KR20220052580A - Preparation method of psyllium powder fermented by lactic acid bacteria - Google Patents
Preparation method of psyllium powder fermented by lactic acid bacteria Download PDFInfo
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- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
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Abstract
Description
본 발명은 유산균 배양액을 이용하여 슈퍼푸드 등 일상생활에서 접할 수 있는 식재료를 유산균과 함께 발효시키고 이를 분말 형태로 제조하는 김치 유산균을 이용한 포스트 바이오틱스 제조 방법에 관한 것이다. The present invention relates to a method for producing post-biotics using kimchi lactic acid bacteria, in which food materials that can be encountered in daily life, such as superfoods, are fermented together with lactic acid bacteria and manufactured in powder form using a lactic acid bacteria culture medium.
포스트 바이오틱스는 유산균 배양 건조물을 말한다. 유산균이 만들어내는 유기산, 즉 박테리오신(Bacteriocin), 뷰틸레이트, 효소, 아미노산, 펩타이드 등 유산균 대사산물이 대표적이다.Post-biotics refer to lactic acid bacteria culture dried products. Organic acids produced by lactic acid bacteria, that is, lactic acid bacteria metabolites such as bacteriocin, butylate, enzymes, amino acids, and peptides, are representative.
포스트 바이오틱스는 ‘프로 바이오틱스’와 그의 먹이인 ‘프리 바이오틱스’, 이 둘을 함께 지칭하는 ‘신 바이오틱스’에 이어 4세대 유산균으로 주목받고 있다.Post-biotics are attracting attention as the fourth-generation lactic acid bacteria, following ‘probiotics’ and ‘prebiotics’ that are their food, and ‘new biotics’, which refer to both.
프로 바이오틱스는 유산균을 비롯해 몸속에서 유익한 효과를 내는 모든 균을 의미한다. 장뿐만 아니라 면역질환 등 다양한 신체 기능에 관여한다. 한동안 프로 바이오틱스의 먹이가 되는 식이섬유(프리 바이오틱스)를 함께 복용하면 효과가 더 좋다는 연구가 급물살을 탔는데, 최근에는 프로 바이오틱스가 내놓는 대사산물인 포스트 바이오틱스가 치료제나 질병 진단에 효율적이라는 주장이 주목받고 있다.Probiotics refer to all bacteria that have beneficial effects in the body, including lactic acid bacteria. It is involved in various bodily functions such as the intestine as well as immune diseases. For a while, research showing that taking together dietary fiber (prebiotics), which is the food of probiotics, has a better effect has been on the rise. The claim is receiving attention.
본 발명은 차전자피를 유산균 배양액과 혼합 및 발효시켜 마련되는 유산균 대사산물을 포스트 바이오틱스 분말 형태로 제공하기 위한 제조 방법을 제공하는데 있다. An object of the present invention is to provide a manufacturing method for providing lactic acid bacteria metabolites prepared by mixing and fermenting psyllium psyllium with a lactic acid bacteria culture medium in the form of post biotic powder.
본 발명의 차전자피 포스트 바이오틱스 제조방법은, 유산균을 액상배지에 접종하여 유산균 액상물을 포함하는 제1 액상물을 배양 하는 단계; 원물을 착즙기에 넣어 원물 착즙액을 만드는 단계; 차전자피를 분쇄기에 넣어 차전자피 분말을 준비하는 단계; 상기 제1 액상물, 상기 원물 착즙액 및 상기 차전자피 분말을 혼합하여 제2 액상물로 만드는 단계; 상기 제2 액상물을 배양하여 발효액을 만드는 단계; 상기 발효액을 건조하여 제3 액상물을 만드는 단계; 상기 제3 액상물을 열처리하고 분쇄하여 분말 형태의 차전자피 포스트 바이오틱스를 만드는 단계; 를 포함할 수 있다.The method for producing psyllium hull post-biotics of the present invention comprises the steps of inoculating lactic acid bacteria in a liquid medium and culturing a first liquid containing the lactic acid bacteria liquid; Putting the raw material into a juicer to make the raw material juice; preparing psyllium hull powder by putting the psyllium hull into a grinder; mixing the first liquid, the raw juice, and the psyllium hull powder to make a second liquid; culturing the second liquid to make a fermentation broth; drying the fermentation broth to make a third liquid; heat-treating and pulverizing the third liquid to produce psyllium hull post-biotics in powder form; may include
포스트 바이오틱스는 장 기능 개선을 통한 예방 건강과 건강 증진에 도움을 주기 때문에 세균성 장 질환자, 잦은 배탈, 비만, 당뇨, 다이어트가 필요한 사람 등에게 도움이 될 수 있다.Because post-biotics help to promote preventive health and health by improving intestinal function, it can be helpful for people with bacterial bowel disease, frequent stomach upsets, obesity, diabetes, and those who need a diet.
