KR20220004090A - 헴-함유 단백질의 생산을 위한 균주 및 방법 - Google Patents

헴-함유 단백질의 생산을 위한 균주 및 방법 Download PDF

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KR20220004090A
KR20220004090A KR1020217037341A KR20217037341A KR20220004090A KR 20220004090 A KR20220004090 A KR 20220004090A KR 1020217037341 A KR1020217037341 A KR 1020217037341A KR 20217037341 A KR20217037341 A KR 20217037341A KR 20220004090 A KR20220004090 A KR 20220004090A
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ala
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mutation
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비스와조이 로이 차우두리
스미타 샨카르
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임파서블 푸즈 인크.
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/795—Porphyrin- or corrin-ring-containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/795—Porphyrin- or corrin-ring-containing peptides
    • C07K14/805—Haemoglobins; Myoglobins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01037—5-Aminolevulinate synthase (2.3.1.37)

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
KR1020217037341A 2019-04-25 2020-04-24 헴-함유 단백질의 생산을 위한 균주 및 방법 Ceased KR20220004090A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962838770P 2019-04-25 2019-04-25
US62/838,770 2019-04-25
PCT/US2020/029946 WO2020219972A1 (en) 2019-04-25 2020-04-24 Strains and methods for production of heme-containing proteins

Publications (1)

Publication Number Publication Date
KR20220004090A true KR20220004090A (ko) 2022-01-11

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KR1020217037341A Ceased KR20220004090A (ko) 2019-04-25 2020-04-24 헴-함유 단백질의 생산을 위한 균주 및 방법

Country Status (13)

Country Link
US (2) US11965167B2 (https=)
EP (1) EP3959311A1 (https=)
JP (2) JP7700046B2 (https=)
KR (1) KR20220004090A (https=)
CN (1) CN114341351A (https=)
AU (1) AU2020263580A1 (https=)
BR (1) BR112021021154A2 (https=)
CA (1) CA3137581A1 (https=)
IL (1) IL287418A (https=)
MX (2) MX2021012851A (https=)
PH (1) PH12021552674A1 (https=)
SG (1) SG11202111693YA (https=)
WO (1) WO2020219972A1 (https=)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20250004435A (ko) 2023-06-29 2025-01-08 인테이크 주식회사 헴 생합성 경로의 미토콘드리아 구획화를 통한 헴 고생산 시스템 및 이의 용도
WO2025183375A1 (ko) * 2024-02-28 2025-09-04 서울과학기술대학교 산학협력단 재조합 플라스미드 벡터를 이용하여 형질전환된 재조합 균주와 이로부터 생성된 heme containing globin 재조합 단백질 및 이의 제조방법

Families Citing this family (12)

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PT3294762T (pt) 2015-05-11 2022-03-21 Impossible Foods Inc Constructos de expressão e métodos de modificar geneticamente levedura metilotrófica
AU2020257270B2 (en) 2019-04-17 2026-01-15 Impossible Foods Inc. Materials and methods for protein production
EP3959311A1 (en) 2019-04-25 2022-03-02 Impossible Foods Inc. Strains and methods for production of heme-containing proteins
US10947552B1 (en) 2020-09-30 2021-03-16 Alpine Roads, Inc. Recombinant fusion proteins for producing milk proteins in plants
AU2021353004A1 (en) 2020-09-30 2023-04-13 Nobell Foods, Inc. Recombinant milk proteins and food compositions comprising the same
US20240200088A1 (en) * 2021-04-13 2024-06-20 Kyomei Ltd. Transgenic Plants Comprising Myoglobin and Methods for Producing Myoglobin in Transgenic Plants
CN116940688A (zh) * 2021-05-10 2023-10-24 韩国科学技术院 具有改善的风味、营养和色泽的食品及其制备方法
AU2022369299A1 (en) 2021-10-19 2024-03-14 Eat Scifi Inc. Plant base/animal cell hybrid meat substitute
KR20240118861A (ko) * 2021-12-16 2024-08-05 임파서블 푸즈 인크. 재조합 단백질의 발현을 증가시키는 유전 인자
CN120302886A (zh) * 2022-10-06 2025-07-11 米克罗食品配料有限公司 多形汉逊酵母来源的组合物及应用
CN116790395B (zh) * 2023-06-28 2024-07-19 江南大学 一种合成高活性血红素类蛋白毕赤酵母底盘菌株的构建及应用
WO2026003081A1 (en) * 2024-06-25 2026-01-02 Danmarks Tekniske Universitet Cells and methods for production of heme

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20250004435A (ko) 2023-06-29 2025-01-08 인테이크 주식회사 헴 생합성 경로의 미토콘드리아 구획화를 통한 헴 고생산 시스템 및 이의 용도
WO2025183375A1 (ko) * 2024-02-28 2025-09-04 서울과학기술대학교 산학협력단 재조합 플라스미드 벡터를 이용하여 형질전환된 재조합 균주와 이로부터 생성된 heme containing globin 재조합 단백질 및 이의 제조방법

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MX2025006371A (es) 2025-07-01
JP2025102779A (ja) 2025-07-08
US20200340000A1 (en) 2020-10-29
CN114341351A (zh) 2022-04-12
BR112021021154A2 (pt) 2021-12-14
EP3959311A1 (en) 2022-03-02
CA3137581A1 (en) 2020-10-29
MX2021012851A (es) 2022-02-10
US11965167B2 (en) 2024-04-23
JP7700046B2 (ja) 2025-06-30
WO2020219972A1 (en) 2020-10-29
US20240218378A1 (en) 2024-07-04
SG11202111693YA (en) 2021-11-29
JP2022531140A (ja) 2022-07-06
AU2020263580A1 (en) 2021-11-18
IL287418A (en) 2021-12-01
PH12021552674A1 (en) 2022-07-04
US12509695B2 (en) 2025-12-30

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