KR20220056515A - NANO EMULSION COMPOSITION COMPRISING Squalene, COSMETIC COMPOSITION COMPRISING THE SAME, AND METHOD FOR PREPARING SAME - Google Patents

Abstract

The present invention provides a nanoemulsion composition comprising more than 20 wt% of squalene based on the total weight of the entire oily phase, a cosmetic composition comprising the same, and a preparation method thereof. The nanoemulsion composition scavenges free radicals, increases the expression of type I procollagen protein, inhibits the expression of MMP-1 protein, and has the ability to inhibit elastase activity to exhibit effects such as skin whitening, skin elasticity promotion, wrinkle prevention, wrinkle improvement, and skin moisturizing.

Description

Nanoemulsion composition containing squalene, cosmetic composition containing same, and manufacturing method thereof

The present invention relates to a nanoemulsion composition comprising squalene, a cosmetic composition comprising the same, and a method for preparing the same, and more particularly, the present invention improves the stability of squalene, and at the same time, free radical scavenging ability, cell viability, elastase Nanoemulsion composition excellent in inhibitory ability, MMP-1 inhibitory ability and collagenase inhibitory ability to have excellent skin whitening, skin elasticity enhancement, wrinkle prevention, wrinkle improvement and skin moisturizing effect, and a cosmetic composition comprising the same and a method for manufacturing the same will be.

Squalene is a triterpenoid-based unsaturated hydrocarbon generally obtained from shark liver, and it can be said to be an intermediate in the biosynthesis process of steroids, hopanoids, and triterpenes. .

Squalene was first isolated from shark liver oil in 1916. However, squalene is also present in seeds and oils of plants and freshwater fish, and it has been reported that humans produce about 1 g per day. In general, squalene has various functions and is used in food, cosmetics, health food, and pharmaceutical industries.

Not only sharks but also palm fruits and olives contain squalene. Generally, olives contain 400 to 450 mg/100 g of squalene, and high-quality olives have 700 mg/100 g.

In the past, shark liver oil and vegetable oil were the main sources of squalene, but recently, squalene is produced using a microbial fermentation process.

The IUPAC names of squalene are 2, 6, 10, 15, 19, 23-hexamethyltetracosa-2, 6, 10, 14, 18, 22-hexaene. It is made up of molecules of 30 carbons and 50 hydrogens.

Squalene has a low freezing point (-60℃) compared to its molecular weight, and is a precision machine oil with immobility. It is used as a stationary phase liquid for gas chromatography and as a cosmetic raw material. In particular, squalene in the cosmetic industry accounts for about 13% as a component of skin surface lipids, has excellent skin affinity, and is one of the natural substances that is deeply absorbed without any residue when applied.

In addition, it is a highly unsaturated hydrocarbon with 6 double bonds and has strong reducing power, so it binds with active oxygen and prevents oxidation and damage to cells, contributing to health maintenance and promotion. As a precursor very important in the biosynthesis of cholesterol, it has the ability to heal wounds, antioxidant effect, and reduce wrinkles, a phenomenon of skin aging by increasing collagen.

As we enter an aging society, the anti-aging (anti-aging) trend is rapidly spreading and the demand for related products is rapidly increasing, so research is being actively conducted to effectively use squalene for various purposes, which has an excellent anti-aging effect. .

In particular, since squalene has low solubility and stability, there are many restrictions in the manufacture of various products. Therefore, in addition to the improvement of the solubility of squalene, the stability of squalene involved in discoloration and discoloration due to air, moisture, and light is also required.

Korean Patent Registration No. 10-1884083 (Registration Date: 2018.07.25)

An object of the present invention is to improve the solubility and stability of squalene in the form of a nano-emulsion, minimize changes over time such as color, formulation stability, and odor due to temperature change, light, air, etc. An object of the present invention is to provide a nanoemulsion composition having excellent effects such as skin whitening, skin elasticity enhancement, wrinkle prevention, wrinkle improvement and skin moisturizing that can be used, and a cosmetic composition comprising the same.

Another object of the present invention is to provide an effective method for preparing such a nano-emulsion composition and a cosmetic composition comprising the same.