포스트 바이오틱스는 프로 바이오틱스가 먹이인 프리 바이오틱스를 먹고 만들어내는 대사 산물로 유기산, 박테리오신(Bacteriocin), EPS, 효소 등을 나타낸다.Postbiotics are metabolites produced by eating prebiotics, the food of which probiotics are fed, and represent organic acids, bacteriocins, EPS, and enzymes.
포스트 바이오틱스는 '프로 바이오틱스’와 그의 먹이인 ‘프리 바이오틱스’, 이 둘을 함께 지칭하는 ‘신 바이오틱스’에 이어 4세대 유산균으로 주목받고 있다.Post-biotics are attracting attention as the fourth-generation lactic acid bacteria following 'probiotics' and 'prebiotics', which are their food, and 'new biotics', which refer to both.
프로 바이오틱스는 유산균을 비롯해 몸속에서 유익한 효과를 내는 모든 균을 의미한다. 장뿐만 아니라 면역질환 등 다양한 신체 기능에 관여한다. 한동안 프로 바이오틱스의 먹이가 되는 식이섬유(프리 바이오틱스)를 함께 복용하면 효과가 더 좋다는 연구가 급물살을 탔는데, 최근에는 프로 바이오틱스가 내놓는 대사산물인 포스트 바이오틱스가 치료제나 질병 진단에 효율적이라는 주장이 주목받고 있다.Probiotics refer to all bacteria that have beneficial effects in the body, including lactic acid bacteria. It is involved in various bodily functions such as the intestine as well as immune diseases. For a while, research showing that taking together dietary fiber (prebiotics), which is the food of probiotics, has a better effect has been on the rise. The claim is receiving attention.
차전자피는 식이섬유가 풍부하며, 물을 만나면 40배 가량 팽창하면서 점성을 만들어 낼 수 있고, 장의 연동운동을 촉진시키고 변을 부드럽게 해주며 장내 독소들을 흡착하여 편안하게 쾌변을 도와줄 수 있다. 또한, 차전자피는 혈압을 낮출 수 있고, 콜레스테롤 수치 조절, 심장근육을 강화시켜 심장병의 위험을 낮출 수 있다.Psyllium hull is rich in dietary fiber, and when it comes into contact with water, it can expand 40 times to create viscosity, promote intestinal peristalsis, soften stool, and absorb intestinal toxins to help with comfortable bowel movements. In addition, psyllium can lower blood pressure, control cholesterol levels, and strengthen the heart muscle, thereby lowering the risk of heart disease.
본 발명은 사용하고자 하는 식재료인 차전자피를 이용한 포스트 바이오틱스 제조 방법으로서 차전자피를 사용할 수 있다. 차전자피와 프로 바이오틱스를 이용하여 발효시킬 수 있고, 대사산물인 포스트 바이오틱스를 건조시킨 분말을 제조할 수 있으며, 이러한 분말을 복용하면, 다양한 질병의 유발을 억제할 수 있다.In the present invention, psyllium hull can be used as a method for producing post-biotics using psyllium hull, which is a food material to be used. It can be fermented using psyllium hull and probiotics, and a dried powder of post-biotics, a metabolite, can be prepared.
도 1은 본 발명의 제조 방법에 관한 블럭도이다.1 is a block diagram of a manufacturing method of the present invention.
최근 장 건강에 대한 사람들의 관심이 높아지면서 건강보조식품으로 유산균 제품, 즉 프로 바이오틱스, 프리 바이오틱스, 신 바이오틱스, 포스트 바이오틱스 등을 섭취하는 사람들이 늘고 있다.Recently, as people's interest in gut health has increased, more and more people are taking lactic acid bacteria products as health supplements, such as probiotics, prebiotics, new biotics, and post-biotics.
