtgcg gacggtaacc tgaccggtca gtacgaaaac 5280
cgtgcgcagg gcactggttg ccagaactct ccgtacaccc tgaccggtcg ttacaacggt 5340
accaaactgg aatggcgtgt tgaatggaac aactctaccg aaaactgcca ctctcgtacc 5400
gaatggcgtg gtcagtacca gggtggtgcg gaagcgcgta tcaacaccca gtggaacctg 5460
acctacgaag gtggttctgg tccggcgacc gaacagggtc aggacacctt caccaaagtt 5520
aaacaccacc accaccacca ctaagtcgac ctgcaggaag cttgtttaaa cggtctccag 5580
cttggctgtt ttg 5593
<210> 49
<211> 5593
<212> DNA
<213> Artificial Sequence
<220>
<223> pBAD_R-lib-1-18-mSA
<400> 49
gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga 60
taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact 120
cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga 180
actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 240
tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 300
gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat 360
caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg 420
tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 480
gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 540
tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 600
aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 660
cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 720
agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 780
ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 840
tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 900
tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 960
caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1020
accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1080
attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1140
ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1200
taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1260
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1320
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1380
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1440
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1500
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1560
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1620
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1680
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1740
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1800
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1860
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1920
acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1980
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2040
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2100
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2160
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2220
taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2280
cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2340
tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc 2400
atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 2460
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 2520
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 2580
aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc 2640
tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt 2700
gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa 2760
ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 2820
agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 2880
aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 2940
aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 3000
ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 3060
acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 3120
aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 3180
ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 3240
aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 3300
cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 3360
atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 3420
tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 3480
ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 3540
agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 3600
ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 3660
cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc 3720
attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 3780
cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 3840
tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 3900
tctccattct agagaaagaa gagagcctac tagatgaaaa taaaaacagg tgcacgcatc 3960
ctcgcattat ccgcattaac gacgatgatg ttttccgcct cggctctcgc caaaatcgaa 4020
gaaggtaaac tggtaatctg gattaacggc gataaaggct ataacggtct cgctgaagtc 4080
ggtaagaaat tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc ggataaactg 4140
gaagagaaat tcccacaggt tgcggcaact ggcgatggcc ctgacattat cttctgggca 4200
cacgaccgct ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa 4260
gcgttccagg acaagctgta tccgtttacc tgggatgccg tacgttacaa cggcaagctg 4320
attgcttacc cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga tctgctgccg 4380
aacccgccaa aaacctggga agagatcccg gcgctggata aagaactgaa agcgaaaggt 4440
aagagcgcgc tgatgttcaa cctgcaagaa ccgtacttca cctggccgct gattgctgct 4500
gacgggggtt atgcgttcaa gtatgaaaac ggcaagtacg acattaaaga cgtgggcgtg 4560
gataacgctg gcgcgaaagc gggtctgacc ttcctggttg acctgattaa aaacaaacac 4620
atgaatgcag acaccgatta ctccatcgca gaagctgcct ttaataaagg cgaaacagcg 4680
atgaccatca acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt gaattatggt 4740
gtaacggtac tgccgacctt caagggtcaa ccatccaaac cgttcgttgg cgtgctgagc 4800
gcaggtatta acgccgccag tccgaacaaa gagctggcaa aagagttcct cgaaaactat 4860
ctgctgactg atgaaggtct ggaagcggtt aataaagaca aaccgctggg tgccgtagcg 4920
ctgaagtctt acgaggaaga gttggcgaaa gatccacgta ttgccgccac catggaaaac 4980
gcccagaaag gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg gtatgccgtg 5040
cgtactgcgg tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc cctgaaagac 5100
gcgcagacta attcgagctc gaacaacaac aacaataaca ataacaacaa cctcgggatc 5160
gagggaagga tttcagaatt cgcggaagcg ggtatcaccg gcacgtggta caaccagcat 5220
ggttctacct tcaccgttac cgcgggtgcg gacggtaacc tgaccggtca gtacgaaaac 5280
cgtgcgcagg gcactggttg ccagaactct ccgtacaccc tgaccggtcg ttacaacggt 5340
accaaactgg aatggcgtgt tgaatggaac aactctaccg aaaactgcca ctctcgtacc 5400
gaatggcgtg gtcagtacca gggtggtgcg gaagcgcgta tcaacaccca gtggaacctg 5460
acctacgaag gtggttctgg tccggcgacc gaacagggtc aggacacctt caccaaagtt 5520
aaacaccacc accaccacca ctaagtcgac ctgcaggaag cttgtttaaa cggtctccag 5580
cttggctgtt ttg 5593
<210> 50
<211> 5594
<212> DNA
<213> Artificial Sequence
<220>
<223> pBAD_R-lib-2-2-mSA
<400> 50
gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga 60
taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact 120
cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga 180
actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 240
tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 300
gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat 360
caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg 420
tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 480
gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 540
tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 600
aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 660
cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 720
agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 780
ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 840
tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 900
tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 960
caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1020
accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1080
attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1140
ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1200
taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1260
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1320
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1380
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1440
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1500
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1560
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1620
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1680
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1740
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1800
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1860
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1920
acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1980
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2040
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2100
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2160
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2220
taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2280
cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2340
tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc 2400
atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 2460
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 2520
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 2580
aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc 2640
tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt 2700
gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa 2760
ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 2820
agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 2880
aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 2940
aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 3000
ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 3060
acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 3120
aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 3180
ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 3240
aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 3300
cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 3360
atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 3420
tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 3480
ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 3540
agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 3600
ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 3660
cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc 3720
attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 3780
cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 3840
tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 3900
tctccatgaa ccctaataca ttaggagatc ttctatgaaa ataaaaacag gtgcacgcat 3960
cctcgcatta tccgcattaa cgacgatgat gttttccgcc tcggctctcg ccaaaatcga 4020
agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt 4080
cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact 4140
ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc 4200
acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa 4260
agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct 4320
gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc 4380
gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg 4440
taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc 4500
tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt 4560
ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca 4620
catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc 4680
gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg 4740
tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag 4800
cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta 4860
tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc 4920
gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ccatggaaaa 4980
cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt 5040
gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga 5100
cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat 5160
cgagggaagg atttcagaat tcgcggaagc gggtatcacc ggcacgtggt acaaccagca 5220
tggttctacc ttcaccgtta ccgcgggtgc ggacggtaac ctgaccggtc agtacgaaaa 5280
ccgtgcgcag ggcactggtt gccagaactc tccgtacacc ctgaccggtc gttacaacgg 5340
taccaaactg gaatggcgtg ttgaatggaa caactctacc gaaaactgcc actctcgtac 5400
cgaatggcgt ggtcagtacc agggtggtgc ggaagcgcgt atcaacaccc agtggaacct 5460
gacctacgaa ggtggttctg gtccggcgac cgaacagggt caggacacct tcaccaaagt 5520
taaacaccac caccaccacc actaagtcga cctgcaggaa gcttgtttaa acggtctcca 5580
gcttggctgt tttg 5594
<210> 51
<211> 5594
<212> DNA
<213> Artificial Sequence
<220>
<223> pBAD_R-lib-2-3-mSA
<400> 51
gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga 60
taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact 120
cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga 180
actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 240
tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 300
gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat 360
caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg 420
tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 480
gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 540
tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 600
aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 660
cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 720
agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 780
ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 840
tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 900
tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 960
caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1020
accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1080
attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1140
ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1200
taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1260
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1320
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1380
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1440
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1500
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1560
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1620
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1680
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1740
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1800
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1860
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1920
acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1980
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2040
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2100
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2160
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2220
taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2280
cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2340
tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc 2400
atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 2460
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 2520