An embodiment of the present invention may provide a nano-emulsion composition containing 10 to 40% by weight of squalene based on the total weight of the total oil phase.

On the other hand, the nano-emulsion composition further comprises a vegetable nonionic surfactant, the vegetable nonionic surfactant is any one of polyglyceryl-10 oleate (polyglyxeryl-10 oleate) and sucrose distearate (sucrose distearate) may include more than one.

In addition, the nano-emulsion composition may include any one or more of cetylethyl hexanoate and tocopheryl acetate.

The squalene may include fermented squalene.

The nano-emulsion composition may have a specific gravity of 0.8 to 1,1, a pH of 4.5 to 8.5, and an average particle size of 50 to 300 nm.

On the other hand, the nano-emulsion composition may have the ability to scavenge free radicals, increase the expression of type I procollagen protein, suppress the expression of MMP-1 protein, and inhibit elastase activity.

The nano-emulsion composition may have an effect for preventing or improving skin wrinkles.

According to another preferred embodiment of the present invention, a first step of dissolving a vegetable nonionic surfactant and an emollient;

A second step of stirring by adding a stabilizer;

a third step of adding squalene and cooling; and

A fourth step of preparing a nano-emulsion with a homogenizer,

The vegetable nonionic surfactant is at least one of polyglyceryl-10 oleate and sucrose distearate,

A method for preparing a nano-emulsion composition may be provided.

According to another preferred embodiment of the present invention, it is possible to provide a cosmetic composition comprising the nano-emulsion composition.

According to another preferred embodiment of the present invention, cosmetics including the cosmetic composition may be provided, and the cosmetics include skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutritional lotion, Massage cream, nourishing cream, moisture cream, hand cream, foundation, essence, nourishing essence, ampoule, pack, soap, cleansing foam, cleansing lotion, cleansing cream, BB cream, CC cream, lip cream, lipstick, sea lotion, sea cleanser, It may be any one selected from the group of eye creams, foundations, and cosmetics in which one or more of them are combined.

The nanoemulsion composition of the present invention and a cosmetic composition comprising the same can improve the solubility and stability of squalene at the same time.

Through this, it is possible to stably provide squalene, which is easily deteriorated due to changes over time, such as color, formulation stability, reshaping, and odor, to the skin tissue in daily environments such as air, temperature change, light, and moisture.

In addition, the nano-emulsion composition scavenges free radicals, increases the expression of type I procollagen protein, suppresses the expression of MMP-1 protein, and has the ability to inhibit elastase activity, so that skin whitening, skin elasticity It shows effects such as enhancement, wrinkle prevention, wrinkle improvement and skin moisturizing.

1 is a photograph of the nano-emulsion (A), 0.1% diluted aqueous solution (B) and 1% diluted aqueous solution (C) of Examples.
2 is a particle size analysis result of measuring the particle size of the 0.1% diluted aqueous solution (B) and 1% diluted aqueous solution (C) of the nano-emulsion of Example.
3 is a graph showing the measurement results of DPPH free radical scavenging activity of squalene (SQ), fermented squalene (F-SQ), and squalene nano emulsion (MF-SQ).
Figure 4 is a graph showing the long-term stability measurement results of squalene nano-emulsion (MF-SQ) and MF-SQ applied formulation.

Accordingly, the present inventors have studied to solve the above problems, and as a result, a stabilized nano-emulsion composition formed by adding squalene in excess was prepared.

By nano-emulsifying excess squalene to increase solubility and stability, suppress odor caused by changes in external environment, and prepare a nano-emulsion composition with excellent effects such as skin whitening, skin elasticity enhancement, wrinkle prevention, wrinkle improvement and skin moisturizing The present invention has been completed. The present invention will be described in more detail as follows.

The nano-emulsion composition of the present invention may contain 10 to 40% by weight of squalene based on the total weight of the total oil phase.

On the other hand, the nano-emulsion composition further comprises a vegetable nonionic surfactant, the vegetable nonionic surfactant is any one of polyglyceryl-10 oleate (polyglyxeryl-10 oleate) and sucrose distearate (sucrose distearate) may include more than one.