꾸준한 유산균 섭취는 장내 유익균을 늘리고 유해균을 줄여 장 내 환경을 건강한 상태로 유지할 수 있게 되는데, 시장에 내온 유산균 제품에 사용된 유산균들이 다양한 만큼 그 종류를 알고 먹는 것이 더 효과적으로 장 건강을 관리하는 데 도움이 될 수 있다.Consistent intake of lactic acid bacteria increases the beneficial bacteria in the intestine and reduces harmful bacteria to keep the intestinal environment in a healthy state. this can be
먼저 프로 바이오틱스는 유익균이 많은 장 내 환경을 만들 수 있도록 도움을 주는 유산균으로, 장 점막에 정착해 유해균으로부터 장을 보호하는 역할을 한다.First, probiotics are lactic acid bacteria that help create an intestinal environment with many beneficial bacteria.
장 환경을 균형있게 조성해 장 건강과 면역 시스템을 강화할 수 있지만, 암 환자, 크론병 환자, 면역억제제 복용자 등은 패혈증이나 염증이 생길 수 있기 때문에 섭취에 주의가 필요하다.It can strengthen intestinal health and immune system by creating a balanced intestinal environment, but cancer patients, Crohn's disease patients, and immunosuppressive drug users may develop sepsis or inflammation.
프리 바이오틱스는 프로 바이오틱스의 먹이 역할을 하는 것으로, 최근 유익균의 먹이가 장 건강에 도움이 된다는 것이 방송 등을 통해 널리 알려지기도 했다.Prebiotics serve as food for probiotics, and it has recently been widely known through broadcasting that the food of beneficial bacteria is beneficial to gut health.
프리 바이오틱스는 프락토올리고당을 포함할 수 있다. 프리 바이오틱스는 음식으로도 섭취를 할 수 있는데, 대표적으로 바나나, 마, 콩, 양파, 사과, 옥수수, 다크 초콜릿(70% 이상) 등에 다량 함유돼 있다.Prebiotics may include fructooligosaccharides. Prebiotics can be consumed through food, and they are typically found in bananas, hemp, soybeans, onions, apples, corn, and dark chocolate (more than 70%) in large amounts.
다만, 지나치게 섭취하면 경우에 따라 가스가 차거나 구토감을 느낄 수 있으니 적정량을 섭취하는 것이 중요하다.However, if you consume too much, you may feel gas or vomiting in some cases, so it is important to consume an appropriate amount.
신 바이오틱스는 프로 바이오틱스와 프리 바이오틱스의 장점을 모두 취하기 위해서 둘을 혼합한 제품이다. Synbiotics are a blend of probiotics and prebiotics to take advantage of both.
포스트 바이오틱스는 유산균 배양 건조물을 말한다. 유산균이 만들어내는 유기산, 즉 박테리오신(Bacteriocin), 뷰틸레이트, 효소, 아미노산, 펩타이드 등 유산균 대사산물이 대표적이다.Post-biotics refer to lactic acid bacteria culture dried products. Organic acids produced by lactic acid bacteria, that is, lactic acid bacteria metabolites such as bacteriocin, butylate, enzymes, amino acids, and peptides, are representative.
포스트 바이오틱스는 유해균을 억제하고 장 점막 면역을 활성화시키는데, 프로 바이오틱스의 경우 장 점막에 부착해 유해균을 억제하는데 일정한 시간이 필요하지만, 포스트 바이오틱스는 장 점막 부착의 과정을 생략할 수 있는 것이 장점이다.Post-biotics suppress harmful bacteria and activate intestinal mucosal immunity. In the case of probiotics, it takes a certain amount of time to adhere to the intestinal mucosa to suppress harmful bacteria, but post-biotics can omit the process of intestinal mucosal adhesion. advantage.
최근 미국이나 유럽에서는 이같은 유산균 대사산물에 대한 관심도가 높아지면서 포스트 바이오틱스가 상품화돼 이를 섭취하는 사람들이 늘고 있다.Recently, as interest in lactic acid bacteria metabolites has increased in the United States and Europe, post-biotics have been commercialized and more and more people are taking them.
포스트 바이오틱스는 장 기능 개선을 통한 예방 건강과 건강 증진에 도움을 주기 때문에 세균성 장 질환자, 잦은 배탈, 비만, 당뇨, 다이어트가 필요한 사람 등에게 도움이 될 수 있다.Because post-biotics help to promote preventive health and health by improving intestinal function, it can be helpful for people with bacterial bowel disease, frequent stomach upsets, obesity, diabetes, and those who need a diet.
포스트 바이오틱스는 프로 바이오틱스가 먹이인 프리 바이오틱스를 먹고 만들어내는 대사 산물로 유기산, 박테리오신(Bacteriocin), EPS, 효소 등을 나타낸다.Postbiotics are metabolites produced by eating prebiotics, the food of which probiotics are fed, and represent organic acids, bacteriocins, EPS, and enzymes.
