KR20220036434A - Cosmetic composition containing fermented water-soluble chitosan having low molecular weight - Google Patents

Abstract

The present invention relates to a cosmetic composition. The cosmetic composition of the present invention contains fermented water-soluble chitosan having low molecular weight and a component derived from a specific natural substance, and thus has a high adsorption capacity for heavy metals and fine dust. Furthermore, the cosmetic composition exhibits highly effective antibacterial and anti-inflammatory effects, and has the advantage of being highly effective in regenerating and moisturizing the skin, and ameliorating skin wrinkles.

Description

Cosmetic composition containing fermented water-soluble chitosan having low molecular weight

The present invention relates to a cosmetic composition comprising fermented water-soluble chitosan having a low molecular weight.

The skin is a part of the body that is directly exposed to the external environment, and it not only acts as a protective film to protect important organs of our body, but also regulates water evaporation and protects the body from external infections. However, even skin that blocks the penetration of viruses from the outside causes skin irritation and inflammatory reactions such as erythema, edema, and itching when exposed to excessive ultraviolet rays or pollutants. be injured by Yellow sand is a complex of organic and inorganic substances, and its physical properties and components vary greatly depending on the time and place of occurrence, and it also contains metal components that can have a biological effect, so there is a need to completely remove it from the skin.

In addition, as human skin ages, it undergoes changes due to various internal and external factors. That is, internally, the secretion of various hormones that control metabolism is reduced, the function and activity of immune cells is lowered, and the biosynthesis of immune proteins and biological constituent proteins necessary for the living body is reduced. As the environmental pollution worsens, free radicals and active free radicals increase, so that the thickness of the skin decreases, wrinkles increase, elasticity decreases, and the complexion of the skin becomes dull and poor, and skin troubles occur frequently. In addition, it causes various changes such as increased spots, freckles, and age spots.

Accordingly, there is a need for completely removing various pollutants as well as yellow dust, fine dust, etc., as well as controlling moisture of the skin or slowing down aging.

On the other hand, chitosan is contained in crustaceans, which is a material that is processed to make chitin easily absorbed by the human body. It has almost the same structure as cellulose, but it is of animal origin and has an amino group, so it is not soluble in water and is soluble in acid. In addition, chitin contained in crab, crayfish and shrimp shells is deacetylated under strong alkaline conditions to contain more than 80% of the constituent units of β-(1,4)-2-amino-D-glucose, and is harmless to the human body. It is known as a biodegradable natural polymer material that can form a film.

In particular, chitosan having a molecular weight of 20,000 to 100,000 is known to have a strong physiological activity, so it is being used variously in the health food field, food and beverage field, health hygiene field, medicine field, and the like. However, efforts to apply chitosan to skin products due to its excellent antibacterial action and emulsion stability have been developed. it is not the case.

Republic of Korea Patent Publication No. 10-2004-0080717

Accordingly, it is an object of the present invention to provide a cosmetic composition having excellent effects of not only adsorbing heavy metals and fine dust, but also antibacterial/anti-inflammatory, skin regeneration, moisturizing, and wrinkle improvement.

As a means for solving the above problems,

The present invention as an embodiment, water-soluble chitosan having an average molecular weight of 2,000 to 10,000; And it provides a cosmetic composition comprising a component derived from a natural substance.

In this case, the content of the water-soluble chitosan may be 0.01 to 5 parts by weight based on 100 parts by weight of the total cosmetic composition.

In addition, the water-soluble chitosan may be decomposed by acetic acid or may be fermented by an enzyme.

In addition, the natural substance-derived ingredients include licorice root extract, ginger extract, omija extract, yellow lily extract, green tea extract, grapefruit peel oil, propolis wax, betaine, allantoin, panthenol, tocopheryl acetate, asiaticoside, vinegar and adenosine. It may be derived from two or more kinds of natural substances selected from the group consisting of.

Here, the content of the natural substance-derived component may be 0.01 to 10 parts by weight based on 100 parts by weight of the total cosmetic composition.

In addition, the cosmetic composition may further include an additive.