Preferably, polyglyceryl-10 oleate (polyglyxeryl-10 oleate) and sucrose distearate may be used together, and the content of the vegetable nonionic surfactant is at least 10% by weight based on the total weight of the total oil phase. , at most 50% by weight or less. It may be 40 wt% or more and 50 wt% or less.

The content ratio of the polyglyceryl-10 oleate and sucrose distearate may be 2:1. More specifically, the most stable nanoemulsion is produced when polyglyceryl-10 oleate is 28 wt% and sucrose distearate is 14 wt%.

The vegetable nonionic surfactant is a plant-derived nonionic surfactant, which is safe for the human body and biodegradable compared to chemically synthesized surfactants, thereby helping to protect the environment.

Polyglyceryl-10 oleate is a vegetable nonionic surfactant produced by ester-bonding glycerin with oleic acid, an unsaturated fatty acid contained in a large amount in olive oil. It is a light brown viscous liquid. Polyglyceryl-10 oleate can maintain the moisture content of the skin for a long time.

In addition, sucrose distearate is a vegetable nonionic surfactant prepared by ester-bonding stearic acid and sucrose, usually obtained by hydrolyzing coconut or palm oil.

When polyglyceryl-10 oleate and sucrose distearate are properly mixed and used, when dispersed in water, a self-organized body in the form of a multi-layer lamellar can be formed. This lamellar form is similar to the lamellar structure of the stratum corneum, which is the outermost layer of the skin, and thus increases affinity with the skin to enhance skin permeability.

In addition, the nano-emulsion composition may include any one or more of cetylethyl hexanoate and tocopheryl acetate. Specifically, 10 to 40% by weight of cetylethyl hexanoate and 0.05 to 0.3% by weight of tocopheryl acetate may be used as a mixture. Here, 10 to 40% by weight of squalene may be mixed, and the total amount thereof may be 30 to 75% by weight of the composition.

Preferably, it may include both cetylethylhexanoate and tocopheryl acetate.

Cetylethylhexanoate may be included in an amount of 20 wt% or more and 50 wt% or less based on the total weight of the total oil phase as a softening agent. Cetylethylhexanoate is prepared by esterifying cetyl alcohol and 2-ethylhexanoic acid, spreads well without stickiness and has a low coagulation point. It is a colorless-pale yellow transparent liquid that helps the skin to absorb functional ingredients, softens the skin texture, protects the skin, and prevents moisture loss. The cetylethylhexanoate can be used as an emollient (or softening agent), and instead of this raw material, ethylhexyl palmitate, ester-type oils of capric/caprylic triglyceride, and hydrogenated polydecene are used as cosmetic raw materials. The hydrocarbon oils used may be used alone or in combination.

In addition, tocopheryl acetate may be included as a stabilizer in an amount of 0.05 wt% or more and 0.3 wt% or less based on the total weight of the total oil phase.

Tocopheryl acetate is produced through the acetylation of tocopherol (vitamin E) and acetic acid, a fat-soluble vitamin, and is widely used as an alternative to pure tocopherol.

It is an antioxidant component that inhibits the oxidation of unsaturated fatty acids constituting cell membranes, and is known to have the effect of delaying and preventing skin aging and various pathological phenomena. In addition, the synergistic effect and skin moisturizing effect when used in combination with vitamin C are also known.

In addition, water may be included in an amount of 0.5 wt% to 7 wt% or less based on the total weight of the total nanoemulsion composition when preparing the entire nanoemulsion. The prepared nano-emulsion can be uniformly dispersed in various media including water by controlling the concentration.

In addition, the squalene of the nano-emulsion composition may include fermented squalene. Fermented squalene is squalene obtained using the fermentation process of microorganisms.

Meanwhile, the specific gravity of the nano-emulsion composition may be 0.8 to 1.1, the pH may be 4.5 to 8.5, and the average particle size may be 50 to 300 nm.

The specific gravity, pH, etc. of the nano-emulsion composition are suitable for use as a cosmetic.