포스트 바이오틱스는 '프로 바이오틱스’와 그의 먹이인 ‘프리 바이오틱스’, 이 둘을 함께 지칭하는 ‘신 바이오틱스’에 이어 4세대 유산균으로 주목받고 있다.Post-biotics are attracting attention as the fourth-generation lactic acid bacteria following 'probiotics' and 'prebiotics', which are their food, and 'new biotics', which refer to both.
프로 바이오틱스는 유산균을 비롯해 몸속에서 유익한 효과를 내는 모든 균을 의미한다. 장뿐만 아니라 면역질환 등 다양한 신체 기능에 관여한다. 한동안 프로 바이오틱스의 먹이가 되는 식이섬유(프리 바이오틱스)를 함께 복용하면 효과가 더 좋다는 연구가 급물살을 탔는데, 최근에는 프로 바이오틱스가 내놓는 대사산물인 포스트 바이오틱스가 치료제나 질병 진단에 효율적이라는 주장이 주목받고 있다.Probiotics refer to all bacteria that have beneficial effects in the body, including lactic acid bacteria. It is involved in various bodily functions such as the intestine as well as immune diseases. For a while, research showing that taking together dietary fiber (prebiotics), which is the food of probiotics, has a better effect has been on the rise. The claim is receiving attention.
차전자피는 식이섬유가 풍부하며, 물을 만나면 40배 가량 팽창하면서 점성을 만들어 낼 수 있고, 장의 연동운동을 촉진시키고 변을 부드럽게 해주며 장내 독소들을 흡착하여 편안하게 쾌변을 도와줄 수 있다. 또한, 차전자피는 혈압을 낮출 수 있고, 콜레스테롤 수치 조절, 심장근육을 강화시켜 심장병의 위험을 낮출 수 있다.Psyllium hull is rich in dietary fiber, and when it comes into contact with water, it can expand 40 times to create viscosity, promote intestinal peristalsis, soften stool, and absorb intestinal toxins to help with comfortable bowel movements. In addition, psyllium can lower blood pressure, control cholesterol levels, and strengthen the heart muscle, thereby lowering the risk of heart disease.
본 발명은 차전자피를 이용한 포스트 바이오틱스 제조 방법으로서, 차전자피와 프로 바이오틱스를 이용하여 발효시킬 수 있고, 대사산물인 포스트 바이오틱스를 건조시킨 분말을 제조할 수 있으며, 이러한 분말을 복용하면, 다양한 질병의 유발을 억제할 수 있다.The present invention is a method for producing post-biotics using psyllium hull, which can be fermented using psyllium husk and probiotics, and a powder obtained by drying post-biotics, a metabolite, can be prepared. can suppress the induction of
프로 바이오틱스는 당류를 발효하여 에너지를 획득하고 다량의 유산을 생성하는 세균으로서 단백질을 분해하지만 부패시키는 능력이 없으며 인체에 해로운 물질을 생성하지 않고 유익한 작용을 하는 세균들 중 유산을 생산하는 균주를 의미한다. 유산균은 건강한 사람들의 장내 미생물 생태계의 이부로 존재하여 면역기작의 증강에 의해 사람의 면역력을 강화하는 역할을 한다.Probiotics are bacteria that ferment sugars to obtain energy and produce a large amount of lactic acid. Probiotics are bacteria that break down proteins but do not have the ability to decay, do not produce harmful substances to the human body, and have beneficial effects among bacteria that produce lactic acid. it means. Lactobacillus exists as a part of the intestinal microbial ecosystem of healthy people and plays a role in strengthening human immunity by enhancing immune mechanisms.
이러한 유산균에 차전자피를 적정비율로 혼합하여 유산균 발효액을 만들 수 있다. 또한 유산균 발효액은 장기 보관에는 적합하지 않기 때문에 본 발명의 제조 방법은 이를 보완하여 장기 보관할 수 있는 포스트 바이오틱스 분말을 제조하는 방법을 제시하고자 한다.The lactic acid bacteria fermentation broth can be made by mixing psyllium husks with these lactic acid bacteria in an appropriate ratio. In addition, since the fermentation broth of lactic acid bacteria is not suitable for long-term storage, the manufacturing method of the present invention is to supplement this and propose a method for manufacturing a post-biotic powder that can be stored for a long time.