Furthermore, the cosmetic composition is based on 100 parts by weight of the total,

0.01 to 2.0 parts by weight of chitosan;

Ingredients derived from natural substances including licorice root extract, ginger extract, omija extract, yellow lily extract, green tea extract, grapefruit peel oil, propolis wax, betaine, allantoin, panthenol, tocopheryl acetate, asiaticoside, vinegar and adenosine 0.01 to 5 parts by weight; and

Glycerin, butylene glycol, 1,2-hexanediol, hydroxyethyl cellulose, polyglyceryl-10 laurate, polyglyceryl-10 myristate, caprylic glycol, ethylhexyl glycerin and disodium EDI 12 to 18 parts by weight of an additive containing A;

The water-soluble chitosan may be decomposed by acetic acid to have an average molecular weight of 2,000 to 10,000.

The cosmetic composition according to the present invention contains low-molecular-weight fermented water-soluble chitosan and ingredients derived from specific natural substances, which not only have excellent adsorption capacity for heavy metals and fine dust, but also have excellent effects such as antibacterial/anti-inflammatory, skin regeneration, moisturizing, and wrinkle improvement. There is an advantage.

The above effects and additional effects will be described in detail below.

Prior to describing the present invention in detail below, it is to be understood that the terminology used herein is for the purpose of describing specific embodiments and is not intended to limit the scope of the present invention, which is limited only by the appended claims. shall. All technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the art, unless otherwise stated.

In the present invention, the use of “or” or “and” means “and/or” unless otherwise indicated. Moreover, the use of the term “accompanying” as well as other forms such as “accompanying” and “accompanying” is not limiting. Section headings used in the present invention are for organizational purposes only and should not be construed as limiting the subject matter described.

Throughout this specification and claims, unless stated otherwise, the term comprise, comprises, comprising is meant to include the stated object, step or group of objects, and steps, and any other object. It is not used in the sense of excluding a step or a group of objects or groups of steps.

Hereinafter, the present invention will be described in more detail.

cosmetic composition

The present invention, in one embodiment, water-soluble chitosan; And it provides a cosmetic composition comprising a natural extract.

The cosmetic composition according to the present invention contains water-soluble chitosan having an average molecular weight in a specific range and ingredients derived from specific natural substances, and has excellent adsorption ability of heavy metals and fine dust, as well as antibacterial/anti-inflammatory, skin regeneration, moisturizing, wrinkle improvement, etc. It has the advantage of being effective.

Here, the chitosan is a low molecular weight water-soluble chitosan, and may have an average molecular weight of 2,000 to 10,000, specifically 2,000 to 6,000; 2,000 to 5,000; 2,000 to 4,500; 2,000 to 4,000; 3,000 to 6,000; 4,000 to 6,000; or 2,3000 to 2,800.

The present invention can maximize the skin moisturizing effect by improving the skin absorption rate of chitosan in the composition by adjusting the average molecular weight of the water-soluble chitosan within the above range.

In addition, the content of the water-soluble chitosan may be 0.01 to 5.0 parts by weight based on 100 parts by weight of the total cosmetic composition, specifically 0.01 to 2.0 parts by weight, 0.01 to 1.0 parts by weight, 0.01 to 0.5 parts by weight, 0.01 to 0.1 parts by weight. , 0.02 to 0.08 parts by weight or 0.04 to 0.07 parts by weight.

Generally, high molecular weight water-soluble chitosan is harmless to the human body, but low molecular weight water-soluble chitosan has a high skin absorption rate and may cause skin irritation and/or skin troubles. However, the present invention can realize excellent skin regeneration ability while preventing skin irritation and/or skin troubles by adjusting the low molecular weight water-soluble chitosan in the cosmetic composition to the above range.

In addition, the water-soluble chitosan may be one in which the molecular weight is controlled to have a low molecular weight by treating the water-soluble chitosan having a high molecular weight with acetic acid. Specifically, the water-soluble chitosan is dissolved in purified water to 5±1% by weight, and acetic acid is added so that the pH of the solution in which the water-soluble chitosan is dissolved becomes 5.0±0.3, followed by stirring at 40-80° C. for 4-8 hours. It may be decomposed into a small molecular weight, and thus the decomposed water-soluble chitosan may contain some acetic acid.