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 2580
aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc 2640
tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt 2700
gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa 2760
ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 2820
agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 2880
aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 2940
aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 3000
ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 3060
acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 3120
aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 3180
ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 3240
aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 3300
cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 3360
atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 3420
tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 3480
ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 3540
agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 3600
ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 3660
cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc 3720
attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 3780
cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 3840
tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 3900
tctccatgaa ccctaataca ttaggacata ttctatgaaa ataaaaacag gtgcacgcat 3960
cctcgcatta tccgcattaa cgacgatgat gttttccgcc tcggctctcg ccaaaatcga 4020
agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt 4080
cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact 4140
ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc 4200
acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa 4260
agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct 4320
gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc 4380
gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg 4440
taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc 4500
tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt 4560
ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca 4620
catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc 4680
gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg 4740
tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag 4800
cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta 4860
tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc 4920
gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ccatggaaaa 4980
cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt 5040
gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga 5100
cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat 5160
cgagggaagg atttcagaat tcgcggaagc gggtatcacc ggcacgtggt acaaccagca 5220
tggttctacc ttcaccgtta ccgcgggtgc ggacggtaac ctgaccggtc agtacgaaaa 5280
ccgtgcgcag ggcactggtt gccagaactc tccgtacacc ctgaccggtc gttacaacgg 5340
taccaaactg gaatggcgtg ttgaatggaa caactctacc gaaaactgcc actctcgtac 5400
cgaatggcgt ggtcagtacc agggtggtgc ggaagcgcgt atcaacaccc agtggaacct 5460
gacctacgaa ggtggttctg gtccggcgac cgaacagggt caggacacct tcaccaaagt 5520
taaacaccac caccaccacc actaagtcga cctgcaggaa gcttgtttaa acggtctcca 5580
gcttggctgt tttg 5594
<210> 52
<211> 5594
<212> DNA
<213> Artificial Sequence
<220>
<223> pBAD_R-lib-2-4-mSA
<400> 52
gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga 60
taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact 120
cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga 180
actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 240
tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 300
gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat 360
caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg 420
tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 480
gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 540
tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 600
aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 660
cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 720
agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 780
ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 840
tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 900
tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 960
caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1020
accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1080
attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1140
ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1200
taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1260
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1320
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1380
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1440
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1500
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1560
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1620
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1680
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1740
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1800
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1860
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1920
acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1980
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2040
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2100
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2160
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2220
taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2280
cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2340
tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc 2400
atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 2460
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 2520
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 2580
aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc 2640
tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt 2700
gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa 2760
ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 2820
agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 2880
aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 2940
aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 3000
ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 3060
acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 3120
aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 3180
ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 3240
aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 3300
cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 3360
atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 3420
tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 3480
ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 3540
agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 3600
ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 3660
cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc 3720
attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 3780
cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 3840
tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 3900
tctccatgaa ccctaataca ttaggacatc atctatgaaa ataaaaacag gtgcacgcat 3960
cctcgcatta tccgcattaa cgacgatgat gttttccgcc tcggctctcg ccaaaatcga 4020
agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt 4080
cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact 4140
ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc 4200
acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa 4260
agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct 4320
gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc 4380
gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg 4440
taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc 4500
tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt 4560
ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca 4620
catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc 4680
gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg 4740
tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag 4800
cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta 4860
tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc 4920
gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ccatggaaaa 4980
cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt 5040
gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga 5100
cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat 5160
cgagggaagg atttcagaat tcgcggaagc gggtatcacc ggcacgtggt acaaccagca 5220
tggttctacc ttcaccgtta ccgcgggtgc ggacggtaac ctgaccggtc agtacgaaaa 5280
ccgtgcgcag ggcactggtt gccagaactc tccgtacacc ctgaccggtc gttacaacgg 5340
taccaaactg gaatggcgtg ttgaatggaa caactctacc gaaaactgcc actctcgtac 5400
cgaatggcgt ggtcagtacc agggtggtgc ggaagcgcgt atcaacaccc agtggaacct 5460
gacctacgaa ggtggttctg gtccggcgac cgaacagggt caggacacct tcaccaaagt 5520
taaacaccac caccaccacc actaagtcga cctgcaggaa gcttgtttaa acggtctcca 5580
gcttggctgt tttg 5594
<210> 53
<211> 5594
<212> DNA
<213> Artificial Sequence
<220>
<223> pBAD_R-lib-2-5-mSA
<400> 53
gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga 60
taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact 120
cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga 180
actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 240
tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 300
gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat 360
caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg 420
tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 480
gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 540
tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 600
aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 660
cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 720
agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 780
ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 840
tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 900
tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 960
caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1020
accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1080
attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1140
ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1200
taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1260
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1320
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1380
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1440
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1500
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1560
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1620
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1680
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1740
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1800
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1860
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1920
acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1980
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2040
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2100
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2160
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2220
taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2280
cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2340
tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc 2400
atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 2460
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 2520
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 2580
aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc 2640
tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt 2700
gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa 2760
ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 2820
agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 2880
aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 2940
aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 3000
ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 3060
acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 3120
aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 3180
ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 3240
aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 3300
cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 3360
atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 3420
tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 3480
ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 3540
agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 3600
ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 3660
cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc 3720
attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 3780
cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 3840
tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 3900
tctccatgaa ccctaataca taaggagatc atatatgaaa ataaaaacag gtgcacgcat 3960
cctcgcatta tccgcattaa cgacgatgat gttttccgcc tcggctctcg ccaaaatcga 4020
agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt 4080
cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact 4140
ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc 4200
acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa 4260
agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct 4320
gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc 4380
gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg 4440
taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc 4500
tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt 4560
ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca 4620
catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc 4680
gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg 4740
tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag 4800
cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta 4860
tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc 4920
gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ccatggaaaa 4980
cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt 5040
gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga 5100
cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat 5160
cgagggaagg atttcagaat tcgcggaagc gggtatcacc ggcacgtggt acaaccagca 5220
tggttctacc ttcaccgtta ccgcgggtgc ggacggtaac ctgaccggtc agtacgaaaa 5280
ccgtgcgcag ggcactggtt gccagaactc tccgtacacc ctgaccggtc gttacaacgg 5340
taccaaactg gaatggcgtg ttgaatggaa caactctacc gaaaactgcc actctcgtac 5400
cgaatggcgt ggtcagtacc agggtggtgc ggaagcgcgt atcaacaccc agtggaacct 5460
gacctacgaa ggtggttctg gtccggcgac cgaacagggt caggacacct tcaccaaagt 5520
taaacaccac caccaccacc actaagtcga cctgcaggaa gcttgtttaa acggtctcca 5580
gcttggctgt tttg 5594
<210> 54
<211> 5594
<212> DNA
