CN114468280A - Enzyme for preventing obesity and regulating intestinal flora and preparation method and application thereof - Google Patents

Abstract

A ferment for preventing obesity and regulating intestinal flora and a preparation method and application thereof are disclosed, wherein the ferment is prepared by fermenting the following raw materials: 20-40 parts of aloe fermentation stock solution, 40-60 parts of comprehensive fruit and vegetable fermentation stock solution, 1-15 parts of fructo-oligosaccharide, 1-15 parts of galacto-oligosaccharide and 1-10 parts of trehalose. The ferment can remarkably reduce the weight growth rate, the weight of epididymal fat and perirenal fat, can increase the abundance of intestinal flora, and is beneficial to the proliferation of beneficial bacteria and the inhibition of harmful bacteria.

Description

Enzyme for preventing obesity and regulating intestinal flora and preparation method and application thereof

Technical Field

The invention belongs to the field of health care products. In particular to a ferment for preventing obesity and regulating intestinal flora and a preparation method and application thereof.

Background

The definition of the edible plant enzyme in QB/T5323-2018 plant enzyme, applied by West 1/7 of the Ministry of industry and communications in 2019, is as follows: the plant which can be used for food processing is taken as a main raw material, and an auxiliary material is added or not added, and the product which contains a specific bioactive component and can be eaten by human beings is prepared by microbial fermentation. Almost all cellular processes require enzymes, which are essential for maintaining the normal functions of the body, digesting food, repairing tissues, etc. The main functions of the ferment are: regulating internal environment, and purifying toxin in vivo; the intestinal flora is kept balanced; improving the intestinal peristalsis; strengthening cell function, promoting metabolism, and enhancing immunity.

The human body mainly synthesizes enzymes through food intake and self-synthesis. With the increasing age, the secretion of enzymes in the body is less and less, and meanwhile, the modern people are overnourished and residual toxins in the body cause endocrine dyscrasia and metabolic disorders, so that more enzymes need to be taken from food to maintain the metabolism of the body. However, as food materials are increasingly contaminated, and more elaborate processing methods are used, enzymes in the food are destroyed. Therefore, additional supplementation with ferments is essential for maintaining physical health.

In view of this, the invention is particularly proposed.

Disclosure of Invention

The first object of the present invention is to provide a ferment for preventing obesity and regulating intestinal flora, which can significantly reduce the weight gain rate, the weight of epididymal fat and perirenal fat, increase the abundance of intestinal flora, and contribute to the proliferation of beneficial bacteria and the inhibition of harmful bacteria.

The second objective of the present invention is to provide a method for preparing the above enzyme for preventing obesity and regulating intestinal flora.

The third objective of the present invention is to provide an application of the above enzyme for preventing obesity and regulating intestinal flora in the preparation of a medicament or food for preventing obesity and regulating intestinal flora.

In order to achieve the purpose, the invention adopts the following technical scheme:

a ferment for preventing obesity and regulating intestinal flora is prepared by fermenting the following raw materials: 20-40 parts of aloe fermentation stock solution, 40-60 parts of comprehensive fruit and vegetable fermentation stock solution, 1-15 parts of fructo-oligosaccharide, 1-15 parts of galacto-oligosaccharide and 1-10 parts of trehalose.

The ferment for preventing obesity and regulating intestinal flora as described above is preferably prepared by fermenting the following raw materials: 15-25 parts of aloe fermentation stock solution, 45-55 parts of comprehensive fruit and vegetable fermentation stock solution, 3-12 parts of fructo-oligosaccharide, 3-12 parts of galacto-oligosaccharide and 2-8 parts of trehalose.

The ferment for preventing obesity and regulating intestinal flora as described above, preferably, the aloe is at least one of aloe vera, aloe arborescens and aloe vera.

The ferment for preventing obesity and regulating intestinal flora as described above is preferably prepared from aloe vera gel which is prepared from the leaves of aloe vera by leaching off quinone, cleaning, peeling, blanching and sterilizing.