Here, the amount of acetic acid added may be 20-40 parts by weight when the concentration of acetic acid is 1.5-2.0% by weight based on 1 part by weight of water-soluble chitosan; When the concentration of acetic acid is 100% by weight, it may be 0.3 to 0.8 parts by weight. In addition, 5 to 20% by weight of the decomposed water-soluble chitosan may be acetic acid, and specifically, 8 to 15% by weight may be acetic acid.

As an example, low-molecular-weight water-soluble chitosan is dissolved in purified water to be 5±0.1 wt%, and the pH of the solution in which water-soluble chitosan is dissolved is 5.0±0.3 with respect to 1 part by weight of water-soluble chitosan acetic acid at a concentration of 1.75 wt% After adding 30±0.5 parts by weight, it can be prepared by stirring at 40 to 65° C. for 4 to 6 hours, and the cosmetic composition comprising low molecular weight water-soluble chitosan (containing 8 to 15% by weight of acetic acid) prepared in this way is acetic acid It may be contained in an amount of 0.005 to 0.009 parts by weight based on 100 parts by weight of the cosmetic composition.

The present invention is a free amino group bonded to the 2-position carbon of water-soluble chitosan (-NH ₂ ) and a hydroxyl group bonded to the 3-position and/or 6-position carbon of the water-soluble chitosan by lowering the molecular weight of the water-soluble chitosan in the same manner as described above (- OH) damage can be minimized.

Here, the free amino group (-NH ₂ ) is cationized (-NH ₃ ⁺ ) and binds to the cell membrane of the microorganism having (-) ionicity to inhibit the proliferation of microorganisms, thereby imparting an antibacterial effect; Together with the hydroxyl group (-OH), it acts as a ligand for heavy metals or interacts with fine dust to achieve the effect of adsorption, so by minimizing their damage, the cosmetic composition has an antibacterial effect and adsorption ability of heavy metals and/or fine dust. can improve

Furthermore, the low molecular weight water-soluble chitosan may be fermented by an enzyme. Specifically, after the water-soluble chitosan is low molecular weight by acetic acid, 0.1 ~ 5.0 U / g of the enzyme chitosanase is added to the low molecular weight aqueous chitosan solution and 2 ~ 10 at 30 ~ 40 ℃ It may be fermented by stirring for a period of time.

Here, the chitosanaase may be used without particular limitation as long as it is commonly used in the art.

The present invention can significantly improve skin absorption by fermenting low molecular weight water-soluble chitosan as described above.

Furthermore, the natural substance-derived component may refer to an extract, a fraction, or a compound obtained from a natural substance. Specifically, the natural substance-derived component may include extracts of licorice root, ginger, omija, yellow lily, and green tea, and may include oil obtained from fruit peels such as grapefruit peel, propolis wax derived from honey, etc. . In addition, the natural substance-derived component may include a compound isolated from a natural substance, such as betaine, allantoin, panthenol, tocopheryl acetate, asiaticoside, adenosine, etc. Artificially fermented vinegar (vinegar) and the like may be included. Here, the extract may be purchased and used commercially, and in some cases, it may be prepared by a method commonly applied in the art, for example, in the case of an extract, compression extraction method, solvent extraction method, etc. However, the present invention is not limited thereto.

In addition, the content of the natural substance-derived component may be 0.01 to 10 parts by weight based on 100 parts by weight of the total cosmetic composition, specifically 0.01 to 5 parts by weight, 0.01 to 3 parts by weight, 0.5 to 4 parts by weight, 0.5 to 2 parts by weight. It may be in an amount of 0.8 to 1.8 parts by weight, or 1.0 to 1.5 parts by weight.

As an example, when extracts obtained from natural substances, propolis wax and grapefruit peel oil are included, it may be included in an amount of 0.01 to 0.08 parts by weight based on 100 parts by weight of the total cosmetic composition; In the case of a compound isolated from a natural substance, it may be included in an amount of 1.1 to 1.6 parts by weight based on 100 parts by weight of the total cosmetic composition; In the case of a fermented product such as a natural substance fermented vinegar, it may be included in an amount of 0.02 to 0.07 parts by weight based on 100 parts by weight of the total cosmetic composition.