It is possible to scavenge free radicals of the nanoemulsion composition, increase the expression of type I procollagen protein, and inhibit the expression of MMP-1 protein.

Type I procollagen is the main material for synthesizing type I collagen, which constitutes most of the extracellular matrix, and the increase in procollagen affects wrinkle improvement by participating in the skin's bonding strength and elasticity.

In addition, MMP is an enzyme involved in the reduction of extracellular matrix (ECM) proteins such as elastin and collagen as zinc-dependent endopeptidase, expression is regulated. Among them, MMP-1 plays an important role in photoaging, and affects skin aging by decomposing collagen types 1, 2, 3, and 5.

Therefore, the nano-emulsion composition can exhibit the effect of improving skin aging by inhibiting the expression of MMP-1 protein.

In addition, the nano-emulsion composition may inhibit the activity of elastase.

Elastase is an enzyme that decomposes elastin, an elastic fiber that is abundantly present in the extracellular matrix, plays an important role in skin elasticity and occupies 3-4% of the dermal layer.

The nano-emulsion composition may inhibit the activity of elastase, increase skin elasticity, and inhibit skin wrinkle formation.

According to another preferred embodiment of the present invention, the first step of dissolving the vegetable nonionic surfactant and the softening agent;

A second step of stirring by adding a stabilizer;

a third step of adding squalene and cooling; and

A fourth step of preparing a nano-emulsion with a homogenizer,

The vegetable nonionic surfactant is at least one of polyglyceryl-10 oleate and sucrose distearate,

A method for preparing a nano-emulsion composition may be provided.

In the first step, the reaction may proceed at a temperature of 50 to 80° C., and cetyl ethyl hexanoate may be used as a softening agent.

In the second step, the reaction may be performed at a temperature of 30 to 50° C., and tocopheryl acetate may be used as a stabilizer.

In addition, the cooling temperature of the third step may be 20 ~ 40 ℃, the squalene may be fermentation squalene.

The homogenizer of the fourth step may be a high-pressure injection homogenizer, and may react under a reaction pressure and reaction temperature of 1,000 to 50,000 psi, 10 to 40°C.

According to another preferred embodiment of the present invention, it is possible to provide a cosmetic composition comprising the nano-emulsion composition.

According to another preferred embodiment of the present invention, cosmetics including the cosmetic composition can be provided, and the cosmetics are skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutritional lotion, massage. Cream, Nourishing Cream, Moisture Cream, Hand Cream, Foundation, Essence, Nourishing Essence, Ampoule, Pack, Soap, Cleansing Foam, Cleansing Lotion, Cleansing Cream, BB Cream, CC Cream, Lip Cream, Lipstick, Sea Lotion, Sea Cleanser, Eye It can be applied in various ways to cosmetics of all formulations that have been devised or can be devised in the future, such as creams, foundations, and cosmetics in which one or more thereof is combined.

Hereinafter, an embodiment of the present invention will be described in more detail through the following examples and experimental examples. However, the present invention is not limited to the following examples and experimental examples.

[Example] Preparation method of squalene nano emulsion

A method for preparing a squalene nanoemulsion was prepared using the composition table shown in Table 1 below. The surfactant used here is MIZOAN CleanEmul-7300, a raw material in which polyglyceryl-10 oleate and sucrose distearate are mixed.

The nano-emulsion containing squalene was prepared according to the following procedure with the components and compositions of Table 1 below.

1) Phase A and phase B were weighed and dissolved by heating at 60~70℃.

2) After cooling to 45°C, phase C was added and stirred. Since it is weak to heat, it was added at the lowest possible temperature (40~45℃).

3) Phase D was added, mixed, and cooled to 30°C.

4) It was prepared by passing through a high pressure homogenizer at 10,000psi, 20~30℃, and 3~5 times through a nano-cell.