The ferment for preventing obesity and regulating intestinal flora as described above preferably comprises the following raw materials: lemon, green plum, papaya, kiwi fruit, mulberry, medlar, red date and/or rose.

The ferment for preventing obesity and regulating intestinal flora as described above, preferably, the fruit and vegetable raw materials of the integrated fruit and vegetable fermentation stock solution further include: pineapple, plum, grape, dragon fruit, apple, hawthorn, green tea, okra, corn, mushroom, prune, noni, dark plum, citrus, grapefruit, and/or blueberry.

The preparation method of the ferment for preventing obesity and regulating intestinal flora as described above preferably comprises the following steps:

fermentation in the first stage: fermenting the aloe and the fruit and vegetable raw materials at normal temperature for 3 months to 2 years respectively;

and (3) second-stage fermentation: mixing the aloe and fruit and vegetable fermentation stock solution obtained in the first stage fermentation with the rare sugar according to the weight ratio, and fermenting by adopting lactobacillus plantarum and/or saccharomycetes at the fermentation temperature of 25-36 ℃ for 7-30 days.

The use of the ferment for preventing obesity and regulating intestinal flora as described above in the preparation of a medicament or food for preventing obesity and regulating intestinal flora function.

The weight parts of the aloe fermentation raw liquid, typically but not limited to, for example, may be 20 parts, 21 parts, 22 parts, 23 parts, 24 parts, 25 parts, 26 parts, 27 parts, 28 parts, 29 parts, 30 parts, 31 parts, 32 parts, 33 parts, 34 parts, 35 parts, 36 parts, 37 parts, 38 parts, 39 parts or 40 parts, and the like.

The total weight parts of the fruit and vegetable fermentation stock solution can be, for example, 40 parts, 41 parts, 42 parts, 43 parts, 44 parts, 45 parts, 46 parts, 47 parts, 48 parts, 49 parts, 50 parts, 51 parts, 52 parts, 53 parts, 54 parts, 55 parts, 56 parts, 57 parts, 58 parts, 59 parts or 60 parts, and the like.

The weight fraction of fructooligosaccharide, which is typical but not limiting, may be, for example, 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, or 15 parts, and the like.

The weight fraction of galactooligosaccharide, which is typical but not limiting, may be, for example, 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, or 15 parts, and the like.

The trehalose may be, for example, typically but not limited to, 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, or 15 parts by weight or the like.

The invention provides a ferment for preventing obesity and adjusting intestinal flora, which takes aloe fermentation stock solution and comprehensive fruit and vegetable fermentation stock solution as main raw materials, adds three kinds of rare sugars of fructo-oligosaccharide, galacto-oligosaccharide and trehalose, organically combines various raw materials, synergizes, jointly adjusts intestinal microecology and corrects sub-health state.

Wherein, the aloe fermentation stock solution is obtained by taking edible parts of edible aloe as raw materials and performing natural fermentation and (or) single (multiple) strain multi-stage fermentation. The Aloe is leaf or juice or concentrated dried product of Aloe Barbadensis Miller, Aloe ferox Miller or other related plants of the same genus of Liliaceae. Aloe vera is considered as one of the best quality aloe, and Aloe vera gel is prepared from leaves of Aloe vera as raw material by leaching off quinone, cleaning, peeling, blanching, sterilizing, etc., and approved as a new resource food by No. 12 bulletin of Ministry of health 2008. According to the record of Chinese pharmacopoeia, aloe gel enters liver, stomach and large intestine channels, is bitter in taste and cold in nature, has the functions of clearing liver heat and relaxing the bowels, and is mainly used for treating constipation and the like.

Although the chemical components of aloe are complex, they are mainly classified into two categories in terms of specificity and efficacy: one is anthraquinone (aloin for short) such as aloin. It is the first recognized and utilized component in aloe, and is one of the main organic active components of aloe gel, and can decompose and activate intestinal bacteria, increase lipase activity, promote intestinal muscle contraction, promote intestinal peristalsis, relax bowels and promote urination, and has obvious effect of treating constipation. The other is polysaccharide, and researches show that the polysaccharide can promote the biosynthesis of protein and nucleic acid, regulate the growth of cells and improve the immunity of a human body.