On the other hand, the cosmetic composition may further include additives commonly used in the art in addition to the low molecular weight water-soluble chitosan and ingredients derived from natural substances. These additives include alcohols, antioxidants, emulsion stabilizers/surfactants, solubilizers, vitamins, preservatives, humectants, moisturizers, viscosity modifiers, sunscreens, skin/hair conditioning agents, sterilization preservatives, hair fixatives, pH adjusters , sequestering agents, modifiers, excipients, carriers, pigments, fragrances, solvents, etc., and specific compounds or compositions that can be used as additives are already known in the art, so those skilled in the art can select and use the appropriate compound or composition can

For example, as the surfactant, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether sulfate, alkylamidobeta Phosphorus, fatty alcohol, fatty acid glyceride, fatty acid diethanol amide, vegetable oil, linolin derivative or ethoxylated glycerol fatty acid ester, hydroxyethyl cellulose, etc. may be used.

In addition, as the skin/hair conditioning agent, hydroxyethyl cellulose, polyglyceryl-10 laurate, polyglyceryl-10 myristate, caprylic glycol, ethylhexylglycerin, and the like may be used.

In addition, as the sequestering agent, disodium EDTA may be used.

In addition, examples of the alcohol include water-soluble polyhydric alcohols such as higher alcohols, propylene glycol, 1,3-butylene glycol, glycerin, 1,2-hexanediol, sorbitol, and polyethylene glycol, and the oil is Avogard. Oil, palm oil, tallow, jojoba oil, etc. are mentioned.

In addition, examples of the preservative include ethylparaben and butylparaben, examples of the moisturizing agent include hyaluronic acid, chondroitin sulfate, and pyrrolidone carboxylate, and the diluent includes ethanol and isopropanol.

Here, the content of the additive may be 5 to 20 parts by weight based on 100 parts by weight of the total cosmetic composition, specifically 5 to 18 parts by weight, 10 to 20 parts by weight, 15 to 20 parts by weight, 12 to 17 parts by weight, or 14 to 16 parts by weight.

Furthermore, the cosmetic composition according to the present invention may further include purified water together with low molecular weight water-soluble chitosan, natural substance-derived ingredients and additives, wherein the purified water acts on the cosmetic composition as a skin conditioning agent and solvent, and the content is It may be the same as the remaining amount excluding the chitosan, ingredients derived from natural substances and additives contained in the composition.

As an example, the cosmetic composition according to the present invention is based on 100 parts by weight of the total,

0.01 to 2.0 parts by weight of chitosan;

Ingredients derived from natural substances including licorice root extract, ginger extract, omija extract, yellow lily extract, green tea extract, grapefruit peel oil, propolis wax, betaine, allantoin, panthenol, tocopheryl acetate, asiaticoside, vinegar and adenosine 0.01 to 5 parts by weight; and

Glycerin, butylene glycol, 1,2-hexanediol, hydroxyethyl cellulose, polyglyceryl-10 laurate, polyglyceryl-10 myristate, caprylic glycol, ethylhexyl glycerin and disodium EDI 12 to 18 parts by weight of an additive containing A; and

Contains 75 to 87.98 parts by weight of purified water,

The chitosan may be decomposed by acetic acid to have an average molecular weight of 2,000 to 10,000.

The present invention can maximize the skin regeneration, wrinkle improvement, skin soothing effect, etc. with a small amount while preventing economic degradation problems due to excessive use by adjusting the content of the natural extract to the above range.

functional cosmetics

In addition, the present invention, in one embodiment, provides a functional cosmetic comprising the cosmetic composition of the present invention described above.

Functional cosmetics according to the present invention include a cosmetic composition containing low-molecular-weight fermented water-soluble chitosan and components derived from specific natural substances, and not only have excellent adsorption capacity for heavy metals and fine dust, but also antibacterial/anti-inflammatory, skin regeneration, moisturizing, and wrinkle improvement Since it has excellent effects such as, it can be easily used as a functional cosmetic.

Here, as the functional cosmetics, as skin and body care cosmetics containing the cosmetic composition of the present invention, lotions, skins, essences, serums, ampoules, emulsions, lotions, nourishing creams, massage creams, moisture creams, hand creams, soaps, Tonics and body essences, body oils, body cleansers, facial cleansers, creams (oil-in-water, water-in-oil, multiphase), solutions, suspensions (anhydrous and water-based), anhydrous products (oil and glycol-based), gels, powders, It can be prepared in various formulations such as emulsion foundation, wax foundation, patches and sprays, and the cosmetic composition is included in an amount of 0.05 to 100 wt% with respect to the total cosmetics, and in the case of an ampoule formulation, it can be made of 100% of the cosmetic composition there is.