차전자피를 발효하여 포스트 바이오틱스를 제조할 수 있다. 차전자피는 질경이 씨앗(차전자)의 껍질(피)로 대변의 양을 늘려 변비를 치료하는 약물이다. 변비 치료제로 쓰이는 약물을 하제라고 하는데, 차전자피는 팽창성 하제로 분류된다. 차전자피는 80% 이상이 식이섬유로 이루어져 있으며 물에 녹는 수용성과 물에 녹지 않는 불용성 식이섬유가 골고루 함유되어 있다. Post-biotics can be produced by fermenting psyllium hulls. Psyllium hull is a drug that treats constipation by increasing the amount of feces with the bark (blood) of plantain seeds (psyllium). A drug used to treat constipation is called a laxative, and psyllium is classified as an expansive laxative. More than 80% of psyllium hull consists of dietary fiber, and contains both water-soluble and water-insoluble dietary fiber.
수용성 식이섬유는 장내 찌꺼기를 흡착하고 대변의 점도를 높여 대변의 이동성을 증가시키는 작용을 하며, 불용성 식이섬유는 수분을 흡수하여 대변의 크기를 늘려주고 대장 통과시간을 단축시켜 변비를 개선한다. Soluble fiber absorbs intestinal waste and increases stool viscosity to increase stool mobility, while insoluble fiber absorbs water to increase stool size and shorten colon transit time to improve constipation.
차전자피는 충분한 양의 물을 섭취해야 효과가 나타나며, 몸에 흡수되지 않고 생리현상과 가장 비슷하게 배변을 촉진하여 변비 환자들에게 추천된다. Psyllium hull is effective only when a sufficient amount of water is consumed, and it is not absorbed by the body and promotes bowel movements most similar to menstruation, so it is recommended for constipation patients.
또한 차전자피는 콜레스테롤 개선 및 배변활동을 원활하게 하는 건강기능식품의 기능성 원료로도 사용되며, 기능성 원료로 사용될 때에는 차전자피식이섬유라고 불린다.Psyllium hull is also used as a functional raw material for health functional food that improves cholesterol and facilitates bowel movements.
[제1 실시 예][First embodiment]
1. 제1 액상물을 배양하는 단계로서, 김치로부터 추출하여 배양한 유산균 Weissella paramesenteroides, Lactobacillus paraplantarum, Lactobacillus sakei subsp. Sakei, Leuconostoc mesenteroides, Leuconostoc gasicomitatum, Lactobacillus brevis, Lactobacillus sakei, Enterococcus faecium, Leuconosotoc kimchii, Leuconostoc citreum, Lactobacillus plantarum 중 적어도 어느 하나를 식품용 유산균 액상배지에 접종하여 제1 액상물을 대량 배양할 수 있다.1. In the step of culturing the first liquid, lactic acid bacteria Weissella paramesenteroides, Lactobacillus paraplantarum, Lactobacillus sakei subsp. At least one of Sakei, Leuconostoc mesenteroides, Leuconostoc gasicomitatum, Lactobacillus brevis, Lactobacillus sakei, Enterococcus faecium, Leuconosotoc kimchii, Leuconostoc citreum, and Lactobacillus plantarum can be inoculated in a large amount of food-grade lactic acid bacteria liquid medium 1
그중에서도 특히 Lactobacillus plantarum의 변종인 Lactobacillus plantarum MB01, Leuconostoc kimchii의 변종인 Leuconostoc kimchii MB1220,Leuconostoc citreum의 변종인 Leuconostoc citreum MB1221을 사용하여 배양 했을 때의 결과는 다른 균들을 배양을 했을 때 보다 뛰어날 수 있다.Among them, the results of culturing using Lactobacillus plantarum MB01, a mutant of Lactobacillus plantarum, Leuconostoc kimchii MB1220, a mutant of Leuconostoc kimchii, and Leuconostoc citreum MB1221, a mutant of Leuconostoc citreum can be superior to those of other bacteria.
Lactobacillus plantarum의 변종인 Lactobacillus plantarum MB01의 경우 비만 예방, 면역력 강화, 숙취 해소 등의 기능성이 확인되었다.In the case of Lactobacillus plantarum MB01, a mutant of Lactobacillus plantarum, functions such as preventing obesity, strengthening immunity, and relieving hangover were confirmed.