The comprehensive fruit and vegetable fermentation stock solution is mainly prepared by two or more fermentation processes, is rich in various bioactive substances, and comprises the following components: vitamins, organic acids, polyphenol, polysaccharide, dietary fiber and other small molecular active ingredients and SOD enzyme, and has the functions of activating enzyme and enzyme activity and promoting metabolism.

In the application, lemon, green plum, pawpaw, kiwi fruit, mulberry, medlar, red date and/or double-petal red rose are preferably selected as raw materials, and are fermented into the comprehensive fruit and vegetable fermentation stock solution, wherein,

the lemon contains rich citric acid, is known as a citric acid warehouse, and can promote the production of body fluid, relieve summer heat, stimulate the appetite and activate the spleen; it is also rich in vitamin C, and can maintain the formation of various tissues and intercellular substance of human body and maintain their normal physiological functions. The matrix, the adhesive and the colloid in the human body need vitamin C for protection.

The green plum not only contains rich organic acid, but also can promote the discharge of toxin in vivo, and has the effects of expelling toxin and relaxing bowels.

The pawpaw contains papain, can compensate secretion of pancreas and intestinal tract, supplement insufficiency of gastric juice, and is helpful for decomposing protein and starch.

The kiwi fruit contains abundant vitamin C and a large amount of minerals, and is helpful for maintaining the electrolyte balance of the body.

Pharmacological research shows that: the mulberry entering the stomach can supplement the lack of gastric juice, promote the digestion of gastric juice, enter the intestine and promote the secretion of intestinal juice and the gastrointestinal peristalsis, thereby having the effects of tonifying and strengthening.

The fructus Lycii not only contains fructus Lycii polysaccharide, but also contains betaine, and has effects of supplying methyl in vivo, accelerating lipid metabolism, and resisting fatty liver.

The red date has very high vitamin content, has the reputation of 'natural vitamin pills', and has the effects of nourishing yin and tonifying yang.

The rose with double petals contains 18 amino acids and microelements required by human body, and has the effects of coordinating intestines and stomach and promoting metabolism.

After the 8 raw materials are fermented, macromolecular nutrient components are further decomposed into micromolecular nutrient substances which are easier to absorb by a human body, and the intestinal tract safety is not burdened.

The rare sugar refers to monosaccharide and derivatives with low content in nature, and is very rare compared with glucose and fructose existing in nature in a large amount, and about 50 types exist. The rare sugar can reduce the formation of toxic substances (such as endotoxin, ammonia and the like), has a protective effect on intestinal mucosa cells and liver, can regulate intestinal flora, allows certain microorganisms to be planted in the intestinal tract, provides a proper inhabitation environment for the microorganisms, promotes the improvement and influence of the intestinal mucosa immune function, and participates in various metabolic functions of a human body. According to the specificity of various rare sugars on the action of intestinal flora, the invention screens three rare sugars capable of promoting the proliferation of one or more specific beneficial bacteria for compounding (the action condition of the main beneficial bacteria on the intestinal tract is shown in table 1), and the three rare sugars have complementary advantages and are strong in connection, so that the aims of regulating the intestinal flora, increasing the abundance and diversity of the beneficial bacteria, improving the intestinal motility, promoting defecation, reducing the abundance of harmful bacteria, reducing the generation of endotoxin and metabolic waste and caring the health of the intestinal tract are fulfilled.

TABLE 1 Effect of three rare sugars on major beneficial bacteria in the gut

Note: "+" indicates promotion of proliferation; "-" indicates inhibition of proliferation.

The beneficial effects of the invention are as follows:

(1) the aloe fermentation stock solution and the comprehensive fruit and vegetable fermentation stock solution are prepared by fermenting natural fruits and vegetables by a two-stage process, and the aloe fermentation stock solution contains anthraquinone substances such as aloin and polysaccharide, can decompose and activate intestinal bacteria, increase the activity of lipase, promote intestinal muscle contraction and accelerate intestinal peristalsis; the comprehensive fruit and vegetable fermentation liquor contains various micromolecular active ingredients such as vitamins, organic acids, polyphenol, crude polysaccharide, dietary fiber and the like and SOD enzyme, and has the functions of exciting the enzyme and enzyme activity, promoting metabolism and improving human immunity.