The functional cosmetic may include an acceptable carrier in the skin cosmetic preparation in addition to the cosmetic composition of the present invention.

Examples of the carrier include alcohol, oil, surfactant, fatty acid, silicone oil, preservative, humectant, humectant, viscosity modifier, emulsion stabilizer, sunscreen, color developer, fragrance, diluent, and the like.

Specific compounds or compositions that can be used as the alcohol, oil, surfactant, fatty acid, silicone oil, preservative, wetting agent, humectant, viscosity modifier, emulsifier, stabilizer, sunscreen, color developer, fragrance, diluent, etc. are already known in the art. Therefore, those skilled in the art can select and use an appropriate compound or composition.

Here, to exemplify some examples, examples of the alcohol include water-soluble polyhydric alcohols such as higher alcohols, propylene glycol, 1,3-butylene glycol, glycerin, sorbitol, and polyethylene glycol, and examples of the oils include avogard oil, palm oil, and tallow , jojoba oil, etc., ethylparaben, butylparaben, etc. are mentioned as preservatives, hyaluronic acid, chondroitin sulfate, pyrrolidone carboxylate etc. are mentioned as a moisturizing agent, and ethanol, isopropanol as a diluent. and the like.

More specifically, when the formulation of the functional cosmetic is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide as a carrier component etc. may be used.

In addition, when the formulation is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane/ propellants such as butane or dimethyl ether.

In addition, when the formulation is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol, or fatty acid ester of sorbitan.

Further, when the formulation is a suspension, as a carrier component, water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline cellulose , aluminum metahydroxide, bentonite, agar or tracanth may be used.

In addition, when the formulation is a surfactant-containing cleansing agent, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide as carrier components Ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanol amide, vegetable oil, linolin derivative or ethoxylated glycerol fatty acid ester and the like may be used.

In addition, in the case of a soap comprising the cosmetic composition according to an embodiment of the present invention, it may be prepared by including the cosmetic composition in the soap base, and may be prepared by including a skin moisturizer, an emulsifier, a light water softener, etc. as an auxiliary additive. .

As the soap base, vegetable oils such as palm oil, palm oil, soybean oil, perm oil, olive oil, and palm kernels or animal fats such as tallow, lard, brisket, and fish oil may be used, and as the skin moisturizer, glycerin, erythritol, polyethylene glycol , propylene glycol, butylene glycol, pentylene glycol, hexyl glycol, isopropyl myristate, silicone derivatives, aloe vera, sorbitol, etc. can be used, and as the emulsifier, natural oils, wax fatty alcohols, hydrocarbons, natural plant extracts, etc. may be used, and as the water softener, tetrasodium EDITA may be used.

In addition, the soap composition of the present invention may additionally include an antibacterial agent, a dispersant, a foam inhibitor, a solvent, a scale inhibitor, a corrosion inhibitor, a fragrance, a colorant, a sequestering agent, an antioxidant, and a preservative as auxiliary additives.

Hereinafter, the present invention will be described in detail by way of preparation examples and examples.

However, the following Preparation Examples and Examples only illustrate the present invention, the content of the present invention is not limited to the following Preparation Examples and Examples.

Preparation Example 1. Preparation of low molecular weight water-soluble chitosan

Chitosan having an average molecular weight of 20,000 to 22,000 was added to purified water so that the final concentration was 5±0.2 wt%, and acetic acid (concentration: 1.75 wt%) was added so that the final pH was 5.0. Then, the reaction solution was heated to adjust the temperature of the solution to 40±1° C., and stirred for 4 to 6 hours to reduce the molecular weight of water-soluble chitosan.

Then, the low molecular weight water-soluble chitosan was passed through a silver ion exchange resin to recover the eluate, purified and concentrated, and then the concentrate was freeze-dried to obtain a low molecular weight water-soluble chitosan powder.