Leuconostoc kimchii의 변종인 Leuconostoc kimchii MB1220의 경우 숙취해소, 소화촉진, 면역강화, 항균, 항암, 혈중 콜레스트롤 저하, 노화 억제 등의 기능이 관련되어진 유산균으로 알려져 있다.In the case of Leuconostoc kimchii MB1220, a variant of Leuconostoc kimchii, it is known as a lactic acid bacterium with functions such as hangover relief, digestion promotion, immune enhancement, antibacterial, anticancer, blood cholesterol lowering, and aging inhibition.
Leuconostoc citreum의 변종인 Leuconostoc citreum MB1221의 경우 장출혈성 대장균을 사멸시킨다는것으로 알려져 있다.Leuconostoc citreum MB1221, a variant of Leuconostoc citreum, is known to kill enterohaemorrhagic Escherichia coli.
배양된 상기 제1 액상물에는 유산균의 발효과정 중 생성된 대사산물로서 아미노산, 펩타이드, 비타민 및 유기산 등이 포함될 수 있다.The cultured first liquid may include amino acids, peptides, vitamins and organic acids as metabolites generated during the fermentation process of lactic acid bacteria.
김치 유산균을 이용하면 한국인의 체질에 맞는 발효 결과를 달성할 수 있고, 유산균의 발효 상태도 인체에 무해할 수 있으며, 한국인의 소화 기작에 잘 어울릴 확률을 높일 수 있다.By using kimchi lactic acid bacteria, fermentation results suitable for the constitution of Koreans can be achieved, the fermentation state of lactic acid bacteria can also be harmless to the human body, and the probability that it will suit the digestive mechanism of Koreans can be increased.
배양 조건은 배양 온도 20~40℃에서 12~48시간 동안 유산균 수가 1x108 cfu/ml 이상, pH 5.0 이하가 될 때까지 배양하는 것이 바람직할 수 있다. 실험 결과, 유산균의 수가 너무 많으면 배양 상태가 급속 진전되어 변질될 수 있고, 유산균의 수가 너무 작으면 배양 시간이 너무 지연될 수 있다.In culture conditions, it may be preferable to incubate at a culture temperature of 20 to 40° C. for 12 to 48 hours until the number of lactic acid bacteria becomes 1x10 8 cfu/ml or more and pH 5.0 or less. As a result of the experiment, if the number of lactic acid bacteria is too large, the culture state may be rapidly developed and deteriorated, and if the number of lactic acid bacteria is too small, the culture time may be too delayed.
2. 다음의 표 1을 참조하면, 액상배지 조성물은 포도당 2.0 ~ 4.0%, 소이펩톤 0.5 ~ 2.0%, 효모추출물 0.2 ~ 1.0%, 기타 2~4%의 조성으로 마련될 수 있다.2. Referring to Table 1 below, the liquid medium composition may be prepared in a composition of 2.0 to 4.0% glucose, 0.5 to 2.0% soipeptone, 0.2 to 1.0% yeast extract, and 2 to 4% other.
3. 제2 액상물을 제조하는 단계로서, 배양된 상기 제1 액상물과, 첨가물을 넣지 않은 원물을 착즙기에 넣어 만든 착즙액과, 차전자피는 차전자의 껍질을 분쇄하여 식용에 적합하도록 만든 것으로서, 첨가물을 넣지 않은 차전자피를 분쇄기에 넣어 만든 분말을 혼합하여 제2 액상물을 마련할 수 있다.3. A step of preparing the second liquid, wherein the cultured first liquid, the juice prepared by putting the raw material without additives into a juicer, and the psyllium hull are made suitable for edible use by crushing the skin of psyllium. , it is possible to prepare a second liquid product by mixing the powder made by putting psyllium hulls without additives into a grinder.
4. 상기 제2 액상물을 배양하여 발효액을 제조하는 단계로서, 상기 제2 액상물을 배양실에 넣고 20~40℃에서 24~48시간 정도 발효시켜 발효액을 마련할 수 있다. 사용하는 원물에 따라 조성물의 비율은 변경될 수 있고 또한 발효과정이 늘어날 수도 있다.4. In the step of preparing a fermentation broth by culturing the second liquid, the second liquid may be placed in a culture chamber and fermented at 20 to 40° C. for 24 to 48 hours to prepare a fermentation broth. Depending on the raw material used, the proportion of the composition may be changed and the fermentation process may be increased.