(2) The addition of the selected three rare sugars can regulate intestinal flora, increase the abundance and diversity of beneficial bacteria, improve intestinal motility, promote defecation, reduce the abundance of harmful bacteria and reduce the generation of endotoxin and metabolic waste.

(3) The combination of the fermentation stock solution and the rare sugar achieves the synergistic effect, regulates the intestinal microecology and promotes the metabolism of the Newcastle disease together, so that the ferment can control the weight increase, reduce the inflammatory reaction and obviously reduce the perirenal fat and the content of serum cholesterol. Animal experiments prove that the composition has the obvious effects of reducing the weight growth rate, the weight of epididymal fat and perirenal fat, reducing the inflammation level and regulating intestinal flora.

(4) The invention adopts a two-stage fermentation process, the aloe ferment stock solution and the comprehensive fruit and vegetable fermentation stock solution are taken as base materials in the second stage of fermentation, and three kinds of rare sugar complex sugar are particularly added, so that the problems of high sugar degree and high sugar raising index of the final product caused by adding brown granulated sugar in the prior art are solved, and meanwhile, the secondary fermentation is also significant for promoting the ripening of flavor and improving the taste.

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to the following detailed description, but those skilled in the art will understand that the following described examples are some, not all, of the examples of the present invention, and are only used for illustrating the present invention, and should not be construed as limiting the scope of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.

Example 1

Aloe fermentation stock solution: processing Aloe barbadensis Miller by: high-efficiency cleaning, slicing, adding brown granulated sugar until the sugar degree is 60, fermenting at normal temperature for 6 months, and filtering to obtain the product.

Synthesizing fruit and vegetable fermentation stock solution: respectively processing lemon, green plum, pawpaw, kiwi fruit, mulberry, red date, double red rose and medlar by: efficient cleaning-slicing-adding brown granulated sugar until the sugar degree is 60, fermenting at normal temperature for 6 months until the sugar degree is below 5, filtering to obtain 8 kinds of fruit and vegetable fermentation stock solutions, and compounding according to the same parts to obtain the fruit and vegetable fermentation stock solutions.

And (3) secondary fermentation: uniformly mixing the aloe fermentation stock solution and (or) the comprehensive fruit and vegetable fermentation stock solution and (or) fructo-oligosaccharide and (or) galacto-oligosaccharide and (or) trehalose according to the proportion listed in the table 2, then implanting lactobacillus plantarum, and fermenting at 29 +/-2 ℃ for 15 days until the pH is less than or equal to 3.6 to obtain a ferment sample 1-10.

TABLE 2 weight parts of each sample raw material

Sample (I)	1	2	3	4	5	6	7	8	9	10
											Aloe fermentation stock solution	20	-	20	40	20	-	70	20	20	20
Comprehensive fruit and vegetable fermentation stock solution	50	-	40	60	50	70	-	50	50	50
											Fructo-oligosaccharide	-	8	5	15	8	8	8	-	13.33	10
Galacto-oligosaccharides	-	8	7	15	8	8	8	13.33	-	10
											Trehalose	-	4	4	10	4	4	4	6.67	6.67	-

Note: -means not added.

Experimental example experiment of effects of enzymes for preventing obesity and regulating intestinal flora on obesity-suppressing and intestinal flora-improving effects in mice

1. Experimental methods

1.1 Purchase and Adaptation of mice

175C 57BL/6J SPF male mice were purchased from Beijing Wintolite laboratory animal technology Limited at 5 weeks of age and were acclimatized in the central barrier environment of laboratory animals at the university of south Jiangnan for 1 week using maintenance feed. One week later, the mice were numbered and randomly divided into 2 groups according to body weight, 10 mice were given maintenance diet as a blank control group (ND group), and the remaining 165 mice were given high fat diet as a model group.