Preparation Example 2. Preparation of fermented low molecular weight water-soluble chitosan

Chitosan having an average molecular weight of 20,000 to 22,000 was added to purified water to have a final concentration of 5±0.2 wt%, and acetic acid (concentration: 1.75 wt%) was added so that the final pH was 5.0. Then, the reaction solution was heated to adjust the temperature of the solution to 40±1° C., and stirred for 4 to 6 hours to prepare low molecular weight water-soluble chitosan. Subsequently, 3.0 unit/g of chitosanaase was dissolved in water with respect to the weight of water-soluble chitosan that was added to purified water and added to the previously prepared low-molecular-weight water-soluble chitosan, and stirred at a speed of 10 rpm for 5 hours to reduce Molecular weight water-soluble chitosan was fermented.

Then, the fermented low-molecular-weight water-soluble chitosan was passed through a silver ion exchange resin to recover the eluate, purified and concentrated, and then the concentrate was freeze-dried to obtain a fermented low-molecular-weight water-soluble chitosan powder.

Examples 1-2 and Comparative Examples 1-6. Preparation of cosmetic composition

As shown in Table 1 below, water-soluble chitosan obtained in Preparation Examples 1 and 2, ingredients derived from natural substances, additives and purified water were weighed and mixed to prepare a cosmetic composition. At this time, the mixing amount of each raw material used as the ingredient derived from the natural substance and the additive is as shown in Table 2 below.

Unit: parts by weight Example comparative example One 2 One 2 3 4 5 6 chitosan water soluble chitosan
(Production Example 1) 0.04~0.08 - - 0.001 6 - - 0.04~0.08 Fermented Water Soluble Chitosan
(Production Example 2) - 0.04~0.08 - - - 0.001 6 - Ingredients derived from natural substances 1.0~2.0 - additive 14-16 - Purified water Remaining amount (to 100g)

Mixing amount [g] natural
matter
origin
ingredient Licorice Root Extract 0.00005~0.0002 ginger extract 0.00005~0.0002 Schisandra extract 0.00005~0.0002 Yellow Lily Extract 0.00005~0.0002 green tea extract 0.00005~0.0002 propolis wax 0.000001 to 0.00001 grapefruit peel oil 0.01~0.1 Vinegar 0.01~0.1 betaine 0.5~2 allantoin 0.05~0.5 panthenol 0.05~0.5 tocopheryl acetate 0.01~0.1 Asiaticoside 0.0000005 to 0.00001 adenosine 0.01~0.1 additive Hydroxyethyl Cellulose 0.1~1 Polyglyceryl-10 Laurate 0.05~0.5 Polyglyceryl-10 myristate 0.05~0.5 caprylic glycol 0.01~0.5 Ethylhexylglycerin 0.05~0.5 Disodium EDIT 0.01~0.1 1,2-Hexanediol 1-3 Butylene Glycol 3-8 glycerin 5-8

Experimental Example 1. Measurement of molecular weight of water-soluble chitosan

Molecular weights were measured for the water-soluble chitosan powder prepared in Preparation Examples 1 and 2 and the chitosan (control group) having an average molecular weight of 20,000 to 22,000 used in Preparation Example 1.

Specifically, the results measured by measuring the average molecular weight of chitosan powder using gel permeation chromatography (GPC) are shown in Table 3 below, and the measurement conditions are as follows:

- Analysis System: Waters HPLC-GPC

- Column: Ultrahydrogel 250 GPC column (Mw 1,000~80,000)

- Solvent: 0.1 M NaCl acetic acid solution

- Flow rate: 0.3 mL/min

- Detector: RI detector

- Injection volume: 15 μl/time

- Standard: dextran molecular weight 8,000, 11,500, 40,500, 69,500

average molecular weight control 21,000±1,000 Chitosan of Preparation Example 1 2,800±140 Chitosan of Preparation Example 2 2,650±130

Referring to Table 3, it was confirmed that the low molecular weight water-soluble chitosan according to the present invention was decomposed by acetic acid to have a molecular weight of about 2,500 to 3,000. From these results, it can be seen that the water-soluble chitosan according to the present invention has an average molecular weight of 2,000 to 10,000, more specifically, 2,000 to 6,000 by acetic acid.