사용하는 각각의 원물에 따라, 조성물의 비율이 최적 설계되어야 한다. 예를 들어, A라는 원물은 많은 양을 넣어야 제조시 원하는 농도와 색 등이 나올 수 있고, B 라는 원물은 조금만 사용하여도 제조시 원하는 농도와 색 등이 나올 수 있다. 이와 같이 사용하고자 하는 원물의 특징에 따라 유산균의 발효 결과가 달라질 수 있기 때문에 상기와 같은 최적 혼합비를 결정하였다.According to each raw material used, the proportion of the composition should be optimally designed. For example, the desired concentration and color can be obtained when the raw material A is put in a large amount, and the desired concentration and color can be obtained when the raw material B is used even with a small amount. As such, the optimum mixing ratio as described above was determined because the fermentation results of lactic acid bacteria may vary depending on the characteristics of the raw material to be used.
예를 들면, 김치에서 유래한 Weissella paramesenteroides, Lactobacillus paraplantarum, Lactobacillus sakei subsp. Sakei, Leuconostoc mesenteroides, Leuconostoc gasicomitatum, Lactobacillus brevis, Lactobacillus sakei, Enterococcus faecium, Leuconosotoc kimchii, Leuconostoc citreum, Lactobacillus plantarum 유산균에 정제수와 유산균의 먹이를 넣고 배지를 형성 후 24시간~72시간 배양할 수 있다. 배양 진행시 원하는 발효물질인, 아보카도, 강황, 레몬, 라임, 새싹보리, 브로콜리, 샐러리, 파슬리, 케일, 석류, 아로니아, 노니, 바나나, 풋사과, 파인애플, 레드자몽, 오렌지, 깔라만시, 체리, 블루베리, 당근, 꽃송이버섯, 침향, 차가버섯, 타트체리, 엘더베리 등 어느 과채류를 사용해서 배양하여도 무방하다.For example, Weissella paramesenteroides, Lactobacillus paraplantarum, and Lactobacillus sakei subsp. Sakei, Leuconostoc mesenteroides, Leuconostoc gasicomitatum, Lactobacillus brevis, Lactobacillus sakei, Enterococcus faecium, Leuconosotoc kimchii, Leuconostoc citreum, Lactobacillus plantarum Lactobacillus can be cultured for 24 hours to 72 hours after adding purified water and lactobacillus feed. Avocado, turmeric, lemon, lime, sprouted barley, broccoli, celery, parsley, kale, pomegranate, aronia, noni, banana, green apple, pineapple, red grapefruit, orange, calamansi, cherry , blueberries, carrots, chrysanthemum mushrooms, chives, chaga mushrooms, tart cherries, elderberries, etc. may be used for culturing.
5. 상기 발효액을 건조하여 제3 액상물을 제조하는 단계로서, 상기 제2 액상물이 발효과정이 끝난 후, 건조기를 사용하여 제3 액상물을 제조할 수 있다.5. As a step of manufacturing a third liquid by drying the fermentation broth, after the second liquid is fermented, a third liquid may be prepared using a dryer.
6. 건조된 상기 제3 액상물을 열처리하고 분쇄하여 분말형태의 차전자피 포스트바이오틱스를 제조하는 단계로서, 상기 제3 액상물을 70℃ 이상 온도에서 30분 이상 열처리시킬 수 있다.6. A step of heat-treating and pulverizing the dried third liquid material to produce powdered psyllium hull postbiotics, wherein the third liquid material may be heat-treated at a temperature of 70° C. or higher for 30 minutes or longer.
이는 살아있는 유산균이 우리 몸에 들어갔을 때 부작용을 유발할 수 있는 위험을 예방하고자 함이다. 본 발명에서 상기 열처리 온도 및 시간은 최적화되었다. 너무 높은 온도에서 하게 될 경우 제품이 변색이 될 수 있고 70℃ 이상 에서 했을 경우 변색의 위험이 가장 적었기 때문에 상기 온도에서 진행할 수 있다. 너무 오랜 시간을 할 경우 역시 변색의 위험이 나타날 수 있다This is to prevent the risk that live lactic acid bacteria can cause side effects when they enter our body. In the present invention, the heat treatment temperature and time were optimized. If it is done at too high a temperature, the product may be discolored, and if it is done at 70° C. or higher, the risk of discoloration is the least, so it can proceed at the above temperature. If you do it for too long, there is also a risk of discoloration.
열처리가 끝난 상기 제3 액상물을 분쇄하여 분말 형태의 차전자피 포스트 바이오틱스를 만들 수 있다. By pulverizing the third liquid material after the heat treatment has been completed, it is possible to produce psyllium hull post-biotics in a powder form.