1.2 Molding screening

High-fat diet was given to the mice of the model group, the food intake, food scatter, food remaining amount, water intake were recorded weekly, the body weight was weighed 1 time, and the food intake, total calorie intake and food utilization rate were calculated therefrom. After 2 weeks of feeding, the mice in the model group were ranked according to weight gain, and mice resistant to obesity were eliminated.

1.3 administration of test samples

And randomly dividing the screened 110 obesity sensitive mice into 11 groups according to the body weight, wherein the 11 groups are respectively a model control group and a sample 1-sample 10 group.

Mice were raised in a single cage on day 0, and their feces were collected and placed in sterilized EP tubes, rapidly frozen in liquid nitrogen, and stored in a-80 deg.C freezer for further use. The model control group, the positive control group and 10 sample groups prepared in example 1 were fed with high-fat diet and the empty control group with maintenance diet, respectively. Mice in groups S1, S2, S3, S4, S5, S6, S7, S8, S9 and S10 were gavaged with 10 samples of the complex prepared in the examples (7.8. mu.L/g), and the model control group and the blank control group were gavaged with the same amount of physiological saline. At the same time, the mice were subjected to gastric lavage for 6 weeks continuously, the food intake, food scattering, food remaining, and water intake were recorded every week, and the body weight was weighed 1 time, from which the food intake, total calorie intake, and food utilization rate were calculated. After week 6, mice in the blank control group, model control group, and sample 1 to sample 10 groups were subjected to CO₂Euthanasia treatment and dissection were performed, perirenal fat and epididymal adipose tissue were weighed, and colon contents were taken and placed in a sterilized EP tube, rapidly frozen in liquid nitrogen, and stored in a-80 flash freezer for further use.

1.416S rRNA sequencing

16S rRNA sequencing is carried out on the obtained fecal sample, after a sequencing result is received, effective sequences are screened through QIIME, FLASH and USEARCH software, OTU division is carried out through a UCLUST comparison tool by taking 97% similarity as a threshold value, rare OTU is removed, and the OTU used for analysis is obtained after the sequence is subjected to level extraction treatment. And the representative sequence with the highest richness is used for classification, identification and analysis, an OUT abundance matrix is generated at the same time, a Green genes database is used for comparison during the taxonomic identification of the flora, and the common OUT quantity among different treatment groups is calculated through R software.

Drawing an abundance grade curve according to the OTU abundance distribution of each sample, and calculating an alpha diversity index: simpson, Shannon, Chao 1 and ACE index.

2. Results and analysis of the experiments

2.1 mouse weight Condition

The weight change of the mice is shown in table 3, after the mice are fed with the high-fat feed and the experimental samples for 6 weeks, the weight of the mice in each experimental group is obviously different from that in the model group, the average weight of the mice in the normal group is 24.391g, and the weight of the obese mice reaches 35.936g, which indicates that the molding is successful. The body weight of the sample 2 group was significantly reduced compared to the model group, and the body weight of each of the remaining groups was extremely significantly reduced. The raw materials of sample 2 group did not contain two fermentation stocks, indicating that the two fermentation stocks play a major role in reducing the body weight of obese mice. Meanwhile, compared with the model group, the body weight reduction degree of the sample 1 group containing only two fermentation stock solutions in the raw materials is lower than that of the sample 3-10 groups, which shows that the compounding of the fermentation stock solutions and the rare sugar has the synergistic effect. Compared with the model group, the body weight average of the mice of the sample 3-5 groups is remarkably reduced (p is less than 0.0001), and the reduction degree is better than that of the mice of the sample 6-10 groups. The raw materials of the samples 3-5 consist of two fermentation stock solutions and three kinds of rare sugar, and the experimental result shows that the scheme of compounding the two fermentation stock solutions and the three kinds of rare sugar has the most obvious effect. Wherein, the weight of the mice in the group of sample 5 is reduced the most, and the weight is close to that of the normal mice, which shows that the ferment of sample 5 has the best effect of reducing the weight of the mice.