Experimental Example 2. Performance evaluation of cosmetic compositions

The following experiments were performed to evaluate the performance of the cosmetic composition according to the present invention in adsorption of heavy metals and fine dust, anti-inflammatory, moisturizing, skin irritation and soothing, and wrinkle improvement.

A) Adsorption capacity of heavy metals and fine dust

Prepare a dispersion in which lead, cadmium, copper, arsenic, zinc, and fine dust (PM2.5) are dispersed by 100 ppm each, mix with 10 mL of the cosmetic composition prepared in Examples and Comparative Examples, and then stir for 1 hour After that, it was filtered. Then, the residual heavy metal and fine dust content of the filtrate was measured by atomic absorption spectroscopy, and the heavy metal and fine dust adsorption rate of the cosmetic composition was calculated from the measured residual heavy metal and fine dust content. The results are shown in Table 4 below.

lead cadmium Copper arsenic zinc fine dust Example 1 99.5% 96.7% 98.1% 97.2% 98.9% 95.1% Example 2 98.9% 97.3% 97.8% 98.5% 95.1% 99.0% Comparative Example 1 61.8% 67.2% 45.4% 55.7% 58.5% 49.1% Comparative Example 2 71.7% 78.9% 61.5% 69.6% 70.8% 65.3% Comparative Example 3 84.3% 82.5% 79.1% 75.8% 88.2% 89.6% Comparative Example 4 75.2% 81.2% 64.1% 73.2% 71.5% 67.8% Comparative Example 5 83.9% 82.7% 80.3% 77.4% 88.7% 88.8% Comparative Example 6 85.5% 83.3% 87.9% 86.4% 89.3% 88.7%

b) Anti-inflammatory performance

First, RAW264.7 cells were aliquoted in a 96 well plate at 2 × 106 cells/ml and the cells were cultured for 18 hours, and then each of the cosmetic compositions prepared in Examples and Comparative Examples were treated at 100 μg/ml for 30 minutes, and , and incubated for 24 hours. After incubation, LPS was treated at a concentration of 1 μg/ml, and cultured in an incubator at 37° C. and 5% CO 2 conditions for 20 hours. Mix 100 μl of the cultured cell culture solution and 100 μl of Griess reagent (1% sulfanilamide in 5% phosphoric acid and 1% α-naphthylamide in H20), react at room temperature for 5 minutes, and then use an ELISA microplatereader (Tecan, USA) at 540 nm. Absorbance was measured. After measuring the absorbance with sodium nitrite (NaNO ₂ ) to obtain a standard curve, the concentration (%) of N0 was calculated from the measured absorbance, and LPS and purified water were used as positive and negative controls, respectively. The measured results are shown in Table 5 below.

NO inhibitory ability Standard Deviation positive control 100.0% 5.3% negative control 0.00% - Example 1 68.7% 8.10% Example 2 65.3% 7.3% Comparative Example 1 35.1% 7.1% Comparative Example 2 41.9% 6.9% Comparative Example 3 63.7% 7.4% Comparative Example 4 40.8% 6.5% Comparative Example 5 67.8% 7.6%

C) Moisturizing performance

For 18 experimenters (females aged 20-35 years), the cosmetic compositions of Examples and Comparative Examples were applied to the faces of each of two persons twice a day for 4 consecutive weeks. After completion of the experiment, the moisture content of the facial skin was measured with a corneometer CM820 (Courage & Khazaka, Cologne, Germany) and a tewameter TM210 (Courage & Khazaka, Cologne, Germany) to calculate the moisture content, and the results are shown in Table 6.

moisture content Example 1 72.3% Example 2 76.1% Comparative Example 1 55.2% Comparative Example 2 58.7% Comparative Example 3 64.7% Comparative Example 4 57.9% Comparative Example 5 67.6%

D) Skin hypoallergenic performance

A patch containing the cosmetic composition prepared according to the above Examples and Comparative Examples as an active ingredient was prepared, and the human skin primary stimulation test was performed using the patch for 48 hours.