5) When it becomes a transparent nano-emulsion, the preparation is completed. In order to prevent an increase in the friction temperature due to passage with high pressure, it was applied in the manufacture using equipment equipped with a cooling circulation.

ingredient content
(wt%) note (A) MIZOAN CleanEmul-7300 42.000 Surfactants
(Surfactant) (B) cetylethyl hexanoate 35.900 Emollient (C) tocopheryl acetate 0.100 stabilizer
(Stabilizer) (D) Fermented Squalene 20.000 active substance
(active) (E) D. I. Water 2.000 menstruum
(solvent) entire 100.000

The general properties of the nano-emulsion encapsulated in fermented squalene prepared according to the above example were measured and shown in Table 2 below.

The appearance of the prepared squalene nanoemulsion was a clear, transparent, colorless liquid that had a unique odor of squalene, and had a specific gravity of 0.928 and a pH of 5.82.

It was confirmed that excessive squalene was stably encapsulated, heavy metals were not detected, and the range of microorganisms was found to be suitable for cosmetic standards.

measurement result Exterior Transparency smell squalene odor colour colorless importance
(Sepecific Gravity) 0.928 pH 5.82 Squalene content 20.05 heavy metal content Pb, As not detected microbe 1000 CFU/mL or less

The final product of the prepared squalene nano-emulsion and the sample obtained by aqueous solution (squalene nano-emulsion diluted in water) are shown in FIG. 1 . The final product of the squalene nano-emulsion (A), the aqueous solution (B) in which the squalene nano-emulsion is diluted to 0.1% concentration, and the aqueous solution (C) in which the squalene nano-emulsion is diluted to a concentration of 1% are shown, respectively.

As shown in Figure 1, the squalene nanoemulsion exists in a transparent and stable state (A), and when it is diluted by putting it in purified water, it has a color because it is inverted into fine emulsified micelles (B and C).

Apparently, the aqueous solution (B) in which the squalene nano-emulsion is diluted to 0.1% concentration is bluer than the aqueous solution (C) in which the squalene nano-emulsion is diluted to 1% concentration. do.

The results of measuring the particle size distribution with 0.1% and 1% dilution solutions of the squalene nanoemulsion are shown in FIG. 2 . Figure 2a is a sample diluted to a concentration of 0.1%, Figure 2b is a measurement result of a sample diluted to a concentration of 1% with a particle meter.

The average particle size distribution of the 0.1% diluted solution of squalene nanoemulsion was 134.8 nm, the standard deviation was 33.60, and the average particle size distribution of the 1% diluted solution was 131.2 nm, and the standard deviation was 39.70.

The graph of the particle size distribution appeared as a narrow peak, confirming that a squalene nanoemulsion having a uniform particle size was formed.

[Experimental Example 1] Measurement of antioxidant properties

The radical scavenging ability of 2,2-diphenyl-1-picrylhydrazyl (DPPH), a standard reagent used for antioxidant measurement, was measured according to the method of Blois, through which The antioxidant capacity between samples was evaluated. After adding 0.2 mM DPPH solution and the sample to a 96 well plate and reacting in the dark for 30 minutes, absorbance was measured at 517 nm using an ELISA reader. As a positive control, L-ascorbic acid (L-AA) was used. The evaluation formula is shown in the following formula (1).

Inhibition rate (%) = (1 - Absorbance of the experimental group / Absorbance of the control group) × 100 (1)

As shown in Equation (1), antioxidant capacity was calculated through absorbance by subtracting the value obtained by dividing the absorbance of the control group by the absorbance of the experimental group in order to comply with the Beer Lambert law as shown in Equation (1).

As shown in FIG. 3 , the DPPH radical scavenging ability of squalene (SQ), fermented squalene (F-SQ), squalene nanoemulsion (MF-SQ) and L-ascorbic acid was measured, through which they Eggplant had antioxidant ability.

SQ was compared at the highest soluble concentration because it was impossible to compare at the same concentration with other samples due to poor solubility. As a result, F-SQ and MF-SQ showed high antioxidant activity at 250 ppm, respectively, at 80.72%, 81.5%, and 93.05% of L-ascorbic acid.

That is, it was possible to confirm the high antioxidant activity of MF-SQ, which eliminated the disadvantages of SQ with low solubility.

[Experimental Example 2] Cytotoxicity measurement

Cell lines and cell culture methods used for cytotoxicity measurement are as follows.