TABLE 3 weight condition of each group of mice after 6 weeks of administration

Note: # indicates significance compared to the blank control group and # indicates significance compared to the model control group.

2.2 weight changes in mouse tissues

The change of the tissue weight of the mouse is shown in table 4, and the liver, perirenal fat and epididymal fat of the model group are obviously increased compared with those of the blank control group, which indicates that the model building is successful. Compared with the model group, the liver weight of the mice in the sample 4 group is obviously reduced, and the liver weight of the mice in the sample 5 group is extremely obviously reduced. Compared with a model group, the liver weights of mice in the groups of the sample 1, the sample 2 and the sample 6-10 are reduced, but no obvious difference exists, and the results of the control sample 3-5 show that five raw materials, namely fructo-oligosaccharide, galacto-oligosaccharide, trehalose, aloe fermentation stock solution and comprehensive fruit and vegetable fermentation stock solution, have influence on the liver weight of the mice and play a necessary role in the product; meanwhile, the effect of the combination of the two fermentation stock solutions and the three rare sugars on the reduction of the weight of the liver is obvious.

Compared with the model group, the epididymal fat of the mice of the sample 2, the sample 6 and the sample 7 has no significant difference, and one or two fermentation stock solutions are lacked in the raw materials of the three samples, so that the results show that both the aloe fermentation stock solution and the comprehensive fruit and vegetable fermentation stock solution have influence on the epididymal fat of the mice. Compared with the model group, the epididymal fat of the mice in the sample 1 group has no obvious difference, and the raw materials of the sample 1 group do not contain rare sugar, which shows that the rare sugar plays a main role in reducing the epididymal fat of the mice. Meanwhile, compared with the model group, the sample 2 group containing only rare sugar in the raw materials has lower epididymal fat reduction degree than the sample 3-10 group, which shows that the compounding of the fermentation stock solution and the rare sugar has the synergistic effect. The raw materials of the 8-10 groups of samples respectively contain two kinds of rare sugar and two kinds of fermentation stock solutions, the epididymis fat of mice is reduced remarkably, but the reduction degree is lower than that of the 3-5 groups of samples, and the reduction effect of the compounding of the three kinds of oligosaccharide and the two kinds of fermentation stock solutions on the epididymis fat of the mice is most remarkable. Among them, the fat-reducing effect of epididymis was the best in sample 5.

Compared with the model group, the perirenal fat of the mice of the sample 2 and the sample 6 has no obvious difference, while the perirenal fat of the mice of the sample 7 is obviously reduced, which shows that the aloe fermentation stock solution and the combination of the three kinds of rare sugar have the synergistic effect and can obviously reduce the perirenal fat of the mice. Compared with the model group, the perirenal fat of the mice in the sample 1 group has no significant difference, which shows that only two fermentation stock solutions have insignificant effect on reducing the perirenal fat of the mice. Meanwhile, compared with the model group, the perirenal fat reduction degree of the sample 2 group containing only rare sugar in the raw materials is lower than that of the sample 3-10 group, which shows that the compounding of the fermentation stock solution and the rare sugar has the synergistic effect. The perirenal fat of mice in the 8-10 groups of samples is obviously reduced, the raw materials respectively contain two kinds of rare sugar, but the reduction degree is lower than that of the 3-5 groups of samples, and the reduction effect of the combination of the three kinds of oligosaccharide and the two kinds of rare sugar on the perirenal fat of the mice is most obvious. Among them, the sample 5 group had the best effect of reducing perirenal fat.

TABLE 4 mean weights of mouse tissues

Note: # indicates significance compared to the blank control group and # indicates significance compared to the model control group.

2.3 analysis of the species diversity of the intestinal flora

The abundance and diversity of microbial communities can be reflected by a diversity analysis (α diversity) of a single sample. Judging the abundance of the flora by using a Chao index, wherein the larger the Chao index is, the higher the abundance of the flora is. The diversity of the flora was estimated by Shannon and Simpson indices. The larger the Shannon index, the higher the community diversity. The larger the Simpson index, the lower the community diversity.