Claims (5)
원물을 착즙기에 넣어 원물 착즙액을 만드는 단계;
차전자피를 분쇄기에 넣어 차전자피 분말을 준비하는 단계;
상기 제1 액상물, 상기 원물 착즙액 및 상기 차전자피 분말을 혼합하여 제2 액상물로 만드는 단계;
상기 제2 액상물을 배양하여 발효액을 만드는 단계;
상기 발효액을 건조하여 제3 액상물을 만드는 단계;
상기 제3 액상물을 열처리하고 분쇄하여 분말 형태의 차전자피 포스트 바이오틱스를 만드는 단계;
를 포함하는 차전자피 포스트 바이오틱스 제조방법.
Inoculating the lactic acid bacteria into the liquid medium and culturing the first liquid containing the lactic acid bacteria liquid;
Putting the raw material into a juicer to make a raw material juice;
preparing psyllium hull powder by putting the psyllium hull into a grinder;
mixing the first liquid, the raw juice, and the psyllium hull powder to make a second liquid;
culturing the second liquid to make a fermentation broth;
drying the fermentation broth to make a third liquid;
heat-treating and pulverizing the third liquid to produce psyllium hull post-biotics in powder form;
A method for producing psyllium hull post-biotics comprising a.
상기 유산균은 김치로부터 추출된 유산균인 Weissella paramesenteroides, Lactobacillus paraplantarum, Lactobacillus sakei subsp. Sakei, Leuconostoc mesenteroides, Leuconostoc gasicomitatum, Lactobacillus brevis, Lactobacillus sakei, Enterococcus faecium, Leuconosotoc kimchii, Leuconostoc citreum, Lactobacillus plantarum 중 적어도 어느 하나를 포함하고,
상기 액상배지는 포도당 2.0 ~ 4.0%, 소이펩톤 0.5 ~ 2.0%, 효모추출물 0.2 ~ 1.0%를 포함하며,
상기 액상배지에서 상기 유산균을 배양시키는 차전자피 포스트 바이오틱스 제조 방법.
According to claim 1,
The lactic acid bacteria were extracted from kimchi, Weissella paramesenteroides, Lactobacillus paraplantarum, Lactobacillus sakei subsp. Contains at least one of Sakei, Leuconostoc mesenteroides, Leuconostoc gasicomitatum, Lactobacillus brevis, Lactobacillus sakei, Enterococcus faecium, Leuconosotoc kimchii, Leuconostoc citreum, and Lactobacillus plantarum;
The liquid medium contains glucose 2.0 ~ 4.0%, soy peptone 0.5 ~ 2.0%, yeast extract 0.2 ~ 1.0%,
A method for producing psyllium hull post biotics for culturing the lactic acid bacteria in the liquid medium.
상기 유산균은 상기 김치로부터 추출된 유산균인 Lactobacillus plantarum의 변종인 Lactobacillus plantarum MB01, Leuconostoc kimchii의 변종인 Leuconostoc kimchii MB1220,Leuconostoc citreum의 변종인 Leuconostoc citreum MB1221중 적어도 어느 하나를 포함하는 차전자피 포스트 바이오틱스 제조 방법.
According to claim 1,
The method of claim 1, wherein the lactic acid bacteria comprises at least one of Lactobacillus plantarum MB01, a strain of Lactobacillus plantarum, which is a lactic acid bacterium extracted from the kimchi, Leuconostoc kimchii MB1220, a strain of Leuconostoc kimchii, and Leuconostoc citreum MB1221, a strain of Leuconostoc citreum.
상기 발효액을 만드는 단계는 상기 제2 액상물을 배양실에 넣고 20~40℃에서 24~48시간 정도 발효시키는 차전자피 포스트 바이오틱스 제조 방법.
According to claim 1,
In the step of making the fermentation broth, the second liquid material is placed in a culture chamber and fermented at 20-40° C. for 24-48 hours.
상기 분말 형태의 차전자피 포스트바이오틱스를 만드는 단계는,
상기 제3 액상물을 70℃ 이상 온도에서 30분 이상 열처리하고, 열처리가 끝난 상기 제3 액상물을 분쇄하여 분말 형태의 차전자피 포스트 바이오틱스를 만드는 차전자피 포스트 바이오틱스 제조 방법.According to claim 1,
The step of making the psyllium hull postbiotics in powder form,
A method of manufacturing psyllium hull post-biotics in which the third liquid material is heat-treated at a temperature of 70° C. or higher for 30 minutes or more, and the third liquid material after heat treatment is pulverized to produce psyllium hull post-biotics in powder form.
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