<213> Artificial Sequence
<220>
<223> pBAD_R-lib-2-6-mSA
<400> 54
gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga 60
taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact 120
cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga 180
actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 240
tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 300
gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat 360
caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg 420
tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 480
gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 540
tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 600
aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 660
cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 720
agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 780
ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 840
tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 900
tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 960
caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1020
accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1080
attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1140
ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1200
taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1260
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1320
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1380
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1440
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1500
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1560
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1620
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1680
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1740
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1800
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1860
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1920
acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1980
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2040
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2100
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2160
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2220
taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2280
cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2340
tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc 2400
atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 2460
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 2520
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 2580
aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc 2640
tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt 2700
gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa 2760
ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 2820
agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 2880
aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 2940
aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 3000
ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 3060
acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 3120
aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 3180
ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 3240
aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 3300
cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 3360
atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 3420
tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 3480
ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 3540
agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 3600
ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 3660
cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc 3720
attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 3780
cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 3840
tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 3900
tctccatgaa ccctaataca taaggacata atatatgaaa ataaaaacag gtgcacgcat 3960
cctcgcatta tccgcattaa cgacgatgat gttttccgcc tcggctctcg ccaaaatcga 4020
agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt 4080
cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact 4140
ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc 4200
acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa 4260
agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct 4320
gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc 4380
gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg 4440
taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc 4500
tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt 4560
ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca 4620
catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc 4680
gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg 4740
tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag 4800
cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta 4860
tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc 4920
gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ccatggaaaa 4980
cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt 5040
gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga 5100
cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat 5160
cgagggaagg atttcagaat tcgcggaagc gggtatcacc ggcacgtggt acaaccagca 5220
tggttctacc ttcaccgtta ccgcgggtgc ggacggtaac ctgaccggtc agtacgaaaa 5280
ccgtgcgcag ggcactggtt gccagaactc tccgtacacc ctgaccggtc gttacaacgg 5340
taccaaactg gaatggcgtg ttgaatggaa caactctacc gaaaactgcc actctcgtac 5400
cgaatggcgt ggtcagtacc agggtggtgc ggaagcgcgt atcaacaccc agtggaacct 5460
gacctacgaa ggtggttctg gtccggcgac cgaacagggt caggacacct tcaccaaagt 5520
taaacaccac caccaccacc actaagtcga cctgcaggaa gcttgtttaa acggtctcca 5580
gcttggctgt tttg 5594
<210> 55
<211> 5594
<212> DNA
<213> Artificial Sequence
<220>
<223> pBAD_R-lib-2-7-mSA
<400> 55
gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga 60
taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact 120
cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga 180
actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 240
tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 300
gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat 360
caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg 420
tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 480
gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 540
tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 600
aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 660
cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 720
agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 780
ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 840
tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 900
tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 960
caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1020
accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1080
attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1140
ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1200
taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1260
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1320
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1380
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1440
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1500
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1560
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1620
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1680
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1740
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1800
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1860
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1920
acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1980
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2040
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2100
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2160
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2220
taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2280
cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2340
tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc 2400
atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 2460
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 2520
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 2580
aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc 2640
tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt 2700
gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa 2760
ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 2820
agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 2880
aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 2940
aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 3000
ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 3060
acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 3120
aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 3180
ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 3240
aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 3300
cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 3360
atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 3420
tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 3480
ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 3540
agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 3600
ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 3660
cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc 3720
attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 3780
cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 3840
tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 3900
tctccatgaa ccctaataca taaggagatt atctatgaaa ataaaaacag gtgcacgcat 3960
cctcgcatta tccgcattaa cgacgatgat gttttccgcc tcggctctcg ccaaaatcga 4020
agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt 4080
cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact 4140
ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc 4200
acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa 4260
agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct 4320
gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc 4380
gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg 4440
taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc 4500
tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt 4560
ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca 4620
catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc 4680
gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg 4740
tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag 4800
cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta 4860
tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc 4920
gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ccatggaaaa 4980
cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt 5040
gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga 5100
cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat 5160
cgagggaagg atttcagaat tcgcggaagc gggtatcacc ggcacgtggt acaaccagca 5220
tggttctacc ttcaccgtta ccgcgggtgc ggacggtaac ctgaccggtc agtacgaaaa 5280
ccgtgcgcag ggcactggtt gccagaactc tccgtacacc ctgaccggtc gttacaacgg 5340
taccaaactg gaatggcgtg ttgaatggaa caactctacc gaaaactgcc actctcgtac 5400
cgaatggcgt ggtcagtacc agggtggtgc ggaagcgcgt atcaacaccc agtggaacct 5460
gacctacgaa ggtggttctg gtccggcgac cgaacagggt caggacacct tcaccaaagt 5520
taaacaccac caccaccacc actaagtcga cctgcaggaa gcttgtttaa acggtctcca 5580
gcttggctgt tttg 5594
<210> 56
<211> 5594
<212> DNA
<213> Artificial Sequence
<220>
<223> pBAD_R-lib-2-8-mSA
<400> 56
gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga 60
taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact 120
cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga 180
actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 240
tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 300
gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat 360
caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg 420
tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 480
gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 540
tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 600
aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 660
cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 720
agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 780
ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 840
tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 900
tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 960
caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1020
accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1080
attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1140
ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1200
taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1260
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1320
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1380
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1440
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1500
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1560
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1620
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1680
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1740
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1800
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1860
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1920
acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1980
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2040
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2100
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2160
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2220
taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2280
cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2340
tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc 2400
atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 2460
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 2520
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 2580
aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc 2640
tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt 2700
gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa 2760
ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 2820
agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 2880
aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 2940
aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 3000
ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 3060
acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 3120
aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 3180
ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 3240
aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 3300
cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 3360
atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 3420
tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 3480
ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 3540
agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 3600
ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 3660
cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc 3720
attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 3780
cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 3840
tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 3900
tctccatgaa ccctaataca ttaggagatt atatatgaaa ataaaaacag gtgcacgcat 3960
cctcgcatta tccgcattaa cgacgatgat gttttccgcc tcggctctcg ccaaaatcga 4020
agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt 4080
cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact 4140
ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc 4200
acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa 4260
agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct 4320
gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc 4380
gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg 4440
taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc 4500
tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt 4560
ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca 4620
catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc 4680
gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg 4740
tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag 4800
cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta 4860
tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc 4920
gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ccatggaaaa 4980
cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt 5040
gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga 5100
cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat 5160
cgagggaagg atttcagaat tcgcggaagc gggtatcacc ggcacgtggt acaaccagca 5220
tggttctacc ttcaccgtta ccgcgggtgc ggacggtaac ctgaccggtc agtacgaaaa 5280
ccgtgcgcag ggcactggtt gccagaactc tccgtacacc ctgaccggtc gttacaacgg 5340
taccaaactg gaatggcgtg ttgaatggaa caactctacc gaaaactgcc actctcgtac 5400
cgaatggcgt ggtcagtacc agggtggtgc ggaagcgcgt atcaacaccc agtggaacct 5460
gacctacgaa ggtggttctg gtccggcgac cgaacagggt caggacacct tcaccaaagt 5520
taaacaccac caccaccacc actaagtcga cctgcaggaa gcttgtttaa acggtctcca 5580
gcttggctgt tttg 5594
<210> 57
<211> 5594
<212> DNA
<213> Artificial Sequence
<220>
<223> pBAD_R-lib-2-14-mSA
<400> 57
gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga 60
taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact 120
cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga 180
actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 240
tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 300
gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat 360
caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg 420
tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 480
gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 540
tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 600
aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 660
cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 720
agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 780
ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 840
tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 900
tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 960
caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1020
accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1080
attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1140
ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1200
taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1260
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1320
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1380
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1440
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1500
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1560
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1620
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1680
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1740
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1800
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1860
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1920
acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1980
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2040
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2100
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2160
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2220
taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2280
cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2340
tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc 2400
atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 2460
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 2520
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 2580
aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc 2640
tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt 2700
gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa 2760
ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 2820
agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 2880
aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 2940
aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 3000
ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 3060
acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 3120
aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 3180
ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 3240
aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 3300
cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 3360
atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 3420
tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 3480
ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 3540
agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 3600
ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 3660
cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc 3720
attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 3780
cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 3840
tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 3900
tctccatgaa ccctaataca taaggacatc ttatatgaaa ataaaaacag gtgcacgcat 3960
cctcgcatta tccgcattaa cgacgatgat gttttccgcc tcggctctcg ccaaaatcga 4020
agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt 4080
cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact 4140
ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc 4200
acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa 4260
agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct 4320
gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc 4380
gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg 4440
taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc 4500
tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt 4560
ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca 4620
catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc 4680
gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg 4740
tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag 4800
cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta 4860
tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc 4920
gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ccatggaaaa 4980
cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt 5040
gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga 5100
cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat 5160
cgagggaagg atttcagaat tcgcggaagc gggtatcacc ggcacgtggt acaaccagca 5220
tggttctacc ttcaccgtta ccgcgggtgc ggacggtaac ctgaccggtc agtacgaaaa 5280
ccgtgcgcag ggcactggtt gccagaactc tccgtacacc ctgaccggtc gttacaacgg 5340
taccaaactg gaatggcgtg ttgaatggaa caactctacc gaaaactgcc actctcgtac 5400
cgaatggcgt ggtcagtacc agggtggtgc ggaagcgcgt atcaacaccc agtggaacct 5460
gacctacgaa ggtggttctg gtccggcgac cgaacagggt caggacacct tcaccaaagt 5520
taaacaccac caccaccacc actaagtcga cctgcaggaa gcttgtttaa acggtctcca 5580
gcttggctgt tttg 5594
<210> 58
<211> 5594
<212> DNA
<213> Artificial Sequence
<220>
<223> pBAD_R-lib-2-16-mSA
<400> 58
gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga 60
taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact 120
cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga 180
actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 240
tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 300
gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat 360
caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg 420
tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 480
gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 540
tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 600
aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 660
cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 720
agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 780
ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 840
tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 900
tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 960
caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1020
accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1080
attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1140
ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1200
taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1260
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1320
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1380
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1440
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1500
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1560
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1620
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1680
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1740
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1800
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1860
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1920
acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1980
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2040
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2100
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2160
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2220
taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2280
cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2340
tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc 2400
atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 2460
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 2520
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 2580
aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc 2640
tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt 2700
gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa 2760
ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 2820
agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 2880
aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 2940
aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 3000
ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 3060
acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 3120
aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 3180
ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 3240
aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 3300
cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 3360
atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 3420
tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 3480
ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 3540
agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 3600
ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 3660
cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc 3720
attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 3780
cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 3840
tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 3900
tctccatgaa cactaataca ttaggagatc ttctatgaaa ataaaaacag gtgcacgcat 3960
cctcgcatta tccgcattaa cgacgatgat gttttccgcc tcggctctcg ccaaaatcga 4020
agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt 4080
cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact 4140
ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc 4200
acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa 4260
agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct 4320
gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc 4380
gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg 4440
taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc 4500
tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt 4560
ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca 4620
catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc 4680
gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg 4740
tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag 4800
cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta 4860
tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc 4920
gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ccatggaaaa 4980
cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt 5040
gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga 5100
cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat 5160
cgagggaagg atttcagaat tcgcggaagc gggtatcacc ggcacgtggt acaaccagca 5220
tggttctacc ttcaccgtta ccgcgggtgc ggacggtaac ctgaccggtc agtacgaaaa 5280
ccgtgcgcag ggcactggtt gccagaactc tccgtacacc ctgaccggtc gttacaacgg 5340
taccaaactg gaatggcgtg ttgaatggaa caactctacc gaaaactgcc actctcgtac 5400
cgaatggcgt ggtcagtacc agggtggtgc ggaagcgcgt atcaacaccc agtggaacct 5460
gacctacgaa ggtggttctg gtccggcgac cgaacagggt caggacacct tcaccaaagt 5520
taaacaccac caccaccacc actaagtcga cctgcaggaa gcttgtttaa acggtctcca 5580
gcttggctgt tttg 5594
<210> 59
<211> 5594
<212> DNA
<213> Artificial Sequence
<220>
<223> pBAD_R-lib-2-17-mSA
<400> 59
gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga 60
taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact 120
cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga 180
actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 240
tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 300
gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat 360
caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg 420
tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 480
gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 540
tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 600
aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 660
cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 720
agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 780
ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 840
tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 900
tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 960
caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1020
accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1080
attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1140
ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1200
taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1260
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1320
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1380
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1440
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1500
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1560
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1620
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1680
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1740
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1800
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1860
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1920
acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1980
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2040
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2100
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2160
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2220
taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2280
cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2340
tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc 2400
atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 2460
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 2520
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 2580
aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc 2640
tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt 2700
gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa 2760
ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 2820
agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 2880
aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 2940
aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 3000
ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 3060
acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 3120
aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 3180
ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 3240
aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 3300
cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 3360
atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 3420
tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 3480
ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 3540
agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 3600
ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 3660
cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc 3720
attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 3780
cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 3840
tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 3900
tctccatgaa cactaataca taaggacata atatatgaaa ataaaaacag gtgcacgcat 3960
cctcgcatta tccgcattaa cgacgatgat gttttccgcc tcggctctcg ccaaaatcga 4020
agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt 4080
cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact 4140
ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc 4200
acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa 4260
agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct 4320
gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc 4380
gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg 4440
taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc 4500
tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt 4560
ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca 4620
catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc 4680
gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg 4740
tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag 4800
cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta 4860
tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc 4920
gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ccatggaaaa 4980
cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt 5040
gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga 5100
cgcgcagact aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat 5160
cgagggaagg atttcagaat tcgcggaagc gggtatcacc ggcacgtggt acaaccagca 5220
tggttctacc ttcaccgtta ccgcgggtgc ggacggtaac ctgaccggtc agtacgaaaa 5280
ccgtgcgcag ggcactggtt gccagaactc tccgtacacc ctgaccggtc gttacaacgg 5340
taccaaactg gaatggcgtg ttgaatggaa caactctacc gaaaactgcc actctcgtac 5400
cgaatggcgt ggtcagtacc agggtggtgc ggaagcgcgt atcaacaccc agtggaacct 5460
gacctacgaa ggtggttctg gtccggcgac cgaacagggt caggacacct tcaccaaagt 5520
taaacaccac caccaccacc actaagtcga cctgcaggaa gcttgtttaa acggtctcca 5580
gcttggctgt tttg 5594
<210> 60
<211> 5599
<212> DNA
<213> Artificial Sequence
<220>
<223> pBAD_R-lib-3-4-mSA
<400> 60
gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga 60
taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact 120
cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga 180
actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 240
tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 300
gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat 360
caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg 420
tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 480
gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 540
tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 600
aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 660
cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 720
agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 780
ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 840
tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 900
tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 960
caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1020
accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1080
attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1140
ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1200
taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1260
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1320
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1380
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1440
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1500
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1560
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1620
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1680
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1740
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1800
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1860
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1920
acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1980
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2040
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2100
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2160
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2220
taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2280
cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2340
tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc 2400
atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 2460
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 2520
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 2580
aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc 2640
tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt 2700
gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa 2760
ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 2820
agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 2880
aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 2940
aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 3000
ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 3060
acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 3120
aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 3180
ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 3240
aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 3300
cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 3360
atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 3420
tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 3480
ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 3540
agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 3600
ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 3660
cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc 3720
attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 3780
cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 3840
tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 3900
tctccattta agtagttaaa cagggtatat aggggaagaa tgaaaataaa aacaggtgca 3960
cgcatcctcg cattatccgc attaacgacg atgatgtttt ccgcctcggc tctcgccaaa 4020
atcgaagaag gtaaactggt aatctggatt aacggcgata aaggctataa cggtctcgct 4080
gaagtcggta agaaattcga gaaagatacc ggaattaaag tcaccgttga gcatccggat 4140
aaactggaag agaaattccc acaggttgcg gcaactggcg atggccctga cattatcttc 4200
tgggcacacg accgctttgg tggctacgct caatctggcc tgttggctga aatcaccccg 4260
gacaaagcgt tccaggacaa gctgtatccg tttacctggg atgccgtacg ttacaacggc 4320
aagctgattg cttacccgat cgctgttgaa gcgttatcgc tgatttataa caaagatctg 4380
ctgccgaacc cgccaaaaac ctgggaagag atcccggcgc tggataaaga actgaaagcg 4440
aaaggtaaga gcgcgctgat gttcaacctg caagaaccgt acttcacctg gccgctgatt 4500
gctgctgacg ggggttatgc gttcaagtat gaaaacggca agtacgacat taaagacgtg 4560
ggcgtggata acgctggcgc gaaagcgggt ctgaccttcc tggttgacct gattaaaaac 4620
aaacacatga atgcagacac cgattactcc atcgcagaag ctgcctttaa taaaggcgaa 4680
acagcgatga ccatcaacgg cccgtgggca tggtccaaca tcgacaccag caaagtgaat 4740
tatggtgtaa cggtactgcc gaccttcaag ggtcaaccat ccaaaccgtt cgttggcgtg 4800
ctgagcgcag gtattaacgc cgccagtccg aacaaagagc tggcaaaaga gttcctcgaa 4860
aactatctgc tgactgatga aggtctggaa gcggttaata aagacaaacc gctgggtgcc 4920
gtagcgctga agtcttacga ggaagagttg gcgaaagatc cacgtattgc cgccaccatg 4980
gaaaacgccc agaaaggtga aatcatgccg aacatcccgc agatgtccgc tttctggtat 5040
gccgtgcgta ctgcggtgat caacgccgcc agcggtcgtc agactgtcga tgaagccctg 5100
aaagacgcgc agactaattc gagctcgaac aacaacaaca ataacaataa caacaacctc 5160
gggatcgagg gaaggatttc agaattcgcg gaagcgggta tcaccggcac gtggtacaac 5220
cagcatggtt ctaccttcac cgttaccgcg ggtgcggacg gtaacctgac cggtcagtac 5280
gaaaaccgtg cgcagggcac tggttgccag aactctccgt acaccctgac cggtcgttac 5340
aacggtacca aactggaatg gcgtgttgaa tggaacaact ctaccgaaaa ctgccactct 5400
cgtaccgaat ggcgtggtca gtaccagggt ggtgcggaag cgcgtatcaa cacccagtgg 5460
aacctgacct acgaaggtgg ttctggtccg gcgaccgaac agggtcagga caccttcacc 5520
aaagttaaac accaccacca ccaccactaa gtcgacctgc aggaagcttg tttaaacggt 5580
ctccagcttg gctgttttg 5599
<210> 61
<211> 5599
<212> DNA
<213> Artificial Sequence
<220>
<223> pBAD_R-lib-3-5-mSA
<400> 61
gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga 60
taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact 120
cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga 180
actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 240
tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 300
gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat 360
caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg 420
tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 480
gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 540
tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 600
aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 660
cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 720
agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 780
ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 840
tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 900
tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 960
caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1020
accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1080
attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1140
ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1200
taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1260
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1320
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1380
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1440
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1500
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1560
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1620
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1680
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1740
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1800
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1860
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1920
acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1980
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2040
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2100
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2160
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2220
taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2280
cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2340
tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc 2400
atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 2460
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 2520
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 2580
aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc 2640
tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt 2700
gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa 2760
ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 2820
agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 2880
aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 2940
aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 3000
ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 3060
acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 3120
aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 3180
ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 3240
aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 3300
cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 3360
atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 3420
tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 3480
ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 3540
agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 3600
ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 3660
cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc 3720
attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 3780
cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 3840
tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 3900
tctccattta agtagttaaa cagggtatat aggacgagaa tgaaaataaa aacaggtgca 3960
cgcatcctcg cattatccgc attaacgacg atgatgtttt ccgcctcggc tctcgccaaa 4020
atcgaagaag gtaaactggt aatctggatt aacggcgata aaggctataa cggtctcgct 4080
gaagtcggta agaaattcga gaaagatacc ggaattaaag tcaccgttga gcatccggat 4140
aaactggaag agaaattccc acaggttgcg gcaactggcg atggccctga cattatcttc 4200
tgggcacacg accgctttgg tggctacgct caatctggcc tgttggctga aatcaccccg 4260
gacaaagcgt tccaggacaa gctgtatccg tttacctggg atgccgtacg ttacaacggc 4320
aagctgattg cttacccgat cgctgttgaa gcgttatcgc tgatttataa caaagatctg 4380
ctgccgaacc cgccaaaaac ctgggaagag atcccggcgc tggataaaga actgaaagcg 4440
aaaggtaaga gcgcgctgat gttcaacctg caagaaccgt acttcacctg gccgctgatt 4500
gctgctgacg ggggttatgc gttcaagtat gaaaacggca agtacgacat taaagacgtg 4560
ggcgtggata acgctggcgc gaaagcgggt ctgaccttcc tggttgacct gattaaaaac 4620
aaacacatga atgcagacac cgattactcc atcgcagaag ctgcctttaa taaaggcgaa 4680
acagcgatga ccatcaacgg cccgtgggca tggtccaaca tcgacaccag caaagtgaat 4740
tatggtgtaa cggtactgcc gaccttcaag ggtcaaccat ccaaaccgtt cgttggcgtg 4800
ctgagcgcag gtattaacgc cgccagtccg aacaaagagc tggcaaaaga gttcctcgaa 4860
aactatctgc tgactgatga aggtctggaa gcggttaata aagacaaacc gctgggtgcc 4920
gtagcgctga agtcttacga ggaagagttg gcgaaagatc cacgtattgc cgccaccatg 4980
gaaaacgccc agaaaggtga aatcatgccg aacatcccgc agatgtccgc tttctggtat 5040
gccgtgcgta ctgcggtgat caacgccgcc agcggtcgtc agactgtcga tgaagccctg 5100
aaagacgcgc agactaattc gagctcgaac aacaacaaca ataacaataa caacaacctc 5160
gggatcgagg gaaggatttc agaattcgcg gaagcgggta tcaccggcac gtggtacaac 5220
cagcatggtt ctaccttcac cgttaccgcg ggtgcggacg gtaacctgac cggtcagtac 5280
gaaaaccgtg cgcagggcac tggttgccag aactctccgt acaccctgac cggtcgttac 5340
aacggtacca aactggaatg gcgtgttgaa tggaacaact ctaccgaaaa ctgccactct 5400
cgtaccgaat ggcgtggtca gtaccagggt ggtgcggaag cgcgtatcaa cacccagtgg 5460
aacctgacct acgaaggtgg ttctggtccg gcgaccgaac agggtcagga caccttcacc 5520
aaagttaaac accaccacca ccaccactaa gtcgacctgc aggaagcttg tttaaacggt 5580
ctccagcttg gctgttttg 5599
<210> 62
<211> 5599
<212> DNA
<213> Artificial Sequence
<220>
<223> pBAD_R-lib-3-11-mSA
<400> 62
gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga 60
taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact 120
cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga 180
actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 240
tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 300
gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat 360
caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg 420
tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 480
gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 540
tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 600
aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 660
cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 720
agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 780
ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 840
tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 900
tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 960
caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1020
accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1080
attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1140
ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1200
taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1260
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1320
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1380
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1440
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1500
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1560
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1620
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1680
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1740
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1800
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1860
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1920
acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1980
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2040
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2100
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2160
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2220
taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2280
cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2340
tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc 2400
atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 2460
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 2520
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 2580
aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc 2640
tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt 2700
gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa 2760
ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 2820
agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 2880
aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 2940
aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 3000
ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 3060
acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 3120
aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 3180
ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 3240
aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 3300
cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 3360
atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 3420
tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 3480
ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 3540
agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 3600
ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 3660
cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc 3720
attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 3780
cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 3840
tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 3900
tctccattta agtagttaaa cagggtatat agggctataa tgaaaataaa aacaggtgca 3960
cgcatcctcg cattatccgc attaacgacg atgatgtttt ccgcctcggc tctcgccaaa 4020
atcgaagaag gtaaactggt aatctggatt aacggcgata aaggctataa cggtctcgct 4080
gaagtcggta agaaattcga gaaagatacc ggaattaaag tcaccgttga gcatccggat 4140
aaactggaag agaaattccc acaggttgcg gcaactggcg atggccctga cattatcttc 4200
tgggcacacg accgctttgg tggctacgct caatctggcc tgttggctga aatcaccccg 4260
gacaaagcgt tccaggacaa gctgtatccg tttacctggg atgccgtacg ttacaacggc 4320
aagctgattg cttacccgat cgctgttgaa gcgttatcgc tgatttataa caaagatctg 4380
ctgccgaacc cgccaaaaac ctgggaagag atcccggcgc tggataaaga actgaaagcg 4440
aaaggtaaga gcgcgctgat gttcaacctg caagaaccgt acttcacctg gccgctgatt 4500
gctgctgacg ggggttatgc gttcaagtat gaaaacggca agtacgacat taaagacgtg 4560
ggcgtggata acgctggcgc gaaagcgggt ctgaccttcc tggttgacct gattaaaaac 4620
aaacacatga atgcagacac cgattactcc atcgcagaag ctgcctttaa taaaggcgaa 4680
acagcgatga ccatcaacgg cccgtgggca tggtccaaca tcgacaccag caaagtgaat 4740
tatggtgtaa cggtactgcc gaccttcaag ggtcaaccat ccaaaccgtt cgttggcgtg 4800
ctgagcgcag gtattaacgc cgccagtccg aacaaagagc tggcaaaaga gttcctcgaa 4860
aactatctgc tgactgatga aggtctggaa gcggttaata aagacaaacc gctgggtgcc 4920
gtagcgctga agtcttacga ggaagagttg gcgaaagatc cacgtattgc cgccaccatg 4980
gaaaacgccc agaaaggtga aatcatgccg aacatcccgc agatgtccgc tttctggtat 5040
gccgtgcgta ctgcggtgat caacgccgcc agcggtcgtc agactgtcga tgaagccctg 5100
aaagacgcgc agactaattc gagctcgaac aacaacaaca ataacaataa caacaacctc 5160
gggatcgagg gaaggatttc agaattcgcg gaagcgggta tcaccggcac gtggtacaac 5220
cagcatggtt ctaccttcac cgttaccgcg ggtgcggacg gtaacctgac cggtcagtac 5280
gaaaaccgtg cgcagggcac tggttgccag aactctccgt acaccctgac cggtcgttac 5340
aacggtacca aactggaatg gcgtgttgaa tggaacaact ctaccgaaaa ctgccactct 5400
cgtaccgaat ggcgtggtca gtaccagggt ggtgcggaag cgcgtatcaa cacccagtgg 5460
aacctgacct acgaaggtgg ttctggtccg gcgaccgaac agggtcagga caccttcacc 5520
aaagttaaac accaccacca ccaccactaa gtcgacctgc aggaagcttg tttaaacggt 5580
ctccagcttg gctgttttg 5599
<210> 63
<211> 5599
<212> DNA
<213> Artificial Sequence
<220>
<223> pBAD_R-lib-3-13-mSA
<400> 63
gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga 60
taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact 120
cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga 180
actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 240
tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 300
gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat 360
caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg 420
tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 480
gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 540
tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 600
aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 660
cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 720
agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 780
ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 840
tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 900
tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 960
caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1020
accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1080
attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1140
ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1200
taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1260
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1320
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1380
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1440
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1500
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1560
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1620
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1680
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1740
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1800
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1860
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1920
acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1980
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2040
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2100
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2160
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2220
taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2280
cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2340
tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc 2400
atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 2460
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 2520
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 2580
aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc 2640
tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt 2700
gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa 2760
ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 2820
agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 2880
aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 2940
aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 3000
ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 3060
acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 3120
aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 3180
ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 3240
aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 3300
cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 3360
atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 3420
tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 3480
ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 3540
agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 3600
ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 3660
cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc 3720
attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 3780
cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 3840
tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 3900
tctccattta agtagttaaa cagggtatat aggaggataa tgaaaataaa aacaggtgca 3960
cgcatcctcg cattatccgc attaacgacg atgatgtttt ccgcctcggc tctcgccaaa 4020
atcgaagaag gtaaactggt aatctggatt aacggcgata aaggctataa cggtctcgct 4080
gaagtcggta agaaattcga gaaagatacc ggaattaaag tcaccgttga gcatccggat 4140
aaactggaag agaaattccc acaggttgcg gcaactggcg atggccctga cattatcttc 4200
tgggcacacg accgctttgg tggctacgct caatctggcc tgttggctga aatcaccccg 4260
gacaaagcgt tccaggacaa gctgtatccg tttacctggg atgccgtacg ttacaacggc 4320
aagctgattg cttacccgat cgctgttgaa gcgttatcgc tgatttataa caaagatctg 4380
ctgccgaacc cgccaaaaac ctgggaagag atcccggcgc tggataaaga actgaaagcg 4440
aaaggtaaga gcgcgctgat gttcaacctg caagaaccgt acttcacctg gccgctgatt 4500
gctgctgacg ggggttatgc gttcaagtat gaaaacggca agtacgacat taaagacgtg 4560
ggcgtggata acgctggcgc gaaagcgggt ctgaccttcc tggttgacct gattaaaaac 4620
aaacacatga atgcagacac cgattactcc atcgcagaag ctgcctttaa taaaggcgaa 4680
acagcgatga ccatcaacgg cccgtgggca tggtccaaca tcgacaccag caaagtgaat 4740
tatggtgtaa cggtactgcc gaccttcaag ggtcaaccat ccaaaccgtt cgttggcgtg 4800
ctgagcgcag gtattaacgc cgccagtccg aacaaagagc tggcaaaaga gttcctcgaa 4860
aactatctgc tgactgatga aggtctggaa gcggttaata aagacaaacc gctgggtgcc 4920
gtagcgctga agtcttacga ggaagagttg gcgaaagatc cacgtattgc cgccaccatg 4980
gaaaacgccc agaaaggtga aatcatgccg aacatcccgc agatgtccgc tttctggtat 5040
gccgtgcgta ctgcggtgat caacgccgcc agcggtcgtc agactgtcga tgaagccctg 5100
aaagacgcgc agactaattc gagctcgaac aacaacaaca ataacaataa caacaacctc 5160
gggatcgagg gaaggatttc agaattcgcg gaagcgggta tcaccggcac gtggtacaac 5220
cagcatggtt ctaccttcac cgttaccgcg ggtgcggacg gtaacctgac cggtcagtac 5280
gaaaaccgtg cgcagggcac tggttgccag aactctccgt acaccctgac cggtcgttac 5340
aacggtacca aactggaatg gcgtgttgaa tggaacaact ctaccgaaaa ctgccactct 5400
cgtaccgaat ggcgtggtca gtaccagggt ggtgcggaag cgcgtatcaa cacccagtgg 5460
aacctgacct acgaaggtgg ttctggtccg gcgaccgaac agggtcagga caccttcacc 5520
aaagttaaac accaccacca ccaccactaa gtcgacctgc aggaagcttg tttaaacggt 5580
ctccagcttg gctgttttg 5599
<210> 64
<211> 5599
<212> DNA
<213> Artificial Sequence
<220>
<223> pBAD_R-lib-3-18-mSA
<400> 64
gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga 60
taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact 120
cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga 180
actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 240
tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 300
gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat 360
caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg 420
tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 480
gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 540
tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 600
aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 660
cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 720
agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 780
ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 840
tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 900
tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 960
caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1020
accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1080
attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1140
ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1200
taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1260
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1320
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1380
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1440
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1500
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1560
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1620
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1680
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1740
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1800
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1860
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1920
acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1980
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2040
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2100
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2160
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2220
taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2280
cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2340
tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc 2400
atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 2460
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 2520
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 2580
aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc 2640
tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt 2700
gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa 2760
ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 2820
agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 2880
aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 2940
aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 3000
ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 3060
acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 3120
aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 3180
ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 3240
aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 3300
cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 3360
atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 3420
tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 3480
ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 3540
agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 3600
ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 3660
cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc 3720
attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 3780
cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 3840
tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 3900
tctccattta agtagttaaa cagggtatat agggcgataa tgaaaataaa aacaggtgca 3960
cgcatcctcg cattatccgc attaacgacg atgatgtttt ccgcctcggc tctcgccaaa 4020
atcgaagaag gtaaactggt aatctggatt aacggcgata aaggctataa cggtctcgct 4080
gaagtcggta agaaattcga gaaagatacc ggaattaaag tcaccgttga gcatccggat 4140
aaactggaag agaaattccc acaggttgcg gcaactggcg atggccctga cattatcttc 4200
tgggcacacg accgctttgg tggctacgct caatctggcc tgttggctga aatcaccccg 4260
gacaaagcgt tccaggacaa gctgtatccg tttacctggg atgccgtacg ttacaacggc 4320
aagctgattg cttacccgat cgctgttgaa gcgttatcgc tgatttataa caaagatctg 4380
ctgccgaacc cgccaaaaac ctgggaagag atcccggcgc tggataaaga actgaaagcg 4440
aaaggtaaga gcgcgctgat gttcaacctg caagaaccgt acttcacctg gccgctgatt 4500
gctgctgacg ggggttatgc gttcaagtat gaaaacggca agtacgacat taaagacgtg 4560
ggcgtggata acgctggcgc gaaagcgggt ctgaccttcc tggttgacct gattaaaaac 4620
aaacacatga atgcagacac cgattactcc atcgcagaag ctgcctttaa taaaggcgaa 4680
acagcgatga ccatcaacgg cccgtgggca tggtccaaca tcgacaccag caaagtgaat 4740
tatggtgtaa cggtactgcc gaccttcaag ggtcaaccat ccaaaccgtt cgttggcgtg 4800
ctgagcgcag gtattaacgc cgccagtccg aacaaagagc tggcaaaaga gttcctcgaa 4860
aactatctgc tgactgatga aggtctggaa gcggttaata aagacaaacc gctgggtgcc 4920
gtagcgctga agtcttacga ggaagagttg gcgaaagatc cacgtattgc cgccaccatg 4980
gaaaacgccc agaaaggtga aatcatgccg aacatcccgc agatgtccgc tttctggtat 5040
gccgtgcgta ctgcggtgat caacgccgcc agcggtcgtc agactgtcga tgaagccctg 5100
aaagacgcgc agactaattc gagctcgaac aacaacaaca ataacaataa caacaacctc 5160
gggatcgagg gaaggatttc agaattcgcg gaagcgggta tcaccggcac gtggtacaac 5220
cagcatggtt ctaccttcac cgttaccgcg ggtgcggacg gtaacctgac cggtcagtac 5280
gaaaaccgtg cgcagggcac tggttgccag aactctccgt acaccctgac cggtcgttac 5340
aacggtacca aactggaatg gcgtgttgaa tggaacaact ctaccgaaaa ctgccactct 5400
cgtaccgaat ggcgtggtca gtaccagggt ggtgcggaag cgcgtatcaa cacccagtgg 5460
aacctgacct acgaaggtgg ttctggtccg gcgaccgaac agggtcagga caccttcacc 5520
aaagttaaac accaccacca ccaccactaa gtcgacctgc aggaagcttg tttaaacggt 5580
ctccagcttg gctgttttg 5599
<210> 65
<211> 5599
<212> DNA
<213> Artificial Sequence
<220>
<223> pBAD_R-lib-3-20-mSA
<400> 65
gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga 60
taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact 120
cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga 180
actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 240
tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac 300
gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat 360
caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg 420
tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 480
gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 540
tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 600
aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 660
cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 720
agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 780
ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 840
tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 900
tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 960
caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1020
accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1080
attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1140
ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1200
taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1260
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1320
aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1380
agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1440
ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1500
ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1560
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1620
tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1680
tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1740
tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1800
tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 1860
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 1920
acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 1980
ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2040
gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2100
ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2160
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2220
taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2280
cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2340
tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc 2400
atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga 2460
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 2520
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 2580
aaacgcgcga ggcagcagat caattcgcgc gcgaaggcga agcggcatgc ataatgtgcc 2640
tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt 2700
gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa 2760
ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag 2820
agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc 2880
aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct 2940
aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg 3000
ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt 3060
acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac 3120
aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg 3180
ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag 3240
aaccccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga 3300
cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc gtagtgatga 3360
atctctcctg gcgggaacag caaaatatca cccggtcggc aaacaaattc tcgtccctga 3420
tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc 3480
ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc 3540
agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt 3600
ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt 3660
cactgcgtct tttactggct cttctcgcta accaaaccgg taaccccgct tattaaaagc 3720
attctgtaac aaagcgggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat 3780
cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca 3840
tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt 3900
tctccattta agtaattaaa cagggtatat aggggaagaa tgaaaataaa aacaggtgca 3960
cgcatcctcg cattatccgc attaacgacg atgatgtttt ccgcctcggc tctcgccaaa 4020
atcgaagaag gtaaactggt aatctggatt aacggcgata aaggctataa cggtctcgct 4080
gaagtcggta agaaattcga gaaagatacc ggaattaaag tcaccgttga gcatccggat 4140
aaactggaag agaaattccc acaggttgcg gcaactggcg atggccctga cattatcttc 4200
tgggcacacg accgctttgg tggctacgct caatctggcc tgttggctga aatcaccccg 4260
gacaaagcgt tccaggacaa gctgtatccg tttacctggg atgccgtacg ttacaacggc 4320
aagctgattg cttacccgat cgctgttgaa gcgttatcgc tgatttataa caaagatctg 4380
ctgccgaacc cgccaaaaac ctgggaagag atcccggcgc tggataaaga actgaaagcg 4440
aaaggtaaga gcgcgctgat gttcaacctg caagaaccgt acttcacctg gccgctgatt 4500
gctgctgacg ggggttatgc gttcaagtat gaaaacggca agtacgacat taaagacgtg 4560
ggcgtggata acgctggcgc gaaagcgggt ctgaccttcc tggttgacct gattaaaaac 4620
aaacacatga atgcagacac cgattactcc atcgcagaag ctgcctttaa taaaggcgaa 4680
acagcgatga ccatcaacgg cccgtgggca tggtccaaca tcgacaccag caaagtgaat 4740
tatggtgtaa cggtactgcc gaccttcaag ggtcaaccat ccaaaccgtt cgttggcgtg 4800
ctgagcgcag gtattaacgc cgccagtccg aacaaagagc tggcaaaaga gttcctcgaa 4860
aactatctgc tgactgatga aggtctggaa gcggttaata aagacaaacc gctgggtgcc 4920
gtagcgctga agtcttacga ggaagagttg gcgaaagatc cacgtattgc cgccaccatg 4980
gaaaacgccc agaaaggtga aatcatgccg aacatcccgc agatgtccgc tttctggtat 5040
gccgtgcgta ctgcggtgat caacgccgcc agcggtcgtc agactgtcga tgaagccctg 5100
aaagacgcgc agactaattc gagctcgaac aacaacaaca ataacaataa caacaacctc 5160
gggatcgagg gaaggatttc agaattcgcg gaagcgggta tcaccggcac gtggtacaac 5220
cagcatggtt ctaccttcac cgttaccgcg ggtgcggacg gtaacctgac cggtcagtac 5280
gaaaaccgtg cgcagggcac tggttgccag aactctccgt acaccctgac cggtcgttac 5340
aacggtacca aactggaatg gcgtgttgaa tggaacaact ctaccgaaaa ctgccactct 5400
cgtaccgaat ggcgtggtca gtaccagggt ggtgcggaag cgcgtatcaa cacccagtgg 5460
aacctgacct acgaaggtgg ttctggtccg gcgaccgaac agggtcagga caccttcacc 5520
aaagttaaac accaccacca ccaccactaa gtcgacctgc aggaagcttg tttaaacggt 5580
ctccagcttg gctgttttg 5599
<210> 66
<211> 26
<212> DNA
<213> Artificial Sequence
<220>
<223> regulatory gene
<400> 66
tctagagaaa gaggcgagtg tactag 26
<210> 67
<211> 26
<212> DNA
<213> Artificial Sequence
<220>
<223> regulatory gene
<400> 67
tctagagaaa gataggaggt tactag 26
<210> 68
<211> 26
<212> DNA
<213> Artificial Sequence
<220>
<223> regulatory gene
<400> 68
tctagagaaa gaggggacac tactag 26
<210> 69
<211> 26
<212> DNA
<213> Artificial Sequence
<220>
<223> regulatory gene
<400> 69
tctagagaaa gagcggaaac tactag 26
<210> 70
<211> 27
<212> DNA
<213> Artificial Sequence
<220>
<223> regulatory gene
<400> 70
ttctagagaa agatttgaat atactag 27
<210> 71
<211> 26
<212> DNA
<213> Artificial Sequence
<220>
<223> regulatory gene
<400> 71
tctagagaaa gaacggacat tactag 26
<210> 72
<211> 26
<212> DNA
<213> Artificial Sequence
<220>
<223> regulatory gene
<400> 72
tctagagaaa gacatgacta tactag 26
<210> 73
<211> 26
<212> DNA
<213> Artificial Sequence
<220>
<223> regulatory gene
<400> 73
tctagagaaa gaactgaaga tactag 26
<210> 74
<211> 26
<212> DNA
<213> Artificial Sequence
<220>
<223> regulatory gene
<400> 74
tctagagaaa gaggcgatcc tactag 26
<210> 75
<211> 26
<212> DNA
<213> Artificial Sequence
<220>
<223> regulatory gene
<400> 75
tctagagaaa gaagagagcc tactag 26
<210> 76
<211> 26
<212> DNA
<213> Artificial Sequence
<220>
<223> regulatory gene
<400> 76
tctagagaaa gacttgaggc tactag 26
<210> 77
<211> 27
<212> DNA
<213> Artificial Sequence
<220>
<223> regulatory gene
<400> 77
gaaccctaat acattaggag atcttct 27
<210> 78
<211> 27
<212> DNA
<213> Artificial Sequence
<220>
<223> regulatory gene
<400> 78
gaaccctaat acattaggac atattct 27
<210> 79
<211> 27
<212> DNA
<213> Artificial Sequence
<220>
<223> regulatory gene
<400> 79
gaaccctaat acattaggac atcatct 27
<210> 80
<211> 27
<212> DNA
<213> Artificial Sequence
<220>
<223> regulatory gene
<400> 80
gaaccctaat acataaggag atcatat 27
<210> 81
<211> 27
<212> DNA
<213> Artificial Sequence
<220>
<223> regulatory gene
<400> 81
gaaccctaat acataaggac ataatat 27
<210> 82
<211> 27
<212> DNA
<213> Artificial Sequence
<220>
<223> regulatory gene
<400> 82
gaaccctaat acataaggag attatct 27
<210> 83
<211> 27
<212> DNA
<213> Artificial Sequence
<220>
<223> regulatory gene
<400> 83
gaaccctaat acattaggag attatat 27
<210> 84
<211> 27
<212> DNA
<213> Artificial Sequence
<220>
<223> regulatory gene
<400> 84
gaaccctaat acataaggac atcttat 27
<210> 85
<211> 27
<212> DNA
<213> Artificial Sequence
<220>
<223> regulatory gene
<400> 85
gaacactaat acattaggag atcttct 27
<210> 86
<211> 27
<212> DNA
<213> Artificial Sequence
<220>
<223> regulatory gene
<400> 86
gaacactaat acataaggac ataatat 27
<210> 87
<211> 32
<212> DNA
<213> Artificial Sequence
<220>
<223> regulatory gene
<400> 87
ttaagtagtt aaacagggta tataggggaa ga 32
<210> 88
<211> 32
<212> DNA
<213> Artificial Sequence
<220>
<223> regulatory gene
<400> 88
ttaagtagtt aaacagggta tataggacga ga 32
<210> 89
<211> 32
<212> DNA
<213> Artificial Sequence
<220>
<223> regulatory gene
<400> 89
ttaagtagtt aaacagggta tatagggcta ta 32
<210> 90
<211> 32
<212> DNA
<213> Artificial Sequence
<220>
<223> regulatory gene
<400> 90
ttaagtagtt aaacagggta tataggagga ta 32
<210> 91
<211> 32
<212> DNA
<213> Artificial Sequence
<220>
<223> regulatory gene
<400> 91
ttaagtagtt aaacagggta tatagggcga ta 32
<210> 92
<211> 32
<212> DNA
<213> Artificial Sequence
<220>
<223> regulatory gene
<400> 92
ttaagtaatt aaacagggta tataggggaa ga 32
Claims (35)
- 모노머형 스트렙타비딘(monomeric streptavidin; mSA)을 코딩하는 유전자가 도입되어 형질 전환된 숙주 세포를 포함하는, 암을 진단하기 위한 조성물.