Specifically, a patch (2×4 cm) containing the cosmetic composition prepared according to Examples and Comparative Examples was prepared, and the patch was removed after occluded patching for 6 hours on clinical subjects or the like. Then, when 24 hours have elapsed, the degree of skin irritation was evaluated by observing the part to which the patch was attached. At this time, the clinical subjects were selected from a total of 20 women and men in their 10s and 50s, 10 each. In this case, purified water was used as a control for the cosmetic composition, and the evaluation criteria for skin irritation were as follows, and the results are shown in Table 7:

No stimulation: sensitization value 0.00 or more and less than 0.15

Mild irritation: sensitization value 0.15 or more and less than 0.70

Moderate stimulation: sensitization value 0.70 or more and less than 1.00

Strong stimulus: sensitization value 1.00 or higher

stimulus level Judgment control 2.81% strong stimulus Example 1 0.11% non-irritant Example 2 0.08% non-irritant Comparative Example 1 0.75% central stimulus Comparative Example 2 0.33% mild stimulation Comparative Example 3 0.13% non-irritant Comparative Example 4 0.28% mild stimulation Comparative Example 5 0.10% non-irritant

E) Wrinkle improvement performance

The wrinkle improvement effect of the cosmetic compositions prepared in Examples and Comparative Examples was tested by measuring the proliferation ability of human fibroblasts, which are collagen-forming cells in the dermal layer of the skin.

Specifically, human fibroblasts cultured in DMEM medium were seeded at 1×10 ⁴ cells per well in a 96-well-plate. Here, ascorbic acid, which is known to have a skin wrinkle improvement effect, as a positive control group, and the cosmetic compositions of Examples and Comparative Examples were added as an experimental group, respectively, and cultured for 72 hours. After culturing, the Easytox kit reagent for staining live cells was treated. After 1 hour, the absorbance was measured at 450 nm using an ELISA reader to calculate the skin cell proliferation (eg, the higher the absorbance, the better the proliferation), and the results are shown in Table 8 below.

Absorbance at 450 nm positive control 59.7% Example 1 63.1% Example 2 65.7% Comparative Example 1 48.2% Comparative Example 2 49.6% Comparative Example 3 60.8% Comparative Example 4 48.9% Comparative Example 5 61.5%

As shown in Tables 4 to 8 above, the cosmetic composition according to the present invention contains fermented water-soluble chitosan and a component derived from a specific natural material, so that it has excellent adsorption ability for heavy metals and fine dust, as well as antibacterial/anti-inflammatory, hypoallergenic, It has the advantage of excellent effects such as moisturizing and wrinkle improvement.

Claims (7)

water-soluble chitosan having an average molecular weight of 2,000 to 10,000; and
A cosmetic composition comprising a natural substance-derived ingredient.
According to claim 1,
The content of the water-soluble chitosan is 0.01 to 5 parts by weight based on 100 parts by weight of the total cosmetic composition.
According to claim 1,
The cosmetic composition, characterized in that the water-soluble chitosan is decomposed by acetic acid.
The method of claim 1,
The cosmetic composition, characterized in that the water-soluble chitosan is fermented by an enzyme.
According to claim 1,
The natural substance-derived ingredient consists of licorice root extract, ginger extract, omija extract, yellow lily extract, green tea extract, grapefruit peel oil, propolis wax, betaine, allantoin, panthenol, tocopheryl acetate, asiaticoside, vinegar and adenosine. Derived from two or more natural substances selected from the group,
The content of ingredients derived from natural substances is 0.01 to 10 parts by weight based on 100 parts by weight of the total cosmetic composition.
According to claim 1,
The cosmetic composition further comprises an additive.
Based on 100 parts by weight of the total cosmetic composition,
0.01 to 2.0 parts by weight of chitosan;
Ingredients derived from natural substances, including licorice root extract, ginger extract, omija extract, yellow lily extract, green tea extract, grapefruit peel oil, propolis wax, betaine, allantoin, panthenol, tocopheryl acetate, asiaticoside, vinegar and adenosine 0.01 to 5 parts by weight; and
Glycerin, butylene glycol, 1,2-hexanediol, hydroxyethyl cellulose, polyglyceryl-10 laurate, polyglyceryl-10 myristate, caprylic glycol, ethylhexyl glycerin and disodium EDI 12 to 18 parts by weight of an additive containing A;
The water-soluble chitosan is decomposed by acetic acid, cosmetic composition, characterized in that the average molecular weight is 2,000 to 10,000.