Neonatal Human Dermal Fibroblasts (NHDF-Neo) were purchased from Lonza (Morrisville, NC, USA) and added with 10% fetal bovine serum (FBS) (Gibco, USA) and 1% penicillin-streptomycin (Sigma, USA). Dulbecco's modification of eagle's medium (DMEM) (Corning, USA) was used as a medium and cultured at 37°C and 5% CO ₂ conditions, and a serum-free medium except for FBS was used for sample processing.

For cytotoxicity measurement, NHDF-Neo cells were dispensed in a 96-well plate to 1×10⁴cells/well and cultured for 24 h. , 2.5, 5, 10 ppm). After 24 h of incubation, 20 μL of MTT solution (5 mg/mL in PBS) was added to each well, followed by reaction at 37° C. and 5% CO ₂ condition for 3 h. After completion of the reaction, the supernatant was sucked and DMSO was dispensed to dissolve insoluble formazan. Then, the absorbance was measured at 570 nm using an ELISA reader, and the cell viability (%) is shown in Equation (2) below.

Survival rate (%) = (absorbance of the experimental group / absorbance of the control group) × 100% (2)

When NHDF-Neo cells were treated with samples of SQ and MF-SQ by concentration (2.5, 5, 10 ppm), no toxicity was observed in all sections.

In addition, SQ and MF-SQ showed a cell viability of 121.2% and 129.9%, respectively, at a concentration of 10 ppm, confirming that the cell viability was higher than that of L-ascorbic acid, which showed a cell viability of 117.5%.

That is, it was confirmed that cell viability could be improved by nano-emulsifying squalene.

[Experimental Example 3] Elastase (Elastase) activity measurement

NHDF-Neo cells were used to measure the elastase activity of squalene (SQ) and squalene nanoemulsion (MF-SQ). Cells were dispensed and cultured in a 60 mm Petri dish to obtain the required number of cells. After removing the medium and washing with DPBS (Dulbecco's phosphate-buffered saline), 0.2 M Tris-HCl buffer (pH 8.0) containing 0.1% triton X-100 was added to extract the protein.

The supernatant obtained by centrifuging this solution at 3,000 rpm at 4°C for 20 minutes was used as an elastase enzyme solution, and the amount of protein was quantified using a BCA kit (Thermo, USA). Elastase enzyme solution was dispensed into 96 wells so that the amount of protein in each well was 100 μg, 0.2 M Tris-HCl buffer (pH 8.0), 50 mM STANA, and samples were added and reacted at 37° C. for 90 minutes. As a positive control, phosphoramidon was used, and after completion of the reaction, absorbance was measured at 405 nm using an ELISA reader. Elastase activity (%) was expressed by the following formula (3).

Elastase activity (%): (absorbance of the experimental group / absorbance of the control group) × 100 (3)

As a result of the measurement, when SQ and MF-SQ were reacted with elastase enzyme solution extracted from NHDF-Neo cells, both showed the same level of inhibition of 8.7% at a concentration of 10 ppm.

That is, it was confirmed that the elastase inhibitory ability could be maintained even when squalene was nano-emulsified.

[Experimental Example 4] Type I procollagen (Procollagen type I) C-peptide (C-peptide) biosynthesis measurement

In order to measure the amount of collagen biosynthesis in the cell culture medium, NHDF-Neo cells were aliquoted in a 12 well plate to 1×10 cells/well, cultured for 24 h, and then added after diluting the sample in a serum-free medium. After 24 h of reaction, the amount of collagen biosynthesis was measured with the supernatant obtained by collecting the culture solution from each well and centrifuging at 3,000 rpm for 5 min. Procollagen type I C-peptide EIA kit (TaKaRa, Japan) was used to measure the amount of collagen biosynthesis.

As a result of the measurement, the biosynthetic performance of type I procollagen of squalene (SQ) and squalene nanoemulsion (MF-SQ) was 5% and 3.5%, respectively, at 10 ppm concentration, indicating that both of them can contribute to a certain level of collagen production. Confirmed.