- 제 1항에 있어서,
상기 숙주 세포는 말토오즈 결합 단백질(maltose-binding protein; MBP)을 코딩하는 유전자가 더 도입된 것인, 조성물. - 제 1항에 있어서,
상기 숙주 세포는 상기 모노머형 스트렙타비딘을 코딩하는 유전자의 발현을 조절하는 조절 유전자가 더 도입된 것인, 조성물. - 제 3항에 있어서,
상기 조절 유전자는 리보솜 결합 부위(Ribosome binding site; RBS), 5'-비 번역 영역(5'-Untransrated Region; 5'-UTR) 및 전사 인자 결합 부위(transcription factor binding site)로 이루어진 군으로부터 선택되는 적어도 하나인 것인, 조성물. - 제 3항에 있어서,
상기 조절 유전자를 포함하는 재조합 벡터가 상기 숙주 세포에 형질 전환되는 경우, 상기 숙주 세포의 주변 세포질로 모노머형 스트렙타비딘이 발현되도록 하는 것인, 조성물. - 제 3항에 있어서,
상기 조절 유전자는 총 깁스 자유 에너지 변화량(ΔGtotal)이 0 이하인 것인, 조성물. - 제 3항에 있어서,
상기 조절 유전자는 번역 개시 속도(Translation Initiation Rate; TIR)가 특정 범위를 갖도록 조절된 것인, 조성물. - 제 3항에 있어서,
상기 조절 유전자의 서열 길이는 15 내지 39bp인 것인, 조성물. - 제 3항에 있어서,
상기 조절 유전자는 서열번호 5 내지 7 중 어느 하나로 표시되는 유전자 서열을 포함하는 것인, 조성물. - 제 9항에 있어서,
상기 조절 유전자에서, 상기 서열번호 5 내지 7 중 어느 하나로 표시되는 유전자 서열의 3' 말단에서 상기 모노머형 스트렙타비딘을 코딩하는 유전자의 개시코돈 까지의 간격이 6 내지 13bp인, 조성물. - 제 1항에 있어서,
상기 숙주 세포는 박테리아, 포유 동물, 식물 및 곤충으로 이루어진 군으로부터 선택되는 어느 하나 이상의 세포인 것인, 조성물. - 제 11항에 있어서,
상기 박테리아는 대장균, 스트렙토미세스 또는 살모넬라 속 균주인, 조성물. - 제 11항에 있어서,
상기 박테리아는 혐기성 균주인 것인, 조성물. - 제 11항에 있어서,
상기 박테리아는 약독화된 것인, 조성물. - 제 14항에 있어서,
상기 박테리아는 aroA, aroC, aroD, aroE, Rpur, htrA, ompR, ompF, ompC, galE, cya, crp, cyp, phoP, phoQ, rfaY, dksA, hupA, sipC, clpB, clpP, clpX, pab, nadA, pncB, pmi, rpsL, hemA, rfc, poxA, galU, cdt, pur, ssa, guaA, guaB, fliD, flgK, flgL, relA, spoA 및 spoT로 이루어진 군으로부터 선택되는 적어도 하나를 코딩하는 유전자가 변형된 것인, 조성물. - 제 14항에 있어서,
상기 박테리아는 구아노신-5-이인산-3-이인산(ppGpp)의 합성능이 결여된 것인, 조성물. - 제 1항에 있어서,
상기 암은 흑색종, 나팔관암, 뇌암, 소장암, 식도암, 임파선암, 담낭암, 혈액암, 갑상선암, 내분비선암, 구강암, 간암, 담도암, 대장암, 직장암, 자궁경부암, 난소암, 신장암, 위암, 십이지장암, 전립선암, 유방암, 뇌종양, 폐암, 갑상선 미분화암, 자궁암, 결장암, 방광암, 요관암, 췌장암, 뼈/연부조직 육종, 피부암, 비호지킨 림프종, 호지킨 림프종, 다발성 골수종, 백혈병, 골수이형성증후군, 급성 림프모구성 백혈병, 급성 골수성 백혈병, 만성 림프구성 백혈병, 만성 골수성 백혈병 및 고립성 골수종으로 구성되는 군으로부터 선택되는 어느 하나 이상인 것인, 조성물. - 제 1항에 있어서,
상기 조성물은 비오틴화된 화합물을 더 포함하는 것인, 조성물. - 제 18항에 있어서,
상기 화합물은 조영제인 것인, 조성물. - 제 19항에 있어서,
상기 조영제는 방사성 핵종, 형광 표지, 효소 표지, 화학 발광 마커, 금 제제 및 자성 제제로 이루어진 군으로부터 선택되는 적어도 하나인 것인, 조성물. - 모노머형 스트렙타비딘을 발현하는 숙주 세포가 투여된 목적하는 개체에서, 상기 숙주 세포를 촬영 수단으로 확인하는 단계를 포함하는, 암의 진단을 위한 정보 제공 방법.
- 제 21항에 있어서,
상기 목적하는 개체는 비오틴화된 화합물이 더 투여된 것인, 정보 제공 방법. - 제 21항에 있어서,
상기 확인하는 단계는 복수 회에 걸쳐 수행되는, 정보 제공 방법. - 모노머형 스트렙타비딘(monomeric streptavidin; mSA)을 코딩하는 유전자가 도입되어 형질 전환된 숙주 세포를 포함하는, 암을 예방 또는 치료하기 위한 약학적 조성물.
- 제 24항에 있어서,
상기 숙주 세포는 말토오즈 결합 단백질(maltose-binding protein; MBP)을 코딩하는 유전자가 더 도입된 것인, 조성물. - 제 24항에 있어서,
상기 숙주 세포는 상기 모노머형 스트렙타비딘을 코딩하는 유전자의 발현을 조절하는 조절 유전자가 더 도입된 것인, 조성물. - 제 26항에 있어서,
상기 조절 유전자는 리보솜 결합 부위(Ribosome binding site; RBS), 5'-비 번역 영역(5'-Untransrated Region; 5'-UTR) 및 전사 인자 결합 부위(transcription factor binding site)로 이루어진 군으로부터 선택되는 적어도 하나인 것인, 조성물. - 제 26항에 있어서,
상기 조절 유전자를 포함하는 재조합 벡터가 상기 숙주 세포에 형질 전환되는 경우, 상기 숙주 세포의 주변 세포질로 모노머형 스트렙타비딘이 발현되도록 하는 것인, 조성물. - 제 26항에 있어서,
상기 조절 유전자는 총 깁스 자유 에너지 변화량(ΔGtotal)이 0 이하인 것인, 조성물. - 제 26항에 있어서,
상기 조절 유전자는 번역 개시 속도(Translation Initiation Rate; TIR)가 특정 범위를 갖도록 조절된 것인, 조성물. - 제 26항에 있어서,
상기 조절 유전자의 서열 길이는 15 내지 39bp인 것인, 조성물. - 제 26항에 있어서,
상기 숙주 세포는 박테리아, 포유 동물, 식물 및 곤충으로 이루어진 군으로부터 선택되는 어느 하나 이상의 세포인 것인, 조성물. - 제 24항에 있어서,
상기 암은 흑색종, 나팔관암, 뇌암, 소장암, 식도암, 임파선암, 담낭암, 혈액암, 갑상선암, 내분비선암, 구강암, 간암, 담도암, 대장암, 직장암, 자궁경부암, 난소암, 신장암, 위암, 십이지장암, 전립선암, 유방암, 뇌종양, 폐암, 갑상선 미분화암, 자궁암, 결장암, 방광암, 요관암, 췌장암, 뼈/연부조직 육종, 피부암, 비호지킨 림프종, 호지킨 림프종, 다발성 골수종, 백혈병, 골수이형성증후군, 급성 림프모구성 백혈병, 급성 골수성 백혈병, 만성 림프구성 백혈병, 만성 골수성 백혈병 및 고립성 골수종으로 구성되는 군으로부터 선택되는 어느 하나 이상인 것인, 조성물. - 제 24항에 있어서,
상기 조성물은 비오틴화된 화합물을 더 포함하는 것인, 조성물. - 제 34항에 있어서,
상기 비오틴화된 화합물은 비오틴화된 암 치료 물질인, 조성물.
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