The alpha diversity index of the intestinal flora of the mice of each group of samples is shown in table 5, and the results show that the species abundance degree Chao index of the model group is obviously reduced compared with that of a blank control group, the species diversity Shannon index is obviously reduced compared with that of a control group, and the Simpson index is obviously increased compared with that of the control group, which indicates that the species abundance degree and species diversity of the intestinal flora of the obese mice are obviously reduced compared with that of the control group. Compared with the model group, the Chao indexes show a rising trend after the ferment samples 1-10 are administered; except for the sample 1 and 2 groups, the Shannon indexes of the rest groups show a rising trend, wherein the sample 5 group is greatly increased compared with the model group; the Simpson index is reduced, the sample 9 group is obviously reduced compared with the model group, the sample 3 group is obviously reduced compared with the model group, and the samples 4 and 5 group are extremely obviously reduced compared with the model group (p is less than 0.01 in the sample 4 group and p is less than 0.001 in the sample 5 group), which indicates that the ferment increases the abundance of floras and increases the diversity of mouse intestinal floras.

TABLE 5 mouse intestinal flora alpha diversity index

Note: # indicates significance compared to the blank control group and # indicates significance compared to the model control group.

In conclusion, the ferment disclosed by the invention has obvious effects of preventing obesity and regulating intestinal flora, can reduce the weight gain of mice, reduce the weight of liver, perirenal fat and epididymal fat, and can increase the diversity of the intestinal flora of the mice.

Claims (8)

1. The ferment for preventing obesity and regulating intestinal flora is characterized by being prepared by fermenting the following raw materials: 20-40 parts of aloe fermentation stock solution, 40-60 parts of comprehensive fruit and vegetable fermentation stock solution, 1-15 parts of fructo-oligosaccharide, 1-15 parts of galacto-oligosaccharide and 1-10 parts of trehalose.

2. The ferment for preventing obesity and regulating intestinal flora according to claim 1, wherein the ferment is prepared by fermenting the following raw materials: 15-25 parts of aloe fermentation stock solution, 45-55 parts of comprehensive fruit and vegetable fermentation stock solution, 3-12 parts of fructo-oligosaccharide, 3-12 parts of galacto-oligosaccharide and 2-8 parts of trehalose.

3. The ferment for preventing obesity and regulating intestinal flora according to claim 1 or 2, wherein the aloe is at least one of aloe vera, aloe arborescens and aloe vera.

4. The ferment for preventing obesity and regulating intestinal flora according to claim 3, wherein the aloe is aloe gel, and the aloe gel is prepared by using the aloe leaves as a raw material and performing steps of leaching quinone, cleaning, peeling, blanching and sterilizing.

5. The ferment for preventing obesity and regulating intestinal flora according to claim 1 or 2, wherein the fruit and vegetable raw materials of the integrated fruit and vegetable fermentation stock solution comprise: lemon, green plum, papaya, kiwi fruit, mulberry, medlar, red date and/or rose.

6. The ferment for preventing obesity and regulating intestinal flora according to claim 5, wherein the fruit and vegetable raw materials of the integrated fruit and vegetable fermentation stock solution further comprise: pineapple, plum, grape, dragon fruit, apple, hawthorn, green tea, okra, corn, mushroom, prune, noni, dark plum, citrus, grapefruit, and/or blueberry.

7. The method for preparing ferment for preventing obesity and regulating intestinal flora according to any one of claims 1 to 6, wherein the method comprises the following steps:

fermentation in the first stage: fermenting the aloe and the fruit and vegetable raw materials at normal temperature for 3 months to 2 years respectively;

and (3) second-stage fermentation: mixing the aloe and fruit and vegetable fermentation stock solution obtained in the first stage fermentation with the rare sugar according to the weight ratio, and fermenting by adopting lactobacillus plantarum and/or saccharomycetes at the fermentation temperature of 25-36 ℃ for 7-30 days.

8. Use of the ferment for preventing obesity and regulating intestinal flora according to any one of claims 1 to 7 in the preparation of a medicament or food for preventing obesity and regulating intestinal flora function.