[Experimental Example 5] Matrix metalloproteinase-1 (MMP-1) production amount measurement

Human total MMP-1 Duoset (R&D Systems, USA) was used to measure the amount of MMP-1 produced in the cell culture medium.

First, NHDF-Neo cells were aliquoted to 1×10 cells/well in a 12 well plate and cultured for 24 h. Then, the medium was removed, washed with DPBS, and 30 mJ/cm ² UVB was applied to the plate with DPBS thin. investigated. After UVB irradiation, the sample diluted in serum-free medium and TNF-α (10 ng/mL) were simultaneously treated and reacted for 24 h.

At this time, TNF-α was added to give more stimulation to the NHDF-Neo cells. Then, the amount of MMP-1 production was measured using the supernatant obtained by collecting the culture solution from each well and centrifuging at 3,000 rpm for 5 min.

By measuring the degree of reduction in the expression of MMP-1 when SQ and MF-SQ were treated in NHDF-Neo cells damaged by external stimuli, the wrinkle improvement effect of the sample could be found.

When NHDF-Neo cells were irradiated with UVB (30 mJ/cm ² ) and treated with TNF-α (10 ng/mL), the amount of MMP-1 production increased by 34.4%, and squalene (SQ) and squalene nanoemulsion (MF-SQ) was reduced by 1.55% and 31.79%, respectively.

Through these results, it was confirmed that the amount of decrease in MMP-1 increased by external stimuli such as UVB could be greatly reduced by nano-emulsifying squalene.

[Experimental Example 6] Long-term stability measurement

Long-term stability (6 months) was confirmed through HPLC analysis of squalene in the squalene nanoemulsion (MF-SQ) and the essence formulation to which it was applied. It was confirmed that 70% of squalene remained at 25 weeks at 25 °C and 50 °C conditions, and 80% or more of MF-SQ was maintained at 26 weeks (FIG. 4a).

In addition, as shown in FIG. 4b , the formulation to which MF-SQ was applied was quantitatively analyzed at 4° C., 25° C., and 50° C., all of which were quantitatively analyzed after 12 weeks, confirming that MF-SQ greatly contributes to stabilizing squalene. could

Although described above with reference to the embodiments and experimental examples of the present invention, those of ordinary skill in the art to which the present invention pertains will be able to make various applications and modifications within the scope of the present invention based on the above content.

Claims (6)

Contains 10 to 40% by weight of squalene based on the total weight of the total oil phase,
Contains a vegetable nonionic surfactant,
Containing any one or more of cetylethyl hexanoate (cetylethyl hexanoate) and tocopheryl acetate (tocopheryl acetate),
The vegetable nonionic surfactant comprises any one or more of polyglyceryl-10 oleate (polyglyxeryl-10 oleate) and sucrose distearate,
Nanoemulsion composition. According to claim 1,
The squalene comprises fermented squalene,
Nanoemulsion composition. According to claim 1,
The specific gravity is 0.8 to 1,1,
pH is 4.5-8.5,
with an average particle size of 50-300 nm,
Nanoemulsion composition. According to claim 1,
Scavenging free radicals, increasing the expression of type I procollagen protein, inhibiting the expression of MMP-1 protein, having the ability to inhibit elastase activity, and having an effect for preventing or improving skin wrinkles,
Nanoemulsion composition. a first step of dissolving a vegetable nonionic surfactant and an emollient;
A second step of stirring by adding a stabilizer;
a third step of adding squalene and cooling; and
A fourth step of preparing a nano-emulsion with a homogenizer,
The vegetable nonionic surfactant is at least one of polyglyceryl-10 oleate and sucrose distearate,
Method for preparing a nano-emulsion composition. In a cosmetic comprising a cosmetic composition comprising the nano-emulsion composition of any one of claims 1 to 4,
The cosmetics include skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nourishing lotion, massage cream, nourishing cream, moisture cream, hand cream, foundation, essence, nourishing essence, ampoule, pack, soap, Cosmetics that are selected from the group consisting of cleansing foam, cleansing lotion, cleansing cream, BB cream, CC cream, lip cream, lipstick, sea lotion, sea cleanser, eye cream, foundation, and combinations of one or more thereof.

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